CN105359971A - Paris polyphylla rhizome breeding method - Google Patents

Abstract

The invention relates to the technical field of plant breeding, in particular to a paris polyphylla rhizome breeding method. A wild paris polyphylla underground rhizome is dug out, soil on the surface of the rhizome is completely washed away, and after the rhizome is cut into blocks, the blocks are soaked in a D-fructose solution with the concentration of 500 ppm at the temperature of 8-12 DEG C for 24-28 hours; the rhizome blocks are placed in running water to be completely washed; the rhizome blocks are placed in a sterile culture medium and cultured at the temperature of 15-18 DEG C for 2-3 days, the rhizome blocks are completely washed under sterile water and placed in a rooting culture medium for rooting culture, the temperature of the rooting culture medium ranges from 20 DEG C to 22 DEG C, and illumination intensity ranges from 22 klx to 25 klx.

Description

A kind of paris polyphylla rhizome seedling-cultivating method

Technical field

The present invention relates to botanical seedling culturing technical field, be specifically related to a kind of paris polyphylla rhizome seedling-cultivating method.

Background technology

Paris polyphylla is the plant of Liliaceae Paris, flea stops another name, Chi stops, heavy platform root, whole stop, grass river car, heavy platform grass, Bai Gansui, RHIZOMA PARIDIS, worm beach wormwood, nine road hoops, mandarin duck worm, branch fresh idea, Calanthe davidii Franhc, conch seven, lamp stand seven, Omoto Nipponlily Rhizome, spiral shell top pseudo-ginseng, Sedum uizoon, have another name called schefflera arboricola, it is characterized in that emerging in the leaf verticillate by a circle flower, shape picture very much its leaf of flower, it can be divided into two parts, foreign steamer is colored and interior takes turns flower, foreign steamer flower with leaf the spitting image of, about there are 6, and interior flower of taking turns about has 8, the phyllotaxy of paris polyphylla belongs to verticillate leaves, sheet number has individual difference, have from 4 to 14, " seven leaves " be title just, the structure of flower is special, the leaf heart such as colyliform can be bloomed, calyx is green, petal is that filament is banded, Taiwan is used for controlling the symptom such as snake venom and sore swollen toxin in early days, paris polyphylla is that the Nature is presented greatly the mankind's one as natural crude drugs, but in recent years because people excessively excavate it, wild resource is caused to face the danger of extinction, so some plantation families start and carry out artificial planting to wild Paris polyphylla in recent years, in artificial planting process, seedling growing process is very crucial, it is long that current paris polyphylla nursery mainly also exists growing-seedling period, the shortcoming that survival rate of seedling is low.

Summary of the invention

The object of the present invention is to provide a kind of paris polyphylla rhizome seedling-cultivating method.

A kind of paris polyphylla rhizome seedling-cultivating method, comprises following plantation step:

1), November take robust growth, wild paris polyphylla underground rhizome without damage by disease and insect, its surperficial earth rinsed well, after being cut into bulk, concentration be 500ppm, temperature is soak 24-28 hour in the D-Fructose solution of 8-12 DEG C;

2), block rhizome is placed in flowing water rinses well;

3), block rhizome is placed in aseptic culture medium, 2-3 days is cultivated at 15-18 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts;

4), block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20-22 DEG C, illumination is 22-25klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, sphagna 30 parts, talcum powder 1.2 parts, 500 parts, water.

Rhizome seedling-cultivating method of the present invention can promote the fast healing and taking root of drop of water rhizome, improve the rooting rate of Yunnan Rhizoma Paridis rhizome segment, cultivate in root media and can grow healthy and strong root system in 15 days, can directly transplant to land for growing field crops after taking root, emerge after transplanting fast, emergence rate be high, emergence rate can reach 96.25%, the standardized quick fast-growing that can realize drop of water seedling is produced, and shortens crop cycle, increases economic efficiency.

Embodiment

Embodiment 1,1, take robust growth, wild paris polyphylla underground rhizome without damage by disease and insect November, its surperficial earth is rinsed well, after being cut into bulk, concentration be 500ppm, temperature is soak 24 hours in the D-Fructose solution of 8 DEG C; 2, block rhizome is placed in flowing water rinses well; 3, be placed in aseptic culture medium by block rhizome, cultivate 2 days at 15 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts; 4, block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20 DEG C, illumination is 22klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, sphagna 30 parts, talcum powder 1.2 parts, 500 parts, water.

Obtain block rhizome average cultivation in root media can take root for 15 days, rooting rate can reach 98.11%, and transplanting emergence rate to land for growing field crops can reach 95.12%.

Embodiment 2,1, take robust growth, wild paris polyphylla underground rhizome without damage by disease and insect November, its surperficial earth is rinsed well, after being cut into bulk, concentration be 500ppm, temperature is soak 26 hours in the D-Fructose solution of 10 DEG C; 2, block rhizome is placed in flowing water rinses well; 3, be placed in aseptic culture medium by block rhizome, cultivate 2.5 days at 16 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts; 4, block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 21 DEG C, illumination is 23klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, sphagna 30 parts, talcum powder 1.2 parts, 500 parts, water.

Obtain block rhizome average cultivation in root media can take root for 14.6 days, rooting rate can reach 98.65%, and transplanting emergence rate to land for growing field crops can reach 95.86%.

Embodiment 3,1, take robust growth, wild paris polyphylla underground rhizome without damage by disease and insect November, its surperficial earth is rinsed well, after being cut into bulk, concentration be 500ppm, temperature is soak 28 hours in the D-Fructose solution of 12 DEG C; 2, block rhizome is placed in flowing water rinses well; 3, be placed in aseptic culture medium by block rhizome, cultivate 3 days at 18 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts; 4, block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 22 DEG C, illumination is 25klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, sphagna 30 parts, talcum powder 1.2 parts, 500 parts, water.

Obtain block rhizome average cultivation in root media can take root for 14 days, rooting rate can reach 99.13%, and transplanting emergence rate to land for growing field crops can reach 96.25%.

Claims (1)

1. a paris polyphylla rhizome seedling-cultivating method, is characterized in that, it comprises following plantation step:

1), November take robust growth, wild paris polyphylla underground rhizome without damage by disease and insect, its surperficial earth rinsed well, after being cut into bulk, concentration be 500ppm, temperature is soak 24-28 hour in the D-Fructose solution of 8-12 DEG C;

2), block rhizome is placed in flowing water rinses well;

3), block rhizome is placed in aseptic culture medium, 2-3 days is cultivated at 15-18 DEG C, aseptic culture medium obtains after reconciling PH=5.2, sterilizing after being mixed by 15 parts, agar, D-(+)-glucopyranose 1 part, 6-BA0.02 part, distilled water 1000 parts;

4), block rhizome is rinsed well under sterile water, be placed in root media and carry out culture of rootage, culture of rootage temperature is 20-22 DEG C, illumination is 22-25klx, and root media is mixed by algae essence 1 part, shitosan 1 part, corn plumule powder 10 parts, potato starch 10 parts, vermiculite power 20 parts, sphagna 30 parts, talcum powder 1.2 parts, 500 parts, water.