CN105326055A - Greengage bittern essence functional food containing rich phenolic substances and organic acid and preparation method of greengage bittern essence functional food - Google Patents
Greengage bittern essence functional food containing rich phenolic substances and organic acid and preparation method of greengage bittern essence functional food Download PDFInfo
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- 150000007524 organic acids Chemical class 0.000 title claims abstract description 82
- 235000013376 functional food Nutrition 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 title claims abstract description 17
- 239000000126 substance Substances 0.000 title abstract description 9
- 241001131796 Botaurus stellaris Species 0.000 title abstract 7
- 235000011230 Prunus domestica subsp. italica Nutrition 0.000 title abstract 7
- 244000249693 Reneklode Species 0.000 title abstract 7
- 239000000284 extract Substances 0.000 claims abstract description 71
- 229910052736 halogen Inorganic materials 0.000 claims description 78
- 150000002367 halogens Chemical class 0.000 claims description 78
- 150000001299 aldehydes Chemical class 0.000 claims description 74
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 33
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 32
- 239000011347 resin Substances 0.000 claims description 28
- 229920005989 resin Polymers 0.000 claims description 28
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 20
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- 239000002253 acid Substances 0.000 claims description 14
- 238000005352 clarification Methods 0.000 claims description 13
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- 239000002250 absorbent Substances 0.000 claims description 12
- 238000010521 absorption reaction Methods 0.000 claims description 12
- 239000003480 eluent Substances 0.000 claims description 12
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 claims description 12
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- 229940074391 gallic acid Drugs 0.000 claims description 6
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 4
- 150000001298 alcohols Chemical class 0.000 claims description 4
- 235000019253 formic acid Nutrition 0.000 claims description 4
- 229920006395 saturated elastomer Polymers 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 3
- 238000002479 acid--base titration Methods 0.000 claims description 2
- 238000010828 elution Methods 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims description 2
- 239000000706 filtrate Substances 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 12
- 230000008569 process Effects 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000004134 energy conservation Methods 0.000 abstract 1
- 238000005554 pickling Methods 0.000 abstract 1
- 235000021018 plums Nutrition 0.000 abstract 1
- 238000011010 flushing procedure Methods 0.000 description 10
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- 238000011084 recovery Methods 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 150000003839 salts Chemical class 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- NGSWKAQJJWESNS-UHFFFAOYSA-N 4-coumaric acid Chemical compound OC(=O)C=CC1=CC=C(O)C=C1 NGSWKAQJJWESNS-UHFFFAOYSA-N 0.000 description 4
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 4
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- 238000004128 high performance liquid chromatography Methods 0.000 description 4
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- 238000002156 mixing Methods 0.000 description 4
- 229920001778 nylon Polymers 0.000 description 4
- 235000021110 pickles Nutrition 0.000 description 4
- 238000005086 pumping Methods 0.000 description 4
- 238000010612 desalination reaction Methods 0.000 description 3
- 238000005265 energy consumption Methods 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 2
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 2
- NGSWKAQJJWESNS-ZZXKWVIFSA-M 4-Hydroxycinnamate Natural products OC1=CC=C(\C=C\C([O-])=O)C=C1 NGSWKAQJJWESNS-ZZXKWVIFSA-M 0.000 description 2
- GYFFKZTYYAFCTR-JUHZACGLSA-N 4-O-trans-caffeoylquinic acid Chemical compound O[C@@H]1C[C@](O)(C(O)=O)C[C@@H](O)[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 GYFFKZTYYAFCTR-JUHZACGLSA-N 0.000 description 2
- GYFFKZTYYAFCTR-UHFFFAOYSA-N 5-O-(6'-O-galloyl)-beta-D-glucopyranosylgentisic acid Natural products OC1CC(O)(C(O)=O)CC(O)C1OC(=O)C=CC1=CC=C(O)C(O)=C1 GYFFKZTYYAFCTR-UHFFFAOYSA-N 0.000 description 2
- DFYRUELUNQRZTB-UHFFFAOYSA-N Acetovanillone Natural products COC1=CC(C(C)=O)=CC=C1O DFYRUELUNQRZTB-UHFFFAOYSA-N 0.000 description 2
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
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- 210000004369 blood Anatomy 0.000 description 2
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 2
- 229940074393 chlorogenic acid Drugs 0.000 description 2
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 2
- 235000001368 chlorogenic acid Nutrition 0.000 description 2
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 2
- GYFFKZTYYAFCTR-LMRQPLJMSA-N cryptochlorogenic acid Natural products O[C@H]1C[C@@](O)(C[C@H](O)[C@H]1OC(=O)C=Cc2ccc(O)c(O)c2)C(=O)O GYFFKZTYYAFCTR-LMRQPLJMSA-N 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
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- 150000002632 lipids Chemical class 0.000 description 2
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- 235000011090 malic acid Nutrition 0.000 description 2
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- 235000009048 phenolic acids Nutrition 0.000 description 2
- GWTUHAXUUFROTF-UHFFFAOYSA-N pseudochlorogenic acid Natural products C1C(O)C(O)C(O)CC1(C(O)=O)OC(=O)C=CC1=CC=C(O)C(O)=C1 GWTUHAXUUFROTF-UHFFFAOYSA-N 0.000 description 2
- 230000001603 reducing effect Effects 0.000 description 2
- CWVRJTMFETXNAD-NXLLHMKUSA-N trans-5-O-caffeoyl-D-quinic acid Chemical compound O[C@H]1[C@H](O)C[C@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-NXLLHMKUSA-N 0.000 description 2
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- 239000002351 wastewater Substances 0.000 description 1
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- Preparation Of Fruits And Vegetables (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of greengage bittern essence functional food containing rich phenolic substances and organic acid. Greengage bittern produced in the pickling processing process of green plums is taken as a raw material, and phenolic substance components and organic acid components are separated and recycled respectively. The invention further discloses the greengage bittern essence functional food containing rich phenolic substances and organic acid. The greengage bittern essence functional food comprises extracts of the rich phenolic substances and extracts of the rich organic acid, wherein the mass percentage of the extracts of the rich phenolic substances is 5%-30%, and the mass percentage of the extracts of the rich organic acid is 70%-95%. By means of the method, greengage bittern resources can be effectively utilized, energy conservation and environmental protection are achieved, and a product prepared with the method has high added value.
Description
Technical field
The invention belongs to technical field of functional food, be specifically related to one and be rich in aldehydes matter and organic acid plum halogen essence functional food and preparation method thereof.
Background technology
Green plum (PrunusmumeSieb.etZucc.) is also called smoked plum, fruit plum, for rose family apricot belongs to the fruit of arbor, mainly be distributed in the Yangtze river basin and south China, southwest one band, more with the output that Guangdong, Guangxi, Yunnan, Fujian, Zhejiang, Jiangsu etc. are economized.Recent study shows, green plum has anti-oxidant, anti-inflammatory, antibacterial, hypoglycemic, reducing blood lipid, anti-trioxypurine, fat-reducing and adjustment gastrointestinal function isoreactivity, has wide exploitation prospect.Green plum, as a kind of fruit of medicine-food two-purpose, because acidity is too high and should not eat raw, is a kind of typical processing type fruit.For a long time, except part is made into except Chinese medicine dark plum, green plum is mainly used in the processing of preserved fruit, first pickles with salt after fruit picking, makes plum base, and then carries out following process.
Pickle at green plum and produce in plum base process, every 1000kg green plum fresh fruit adds the salt of 250 ~ 300kg, and produce the salt marsh liquid of about 400kg, this salt marsh liquid is plum halogen.Due to current green plum be all mainly pickle after be reprocessed into the products such as preserved fruit, by China's green plum output 400,000 tons calculating, about produce plum halogen 160,000 tons.In China's green plum secondary industry, plum halogen is not originally utilized, and the overwhelming majority is discharged directly in environment, both wastes resource, and pollutes environment again.
According to analysis, containing the salt of about 15% ~ 20% in plum halogen, up to 120,000mg/L (Kubo etc., 2001), (Huang Demin reports the COD of plum halogen to its COD (COD)
crbe 10,000mg/L), pH is less than 2.5, if discharged directly in environment, is typical high salt, peracid, high-COD waste water.Because plum halogen contains a large amount of salt and organic acid, comparatively large to the destructive power of Environment-Ecosystem, make local environment salinization and acidifying, break the local ecological balance.Develop the effect that plum halogen can play protection of the environment.
Plum halogen is the mixture of fruit plum juice and salt in fact, and than water, main nutrition and the bioactivator such as organic acid, carbohydrate, phenols containing leaching from fruit plum, generally not containing noxious material, is the resource of preciousness.Develop plum halogen and can play the object economized on resources.
Plum halogen is not also utilized to process the report of essence functional food at present.Patent 200710033012.4 discloses a kind of method for making of plum halogen beverage, plum halogen after filtration, concentrating and desalinating, clarification, technique such as essence filter, allotment etc., obtain a kind of plum halogen health drink; Patent 201010227224.8 discloses a kind of method of producing plum juice via plum halogen desalination, and plum halogen, after normal pressure distillation and concentration, extracts desalination with edible alcohol, then recovered alcohol, and the liquid obtained when concentrate and distillation mixes, thus obtains desalination plum juice.These two patents all need to carry out concentration, not only energy consumption is high, and the bioactive substance content contained in the product obtained is low, therefore, the plum halogen essence functional food production technology developing biologically active content in a kind of low energy consumption, product high is very necessary.
Summary of the invention
First technical problem to be solved by this invention is to provide rich a kind of preparation method containing aldehydes matter and organic acid plum halogen essence functional food, this preparation method's energy consumption and cost low, environmental friendliness, and obtain plum halogen essence functional food in aldehydes matter and organic acid content high.
Second technical problem to be solved by this invention is to provide one and is rich in aldehydes matter and organic acid plum halogen essence functional food, this functional food adopts and comprises the extract being rich in aldehydes matter that above-mentioned preparation method obtains and be rich in organic acid extract as active component, this functional food is rich in aldehydes matter and organic acid, and added value of product is high.
First technical problem to be solved by this invention is achieved through the following technical solutions: a kind of preparation method being rich in aldehydes matter and organic acid plum halogen essence functional food, comprises the following steps:
(1) get plum halogen, it is centrifugal to get filtrate after filtration, obtains clarification plum halogen, clarification plum halogen is joined in large pore resin absorption column, collects omission timber 1 for subsequent use, detect aldehydes matter in omission timber 1, being positive, it is saturated to show that large pore resin absorption column has adsorbed, and stops adding set-in of plum rains halogen;
(2) with purified rinse water large pore resin absorption column to eluate water white transparency, adopt acid alcohol solvent elution large pore resin absorption column, collect eluent and concentrate be rich in the extract of aldehydes matter;
(3) get the omission timber 1 in step (1), join in anion-exchange resin column, collect omission timber 2, detect the organic acid in omission timber 2, present the positive and show that anion-exchange resin column absorption is saturated, stop adding omission timber 1;
(4) use purified rinse water anion-exchange resin column to eluate water white transparency, adopt alcohols solvent wash-out, collect eluent so concentrated that to be rich in organic acid extract.
Be rich in the preparation method of aldehydes matter and organic acid plum halogen essence functional food above-mentioned:
When filtering in step (1), the aperture of filter cloth is preferably 80 ~ 300 orders, and time centrifugal, the rotating speed of centrifuge is preferably 4000 ~ 10000 revs/min.
Large pore resin absorption column described in step (1) is preferably X-5 macroporous absorbent resin or AB-8 macroporous absorbent resin.
Forint-phenol law is preferably adopted to detect aldehydes matter in omission timber 1 in step (1).
Acid alcohol solvent described in step (2) is the alcohol solution containing acid, wherein said acid is hydrochloric acid or formic acid, described alcohol solution is methanol aqueous solution or ethanol water, wherein the volumn concentration of methyl alcohol or ethanol is 30 ~ 95%, and the volumn concentration of hydrochloric acid or formic acid is 0.08 ~ 0.12%.
The total phenol content in the extract of aldehydes matter that is rich in described in step (2) is not less than 20.0%, weight ratio, in gallic acid.
Detect through high performance liquid chromatography, the extract being rich in aldehydes matter of the present invention mainly contains the phenolic acids such as neochlorogenic acid, chlorogenic acid, Cryptochlorogenic acid and p-Coumaric Acid.
Anion-exchange resin column described in step (3) is preferably 717 type anion exchange resin or D301G type anion exchange resin.
The organic acid preferably adopting pH meter or acid-base titration to detect in omission timber 2 in step (3) is positive.
Alcohols solvent described in step (4) is methanol aqueous solution or ethanol water, its volumn concentration 30 ~ 80%; The total acid content in organic acid extract that is rich in described in step (4) is not less than 30%, weight ratio, with citrometer.
Detect through high performance liquid chromatography, the organic acid extract that is rich in of the present invention mainly contains citric acid and malic acid.
Second technical problem to be solved by this invention is achieved through the following technical solutions: one is rich in aldehydes matter and organic acid plum halogen essence functional food, comprise and adopt the extract being rich in aldehydes matter that obtains of said method and be rich in organic acid extract, the mass percentage being wherein rich in the extract of aldehydes matter is 5% ~ 30%, and the mass percentage being rich in organic acid extract is 70% ~ 95%.
Namely the present invention pickles the plum halogen that produces in process for raw material with green plum, separate and recover Phenolic components wherein and organic acid composition, after two kinds of components are mixed by a certain percentage (also can add the auxiliary material of this area routine as required), be prepared into said formulation in function bromatology according to a conventional method, obtain and be of the present inventionly rich in aldehydes matter and organic acid plum halogen essence functional food.
Compared with prior art, tool of the present invention has the following advantages:
(1) cost is low: the accessory substance plum halogen that processing pickled by current green plum is abandoned as discarded object, also be not used, the present invention with plum halogen for raw material, greatly reduce production cost, improve the utilization rate of resource, in addition, the solvent that product of the present invention uses in preparation process through reclaim, reusable edible;
(2) environmental friendliness: the present invention has carried out deep processing to the accessory substance plum halogen that processing pickled by green plum, turn waste into wealth, decrease the discharge to environmentally hazardous substance, environmental friendliness, recycle eluting solvent in whole production process, do not bring new pollution to environment;
(3) added value of product is high: be rich in aldehydes matter and the organic acid plum halogen essence functional food that utilize plum halogen to develop have anti-oxidant, antibacterial, reducing blood lipid, anti-trioxypurine, the function such as aid digestion, have more wide market prospects, the added value brought is higher.
Accompanying drawing explanation
Fig. 1 is the flow chart being rich in the preparation method of aldehydes matter and organic acid plum halogen essence functional food in embodiment of the present invention 1-4.
Detailed description of the invention
Following examples only for setting forth the present invention, do not limit the scope of the invention.Those skilled in the art, according to above scope of disclosure, all can realize object of the present invention.
Embodiment 1
As shown in fig. 1, the preparation method of what the present embodiment provided be rich in aldehydes matter and organic acid plum halogen essence functional food, comprises the following steps:
(1) get plum halogen 100L, 100 order nylon filtering cloths filter, and 10000 revs/min centrifugal, obtain clarification plum halogen (wherein filtered fluid dilution discharge or recovery sodium chloride); Clarification plum halogen is pumped into (this preprocess method is provided by production of resins company through pretreatment, mainly high concentration ethanol soak, sodium hydroxide solution and watery hydrochloric acid flushing etc.) X-5 macroporous absorbent resin, collect omission timber 1 for subsequent use, and the aldehydes matter detected in omission timber 1, adopt forint-phenol law to detect aldehydes matter in omission timber 1, stop adding set-in of plum rains halogen when presenting positive reaction;
(2) clarify to flushing liquor with purified rinse water resin column, with 80% (volumn concentration) ethanol water wash-out containing 0.1% hydrochloric acid (volumn concentration), collect eluent, concentration and recovery ethanol, obtain the extract being rich in aldehydes matter, the total phenol content being rich in the extract of aldehydes matter obtained is not less than 20.0%, weight ratio, in gallic acid;
Detect through high performance liquid chromatography, the extract being rich in aldehydes matter of the present invention mainly contains the phenolic acids such as neochlorogenic acid, chlorogenic acid, Cryptochlorogenic acid and p-Coumaric Acid.
(3) omission timber 1 through X-5 macroporous absorbent resin absorbing phenolic material is pumped into through pretreatment (company provides by production of resins, mainly high concentration ethanol soak, sodium hydroxide solution and watery hydrochloric acid flushing etc.) 717 anion exchange resin, collect omission timber 2, detect the organic acid in omission timber 2, stop pumping into when presenting positive reaction;
(4) clarify to flushing liquor with purified rinse water 717 anion-exchange resin column, with the ethanol water wash-out that volumn concentration is 30%, collect eluent, concentration and recovery ethanol, obtain being rich in the extracts such as organic acid, the obtained total acid content in organic acid extract that is rich in is not less than 30%, weight ratio, with citrometer;
Detect through high performance liquid chromatography, the organic acid extract that is rich in of the present invention mainly contains citric acid and malic acid.
What adopt the above-mentioned obtained extract being rich in aldehydes matter and be rich in that organic acid extract makes as active component is rich in aldehydes matter and organic acid plum halogen essence functional food, the mass percentage being wherein rich in the extract of aldehydes matter is 5%, and the mass percentage being rich in organic acid extract is 95%.
Preparation process is: two kinds of extracts are carried out freeze drying, obtain respective powder, be 5% by the mass percentage of the extract being rich in aldehydes matter, the mass percentage being rich in organic acid extract is 95% mixing, incapsulate, aldehydes matter and organic acid plum halogen essence functional food must be rich in.
Embodiment 2
As shown in fig. 1, the preparation method of what the present embodiment provided be rich in aldehydes matter and organic acid plum halogen essence functional food, comprises the following steps:
(1) get plum halogen 100L, 300 order nylon filtering cloths filter, and 8000 revs/min centrifugal, obtain clarification plum halogen; Clarification plum halogen is pumped into through pretreated AB-8 macroporous absorbent resin, collect omission timber 1 for subsequent use, and detect the aldehydes matter in omission timber 1, stop adding set-in of plum rains halogen when presenting positive reaction;
(2) clarify to flushing liquor with purified rinse water resin column, with 50% (volumn concentration) ethanol water wash-out containing 0.1% hydrochloric acid (volumn concentration), collect eluent, concentration and recovery ethanol, obtain the extract being rich in aldehydes matter, the total phenol content being rich in the extract of aldehydes matter obtained is not less than 20.0%, weight ratio, in gallic acid;
(3) omission timber 1 through AB-8 macroporous absorbent resin absorbing phenolic material is pumped into through pretreated 717 anion exchange resin, collect omission timber 2, detect the organic acid in omission timber 2, stop pumping into when presenting positive reaction;
(4) clarify to flushing liquor with purified rinse water D301G anion exchange resin, with the ethanol water wash-out that volumn concentration is 50%, collect eluent, concentration and recovery ethanol, obtain being rich in the extracts such as organic acid, the obtained total acid content in organic acid extract that is rich in is not less than 30%, weight ratio, with citrometer;
What adopt the above-mentioned obtained extract being rich in aldehydes matter and be rich in that organic acid extract makes as active component is rich in aldehydes matter and organic acid plum halogen essence functional food, the mass percentage being wherein rich in the extract of aldehydes matter is 10%, and the mass percentage being rich in organic acid extract is 90%.
Preparation process is: two kinds of extracts are carried out freeze drying, obtain respective powder, be 10% by the mass percentage of the extract being rich in aldehydes matter, the mass percentage being rich in organic acid extract is 90% mixing, incapsulate, aldehydes matter and organic acid plum halogen essence functional food must be rich in.
Embodiment 3
As shown in fig. 1, the preparation method of what the present embodiment provided be rich in aldehydes matter and organic acid plum halogen essence functional food, comprises the following steps:
(1) get plum halogen 100L, 80 order nylon filtering cloths filter, and 6000 revs/min centrifugal, obtain clarification plum halogen; Clarification plum halogen is pumped into through pretreated X-5 macroporous absorbent resin, collect omission timber 1 for subsequent use, and detect the aldehydes matter in omission timber 1, stop adding set-in of plum rains halogen when presenting positive reaction;
(2) clarify to flushing liquor with purified rinse water resin column, with 30% (volumn concentration) methanol aqueous solution wash-out containing 0.12% hydrochloric acid (volumn concentration), collect eluent, concentration and recovery methyl alcohol, obtain the extract being rich in aldehydes matter, the total phenol content being rich in the extract of aldehydes matter obtained is not less than 20.0%, weight ratio, in gallic acid;
(3) omission timber 1 through X-5 macroporous absorbent resin absorbing phenolic material is pumped into through pretreated 717 anion exchange resin, collect omission timber 2, detect the organic acid in omission timber 2, stop pumping into when presenting positive reaction;
(4) clarify to flushing liquor with purified rinse water D301G anion exchange resin, with the methanol aqueous solution wash-out that volumn concentration is 30%, collect eluent, concentration and recovery methyl alcohol, obtain being rich in the extracts such as organic acid, the obtained total acid content in organic acid extract that is rich in is not less than 30%, weight ratio, with citrometer;
What adopt the above-mentioned obtained extract being rich in aldehydes matter and be rich in that organic acid extract makes as active component is rich in aldehydes matter and organic acid plum halogen essence functional food, the mass percentage being wherein rich in the extract of aldehydes matter is 30%, and the mass percentage being rich in organic acid extract is 70%.
Preparation process is: two kinds of extracts are carried out freeze drying, obtain respective powder, be 30% by the mass percentage of the extract being rich in aldehydes matter, the mass percentage being rich in organic acid extract is 70% mixing, add tablet and commonly use auxiliary material, be pressed into tablet, aldehydes matter and organic acid plum halogen essence functional food must be rich in.
Embodiment 4
As shown in fig. 1, the preparation method of what the present embodiment provided be rich in aldehydes matter and organic acid plum halogen essence functional food, comprises the following steps:
(1) get plum halogen 100L, 200 order nylon filtering cloths filter, and 4000 revs/min centrifugal, obtain clarification plum halogen; Clarification plum halogen is pumped into through pretreated X-5 macroporous absorbent resin, collect omission timber 1 for subsequent use, and detect the aldehydes matter in omission timber 1, stop adding set-in of plum rains halogen when presenting positive reaction;
(2) clarify to flushing liquor with purified rinse water resin column, with 92% (volumn concentration) ethanol water wash-out containing 0.08% hydrochloric acid (volumn concentration), collect eluent, concentration and recovery ethanol, obtain the extract being rich in aldehydes matter, the total phenol content being rich in the extract of aldehydes matter obtained is not less than 20.0%, weight ratio, in gallic acid;
(3) omission timber 1 through X-5 macroporous absorbent resin absorbing phenolic material is pumped into through pretreated 717 anion exchange resin, collect omission timber 2, detect the organic acid in omission timber 2, stop pumping into when presenting positive reaction;
(4) clarify to flushing liquor with purified rinse water D301G anion exchange resin, with the ethanol water wash-out that volumn concentration is 80%, collect eluent, concentration and recovery ethanol, obtain being rich in the extracts such as organic acid, the obtained total acid content in organic acid extract that is rich in is not less than 30%, weight ratio, with citrometer;
What adopt the above-mentioned obtained extract being rich in aldehydes matter and be rich in that organic acid extract makes as active component is rich in aldehydes matter and organic acid plum halogen essence functional food, the mass percentage being wherein rich in the extract of aldehydes matter is 20%, and the mass percentage being rich in organic acid extract is 80%.
Preparation process is: two kinds of extract pure water constant volumes are become equal volume, be 20% by the mass percentage of the extract being rich in aldehydes matter, the mass percentage being rich in organic acid extract is 80% mixing, make oral liquid, aldehydes matter and organic acid plum halogen essence functional food must be rich in.
Claims (10)
1. be rich in a preparation method for aldehydes matter and organic acid plum halogen essence functional food, it is characterized in that comprising the following steps:
(1) get plum halogen, it is centrifugal to get filtrate after filtration, obtains clarification plum halogen, clarification plum halogen is joined in large pore resin absorption column, collects omission timber 1 for subsequent use, detect aldehydes matter in omission timber 1, being positive, it is saturated to show that large pore resin absorption column has adsorbed, and stops adding set-in of plum rains halogen;
(2) with purified rinse water large pore resin absorption column to eluate water white transparency, adopt acid alcohol solvent elution large pore resin absorption column, collect eluent and concentrate be rich in the extract of aldehydes matter;
(3) get the omission timber 1 in step (1), join in anion-exchange resin column, collect omission timber 2, detect the organic acid in omission timber 2, present the positive and show that anion-exchange resin column absorption is saturated, stop adding omission timber 1;
(4) use purified rinse water anion-exchange resin column to eluate water white transparency, adopt alcohols solvent wash-out, collect eluent so concentrated that to be rich in organic acid extract.
2. the preparation method being rich in aldehydes matter and organic acid plum halogen essence functional food according to claim 1, it is characterized in that: when filtering in step (1), the aperture of filter cloth is 80 ~ 300 orders, and time centrifugal, the rotating speed of centrifuge is 4000 ~ 10000 revs/min.
3. the preparation method being rich in aldehydes matter and organic acid plum halogen essence functional food according to claim 1, is characterized in that: the large pore resin absorption column described in step (1) is X-5 macroporous absorbent resin or AB-8 macroporous absorbent resin.
4. the preparation method being rich in aldehydes matter and organic acid plum halogen essence functional food according to claim 1, is characterized in that: adopt forint-phenol law to detect aldehydes matter in omission timber 1 in step (1).
5. the preparation method being rich in aldehydes matter and organic acid plum halogen essence functional food according to claim 1, it is characterized in that: the acid alcohol solvent described in step (2) is the alcohol solution containing acid, wherein said acid is hydrochloric acid or formic acid, described alcohol solution is methanol aqueous solution or ethanol water, wherein the volumn concentration of methyl alcohol or ethanol is 30 ~ 95%, and the volumn concentration of hydrochloric acid or formic acid is 0.08 ~ 0.12%.
6. the preparation method being rich in aldehydes matter and organic acid plum halogen essence functional food according to claim 1, it is characterized in that: the total phenol content in the extract of aldehydes matter that is rich in described in step (2) is not less than 20.0%, weight ratio, in gallic acid.
7. the preparation method being rich in aldehydes matter and organic acid plum halogen essence functional food according to claim 1, is characterized in that: the anion-exchange resin column described in step (3) is 717 type anion exchange resin or D301G type anion exchange resin.
8. the preparation method being rich in aldehydes matter and organic acid plum halogen essence functional food according to claim 1, is characterized in that: adopt the organic acid in pH meter or acid-base titration detection omission timber 2 in step (3).
9. the preparation method being rich in aldehydes matter and organic acid plum halogen essence functional food according to claim 1, it is characterized in that: the alcohols solvent described in step (4) is methanol aqueous solution or ethanol water, the volumn concentration 30 ~ 80% of described methyl alcohol or ethanol; The total acid content in organic acid extract that is rich in described in step (4) is not less than 30%, weight ratio, with citrometer.
10. one kind is rich in aldehydes matter and organic acid plum halogen essence functional food, comprise and adopt the extract being rich in aldehydes matter obtained any one of claim 1-9 and be rich in organic acid extract, the mass percentage being wherein rich in the extract of aldehydes matter is 5% ~ 30%, and the mass percentage being rich in organic acid extract is 70% ~ 95%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109953312A (en) * | 2019-03-06 | 2019-07-02 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of plum halogen red acid soup and preparation method thereof |
| CN111978422A (en) * | 2020-08-25 | 2020-11-24 | 湖南华诚生物资源股份有限公司 | Method for separating multiple active ingredients from waste liquid after pigment extraction from roselle |
| CN112544764A (en) * | 2020-11-19 | 2021-03-26 | 溜溜果园集团股份有限公司 | Acid locking process in green plum product processing process |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5261254A (en) * | 1975-11-15 | 1977-05-20 | Kiyomatsu Moriyama | Method of pickling plum with salt |
| CN101214074A (en) * | 2007-12-29 | 2008-07-09 | 广东省农业科学院蚕业与农产品加工研究所 | Greengage bittern health care beverage and preparation thereof |
| CN101904528A (en) * | 2010-07-15 | 2010-12-08 | 大理学院 | Method for producing plum juice via plum halogen desalination |
| CN104642701A (en) * | 2015-03-11 | 2015-05-27 | 广东殿羽田食品有限公司 | Preparation method of organic acid-rich green plum preserved fruit |
-
2015
- 2015-11-23 CN CN201510821422.XA patent/CN105326055B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5261254A (en) * | 1975-11-15 | 1977-05-20 | Kiyomatsu Moriyama | Method of pickling plum with salt |
| CN101214074A (en) * | 2007-12-29 | 2008-07-09 | 广东省农业科学院蚕业与农产品加工研究所 | Greengage bittern health care beverage and preparation thereof |
| CN101904528A (en) * | 2010-07-15 | 2010-12-08 | 大理学院 | Method for producing plum juice via plum halogen desalination |
| CN104642701A (en) * | 2015-03-11 | 2015-05-27 | 广东殿羽田食品有限公司 | Preparation method of organic acid-rich green plum preserved fruit |
Non-Patent Citations (2)
| Title |
|---|
| 赖来展: "《黑色食品加工工艺与配方》", 30 April 2001, 科学技术文献出版社 * |
| 陈智毅等: "青梅加工副产物的成分分析", 《食品与机械》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109953312A (en) * | 2019-03-06 | 2019-07-02 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of plum halogen red acid soup and preparation method thereof |
| CN111978422A (en) * | 2020-08-25 | 2020-11-24 | 湖南华诚生物资源股份有限公司 | Method for separating multiple active ingredients from waste liquid after pigment extraction from roselle |
| CN112544764A (en) * | 2020-11-19 | 2021-03-26 | 溜溜果园集团股份有限公司 | Acid locking process in green plum product processing process |
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Denomination of invention: A plum bittern essence functional food rich in phenols and organic acids and its preparation method Granted publication date: 20170822 Pledgee: Bank of Communications Co.,Ltd. Jieyang Branch Pledgor: Puning xiaomeiwu Food Co.,Ltd. Registration number: Y2024980006836 |