CN105284870A - Insecticide composition of ivermectin and phoxim - Google Patents

Abstract

The invention belongs to the technical field of pesticides and insecticides, and particularly relates to an insecticide composition of ivermectin and phoxim. The insecticide composition is characterized by comprising the following raw materials by weight: 20 percent of ivermectin and phoxim, 5.0 to 15.0 percent of an emulsifier, and 65.0 to 75.0 percent of a filler carrier. The insecticide composition adopting the two frequently-used medicaments as main ingredients and further adopting the emulsifier and the filler carrier has the advantage that advantage complementation is realized; the process is simple; all the raw materials are converted into the product; the yield is high; the generation of three wastes is avoided; the preventing and treating effects on palm thrips are improved effectively.

Description

A kind of insecticidal composition of ivermectin and phoxim

technical field

The invention belongs to the technical field of pesticides and insecticides, and in particular relates to an insecticidal composition of ivermectin and phoxim.

Background technique

Thrips palmi belongs to Thysanoptera, Thripidae, is a worldwide pest. The pest has a wide range of hosts and likes to eat various vegetables such as Solanaceae, Cucurbitaceae, and Legumes. In addition to causing direct damage to host plants, Thrips palmi can also transmit various viral diseases of the tomato spotted wilt virus in a persistent manner, causing heavy losses to vegetable production. Thrips palmi is small and insidious. With the enhancement of drug resistance, it is difficult to be effectively controlled by general chemical agents.

At present, the medicaments used to control the pests in production are generally spinosad, imidacloprid, beta-cypermethrin, cyhalothrin and other compound medicaments, but the control effect is not ideal. Due to the long-term repeated or unscientific use of these agents, the resistance of Thrips palmi is serious, and the dosage is increased, which leads to a substantial increase in the control cost.

Ivermectin, commonly known as Ivermectin in English, is a semi-synthetic macrolide multi-component antibiotic produced by the fermentation of Streptomyces avermitilis. The main components are ivermectin Bla and ivermectin B1b. The chemical name of ivermectin B1a is "5-O-dimethyl-22,23-dihydroabamectin". The name is "5-O-Dimethyl-25-bis(1-methylpropyl)-22,23-dihydro-25-(1-methylethyl) Abamectin". Its structural formula is as follows:

Phoxim, commonly known as Phoxim in English, is an organophosphorus insecticide. Its chemical name is "O,O-ethyl-O-(phenylacetonitrile ketoxime) phosphorothioate". The structural formula is as follows:

At present, there is no insecticidal composition compounded with ivermectin and phoxim on the market. For the above reasons, it is particularly important to invent a new type of pesticide composition.

Contents of the invention

The purpose of the present invention is to provide an insecticidal composition with high efficiency, low toxicity and safe use. The insecticidal composition has a very significant synergistic effect on vegetable thrips palmi, and can also be used for the control of whitefly, aphid and other thrips.

A kind of insecticidal composition of ivermectin and phoxim provided by the invention, in terms of raw material weight percentage, its raw material is composed of: ivermectin and phoxim account for 20% of the total weight, emulsifier is 5.0% -15.0%, the filler carrier is 65.0-75.0%, wherein the weight ratio of ivermectin and phoxim is 1.0-8.0:19.0-12.0.

In the present invention, the insecticidal mechanism of the ivermectin is: increase the release of pest inhibitory transmitter gamma-aminobutyric acid (GABA), and open the Cl ion channel controlled by glutamic acid, and enhance the nerve membrane to Cl ion channel. The permeability of ions, thereby blocking the transmission of nerve signals, causing the muscle cells of pests to lose their ability to contract, resulting in the death of the insect body. The agent has contact killing and stomach poisoning effects, and has the characteristics of high efficiency, low toxicity, low residue, and pollution-free biological agents. moths, cabbage caterpillars, etc.), Diptera (Liriomyza sativae, etc.), Hemiptera (aphids, psyllids, etc.) pests and mites (tetranychus cinnabarinus, Tetranychus urticae, etc.) It can be used for comprehensive control of thrips palmi, an agricultural pest. However, when the agent is used alone, the pests are likely to develop drug resistance, thereby significantly reducing the cost performance of the recalcitrant pest Thrips palmi.

In the present invention, the insecticidal mechanism of phoxim is as follows: as an acetylcholinesterase inhibitor, it acts on acetylcholinesterase (AChE) in the central nervous system of pests. By inhibiting the activity of AChE, the acetylcholine in the synaptic gap cannot be decomposed, and the post-synaptic membrane is constantly stimulated, causing the insects to show symptoms of excitement, and finally paralyzed and died. The agent has low toxicity and has contact and stomach poisoning effects. etc.) pests and mites (Tetranychus cinnabarinus, Tetranychus urticae, etc.) have good control effects, and can also be used for comprehensive management of agricultural pests Thrips palmi. In recent years, it has been found in agricultural production that when this agent is used alone, pests are prone to develop resistance, which significantly reduces the cost-effectiveness of the recalcitrant pest Thrips palmi.

In the present invention, since the insecticidal mechanisms of ivermectin and phoxim are different when the two substances are combined, the compound composition of the two agents can act on different sites of the target at the same time, play a role of complementary advantages, and effectively improve Control effect against Thrips palmi.

The emulsifier described in the present invention is a compound agricultural emulsifier, containing Ning milk 34 ^# (purchased from Shandong Luke Chemical Group Co., Ltd.), thick milk 500 ^# (purchased from Tianjin Shengtongtai Chemical Co., Ltd.), thick milk 0606 ^# (purchased from Shandong Luke Chemical Group Co., Ltd.), its percentage by weight is: Ningru 34 ^# 50%; Concentrated milk 500 ^# 30%; Concentrated milk 0606 ^# 20%.

The filler carrier of the present invention is xylene, which can be widely used in industrial production because of its best dissolution effect.

In the present invention, ivermectin and phoxim are 1/19, 2/18, 3/17, 4/16, 5/15, 6/14, 7/13, 8/12, 9 /11, 10/10 compound, with the mass fraction of 10% imidacloprid WP and clear water as the control, the indoor toxicity test was carried out, and the co-toxicity coefficient to Thrips palmi was 95.16-191.38 after 48 hours of medicine. The results showed that the compound preparation Compared with a single dose, it has a significant synergistic effect, among which 2/18 and 3/17 have the most prominent effects, and their co-toxicity coefficients to Thrips palmi are 190.91 and 191.38 respectively at 48 hours after administration. For specific steps and results, please refer to the indoor joint toxicology test.

Indoor combined toxicity assay:

Use the leaf dipping method. The test agent is 92.40% ivermectin original drug (produced by Zhejiang Hisun Chemical Co., Ltd.), 87.00% phoxim original drug (produced by Shandong Dacheng Agrochemical Co., Ltd.) and a compound composed of the two original drugs in proportion things. The bioassay method is an improved leaf tube drug film method based on Alfredo and Anthony's TIBS method. Dissolve the test agent in DMF first, dilute it with 0.05% Tween-80 to 7-8 serial concentrations on the basis of the preliminary test, fill 1.5mL centrifuge tubes respectively, seal and place for 4 hours, pour out the drug solution, and let it dry for a while. Use; use a 1cm diameter hole puncher to punch fresh cabbage leaves into leaf discs, soak the medicine in the medicinal solution for 10 seconds, place the leaves on absorbent paper to dry, and clamp them into a centrifuge tube of the corresponding concentration of medicinal solution with tweezers. 1 tablet per tube; each concentration was repeated 4 times, with 0.05% Tween-80 dipped tubes and soaked leaves as blank control; the adult thrips palmi to be tested was sucked into the centrifuge tube with an insect sucker, and 20-23 heads were repeated for each repetition. Cover the centrifuge tube and place it in a light incubator at (25±1)°C, relative humidity (70±5)%, light L:D=16:8, and check the dead number of Thrips palmi after 48h to determine If the tip of the brush lightly touches the worm body and cannot crawl, it is considered dead.

Data processing method:

Use SPSS to calculate the LC50 of each treatment, and use Sun Yunpei's method to calculate the co-toxicity coefficient (CTC) of the pharmaceutical composition:

Co-toxicity coefficient (CTC) = [mixture actual toxicity index (ATI) ÷ mixture theoretical toxicity index (TTI)] × 100

Mixture Actual Toxicity Index (ATI) = (LC50 of standard drug ÷ LC50 of mixture) × 100

Mixture theoretical toxicity index (TTI) = toxicity index (TI) of component A × content of A in the active ingredient of the mixture + toxicity index (TI) of component B × content of B in the active ingredient of the mixture ( In the present invention, A is ivermectin, B is phoxim)

Toxicity index (TI) = (LC50 of standard drug ÷ LC50 of test drug) × 100

When the co-toxicity coefficient is significantly greater than 100, it indicates that there is a synergistic effect; when it is close to 100, it indicates that there is an additive effect; and when it is significantly less than 100, it indicates that there is an antagonistic effect.

test results:

Indoor joint toxicity assay results (table 1) show that ivermectin and phoxim compound, when ivermectin and phoxim weight ratio are respectively 1-8: 19-12, 48h after the drug is harmful to palm The co-toxicity coefficient (CTC) of thrips is 127.13-191.38, which has obvious synergistic effect.

Table 1 Indoor joint toxicological determination of ivermectin·phoxim on Thrips palmi

Test drug Component ratio LC50(ppm) T.I. ATI TTI CTC Ivermectin + phoxim 1:19 27.114 -- 79.64 53.24 149.59 Ivermectin + phoxim 2:18 20.257 -- 106.60 55.70 191.38 Ivermectin + phoxim 3:17 19.448 -- 111.03 58.16 190.91 Ivermectin + phoxim 4:16 20.108 -- 107.39 60.62 177.15 Ivermectin + phoxim 5:15 20.633 -- 104.66 63.08 165.90 Ivermectin + phoxim 6:14 23.310 -- 92.64 65.54 141.34 Ivermectin + phoxim 7:13 22.842 -- 94.54 68.01 139.01 Ivermectin + phoxim 8:12 24.104 -- 89.59 70.47 127.13 Ivermectin + phoxim 9:11 31.116 -- 69.40 72.93 95.16 Ivermectin + phoxim 10:10 29.102 -- 74.20 75.39 98.42 Ivermectin -- 21.594 100.00 -- -- -- Phoxim -- 42.527 50.78 -- -- --

According to the indoor joint toxicology test, determine the optimal composition ratio and the best composition ratio as follows:

The preferred composition ratio is:

The optimal composition ratio is:

A kind of insecticidal composition of ivermectin and phoxim according to the present invention, its preparation method is: under stirring condition, ivermectin is dissolved with filler carrier, then add phoxim in proportion, emulsify After fully stirring, let it stand still, and after passing the inspection, it will be packaged as required to be the product.

In summary, a kind of insecticidal composition of ivermectin and phoxim according to the present invention adopts two commonly used medicaments as main components, and is equipped with emulsifier and filler carrier to achieve complementary advantages and complement each other. role. The process is simple, all input raw materials are converted into products, the yield is high, no three wastes are generated, and the control effect on thrips palmi is effectively improved. At the same time, the insecticidal composition has the characteristics of high efficiency and low toxicity.

detailed description

In this experiment, all the examples were carried out on the peppers in the solar greenhouse of Xizhen Village, Yuhuangmiao Subdistrict Office, Shanghe County, Jinan City, Shandong Province, and the variety of the peppers tested was 'Hongluodan'. The test treatment is respectively 90mL and 60mL per mu of the insecticides obtained in Example 1, Example 2, and Example 3, and 40g of 10% imidacloprid WP (produced by Zhejiang Haizheng Chemical Co., Ltd.) as a standard control with a mass fraction. With sprayed water as the blank control, a total of 8 treatments, each treatment repeated 4 times, a total of 32 plots, each with an area of 40m ² . On May 16, 2015, the drug was sprayed once, and the drug device was a backpack-type Singapore Linong sprayer. The water consumption is 60kg/667m ² . Investigate the base number of insect population before the drug treatment, and investigate the number of residual live insects 1, 3, and 7 days after the drug treatment. Survey method: Sampling at 5 points in each plot, fixing 4 pepper plants at each point, randomly selecting 5 flowers on each pepper plant, and conducting each survey between 6:00 and 7:30 in the morning, and recording the life of Thrips palmi on it. bug count. Calculate the population reduction rate and correct the control effect.

Data processing method:

Insect population decline rate (%) = (Insect population base before treatment - number of living insects after treatment) ÷ Insect population base before treatment × 100

Correct control effect (%)=(treatment area worm population decrease rate-control area worm population decrease rate) ÷ (100-control area worm population decrease rate) * 100 The present invention is further described with embodiment below:

Example 1:

The emulsifier in this embodiment is a compound agricultural emulsifier, containing Ning milk 34 ^# (purchased from Shandong Luke Chemical Group Co., Ltd.), thick milk 500 ^# (purchased from Tianjin Shengtongtai Chemical Co., Ltd.), thick milk 0606 ^# ( Purchased from Shandong Luke Chemical Group Co., Ltd.), its percentage by weight is: Ningru 34 ^# 50%; Concentrated milk 500 ^# 30%; Concentrated milk 0606 ^# 20%.

The filler carrier in this example is xylene.

test

Result of the test (table 2) shows that embodiment 1 insecticide EC mu uses 90mL, 60mL and all has better control effect to western flower thrips, and the corrected control effect of 7d after the medicine is respectively 96.44%, 92.79%, significantly superior. effect on the standard control.

Table 2 Field control effect of ivermectin·phoxim embodiment 1 on Thrips palmi

Example 2:

The emulsifier in this embodiment is a compound agricultural emulsifier, containing Ning milk 34 ^# (purchased from Shandong Luke Chemical Group Co., Ltd.), thick milk 500 ^# (purchased from Tianjin Shengtongtai Chemical Co., Ltd.), thick milk 0606 ^# ( Purchased from Shandong Luke Chemical Group Co., Ltd.), its percentage by weight is: Ningru 34 ^# 50%; Concentrated milk 500 ^# 30%; Concentrated milk 0606 ^# 20%.

The filler carrier in this example is xylene.

Result of the test (table 3) shows that embodiment 2 insecticide EC mu uses 90mL, 60mL and all has better control effect to western flower thrips, and the corrected control effect of 7d after the medicine is respectively 95.62%, 92.48%, significantly superior. effect on the standard control.

Table 3 Field control effect of ivermectin·phoxim embodiment 2 on Thrips palmi

Example 3:

The emulsifier in this embodiment is a compound agricultural emulsifier, containing Ning milk 34 ^# (purchased from Shandong Luke Chemical Group Co., Ltd.), thick milk 500 ^# (purchased from Tianjin Shengtongtai Chemical Co., Ltd.), thick milk 0606 ^# ( Purchased from Shandong Luke Chemical Group Co., Ltd.), its percentage by weight is: Ningru 34 ^# 50%; Concentrated milk 500 ^# 30%; Concentrated milk 0606 ^# 20%.

The filler carrier in this example is xylene.

Result of the test (table 4) shows that embodiment 3 insecticide EC mu uses 90mL, 60mL and all has better control effect to western flower thrips, and the corrected control effect of 7d after the medicine is respectively 92.36%, 91.60%, significantly superior. effect on the standard control.

Table 4 The field control effect of ivermectin·phoxim embodiment 3 on Thrips palmi

Claims (8)

1. a Pesticidal combination for ivermectin and phoxim, is characterized in that: by raw material weight percentages, and its raw material consists of: ivermectin and phoxim account for 20% of composition total weight, and emulsifier is 5.0-15.0%, and filling carrier is 65.0-75.0%.

2. Pesticidal combination according to claim 1, is characterized in that: the weight ratio of ivermectin and phoxim is 1.0-8.0:19.0-12.0.

3. Pesticidal combination according to claim 1, is characterized in that: the weight ratio of ivermectin and phoxim is 2.0-3.0:18.0-17.0.

4. Pesticidal combination according to claim 1, is characterized in that: described emulsifier is composite farm emulsifier, and percentage by weight is: peaceful breast 34 ^#50%; Dense newborn 500 ^#30%; Dense newborn 0606 ^#20%.

5. Pesticidal combination according to claim 1, is characterized in that: described filling carrier is dimethylbenzene.

6. Pesticidal combination according to claim 1, is characterized in that: composition and percentage by weight as follows:

。

7. Pesticidal combination according to claim 1, is characterized in that: described Pesticidal combination is for preventing and treating vegetables palm thrips.

8. Pesticidal combination according to claim 1, is characterized in that: described Pesticidal combination also can be used for the control of aleyrodid, aphid and other thrips.