CN105284912A - Preparation method of passion flower extract inhibiting pseudo-ginseng root rot - Google Patents
Preparation method of passion flower extract inhibiting pseudo-ginseng root rot Download PDFInfo
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- CN105284912A CN105284912A CN201510873307.7A CN201510873307A CN105284912A CN 105284912 A CN105284912 A CN 105284912A CN 201510873307 A CN201510873307 A CN 201510873307A CN 105284912 A CN105284912 A CN 105284912A
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Abstract
The invention relates to a preparation method of a passion flower extract inhibiting pseudo-ginseng root rot, belonging to the technical field of agricultural planting. The preparation method comprises the following steps: (1) selecting fresh passion flower leaves without diseases and insect pests; (2) cutting into pieces; (3) immersing the passion flower leaves into an extracting solution for soaking; (4) putting the passion flower leaves extracted at normal temperature into an ultrasonic instrument, and ultrasonically extracting; (5) after extracting, puring out, and filtering by using silk and filter paper; (6) performing vacuum concentration by using a vacuum decompression concentration pot; (7) performing ultraviolet sterilization; (8) after sterilization, adding sterile water to a constant volume, and putting into a 4-DEG C refrigerator for standby application. The method is simple, and convenience and quickness are realized; according to the method, passion flowers, especially the passion flower leaves, are taken as a raw material to extract the extracting liquid for inhibiting pseudo-ginseng root rot, and are used for preventing and treating pseudo-ginseng root rot; convenience and quickness are realized, the use of chemical agents can be avoided, and the pseudo-ginseng root rot can be effectively inhibited.
Description
Technical field
The present invention relates to a kind of preparation method suppressing the Passion flower P.E of pseudo-ginseng root rot, specifically to have passionflower and blade thereof for raw material, belong to passionflower processing technique field.
Background technology
Pseudo-ginseng (formal name used at school: Panaxpseudo-ginsengvar.notoginseng) is also known as radix notoginseng, pseudo-ginseng, Sedum uizoon, blood mountain grass, pouring in June, nilgiri nettle, and being the mutation of Araliaceae Panax panicled fameflower root, is the main component of Yunnan Baiyao.Main product ground is in the Yunnan of China and remote mountains, Guangxi.Be Chinese distinctive rare traditional Chinese medicine, one of Ye Shi China medicinal and edible plant the earliest, pseudo-ginseng is just acknowledged as since ancient times has promoting blood circulation and removing blood stasis, detumescence ding-tong effect significantly, has the good reputation of " invaluable ", " southern part of the country god's grass ".Because branch divides three, be seven, therefore be called " pseudo-ginseng ", have another name called pseudo-ginseng, invaluable etc., ancient times is also known as clear ginseng, blood ginseng, Panax pseudoginseng, Typhonium flagelliforme (Lodd.) Blume, mountain paint, Radix Notoginseng etc.Excavating in season in winter in spring two because being everlasting, being divided into again " spring seven " and " winter seven ".Because pseudo-ginseng is all panax species, and its effective active matter higher than with more than ginseng, be therefore called " king in ginseng " by modern Chinese herbal medicine medicine scholar again.
Pseudo-ginseng is perennial root herbaceous plant, has a growth cycle every year.Usually, the pseudo-ginseng of more than 2 years has two summit of growths in a growth cycle, the reproduction peak in nourish and grow peak and the 8-October in 4-June.The germination temperature of Panax notoginseng seeds is 10-30 DEG C, and optimum temperature is 20 DEG C, and the resting stage of seed is 45d-60d, and seedling needs just can sprout through the low temperature treatment of a period of time in sleep procedure, and very responsive to the reaction of light; Tradition is thought needs natural lighting 30% to grow by normal growth, therefore pseudo-ginseng cool canopy has saying of " three one-tenth printing opacities, seventy percent covers shade "; Show according to current research, optimum pseudo-ginseng canopy light transmittance is 8%-12%, will be adversely affected more than the growth of 17% pseudo-ginseng.
Because pseudo-ginseng is to the particularity of environmental requirement, often easily multiple diseases occurs, wherein pseudo-ginseng root rot is that the researchs such as disease the most serious in pseudo-ginseng process of growth are thought, pseudo-ginseng butt rot is mainly by the coefficient result of multiple disease fungus.Sticky weight of soil is met in field, impeded drainage, soil insect are many, easily brings out this disease.Especially after biennial pseudo-ginseng is transplanted, water irregular or not in time, root is shrivelled to feel like jelly, and soil moisture is saturated, Gen Maoyi death by suffocation, germ invades susceptible disease.
Root rot claims root rot, chicken droppings rotten, smelly seven etc.This disease can occur to three, on quadrennial pseudo-ginseng annual, but mainly cause harm one, biennial pseudo-ginseng.During morbidity, overground part leaf look turns yellow, growth potential difference.During temperature height at noon at initial stage, leaf is slightly sagging, sooner or later still can recover.Dig out diseased plant, root is caught an illness and is become yellowish-brown or rot.Master, side, fibrous root can be fallen ill, in the majority with main root.And with rhizome portion Yang Changchu start to decay the most common.If only side root-rot, there is corresponding leaf flavescence look in acrial part sometimes.Later stage old complaint all becomes pitchy or canescence, sposh pulpous state, therefore claims " chicken droppings is rotten ", invades relevant with bacterium.The Fusarium oxysporum of the different extracts of passionflower to one of pseudo-ginseng Pathogens Causing Root Rot Disease is inhibited, inquires into Passion flower P.E to the depression effect of pseudo-ginseng pine root fungus, is intended to for pseudo-ginseng comprehensive control of disease provides reference.
Pseudo-ginseng has effect of scattered silt hemostasis, detumescence ding-tong, has obvious curative effects to the heart, cerebrovascular system disease, therefore pseudo-ginseng and pseudo-ginseng total saposins were mainly used in disease such as treatment coronary heart diseases and angina pectoris, cerebrovascular sequelae etc. in recent years.In addition the report of the agricultural chemicals of root rot is also had.But be all chemical agent, can be residual on pseudo-ginseng, or act on not obvious.The present invention is directed to the deficiency of pseudo-ginseng root rot treatment in the past, develop a kind of extract suppressing pseudo-ginseng root rot.
Summary of the invention
The object of the present invention is to provide a kind of preparation method suppressing the Passion flower P.E of pseudo-ginseng root rot, the passionflower extract that the method is prepared can suppress the generation of pseudo-ginseng root rot well, thus plays suppression pseudo-ginseng root rot function.
In order to achieve the above object, the technical solution adopted in the present invention is:
Suppress a preparation method for the Passion flower P.E of pseudo-ginseng root rot, comprise the steps:
(1) raw material: select fresh, without the passionflower blade of damage by disease and insect as raw material;
(2) cut into slices: be cut into the sheet that 2 ~ 3cm is long;
(3) lixiviate: above-mentioned passionflower blade is immersed in extract and soaks 1h;
(4) ultrasonic extracts: the passionflower blade extracted through normal temperature puts into ultrasonic instrument, and ultrasonic extracts;
(5) filter: after extraction, pour out, with silk, Filter paper filtering;
(6) reduced pressure concentration: carry out reduced pressure concentration with vacuum decompressioning and concentrating tank;
(7) ultraviolet disinfection: concentrate is put into superclean bench and carries out ultraviolet sterilization;
(8) constant volume is preserved: add sterile water after sterilizing, constant volume, low temperature is for subsequent use; Step (1) described raw material be select fresh, without the passionflower blade of damage by disease and insect; Step (3) immerses in specific extraction liquid 8 times of water gagings, ethanol, ethyl acetate and soaks 1h; Ultrasonic instrument described in step (4), ultrasonic power 1000W, temperature 40 DEG C, extracts twice.
Tool of the present invention has the following advantages:
To the west of the present invention, a kind lotus leaf sheet is raw material, extracts passionflower leaf extract by ultrasonic; Suppress pseudo-ginseng root rot with passionflower leaf extract, this extracting method is simple, convenient and swift, and can avoid the use of chemical agent; There is good economic benefit prospect, effectively can suppress the generation of pseudo-ginseng root rot.
Embodiment
Be described in further details the present invention below by embodiment, these embodiments are only used for the present invention is described, do not limit the scope of the invention.
embodiment 1
Suppress a preparation method for the Passion flower P.E of pseudo-ginseng root rot, comprise the steps:
(1) raw material: select fresh, without 100, the passionflower blade of damage by disease and insect;
(2) cut into slices: be cut into the sheet that 2 ~ 3cm is long;
(3) lixiviate: above-mentioned passionflower blade is immersed in 8 times of water gagings and soaks 1h;
(4) ultrasonic extracts: the passionflower blade extracted through normal temperature puts into ultrasonic instrument, ultrasonic power 1000W, temperature 40 DEG C, extracts twice;
(5) filter: after extraction, pour out, with silk, Filter paper filtering;
(6) reduced pressure concentration: carry out reduced pressure concentration with vacuum decompressioning and concentrating tank;
(7) ultraviolet disinfection: concentrate is put into superclean bench and carries out ultraviolet sterilization;
(8) constant volume is preserved: add sterile water after sterilizing, the solution of constant volume 5g/ml concentration, be put into 4 DEG C of refrigerators for subsequent use.
embodiment 2
Suppress a preparation method for the Passion flower P.E of pseudo-ginseng root rot, comprise the steps:
(1) raw material: select fresh, without 100, the passionflower blade of damage by disease and insect;
(2) cut into slices: be cut into the sheet that 3cm is long;
(3) lixiviate: above-mentioned passionflower leaf sections is immersed in 8 times amount ethanol and soaks 1h;
(4) ultrasonic extracts: the passionflower blade extracted through normal temperature puts into ultrasonic instrument, ultrasonic power 1000W, temperature 40 DEG C, extracts twice;
(5) filter: after extraction, pour out, with silk, Filter paper filtering;
(6) reduced pressure concentration: carry out reduced pressure concentration with vacuum decompressioning and concentrating tank;
(7) ultraviolet disinfection: concentrate is put into superclean bench and carries out ultraviolet sterilization;
(8) constant volume is preserved: add sterile water after sterilizing, the solution of constant volume 5g/ml concentration, be put into 4 DEG C of refrigerators for subsequent use.
embodiment 3
Suppress a preparation method for the Passion flower P.E of pseudo-ginseng root rot, comprise the steps:
(1) raw material: select fresh, without 100, the passionflower blade of damage by disease and insect;
(2) cut into slices: be cut into the thin slice that 2.5cm is long;
(3) lixiviate: above-mentioned passionflower leaf sections is immersed in 8 times amount ethyl acetate and soaks 1h;
(4) ultrasonic extracts: the passionflower blade extracted through normal temperature puts into ultrasonic instrument, ultrasonic power 1000W, temperature 40 DEG C, extracts twice;
(5) filter: after extraction, pour out, with silk, Filter paper filtering;
(6) reduced pressure concentration: carry out reduced pressure concentration with vacuum decompressioning and concentrating tank;
(7) ultraviolet disinfection: concentrate is put into superclean bench and carries out ultraviolet sterilization;
(8) constant volume is preserved: add sterile water after sterilizing, the solution of constant volume 5g/ml concentration, be put into 4 DEG C of refrigerators for subsequent use.
embodiment 4
Suppress a preparation method for the Passion flower P.E of pseudo-ginseng root rot, comprise the steps:
(1) raw material: select fresh, without 100, the passionflower blade of damage by disease and insect;
(2) cut into slices: be cut into the sheet that 2.6cm is long;
(3) lixiviate: above-mentioned passionflower leaf sections is immersed in 8 times of water gagings and soaks 1h;
(4) ultrasonic extracts: the passionflower blade extracted through normal temperature puts into ultrasonic instrument, ultrasonic power 1000W, temperature 40 DEG C, extracts twice;
(5) filter: after extraction, pour out, with silk, Filter paper filtering;
(6) reduced pressure concentration: carry out reduced pressure concentration with vacuum decompressioning and concentrating tank;
(7) ultraviolet disinfection: concentrate is put into superclean bench and carries out ultraviolet sterilization;
(8) constant volume is preserved: add sterile water after sterilizing, the solution of constant volume 5g/ml concentration, be put into 4 DEG C of refrigerators for subsequent use.
Claims (4)
1. suppress a preparation method for the Passion flower P.E of pseudo-ginseng root rot, it is characterized in that: comprise the following steps: (1) raw material is selected: select fresh, without the passionflower blade of damage by disease and insect as raw material; (2) cut into slices: be cut into the sheet that 2 ~ 3cm is long; (3) lixiviate: above-mentioned passionflower blade is immersed in extract and soaks 1h; (4) ultrasonic extracts: the passionflower blade after normal temperature soak extraction puts into ultrasonic instrument, and ultrasonic extracts; (5) filter: after extraction, pour out, with silk, Filter paper filtering; (6) reduced pressure concentration: carry out reduced pressure concentration with vacuum decompressioning and concentrating tank; (7) ultraviolet disinfection: concentrate is put into superclean bench and carries out ultraviolet sterilization; (8) constant volume is preserved: add sterile water after sterilizing, the solution of constant volume 5g/ml concentration, be put into 4 DEG C of refrigerators for subsequent use.
2. a kind of preparation method suppressing the Passion flower P.E of pseudo-ginseng root rot according to claim 1, is characterized in that: step (1) described raw material be select fresh, without the passionflower blade of damage by disease and insect.
3. a kind of preparation method suppressing the Passion flower P.E of pseudo-ginseng root rot according to claim 1, is characterized in that: the extract described in step (3) is water, ethanol or ethyl acetate.
4. a kind of preparation method suppressing the Passion flower P.E of pseudo-ginseng root rot according to claim 1, is characterized in that: the condition that the ultrasonic described in step (4) extracts is: ultrasonic power 1000W, temperature 40 DEG C, extracts twice.
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| CN201510873307.7A CN105284912A (en) | 2015-12-03 | 2015-12-03 | Preparation method of passion flower extract inhibiting pseudo-ginseng root rot |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106342920A (en) * | 2016-08-25 | 2017-01-25 | 文山苗乡三七科技有限公司 | Method for preventing and controlling disease damage and pest damage of pseudo-ginseng by virtue of no- residue farm chemicals |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106342920A (en) * | 2016-08-25 | 2017-01-25 | 文山苗乡三七科技有限公司 | Method for preventing and controlling disease damage and pest damage of pseudo-ginseng by virtue of no- residue farm chemicals |
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Application publication date: 20160203 |
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