CN105273998A - Double-shielding box-type DNA sequencer with portal - Google Patents
Double-shielding box-type DNA sequencer with portal Download PDFInfo
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- CN105273998A CN105273998A CN201510726802.5A CN201510726802A CN105273998A CN 105273998 A CN105273998 A CN 105273998A CN 201510726802 A CN201510726802 A CN 201510726802A CN 105273998 A CN105273998 A CN 105273998A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The invention relates to a double-shielding box-type DNA sequencer with a portal. The double-shielding box-type DNA sequencer comprises a reaction record subsystem, a reagent supply system, a control system and a sequencer box bearing the systems, the reaction record subsystem comprises a reaction bin component, a movement platform component, a movement control unit, a CCD camera and a platform supporting component, a reaction chip component is arranged in the reaction bin component, and reaction liquid enters a reaction chip for chemical reaction to generate visible light under action of the control system. The double-shielding box-type DNA sequencer comprises the portal and a turnable reaction bin double-shielding structure, the reaction chip can be loaded under double protection, and the portal itself has extremely high stability; after long-time use, the portal does not deform, and the CCD camera can be stabilized at a same point for testing.
Description
Technical field
The present invention relates to DNA sequencing field, particularly relate to one and there is body structure, be easy to carry, and can the dual box DNA sequencer of screening with door frame of autonomous feed flow.
Background technology
In DNA sequencing technical field, integrated operation flow process is described below: DNA sample is by after fragmentation, application is built storehouse reagent and is carried out adding joint, strand is caught, be bonded to microballoon, microemulsion pcr amplification, breakdown of emulsion liquid, obtain the DNA library be based upon on microballoon, application adds model by laies such as the enzymes of library and sequencing reaction needs to the sequence testing chip with micro reaction pool, sequence testing chip and sequencing reagent are mounted on main frame, order-checking program is started according to module number and position by computer for controlling, sequencing reaction is carried out in automatization, the data transmission produced is to Data Analysis Computer, after completing order-checking, computation analysis software carries out image procossing, sequence reads, mass analysis, the work such as sequence assembly, finally obtain the sequence information of DNA sample.
Stable, airtight operating environment should be had at the sequencing reaction of reality and agent delivery device, especially should have handled easily and the characteristic of integrated movable to the relevant device of sequencing reaction, reaction chip is arranged in sequencing reaction equipment to be needed to be set in stable and is reserved with the position in space of enough taking pictures; Existing DNA sequencing equipment is difficult to meet the demand.
In view of above-mentioned defect, creator of the present invention obtains this creation finally through long research and practice.
Summary of the invention
The object of the present invention is to provide a kind of dual box DNA sequencer of screening with door frame, in order to overcome above-mentioned technological deficiency.
For achieving the above object, the invention provides a kind of dual box DNA sequencer of screening with door frame, it comprises reacting record sub-system, agent delivery system and Controlling System, and carry the sequenator casing of above-mentioned each system, wherein, described reacting record sub-system and agent delivery system are separately positioned in the interval, the left and right sides of sequenator casing; The rear side of the reacting record sub-system of described set-up of control system on the left of casing in interval, control reaction sub-system and agent delivery system according to predetermined sequential action;
Described reacting record sub-system comprises reaction warehouse assembly, motion platform assembly, motion control unit, CCD camera and platform supporting component, described reaction warehouse assembly, reaction chip assembly is set in it, under the effect of Controlling System, reaction solution enters in reaction chip and carries out chemical reaction, produces visible ray; Described motion platform assembly, CCD camera is fed into suitable picture-taking position by it, to take pictures collection to the visible ray information produced in reaction chip to make CCD camera; Described platform assembly, its support described in reaction warehouse assembly, motion platform assembly;
Described reaction warehouse assembly comprises the door frame of the vertical setting be arranged on described platform assembly, the lower end of described door frame is fixedly connected with platform assembly, and be arranged on the front end of platform assembly, on rear side of door frame, be provided with described CCD camera;
Install by reaction chip in the middle of described reaction warehouse assembly;
Form curved cavity in the one side of door frame, turning reaction warehouse assembly is installed in cavity, and the turnable basement be rotatably connected with turning reaction warehouse assembly.
Further, described door frame is the structure that two sides have sidepiece baffle plate, and it is one-body molded;
Described turnable basement is fixed on the middle part of door frame.
Further, described sequenator casing comprises body supports assembly, is arranged on the interior lining panel assembly inside body supports assembly;
Casing is divided into left and right two portions, is provided with reacting record sub-system in the casing of left side; In right case, be provided with agent delivery system, comprise pump valve pipe integrated unit, liquid case lifting gantry and indicating meter, the rear side of casing is provided with control unit.
Further, described body supports assembly is made up of steel plate splicing, is fixed by the mode of splicing or screw fastening; It comprises base assembly, top plate member, connects and composes the support frame assembly of casing side frame with base assembly, buffering system mount pad;
Wherein, described support frame assembly comprises left side vertical plate assembly, middle the riser assembly separating left and right two tanks, right side riser assembly and is arranged on the right side benefit slab of right side riser forward portion, side stand rearways board component, is arranged on the first cable permanent seat in top plate member and the first cable permanent seat, and by web plate that each assembly links together.
Further, be also provided with electronic lock assembly in the left side of the upper end of described door frame, described electronic lock assembly comprises electronics lock and inductive switch, and described inductive switch is arranged on electronics lock, and electronics lock is arranged on the sidepiece of turning warehouse;
Electronics lock comprises sidepiece mounting plate, and sidepiece mounting plate offers open holes;
Electronic lock is done to be fixed on turning warehouse by open holes and mounting screw;
Be provided with the first symmetrical framework and the second framework in described sidepiece mounting plate side, leave the space of installing inductive switch in the centre of the first framework and the second framework.
Further, described turning reaction warehouse assembly comprises turning warehouse, in the inner side of the centre of described turning warehouse, reaction chip assembly is installed, accordingly, the first cavity holding reagent pipeline is provided with in the centre of turning warehouse, the bottom of the first cavity is provided with the first boss, and the first boss is in order to carry the strut member of described reaction chip assembly.
Further, described agent delivery system comprises control unit, damping fluid case loading/unloading platform assembly, liquid case lifting gantry assembly, pump valve integrated pipe assembly, wherein,
Described damping fluid case loading/unloading platform can load and unload all ingredients, and be transported to the position of specifying up and down through liquid case lifting gantry assembly, for described pump valve integrated pipe Component driver, the Reagent Tube that described pump valve integrated pipe component selection needs, through the driving of hydro-pump and the open and close controlling of hydraulicvalve, the reagent delivery of specifying is reacted to reaction warehouse assembly.
Compared with prior art beneficial effect of the present invention is: the present invention has the dual box DNA sequencer of screening of door frame, comprise door frame and the dual shielding structure of turning reaction warehouse, not only can load reaction chip under double protection, door frame itself has high stability; After long-term use, door frame is indeformable, and CCD camera can be stabilized in same point and test.
In the process of DNA sequencing, sequenator should have continuous, controlled characteristic in agent delivery, sequencing reaction process, and sequencing reaction and agent delivery device should have stable, airtight operating environment; One-piece construction is stablized, and order-checking efficiency is high.Reaction warehouse assembly can be installed, fix and reaction chip described in adjusting, ensure that reaction chip reacts in stable environment, and reaction chip can conveniently take out and lock.
The present invention arranges casing at the reaction warehouse assembly of DNA sequencing and the periphery of reagent assembly, makes reaction warehouse have enough precision, stability and clean space; Space rationally, be easy to carrying adjustment.
Casing is provided with two portions space, left and right, and hold reaction component and agent delivery, memory module respectively, the equipment in space, left and right works respectively, and form streamlined operation, testing efficiency is high.
The liquid-supplying system of DNA sequencer of the present invention controls the storage of reagent, conveying, selection by control unit; Liquid case lifting gantry assembly by guide rail movement by reagent delivery to specified location, it is convenient, precisely to control; Pump valve integrated pipe assembly and damping fluid case loading/unloading platform assembly transport by liquid case lifting gantry up and down, complete reagent conveying, reaction process.
Accompanying drawing explanation
Fig. 1 is the functional block diagram of DNA sequencer of the present invention;
Fig. 2 a is the one-piece construction schematic diagram of DNA sequencer casing of the present invention;
Fig. 2 b is the first structural representation of the body supports assembly of DNA sequencer casing of the present invention;
Fig. 2 c is the second structural representation of the body supports assembly of DNA sequencer casing of the present invention;
Fig. 3 is the one-piece construction schematic diagram of DNA sequencer reacting record sub-system of the present invention;
Fig. 4 is the first perspective view of reaction warehouse assembly of the present invention;
Fig. 5 a is the first perspective view of turning warehouse of the present invention;
Fig. 5 b is the second perspective view of turning warehouse of the present invention;
Fig. 6 a is the perspective view of preset pieces of the present invention;
Fig. 6 b is the structural representation of electronic lock of the present invention;
Fig. 6 c is the structural representation of electronics lock of the present invention;
Fig. 6 d is the sectional structure schematic diagram of electronic lock of the present invention;
Fig. 7 is the sectional structure schematic diagram of turning reaction warehouse assembly of the present invention along the first lug;
Fig. 8 is the 3rd perspective view of turning warehouse of the present invention;
Fig. 9 a is the structural representation of reaction chip assembly of the present invention;
Fig. 9 b is the structural representation of reaction chip mount pad of the present invention;
Fig. 9 c is the structural representation of the medial surface of reaction warehouse base of the present invention;
Fig. 9 d is the structural representation of the outer side of reaction warehouse base of the present invention;
Fig. 9 e is the structural representation of temperature control assembly cover plate of the present invention;
Figure 10 a is the sectional structure schematic diagram along liquid inlet and outlet of reaction chip assembly of the present invention;
Figure 10 b is the sectional structure schematic diagram of turning reaction warehouse assembly of the present invention along self-adaptative adjustment part;
Figure 11 is turning reaction warehouse assembly sectional structure schematic diagram of the present invention;
Figure 12 a is the sectional structure schematic diagram of back shroud of the present invention;
Figure 12 b is the perspective view of back shroud assembly of the present invention;
Figure 12 c is the perspective view of back shroud of the present invention;
Figure 13 a is the structural representation of platform assembly of the present invention;
Figure 13 b is the structural representation of vibration damper plate of the present invention;
Figure 14 is the structural representation of motion platform assembly of the present invention;
Figure 15 is the sectional structure schematic diagram of motion platform assembly of the present invention;
Figure 16 a is the perspective view of liquid case lifting gantry assembly of the present invention;
Figure 16 b is the side-looking structural representation of liquid case lifting gantry assembly of the present invention;
Figure 17 a is the perspective view of pump valve integrated pipe assembly of the present invention;
Figure 17 b is the plan structure schematic diagram of pump valve integrated pipe assembly of the present invention;
Figure 18 a is the perspective view of damping fluid case loading/unloading platform assembly of the present invention;
Figure 18 b be damping fluid case loading/unloading platform assembly of the present invention face structural representation.
Embodiment
Below in conjunction with accompanying drawing, to above-mentioned being described in more detail with other technical characteristic and advantage of the present invention.
Refer to shown in Fig. 1, it is the functional block diagram of DNA sequencer casing of the present invention, the DNA sequencer of the embodiment of the present invention comprises reacting record sub-system, agent delivery system and Controlling System, and carry the sequenator casing of above-mentioned each system, in the present embodiment, described reacting record sub-system and agent delivery system are separately positioned in the interval, the left and right sides of sequenator casing, and are connected by the agent delivery pipeline be arranged in casing; The rear side of the reacting record sub-system of described set-up of control system on the left of casing in interval, control reaction sub-system and agent delivery system according to predetermined sequential action.
Described Controlling System controls agent delivery system by reagent delivery in reacting record sub-system, reaction warehouse assembly is provided with in reacting record sub-system, reagent flows through the reaction chip be arranged in reaction warehouse assembly and reacts, and the CCD camera in reacting record sub-system gathers the photo in reaction process and obtains the DNA sequence dna surveyed by analysis afterwards.
Refer to shown in Fig. 2 a, it is the one-piece construction schematic diagram of DNA sequencer casing of the present invention, the sequenator casing of the embodiment of the present invention comprises body supports assembly 21, be arranged on the interior lining panel assembly inside body supports assembly 21, offside door assembly assembly 23, offside door assembly assembly 26, damping fluid case loading/unloading platform assembly 25, left cap board component 30, rear side cover board component, upper cover plate 3110.
Described interior lining panel assembly to comprise on the left of left side storehouse interior finishing panel assembly 24, storehouse, right side interior finishing panel assembly 22 on the right side of interior finishing panel assembly and storehouse, right side.
In embodiments of the present invention, casing is divided into left and right two portions, is provided with reacting record sub-system in the casing of left side, i.e. reaction warehouse assembly and CCD camera; In right case, be provided with agent delivery system, comprise pump valve pipe integrated unit, liquid case lifting gantry and indicating meter, the rear side of casing is provided with control unit.Operationally, reagent solution is delivered in reaction warehouse assembly by pump valve integrated unit in order, completes chemical reaction, and under the control of the control unit, CCD camera completes takes pictures, and eventually passes through data analysis and to take pictures result, point reaches a conclusion.
Wherein, the cutting part of the bottom of body supports assembly 21 supporting box, sidepiece, two housing area in rear portion and left and right; The medial support assembly of two housing area, left and right is provided with plaque, plays effect that is heat insulation and increase structural stability; Being provided with offside door assembly assembly 23 in the front portion of left side casing, when opening offside door assembly assembly, can the reaction warehouse assembly be arranged in left box body being operated; Be provided with offside door assembly assembly 26 on the right side of right side casing, when opening, can operate pump valve integrated unit, all ingredients pipe and control valve road are set in this subregion.
Refer to shown in Fig. 2 b-2c, it is the structural representation of body supports assembly of the present invention; The body supports assembly of the embodiment of the present invention is made up of steel plate splicing, is fixed by the mode of splicing or screw fastening; It comprises base assembly 212, top plate member 2112, connects and composes the support frame assembly 211 of casing side frame, buffering system mount pad 213 with base assembly 212.Wherein, described support frame assembly 211 comprises left side vertical plate assembly 2116, the middle riser assembly 2115 separating left and right two tanks, right side riser assembly 2117 and is arranged on right side benefit slab 2119, side stand rearways board component 2118, first cable permanent seat 2113 and the first cable permanent seat 2114 be arranged in top plate member 2112 of right side riser forward portion, and the web plate 2133 linked together by each assembly.
Wherein, described left side vertical plate assembly 2116, middle riser assembly 2115 surround the reaction warehouse accommodating cavity 200 holding reaction warehouse assembly; Top plate member arranges the first cable permanent seat 2114, and left side casing is divided into the reaction warehouse accommodating cavity 200 of front side and the circuit card accommodating cavity 20 of rear side by this steel plate; Middle riser assembly 2115 and right side riser assembly 2117 surround reagent chamber 2001.The middle steel plate of top plate member is provided with the first bending plate 2141 and the second bending plate 2142, both are one-body molded, be respectively steel plate both sides upwards bending formed part, between the first bending plate 2141 and the second bending plate 2142, form wire casing, pass through for wire.
Described buffering system mount pad 213 comprises and is separately positioned on platform support panel 2132 on left side vertical plate assembly 2116 and middle riser assembly 2115 and Reagent Tube supporting seat 2131, described platform support panel 2132 is arranged platform assembly and the reaction warehouse assembly on it.
Casing of the present invention adopts plate to connect, not only easy for installation, and the structural strength that each assembly links together is high, can support the weight of reaction warehouse assembly, camera and pump valve integrated unit, and can provide stable, reliable environment.
Refer to shown in Fig. 3, it is the one-piece construction schematic diagram of DNA sequencer reacting record sub-system of the present invention, reacting record sub-system in the embodiment of the present invention comprises reaction warehouse assembly 12, motion platform assembly 14, motion control unit 15, CCD camera 101 and platform supporting component 13, wherein
Described reaction warehouse assembly 12, arranges reaction chip assembly in it, under the effect of Controlling System, reaction solution enters in reaction chip and carries out chemical reaction, produces visible ray; Described motion platform assembly 14, CCD camera is fed into suitable picture-taking position by it, to take pictures collection to the visible ray information produced in reaction chip; Described platform assembly 13, its support described in reaction warehouse assembly 12, motion platform assembly 14; Described motion control unit, it controls in real time to the operation of CCD camera and reaction chip.
In embodiments of the present invention, described reaction warehouse assembly 12 can be installed, fix and reaction chip described in adjusting, ensure that reaction chip reacts in stable environment, and reaction chip can conveniently take out and lock.
Refer to shown in Fig. 4, described reaction warehouse assembly 12 comprises the door frame 121 of the vertical setting be arranged on described platform assembly 13, the top in the outside of door frame 121 is provided with turning reaction warehouse assembly 124, turnable basement 122 is connected with in the lower end of turning reaction warehouse assembly 124, turnable basement 122 is fixed on the middle part of door frame 121, turning reaction warehouse assembly 124 can rotate around described turnable basement 122, in order to take out or to put into reaction chip.
In embodiments of the present invention, described door frame 121 is fixedly connected with platform assembly 13, and is arranged on the front end of platform assembly 13, on rear side of door frame 121, be provided with CCD camera, door frame 121 is the structure that two sides have sidepiece baffle plate 1211, and it is one-body molded; Form curved cavity in the one side of door frame, turning reaction warehouse assembly 124 is installed in cavity.
In the present embodiment, described door frame 121 has enough hardness, when installing turning reaction warehouse assembly 124, can ensure that door frame 121 has minimum degree of crook; After installation reaction chip, described CCD camera contacts with door frame 121, takes pictures to reaction chip.
Also be provided with electronic lock assembly 125 in the left side of the upper end of described door frame 121, only when electronic lock assembly 125 is opened, turning reaction warehouse assembly 124 can be rotated, reaction chip is operated.In embodiments of the present invention, be provided with door frame 121, support reaction chip and dependency structure, substantially increase the stability of system.
Turning reaction warehouse assembly 124 comprises turning warehouse, is provided with back shroud 1242, in order to encapsulate Reagent Tube at the middle portion of described turning warehouse; Be provided with the first rotating part in bottom, it comprises the first container cavity 1243, and in order to hold turnable basement 122, the first container cavity 1243 is arranged on the mid-way of bottom; Be symmetrically arranged first lug 1244 in the first container cavity both sides, the centre of the first lug 1244 is provided with through hole 1246; In order to be connected with described turnable basement 122.Turnable basement rotates in the first container cavity 1243 relative to turning warehouse, has higher stability, and the transmission of guaranteed reagent,G.R. and data is unimpeded simultaneously.
Refer to shown in Fig. 4, turnable basement in the embodiment of the present invention 122 has the connection base of two the second lugs 1221, second lug 1221 is symmetricly set on a side 1223 of base, communicating pores 1224 is provided with in the middle of it, coaxially install with the through hole 1246 arranged in the middle of the first lug 1244 on described turning warehouse, further, the second lug 1221 is arranged on the inner side of the first lug 1244.
Shown in Fig. 2, in embodiments of the present invention, the both sides of described turnable basement 122 to be arranged in sidepiece baffle plate 1211 and to contact with sidepiece baffle plate 1211 and locate; The first described lug 1224 is after installing with the second lug 1221 is coaxial, the upper-end contact of end and turnable basement 122, and, the sidepiece of turning warehouse contacts with sidepiece baffle plate 1211 medial surface of door frame 121, ensures that turning warehouse and turnable basement 122 are just positioned at sidepiece baffle plate 1211.
Refer to shown in Fig. 5 a and 5b, in the inner side of the centre of described turning warehouse, reaction chip assembly is installed, accordingly, the first cavity 1785 holding reagent pipeline is provided with in the centre of turning warehouse, the bottom of the first cavity 1785 is provided with the first boss 1784, first boss 1784 is in order to carry the strut member of described reaction chip assembly, first boss 1784 is provided with four tail rod open holess 1786, tail rod open holes 1786 is through the two ends of turning warehouse, reaction chip assembly and strut member, reaction chip assembly is connected with turning warehouse, the second boss 1783, second boss 1784 is provided with in order to locate described strut member in the upper end of the first described boss 1784, the second described boss 1784 upper end is provided with locating surface 1782, locating surface 1782 is provided with locking screw nail 1787.Reaction chip assembly described in above-mentioned first cavity configuration location, and reaction chip assembly, when normally working, can not rotate, and is merely able to move along tail rod.
The upper surface of the first container cavity 1243 of described turning warehouse is provided with Reagent Tube open holes 1788 and electric wire open holes, pipe clamp 03 is installed in Reagent Tube open holes 1788, through having Reagent Tube 02 and electric wire 01 in pipe clamp 03.
The dead bolt open holes of the dead bolt 1252 in electronic lock assembly 125 is also provided with at the sidepiece of described turning warehouse; Under the effect of locking piece, turning warehouse and door frame 121 are fixed together.
The upper end in the outside of described turning warehouse is provided with pressing piece 1241, only described turning warehouse can be made to rotate by pressing piece 1241 pressing setting.In embodiments of the present invention, shown in Fig. 8, the structure that described pressing piece 1241 one end are provided with opening 1730, one end is provided with hook, described hook is connected with the upper end-stopping plate of door frame, when pressing piece 1241 is subject to downward reactive force, hook and door frame baffle plate depart from, and turning reaction warehouse body can rotate relative to door frame.
In embodiments of the present invention, described pressing piece 1241 is arranged in a positioning block 1762, the lower end of preset pieces 1762 is provided with spring 1761, spring is arranged in spring support 1763, and spring support 1763 screw thread is arranged in the spring base hole 1781 of intermediate throughholes 1785 upper surface of turning reaction warehouse.
Shown in Fig. 5 b and 6a, preset pieces 1762 is arranged in the preset pieces installation cavity 1791 of turning reaction warehouse upper end, is provided with positioning step 1790 in cavity.Preset pieces 1762 in the embodiment of the present invention comprises through hole 1794 and lower through-hole 1797, both one of by described pressing piece 1241; Locating and machining hole 1796 and open holes 1795 are set in the side of preset pieces.The lower end of the through hole installing pressing piece 1241 is provided with spring eye, and spring is contacted with the bottom of pressing piece 1241 by this hole.
The embodiment of the present invention arranges electronic lock assembly and pressing piece, carries out twice locking to turning reaction warehouse assembly and door frame, adopts mode that is automatically controlled and physical construction respectively, ensures that reaction chip runs in security context, avoids mishandle.
Shown in Fig. 6 b and 6c, electronic lock assembly 125 of the present invention comprises electronics lock and inductive switch 1251, described inductive switch is arranged on electronics lock, electronics lock is arranged on the sidepiece of turning warehouse, in embodiments of the present invention, electronics lock comprises sidepiece mounting plate 1843, and sidepiece mounting plate 1843 offers open holes 1844; Electronic lock is done to be fixed on turning warehouse by open holes 1844 and mounting screw.Be provided with the first symmetrical framework 1841 and the second framework 1842 in described sidepiece mounting plate 1843 side, leave the space of installing inductive switch 1251 in the centre of the first framework and the second framework.
In embodiments of the present invention, described inductive switch 1251 comprises the framework 1255 being arranged on outside, and a side of framework 1255 is provided with connecting thread hole 1256, and itself and described electronics lock are fixed by coupling screw 1254; Described coupling screw 1254 by the lug 1258 on side and the first framework 1841, second framework 1842, in order to increase the strength of joint of electronics lock.Described inductive switch 1251 is also connected with control circuit 1252, in order to control inductive switch 1251.
Refer to shown in Fig. 6 d, described electronic lock assembly 125 is arranged on the sidepiece of turning warehouse and door frame 121, enter after dead bolt 1253 stretches out be arranged on turning warehouse sidepiece dead bolt hole 1247 in, realize locking; When under the effect of dead bolt 1253 in electricity irritation, when retracting in dead bolt hole 1247, described turning warehouse can be rotated.
The use of door frame, not only can make the Stability Analysis of Structures of reaction chip assembly, and, also make turning warehouse and turnable basement compact construction, convenient operation; The location of CCD camera and reaction chip is more accurate.
The first open holes 1210 is provided with, in order to be connected and fixed with motion platform assembly 14 in the bottom of door frame 121; The second open holes be connected with turnable basement sidepiece is also provided with in the middle part of door frame.
Shown in Fig. 7, in embodiments of the present invention, in order to save the stable of structure space and guaranteed reagent,G.R. transmission, Reagent Tube first fixation kit is provided with in the through hole 1246 arranged in the middle of the first described lug 1244 and the communicating pores of the second lug 1221, it comprises the pipe joint 1265 be arranged in the lug of left side, first packing ring 1264, first turning axle 1261, and the Reagent Tube joint 1266 be arranged in the lug of right side, second packing ring 1267, second turning axle 1269, wherein, the first described packing ring 1264 and the second packing ring 1267 are arranged between turning warehouse and turnable basement, in the present embodiment, the diameter of the through hole 1246 arranged in the middle of the first described lug 1244 is less than the diameter of the communicating pores of the second lug 1221, the location rib to packing ring location is formed at both medial ends, after installing two packing rings, inside diameter of gasket is just identical with the diameter of the communicating pores of the second lug 1221.Location rib and packing ring are set in embodiments of the present invention, ensure gapless between turning warehouse and turnable basement, both good stabilities after combining, can not rock because there is gap, and, packing ring adds both contact area, and stress is between the two reduced, extension device work-ing life.
The structure that the inner side of through hole 1246 arranged in the middle of the first lug 1244 and the inner side of packing ring are provided with the first turning axle 1261 and the second turning axle 1269, two turning axles is identical, is the quill shaft with positioning convex ring; In one end of turning axle, positioning convex ring is set, be provided with connecting thread hole, in order to be connected and fixed with the lug connection portion 1263 of described turning warehouse, accordingly, be provided with the positioning recess of locating described positioning convex ring in the end of described through hole 1246.Be recess in the centre at the both ends of turning axle 1269, inner side recess is in order to install locate with described pipe joint 1265, Reagent Tube joint 1266.In pipe joint 1265, Reagent Tube joint 1266, be separately installed with electric wire and Reagent Tube 02, it leads in reaction chip assembly respectively.Owing to arranging the assembly that can mutually overturn in the embodiment of the present invention, when overturning, for avoiding impacting pipe fitting, in through-holes Reagent Tube being installed, substantially increasing the safety performance of Reagent Tube.
Refer to shown in Fig. 8, in embodiments of the present invention, described reaction chip assembly is arranged on the inner side of described turning warehouse.Be provided with the observation section 1724 contacted with CCD camera in the inner side of reaction chip assembly, be provided with transparent baffle plate, to take pictures to reaction process.
Refer to shown in Fig. 9 a, the shading positioning component 128 that reaction chip assembly of the present invention comprises reaction chip mount pad 127, reaction warehouse base assembly 126 and reaction warehouse base assembly 126 and reaction chip mount pad 127 locked, opens; In order to take out reaction chip easily and close, reaction chip mount pad 127 can rotate relatively with reaction warehouse base assembly 126.
Refer to shown in Fig. 9 b, it is the structural representation of reaction chip mount pad of the present invention, is provided with reaction chip at the middle portion of reaction chip mount pad 127, and reaction chip is provided with cover plate 1277; In the both sides of reaction chip, chute pressing plate 1279 is installed, in order to be fixed reaction chip.A side portion of reaction chip mount pad 127 is provided with mount pad supporting component, its in order to take out or chip placement time, the reaction chip mount pad described in support; It comprises torsional-spring supported portion 1273, be arranged on mount pad, in order to support described torsion spring, described torsional-spring supported portion 1273 is one with the recess of protruding end 1274, when the reaction chip mount pad 127 described in rotating, protruding end 1274 contacts with 1261 at first, and in the present embodiment, described protruding end 1274 is arranged on the middle part at the edge of recess and chip installation position; Adopt the structure of mount pad itself to support reaction chip mount pad, better reliability, ensure that chip can take out safely and place.
Described mount pad supporting component also comprises torsion spring 1272, be connected with torsion spring 1272 and be arranged on the tension axle 1271 at torsion spring two ends, described tension axle 1271 is arranged on lug boss 1275, when starting reaction chip mount pad, described torsion spring 1272 supports mount pad, and the final location relying on protruding end 1274, realize supporting.
The first rotary shaft hole 1276 is also provided with, in order to be connected with reaction warehouse base 1261 in the end of described lug boss 1275.Locating slot 1278 is also provided with at the sidepiece of reaction chip mount pad.
Refer to shown in Fig. 9 c, it is the structural representation of the medial surface of reaction warehouse base of the present invention, reaction tank body 1266 is provided with in the medial surface of reaction warehouse, at reaction tank body 1266 middle portion, reaction tank 1267 is set, the surrounding 1706 of reaction tank 1267 is provided with sealing-ring, prevents reaction reagent from leaking.One end of reaction warehouse base 1261 is provided with raised line 1705, in order to install after cover plate locate.The other end is provided with the torsional-spring supported structure corresponding with reaction chip mount pad, and it comprises symmetrically arranged two protruding ends 1268, is provided with the second rotary shaft hole 1269 thereon, and it is coaxial with the first rotary shaft hole 1276, and turning axle is successively through holes; Also being provided with the region 1260 for torsion spring activity and locating area 1707 in this end, being provided with in this region in order to the threaded hole 1712 by installing reaction warehouse cover plate.
Refer to shown in Fig. 9 d, it is the structural representation of the outer side of reaction warehouse base of the present invention, the middle portion of the outer side of reaction warehouse base is provided with temperature control assembly, the middle portion of reaction warehouse base is provided with temperature sensor mounting groove 1262, the bottom of mounting groove 1262 is provided with nichrome wire mounting groove 1704, places nichrome wire and temperature sensor respectively.In mounting groove, be also provided with a positioning boss 1703, locate when it is in order to install with temperature control assembly cover plate 1263.Temperature control assembly cover plate 1263 is provided with the positioning recess 1702 corresponding with positioning boss 1703.
The surrounding of temperature sensor mounting groove 1262 is provided with four tail rod open holess 1742, upper and lower sides is provided with self-adaptation spring mounting hole 1741; Cover plate 1263 is also provided with guiding rod aperture 1701.
Reaction warehouse base is also provided with shading positioning component mounting groove 1265, in order to install described shading positioning component 128, is provided with installation base 1744 within it.
Shown in Figure 10 a-10b, the middle part of reaction warehouse base is provided with electrical heating wire 1754, it is arranged in the nichrome wire mounting groove 1704 of base; In the temperature sensor mounting groove 1262 on electrical heating wire top, be provided with temperature sensor 1720 and control unit, in order to detection reaction pond temperature and according to setting threshold value regulate; Also water at temperature sensor 1720 and control unit 1753 and have sealing member 1263, it is encapsulating resin.Be respectively arranged with Reagent Tube at the two ends of reaction tank and threaded hole 1721 be installed, in order to be connected with Reagent Tube; The end installing threaded hole 1721 at Reagent Tube is provided with fluid inlet 1723 and liquid outlet 1713, and liquid inlet and outlet communicates with the two ends of reaction tank, in order to cycle detection reagent.
The avris of the interior grooves of reaction tank is provided with sealing-ring 1713.Be provided with reaction chip 04 in the centre of reaction chip mount pad, reagent flows through reaction chip and reacts.
In embodiments of the present invention, Reagent Tube 02 is by the Reagent Tube open holes 1788 of turning warehouse, and the centre being arranged on turning warehouse is provided with the first cavity 1785 holding reagent pipeline, final ligation pond body, reagent cartridge is through the sealing of various cavity, and stability is high, not fragile.
Back shroud 1242 is fixed with at the rear side of reaction warehouse, the centre of back shroud 1242 is provided with self-adaptative adjustment part 1751, refer to shown in Figure 12 a-12c, in the middle of the inner side of back shroud 1242, be provided with two rows raised line 1809 is installed, the centre of installing raised line 1809 is provided with the first installation base 1812 and the second installation base 1811, installation base offers threaded hole 1802, and self-adaptative adjustment part 1751 is arranged in this threaded hole 1802.
In embodiments of the present invention, described self-adaptative adjustment part 1751 is thread end with the coupling end of back shroud, is the polished rod 1805 being provided with spring 1806, is provided with nut 1804 between the ends and positions with the coupling end of reaction warehouse assembly, after installation is complete, nut 1804 is close to installation base.
Be passage 1803 between described two raised lines 1809, Reagent Tube can be made to pass through; Back shroud is also provided with the reinforcement gusset 1812 vertical with raised line 1809; At upper surface 1810 bit plane of raised line 1809 with boss; Below, the centre of the peripheral framework 1807 of plate and corner are provided with fastening screw nail 1801, in order to be connected and fixed with described turning reaction warehouse.
Above-mentioned back shroud assembly, not only can carry out face seal to reaction warehouse assembly, and can also install fixing self-adaptative adjustment part, structure is simple.
In embodiments of the present invention, between turning reaction warehouse and reaction warehouse base 126, be provided with self-adaptative adjustment part 1751, the end spring of self-adaptative adjustment part 1751 and the end contact of reaction warehouse base; When using CCD camera feeding, when camera and reaction chip component touch, when adjusting camera position, movable mode can be adopted to adjust, now, self-adaptative adjustment part can change the position of reaction chip assembly according to the change in location of camera, ensure to have higher accuracy of detection, meanwhile, avoid causing damage to system.
Refer to shown in Figure 13 a-13b, the platform assembly of the embodiment of the present invention comprises vibration damper plate 1497 and is arranged on the first damping wing plate 1492 and the second damping wing plate 1491 of vibration damper plate 1497 both sides, is respectively arranged with dampening assembly 1493 in the lower end of two damping wing plates.After installation is complete, platform assembly is indentations, and reaction warehouse assembly 12 is arranged on vibration damper plate 1497, and two ends contact with two vibration damper plates respectively.
Damping wing plate comprises back up pad 1497, and it has certain height, the reaction warehouse assembly described in support; The centre of the integrated upper head plate 1495 of back up pad 1497 is provided with the mounting groove 1492 of motion control unit 15, is provided with the circuit card of motion control unit 15 and various circuit in it; Be stiffening plate 1496 in the lower end of mounting groove 1492, in order to offer mounting groove 1492.The platform assembly of the embodiment of the present invention can either be symmetrical arranged the stability of guarantee system, can arrange control unit again, saves space, compact construction.
Platform assembly 13 is also provided with motion platform assembly 14 and CCD camera 101, refer to shown in Figure 16, described motion platform assembly 14 comprises motor 136, the lead screw guide rails assembly be connected with motor output shaft, the supporting seat supporting lead screw assembly and detection components, camera support assembly.
Refer to shown in Figure 14, the output shaft of motor 136 is connected by shaft coupling 1377 with leading screw 137, and motor and leading screw junction are provided with supporting seat 1361, is provided with bearing 1371 and axial positioning sleeve 1376 in the end of leading screw.Leading screw is connected with nut 1374, and nut is arranged on nut mount pad 1375; The upper end of nut mount pad 1375 is connected with web plate 1012, and the upper end of web plate 1012 is connected with camera mount pad 1011; At bearing end, bearing friction top 1372 is set.
Described nut mount pad 1375 is arranged on the slide block 1012 that contacts with guide rail 132, and on slide block, drive camera to move.
The agent delivery system of the embodiment of the present invention comprises control unit, damping fluid case loading/unloading platform assembly, liquid case lifting gantry assembly, pump valve integrated pipe assembly, wherein, described damping fluid case loading/unloading platform can load and unload all ingredients, and be transported to the position of specifying up and down through liquid case lifting gantry assembly, for described pump valve integrated pipe Component driver, the Reagent Tube that described pump valve integrated pipe component selection needs, through the driving of hydro-pump and the open and close controlling of hydraulicvalve, the reagent delivery of specifying is reacted to reaction warehouse assembly; The action of each element above-mentioned controls by control unit is unified.
Refer to shown in Figure 16 a-16b, the liquid case lifting gantry assembly of the embodiment of the present invention is the mechanism that pumps that motor leading screw drives, and it is arranged in the right side casing of sequenator.It comprises drive-motor 401, be connected with drive-motor and the supporting seat 402 of support drive motor, the lead screw assembly be connected with drive-motor, be arranged on the reaction liquid case elevating bracket 408 of sidepiece, wherein, damping fluid case loading/unloading platform assembly is equiped with in described reaction liquid case elevating bracket 408, reaction is arranged on the mounting plate 404 that is connected with reaction liquid case elevating bracket with liquid case elevating bracket 408, under the driving of screw mechanism, carrying reaction liquid case elevating bracket Reagent Tube group moves up and down.
Reaction comprises the intermediate plate 4081 be connected with mounting plate with liquid case elevating bracket 408, be arranged on the supporting plate 4082 of intermediate plate both sides, supporting plate 4082 is provided with horizontal ends, in order to place damping fluid case loading/unloading platform assembly.
The output shaft of described drive-motor 401 is connected with leading screw 403, and leading screw is arranged in base 405, and base 405 is connected by supporting seat 402 with the end of drive-motor 401; The sidepiece of base 405 is provided with stroke detection switch 406.The output shaft of described drive-motor is connected with the coupling end 4034 of leading screw by shaft coupling 4035; Leading screw is connected with nut 4031, nut is fixed on nut seat 4032; Leading screw lower end is by bearings, and bearing end is provided with bearing (ball) cover 4011.
The liquid case lifting gantry assembly of the embodiment of the present invention by guide rail movement by reagent delivery to specified location, it is convenient, precisely to control; Pump valve integrated pipe assembly and damping fluid case loading/unloading platform assembly transport by liquid case lifting gantry up and down, complete reagent conveying, reaction process.
Refer to Figure 17 a-18b to show, reagent is sucked by the syringe needle suction pipe 507 of downside by the pump valve integrated pipe assembly of the embodiment of the present invention from the Reagent Tube damping fluid case loading/unloading platform assembly, and pass through the selection of fluid path control unit 502, drived control valve module 509 and hydro-pump 506 are selected, reagent is entered in reagent cartridge, carry out sequencing reaction.
Described pump valve integrated pipe assembly comprises pump valve integrated base plate 508, retaining plate, base plate that pump valve integrated base plate 508 comprises peristaltic pump mounting plate 5011, is connected with peristaltic pump mounting plate 5011, be arranged on the sipper pore group on base plate and the control valve unit installing board in the middle of base plate; Peristaltic pump mounting plate 5011 is provided with peristaltic pump open holes, it is arranged two peristaltic pumps 505; On base plate, also interval arranges multiple reagent pipe clamp and wire clamp 5015.Accordingly, control valve assembly 509 is arranged on the middle portion of pump valve integrated base plate 508, and hydro-pump 506 is arranged on its sidepiece, arranges fluid path control unit 502 in the other side, and fluid path control unit 502 is isolated with fluid path part by a bending plate 503.
In embodiments of the present invention, in order to carry out sequencing reaction to plurality of reagents, the sipper pore group on base plate has been arranged side by side multiple hole, and syringe needle suction pipe 507 is through sipper pore, and the other end connects Reagent Tube and is connected with the fluid path port of corresponding control valve; Sipper pore arranges pipe clamp 5013, prevents syringe needle suction pipe from tilting.
The embodiment of the present invention, adopt pump valve integrated pipe assembly, can select plurality of reagents simultaneously, and carry out sequencing reaction according to the order pre-set, testing efficiency is high.
Refer to shown in Figure 18 a-18b, damping fluid case loading/unloading platform assembly is the structure of drawing and pulling type, and after platform sets reagent, whole platform assembly is arranged in the casing of right side, and when needs handling reagent, pulled out by platform, after placing, pushed home is put again.
It comprises carrier fluid platform 310, and in the present embodiment, it is one with the arm-tie of handle 3101, and handle is fixed on handle plate; The both sides of carrier fluid platform 310 are provided with web plate 311, and the horizontal part of web plate 311 is connected with the supporting plate of liquid case elevating bracket 408 with reaction; Under the effect of reaction with liquid case elevating bracket, carrier fluid platform 310 moves up to syringe needle suction pipe 507 place on the downside of pump valve integrated pipe, through the control of control unit, selectively draws reagent.
Between described carrier fluid platform 310 and the web plate 311 of both sides, arrange slide track component 312, it makes carrier fluid platform move along web plate.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and this embodiment is also not used to limit the scope of the claims of the present invention, and the equivalence that all the present invention of disengaging do is implemented or changed, and all should be contained in the scope of technical solution of the present invention.
Claims (7)
1. one kind has the dual box DNA sequencer of screening of door frame, it is characterized in that, it comprises reacting record sub-system, agent delivery system and Controlling System, and carry the sequenator casing of above-mentioned each system, wherein, described reacting record sub-system and agent delivery system are separately positioned in the interval, the left and right sides of sequenator casing; The rear side of the reacting record sub-system of described set-up of control system on the left of casing in interval, control reaction sub-system and agent delivery system according to predetermined sequential action;
Described reacting record sub-system comprises reaction warehouse assembly, motion platform assembly, motion control unit, CCD camera and platform supporting component, described reaction warehouse assembly, reaction chip assembly is set in it, under the effect of Controlling System, reaction solution enters in reaction chip and carries out chemical reaction, produces visible ray; Described motion platform assembly, CCD camera is fed into suitable picture-taking position by it, to take pictures collection to the visible ray information produced in reaction chip to make CCD camera; Described platform assembly, its support described in reaction warehouse assembly, motion platform assembly;
Described reaction warehouse assembly comprises the door frame of the vertical setting be arranged on described platform assembly, the lower end of described door frame is fixedly connected with platform assembly, and be arranged on the front end of platform assembly, on rear side of door frame, be provided with described CCD camera;
Install by reaction chip in the middle of described reaction warehouse assembly;
Form curved cavity in the one side of door frame, turning reaction warehouse assembly is installed in cavity, and the turnable basement be rotatably connected with turning reaction warehouse assembly.
2. the dual box DNA sequencer of screening with door frame according to claim 1, it is characterized in that, described door frame is the structure that two sides have sidepiece baffle plate, and it is one-body molded;
Described turnable basement is fixed on the middle part of door frame.
3. the dual box DNA sequencer of screening with door frame according to claim 2, it is characterized in that, described sequenator casing comprises body supports assembly, is arranged on the interior lining panel assembly inside body supports assembly;
Casing is divided into left and right two portions, is provided with reacting record sub-system in the casing of left side; In right case, be provided with agent delivery system, comprise pump valve pipe integrated unit, liquid case lifting gantry and indicating meter, the rear side of casing is provided with control unit.
4. the dual box DNA sequencer of screening with door frame according to claim 3, it is characterized in that, described body supports assembly is made up of steel plate splicing, is fixed by the mode of splicing or screw fastening; It comprises base assembly, top plate member, connects and composes the support frame assembly of casing side frame with base assembly, buffering system mount pad;
Wherein, described support frame assembly comprises left side vertical plate assembly, middle the riser assembly separating left and right two tanks, right side riser assembly and is arranged on the right side benefit slab of right side riser forward portion, side stand rearways board component, is arranged on the first cable permanent seat in top plate member and the first cable permanent seat, and by web plate that each assembly links together.
5. the dual box DNA sequencer of screening with door frame according to claim 3, it is characterized in that, electronic lock assembly is also provided with in the left side of the upper end of described door frame, described electronic lock assembly comprises electronics lock and inductive switch, described inductive switch is arranged on electronics lock, and electronics lock is arranged on the sidepiece of turning warehouse;
Electronics lock comprises sidepiece mounting plate, and sidepiece mounting plate offers open holes;
Electronic lock is done to be fixed on turning warehouse by open holes and mounting screw;
Be provided with the first symmetrical framework and the second framework in described sidepiece mounting plate side, leave the space of installing inductive switch in the centre of the first framework and the second framework.
6. the dual box DNA sequencer of screening with door frame according to claim 4, it is characterized in that, described turning reaction warehouse assembly comprises turning warehouse, in the inner side of the centre of described turning warehouse, reaction chip assembly is installed, accordingly, be provided with the first cavity holding reagent pipeline in the centre of turning warehouse, the bottom of the first cavity is provided with the first boss, and the first boss is in order to carry the strut member of described reaction chip assembly.
7. the dual box DNA sequencer of screening with door frame according to claim 4, it is characterized in that, described agent delivery system comprises control unit, damping fluid case loading/unloading platform assembly, liquid case lifting gantry assembly, pump valve integrated pipe assembly, wherein,
Described damping fluid case loading/unloading platform can load and unload all ingredients, and be transported to the position of specifying up and down through liquid case lifting gantry assembly, for described pump valve integrated pipe Component driver, the Reagent Tube that described pump valve integrated pipe component selection needs, through the driving of hydro-pump and the open and close controlling of hydraulicvalve, the reagent delivery of specifying is reacted to reaction warehouse assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510726802.5A CN105273998B (en) | 2015-10-30 | 2015-10-30 | Double-shielding box-type DNA sequencer with portal |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510726802.5A CN105273998B (en) | 2015-10-30 | 2015-10-30 | Double-shielding box-type DNA sequencer with portal |
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| CN105273998A true CN105273998A (en) | 2016-01-27 |
| CN105273998B CN105273998B (en) | 2017-04-12 |
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| CN201510726802.5A Expired - Fee Related CN105273998B (en) | 2015-10-30 | 2015-10-30 | Double-shielding box-type DNA sequencer with portal |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105219640A (en) * | 2015-11-02 | 2016-01-06 | 北京中科紫鑫科技有限责任公司 | A kind of box DNA sequencer with frame shock-damping structure |
| CN105296346A (en) * | 2015-11-02 | 2016-02-03 | 北京中科紫鑫科技有限责任公司 | Box-type DNA sequencer of dual shockproof framework with lining plates |
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| CN102703314A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Control system for DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703302A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102703311A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102766574A (en) * | 2012-05-24 | 2012-11-07 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102703314A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Control system for DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703302A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
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| CN105219640A (en) * | 2015-11-02 | 2016-01-06 | 北京中科紫鑫科技有限责任公司 | A kind of box DNA sequencer with frame shock-damping structure |
| CN105296346A (en) * | 2015-11-02 | 2016-02-03 | 北京中科紫鑫科技有限责任公司 | Box-type DNA sequencer of dual shockproof framework with lining plates |
| CN105219640B (en) * | 2015-11-02 | 2017-09-08 | 北京中科紫鑫科技有限责任公司 | A kind of box DNA sequencer with frame shock-damping structure |
| CN105296346B (en) * | 2015-11-02 | 2017-09-12 | 北京中科紫鑫科技有限责任公司 | A kind of box DNA sequencer of the dual shockproof framework with liner plate |
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| CN105273998B (en) | 2017-04-12 |
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