CN105378066A - 鉴定细胞群体、特别是间充质干细胞群体的多色流式细胞术方法 - Google Patents

鉴定细胞群体、特别是间充质干细胞群体的多色流式细胞术方法 Download PDF

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CN105378066A
CN105378066A CN201480039777.7A CN201480039777A CN105378066A CN 105378066 A CN105378066 A CN 105378066A CN 201480039777 A CN201480039777 A CN 201480039777A CN 105378066 A CN105378066 A CN 105378066A
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cell
cells
kit
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M·J·埃文斯
A·雷金纳德
S·苏丹
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Cell Therapy Ltd
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Cell Therapy Ltd
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705

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  • Urology & Nephrology (AREA)
  • Rheumatology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN201480039777.7A 2013-05-20 2014-05-20 鉴定细胞群体、特别是间充质干细胞群体的多色流式细胞术方法 Pending CN105378066A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1309057.6 2013-05-20
GBGB1309057.6A GB201309057D0 (en) 2013-05-20 2013-05-20 Method
PCT/GB2014/051538 WO2014188170A1 (en) 2013-05-20 2014-05-20 Multicolor flow cytometry method for identifying a population of cells, in particular mesenchymal stem cells

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CN105378066A true CN105378066A (zh) 2016-03-02

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CN201480039777.7A Pending CN105378066A (zh) 2013-05-20 2014-05-20 鉴定细胞群体、特别是间充质干细胞群体的多色流式细胞术方法

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US (1) US20160123980A1 (enExample)
EP (1) EP2999779A1 (enExample)
JP (1) JP2016519938A (enExample)
CN (1) CN105378066A (enExample)
AU (1) AU2014270111A1 (enExample)
CA (1) CA2912984A1 (enExample)
EA (1) EA201591926A1 (enExample)
GB (1) GB201309057D0 (enExample)
HK (1) HK1218310A1 (enExample)
SG (2) SG10201709214TA (enExample)
WO (1) WO2014188170A1 (enExample)

Cited By (9)

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CN106771205A (zh) * 2017-01-18 2017-05-31 浙江博真生物科技有限公司 十色抗体组合物及其在白血病淋巴瘤分型中的应用
CN107102127A (zh) * 2017-04-07 2017-08-29 东北师范大学 一种人体外周血中单核样髓源抑制细胞的检测方法
CN108865987A (zh) * 2018-07-20 2018-11-23 深圳市第二人民医院 一种人关节液间充质干细胞用的培养基及其制备方法
CN112147326A (zh) * 2020-09-04 2020-12-29 北京大学 一种肿瘤免疫细胞亚群分型精准检测试剂盒
CN112740042A (zh) * 2018-09-20 2021-04-30 文塔纳医疗系统公司 基于大小门控以分析流式细胞术数据
CN112881269A (zh) * 2021-02-23 2021-06-01 浙江正熙生物技术股份有限公司 一种流式荧光检测试剂的质量检测方法
CN114636824A (zh) * 2021-04-23 2022-06-17 西安康瑞道合生物科技有限公司 一种围产期脐带血中细胞因子和免疫细胞联合检测的试剂盒及其使用方法
CN114990058A (zh) * 2022-02-22 2022-09-02 厦门大学 一种脐带间充质干细胞功能亚群及其应用
CN115667923A (zh) * 2020-05-13 2023-01-31 贝克顿·迪金森公司 基于血液的络合物组对照物

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US10876953B2 (en) 2015-07-15 2020-12-29 Becton, Dickinson And Company System and method for label selection
US11639936B2 (en) * 2016-09-19 2023-05-02 Hematologics, Inc. System, method, and article for detecting abnormal cells using multi-dimensional analysis
GB201703058D0 (en) 2017-02-24 2017-04-12 Ucl Business Plc Biomarkers
KR102407387B1 (ko) * 2017-02-24 2022-06-10 가부시키가이샤 메토세라 섬유아세포를 포함하고, 심장 질환들의 치료를 위하여 사용될 수 있는 주사용 조성물, 및 치료 용도를 위하여 섬유아세포를 생산하는 방법
EP3399027A1 (en) * 2017-05-04 2018-11-07 Medizinische Hochschule Hannover Stem-cell derived myeloid cells, generation and use thereof
JPWO2018207918A1 (ja) * 2017-05-12 2020-03-26 国立大学法人 東京医科歯科大学 移植効率を向上させる間葉系幹細胞の純化方法
JP7595864B2 (ja) * 2020-07-14 2024-12-09 学校法人順天堂 細胞、組成物及び治療用組成物
CN116482378A (zh) * 2023-03-31 2023-07-25 上海吉量医药工程有限公司 一种检测间充质干细胞的荧光素标记抗体组合
WO2024227139A1 (en) * 2023-04-28 2024-10-31 University Of Florida Research Foundation, Incorporated Method for assessing the potency of cell populations in regenerative medicine using biomarkers
CN117517176B (zh) * 2024-01-04 2024-03-22 成都棱镜泰克生物科技有限公司 一种流式细胞数据自动处理方法及装置

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US7393656B2 (en) * 2001-07-10 2008-07-01 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for risk stratification
US8663935B2 (en) * 2008-12-12 2014-03-04 Beckman Coulter, Inc. Multicolor flow cytometry compositions containing unconjugated phycobiliproteins

Non-Patent Citations (2)

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JOLENE A. BRADFORD AND SCOTT T. CLARKE: "Panel Development for Multicolor Flow-Cytometry Testing of Proliferation and Immunophenotype in hMSCs", 《 MESENCHYMAL STEM CELL ASSAYS AND APPLICATIONS》 *
ULF PETRAUSCH,ET AL.: "Polychromatic Flow Cytometry: A Rapid Method for the Reduction and Analysis of Complex Multiparameter Data", 《CYTOMETRY PART A》 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106771205A (zh) * 2017-01-18 2017-05-31 浙江博真生物科技有限公司 十色抗体组合物及其在白血病淋巴瘤分型中的应用
CN107102127A (zh) * 2017-04-07 2017-08-29 东北师范大学 一种人体外周血中单核样髓源抑制细胞的检测方法
CN107102127B (zh) * 2017-04-07 2019-08-06 东北师范大学 一种人体外周血中单核样髓源抑制细胞的检测方法
CN108865987A (zh) * 2018-07-20 2018-11-23 深圳市第二人民医院 一种人关节液间充质干细胞用的培养基及其制备方法
CN112740042A (zh) * 2018-09-20 2021-04-30 文塔纳医疗系统公司 基于大小门控以分析流式细胞术数据
US12385922B2 (en) 2018-09-20 2025-08-12 Ventana Medical Systems, Inc. Size-based gating to analyze flow cytometry data
CN115667923A (zh) * 2020-05-13 2023-01-31 贝克顿·迪金森公司 基于血液的络合物组对照物
CN112147326A (zh) * 2020-09-04 2020-12-29 北京大学 一种肿瘤免疫细胞亚群分型精准检测试剂盒
CN112881269A (zh) * 2021-02-23 2021-06-01 浙江正熙生物技术股份有限公司 一种流式荧光检测试剂的质量检测方法
CN114636824A (zh) * 2021-04-23 2022-06-17 西安康瑞道合生物科技有限公司 一种围产期脐带血中细胞因子和免疫细胞联合检测的试剂盒及其使用方法
CN114990058A (zh) * 2022-02-22 2022-09-02 厦门大学 一种脐带间充质干细胞功能亚群及其应用
WO2023160566A1 (zh) * 2022-02-22 2023-08-31 厦门大学 一种脐带间充质干细胞功能亚群及其应用

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AU2014270111A1 (en) 2015-11-19
US20160123980A1 (en) 2016-05-05
GB201309057D0 (en) 2013-07-03
SG10201709214TA (en) 2017-12-28
WO2014188170A1 (en) 2014-11-27
EP2999779A1 (en) 2016-03-30
JP2016519938A (ja) 2016-07-11
EA201591926A1 (ru) 2016-06-30
HK1218310A1 (zh) 2017-02-10
CA2912984A1 (en) 2014-11-27
SG11201509536WA (en) 2015-12-30

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