CN105267380B - A kind of preparation method of Chinese medicine composition that preventing and treating bacterial enteritis of aquatic livestock - Google Patents
A kind of preparation method of Chinese medicine composition that preventing and treating bacterial enteritis of aquatic livestock Download PDFInfo
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Abstract
A kind of preparation method of Chinese medicine composition that preventing and treating bacterial enteritis of aquatic livestock, specific steps are as follows: (1) pulverize purslane, the Chinese bulbul and Cortex Phellodendri, heating water mentions, and filters out solution and is concentrated；(2) chitosan glacial acetic acid solution is prepared in heating；(3) Chinese medical concrete is mixed with chitosan oligosaccharide glacial acetic acid solution；(4) feed the mixture into emulsification system emulsified (5) emulsification product carry out cross-linking reaction (6) by washing, precipitating, centrifugation, drying mode handle cross-linking reaction product; obtain the chitosan microball for being loaded with Chinese medical extract; the preparation method is simple, is suitble to the needs of scale industrial production.
Description
Technical field
The present invention relates to a kind of preparation method of Chinese medicine composition, especially a kind of prevention and treatment bacterial enteritis of aquatic livestock
The preparation method of Chinese medicine composition.
Background technique
Fish bacterial enteritis disease is one of the most common type fish disease, and it is conditioned pathogen that cause of disease, which is the dotted gas vacuole bacterium of visible peristalsis visible intestinal peristalsis,
The aquatic livestocks such as main harm grass carp, black carp, silver carp, flathead.Grass carp, black carp may all be infected from fingerling to adult fish, this is died of illness
Rate height is died, for general death rate 50% or so, the serious fishpond death rate of falling ill may be up to 90% or more.General fish bacterial
Enteritis disease comes into vogue more than 18 DEG C of water temperatures, and when epidemic peak is 25-30 DEG C of water temperature, there are two obvious popular seasons in 1 year
Section, the 5-6 month are mainly 1-2 age grass carp, black carp, and the 8-9 month is mainly the morbidity of current year grass carp, and there is generation in national area of respectively breeding fish,
It is to endanger one of serious disease in China's raising fish.Fish bacterial enteritis sick normal and bacterial gill, red skin disease are simultaneously
Hair, therefore also it is referred to as " old three disease ", cardinal symptom is that disease fish appetite stimulator is not ingested even completely, body colour blacks, travelling is slow
Slowly, disease early stage splits the visible intestinal wall contrafluxion inflammation of intestinal tube, has a small amount of food without food or back segment in enteron aisle, after disease
Phase enteron aisle be in aubergine, intestinal wall poor flexibility, enteral without food and mucus is more, anus redness evagination, fish body is intraperitoneal when serious has
Flaxen ascites has carried sick fish head portion, that is, has yellow mucus to flow out from anus.
In short, fish bacterial enteritis is by the factors collective effect such as infectious agent, nutritional deficiency, feed management be bad
It is caused, the growth and production performance of fish are seriously affected, accompanying infection property disease causes fish massive mortality, economic loss
Seriously.Antibiotic is the choice drug for treating such intestinal tract disease, but residual due to the drug resistance of antibiotic and drug
Problem is stayed to become increasingly conspicuous, a series of Chinese herbal and crude drugs preparations have also been developed in the country in recent years, have preferable treatment to fish bacterial enteritis
Effect, but fish intestines systemic disease treatment it is much more existing the approach administration such as take spice feeding, dipping, splash, dosage is big, medicine
Object is easily destroyed in water environment, and dipping drug bioavailability is low, influences drug effect, the general extremely difficult action of traditional Chinese powder medicine.Cause
This researches and develops a kind of Chinese medicine composition and treats fish bacterial enteritis by effective administration route, has very important production practices
Meaning.
Summary of the invention
Technical problem to be solved by the present invention lies in provide a kind of Chinese traditional medicine composition for preventing and treating bacterial enteritis of aquatic livestock
The preparation method of object.
In order to solve the above technical problems, the present invention proposes following technical scheme:
A kind of preparation method of Chinese medicine composition that preventing and treating bacterial enteritis of aquatic livestock, specific steps are as follows:
(1) purslane, the Chinese bulbul and Cortex Phellodendri are pulverized respectively, heating water mentions, and filters out solution and is concentrated, and is concentrated
The Chinese medical concrete A for being 1.20-1.25 to relative density.
(2) chitosan is dissolved in 1-3% glacial acetic acid solution, W/V ratio is 1-1.8:32, and stirring and dissolving obtains chitosan ice vinegar
Acid solution, wherein whipping temp is 45-55 DEG C, and heating method is steam heating.
(3) Chinese medical concrete A is dissolved in step (2) chitosan oligosaccharide glacial acetic acid solution, V/V ratio is 1:3-3:1, obtains mixed solution
B。
(4) mixture B is slowly added in emulsifier, high speed magnetic stirring 35-50 minutes, V/V ratio is 1:35-45, temperature
PH is adjusted to 7.8-9.2 with pH adjusting agent at 50-55 DEG C by degree control, wherein emulsifying device is the emulsion pot with interlayer, is turned
Fast 1000-2000rpm, after obtaining emulsion C；Ultrapure water is slowly added in emulsion C, V/V ratio is 1:10-30, moderate-speed mixer
To room temperature must be stable emulsion D.
(5) emulsion D is continuously stirred, crosslinking agent glutaraldehyde is added in emulsion D, cross-linking reaction 2.5-3.5 hours, V/
V ratio is 1:10-2:9,
(6) after the completion of cross-linking reaction, low-speed centrifugal (50rpm, 20-30 minutes) stands 30 minutes afterwards, abandons supernatant, must consolidate
Body mixture E.
(7) it is alternately washed precipitation mixture E2-4 times, and is centrifuged with dehydrated alcohol and acetone,
(8) by step (7) drying precipitate, the chitosan microball that yellowish-brown is loaded with Traditional Chinese drug mixture is obtained.
Wherein, above-mentioned purslane, the Chinese bulbul and Cortex Phellodendri according to parts by weight, 59-61 parts of purslane, 19-21 parts of the Chinese bulbul,
19-21 parts of Cortex Phellodendri.
Preferably, the preparation method of the Chinese medicine composition of above-mentioned prevention and treatment bacterial enteritis of aquatic livestock, the specific steps are as follows:
(1) purslane, the Chinese bulbul and Cortex Phellodendri are pulverized respectively, are mixed to prepare herb powder A, carried out water and mention,
Powders A and the ratio of distilled water are 1:10-15, and Extracting temperature is 80 DEG C, and extraction time is 12 hours, and room temperature cooling and standings 24 are small
Shi Hou takes supernatant spare, obtains supernatant B；It is 1.20-1.25 to get Chinese medical concrete C that supernatant, which is concentrated into relative density,.
(2) chitosan is dissolved in 1-3% glacial acetic acid, W/V ratio is 1-1.8:32, and it is molten that stirring and dissolving obtains chitosan glacial acetic acid
Liquid, whipping temp are 45-55 DEG C, and heating method is steam heating.
(3) Chinese medical extract C is dissolved in step (6) chitosan oligosaccharide glacial acetic acid solution, V/V ratio is 1:3-3:1, must be mixed molten
Liquid D.
(4) mixture D is slowly added to emulsify in emulsifier, high speed magnetic stirring 35-50 minutes, V/V ratio is 1:
35-45, temperature are controlled at 50-55 DEG C, and pH is adjusted to 7.8-9.2 with pH adjusting agent, and emulsifying device is the emulsion pot with interlayer,
After obtaining emulsion E, ultrapure water is slowly added in emulsion E by revolving speed 1000-2000rpm, and V/V ratio is 1:20-35, and middling speed is stirred
Mix to room temperature must be stable emulsion F.
(5) emulsion F is continuously stirred, crosslinking agent glutaraldehyde is added in emulsion F, cross-linking reaction 2.5-3.5 hours, V/
V ratio is 1:10-2:9.
(6) after the completion of cross-linking reaction, low-speed centrifugal (50rpm, 20-30 minutes) stands 30 minutes afterwards, abandons supernatant, must consolidate
Body mixture G.
(7) it is alternately washed precipitation solid mixture G2-4 times, and is centrifuged with dehydrated alcohol and acetone,
(8) it is dried in vacuo under the conditions of 35-45 DEG C, obtains the chitosan microball that yellowish-brown is loaded with Traditional Chinese drug mixture.
Wherein, above-mentioned purslane, the Chinese bulbul and Cortex Phellodendri according to parts by weight, 59-61 parts of purslane, 19-21 parts of the Chinese bulbul,
19-21 parts of Cortex Phellodendri.
Preferably, above-mentioned for preventing and treating the preparation method of the Chinese medicine composition of bacterial enteritis of aquatic livestock, feature exists
In: partial size is 50-100 μm after the Chinese herbal ultra-fine powder is broken, crosses 100-300 mesh, closed to save backup.
Preferably, the above-mentioned preparation method for for preventing and treating the Chinese medicine composition of bacterial enteritis of aquatic livestock, it is special
Sign is: the emulsifier is the mixture of one of poly lactide-glycolide acid and Tween 80 or sorbester p17, volume
Than for 98.5:1.5-97.5:2.5.
Preferably, the above-mentioned preparation method for for preventing and treating the Chinese medicine composition of bacterial enteritis of aquatic livestock, it is special
Sign is: the pH adjusting agent is phosphate buffer solution (0.2mol/L NaH2PO4:Na2HPO4For 16:84~5.3:94.7)
Or 0.2mol/L ammonium hydroxide.
Preferably, the above-mentioned preparation method for for preventing and treating the Chinese medicine composition of bacterial enteritis of aquatic livestock, it is special
Sign is: the drying mode is vacuum drying (need to be in 35-45 DEG C of condition) or is freeze-dried.
The beneficial effects of the present invention are:
In side of the present invention, medicinal material of the purslane as dual-purpose of drug and food medically has the function such as clearing heat and detoxicating, QI invigorating qi-restoratives
Effect, extract have the inhibiting effect of height to Escherichia coli, proteus, shigella dysenteriae, typhoid fever, paratyphosum Bacterium.Hoary hair
Father-in-law's bitter, it is cold in nature, return stomach, large intestine channel, have clearing heat and detoxicating, removes heat from the blood and relieve diarrhea, the effect of removing dampness and destroying parasites.Cortex Phellodendri property is bitter, cold, returns
Kidney, stomach, large intestine channel have clearing heat and detoxicating, heat-clearing and damp-drying drug and other effects, and the prescription is mutually synergistic, have clearing heat and expelling damp, convergence
The effect of antidiarrheal, strong stomach.
Select chitosan oligosaccharide as pellet carrier, be because are as follows: itself have certain immunogenicity, body can be stimulated to exempt from
Epidemic disease response；Chitosan oligosaccharide molecule is similar to the harmful microbe receptor structure being present on enteral chrotoplast, can be with certain poison
The surface of element, virus and fungal cell combines, and the adjuvant that can be used as exogenous antigen slows down the absorption of antigen, increases the effect of antigen
Valence, to enhance the cell and humoral immune reaction of animal body；Chitosan oligosaccharide can promote a large amount of increasings of beneficial bacterium such as Bifidobacterium etc.
It grows, and the antibody level of body, the phagocytic activity of activating macrophage can be improved in Bifidobacterium, to influence the immune of body
Function.
In short, the preparation method of the Chinese medicine composition of above-mentioned prevention and treatment bacterial enteritis of aquatic livestock, easy to operate, it is prepared
The chitosan microball for being loaded with Traditional Chinese drug mixture can go directly lesion, and maintain higher drug concentration in inflammation part, especially exist
The alimentary canals target organ such as enteron aisle quick-recovery intestinal microecology balance, inhibition pathogen can improve body immunity, delay fastly
Enteritis symptom is solved, quick, the duration is long, and it is easy to use, it is practical；It is suitble to the needs of scale industrial production
Specific embodiment
Technical solution of the present invention is further described combined with specific embodiments below.
Embodiment 1
A kind of Chinese medicine composition for preventing and treating bacterial enteritis of aquatic livestock, is made of purslane, the Chinese bulbul and Cortex Phellodendri, wherein
Purslane 60g, Chinese bulbul 20.5g, Cortex Phellodendri 19.5g.
The preparation method of the Chinese medicine composition of above-mentioned prevention and treatment bacterial enteritis of aquatic livestock, specific preparation process is as follows:
(1) purslane, the Chinese bulbul and Cortex Phellodendri are pulverized respectively, crosses 200 meshes；
(2) weighing purslane 60g, Chinese bulbul 20.5g, Cortex Phellodendri 19.5g is raw material；
(3) water that 13 times of medicinal material total weight amounts are added after mixing purslane, the Chinese bulbul and Cortex Phellodendri carries out water and mentions, and extracts temperature
Degree is 80 DEG C, and extraction time is to take supernatant spare after 12 hours, room temperature static 24 hours；
(4) supernatant in step (3) is concentrated into relative density is 1.23 to get Chinese medical concrete A.
(5) 1g chitosan is dissolved in 32mL1% glacial acetic acid solution, stirring and dissolving obtains chitosan glacial acetic acid solution, wherein
Whipping temp is 45-55 DEG C, and heating method is steam heating.
(6) 32mL Chinese medical concrete A is dissolved in step (5) chitosan oligosaccharide glacial acetic acid solution, obtains mixed solution B.
(7) 64mL mixture is slowly added in emulsifier, high speed magnetic stirring 30 minutes, emulsifier volume is
2560mL, temperature are controlled at 50-55 DEG C, and pH is adjusted to 7.8 with pH adjusting agent, wherein emulsifying device is the emulsification with interlayer
Pot, revolving speed 1000rpm, after obtaining emulsion C；262.4mL ultrapure water is slowly added in emulsion C, moderate-speed mixer to room temperature obtains
Stable emulsion D.
(8) emulsion D is continuously stirred, crosslinking agent glutaraldehyde is added in emulsion D, cross-linking reaction 3 hours, crosslinking agent body
Product is 288.6mL.
(9) after the completion of cross-linking reaction, low-speed centrifugal (50rpm, 20-30 minutes) stands 30 minutes afterwards, abandons supernatant, must consolidate
Body mixture E.
(10) it is alternately washed precipitation mixture E3 times, and is centrifuged with dehydrated alcohol and acetone.
(11) step (10) sediment is freeze-dried, obtains the chitosan microball that yellowish-brown is loaded with Traditional Chinese drug mixture.
Wherein, emulsifier component is made of 98% poly lactide-glycolide acid and 2% Tween 80.
Embodiment 2
A kind of Chinese medicine composition for preventing and treating bacterial enteritis of aquatic livestock, is made of purslane, the Chinese bulbul and Cortex Phellodendri, wherein
Purslane 61g, Chinese bulbul 19g, Cortex Phellodendri 19g.
The preparation method of the Chinese medicine composition of above-mentioned prevention and treatment bacterial enteritis of aquatic livestock, specific preparation process is as follows:
(1) purslane, the Chinese bulbul and Cortex Phellodendri are pulverized respectively, crosses 300 meshes；
(2) weighing purslane 61g, Chinese bulbul 19g, Cortex Phellodendri 19g is raw material；
(3) water that 15 times of medicinal material total weight amounts are added after mixing purslane, the Chinese bulbul and Cortex Phellodendri carries out water and mentions, and extracts temperature
Degree is 80 DEG C, and extraction time is to take supernatant spare after 12 hours, room temperature static 24 hours；
(4) supernatant in step (3) is concentrated into relative density is 1.20 to get Chinese medical concrete A.
(5) 7.5g chitosan is dissolved in 160mL2% glacial acetic acid, stirring and dissolving obtains chitosan glacial acetic acid solution, stirring temperature
Degree is 45 DEG C, and heating method is steam heating.
(6) 160mL Chinese medical extract A is dissolved in step (5) chitosan oligosaccharide glacial acetic acid solution, obtains mixed solution B.
(7) above-mentioned 100mL mixture B is taken to be slowly added to be emulsified in emulsifier, high speed magnetic stirring 40 minutes, emulsification
Agent volume is 4500mL, and temperature is controlled at 50 DEG C, and pH is adjusted to 8 with pH adjusting agent, and emulsifying device is the emulsion pot with interlayer,
After obtaining emulsion C, 131mL ultrapure water is slowly added in emulsion C by revolving speed 2000rpm, and moderate-speed mixer to room temperature must be stable
Emulsion D.
(8) emulsion D is continuously stirred, crosslinking agent glutaraldehyde is added in emulsion D, cross-linking reaction 2.8 hours, glutaraldehyde
Additive amount is 1051.3mL.
(9) after the completion of cross-linking reaction, low-speed centrifugal (50rpm, 20-30 minutes) stands 30 minutes afterwards, abandons supernatant, must consolidate
Body mixture E.
(10) it is alternately washed precipitation mixture E2 times, and is centrifuged with dehydrated alcohol and acetone.
(11) it is dried in vacuo under the conditions of 35-45 DEG C, obtains the chitosan microball that yellowish-brown is loaded with Traditional Chinese drug mixture.
Wherein, emulsifier component is made of 98.5% poly lactide-glycolide acid and 1.5% Tween 80
's.
Embodiment 3
A kind of Chinese medicine composition for preventing and treating bacterial enteritis of aquatic livestock, is made of purslane, the Chinese bulbul and Cortex Phellodendri, wherein
Purslane 59g, Chinese bulbul 21g, Cortex Phellodendri 21g.
The preparation method of the Chinese medicine composition of above-mentioned prevention and treatment bacterial enteritis of aquatic livestock, specific preparation process is as follows:
(1) purslane, the Chinese bulbul and Cortex Phellodendri are pulverized respectively, is sieved with 100 mesh sieve；
(2) weighing purslane 59g, Chinese bulbul 21g, Cortex Phellodendri 21g is raw material；
(3) water that 10 times of medicinal material total weight amounts are added after mixing purslane, the Chinese bulbul and Cortex Phellodendri carries out water and mentions, and extracts temperature
Degree is 80 DEG C, and extraction time is to take supernatant spare after 12 hours, room temperature static 24 hours；
(4) supernatant in step (3) is concentrated into relative density is 1.25 to get Chinese medical concrete A.
(5) 1.8g chitosan is dissolved in 32mL3% glacial acetic acid, stirring and dissolving obtains chitosan glacial acetic acid solution, whipping temp
It is 55 DEG C, heating method is steam heating.
(6) 64mL Chinese medical extract A is dissolved in step (5) chitosan oligosaccharide glacial acetic acid solution, obtains mixed solution B.
(7) 96mL mixture B is taken to be slowly added to be emulsified in emulsifier, high speed magnetic stirring 50 minutes, emulsifier body
Product is 3360mL, and temperature is controlled at 55 DEG C, pH is adjusted to 9.2 with pH adjusting agent, emulsifying device is the emulsion pot with interlayer, is turned
After obtaining emulsion C, 99mL ultrapure water is slowly added in emulsion C by fast 1200rpm, and V/V ratio is 1:20-1:35, moderate-speed mixer
To room temperature must be stable emulsion D.
(8) emulsion D is continuously stirred, crosslinking agent glutaraldehyde is added in emulsion D, cross-linking reaction 3.5 hours, glutaraldehyde
Additive amount is 400mL.
(9) after the completion of cross-linking reaction, low-speed centrifugal (50rpm, 20-30 minutes) stands 30 minutes afterwards, abandons supernatant, must consolidate
Body mixture E.
(10) it is alternately washed precipitation mixture E3 times, and is centrifuged with dehydrated alcohol and acetone.
(11) it is dried in vacuo under the conditions of 35-45 DEG C, obtains the chitosan microball that yellowish-brown is loaded with Traditional Chinese drug mixture.
Wherein, emulsifier component is made of 97.8% poly lactide-glycolide acid and 2.2% Tween 80
's.
Embodiment 4
A kind of Chinese medicine composition for preventing and treating bacterial enteritis of aquatic livestock, is made of purslane, the Chinese bulbul and Cortex Phellodendri, wherein
Purslane 59.5g, Chinese bulbul 20.5g, Cortex Phellodendri 20g.
The preparation method of the Chinese medicine composition of above-mentioned prevention and treatment bacterial enteritis of aquatic livestock, specific preparation process is as follows:
(1) purslane, the Chinese bulbul and Cortex Phellodendri are pulverized respectively, crosses 140 meshes；
(2) weighing purslane 59.5g, Chinese bulbul 20.5g, Cortex Phellodendri 20g is raw material；
(3) water that 14 times of medicinal material total weight amounts are added after mixing purslane, the Chinese bulbul and Cortex Phellodendri carries out water and mentions, and extracts temperature
Degree is 80 DEG C, and extraction time is to take supernatant spare after 12 hours, room temperature static 24 hours；
(4) supernatant in step (3) is concentrated into relative density is 1.22 to get Chinese medical concrete A.
(5) 3.6g chitosan is dissolved in 64mL3% glacial acetic acid, stirring and dissolving obtains chitosan glacial acetic acid solution, whipping temp
It is 45 DEG C, heating method is steam heating.
(6) 64mL Chinese medical extract A is dissolved in step (5) chitosan oligosaccharide glacial acetic acid solution, obtains mixed solution B.
(7) mixture B is slowly added to emulsify in emulsifier, high speed magnetic stirring 35-50 minutes, emulsifier volume
For 5120mL, temperature is controlled at 55 DEG C, pH is adjusted to 8.0 with pH adjusting agent, emulsifying device is the emulsion pot with interlayer, revolving speed
After obtaining emulsion C, 175mL ultrapure water is slowly added in emulsion C by 1000rpm, and V/V ratio is 1:20-1:35, moderate-speed mixer
To room temperature must be stable emulsion D.
(8) emulsion D is continuously stirred, crosslinking agent glutaraldehyde is added in emulsion D, cross-linking reaction 3 hours, glutaraldehyde added
Dosage is 813mL.
(9) after the completion of cross-linking reaction, low-speed centrifugal (50rpm, 20-30 minutes) stands 30 minutes afterwards, abandons supernatant, must consolidate
Body mixture E.
(10) it is alternately washed precipitation mixture E2 times, and is centrifuged with dehydrated alcohol and acetone.
(11) it is freeze-dried, obtains the chitosan microball that yellowish-brown is loaded with Traditional Chinese drug mixture.
Wherein, emulsifier component is made of 98.5% poly lactide-glycolide acid and 1.5% Tween 80
's.
Chinese medicine described in one of embodiment 1-4 is commercial product, and dosage of each component is added deduct according to same ratio increasing
Few, the parts by weight relationship of gained each component all belongs to the scope of protection of the present invention.
Chinese medicine described in one of embodiment 1-4 meets " Chinese veterinary pharmacopoeia " two ministerial standard of version in 2010.
The composition principle of Chinese medicine composition of the present invention is: purslane enters the heart, liver, spleen, large intestine channel, heat-clearing solution in side
Poison, Li Shui are dried, are dissipated blood detumescence, dust-collection sterilization, anti-inflammatory analgetic, blood-stanching and blood-cooling；The Chinese bulbul returns stomach, large intestine channel, has heat-clearing solution
Poison, remove heat from the blood and relieve diarrhea, removing dampness and destroying parasites the effect of；Cortex Phellodendri returns kidney, bladder, large intestine channel, heat-clearing and damp-drying drug, purging fire for removing toxin, except hectic fever due to yin is empty clearly
Heat.All medicines share, it is mutually synergistic, it is prominent to prevention and treatment bacterial enteritis of aquatic livestock effect, especially with described in embodiment 1
Extremely significant, the prevention and treatment of particularly suitable bacterial enteritis of aquatic livestock of the effect of proportion, anti-inflammatory analgetic, purging fire for removing toxin.
Clinical test example:
10 mu of Tianjin Jinghai County crawl, 1 meter of the depth of water, there is sick fish, sick fish belly in August, 2012, pond culture grass carp
Portion is expanded, and erythema is presented, and anus is prominent, and fish body is serious the fish phenomena of mortality occur in the symptoms such as dull.Analyze reason of catching an illness
Are as follows: as temperature is gradually increasing, fish growth is very fast, and various pathogenic bacteria increase sharply；Furthermore unqualified feed or high protein are fed for a long time
Feed causes fish nutrition metabolism bad, constitution decline, body fat accumulation；Antibiotic is added in feed a part, results in hepatopathy
Become；Condition of water quality deteriorates for a long time, and hydrogen sulfide, ammoniacal nitrogen severely exceed in pond, so that fish slow poisoning.
Then it is treated using Chinese medicine composition described in embodiment 1, the 50ml Chinese traditional medicine composition is uniformly poured into crawl
Object (5ml/ mus of water bodys), after 3 days, sampling inspection fish diseased region, it is obviously good that sampling results show that fish diseased region has
Turning, improvement rate is continued the prevention of bacillary enteritis using the Chinese medicine composition afterwards, used each moon once up to 98%,
Disease incidence reduces 15% compared with the same period in former years.
Above-mentioned referring to embodiment is to the detailed of the preparation method of the Chinese medicine composition of the prevention and treatment bacterial enteritis of aquatic livestock
Thin description, is illustrative without being restrictive, several embodiments can be enumerated according to limited range, therefore do not taking off
From the change and modification under present general inventive concept, should belong within protection scope of the present invention.
Claims (5)
1. a kind of preparation method for the Chinese medicine composition for preventing and treating bacterial enteritis of aquatic livestock, it is characterised in that: specific steps are as follows:
(1) purslane, the Chinese bulbul and Cortex Phellodendri are pulverized respectively, heating water mentions, and filters out solution and is concentrated, is concentrated into phase
The Chinese medical concrete A for being 1.20-1.25 to density；
(2) chitosan is dissolved in 1-3% glacial acetic acid solution, W/V ratio is 1-1.8:32, and it is molten that stirring and dissolving obtains chitosan glacial acetic acid
Liquid, wherein whipping temp is 45-55 DEG C, and heating method is steam heating；
(3) Chinese medical concrete A is dissolved in step (2) chitosan oligosaccharide glacial acetic acid solution, V/V ratio is 1:3-3:1, obtains mixed solution B；
(4) mixture B is slowly added in emulsifier, high speed magnetic stirring 35-50 minutes, V/V ratio is 1:35-45, temperature control
PH is adjusted to 7.8-9.2 with pH adjusting agent at 50-55 DEG C by system, wherein emulsifying device is the emulsion pot with interlayer, revolving speed
1000-2000rpm, after obtaining emulsion C；Ultrapure water is slowly added in emulsion C, V/V ratio is 1:10-30, and moderate-speed mixer is extremely
Room temperature must be stable emulsion D；
(5) emulsion D is continuously stirred, crosslinking agent glutaraldehyde is added in emulsion D, cross-linking reaction 2.5-3.5 hours, V/V ratio
For 1:10-2:9；
(6) after the completion of cross-linking reaction, 30 minutes are stood after low-speed centrifugal, supernatant is abandoned, obtains solid mixture E；
(7) it is alternately washed precipitation mixture E2-4 times, and is centrifuged with dehydrated alcohol and acetone；
(8) by step (7) drying precipitate, the chitosan microball that yellowish-brown is loaded with Traditional Chinese drug mixture is obtained；
Wherein, above-mentioned purslane, the Chinese bulbul and Cortex Phellodendri according to parts by weight, 59-61 parts of purslane, 19-21 parts of the Chinese bulbul, Cortex Phellodendri
19-21 parts.
2. it is according to claim 1 for preventing and treating the preparation method of the Chinese medicine composition of bacterial enteritis of aquatic livestock,
Be characterized in that: partial size is 50-100 μm after the Chinese herbal ultra-fine powder is broken, crosses 100-300 mesh, closed to save backup.
3. it is according to claim 1 or 2 for preventing and treating the preparation method of the Chinese medicine composition of bacterial enteritis of aquatic livestock,
It is characterized by: the emulsifier is the mixture of one of poly lactide-glycolide acid and Tween 80 or sorbester p17,
Volume ratio is 98.5:1.5-97.5:2.5.
4. it is according to claim 1 or 2 for preventing and treating the preparation method of the Chinese medicine composition of bacterial enteritis of aquatic livestock,
It is characterized by: the pH adjusting agent is phosphate buffer solution or 0.2mol/L ammonium hydroxide.
5. it is according to claim 1 or 2 for preventing and treating the preparation method of the Chinese medicine composition of bacterial enteritis of aquatic livestock,
It is characterized by: the drying mode is vacuum drying or freeze-drying.
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|---|---|---|---|---|
| CN101422526A (en) * | 2007-11-02 | 2009-05-06 | 天津瑞普生物技术集团有限公司 | Traditional Chinese medicine oral liquid for treating poultry colibacillosis |
| WO2012108892A1 (en) * | 2011-02-09 | 2012-08-16 | Seubert Kirk | Combinations of berberine, artemisinin, loperamide and their derivatives to treat malaria, diarrhea, travelers' diarrhea, dysentery, dengue fever, parasites, cholera and viruses |
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