CN105325297B - The seedling counting method of Seeds of Dendrobium Candidum - Google Patents
The seedling counting method of Seeds of Dendrobium Candidum Download PDFInfo
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- CN105325297B CN105325297B CN201510781009.5A CN201510781009A CN105325297B CN 105325297 B CN105325297 B CN 105325297B CN 201510781009 A CN201510781009 A CN 201510781009A CN 105325297 B CN105325297 B CN 105325297B
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- seed
- seedling
- dendrobium candidum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention discloses a kind of seedling counting methods of Seeds of Dendrobium Candidum, and by the seed germination and growth of dendrobium candidum at seedling, the quantity by calculating seedling obtains the quantity of seed.Since the genealogy of law broadcasts seed that is subtle, being difficult in the dendrobium candidum capsule that using routine, direct discernable by eye counts in media surface, the nutrition abundant using culture medium is prepared, it is sprouted for Seeds of Dendrobium Candidum and growth supplies nutrients and moisture, ensure that the seed that its capsule preserves can survive substantially on culture medium, and allow seed germination and growth that can understand a certain size the seedling differentiated and counted at naked eyes, make it intuitive, easy counting, then can be obtained the quantity of seed by counting seedling quantity.The present invention using it is easy, highly practical, need not be by other more accurate microsurgical instrument equipment, seed amount that can be in accurate counting dendrobium candidum capsule, to conveniently obtain the quantity of production seedling, this provides accurately guidance and reference for extensive nursery and production.
Description
Technical field
The invention belongs to the subtle seed quick counter technical field of plant more particularly to a kind of seedlings of Seeds of Dendrobium Candidum
Counting method.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo), orchid family Dendrobium perennial herb are planted
Object, conventional Chinese medicine are《Pharmacopoeia of People's Republic of China》It is recorded.Be distributed in China Qinling Mountains, on the south Huaihe River all provinces and regions, be grown in
On lofty mountains and steep hills overhanging cliff and rock crevice or remote mountains ancient tree.Dendrobium candidum have promote the production of body fluid, quench the thirst, easing pain, eliminate oedema it
Effect cures mainly the diseases such as the pyreticosis deficiency of Yin, mesh are dark, stomach is weak, hoarseness, and to vocal cords fatigue, hoarseness, restoring its U.S. sound has spy
Different curative effect.Dendrobium candidum is endangered species, belongs to country-level national key protected plant, the iron sheet maple after fresh stem and frying drying
Bucket is all rare traditional Chinese medicine.Modern clinic is applied and pharmacological research confirms, dendrobium candidum has nourishing Yin and moistening lung, nourishing the stomach to improve the production of body fluid, is good for
Brain improving eyesight, liver protection calming heart, heat-clearing and fire-reducing, the consumptive disease of tonifying five zang organs, macrobiosis of making light of one's life by commiting suicide magical effect, to chronic fatigue syndrome, lung
Disease, coronary heart disease, nephrosis, chronic liver disease, hypertension, hyperlipidemia, hyperglycemia, diabetes, chronic gastropathy, dry syndrome, cancer
Etc. diseases have definite curative effect and conditioning rehabilitative action；Enhance body immunity, anti-oxidant, anti-aging, to excessive drinking and smoking, fatigue
Excessively, night life is excessively, with eye is overstrain one's nerves, hoarseness, mouth parched and tongue scorched, the weak power of old age decline, that the rehabilitations such as the deficiency of Yin have is special
Curative effect.
For germination percentage not as good as 5%, breeding potential is very low in its natural state for dendrobium candidum, is mostly bred with offshoot, it is difficult to full
The sufficient market demand.Present dendrobium candidum mainly using seed as explant, carry out tissue cultures production test tube seedling mode carry out it is numerous
It educates.Seeds of Dendrobium Candidum is primarily present in capsule, and capsule is about 1.8~2.2cm, diameter 0.5~0.8cm, interior containing tens of thousands of
To ten tens of thousands of seeds, tiny such as dust-like observes that kernel texture is very simple, only there are one simple embryos under the microscope
And cell monolayer sticks up shape seed coat structure, no endosperm, seed length × wide 126 × 84 μm of average out to.Due to the seed of dendrobium candidum
It is very subtle, it is difficult to seed is separated, with conventional method be difficult to accurate counting capsule contained by grain weight, and seed is in natural shape
It is not easy to maintain under state, it is easy to inactivate.
Invention content
Easy, highly practical Seeds of Dendrobium Candidum seedling is used the technical problem to be solved in the present invention is to provide a kind of
Counting method provides accurately guidance and reference with the seed amount in accurate counting dendrobium candidum capsule for growing dendrobium seedlings.
In order to solve the above technical problems, the present invention uses following technical problem：The seedling counting method of Seeds of Dendrobium Candidum, will
For the seed germination and growth of dendrobium candidum at seedling, the quantity by calculating seedling obtains the quantity of seed.
The seedling counting method of above-mentioned Seeds of Dendrobium Candidum, the seed in dendrobium candidum capsule is broadcast to be made in media surface
Its germination and growth is at seedling.
Culture medium group become MS+1.5mg/L 6-BA+0.5mg/LNAA+200g/L potato leachate+20g/L sucrose+
5g/L agar, pH5.8.
Seedling is averaged plant height more than or equal to 5.0mm.
The seedling counting method of above-mentioned Seeds of Dendrobium Candidum, includes the following steps：
<1>It is prepared by culture medium
Culture medium is prepared by formula, the flat bottom glass bottle packing of diameter 6cm, high 10cm is used in combination then to be set per bottled 25ml
121℃、1.1kg/cm2Pressure under sterilize 20min, for use；
<2>Seed treatment
Dendrobium candidum capsule uses liquid detergent aqueous solution soaking 5min, tap water to rinse 15-30min successively, then ultra-clean
1g/LHgCl is put on workbench2Sterilize 15min in solution, and after aseptic water washing 5 times, aseptic filter paper blots fruit surface water
Point, for use；
<3>Seed is sowed
Seed in capsule is accessed into media surface；
<4>Seed culture
The culture medium connected is put in 25 DEG C of culturing room, in fill-in light according to 10-12 hours/day, intensity of illumination 1500lux items
It is cultivated under part；
<5>Seed counting
Wait for that seed grows up to seedling, average plant height reaches 5.0mm, takes out whole seedlings and counts, obtains the quantity of seed in capsule.
For dendrobium candidum seed it is very subtle be difficult to separation and count and problem not easy to maintain, according to seed in capsule
The long period is can save, Seed storage is all made of the mode that capsule preserves in production, and inventor establishes a kind of dendrobium candidum kind
The seedling counting method of son, by the seed germination and growth of dendrobium candidum at seedling, the quantity by calculating seedling obtains the number of seed
Amount.Since the genealogy of law broadcasts seed that is subtle, being difficult in the dendrobium candidum capsule that using routine, direct discernable by eye counts
Media surface, the nutrition abundant using culture medium is prepared, sprouts for Seeds of Dendrobium Candidum and growth supplies nutrients and water
Point, it is ensured that the seed that its capsule preserves can survive that (mature seed 99% can sprout life on culture medium substantially on culture medium
It is long), and allow seed germination and growth that can understand a certain size the seedling (average plant height reaches 5.0mm) differentiated and counted at naked eyes,
Make it intuitive, easy counting, then can be obtained the quantity of seed by counting seedling quantity.The present invention uses easy, practical
Property it is strong, need not be by other more accurate microsurgical instrument equipment, so that it may with the seed number in accurate counting dendrobium candidum capsule
Amount, to conveniently obtain the quantity of production seedling, this provides accurately guidance and reference for extensive nursery and production.
Specific implementation mode
Embodiment
<1>It is prepared by culture medium
Culture medium group become MS+1.5mg/L 6-BA+0.5mg/LNAA+200g/L potato leachate+20g/L sucrose+
5g/L agar, pH5.8；
Culture medium is prepared by formula, the flat bottom glass bottle packing of diameter 6cm, high 10cm is used in combination then to be set per bottled 25ml
121℃、1.1kg/cm2Pressure under sterilize 20min, for use；
<2>Seed treatment
Late September to late November, harvesting health is full, free from insect pests, pericarp slightly turns yellow, do not crack ripe dendrobium candidum
Capsule uses liquid detergent aqueous solution soaking 5min, tap water to rinse 15-30min successively, removes extra carpopodium, base of fruit etc., then
1g/LHgCl is put on superclean bench2Sterilize 15min in solution, and after aseptic water washing 5 times, aseptic filter paper blots fruit table
Face moisture, for use；
<3>Seed is sowed
In superclean bench, an osculum is cut in one end of capsule, lightly shakes capsule, the seed in capsule is very thin
Ground equably accesses media surface；10 capsules, one number of each fruit, according to fruit size, per carpostrote tens are sowed altogether
Bottle is differed to more than hundred bottles；
<4>Seed culture
The culture medium connected is put in 25 DEG C of culturing room, in fill-in light according to 10-12 hours/day, intensity of illumination 1500lux items
It is cultivated under part；
<5>Seed counting
In carrying out dendrobium candidum test tube seedling incubation, at protocorm, protocorm continues to cultivate seed elder generation germination and growth
Just grow up to the complete seedling of root, stem, leaf, after the seed culture 100 days of powdery, seedling be averaged plant height up to 5.0mm (for proembryo
35.9 times), it can visually be easy to differentiate, take out whole seedlings of each fruit, as soon as with 20 plants for group counting, be easy to obtain each
Grain weight in a capsule.
As a result：Seed amount in 10 capsules is respectively 71500,43500,44500,26600,35196
It is a, 53762,133100,95800,85300,75600.
Claims (1)
1. a kind of seedling counting method of Seeds of Dendrobium Candidum, it is characterised in that broadcasting the seed in dendrobium candidum capsule in culture medium
Surface makes its germination and growth at seedling, and the quantity by calculating seedling obtains the quantity of seed；The culture medium group becomes MS+
1.5 mg/L 6-BA+0.5mg/LNAA+200g/L potato leachates+20g/L sucrose+5g/L agar, pH5.8；The method
Specifically include following steps：
<1>It is prepared by culture medium
Culture medium is prepared by formula, the flat bottom glass bottle packing of diameter 6cm, high 10cm is used in combination then to set 121 per bottled 25ml
℃、1.1kg/㎝2Pressure under sterilize 20min, for use；
<2>Seed treatment
Dendrobium candidum capsule uses liquid detergent aqueous solution soaking 5min, tap water to rinse 15-30min successively, then in ultra-clean work
1g/LHgCl is put on platform2Sterilize 15min in solution, and after aseptic water washing 5 times, aseptic filter paper blots fruit surface moisture, waits for
With；
<3>Seed is sowed
Seed in capsule is accessed into media surface；
<4>Seed culture
The culture medium connected is put in 25 DEG C of culturing room, under the conditions of fill-in light is according to 10-12 hours/day, intensity of illumination 1500lux
Culture；
<5>Seed counting
Wait for that seed grows up to seedling, average plant height is taken out whole seedlings and counted, obtain the quantity of seed in capsule up to 5.0 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510781009.5A CN105325297B (en) | 2015-11-13 | 2015-11-13 | The seedling counting method of Seeds of Dendrobium Candidum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510781009.5A CN105325297B (en) | 2015-11-13 | 2015-11-13 | The seedling counting method of Seeds of Dendrobium Candidum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105325297A CN105325297A (en) | 2016-02-17 |
| CN105325297B true CN105325297B (en) | 2018-07-17 |
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|---|---|---|---|
| CN201510781009.5A Expired - Fee Related CN105325297B (en) | 2015-11-13 | 2015-11-13 | The seedling counting method of Seeds of Dendrobium Candidum |
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- 2015-11-13 CN CN201510781009.5A patent/CN105325297B/en not_active Expired - Fee Related
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| CN105325297A (en) | 2016-02-17 |
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Granted publication date: 20180717 Termination date: 20191113 |