CN105293693B - A kind of anaerobic ammonia oxidizing bacteria cultural method based on zwitterion resin - Google Patents
A kind of anaerobic ammonia oxidizing bacteria cultural method based on zwitterion resin Download PDFInfo
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- CN105293693B CN105293693B CN201510832979.3A CN201510832979A CN105293693B CN 105293693 B CN105293693 B CN 105293693B CN 201510832979 A CN201510832979 A CN 201510832979A CN 105293693 B CN105293693 B CN 105293693B
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- 239000011347 resin Substances 0.000 title claims abstract description 18
- 229920005989 resin Polymers 0.000 title claims abstract description 18
- 241001453382 Nitrosomonadales Species 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000010802 sludge Substances 0.000 claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 19
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 230000000694 effects Effects 0.000 claims abstract description 14
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 238000001179 sorption measurement Methods 0.000 claims abstract 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 30
- 239000000203 mixture Substances 0.000 claims description 9
- 239000007799 cork Substances 0.000 claims description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 abstract description 2
- 229910021529 ammonia Inorganic materials 0.000 description 7
- 230000003647 oxidation Effects 0.000 description 7
- 238000007254 oxidation reaction Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- PDNNQADNLPRFPG-UHFFFAOYSA-N N.[O] Chemical compound N.[O] PDNNQADNLPRFPG-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 241000238370 Sepia Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
Needle of the present invention discloses a kind of sludge fast culture process of the anaerobic ammoxidation activity based on zwitterion resin adsorption nutrient matrix.Under anaerobic condition, will have or may mix, be positioned in reactor with zwitterion resin, certain density culture solution is added, cultivated and produced under the suitable condition of culture of anaerobic ammonia oxidizing bacteria with the sludge of anaerobic ammoxidation activity.Temporally adjustment water inlet culture solution concentration increases sludge quantity every a period of time.Zwitterion resin adsorption ammonia nitrogen and nitrite nitrogen, are tightly combined with anaerobic ammonia oxidizing bacteria, ensure there are enough nutrients in anaerobic ammonia oxidizing bacteria surrounding environment.Advantages of the present invention：1）：Culture easily obtains a large amount of anaerobic ammonia oxidizing bacterias；2）：The training method utilized is easy.The Anammox flco of efficient denitrification was obtained within 2 months time.
Description
Technical field
The present invention relates to a kind of anaerobic ammonia oxidizing bacteria fast enriching cultural methods, i.e., using zwitterion resin as carrier, inhale
Follower ring border nutriment provides high concentration nutritional element, promotes the method for anaerobic ammonia oxidizing bacteria growth..
Background technology
Anaerobic ammonia oxidation process has efficient, economic, section in processing high ammonia-nitrogen wastewater, especially in terms of low ratio of carbon to ammonium waste water
Can etc. evident characteristics, and have a good application prospect and commercial value.With going deep into for Anammox research, anaerobism ammonia oxygen
Change group technology also to grow up, is widely used in high ammonia-nitrogen wastewater.But in actual application, come by inoculum
Source, matrix start the influence of the factors such as time length, the startup ratio of anaerobic ammonia oxidation reactor from inhibition, exogenous poisonous substance and technique
It is more difficult.Mainly anaerobic ammonia oxidizing bacteria is slow-growing, constrains the fast development of technique.
Start Anammox at this stage and mostly use anaerobic sludge or anoxic sludge greatly, successfully to turn out anaerobism ammonia oxygen
It is mark to change bacterium or the granule sludge with Anammox effect.More start anaerobism ammonia using biomembrane as carrier both at home and abroad
Oxidation reactor, the anaerobic ammonia oxidizing bacteria cultivated are mostly graininess, and reactor is larger, cumbersome, and culture effect has
Have bad.For floc sludge, using the reactor of very little, high-volume culture is carried out, accomplishes that condition of culture is controllable in real time.
Invention content
The purpose of the invention is to which the effect for transmitting ion can be adsorbed using zwitterion resin, to ring around bacterium
Border provides high concentration nutritional substance, and high-volume cultivates anaerobic ammonia oxidizing bacteria, shortens anaerobic ammonia oxidation reactor and starts the time, provides
A kind of new-type anaerobic ammonia oxidation reactor startup method.The cultural method is easy to operate, can carry out Batch Culture, obtained
Anammox flco the operation is stable, nitric efficiency is high, and can shorten anaerobic ammonia oxidation reactor and start the time.
Method and step is as follows：1）It takes and pre- first passes through the floc sludge mixture of anaerobic ammoxidation activity culture for a period of time
100ml or so, being added to 500ml can use in the closed vial of cork, zwitterion resin about 100ml be added, grain size exists
1mm-2mm is advisable.Plug is stoppered, acutely rocks and ensures that sludge and zwitterion resin are uniformly mixed.150-200ml battalion is added
Nutrient solution, bottle are put upside down, and water-bath culture is based on, and water temperature control is advisable between 30-34 DEG C.Ammonia nitrogen, Asia are mixed in bottle per surveying for 24 hours
Nitrate and pH value.Per 48h, water inlet is primary, after water inlet, keeps NH in bottle4 +- N concentration is in 100-120mg/L, NO2 -- N is dense
Degree is in 130-150mg/L, unsuitable excessively high, NaHCO3A concentration of 1500-1800mg/L.Ammonia nitrogen, nitrite nitrogen increase with time
Concentration.Water inlet is ammonia nitrogen in high density, nitrite nitrogen and inorganic carbon source solution.
2）Using NH4 +With NO2 -The ratio between concentration is 1：1.3 water inlet, control inlet flow-patterm were cultivated between 7.5-8.0 values
Journey is scaling up a NH at any time4 +- N and NO2 -- N concentration, each NH4 +- N concentration increases by 20 ~ 40mg/L, NO2 -- N concentration
Increase by 26 ~ 52mg/L.The time for increasing concentration utilizes the speed control of ammonia nitrogen and nitrite nitrogen according to sludge.
Benefit of the invention is that using the floc sludge mixture of anaerobic ammoxidation activity culture for a period of time is first passed through in advance
It is cultivated, added zwitterion resin has the function of adsorbing ammonia nitrogen and nitrite nitrogen, uninterrupted for bacterial growth structure
, the nutriment of high concentration.Succeed Batch Culture anaerobic ammonia oxidizing bacteria within 2 months, at this time NH4 +And NO2 -Removal rate
95% or more.Meanwhile according to sludge quantity and sludge to NH4 +And NO2 -Utilization power to intake in due course adjustment, effectively facilitate dirt
The culture of mud.
Specific implementation mode
The present invention is cultivated using the floc sludge mixture of anaerobic ammoxidation activity culture for a period of time is first passed through in advance,
The Clear glass bottles and jars for the tool cork that reactor is dischargeable capacity 500ml.By monitoring and adjusting culture solution water-based concentration, to detest
The growth of anaerobic ammonium oxidation bacterium provides best conditions, to achieve the purpose that anaerobic ammonia oxidizing bacteria fast-growth.Incubation operation letter
It is single, Batch Culture can be carried out, biological total amount is increased.
The present invention using the above method to by anaerobic ammoxidation activity culture floc sludge mixture for a period of time into
The operating method of row culture is as follows：1, first passing through the pre- of experiment first passes through the wadding of anaerobic ammoxidation activity culture for a period of time in advance
Shape mud mixture is the sludge that laboratory is independently cultivated, and sludge is mixing sludge, derives from multiple sewage treatment plants or nature
Waters.Take it is pre- first pass through the floc sludge mixture 100ml of anaerobic ammoxidation activity culture for a period of time, zwitterion tree is added
Fat is total to about 100g.Zwitterion resin washes away surface impurity with cycle tap water in advance, until after supernatant clarification, precipitates and abandons
Supernatant is removed, resin is mixed and is added in vials of the 500ml with cork, is acutely rocked, ensures the complete of sludge and resin
Mixing.Bottle is based on water-bath culture, and water temperature controls between 30-34 DEG C.Per surveying for 24 hours, mixing ammonia nitrogen, nitrite nitrogen in bottle are dense
Degree and pH value.Per 48h, water inlet is primary, intakes as ammonia nitrogen in high density, nitrite nitrogen and inorganic carbon source solution.Ammonia increase with time
The concentration of nitrogen, nitrite nitrogen.
2, using NH4 +With NO2 -The ratio between concentration is 1：1.3 water inlet, control inlet flow-patterm is between 7.8-8.0 values, with advance
Floc sludge mixture by anaerobic ammoxidation activity culture is culture sludge.Initial stage of culture water inlet NH4 +- N is a concentration of
100mg/L, NO2 -A concentration of 132mg/L of-N, are scaling up NH at any time4 +- N concentration and NO2 -- N concentration, each NH4 +- N is dense
Degree increases by 20 ~ 40mg/L, NO2 -- N concentration increases by 26 ~ 52mg/L, until NH4 +- N concentration increases to 200mg/L, NO2 -- N concentration increases
It is added to 264mg/L.
Embodiment
This experiment is carried out using in vials of 20 500ml with cork.Sludge source is in multiple sewage disposals
Factory or natural water area.Sludge color is khaki, without apparent smell.It takes and pre- first passes through anaerobic ammoxidation activity culture for a period of time
Floc sludge mixture 100ml or so, being added to 500ml can use in the closed vial of cork, and zwitterion resin is added
About 100ml stoppers plug, acutely rocks and ensures that sludge and zwitterion resin are uniformly mixed.150-200ml nutrition is added
Liquid, bottle are put upside down, and water-bath culture is based on, and water temperature control is advisable between 30-34 DEG C.Ammonia nitrogen, nitrous are mixed in bottle per surveying for 24 hours
State nitrogen concentration and pH value.Per 48h, water inlet is primary, after water inlet, keeps NH in bottle4 +- N concentration is in 100-120mg/L, NO2 -- N concentration
In 130-150mg/L, unsuitable excessively high, NaHCO3A concentration of 1500-1800mg/L.Increase with time ammonia nitrogen, nitrite nitrogen it is dense
Degree.Water inlet is ammonia nitrogen in high density, nitrite nitrogen and inorganic carbon source solution.
Using NH4 +With NO2 -The ratio between concentration is 1：1.3 water inlet, control inlet flow-patterm is between 7.8-8.0 values, and water inlet is by certainly
Water configures, therefore does not add trace element.Incubation is scaling up a NH at any time4 +- N and NO2 -- N concentration, every time
NH4 +- N concentration increases by 20 ~ 40mg/L, NO2 -- N concentration increases by 26 ~ 52mg/L.The time for increasing concentration utilizes ammonia nitrogen according to sludge
With the speed control of nitrite nitrogen.
In first 20 days, NH4 +- N and NO2 -The ratio of the removal amount of-N fluctuates between 1.2-1.5, to ammonia nitrogen and nitrous state
The utilization of nitrogen has larger difference, and the gas that sludge generates is less；20th day to the 40th day, NH4 +- N and NO2 -The removal amount of-N
Ratio is between 1.3-1.4, and pH value of solution keeps stablizing, while generating more gas, and sludge has the phenomenon that floating；40th day to
60 days, NH4 +- N and NO2 -The removal amount ratio of-N between 1.3-1.4, stablize by pH value of solution, and sludge generates bulk gas, sludge
Obviously there is hydro-planing.Sludge integral color is gradually transitioned into sepia by khaki, finally shows bronzing, some bottles are in palm fibre
The surface of brown mud, it is apparent that red granules shape sludge shows anaerobic ammonia oxidizing bacteria culture success.
Claims (1)
1. the anaerobic ammonia oxidizing bacteria cultural method based on zwitterion resin adsorption, which is characterized in that the step of the method such as
Under：
1）Take it is pre- first pass through the floc sludge mixture 100ml of anaerobic ammoxidation activity culture for a period of time, being added to 500ml can
With in the closed vial of cork, zwitterion resin 100ml, grain size 1-2mm is added, plug is stoppered, guarantor is acutely rocked
It demonstrate,proves sludge and zwitterion resin is uniformly mixed, 150-200ml nutrient solutions are added, bottle is put upside down, and water-bath culture, water temperature control are based on
System is between 30-34 DEG C, and per mixing ammonia nitrogen, nitrite nitrogen concentration and pH value in bottle is surveyed for 24 hours, per 48h, water inlet is primary, water inlet
Afterwards, NH in bottle is kept4 +- N concentration is in 100-120mg/L, NO2 -- N concentration is in 130-150mg/L, NaHCO3A concentration of 1500-
1800mg/L, the concentration of ammonia nitrogen and nitrite nitrogen increase with time, intake for ammonia nitrogen in high density, nitrite nitrogen and inorganic carbon source it is molten
Liquid；
2）Using NH4 +With NO2 -The ratio between concentration is 1：1.3 water inlet, control inlet flow-patterm between 7.5-8.0 values, incubation with
Time is scaling up a NH4 +- N and NO2 -- N concentration, each NH4 +- N concentration increases by 20 ~ 40mg/L, NO2 -- N concentration increases
26 ~ 52mg/L, the time for increasing concentration utilize the speed control of ammonia nitrogen and nitrite nitrogen according to sludge.
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| CN106746178B (en) * | 2016-11-23 | 2020-03-20 | 南京华淳环保股份有限公司 | Advanced sewage denitrification process based on chemical separation-physical transfer of nitrate and nitrogen |
| CN109179911A (en) * | 2018-10-31 | 2019-01-11 | 北京安国水道自控工程技术有限公司 | A kind of deep denitrogenation device and method of city domestic sewage |
| CN109095733A (en) * | 2018-10-31 | 2018-12-28 | 北京安国水道自控工程技术有限公司 | A kind of deep denitrogenation device and method of low ratio of carbon to ammonium city domestic sewage |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101343116A (en) * | 2008-07-18 | 2009-01-14 | 北京工业大学 | Quick start method for anaerobic ammonia oxidation bioreactor for urban sewage |
| CN102976485A (en) * | 2012-12-10 | 2013-03-20 | 东北电力大学 | Method and device for fast cultivation of anaerobic granular sludge for treating swine wastewater |
| CN103508555A (en) * | 2013-10-08 | 2014-01-15 | 中国石油天然气集团公司 | Reactor for realizing suspension of biofilm carriers under low aeration quantity |
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| JP2006122771A (en) * | 2004-10-27 | 2006-05-18 | Chuden Kankyo Technos Co Ltd | Fluid treatment method and fluid treatment system |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101343116A (en) * | 2008-07-18 | 2009-01-14 | 北京工业大学 | Quick start method for anaerobic ammonia oxidation bioreactor for urban sewage |
| CN102976485A (en) * | 2012-12-10 | 2013-03-20 | 东北电力大学 | Method and device for fast cultivation of anaerobic granular sludge for treating swine wastewater |
| CN103508555A (en) * | 2013-10-08 | 2014-01-15 | 中国石油天然气集团公司 | Reactor for realizing suspension of biofilm carriers under low aeration quantity |
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