CN105340753A - High-starch-yield potato tissue culture method - Google Patents
High-starch-yield potato tissue culture method Download PDFInfo
- Publication number
- CN105340753A CN105340753A CN201510878795.0A CN201510878795A CN105340753A CN 105340753 A CN105340753 A CN 105340753A CN 201510878795 A CN201510878795 A CN 201510878795A CN 105340753 A CN105340753 A CN 105340753A
- Authority
- CN
- China
- Prior art keywords
- culture
- virus
- tissue culture
- starch
- high yield
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention relates to the field of agricultural breeding, in particular to a high-starch-yield potato tissue culture method which comprises the following operating steps: (1) virus-free culture for 2-4 times; (2) rooting culture; (3) seedling hardening culture; (4) shifting and planting, wherein a special virus-free culture medium is selected for the virus-free culture, and prepared by adopting an MS culture medium as a basis, and adding 0.1 mg/L of IAA, 0.1 mg/L of 6-furfurylaminopurine and 0.2 mg/L of C25H25O6N2Zn. Through the adoption of the high-starch-yield potato tissue culture method, the starch content of potatoes can be improved by 50%, and the planting cost of farmers can be reduced when the production benefits of the potato starch industry are greatly increased.
Description
Technical field
The present invention relates to agricultural breeding field, be specifically related to a kind of method for tissue culture of high yield starch potato.
Background technology
Potato is as one of large staple food grain of China five, and it is of high nutritive value, adaptive faculty is strong, output is large, is the third-largest important cereal crops in the whole world, is only second to wheat and maize.Potato is except containing except protein, and also containing abundant carbohydrate, every hectogram up to 16.8 grams, and contains the mineral matters such as abundant vitamin C, vitamin A, vitamin B1 B2 and calcium, phosphorus, iron.It is also containing vitamin B6 simultaneously, and vitamin B6 prevents arteriosclerotic effect.So normal food potato can reduce the incidence of disease of cerebral hemorrhage.The nutritive value of potato is very high, and high-quality starch content is about 16.5%, and the starch in potato absorbs slowly in vivo, can not cause the too fast rising of blood sugar.Also containing a large amount of lignin etc., be described as " second bread " of the mankind.Containing a large amount of good fiber quality element in potato, can be used for a large amount of nutrition of enteric microorganism in enteron aisle, promote that enteric microorganism grows.Can also intestines peristalsis be promoted simultaneously, keep enteron aisle moisture, have the effect such as Constipation and anti-curing cancers.
Wherein, potato starch is widely used in the industries such as weaving, oil exploitation, feed and food, the developing of especially international and domestic food products market, the demand of high-precision potato starch is surged, in addition potato starch has the irreplaceable natural quality of other starch, become the first-selected product of domestic and international starch deep processing industry, market is wide, market prospects are good.And data show that the edible way more than 90% of potato is to eat raw in China, the dormancy time limit of normal temperature Potato is approximately 2-5 month, bud will be grown from stem tuber sprout once cross resting stage potato, because potato produces a kind of extremely toxic substance around the eye germinateed, be called solanine, people eats will be poisoning.Therefore a large amount of potatos all can be had every year to abandon because of the last corruption of unsalable germination in China, country is according to the market demand, stand in strategic height and potato is promoted to one of five large staple food grains, strengthen the input to potato especially its starch deep processing and guiding, and the high-quality starch content of domestic potato is mostly between 13-18%, general lower, processing benefit is not good enough, therefore needs the potato culture method finding high yield starch.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for tissue culture of high yield starch potato.
Technical problem to be solved by this invention is achieved by the following technical programs:
A method for tissue culture for high yield starch potato, described method for tissue culture comprises following operating procedure: the 1. virus-free culture of 2-4 time; 2. culture of rootage; 3. practice seedling to cultivate; 4. transfer plantation; Described virus-free culture selects special virus-free culture base, and described virus-free culture base, based on MS medium, adds IAA0.1mg/L, KT 0.1mg/L and C
25h
25o
6n
2zn0.2mg/L is prepared from.
Further, described C
25h
25o
6n
2zn is anorthic system,
p-1space group, cell parameter is
a=13.108 (3),
b=16.0526 (4),
c=12.929 (7),
α=86.839 (3) o,
β=103.483 (3) o,
γ=88.237 (5) o, V=2716.49 (4)
3.
C
25h
25o
6n
2the preparation method of Zn is:
By 1mmolN
1, N
8-two (pyridin-4-yl) suberamide (its chemical structural formula is as follows), 2mmol diphenyl methane-4,4'-dicarboxylic acids and 2mmol zinc nitrate are dissolved in (wherein the volume ratio of ethanol and carrene is 2:5) in the mixed solvent of ethanol and carrene and obtain mixed liquor, are then left standstill at 35 DEG C by above-mentioned mixed liquor and obtain above-mentioned C in more than 5 days
25h
25o
6n
2zn.
Further, the terminal bud 0.2mm size that described virus-free culture strips potato plant terminal bud or stem tuber sprouting by anatomical lens is explant, and sterilization is placed on virus-free culture base cultivates, and within every 16 days, subculture is sheared once, repeatedly obtains detoxification test tube plantlet 2-4 time.
Further, described culture of rootage uses root media, and described root media uses shallow-layer liquid MS medium; Wherein replace sucrose with white sugar, running water replaces distilled water, and cultivation temperature 22 DEG C-28 DEG C, illumination 12-14h, intensity of illumination 1300lux-1600lux, culture of rootage 6-8d or root are about 1-2cm, can carry out practicing seedling.
Further, it is under blake bottle is placed on natural lighting and ventilation condition that described experienced seedling is cultivated, bottle cap is opened experienced seedling 2-3 days, in white silk seedling process, intensity of illumination can not higher than 3500lux, sunshade net is used to regulate when sunray is stronger, and keep air humidity in process more than 75%, and sprayed water once with thin eye watering can every 2-3 hour by day.
Further, described transfer plantation, for be taken out from blake bottle by test-tube plantlet, washes away the medium of root with clear water, is more carefully inserted in paper web with tweezers and cultivates; Two weeks after transfer needs cover film to be incubated, and makes night minimum temperature be not less than 16 DEG C, has new leaf growth to be unearthed and adds sunshade net sunshade two weeks.
The present invention has following beneficial effect:
The method for tissue culture of high yield starch potato of the present invention, can make the content of starch of potato promote and reach 50%, with the planting cost also reducing peasant household while significantly increasing potato starch industry productivity effect.
Accompanying drawing explanation
1. Fig. 1 is C
25h
25o
6n
2zn chemical structural formula.
Embodiment
The present invention will be described in detail for specific embodiment below.
Embodiment:
A method for tissue culture for high yield starch potato, described method for tissue culture comprises following operating procedure: the 1. virus-free culture of 2-4 time; 2. culture of rootage; 3. practice seedling to cultivate; 4. transfer plantation; Described virus-free culture selects special virus-free culture base, and described virus-free culture base, based on MS medium, adds IAA0.1mg/L, KT 0.1mg/L and C
25h
25o
6n
2zn0.2mg/L is prepared from.Described C
25h
25o
6n
2zn all synthesizes with following methods:
By 1mmolN
1, N
8-two (pyridin-4-yl) suberamide, 2mmol diphenyl methane-4,4'-dicarboxylic acids and 2mmol zinc nitrate are dissolved in the mixed solvent of 2mL ethanol and 5mL carrene and obtain mixed liquor, are then left standstill at 35 DEG C by above-mentioned mixed liquor and obtain above-mentioned C in more than 5 days
25h
25o
6n
2zn crystal (productive rate is 85%, based on Zn).
C
25h
25o
6n
2the X ray diffracting data of Zn visits on diffractometer in BrukerSmartApexCCD face, uses MoK
αradiation (λ=0.71073), collects with ω scan mode and carries out Lp factor correction, and absorption correction uses SADABS program.Use direct method solution structure, then obtain whole non-hydrogen atom coordinate by difference Fourier method, and obtain hydrogen atom position (C H1.083) with theoretical hydrogenation method, by method of least squares, structure is revised.Evaluation work completes with SHELXTL program package on PC.
Resolve known after tested, chemical molecular formula is C
25h
25o
6n
2zn is anorthic system,
p-1space group, cell parameter is
a=13.108 (3),
b=16.0526 (4),
c=12.929 (7),
α=86.839 (3) o,
β=103.483 (3) o,
γ=88.237 (5) o, V=2716.49 (4)
3, Z=4, its chemical structural formula as shown in Figure 1.
The terminal bud 0.2mm size that described virus-free culture strips potato plant terminal bud or stem tuber sprouting by anatomical lens is explant, and sterilization is placed on virus-free culture base cultivates, and within every 16 days, subculture is sheared once, repeatedly obtains detoxification test tube plantlets 3 times.
Described culture of rootage uses root media, and described root media uses shallow-layer liquid MS medium; Wherein replace sucrose with white sugar, running water replaces distilled water, and cultivation temperature 22 DEG C-28 DEG C, illumination 13h, intensity of illumination 1500lux, culture of rootage 7d or root are about 1-2cm, can carry out practicing seedling.
It is under blake bottle is placed on natural lighting and ventilation condition that described experienced seedling is cultivated, bottle cap is opened experienced seedling 2-3 days, in white silk seedling process, intensity of illumination can not higher than 3500lux, sunshade net is used to regulate when sunray is stronger, and keep air humidity in process more than 75%, and sprayed water once with thin eye watering can every 2-3 hour by day.
Described transfer plantation, for be taken out from blake bottle by test-tube plantlet, washes away the medium of root with clear water, is more carefully inserted in paper web with tweezers and cultivates; Two weeks after transfer needs cover film to be incubated, and makes night minimum temperature be not less than 16 DEG C, has new leaf growth to be unearthed and adds sunshade net sunshade two weeks.
Comparative example:
A method for tissue culture for high yield starch potato, described method for tissue culture comprises following operating procedure: the 1. virus-free culture of 2-4 time; 2. culture of rootage; 3. practice seedling to cultivate; 4. transfer plantation; Described virus-free culture selects special virus-free culture base, and described virus-free culture base, based on MS medium, adds IAA0.1mg/L, KT 0.1mg/L.
Comparative example operation is identical with embodiment, and difference is in virus-free culture base not containing C
25h
25o
6n
2zn.
Experimental example:
The test-tube plantlet (experimental group) cultivate the present embodiment and the test-tube plantlet (control group) of comparative example are planted in one piece of square sample plot simultaneously, experimental field will be divided into four parts of area equation by sphere of movements for the elephants sample, get two pieces, diagonal angle and plant test-tube plantlet of the present invention and conventional detoxification test tube plantlet respectively, manage according to same way to manage after plantation, gather in the crops the contrast of laggard line sampling: experimental group and control group respectively randomly draw potato three groups, 50 kilograms often group carry out starch yield contrast, obtain data be prepared as follows table:
Through overtesting, the potato starch output that the inventive method is produced can account for about 23.2% of potato deadweight; Compared to the potato of conventional method plantation, the potato of equal in quality, starch output can increase about 50%.
The above embodiment only have expressed embodiments of the present invention; it describes comparatively concrete and detailed; but therefore can not be interpreted as the restriction to the scope of the claims of the present invention; in every case the technical scheme adopting the form of equivalent replacement or equivalent transformation to obtain, all should drop within protection scope of the present invention.
Claims (6)
1. a method for tissue culture for high yield starch potato, is characterized in that: described method for tissue culture comprises following operating procedure: the 1. virus-free culture of 2-4 time; 2. culture of rootage; 3. practice seedling to cultivate; 4. transfer plantation; Described virus-free culture selects special virus-free culture base, and described virus-free culture base, based on MS medium, adds IAA0.1mg/L, KT 0.1mg/L and C
25h
25o
6n
2zn0.2mg/L is prepared from.
2. the method for tissue culture of a kind of high yield starch potato according to claim 1, is characterized in that being: C
25h
25o
6n
2zn is anorthic system,
p-1space group, cell parameter is
a=13.108 (3),
b=16.0526 (4),
c=12.929 (7),
α=86.839 (3) o,
β=103.483 (3) o,
γ=88.237 (5) o, V=2716.49 (4)
3.
3. the method for tissue culture of a kind of high yield starch potato according to claim 2, it is characterized in that being: the terminal bud 0.2mm size that described virus-free culture strips potato plant terminal bud or stem tuber sprouting by anatomical lens is explant, sterilization is placed on virus-free culture base cultivates, within every 16 days, subculture is sheared once, repeatedly obtains detoxification test tube plantlet 2-4 time.
4. the method for tissue culture of a kind of high yield starch potato according to claim 3, is characterized in that: described culture of rootage uses root media, and described root media uses shallow-layer liquid MS medium; Wherein replace sucrose with white sugar, running water replaces distilled water, and cultivation temperature 22 DEG C-28 DEG C, illumination 12-14h, intensity of illumination 1300lux-1600lux, culture of rootage 6-8d or root are about 1-2cm, can carry out practicing seedling.
5. the method for tissue culture of a kind of high yield starch potato according to claim 4, it is characterized in that: it is under blake bottle is placed on natural lighting and ventilation condition that described experienced seedling is cultivated, bottle cap is opened experienced seedling 2-3 days, in white silk seedling process, intensity of illumination can not higher than 3500lux, sunshade net is used to regulate when sunray is stronger, and keep air humidity in process more than 75%, and sprayed water once with thin eye watering can every 2-3 hour by day.
6. the method for tissue culture of a kind of high yield starch potato according to claim 5, it is characterized in that: described transfer plantation is for take out test-tube plantlet from blake bottle, wash away the medium of root with clear water, be more carefully inserted in paper web with tweezers and cultivate; Two weeks after transfer needs cover film to be incubated, and makes night minimum temperature be not less than 16 DEG C, has new leaf growth to be unearthed and adds sunshade net sunshade two weeks.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510878795.0A CN105340753A (en) | 2015-12-04 | 2015-12-04 | High-starch-yield potato tissue culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510878795.0A CN105340753A (en) | 2015-12-04 | 2015-12-04 | High-starch-yield potato tissue culture method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105340753A true CN105340753A (en) | 2016-02-24 |
Family
ID=55317043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510878795.0A Pending CN105340753A (en) | 2015-12-04 | 2015-12-04 | High-starch-yield potato tissue culture method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105340753A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105687129A (en) * | 2016-03-11 | 2016-06-22 | 广东伊茗药业有限公司 | BDZL (bendazac lysine) eye drops |
| CN111374051A (en) * | 2018-12-31 | 2020-07-07 | 甘肃康勤薯业有限公司 | Cultivation method of potatoes in net shed |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011083235A (en) * | 2009-10-16 | 2011-04-28 | Kinjirushi Kk | Method for forming multiple shoot from culture seedling and method for producing virus-free seed yam |
| CN102577960A (en) * | 2012-02-27 | 2012-07-18 | 江阴沃土农业生物科技开发有限公司 | Tissue culture method of potatoes |
| CN103329805A (en) * | 2013-06-28 | 2013-10-02 | 江苏开昌农业综合开发科技有限公司 | Simplified potato detoxification method |
-
2015
- 2015-12-04 CN CN201510878795.0A patent/CN105340753A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011083235A (en) * | 2009-10-16 | 2011-04-28 | Kinjirushi Kk | Method for forming multiple shoot from culture seedling and method for producing virus-free seed yam |
| CN102577960A (en) * | 2012-02-27 | 2012-07-18 | 江阴沃土农业生物科技开发有限公司 | Tissue culture method of potatoes |
| CN103329805A (en) * | 2013-06-28 | 2013-10-02 | 江苏开昌农业综合开发科技有限公司 | Simplified potato detoxification method |
Non-Patent Citations (3)
| Title |
|---|
| MIASSAR M. AL-TALEB ET.AL.,: "Production of Virus Free Potato Plants Using Meristem Culture from Cultivars Grown under Jordanian Environment", 《AMERICAN-EURASIAN J. AGRIC. & ENVIRON. SCI.,》 * |
| 孙秀梅: "马铃薯茎尖剥离脱毒效果的影响因素分析", 《中国马铃薯》 * |
| 王晓杰: "马铃薯微型薯切块离体再生的研究", 《青岛农业大学学报（自然科学版）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105687129A (en) * | 2016-03-11 | 2016-06-22 | 广东伊茗药业有限公司 | BDZL (bendazac lysine) eye drops |
| CN111374051A (en) * | 2018-12-31 | 2020-07-07 | 甘肃康勤薯业有限公司 | Cultivation method of potatoes in net shed |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110521566A (en) | A kind of luminous environment regulation method of regulation plant metabolism substance | |
| CN103060156A (en) | Production process of Hibiscus manihot wine | |
| CN111543300A (en) | Light environment regulation and control method for promoting lettuce vegetable core wrapping | |
| CN108901605A (en) | A kind of cultural method of seafood mushroom | |
| CN105248279A (en) | Tissue culture method for miniature potatoes | |
| CN105340753A (en) | High-starch-yield potato tissue culture method | |
| KR101908518B1 (en) | A Method of Cultivation of Stevia Onion Using Stevia Liquid Compost And Stevia Onion Liquid Thereof | |
| CN103416310B (en) | Culture medium used for roxburgh anoectochilus terminal bud organic tissue culture | |
| CN110583280B (en) | Luminous environment regulation and control method for reducing lettuce cooking heart rate in plant factory | |
| CN103194524B (en) | Method for rapidly identifying peanut germplasm with bacterial wilt resistance | |
| CN105359950A (en) | Germination-accelerating culture technology of peanuts | |
| CN113057078A (en) | Method for improving yield of flowering cabbage by using soil conditioner | |
| Storozhyk et al. | Allelopathic potential of sugar sorghum (Sorghum bicolor (L.) Moench) seeds. | |
| CN113678830B (en) | Composition for improving watermelon fruit quality and application thereof | |
| CN106417019B (en) | A kind of propagation method and culture medium of Vitis davidii Foex seedling | |
| Horibe et al. | Effects of Light Wavelength on Daughter Cladode Growth and Quality in Edible Cactus Cultured in a Plant Factory with Artificial Light | |
| CN112655562B (en) | Method for promoting germination of test-tube taro of red-bud taro | |
| CN108770666A (en) | A kind of method of whole process full-spectrum LED light source vegetable aquaculture | |
| CN111732469A (en) | Organic water culture nutrient solution and application thereof | |
| CN107996304B (en) | Temperature regulation and control method for increasing celery petiole apigenin content | |
| CN106995327A (en) | The formula and preparation method of a kind of edible mushroom main pipe culture medium | |
| Pyshnaya et al. | Resistance of sweet pepper genotypes to abiotic stresses in grpwing conditions of low-capacity hydroponics | |
| CN109738596A (en) | A method of diagnosis Ca in Tomato element nutrition condition | |
| CN105532449A (en) | Virus free culture medium of potato | |
| Cheng et al. | Physiological, anatomical and quality indexes of root tuber formation and development in chayote (Sechium edule) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160224 |