CN105273096A - Simple technology for combined extraction of pleurotus geesteranus sporophore protein and polysaccharide - Google Patents
Simple technology for combined extraction of pleurotus geesteranus sporophore protein and polysaccharide Download PDFInfo
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Abstract
The present invention provides a simple technology for combined extraction of pleurotus geesteranus sporophore protein and polysaccharide, and the method is achieved by the following technical solution: pleurotus geesteranus sporophore is cut into small pieces, the pleurotus geesteranus sporophore small pieces are taken and frozen to be solid at -80 DEG C, and then freeze-dried at -40 DEG C, the freeze-dried material is pulverized and sieved, the resultant pleurotus geesteranus sporophore powder is added with water in a certain proportion, sodium hydroxide is used to adjust pH, the pleurotus geesteranus sporophore powder is extracted at 55-60 DEG C, after the alkaline extract is cooled to room temperature, and centrifuged, the supernatant is adjusted to pH of 4.5 with hydrochloric acid, then the precipitate, namely a pleurotus geesteranus protein extract, is obtained after centrifugation; the supernatant obtained after centrifugation is concentrated to an appropriate volume, the concentrated solution is precipitated with 95% ethanol being 3 times of the volume of the concentrated solution, and the centrifuged precipitate is freeze-dried to obtain a pleurotus geesteranus polysaccharide extract.
Description
Technical field
The present invention relates to a kind of technology extracting Pleurotus geesteranus activeconstituents, specifically a kind of technology of combined extracting Pleurotus geesteranus sporophore egg bletilla polysaccharide, belong to biochemical engineering technical field.
Background technology
Pleurotus geesteranus is under the jurisdiction of Basidiomycetes, Agaricales, Pleurotaceae, pleurotus, it is tender and crisp, nutritious, the colory edible mushrooms of a kind of quality, be rich in polysaccharide and vitamins and other nutritious components, wherein Pleurotus geesteranus polysaccharide is because having excellent anti-oxidant, anti-tumor activity and receiving much concern.But Pleurotus geesteranus is rich in proteins also, be a kind of important nutritive substance, to human body, there is good health value.At present, extraction process both at home and abroad about Pleurotus geesteranus polysaccharide has many reports, but existing technical matters is when extracting Pleurotus geesteranus activeconstituents, only considers this kind of composition of polysaccharide, and the higher protein of content is often dropped, this is a kind of waste greatly concerning the Pleurotus geesteranus resource of preciousness; In addition, both at home and abroad about the extraction research also rarely seen report of Pleurotus geesteranus albumen.Therefore, adopt a kind of easy method, extract Pleurotus geesteranus albumen and the main activeconstituents of polysaccharide these two kinds simultaneously, for aspects such as efficiency utilization Pleurotus geesteranus resource and exploitation health food, there is important using value.Beneficial effect of the present invention shows following two aspects: (1) the present invention can be exploitation Pleurotus geesteranus health food and provides Technical Reference; (2) the present invention can combined extracting two kinds of activeconstituentss, efficiency utilization Pleurotus geesteranus resource.
Summary of the invention
Patent object of the present invention is for solving the problem and providing the easy technique of a kind of combined extracting Pleurotus geesteranus albumen and polysaccharide, can efficiency utilization Pleurotus geesteranus resource, improves its using value and scope.
Patent of the present invention is achieved through the following technical solutions: Pleurotus geesteranus sporophore is cut into small pieces-frozen drying-pulverize and sieve-with alkali lye extract-centrifugal-supernatant liquor acid heavy-centrifugal albumen-supernatant concentration-alcohol settling-centrifugal polysaccharide.
Described combined extracting Pleurotus geesteranus polysaccharide and the technical matters of albumen, comprise following concrete steps:
(1) choose Pleurotus geesteranus sporophore, clean and be filtered dry surface-moisture, be cut into fritter in small, broken bits by knife;
(2) get sporophore fritter and freeze reality in-80 DEG C ,-40 DEG C of lyophilizes more than 24 hours;
(3) pulverized by freeze-dried material pulverizer, then 80 ~ 100 orders sieve to obtain Pleurotus geesteranus sporophore powder;
(4) sporophore powder adds water by the material-water ratio of 1:60 ~ 65, regulates pH to 11.0 with sodium hydroxide, extracts 2 ~ 2.5 hours in 55 ~ 60 DEG C;
(5), after above-mentioned alkali extracting solution being cooled to room temperature, 4800 revs/min are centrifugal 10 minutes, and supernatant liquor hydrochloric acid is adjusted to pH4.5, then 4800 revs/min centrifugal 15 minutes, gained precipitation be Pleurotus geesteranus protein extract;
(6) by the above-mentioned centrifugal supernatant concentration obtained to proper volume, with 95% alcohol settling more than 24 hours of 3 times of volumes, then 4800 revs/min centrifugal 15 minutes, gained precipitation within 12 ~ 16 hours, be Pleurotus geesteranus polyoses extract through lyophilize.
Adopt the effect of said extracted scheme: Pleurotus geesteranus albumen average recoveries is 16.27%, and Pleurotus geesteranus polysaccharide average recoveries is 5.66%.
Embodiment
In following embodiment, method therefor is ordinary method if no special instructions.
Embodiment 1: accurately take 60 grams of Pleurotus geesteranus sporophores, cleans and is filtered dry surface-moisture, and shred;-80 DEG C freeze in-40 DEG C of lyophilizes 24 hours in fact, and after pulverizing with pulverizer, 80 orders sieve and obtain powder.By material-water ratio 1:60 by Pleurotus geesteranus sporophore powder and water mixing, by alkali adjust ph 11.0, then extract 2.5 hours in 55 DEG C; After being cooled to room temperature, 4800 revs/min centrifugal 10 minutes, and supernatant liquor hydrochloric acid is adjusted to pH4.5, and 4800 revs/min centrifugal 15 minutes, obtains Pleurotus geesteranus albumen.Again by the above-mentioned centrifugal supernatant concentration obtained to 150ml, with 95% alcohol settling 26 hours of 3 times of volumes, then centrifugal 15 minutes in 4800 revs/min, gained precipitation, through-40 DEG C of lyophilizes 12 hours, obtains Pleurotus geesteranus polysaccharide.The protein extracting ratio according to said method obtained is 16.03%, and polysaccharide extract rate is 5.83%.
Embodiment 2: accurately take 80 grams of Pleurotus geesteranus sporophores, cleans and is filtered dry surface-moisture, and shred;-80 DEG C freeze in-40 DEG C of lyophilizes 26 hours in fact, and after pulverizing with pulverizer, 90 orders sieve and obtain powder.By material-water ratio 1:65 by Pleurotus geesteranus sporophore powder and water mixing, regulate pH to 11.0 with alkali, then extract 2 hours in 55 DEG C; After being cooled to room temperature, 4800 revs/min centrifugal 10 minutes, and supernatant liquor hydrochloric acid is adjusted to pH4.5, and 4800 revs/min centrifugal 15 minutes, obtains Pleurotus geesteranus albumen.Again by the above-mentioned centrifugal supernatant concentration obtained to 200ml, with 95% alcohol settling 24 hours of 3 times of volumes, then centrifugal 15 minutes in 4800 revs/min, gained precipitation, through-40 DEG C of lyophilizes 14 hours, obtains Pleurotus geesteranus polysaccharide.The Pleurotus geesteranus protein extracting ratio according to said method obtained is 16.67%, and Pleurotus geesteranus polysaccharide extract rate is 5.54%.
Embodiment 3: accurately take 100 grams of Pleurotus geesteranus sporophores, cleans and is filtered dry surface-moisture, and shred;-80 DEG C freeze in-40 DEG C of lyophilizes 24 hours in fact, and after pulverizing with pulverizer, 100 orders sieve and obtain powder.By material-water ratio 1:65 by Pleurotus geesteranus sporophore powder and water mixing, regulate pH to 11.0 with alkali, then extract 2.5 hours in 60 DEG C; After being cooled to room temperature, 4800 revs/min centrifugal 10 minutes, and supernatant liquor hydrochloric acid is adjusted to pH4.5, and 4800 revs/min centrifugal 15 minutes, obtains Pleurotus geesteranus albumen.Again by the above-mentioned centrifugal supernatant concentration obtained to 250ml, with 95% alcohol settling 24 hours of 3 times of volumes, then centrifugal 15 minutes in 4800 revs/min, gained precipitation, through-40 DEG C of lyophilizes 16 hours, obtains Pleurotus geesteranus polysaccharide.The Pleurotus geesteranus protein extracting ratio according to said method obtained is 16.11%, and Pleurotus geesteranus polysaccharide extract rate is 5.61%.
Claims (5)
1. an easy technique for combined extracting Pleurotus geesteranus egg bletilla polysaccharide, is characterized in that: Pleurotus geesteranus sporophore is cut into small pieces-frozen drying-grinding and sieving-with alkali lye extract-centrifugal-supernatant liquor acid heavy-centrifugal Pleurotus geesteranus albumen-supernatant liquor alcohol settling-centrifugal Pleurotus geesteranus polysaccharide.
2., according to described in claim 1, first-80 DEG C of sporophore fritter freezes again in-40 DEG C of lyophilizes more than 24 hours in fact, and dried raw material after crushed 80 ~ 100 orders sieves.
3. according to described in claim 1 and claim 2, sporophore powder and water mix in the ratio of 1:60 ~ 65, regulate pH to 11.0 with sodium hydroxide, extract 2 ~ 2.5 hours in 55 ~ 60 DEG C.
4., according to described in claim 1 and claim 3, after above-mentioned alkali extracting solution is cooled to room temperature, 4800 revs/min centrifugal 10 minutes, supernatant liquor hydrochloric acid is adjusted to pH4.5, then centrifugal 15 minutes in 4800 revs/min, gained precipitation is Pleurotus geesteranus albumen, retains supernatant liquor.
5. according to described in claim 1 and claim 4, supernatant concentration to 8 ~ 10 times of volumes of dried powder weight, then uses 3 times to 95% alcohol settling more than 24 hours of concentrated solution volume, then centrifugal 15 minutes in 4800 revs/min,-40 DEG C of lyophilizes more than 12 hours, obtain Pleurotus geesteranus polysaccharide.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962590A (en) * | 2017-04-19 | 2017-07-21 | 河北师范大学 | A kind of method for preparing edible fungus protein powder |
| CN109043495A (en) * | 2018-07-25 | 2018-12-21 | 辽宁省农业科学院 | A kind of preparation method of edible fungi root extract |
| CN111777690A (en) * | 2020-07-27 | 2020-10-16 | 西华师范大学 | Pleurotus citrinopileatus polysaccharide and preparation method and application thereof |
| CN112156106A (en) * | 2019-11-11 | 2021-01-01 | 湖南万臻生物科技有限公司 | Medicine for treating cervical cancer and preparation thereof |
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| JPS5156804A (en) * | 1974-11-13 | 1976-05-18 | Nippon Kokan Kk | Fukusushuruino nenryogasuno kongokyokyuhoho |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962590A (en) * | 2017-04-19 | 2017-07-21 | 河北师范大学 | A kind of method for preparing edible fungus protein powder |
| CN109043495A (en) * | 2018-07-25 | 2018-12-21 | 辽宁省农业科学院 | A kind of preparation method of edible fungi root extract |
| CN112156106A (en) * | 2019-11-11 | 2021-01-01 | 湖南万臻生物科技有限公司 | Medicine for treating cervical cancer and preparation thereof |
| CN112156106B (en) * | 2019-11-11 | 2023-09-26 | 湖南万臻生物科技有限公司 | Medicine for treating cervical cancer and preparation thereof |
| CN111777690A (en) * | 2020-07-27 | 2020-10-16 | 西华师范大学 | Pleurotus citrinopileatus polysaccharide and preparation method and application thereof |
| CN111777690B (en) * | 2020-07-27 | 2022-02-08 | 西华师范大学 | Pleurotus citrinopileatus polysaccharide and preparation method and application thereof |
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