CN105340883A - Aspergillus niger pellets - Google Patents
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- CN105340883A CN105340883A CN201510837671.8A CN201510837671A CN105340883A CN 105340883 A CN105340883 A CN 105340883A CN 201510837671 A CN201510837671 A CN 201510837671A CN 105340883 A CN105340883 A CN 105340883A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
- A01N25/28—Microcapsules or nanocapsules
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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Abstract
The invention relates to Aspergillus niger pellets which are used for preventing and treating konjac southern blight and soft rot. Aspergillus niger xj is separated by fungal resource institute of Guizhou university, is collected in CCTCC (China Center for Type Culture Collection) (address: Wuhan University, Wuhan, China) at present and has the collection number CCTCCNO of M206021. The Aspergillus niger pellets contain Aspergillus niger fermented concentrate crude extract powder, microcrystalline cellulose (MCC), lactose and hydroxypropyl methylcellulose and can be used for preventing and treating konjac southern blight and soft rot.
Description
Technical field
The present invention relates to biological technical field, particularly aspergillus niger (Aspergillusnigerxj) micropill and preparation method thereof.
Background technology
Aspergillus niger, Deuteromycotina, Hyphomycetes, hyphomycetales, Moniliaceae, a Common Species in aspergillus fungi.Be distributed widely in grain all over the world, plant product and soil.Be important fermentation industry bacterial classification, amylase, acid protease, cellulase, pectase, glucose oxidase, citric acid, gluconic acid and gallic acid etc. can be produced.The application is that the secondary metabolite utilizing aspergillus niger to produce in process of growth suppresses sclerotium rolfsii, Bacteria erwinia.Southern blight, also known as sclerotium root rot and sclerotium seedling blight, endangers the rhizome portion of nursery stock and treelet.This disease can endanger various plants, and the multiple seeds such as oil tea of mainly causing harm, tung oil tree, Chinese catalpa, citrus, apple, Chinese parasol tree, paulownia, walnut, masson pine, also can cause peanut, many crops morbidities such as shallot.Soft rot can make the tissue of plant or organ occur to rot.Germ is weak parasite, and the organ of the succulence plumpness of plant of mainly causing harm, as block root, stem tuber, fruit, stem foot etc.Morbidity is not limited to field, also have generation in transit, and it is heavier to cause harm with duration of storage.The crop species of causing harm is a lot of.The present invention is mainly for konjaku southern blight, soft rot.
Although fermentation of Aspergillus niger liquid has good antibacterial activity, but the unsuitable mass storage of the character because of its liquid and transport, as improper in preservation during long term storage more can by living contaminants, produce unknown harm to biocontrol effect and plant growth, therefore we have carried out further processing and optimization to fermentation of Aspergillus niger liquid product.
According to konjaku growth and the concrete condition of land for growing field crops biological and ecological methods to prevent plant disease, pests, and erosion, fermentation of Aspergillus niger liquid is processed into biological and ecological methods to prevent plant disease, pests, and erosion micropill sustained release agent.Micropill sustained release agent, based on slow controlled release theory, applies modern medicinal new accessories, technology of preparing and equipment and prepares the spherical or class spherical preparation of diameter within the scope of 0.5-1.5mm, plays and steadily, lastingly, safely and effectively acts on.Micropill can meet the Mlc of first dose requirements after directly using, also can produce the Mlc of maintenance dose, and Mlc curve is steady, and have repeatability, slow-release time reaches 72h.According to the feature of white thin,tough silk bacterium, soft rot growth and breeding, can Stable Release in the environment of high hot high humidity after the rain, effectively suppress the propagation of pathogen, the generation of konjaku southern blight, soft rot can be prevented when manpower and materials are limited to greatest extent.Adopt 30% fermentation of Aspergillus niger product micropill sustained release agent at effective dose 1.3g/m
3under to the average preventive effect of sclerotium rolfsii, Bacteria erwinia respectively 77.52%, 95.92%, there is stronger inhibitory action.The present inventor's object is to provide one to be easy to production, formulation, is beneficial to again the Antagonistic Fungi preparation of survival, uses it for konjaku southern blight, Disease-Control for Soft-Rotting.
Described Antagonistic Fungi is that aspergillus niger (Aspergillusnigerxj) is separated by fungus resource research institute of Guizhou University, be preserved in China typical culture collection center on March 7th, 2006, (address: Wuhan, China Wuhan University), preserving number: CCTCCNO:M206021
Summary of the invention
Technical problem to be solved by this invention solves stability and drug effect optimization, and realize aspergillus niger can Co ntrolled release.
The invention provides a kind of stable aspergillus niger micropill.
Present invention also offers the production method of described aspergillus niger micropill.
Present invention also offers the using method of described aspergillus niger micropill.
Present invention also offers described aspergillus niger micropill to can be used for preventing and treating southern blight, soft rot.
To achieve these goals, the invention provides a kind of Antagonistic Fungi, described Antagonistic Fungi is that aspergillus niger (Aspergillusnigerxj) is separated by fungus resource research institute of Guizhou University, be preserved in China typical culture collection center on March 7th, 2006, (address: Wuhan, China Wuhan University), preserving number: CCTCCNO:M206021.
For achieving the above object, the present invention adopts following technical proposals:
A kind of aspergillus niger micropill, it comprises following component: aspergillus niger, microcrystalline cellulose, lactose, hydroxypropyl methylcellulose.
Preferably, aspergillus niger is fermentation of Aspergillus niger concentrate crude extract pulvis.
Preferably, fermentation of Aspergillus niger concentrate crude extract pulvis 10 ~ 40 weight portion, microcrystalline cellulose 10 ~ 45 weight portion, lactose 15 ~ 50 weight portion and hydroxypropyl methylcellulose 1 ~ 10 weight portion.
Preferably, fermentation of Aspergillus niger concentrate crude extract pulvis 30 weight portion, microcrystalline cellulose 32 weight portion, lactose 35 weight portion and hydroxypropyl methylcellulose 3 weight portion.
Wherein, fermentation of Aspergillus niger concentrate crude extract pulvis is prepared by following method:
A. get sterilization fermentation liquid to filter, being concentrated into relative density at 25 DEG C is 1.12, spraying dry, and drying condition is: EAT 150 DEG C; Delivery temperature 95 DEG C; Flow velocity 20mL/s;
Or
B. get sterilization fermentation liquid to filter, being concentrated into relative density at 25 DEG C is 1.12, puts in 70 DEG C of baking ovens, forced air drying 36h, uses high speed disintegrator to pulverize after taking-up, crosses 100 mesh sieves and both obtains.
Described micropill can be used for control konjaku southern blight and soft rot.
When control konjaku southern blight: aspergillus niger micropill microbial inoculum and matrix are mixed by 1: 80 ~ 120, during plantation, microbial inoculum is filled with root, filled with root once every 30 days afterwards, after konjaku results, should spray 3-5 time in land for growing field crops large area.
When control konjak soft rot: aspergillus niger micropill microbial inoculum and matrix are mixed by 1: 400 ~ 600, during plantation, microbial inoculum is filled with root, filled with root once every 30 days afterwards.
Embodiment
The present invention is further described according to following embodiment, and those skilled in the art can understand, following embodiment only plays the effect of explanation to the present invention.Without departing from the premise in the spirit of the present invention, any improvement the present invention done and substituting all within the scope of protection of the invention.
The instrument used and reagent: machine for making small ball type granulating materials, mechanical industry research institute of East China University of Science; Standard inspection sieve, Sha Shai factory of Shangyu county of Zhejiang Province; CJY-300 friabilator, Shanghai Huanghai Sea medicine inspection instrument plant).Microcrystalline cellulose (MCC), Changshu pharmaceutic adjuvant factory; Lactose, Beijing extensive and profound in meaning star biotechnology responsibility Co., Ltd; Hydroxypropyl methylcellulose (HPMCK4M), Taian Shandong Rui Tai cellulose Co., Ltd; 95% ethanol, Shenyang reagent one factory; Described Antagonistic Fungi is that aspergillus niger (Aspergillusnigerxj) is provided by fungus resource research institute of Guizhou University, and its zymotic fluid conventionally middle method is prepared.
Embodiment 1: the preparation of sustained release pellet
Prepared by fermentation of Aspergillus niger concentrate crude extract pulvis:
A. get sterilization fermentation liquid to filter, being concentrated into relative density is 1.12 (25 DEG C), spraying dry, and drying condition is: EAT 150 DEG C; Delivery temperature 95 DEG C; Flow velocity 20mL/s.
Utilize spraying dry and EAT 150 DEG C; Delivery temperature 95 DEG C; Under flow velocity 20mL/s, concentrate drying processing is carried out to fermentation of Aspergillus niger liquid, adopt air after filtration and heating, enter drier headspace distributor, hot-air enters drying room in the shape of a spiral equably.Feed liquid is through the high speed centrifugal atomization device at tower body top, and (rotation) sprays into imperceptible vaporific liquid pearl, and contact with hot-air flow within the extremely short time drying to be finished product.Product has the good uniformity, mobility and dissolubility, and product purity is high, and quality is good.Production process simplifies, convenient operation and control.Liquid energy primary drying for water capacity 40-60% (special material can reach 90%) becomes powder and granule products, does not need to pulverize and screening after drying, reduces production process, improves product purity.To product cut size, bulk density, moisture content, adjust by changing operating condition within the specific limits, control and management is all very convenient.
Or
B. get sterilization fermentation liquid to filter, being concentrated into relative density is 1.12 (25 DEG C), puts in 70 DEG C of baking ovens, forced air drying 36h, uses high speed disintegrator to pulverize after taking-up, crosses 100 mesh sieves and both obtains.
Zymotic fluid high-temperature blast dry technology: utilize convection oven oven temperature 70 DEG C, forced air drying time 36h, carries out concentrate drying processing to fermentation of Aspergillus niger liquid.The multiple high-power blower of employing import of high-temperature blast drying oven, two duct type heat circulating system, is circulated stainless steel high temperature electric heater by wind, in Fast Heating operating room, make product dry rapidly, after taking-up, use high speed disintegrator to pulverize, cross 100 mesh sieves and both obtained.
Prepared by micropill:
Aspergillus niger micropill is made up of by weight percentage following component:
The 50mL ethanol water mixed with appropriate mass fraction is 30% that sieves obtains the moderate wet stock of humidity as binder granulation, is drying to obtain.
Embodiment 2: the preparation of sustained release pellet
The method identical according to embodiment 1 is prepared, and difference is the component adopting following weight parts:
The 50mL ethanol water mixed with appropriate mass fraction is 30% that sieves obtains the moderate wet stock of humidity as binder granulation, is drying to obtain.
Embodiment 3: the preparation of sustained release pellet
The method identical according to embodiment 1 is prepared, and difference is the component adopting following weight parts:
The 80mL ethanol water mixed with appropriate mass fraction is 30% that sieves obtains the moderate wet stock of humidity as binder granulation, is drying to obtain.
Embodiment 4: the quality evaluation of micropill dry jet mixing pile
Micropill quality evaluation parameter with particle diameter and domain size distribution, bulk density, configuration of surface and roundness, friability for index, except domain size distribution index, other each index all with the ball core of 20-24 order particle diameter for object is investigated.
Particle diameter and domain size distribution: adopt sieve analysis, the micropill under a series of sieve mesh of collection that final products are sieved, calculated yield.
Bulk density: get appropriate quantitatively ball core and make it slowly by a glass funnel, be poured onto in a graduated cylinder, measure the loose volume of micropill, calculate the bulk density of micropill.
Configuration of surface and roundness: observed the configuration of surface judging micropill by ocular estimate.To extrude micropill be bar-shaped marking is 1, and micropill is dumb-bell shape marking is 2, and micropill be that elliposoidal gives a mark is 3, and micropill marking spherical in shape is 4, gives a mark higher, and show that micropill configuration of surface is better, roundness is higher.Adopt the plane marginal stability (one-plane-critical-stability measuring micropill, be called for short OPCS) reflect roundness, 1g micropill is put on a flat board, dull and stereotyped side is lifted, measure the angle (Φ) that micropill starts to roll folded by top rake plane and level.Φ is less shows that roundness is better.
The mensuration at angle of repose: angle of repose is one of most popular method representing micropill mobility.The measurement at this experiment angle of repose is by quantitative ball core after specified altitude assignment falls hard plane from the funnel with diameter 1.25cm aperture, measures piling height (H) and the packing radius (r) of ball core, calculates tg α=H/r.α is angle of repose.By angle of repose indirect measure roundness.Angle of repose is less, illustrates that roundness is better.
Friability: friability weighs the index that micropill peels off trend.Assay method be get quantitative ball core to add 25 diameters be 7 ~ bead put together in friability instrument and rotate 10 minutes, collect and the weighed fine powder amount passing through 80 mesh sieves, calculate ball core percentage loss of weight.
Embodiment 5: the impact that technological factor is prepared micropill
With micropill prepared by embodiment 1, carry out the investigation experiment of following factor.
The impact of extruder extruded velocity: wet stock is placed on the funnel of shaft type single thread extrusion device, in 10,30, extrude under 40r/m, then putting round as a ball speed 1000r/m in spheronizator, after round as a ball 10min, collect micropill, micropill being put baking oven 40 DEG C of 12h to drying.Investigate extruded velocity on the surface state of wet stock, find time and the impact of micropill quality.When extruded velocity is 10r/m, certain material needs the overlong time extruded, and produces frictional heat many, makes the excessive result of the thermoplasticity of extrudate be bending, cannot round as a ball operation be carried out, when extruded velocity is at 30r/m and 40r/m, the quality of micropill not had a significant effect.Based on the consideration of economize energy and safety, choosing extruded velocity is 30r/m.
The impact of round as a ball speed: under the condition that other preparation technology is consistent, investigate extrudate 1000,1200, under the round as a ball speed of 1400r/m, quality evaluation is carried out to micropill.Round as a ball speed is remarkable to Size Distribution, and when round as a ball speed increases, the domain size distribution of micropill broadens and particle diameter becomes large.When round as a ball speeds control is at 1200r/m, target grain size 20 ~ 24 object micropill productive rate is the highest, reaches 82.45%.As round as a ball speed 1000r/m, micropill is dumb-bell shape, and outward appearance is poor, and the mobility of micropill is also slightly poor relative to other two condition, and target yield is 41.23%.Under this experiment condition, the friability of micropill is all less, meets the requirement of dressing to micropill mechanical strength.
The impact of round as a ball time: be 30r/m in extruder speed, under round as a ball speed 1200r/m condition, investigate respectively different round as a ball time 5,10,15min prepares the impact of result on micropill.The round as a ball time has a significant effect to particle diameter and roundness, is 30r/m at extruded velocity, and round as a ball speed is 1200r/m, and when the round as a ball time is 5min, extrudate only forms dumb-bell shape; When the time extends to 10min, the particle diameter of micropill is mainly distributed in 20-24 order and roundness improves, and target yield is 84.21%; But when the round as a ball time is 15min, there is more Large stone micropill.Along with the friability of the prolongation micropill of round as a ball time reduces.
The impact of wetting agent: be the impact that wetting agent investigates on micropill quality with water, 15% ethanol, 30% ethanol, 45% ethanol respectively.When making wetting agent with 10% ethanol, 20% ethanol, extrudate viscosity is too high, after round as a ball, what all have a large amount of micropill sticks on round as a ball pot wall, reason is that water viscosity is too large, and making storeroom create enough viscosity cannot come off from wall, causes round as a ball failure.It can thus be appreciated that the preparation of amount of moisture to micropill has a significant effect.When ethanol content reaches 30%, the particle diameter of micropill is mainly distributed in 20-24 order and roundness improves, and target yield is 85.84%.When the ethanolic solution of use 40%, round as a ball result is rod-shpaed particle, and friability is large; When the water yield increases, micropill particle diameter becomes large, and roundness improves, and friability reduces.Therefore select with the ethanolic solution of 30% as micropill prepared by wetting agent.
The control of fluid bed air blast flux: this experiment adopts hair-dryer to be 550w, and revolution 2700r/min, blow rate required 580L/min, blast is 1210Pa.Adopt and to add the screen cloth that baffle plate and fluid bed top are placed with certain pore size at hair-dryer place, regulate the size of air quantity in fluidisation cylinder, when make 30g micropill to be placed in fluid bed blow afloat 15-20cm time, baffle plate is positioned at apart from full closeding state 1.6cm, and the pore size of top mesh is 32 orders.
Embodiment 6: the impact that formula factors discharges micropill preparation and antibacterial material
The impact of balling-up supplementary product kind in formula: select MCC, MCC+HPMC (10: 1,11: 1,12: 1), crosslinked Na-CMC+MCC (1: 1,1: 2) be auxiliary material, add a certain amount of main ingredient in auxiliary material, moderate by the alcohol granulation humidity of 30%, the kind of investigation auxiliary material and consumption are on the impact of extruding with round as a ball rear micropill outward appearance and insoluble drug release.
The impact of MCC+HPMC (11: 1) and lactose usage ratio in formula: fixing fill a prescription in the amount of other composition, investigation MCC+HPMC and galactose ratio are respectively 1: 1,1: 1.5,1: 2 impact on micropill outward appearance and insoluble drug release.
The impact of water and lactose relative usage in formula: study and find, when water miscible lactose consumption increases, answer the consumption of corresponding minimizing water just can obtain the micropill of suitable size and roundness, otherwise micropill particle diameter obviously increase, target micropill yield reduces, but roundness improves.
Research finds, the MCC of Sq should be contained to ensure that wet stock has certain elasticity and plasticity in auxiliary material, the plasticity adding reduction material of lactose and elasticity, macromolecular material HPMC and crosslinked Na-CMC adds, and the viscosity of material and elasticity are improved, and the amplitude that crosslinked Na-CMC improves is larger.Be unfavorable for balling-up when HPMC and Na-CMC consumption increases, when increasing main ingredient amount, the viscoplasticity of available buffer mixed accessories, can avoid extrudate to bend, but only has Na-CMC+MCC can round as a ball balling-up, and prompting adds Na-CMC can make drugloading rate improve.From release result: all obtained than the MCC of the alone inertia micropill insoluble drug release that adds of the HPMC of water miscible lactose and water-swellable obtains soon, and crosslinked Na-CMC then makes insoluble drug release slightly slack-off.Known additionally by process in leaching, when making excipient with MCC, after stripping terminates, micropill still keeps original spherical, and when making excipient with MCC/HPMC (11: 1) and crosslinked Na-CMC/MCC (1: 2), micropill scatters and MCC/HPMC is swelling scatters faster.
Experimental result finds, along with the increase of lactose consumption, material elastoplasticity all reduces, micropill degradation, and when lactose yield increases to 1: 1.5, extrudate can not round as a ball globulate, and micropill is rod-shpaed particle.The amount increasing lactose makes faster drug release.When ratio is 1: 1, micropill roundness is best, and target yield reaches 84.87%.
Embodiment 7: release factors influencing
Impact under stripping oar different rotating speeds: by with micropill obtained under optimum formula and process conditions 50,75, paddle method is made release and is investigated under the rotating speed of 100r/m, measure the release of medicine, compare the impact of oar rotating speed on release.By with micropill obtained under best prescription and process conditions 50,75, paddle method is made release and is investigated under the rotating speed of 100r/m, measure the release of medicine, compare the impact of oar rotating speed on release.Experimental result is that oar rotating speed increases, and release is accelerated.During 100r/m, reach release peak value 91.21% at 26h.During 75r/m, reach peak value 86.32% at 35h.During 50r/m, reach peak value 84.56% at 41h.
The impact of different pH value medium: do release experiment by the dissolution medium being respectively 1.2,2.0,4.5,5.4,6.8 with micropill obtained under optimum formula and process conditions at H, investigate pH to the impact of release.On release impact significantly, when pH is greater than 4.5, insoluble drug release is obviously slack-off, and its reason is that pH value raises, the cause that drug solubility reduces for pH value.
The impact of different ionic strength medium: by with micropill obtained under optimum formula and process conditions respectively with 0.05,0.1,0.5, the KCI solution of 0.8mol/L makes dissolution medium, investigates ion strength to the impact of release.By with micropill obtained under best prescription and process conditions respectively with 0.05,0.1,0.5, the KCI solution of 0.8mol/L makes dissolution medium, investigates ion strength to the impact of release.Experiment shows, ion strength increases, and release is slack-off, and its drug release time is respectively 27.5h, 30.8h, 32.6h, 38h.
Vitro release assay method: take this product a certain amount of (about slightly carrying pulvis 120mg containing zymotic fluid) by drug release determination method paddle method device, respectively with distilled water, pH1.2 (0.1molL
-1hydrochloric acid) and pH6.8, pH7.4 phosphate buffer (PBS) 900mL be solvent, rotating speed is 100rmin
-1, operate in accordance with the law.Get solution 5mL respectively at different time to filter, and instant in process container supplementing solvent 5mL, get subsequent filtrate, according to spectrophotometric method, measure trap at 355nm wavelength place, calculate the Accumulation dissolution of different time respectively.
The impact of coating weight gain: respectively with 5%, 10%, 15%HPMC adds in the coating solution of NE: L (10: 1), 8%, 10%, 15% dressing is carried out to plain ball, investigates the impact on insoluble drug release.Along with the increase of dressing amount, insoluble drug release slows down, and all occur time delay as coating weight gain > 10% under each condition, during coating weight gain 15%, time lag reaches 2 hours.10%HPMC drug release time when coating weight gain is 8% is little with 15%HPMC drug release time difference when coating weight gain is 10%, but the latter occurred time lag at first hour.This prompting: coating weight gain is the principal element causing time lag, and when coating weight gain is large, adding of high consumption pore-foaming agent can not improve time delay, so the means solving Time Delay are consumptions of suitably less pore-foaming agent while of reducing dressing amount.Consider economic factor, the weightening finish of this micropill formulation selection coating solution is 8%.
In micropill preparation, the consumption of round as a ball speed, round as a ball time and wetting agent is the key factor of technique; In prescription, can the usage ratio of MCC/HPMC and lactose be the principal element that obtain the measured micropill of matter, and lactose is as the conditioning agent of insoluble drug release.Determine preparation technology and prescription composition.Finally show that technique and the formula of making sustained release pellet are: Fermented Condensed liquid crude extract pulvis 30 weight portion, microcrystalline cellulose (MCC) 30 weight portion, lactose 30 weight portion, hydroxypropyl methylcellulose (HPMCK4M) 10 weight portion, 30% ethanol.Zymotic fluid is slightly carried pulvis and auxiliary material and to be sieved mixing by formula ratio, add mass fraction be 30% ethanol water be 30r/m as binder, extruder speed, round as a ball speed 1200r/m, round as a ball time 10min, coating solution increase weight under 8% condition, can prepare proterties better, average grain diameter is the sustained release pellet of about 1.5mm.Weight-loss method measurement result shows, its sustained release performance is good.Adopt 30% fermentation of Aspergillus niger product micropill sustained release agent at effective dose 1.3g/m
3under to the average preventive effect of konjaku sclerotium rolfsii, Bacteria erwinia respectively 77.52%, 95.92%, there is stronger inhibitory action.
Consider according to hydroscopicity and molding effect, sustained release pellet has selected MCC, lactose, hydroxypropyl methylcellulose (HPMCK4M) as auxiliary material.
Embodiment 8: control konjaku southern blight
The aspergillus niger micropill microbial inoculum of embodiment 2 and matrix are mixed by 1: 100.During plantation, microbial inoculum is filled with root, fill with root once every 30 days afterwards.After konjaku results.Should spray 3-5 time in land for growing field crops large area, to stop the germination by repetition in sclerotium coming year of sclerotium rolfsii formation.
Fermentation of Aspergillus niger liquid and matrix (in prior art conventional matrix) mix by 1: 100, and other service condition is identical with micropill.
Conventional matrix formulations is: peat, perlite, gardening vermiculite (by volume) 1: 1: 1.Or 7 parts of peat+3 parts of vermiculites.
Aspergillus niger micropill microbial inoculum is carried out 300 mu apply.Through field actual measurement, in disease resistance, the check plot incidence of disease 30.59%, the treatment region incidence of disease 8.54%.And average preventive effect reaches 77.52%, have and obviously prevent and treat konjaku southern blight effect, the incidence of disease sees the following form.
Before sowing, investigation kind of a taro is rotted the order of severity respectively.Southern blight Seriousness gradation standard
0 grade: disease-free symptom
1 grade: onset area accounts for (the 0 < X < 5%) of kind of taro area
2 grades: onset area accounts for (the 5% < X < 15%) of kind of taro area
3 grades: onset area accounts for (the 15% < X < 30%) of kind of taro area
4 grades: onset area accounts for (the 30% < X < 50%) of kind of taro area
5 grades: onset area accounts for (the X > 50%) of kind of taro area
Embodiment 9: control konjak soft rot
The aspergillus niger micropill microbial inoculum of embodiment 1 and matrix are mixed by 1: 500.During plantation, microbial inoculum is filled with root, fill with root once every 30 days afterwards.
Fermentation of Aspergillus niger liquid and matrix mix by 1: 500, and other service condition is identical with micropill.
Conventional matrix formulations is: peat, perlite, gardening vermiculite (by volume) 1: 1: 1.Or 7 parts of peat+3 parts of vermiculites.
Aspergillus niger micropill microbial inoculum is carried out 300 mu apply.Through field actual measurement, in disease resistance, check plot diseased plant rate 10.49%, the treatment region incidence of disease 0.33%.And average preventive effect reaches 95.93%, have and especially significantly prevent and treat konjak soft rot effect, the incidence of disease sees the following form.
Before sowing, investigation kind of a taro is rotted the order of severity respectively.Soft rot Seriousness gradation standard
0 grade: disease-free symptom
1 grade: bulb web rot area accounts for (the 0 < X < 1/3) of bulb area
2 grades: bulb web rot area accounts for (the 1/3 < X < 1/2) of bulb area
3 grades: bulb web rot area accounts for (the 1/2 < X) of bulb area
。
Claims (8)
1. an aspergillus niger micropill, is characterized in that comprising following component: aspergillus niger, microcrystalline cellulose, lactose, hydroxypropyl methylcellulose.
2. micropill according to claim 1, is characterized in that described aspergillus niger is fermentation of Aspergillus niger concentrate crude extract pulvis.
3. micropill according to claim 2, is characterized in that containing: fermentation of Aspergillus niger concentrate crude extract pulvis 10 ~ 40 weight portion, microcrystalline cellulose 10 ~ 45 weight portion, lactose 15 ~ 50 weight portion and hydroxypropyl methylcellulose 1 ~ 10 weight portion.
4. micropill according to claim 3, is characterized in that containing: fermentation of Aspergillus niger concentrate crude extract pulvis 30 weight portion, microcrystalline cellulose 32 weight portion, lactose 35 weight portion and hydroxypropyl methylcellulose 3 weight portion.
5. micropill according to claim 2, is characterized in that described fermentation of Aspergillus niger concentrate crude extract pulvis is prepared by following method:
A. get sterilization fermentation liquid to filter, being concentrated into relative density at 25 DEG C is 1.12, spraying dry, and drying condition is: EAT 150 DEG C; Delivery temperature 95 DEG C; Flow velocity 20mL/s;
Or
B. get sterilization fermentation liquid to filter, being concentrated into relative density at 25 DEG C is 1.12, puts in 70 DEG C of baking ovens, forced air drying 36h, uses high speed disintegrator to pulverize after taking-up, crosses 100 mesh sieves and both obtains.
6. the arbitrary described micropill of claim 1-5 is preventing and treating the application of konjaku southern blight and soft rot.
7. the using method of the arbitrary described micropill of claim 1-5, it is characterized in that when control konjaku southern blight: aspergillus niger micropill microbial inoculum and matrix are mixed by 1: 80 ~ 120, during plantation, microbial inoculum is filled with root, root is filled with once afterwards every 30 days, after konjaku results, should spray 3-5 time in land for growing field crops large area.
8. the using method of the arbitrary described micropill of claim 1-5, is characterized in that when control konjak soft rot: aspergillus niger micropill microbial inoculum and matrix are mixed by 1: 400 ~ 600, during plantation, microbial inoculum is filled with root, fill with root once afterwards every 30 days.
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| CN110604033A (en) * | 2019-11-05 | 2019-12-24 | 隆尧怡东农牧有限公司 | Preparation method and application of matrix for preventing and treating soft rot of vegetables |
| CN113631251A (en) * | 2019-03-15 | 2021-11-09 | 诺和诺德股份有限公司 | Spray drying process of GLP-1 peptide |
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| CN101967455A (en) * | 2009-12-31 | 2011-02-09 | 湖北省农业科学院植保土肥研究所 | Bacillus amyloliquefaciens EA19 for controlling wheat root diseases and preparation thereof |
| CN102630401A (en) * | 2012-04-19 | 2012-08-15 | 重庆市彭水县天娇农业开发有限公司 | Method for preventing disease of konjac |
| CN103773718A (en) * | 2013-12-30 | 2014-05-07 | 邵素英 | Novel preparation method of micro-ecological fertilizer |
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| CN101967455A (en) * | 2009-12-31 | 2011-02-09 | 湖北省农业科学院植保土肥研究所 | Bacillus amyloliquefaciens EA19 for controlling wheat root diseases and preparation thereof |
| CN102630401A (en) * | 2012-04-19 | 2012-08-15 | 重庆市彭水县天娇农业开发有限公司 | Method for preventing disease of konjac |
| CN103773718A (en) * | 2013-12-30 | 2014-05-07 | 邵素英 | Novel preparation method of micro-ecological fertilizer |
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| CN113631251A (en) * | 2019-03-15 | 2021-11-09 | 诺和诺德股份有限公司 | Spray drying process of GLP-1 peptide |
| CN110604033A (en) * | 2019-11-05 | 2019-12-24 | 隆尧怡东农牧有限公司 | Preparation method and application of matrix for preventing and treating soft rot of vegetables |
| CN110604033B (en) * | 2019-11-05 | 2021-08-24 | 隆尧怡东农牧有限公司 | Preparation method and application of matrix for preventing and treating soft rot of vegetables |
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