CN105288168A - Pharmaceutical composition for treating keratitis caused by liver and gall with exuberant fire and preparation method thereof - Google Patents

Abstract

The invention discloses a medicine for treating keratitis caused by the liver and gall with exuberant fire and a preparation method thereof. The medicine is prepared from the following medicinal materials: selfheal, himalayan prinsepia fruits, ox-gall, lysimachia christinae hance, Tibetan capillary, Chinese knotweed herb, feather cockscomb seeds, chingma abutilon seeds, equisetum hiemale, radix bupleuri, chrysanthemum indicum, radix angelicae, cortex mori radicis, radix trichosanthis, oyster, hibiscus llutabilis, the bark of ash, whorledleaf solomonseal rhizome, hippuris vulgaris and moutan bark. The medicine disclosed by the invention is mainly used for treating keratitis caused by the liver and gall with exuberant fire, and effectively controlling disease development; repeated infection is not easily generated after recovery; the medicine effect directly reaches the lesion; the preparation technology is simple and convenient; and the medicine has the advantages of being significant in curative effect, free of a toxic or side effect, low in cost and convenient to take.

Description

A kind of pharmaceutical composition and preparation method for the treatment of liver and gall heated type keratitis

Technical field

The present invention relates to pharmaceutical technology, particularly relate to a kind of pharmaceutical composition and preparation method for the treatment of liver and gall heated type keratitis.

Background technology

Keratitis is common a kind of ophthalmic diseases, is the general name that cornea tissue is inflamed, with corneal clouding, cornea superfluous life, corneal ulcer, perforation of cornea and leave aglia for feature.Keratitis is divided into ulcerative keratitis (corneal ulcer), and non-ulcerative keratitis (deep keratitis) two class, by endogenous cause of ill, exopathogenic factor Different factor causes.

Reason:

One, exopathogenic factor: the damage of corneal epithelial cell, to come off, only when these two conditions all possess, just easily there is infective corneal ulcer in concurrent infection simultaneously;

Two, endogenous cause of ill: refer to the endogenous illness from whole body.Because cornea does not have blood vessel, so acute infectious disease is not easily invaded and cornea.Cornea tissue but participates in the immunoreation of whole body, although its immunoreactive degree is low compared with its hetero-organization, but just because of it does not have blood vessel, metabolism is comparatively slow, this immunoreation is just made to change lasting prolonged, cornea is in a kind of sensitiveness in a long time, so that easily allergy illness occurs, as phlyctenular keratitis etc.;

Three, adjacent tissue is spread: due to the affinity in fetology and seriality anatomically, and the illness spreading to corneal epithelium is many from conjunctiva, as serious conjunctivitis many merging keratitis superficialis.

Symptom: ciliary congestion, corneal infiltration muddiness and corneal ulcer are formed, and are the basic signs of keratitis.Because cornea has abundant nervi trigeminus tip, the most obvious symptom of keratitis is ophthalmalgia, and normal sustainable existence is until inflammatory resolution.Twinkle motion time, because of upper eyelid friction cornea, ophthalmalgia is more obvious.Due to the struvite contracture of iris vessels, photophobia can be caused, shed tears, blepharospasm etc., the severe patient corneal opacity, ophthalmic secretions increase.Antibacterial or fungus-caused suppurative keratitis, often with the more purulent secretion of various trait; Viral keratitis then secretions is few.Normal with visual deterioration in various degree, if pathological changes is positioned at lesser ring of Merkel, then visual deterioration is more obvious.

Typing:

The traditional Chinese medical science treats in line with integral syndrome differentiation, according to keratitis Clinical presentation and feature, by its typing is:

1, liver and gall heated type: disease sees that suffering from eye mixes red, the heavier companioned with headache of pain or eye socket distending pain, bitter taste in the mouth and dry throat, and cornea is shown in dendroid or map shape, fluorescein (+), or corneal edema is muddy with ulcer, iritis, yellow fur, wiry and frequent pulse.

2, liver meridian wind-heat type: have photophobia, sheds tears, pain, and corneal epithelium point-like infiltrates, fluorescein (+), red tongue, yellow and thin fur, floating and rapid pulse.

3, liver-blood deficiency type: suffer from that furious red very pain, photophobia are comparatively not light, dry and astringent discomfort, blurred vision, delay with the passing of time, red tongue with a little fluid, thready and rapid pulse.

Check:

1, according to clinical manifestation, pain, photophobia, shed tears, the irritation such as blepharospasm, and ciliary congestion, corneal infiltration are muddy or ulcer etc., namely diagnosable.Etiological diagnosis and early diagnosis should be emphasized.Detailed medical history-taking, as ocular injury, premorbid is with or without cold, fever history, and local or whole body use glucocorticoid or immunosuppressant, with or without relevant general disease etc., can point out the cause of disease of keratitis.Should be conventional before pathological changes not yet uses antibiotic in early days, carry out keratopathy district scraping blade microscopy, microorganism culturing and drug sensitivity test.

2, the visible cornea gloss disappearance of ophthalmologic examination, transparency attenuating, ulcer, ciliary congestion.Divide antibacterial, virus, fungal keratitis etc. by the cause of disease, be divided into central part, periphery keratitis by the region of anatomy, be divided into point-like, wire, plate-like, money shape, dendroid, map shape keratitis by clinical manifestation.Corneal epithelium, if any defect, more easily finds with fluorescent staining.

Harm: keratitis is a kind of serious blinding disease, can cause visual deterioration in various degree, and the course of disease of this oculopathy is long, recovers slow, if controlled not in time, can further develop becomes corneal ulcer and even bore a hole, and finally may need corneal transplantation.

At present, the treatment of doctor trained in Western medicine corneal inflammation, the eye drop that general antimicrobial drug adds cortex amcinonide share to be controlled, as oral western medicine antibiotic, oral hormone, externally applied anti-inflammation eye drop etc., curative effect is unstable, easily recurrence and side effect is large, long period or the heavy dose of external hormone preparation of short-term, also addiction can cause drug dependence.

Chinese traditional treatment keratitis possesses unique advantage and feature, it according to Chinese medicine differentiation of symptoms and signs to identify etiology, examine the principle controlled because of opinion, liver heat removing dispelling wind, improving acuity of vision and removing nebula, heat-clearing and toxic substances removing, hit pathogenesis, and improve on the whole and regulate body viscera function, therefore stable curative effect and not easily recurring.

Summary of the invention

Technical problem to be solved by this invention is, provides a kind of medicine for the treatment of liver and gall heated type keratitis, symptom management development effectively, not easily repeated infection more, drug effect is gone directly focus, and processing technology is easy, has evident in efficacy, have no side effect, with low cost, the advantage of taking convenience.

For solving the problems of the technologies described above, the invention provides a kind of medicine for the treatment of liver and gall heated type keratitis, wherein, comprising following raw medicinal material: Spica Prunellae, Fructus prinsepiae utilis, Fel Bovis seu Bubali ,herba Lysimachiae, Tibetan Artemisia anethoicles Mattf, Herb Polygoni Chinensis ,semen Celosiae, Semen Abutili, Herba Equiseti Hiemalis, Radix Bupleuri, Herba Dendranthematis indici, the Radix Angelicae Dahuricae, Cortex Mori, Radix Trichosanthis, Concha Ostreae, Flos Hibisci Mutabilis, Cortex Fraxini, Rhizoma Polygonati Verticillati, Herba Hippuridis Vulgaris and Cortex Moutan.

Wherein, in described medicine, the parts by weight of each raw medicinal material can be preferably: Spica Prunellae 13-32 part, Fructus prinsepiae utilis 9-19 part, Fel Bovis seu Bubali 10-28 part, Herba Lysimachiae 6-17 part, Tibetan Artemisia anethoicles Mattf 12-27 part, Herb Polygoni Chinensis 11-23 part, Semen Celosiae 8-22 part, Semen Abutili 7-15 part, Herba Equiseti Hiemalis 13-25 part, Radix Bupleuri 15-38 part, Herba Dendranthematis indici 14-32 part, Radix Angelicae Dahuricae 13-34 part, Cortex Mori 11-25 part, Radix Trichosanthis 6-16 part, Concha Ostreae 11-26 part, Flos Hibisci Mutabilis 13-30 part, Cortex Fraxini 7-18 part, Rhizoma Polygonati Verticillati 14-32 part, Herba Hippuridis Vulgaris 9-16 part and Cortex Moutan 13-31 part.

Wherein, in described medicine, the parts by weight of each raw medicinal material can also be preferably: Spica Prunellae 25 parts, Fructus prinsepiae utilis 9 parts, Fel Bovis seu Bubali 12 parts, Herba Lysimachiae 7 parts, Tibetan Artemisia anethoicles Mattf 16 parts, Herb Polygoni Chinensis 21 parts, Semen Celosiae 11 parts, Semen Abutili 8 parts, Herba Equiseti Hiemalis 23 parts, Radix Bupleuri 32 parts, 29 parts, Herba Dendranthematis indici, the Radix Angelicae Dahuricae 32 parts, Cortex Mori 18 parts, Radix Trichosanthis 7 parts, Concha Ostreae 20 parts, Flos Hibisci Mutabilis 25 parts, Cortex Fraxini 11 parts, Rhizoma Polygonati Verticillati 30 parts, Herba Hippuridis Vulgaris 13 parts and Cortex Moutan 24 parts.

Wherein, in described medicine, the parts by weight of each raw medicinal material can be preferably again: Spica Prunellae 28 parts, Fructus prinsepiae utilis 10 parts, Fel Bovis seu Bubali 13 parts, Herba Lysimachiae 9 parts, Tibetan Artemisia anethoicles Mattf 24 parts, Herb Polygoni Chinensis 13 parts, Semen Celosiae 19 parts, Semen Abutili 8 parts, Herba Equiseti Hiemalis 22 parts, Radix Bupleuri 35 parts, 30 parts, Herba Dendranthematis indici, the Radix Angelicae Dahuricae 27 parts, Cortex Mori 15 parts, Radix Trichosanthis 11 parts, Concha Ostreae 20 parts, Flos Hibisci Mutabilis 28 parts, Cortex Fraxini 9 parts, Rhizoma Polygonati Verticillati 30 parts, Herba Hippuridis Vulgaris 12 parts and Cortex Moutan 29 parts.

Wherein, in described medicine, the parts by weight of each raw medicinal material can be preferably again: Spica Prunellae 31 parts, Fructus prinsepiae utilis 17 parts, Fel Bovis seu Bubali 20 parts, Herba Lysimachiae 9 parts, Tibetan Artemisia anethoicles Mattf 25 parts, Herb Polygoni Chinensis 15 parts, Semen Celosiae 10 parts, Semen Abutili 11 parts, Herba Equiseti Hiemalis 19 parts, Radix Bupleuri 33 parts, 28 parts, Herba Dendranthematis indici, the Radix Angelicae Dahuricae 32 parts, Cortex Mori 17 parts, Radix Trichosanthis 7 parts, Concha Ostreae 19 parts, Flos Hibisci Mutabilis 16 parts, Cortex Fraxini 12 parts, Rhizoma Polygonati Verticillati 27 parts, Herba Hippuridis Vulgaris 12 parts and Cortex Moutan 27 parts.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method for the treatment of the medicine of liver and gall heated type keratitis, and when the dosage form of described medicine is eye drop, its preparation method comprises the following steps:

The first step, gets described raw medicinal material and adds soak by water 2 times, and first time adds 4-6 times amount soak by water 1-2 hour, filter, second time adds 3-5 times amount soak by water 1-2 hour, filters, 2 filtrates merged, when concentrating under reduced pressure obtains 80 DEG C, relative density is the extractum 1 of 1.04-1.10, and medicinal residues are for subsequent use;

Second step, medicinal residues are added 60-90% soak with ethanol 0.5-1.5 hour, extract twice, each 1-2 hour, merge extractive liquid, 80-160 mesh filter screen filters, 6000-10000 rev/min centrifugal, getting supernatant, is the ultrafiltration post ultrafiltration of 5000-10000 through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 60 DEG C 1.25 extractum 2;

3rd step, mixes extractum 1 and extractum 2, adds sodium chloride and the 60-80 distilled water doubly of 0.06% of mixing extractum gross mass, and to add concentration be that 4% sodium hydroxide solution regulates PH to be 6.0-7.0, then add 0.2% active carbon and boil 10 minutes, filter, subpackage, sterilizing, obtains eye drop.

The beneficial effect that the technical scheme that the embodiment of the present invention provides is brought is: utilize the theory of Chinese medical science that China is traditional, provides a kind of medicine curing mainly liver and gall heated type keratitis, can play purging liver-fire, function of gallbladder promoting detoxifies, and improving acuity of vision and removing nebula is main, YIN nourishing and the production of body fluid promoting, clots absorbing eliminating stagnation, blood-activating and qi-promoting are auxiliary therapeutical effect, symptom management development effectively, not easily repeated infection more, drug effect is gone directly focus, and processing technology is easy, has evident in efficacy, have no side effect, with low cost, the advantage of taking convenience.

Detailed description of the invention

The invention provides a kind of medicine for the treatment of liver and gall heated type keratitis, wherein, comprise following raw medicinal material: Spica Prunellae, Fructus prinsepiae utilis, Fel Bovis seu Bubali ,herba Lysimachiae, Tibetan Artemisia anethoicles Mattf, Herb Polygoni Chinensis ,semen Celosiae, Semen Abutili, Herba Equiseti Hiemalis, Radix Bupleuri, Herba Dendranthematis indici, the Radix Angelicae Dahuricae, Cortex Mori, Radix Trichosanthis, Concha Ostreae, Flos Hibisci Mutabilis, Cortex Fraxini, Rhizoma Polygonati Verticillati, Herba Hippuridis Vulgaris and Cortex Moutan.

Wherein, the pharmacology of above-mentioned raw materials medical material is as follows:

Spica Prunellae: acrid in the mouth, hardship, cold in nature.Return liver, gallbladder meridian.Relieve inflammation or internal heat, improving eyesight, eliminating stagnation, detumescence.For conjunctival congestion and swelling pain, order pearl nyctalgia, has a headache dizzy, scrofula, goiter, and acute mastitis swells and ache; Thyromegaly, tuberculous lymphadenitis, cyclomastopathy, hypertension.

Fructus prinsepiae utilis: another name buy oil fruit, shoot fruit, gun barrel fruit, milk hammer, Canis familiaris L. milk, be the fruit of rosaceous plant Folium Prinsepiae Utilis.Bitter in the mouth, acid; Cool in nature.Return Liver Channel.Invigorating the stomach and promoting digestion, improving acuity of vision and removing nebula, removing toxic substances.Main accumulation of food in the stomach and intes tine due to indigestion, the many tear of corneal nebula, sore carbuncle

Fel Bovis seu Bubali: the gallbladder of bovid cattle or Babalus bubalis L. or bile, from slaughtering an ox, field is collected, and obtains rear hang-up and dries in the shade or cut off from bile duct, by bile impouring container, sealing storage.Or heating makes it drying and also can.Bitter in the mouth, property Great Cold."

Enter liver, gallbladder, lung three warp.Liver heat removing and eyesight improving, promoting the secretion of bile and dredging bowels, removing toxic substances and promoting subsidence of swelling, control wind heat order disease, jaundice, constipation, quenches one's thirst, infantile convulsion, carbuncle hemorrhoid.

Herba Lysimachiae: another name Herba Lysimachiae, to seat grass, LUBIANHUANG, everywhere Huang, Hydrocotyle vulgaris L., Yi Chuanqian, CUNGU seven, be the dry herb of Ofthe Primulaceae Lysimachia christinae Hance.Summer, Qiu Erji gather, and removing impurity, dries.Sweet in the mouth, salty, cold nature.

Return liver, gallbladder, kidney, urinary bladder channel.Eliminating damp-heat, treating stranguria, detumescence.For pyretic stranguria, Sha Lin, urinates and puckeryly to have a pain, jaundice dark coloured urine, carbuncle furuncle, venom; Liver and bile stone, lithangiuria.

Hide Artemisia anethoicles Mattf: be the herb of gentianaceae plant Swertia mussotii Franch..Gather and store: summer, autumn gather, cleaning, chopping, dries.Bitter in the mouth; Cool in nature.Liver heat removing promoting the function of the gallbladder to alleviate jaundice; Inducing diuresis to remove edema.The main permanent type of acute Huang and non-icterohepatitis; Cholecystitis; Edema

Herb Polygoni Chinensis: another name wing land productivity, Linesless charcoal star, Linesless charcoal rattan, plain rice grass, plain rice rattan, letter meal rattan are Polygonaceae arsesmart Herb Polygoni Chinensis, with all herbal medicine.The four seasons can adopt, and clean and dry or using fresh herb.The micro-acid of taste, micro-puckery, cool in nature.Heat-clearing and toxic substances removing, dampness removing disappears stagnant, blood-cooling itch-relieving, improving acuity of vision and removing nebula.For dysentery, enteritis, dyspepsia, hepatitis, flu, tonsillitis, pharyngolaryngitis, diphtheria, pertussis, corneal nebula, colpitis mycotica, leucorrhea, mastitis, furuncle and phyma, children's's impetigo, eczema, venom.

Semen Celosiae: call wild Flos Celosiae Cristatae, Herba Desmodii Heterocarpi, Canis familiaris L. tail Herba Amaranthi tricoloris is the dry mature seed of amaranthaceous plant Herba Celosiae argenteae.Tap plant during fruit maturation in autumn or win fruit ear, drying, collecting seed, removing impurity.Bitter in the mouth, cold nature.Return Liver Channel.Liver heat removing, improving eyesight, nebula removing.For liver-heat conjunctival congestion, nebula of looking unfamiliar film, blurring of vision, dizziness due to liver-fire.

Semen Abutili: another name piemarker, wild cotton beggar, hemp are the dry mature seed of Malvaceae plant Herba Abutili.To gather autumn mature fruit, dry, lay seed, removing impurity.Bitter in the mouth, property is put down.Return large intestine, small intestinal, urinary bladder channel.Clearing away heat-damp and promoting diuresis, removing toxic substances, nebula removing.For dysentery, the puckery pain of gonorrhea, carbuncle, corneal nebula.

Herba Equiseti Hiemalis: another name file grass, nib grass, Herba equiseti ramosissimi, Herba Equiseti Hiemalis are the dry aerial parts of Equisetaceae plant Herba Equiseti Hiemalis.Summer, Qiu Erji tap, and removing impurity, dries or dry in the shade.Sweet in the mouth, hardship, property is put down.Return lung, Liver Channel.Loose wind heat, moves back corneal nebula.For wind heat conjunctival congestion, epiphora induced by wind, the raw nebula of order.

Radix Bupleuri: bitter in the mouth, cold nature.Return liver, gallbladder meridian.Reconciling superficies and interior, soothing the liver, yang invigorating.For cold, fever, alternate attack of chill and fever, distending pain in the chest and hypochondrium, menoxenia, sub-official's prolapsus, proctoptosis.

Herba Dendranthematis indici: bitter in the mouth, pungent, cold nature, returns liver, heart channel.There is effect of heat-clearing and toxic substances removing, cure mainly furuncle carbuncle, conjunctival congestion and swelling pain, have a headache dizzy.

The Radix Angelicae Dahuricae: acrid in the mouth, warm in nature.Return stomach, large intestine, lung meridian.Loose wind dehumidifying, understand things pain-stopping, detumescence and apocenosis.For headache due to common cold, supraorbital bone pain, nasal obstruction, nasal sinusitis, toothache, leucorrhea, skin infection swells and ache.

Cortex Mori: another name Radix Mori skin is the dry root bark of moraceae plants Mulberry.Fall last years of a century in autumn and excavate root before secondary spring germinates, scrape off yellowish-brown rough bark, longitudinally cut open, strip root bark, dry.Sweet in the mouth, cold in nature.Return lung meridian.Eliminating pathogen from the lung for relieving asthma, inducing diuresis to remove edema.For dyspnea and cough due to lung-heat, edema distension oliguria, appearance skin edema.

Radix Trichosanthis: be the dry root of cucurbitaceous plant Fructus Trichosanthis or trichosanthes rosthornii Harms.Autumn, season in winter two excavate, and clean, removing crust, and cutting or vertical profile become lobe, dry.Sweet in the mouth, micro-hardship, cold nature.Attach to the lung and stomach meridians.Clearing away heat and promoting production of body fluid, detumescence and apocenosis.For calentura excessive thirst, lung-heat type cough, interior-heat is quenched one's thirst, sore swollen toxin.

Concha Ostreae; Salty in the mouth, cold nature.Return liver, gallbladder, kidney channel, tranquillization with heavy prescription, YANG hyperactivity suppressing nourishing YIN, hard masses softening and resolving.For insomnia of palpitating with fear, vertigo and tinnitus, scrofula sucutaneous nodule, lump in the abdomen mass in the abdomen.Concha Ostreae (calcined) convergence is astringent or styptic treatment for spontaneous sweating.For spontaneous sweating, seminal emission metrorrhagia and leukorrhagia, stomachache acid regurgitation.

Flos Hibisci Mutabilis: another name Flos Hibisci Mutabilis (" clear different record "), ground Flos Hibisci Mutabilis (" Bencao Tujing "), refuses frostwork (" beneficial portion square object is note slightly "), seven-star flower (" conventional glossary of Herbs and Drugs among the people "), Herba Limnophilae Aromaticae, flower in the Frost's Descent (" Fujian Chinese herbal medicine ").For the flower of Malvaceae plant Hibisci Mutabilis .october plucks just open flower, dries.Acrid in the mouth, property is put down.Enter lung, liver.Heat clearing away, removing heat from blood, detumescence, removing toxic substances, controls carbuncle, furuncle, scalds, cough due to lung-heat, spits blood, metrorrhagia, leucorrhea.

Cortex Fraxini: other Ming Ash skin is dry branch skin or the dried bark of Oleaceae plants fraxinus rhynchophylla Hance, Chinese ash, sharp leaf Chinese ash or place post Chinese ash.Bitter in the mouth, puckery, cold in nature.Return liver, gallbladder, large intestine channel.Heat clearing and damp drying, astringent therapy, improving eyesight.For hematodiarrhoea, have loose bowels, leucorrhea with red and white discharge, conjunctival congestion and swelling pain, the raw nebula film of order.

Rhizoma Polygonati Verticillati: call smelly youngster ginseng, the wild Pseudobulbus Bletillae (Rhizoma Bletillae), Liliaceae HUANGJING ZANYU CAPSULE Gansu Rhizoma Polygonati, is used as medicine with root stock.Xia Qiu gathers, and cleans and dries, and place Hui Run, section is dried.Sweet in the mouth, property is put down.Nourishing the liver and kidney, YIN nourishing and the production of body fluid promoting.For neurasthenia, hypertension, headache, throat pain, quenches one's thirst, dry mouth and tougue.

Herba Hippuridis Vulgaris: the another name Qin pulls, and Hippuridaceae Herba Hippuridis Vulgaris, with all herbal medicine.Taste is micro-sweet, bitter, cool in nature.Antitussive, soothing liver-QI, cooling blood for hemostasis, YIN nourishing and the production of body fluid promoting, deep steaming.Cure mainly high hot excessive thirst, tuberculosis is coughed, the hot hectic fever due to YIN-deficiency of consumptive disease, pain in the hypochondrium, gastroenteritis etc.Stem, leaf external control traumatic hemorrhage.

Cortex Moutan: another name Cortex Moutan, unpeeled CORTEX MOUTAN, wooden Radix Paeoniae, bar Cortex Moutan, Luoyang flower are the dry root bark of ranunculaceae peony.Autumn excavates root, and removing radicula, strips root bark, dry.Bitter in the mouth, pungent, cold nature.GUIXIN, liver, kidney channel.Clearing away heat and cooling blood, blood circulation promoting and blood stasis dispelling.For maculae caused by violent heat pathogen, hematemesis and epistaxis, night fever abating at dawn, lossless hectic fever due to YIN-deficiency, amenorrhea dysmenorrhea, carbuncle sore tumefacting virus, falls and flutters the pain of injury.

Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.

Embodiment 1 embodiments provides a kind of medicine for the treatment of liver and gall heated type keratitis, is obtained by the raw medicinal material of following weight: Spica Prunellae 25g, Fructus prinsepiae utilis 9g, Fel Bovis seu Bubali 12g, Herba Lysimachiae 7g, Tibetan Artemisia anethoicles Mattf 16g, Herb Polygoni Chinensis 21g, Semen Celosiae 11g, Semen Abutili 8g, Herba Equiseti Hiemalis 23g, Radix Bupleuri 32g, Herba Dendranthematis indici 29g, Radix Angelicae Dahuricae 32g, Cortex Mori 18g, Radix Trichosanthis 7g, Concha Ostreae 20g, Flos Hibisci Mutabilis 25g, Cortex Fraxini 11g, Rhizoma Polygonati Verticillati 30g, Herba Hippuridis Vulgaris 13g and Cortex Moutan 24g.

The first step, gets described crude drug and adds soak by water 2 times, and first time adds 5 times amount soak by water 2 hours, filters, and second time adds 4 times amount soak by water 1 hour, filters, and 2 filtrate liquid is merged, and when concentrating under reduced pressure obtains 80 DEG C, relative density is the extractum 1 of 1.10, and medicinal residues are for subsequent use;

Medicinal residues are added 80% soak with ethanol 1 hour by second step, extract twice, each 1.5 hours, merge extractive liquid, 140 mesh filter screens filter, and 8000 revs/min are centrifugal, getting supernatant, is the ultrafiltration post ultrafiltration of 6000 through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 60 DEG C 1.25 extractum 2;

3rd step, mixes extractum 1 and extractum 2, adds the sodium chloride of 0.06% and the distilled water of 70 times of mixing extractum gross mass, and to add concentration be that 4% sodium hydroxide solution regulates PH to be 6.0, then add 0.2% active carbon and boil 10 minutes, filter, subpackage, sterilizing, obtains eye drop.

Model case one Cui, 43 years old, is engaged in agriculture, and because the busy farming season has no time to attend to board and lodging, has occurred eye socket distending pain, bitter taste in the mouth and dry throat in the recent period, ocular photophobia, with the phenomenon such as red blood streak, blurred vision.Remove examination in hospital: palpebral conjunctiva is slightly congested, bulbar conjunctiva mixing is dark congested, and conjunctival sac is sticky purulent secretion a little, corneal transparency, and pupil is little, responsive to luminous reflectance.Be diagnosed as liver and gall heated type keratitis on inspection.

Use the embodiment of the present invention 1 gained medicine, one day 3 times, each 2, use after 1 day, symptom disappears completely, and recovery from illness continues use one day consolidate curative effect.Patient is to the very satisfaction of this curative effect, and period has no adverse reaction, and drug withdrawal is not recurred so far.

Embodiment 2 embodiments provides a kind of medicine for the treatment of liver and gall heated type keratitis, is obtained by the raw medicinal material of following weight: Spica Prunellae 28g, Fructus prinsepiae utilis 10g, Fel Bovis seu Bubali 13g, Herba Lysimachiae 9g, Tibetan Artemisia anethoicles Mattf 24g, Herb Polygoni Chinensis 13g, Semen Celosiae 19g, Semen Abutili 8g, Herba Equiseti Hiemalis 22g, Radix Bupleuri 35g, Herba Dendranthematis indici 30g, Radix Angelicae Dahuricae 27g, Cortex Mori 15g, Radix Trichosanthis 11g, Concha Ostreae 20g, Flos Hibisci Mutabilis 28g, Cortex Fraxini 9g, Rhizoma Polygonati Verticillati 30g, Herba Hippuridis Vulgaris 12g and Cortex Moutan 29g.

Its preparation method comprises the following steps:

The first step, gets described crude drug and adds soak by water 2 times, and first time adds 4 times amount soak by water 1.5 hours, filters, and second time adds 3 times amount soak by water 1 hour, filters, and 2 filtrates is merged, and when concentrating under reduced pressure obtains 80 DEG C, relative density is the extractum 1 of 1.04, and medicinal residues are for subsequent use;

Second step, medicinal residues are added 60-90% soak with ethanol 0.5 hour, extract twice, each 1 hour, merge extractive liquid, 100 mesh filter screens filter, 7000 revs/min centrifugal, getting supernatant, is the ultrafiltration post ultrafiltration of 5000 through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 60 DEG C 1.25 extractum 2;

3rd step, mixes extractum 1 and extractum 2, adds the sodium chloride of 0.06% and the distilled water of 60 times of mixing extractum gross mass, and to add concentration be that 4% sodium hydroxide solution regulates PH to be 7.0, then add 0.2% active carbon and boil 10 minutes, filter, subpackage, sterilizing, obtains eye drop.

Model case two Zhu, 26 years old, welding worker, there is pain, photophobia in recent left eye, phenomenon of shedding tears, and with headache, pharyngeally dryly to itch.Remove hospital admission, check result cornea is shown in dendroid, fluorescein (+), and thick fur is yellow.Diagnostic result is liver and gall heated type keratitis.

The medicine of the use embodiment of the present invention 22 days, one day 3 times, each 2, liver and gall heated type keratitis symptom is improved a lot, left eye pain and headache phenomenon disappear, photophobia, to shed tears and pharyngeal dry itching alleviates, continue to use to the 3rd day, all transference cures, as usual depending on thing, recovery from illness, period has no adverse reaction, and drug withdrawal is not recurred so far.

Embodiment 3 embodiments provides a kind of medicine for the treatment of liver and gall heated type keratitis, is obtained by the raw medicinal material of following weight: Spica Prunellae 31g, Fructus prinsepiae utilis 17g, Fel Bovis seu Bubali 20g, Herba Lysimachiae 9g, Tibetan Artemisia anethoicles Mattf 25g, Herb Polygoni Chinensis 15g, Semen Celosiae 10g, Semen Abutili 11g, Herba Equiseti Hiemalis 19g, Radix Bupleuri 33g, Herba Dendranthematis indici 28g, Radix Angelicae Dahuricae 32g, Cortex Mori 17g, Radix Trichosanthis 7g, Concha Ostreae 19g, Flos Hibisci Mutabilis 16g, Cortex Fraxini 12g, Rhizoma Polygonati Verticillati 27g, Herba Hippuridis Vulgaris 12g and Cortex Moutan 27g.

Its preparation method comprises the following steps:

The first step, gets described crude drug and adds soak by water 2 times, and first time adds 6 times amount soak by water 2 hours, filters, and second time adds 5 times amount soak by water 1.5 hours, filters, and 2 filtrates is merged, and when concentrating under reduced pressure obtains 80 DEG C, relative density is the extractum 1 of 1.10, and medicinal residues are for subsequent use;

Second step, medicinal residues are added 60-90% soak with ethanol 1.5 hours, extract twice, each 2 hours, merge extractive liquid, 160 mesh filter screens filter, 10000 revs/min centrifugal, getting supernatant, is the ultrafiltration post ultrafiltration of 10000 through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 60 DEG C 1.25 extractum 2;

3rd step, mixes extractum 1 and extractum 2, adds the sodium chloride of 0.06% and the distilled water of 80 times of mixing extractum gross mass, and to add concentration be that 4% sodium hydroxide solution regulates PH to be 7.0, then add 0.2% active carbon and boil 10 minutes, filter, subpackage, sterilizing, obtains eye drop.

Long term toxicity test:

The medicine of the embodiment of the present invention 1 presses 10.78,20.35 and 33.48g crude drug/kg continuous use 16 weeks (1.0ml/100g body weight to mice, every day 2 times) and drug withdrawal after 4 weeks, result shows: the index such as hair, behavior, defecation, body weight, organ weights, hemogram, hepatic and renal function, blood glucose, blood fat of medicine of the present invention to mice all has no significant effect, internal organs naked eyes do not find that difference change and histological indications show, medication 16 weeks and drug withdrawal are after 4 weeks, and Organs of Mice is all without obviously changing.Illustrate that the medicine of the embodiment of the present invention 1 is little to toxicity after mice long-term prescription, also there is no difference reaction after drug withdrawal, application safety.

The medicine of the embodiment of the present invention 2 presses 10.78,20.35 and 33.48g crude drug/kg continuous use 16 weeks (1.0ml/100g body weight to mice, every day 2 times) and drug withdrawal after 4 weeks, result shows: the index such as hair, behavior, defecation, body weight, organ weights, hemogram, hepatic and renal function, blood glucose, blood fat of medicine of the present invention to mice all has no significant effect, internal organs naked eyes do not find that difference change and histological indications show, medication 16 weeks and drug withdrawal are after 4 weeks, and Organs of Mice is all without obviously changing.Illustrate that the medicine of the embodiment of the present invention 2 is little to toxicity after mice long-term prescription, also there is no difference reaction after drug withdrawal, application safety.

Hypersensitive test:

Get healthy albino guinea-pig 15, body weight 250-300g, male and female dual-purpose, reject both sides dorsal body setae before test, every side 2 × 2cm, is divided into test group, blank group (substrate) and positive controls at random.Positive controls DNFB, sensitization 1% concentration, excites and uses 0.1% concentration; The test group medicine of the embodiment of the present invention 3.The medicine getting the embodiment of the present invention 3 is applied in unhairing district, back, the next day 1 time, totally 3 times.In latter 14 days of last contact, at back, the same area smeared this product, at once observes after removing medicine after 6h, and in 24,48,72h observes with or without anaphylaxis.Blank group and positive controls excite by same method and contact and observe.Result of the test: namely positive controls animal skin occurs localized erythema and slight edema in sensitization after 1 hour, within 24 hours, symptoms of allergic increases the weight of, and have scleroma to be formed, sensitization rate is 100%, all there is not any symptoms of allergic in test group and blank treated animal skin, sensitization rate is 0%.

Clinical data

The clinical data of the medicine of embodiment 3 gained treatment liver and gall heated type keratitis:

Case selection: screen patient 120 example suffering from liver and gall heated type keratitis that in January, 2013 to 2014, year December Liang Suo hospital accepted for medical treatment as object of study.Be divided into two groups at random, often organize patient 60 people.Wherein one group uses the embodiment of the present invention 3 gained medicine, and matched group uses ribavirin eye drops, and the ordinary circumstance of two groups of patients, as the no difference of science of statistics such as age, the course of disease, has comparability.

Therapeutic Method:

Embodiment 3 medication therapy groups: use medicine prepared by the embodiment of the present invention 3, every day 3 times, each 22 days is 1 course for the treatment of.

Randomized controlled treatment group: every day 4 times, each 22 days is 1 course for the treatment of.

Curative effect determinate standard:

Cure: clinical symptoms all disappears, and vision is recovered completely, and fluorescein sodium corneal dyeing presents feminine gender;

Effective: clinical symptoms partial disappearance, vision is improved, and fluorescein sodium corneal dyeing presents the positive;

Invalid: clinical symptoms, vision is all without improvement, and fluorescein sodium corneal dyeing presents the positive.

Therapeutic outcome:

1, Comparison of therapeutic result:

After treating 1 course for the treatment of

Embodiment 3 medication therapy groups, totally 60 people, wherein 37 people cure, and 23 people are effective, and total effective rate reaches 100%;

Randomized controlled treatment group, totally 60 people, wherein 25 people cure, and 20 people are effective, and 15 people are invalid, and total effective rate reaches 75%;

After treating 2 courses for the treatment of

Embodiment 3 medication therapy groups, totally 60 people, wherein 59 people cure, and 1 people is effective, and total effective rate reaches 100%;

Randomized controlled treatment group, totally 60 people, wherein 46 people cure, and 5 people are effective, and 9 people are invalid, and total effective rate reaches 85%;

After treating 3 courses for the treatment of

Embodiment 3 medication therapy groups, totally 60 people, wherein 60 people cure, and total effective rate reaches 100%;

Randomized controlled treatment group, totally 60 people, wherein 46 people cure, and 5 people are effective, and 9 people are invalid, and total effective rate reaches 85%.

As can be seen here, the Drug therapy liver and gall heated type keratitis adopting the embodiment of the present invention 3 to prepare, relative to other drug on therapeutic effect, has significant improvement; The course for the treatment of significantly shortens.

According to the clinical statistics that above curative effect is stated, medicine provided by the invention have rapid-action, effective percentage is high, determined curative effect, safety are high, the advantage that has no side effect.

The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (5)

1. treat a medicine for liver and gall heated type keratitis, it is characterized in that, comprise following raw medicinal material: Spica Prunellae, Fructus prinsepiae utilis, Fel Bovis seu Bubali ,herba Lysimachiae, Tibetan Artemisia anethoicles Mattf, Herb Polygoni Chinensis ,semen Celosiae, Semen Abutili, Herba Equiseti Hiemalis, Radix Bupleuri, Herba Dendranthematis indici, the Radix Angelicae Dahuricae, Cortex Mori, Radix Trichosanthis, Concha Ostreae, Flos Hibisci Mutabilis, Cortex Fraxini, Rhizoma Polygonati Verticillati, Herba Hippuridis Vulgaris and Cortex Moutan.

2. medicine as claimed in claim 1, it is characterized in that, in described medicine, the parts by weight of each raw medicinal material are: Spica Prunellae 13-32 part, Fructus prinsepiae utilis 9-19 part, Fel Bovis seu Bubali 10-28 part, Herba Lysimachiae 6-17 part, hide Artemisia anethoicles Mattf 12-27 part, Herb Polygoni Chinensis 11-23 part, Semen Celosiae 8-22 part, Semen Abutili 7-15 part, Herba Equiseti Hiemalis 13-25 part, Radix Bupleuri 15-38 part, Herba Dendranthematis indici 14-32 part, Radix Angelicae Dahuricae 13-34 part, Cortex Mori 11-25 part, Radix Trichosanthis 6-16 part, Concha Ostreae 11-26 part, Flos Hibisci Mutabilis 13-30 part, Cortex Fraxini 7-18 part, Rhizoma Polygonati Verticillati 14-32 part, Herba Hippuridis Vulgaris 9-16 part and Cortex Moutan 13-31 part.

3. medicine as claimed in claim 1 or 2, it is characterized in that, in described medicine, the parts by weight of each raw medicinal material are: Spica Prunellae 25 parts, Fructus prinsepiae utilis 9 parts, Fel Bovis seu Bubali 12 parts, Herba Lysimachiae 7 parts, Tibetan Artemisia anethoicles Mattf 16 parts, Herb Polygoni Chinensis 21 parts, Semen Celosiae 11 parts, Semen Abutili 8 parts, Herba Equiseti Hiemalis 23 parts, Radix Bupleuri 32 parts, 29 parts, Herba Dendranthematis indici, the Radix Angelicae Dahuricae 32 parts, Cortex Mori 18 parts, Radix Trichosanthis 7 parts, Concha Ostreae 20 parts, Flos Hibisci Mutabilis 25 parts, Cortex Fraxini 11 parts, Rhizoma Polygonati Verticillati 30 parts, Herba Hippuridis Vulgaris 13 parts and Cortex Moutan 24 parts.

4. the medicine as described in as arbitrary in claims 1 to 3, it is characterized in that, in described medicine, the parts by weight of each raw medicinal material are: Spica Prunellae 28 parts, Fructus prinsepiae utilis 10 parts, Fel Bovis seu Bubali 13 parts, Herba Lysimachiae 9 parts, Tibetan Artemisia anethoicles Mattf 24 parts, Herb Polygoni Chinensis 13 parts, Semen Celosiae 19 parts, Semen Abutili 8 parts, Herba Equiseti Hiemalis 22 parts, Radix Bupleuri 35 parts, 30 parts, Herba Dendranthematis indici, the Radix Angelicae Dahuricae 27 parts, Cortex Mori 15 parts, Radix Trichosanthis 11 parts, Concha Ostreae 20 parts, Flos Hibisci Mutabilis 28 parts, Cortex Fraxini 9 parts, Rhizoma Polygonati Verticillati 30 parts, Herba Hippuridis Vulgaris 12 parts and Cortex Moutan 29 parts.

5. the medicine as described in as arbitrary in Claims 1-4, it is characterized in that, in described medicine, the parts by weight of each raw medicinal material are: Spica Prunellae 31 parts, Fructus prinsepiae utilis 17 parts, Fel Bovis seu Bubali 20 parts, Herba Lysimachiae 9 parts, Tibetan Artemisia anethoicles Mattf 25 parts, Herb Polygoni Chinensis 15 parts, Semen Celosiae 10 parts, Semen Abutili 11 parts, Herba Equiseti Hiemalis 19 parts, Radix Bupleuri 33 parts, 28 parts, Herba Dendranthematis indici, the Radix Angelicae Dahuricae 32 parts, Cortex Mori 17 parts, Radix Trichosanthis 7 parts, Concha Ostreae 19 parts, Flos Hibisci Mutabilis 16 parts, Cortex Fraxini 12 parts, Rhizoma Polygonati Verticillati 27 parts, Herba Hippuridis Vulgaris 12 parts and Cortex Moutan 27 parts.