CN105326873A - Method for extracting clinacanthus nutans active substances through ultrasonic assistance - Google Patents
Method for extracting clinacanthus nutans active substances through ultrasonic assistance Download PDFInfo
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- CN105326873A CN105326873A CN201510722739.8A CN201510722739A CN105326873A CN 105326873 A CN105326873 A CN 105326873A CN 201510722739 A CN201510722739 A CN 201510722739A CN 105326873 A CN105326873 A CN 105326873A
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- 239000013543 active substance Substances 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 23
- 241000123852 Clinacanthus nutans Species 0.000 title abstract 7
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 238000000227 grinding Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 244000025254 Cannabis sativa Species 0.000 claims description 38
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 18
- 235000012054 meals Nutrition 0.000 claims description 17
- 238000000605 extraction Methods 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 9
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims description 9
- 229930003268 Vitamin C Natural products 0.000 claims description 9
- 229930003944 flavone Natural products 0.000 claims description 9
- 235000011949 flavones Nutrition 0.000 claims description 9
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 9
- 235000013824 polyphenols Nutrition 0.000 claims description 9
- 150000003648 triterpenes Chemical class 0.000 claims description 9
- 235000019154 vitamin C Nutrition 0.000 claims description 9
- 239000011718 vitamin C Substances 0.000 claims description 9
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 9
- 150000002212 flavone derivatives Chemical class 0.000 claims description 8
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000002137 ultrasound extraction Methods 0.000 claims 3
- 238000005119 centrifugation Methods 0.000 claims 1
- 239000012153 distilled water Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 8
- 230000001105 regulatory effect Effects 0.000 abstract description 6
- 239000000843 powder Substances 0.000 abstract 4
- 239000000243 solution Substances 0.000 abstract 2
- 239000010414 supernatant solution Substances 0.000 abstract 1
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
- 238000002604 ultrasonography Methods 0.000 description 7
- 239000000284 extract Substances 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 208000004880 Polyuria Diseases 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 230000035619 diuresis Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 206010030113 Oedema Diseases 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- -1 flavone glycoside Chemical class 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002435 venom Substances 0.000 description 1
- 210000001048 venom Anatomy 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting clinacanthus nutans active substances through ultrasonic assistance. The method mainly comprises the steps that 1, raw materials are cleaned up, drained, dried, ground through a superfine grinding mill and screened with a 200-mesh sieve, and then clinacanthus nutans powder is obtained; 2, after being subpackaged, the clinacanthus nutans powder is placed into a refrigerator and preserved at 5 DEG C for standby application; 3, the ultrasonic power, ultrasonic temperature and ultrasonic time of an ultrasonic cleaner are regulated; 4, a certain amount of the clinacanthus nutans powder is accurately weighed, and an appropriate amount of water is added according to the material-liquid ratio; 5, the clinacanthus nutans powder is put into ultrasonic waves of which the ultrasonic conditions are regulated in advance for ultrasonic treatment, wherein the conditions are set as followed: the ultrasonic time is set for 10-50 min, the ultrasonic temperature is set at 30 DEG C-70 DEG C, the material-liquid ratio is set to be 1:20-1:60, and the ultrasonic power is set at 72-216 W; 6, the solution processed through the ultrasonic waves are centrifuged at 6000 r/min for 15 min, and a supernatant solution, that is, a clinacanthus nutans active substance extracting solution is obtained.
Description
Technical field
The invention belongs to the technical field of exploitation plant resources, a kind of particularly method utilizing ultrasound wave to extract flavone, polyphenol, triterpene and vitamin C four kinds of active substances from melancholy escapes grass.
Background technology
Melancholy grass of escaping is a kind of growth by the tall and big herbaceous plant in equatorial low altitude area sparse woods or in shrubbery in moist sandy soil, and it is distributed widely in south east asia, and the ground such as China Hainan, Guangdong, Guangxi and Yunnan have wild.Melancholy grass of escaping generally is used as medicine with Herb or blade, and sweet in the mouth, micro-hardship are cool in nature, enters liver, kidney channel, has clearing away heat-damp and promoting diuresis, inducing diuresis to remove edema, effects such as thin warp, dehumidifying, antitumor of invigorating blood circulation, and can be used to insecticide or venom.It is reported, grass of melancholy being escaped in areas such as Hainan Mt. Wu-zhi Shan eats as vegetable, also has and drinks as Folium Camelliae sinensis, and blade of grass sheet of melancholy being escaped in addition is squeezed the juice and drunk, but owing to mainly concentrating on minority area, fails to find word historical data.The escape Related product of grass of current melancholy mainly concentrates on Hainan, comprises melancholy escape careless tea and melancholy of grass meal, melancholy of escaping and to escape careless bud green leaf etc.
Melancholy effect of presenting of grass of escaping depends primarily on wherein containing abundant flavone glycoside, Polyphenols, triterpenes and vitamin C isoreactivity composition, and is that indispensable basic document is processed in the utilization carrying out Nutrition Work and food by these active component high efficiency extraction.Existing is all concentrate on directly to be processed into product by melancholy blade of grass of escaping about the escape process technology of grass of melancholy, rare about the escape research of careless active substance extractive technique of melancholy, extracts the bibliographical information of various active material while of more not have from melancholy escapes grass.It is utilize ultrasound wave to produce and transmit powerful energy that ultrasonic assistant extracts, give medium great acceleration, when the energy that granule interior accepts is enough to the constraint overcoming structure, solid particle (or aggregate) is broken (or depolymerization), thus impels the stripping of effective ingredient in cell.Ultrasonic assistant extracts can extract active substance efficiently, and both shortened extraction time, and significantly improved extraction degree again, effectively can keep the maximum activity of active substance, method is simply efficient.
Summary of the invention
The object of this invention is to provide a kind of method simultaneously extracting polyphenol, flavone, triterpene and vitamin C four kinds of active substances from melancholy escapes grass, make easy and simple to handle, extraction ratio is high, energy consumption is low, cost is low, the cycle is short, and can fully develop melancholy escape grass value.
Technical scheme of the present invention: adopt four kinds of active substances that ultrasonic assistant extraction melancholy is escaped in grass, this extracting solution may be used for the raw material of the functional foods such as further exploitation and composition health product, oral liquid, also can be used as the nutrition accessory of other food of processing, therefore significant to improving the escape added value of careless intensive processing of melancholy.Its main step is: raw material through cleaning, drain and dry, pulverize, sieve after, regulate material-water ratio, ultrasonic temperature, ultrasonic power and ultrasonic time, ultrasonic completing is placed in centrifuge tube centrifugal, gets supernatant and namely obtains melancholy and to escape the extracting solution of careless active substance.
The present invention specifically comprises following technical process:
1. raw material cleaned up, drain, dry, use ultra-fine grinding mill pulverizing, obtain melancholy after crossing 200 mesh sieves and to escape grass meal end;
2. melancholy is escaped after grass meal subpackage, fresh-keeping for subsequent use at being placed in 5 DEG C, refrigerator.
3. regulate the ultrasonic power of ultrasonic washing unit, ultrasonic temperature and ultrasonic time.
4. accurately take a certain amount of melancholy to escape grass meal end, and add suitable quantity of water by solid-liquid ratio.
5. grass meal of melancholy being escaped is last puts into the ultrasonic washing unit supersound process regulating ultrasound condition in advance.
6., by the solution centrifugal 15min under 6000r/min after ultrasonic, the supernatant obtained is melancholy and escapes careless active substance extracting solution.
Above-mentionedly utilize ultrasonic assistant to extract melancholy to escape in the technological process of four kinds of active substances in grass, ultrasonic time 10 ~ 50min, ultrasonic temperature 30 ~ 70 DEG C, solid-liquid ratio 1:20 ~ 1:60, ultrasonic power 72 ~ 216W.By cooled solution centrifugal 15min under 6000r/min, collect supernatant be melancholy escape grass active substance extracting solution.
Beneficial effect of the present invention: the present invention makes full use of ultrasonic high-efficiency ground and is extracted melancholy simultaneously and escapes polyphenol, flavone, triterpene and vitamin C four kinds of active substances in grass.The vibrational energy that ultrasound wave produces makes to produce circulation in cell peripheral and cell, thus improves cell wall and membrane passage, and strengthening extraction process, is conducive to the extraction of effective ingredient in cell.Thus extract active substance efficiently, both shorten extraction time, significantly improved extraction degree again, thus greatly reduce production cost.The extracting method of active substance provided by the invention is applicable to other plant extract aspect too, is the plant resource of an effective exploitation China's abundant, improves the practicable technical method that China's novel plant resources economy is worth.
Detailed description of the invention
With specific embodiment, technical scheme of the present invention is described below.
Embodiment 1
Raw material is cleaned up, drains, dry, use ultra-fine grinding mill pulverizing, obtain melancholy after crossing 200 mesh sieves and to escape grass meal end; The melancholy obtained is escaped grass meal end be placed in 5 DEG C, refrigerator at fresh-keeping for subsequent use.Regulate the ultrasonic power 156W of ultrasonic washing unit, ultrasonic temperature 45 DEG C and ultrasonic time 30min; Take 4.541g melancholy grass meal end of escaping and be placed in 500mL conical flask, by solid-liquid ratio 1:55(m/V) add 250.0mL tap water; Subsequently conical flask is put into the ultrasonic washing unit supersound process regulating ultrasound condition in advance; By the solution centrifugal 15min under 6000r/min after ultrasonic, the supernatant obtained is melancholy and escapes careless active substance extracting solution.Polyphenol yield 9.387mg/g in extracting solution, flavone yield 28.785mg/g, triterpene yield 23.665mg/g, vitamin C yield 0.216mg/g after measured.
Embodiment 2
Raw material is cleaned up, drains, dry, use ultra-fine grinding mill pulverizing, obtain melancholy after crossing 200 mesh sieves and to escape grass meal end; The melancholy obtained is escaped grass meal end be placed in 5 DEG C, refrigerator at fresh-keeping for subsequent use.Regulate the ultrasonic power 144W of ultrasonic washing unit, ultrasonic temperature 50 DEG C and ultrasonic time 30min; Take 5.0920g melancholy grass meal end of escaping and be placed in 100mL conical flask, by solid-liquid ratio 1: 10(m/V) add 50.0mL tap water; Subsequently conical flask is put into the ultrasonic washing unit supersound process regulating ultrasound condition in advance; By the solution centrifugal 15min under 6000r/min after ultrasonic, the supernatant obtained is melancholy and escapes careless active substance extracting solution.Polyphenol yield 4.720mg/g in extracting solution, flavone yield 23.130mg/g, triterpene yield 12.432mg/g, vitamin C yield 0.029mg/g after measured.
Embodiment 3
Raw material is cleaned up, drains, dry, use ultra-fine grinding mill pulverizing, obtain melancholy after crossing 200 mesh sieves and to escape grass meal end; The melancholy obtained is escaped grass meal end be placed in 5 DEG C, refrigerator at fresh-keeping for subsequent use.Regulate the ultrasonic power 132W of ultrasonic washing unit, ultrasonic temperature 55 DEG C and ultrasonic time 20min; Take 1.0236g melancholy grass meal end of escaping and be placed in 100mL conical flask, by solid-liquid ratio 1: 50(m/V) add 50.0mL tap water; Subsequently conical flask is put into the ultrasonic washing unit supersound process regulating ultrasound condition in advance; By the solution centrifugal 15min under 6000r/min after ultrasonic, the supernatant obtained is melancholy and escapes careless active substance extracting solution.Through measuring polyphenol yield 7.365mg/g, flavone yield 23.896mg/g, triterpene yield 17.929mg/g, vitamin C yield 0.147mg/g in extracting solution.
Claims (4)
1. utilize ultrasonic assistant to extract melancholy to escape the methods of polyphenol, flavone, triterpene and vitamin C four kinds of active substances in grass, it is characterized in that, specifically comprise the following steps:
Pretreatment: cleaned up by raw material, drain, dry, uses ultra-fine grinding mill pulverizing, obtains melancholy and escape grass meal end after crossing 200 mesh sieves; After subpackage, fresh-keeping for subsequent use at being placed in 5 DEG C, refrigerator;
Regulate the technological parameter of ultrasonic washing unit: ultrasonic power, ultrasonic temperature and ultrasonic time;
Weigh: by material-water ratio weigh appropriate melancholy escape grass meal end and distilled water in conical flask;
Supersound extraction: put into ultrasonic washing unit supersound process by being equipped with the escape conical flask at grass meal end of melancholy;
Centrifugal: the rotating speed of centrifuge is 6000r/min, and centrifugation time is 15min.
2. the ultrasound assisted extraction melancholy that utilizes according to claim 1 is escaped the method for active substance in grass, it is characterized in that: melancholy at the process conditions can be calculated according to gained extracting solution and to escape polyphenol yield 4.720 ~ 9.641mg/g in grass, flavone yield 21.227 ~ 28.896mg/g, triterpene yield 11.849 ~ 23.665mg/g, vitamin C yield 0.025 ~ 0.837mg/g.
3. the ultrasound assisted extraction melancholy that utilizes according to claim 1 is escaped the method for active substance in grass, and it is characterized in that: ultrasonic power is 72 ~ 216W, ultrasonic temperature is 30 ~ 70 DEG C, and ultrasonic time is 10 ~ 50min.
4. the ultrasound assisted extraction melancholy that utilizes according to claim 1 is escaped the method for active substance in grass, it is characterized in that: material-water ratio is 1:20 ~ 1:60.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510722739.8A CN105326873A (en) | 2015-10-31 | 2015-10-31 | Method for extracting clinacanthus nutans active substances through ultrasonic assistance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510722739.8A CN105326873A (en) | 2015-10-31 | 2015-10-31 | Method for extracting clinacanthus nutans active substances through ultrasonic assistance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105326873A true CN105326873A (en) | 2016-02-17 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510722739.8A Pending CN105326873A (en) | 2015-10-31 | 2015-10-31 | Method for extracting clinacanthus nutans active substances through ultrasonic assistance |
Country Status (1)
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| CN (1) | CN105326873A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954765A (en) * | 2017-04-11 | 2017-07-18 | 广东人为峰健康管理有限公司 | A kind of insect feedstuff of green safety and its preparation method and application |
| CN109824658A (en) * | 2019-04-02 | 2019-05-31 | 聊城大学 | A method of 3 kinds of flavonoid glycosides are purified from sorrow extracting and developing in grass of escaping |
| CN114989248A (en) * | 2022-04-28 | 2022-09-02 | 福州大学 | Clinacanthus nutans polypeptide with anti-inflammatory and antioxidant activities as well as preparation method and application thereof |
-
2015
- 2015-10-31 CN CN201510722739.8A patent/CN105326873A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 张卫华 等: ""鳄嘴花总黄酮超声提取工艺优化及其体外抗氧化活性考察"", 《中国实验方剂学杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954765A (en) * | 2017-04-11 | 2017-07-18 | 广东人为峰健康管理有限公司 | A kind of insect feedstuff of green safety and its preparation method and application |
| CN109824658A (en) * | 2019-04-02 | 2019-05-31 | 聊城大学 | A method of 3 kinds of flavonoid glycosides are purified from sorrow extracting and developing in grass of escaping |
| CN114989248A (en) * | 2022-04-28 | 2022-09-02 | 福州大学 | Clinacanthus nutans polypeptide with anti-inflammatory and antioxidant activities as well as preparation method and application thereof |
| CN114989248B (en) * | 2022-04-28 | 2023-11-21 | 福州大学 | Clinopodium polycephalum polypeptide with anti-inflammatory and antioxidant activities, and preparation method and application thereof |
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