CN105259165A - Reagent feeding device of DNA sequenator - Google Patents
Reagent feeding device of DNA sequenator Download PDFInfo
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- CN105259165A CN105259165A CN201510706066.7A CN201510706066A CN105259165A CN 105259165 A CN105259165 A CN 105259165A CN 201510706066 A CN201510706066 A CN 201510706066A CN 105259165 A CN105259165 A CN 105259165A
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- platform
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- assembly
- web joint
- leading screw
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Abstract
The invention relates to a reagent feeding device of a DNA sequenator. The reagent feeding device comprises a liquid box lifting platform assembly, the liquid box lifting platform assembly is an up-down reciprocating motion mechanism driven by a motor lead screw and comprises a driving motor, a supporting base, a lead screw assembly and a reaction liquid box lifting base, wherein the supporting base is connected with the driving motor and used for supporting the driving motor, the lead screw assembly is connected with the driving motor, and the reaction liquid box lifting base is installed on the side part. The reaction liquid box lifting base is internally provided with a buffering liquid box loading and unloading platform assembly of a drawing structure. After reagents are arranged on a platform, the whole platform assembly is installed on a right side box body, when the reagents need to be loaded or unloaded, the platform is drawn out, and after the reagents are placed in place, the platform is pushed to the original position. The liquid box lifting platform assembly conveys the reagents to the appointed position through guide rail motion and is convenient and accurate to control. The liquid box lifting platform conveys a pump valve integrated pipe assembly and the liquid box lifting platform assembly vertically to complete the conveying and reaction process of the reagents.
Description
Technical field
The present invention relates to DNA sequencing field, particularly relate to a kind of can load and promote transport the agent delivery device of DNA sequencer.
Background technology
In DNA sequencing process, chemical reaction occurs sequence testing chip, produce visible ray, the light signal produced by CCD (ChargeCoupleDevice) cameras capture sequencing reaction, can obtain required order-checking information.
Agent delivery system provides plurality of reagents in reaction chip, requires that DNA sequencer can store, carries according to demand, and be convenient for changing reagent; Store, conveying and change reagent process in, also must conveniently control, so as supply reagent and reaction warehouse in sequence testing chip continue to react, complete sequencing procedure.
In view of above-mentioned defect, creator of the present invention obtains this creation finally through long research and practice.
Summary of the invention
The object of the present invention is to provide a kind of agent delivery device of DNA sequencer, in order to overcome above-mentioned technological deficiency.
For achieving the above object, the invention provides a kind of agent delivery device of DNA sequencer, comprise liquid case hoistable platform assembly, its mechanism that pumps driven for motor leading screw; It comprises drive motor, be connected with drive motor and the supporting seat of support drive motor, the lead screw assembly be connected with drive motor, be arranged on the reaction liquid case elevating bracket of sidepiece, wherein, damping fluid case loading/unloading platform assembly is equiped with in described reaction liquid case elevating bracket;
Described damping fluid case loading/unloading platform assembly is drawing and pulling type structure, and after platform sets reagent, whole platform assembly is arranged in the casing of right side, and when needs handling reagent, pulled out by platform, after placing, pushed home is put again;
Described damping fluid case loading/unloading platform assembly comprises carrier fluid platform, its be one with handle arm-tie, and handle be fixed on handle plate; The both sides of carrier fluid platform are provided with web joint, and the horizontal part of web joint is connected with liquid case elevating bracket with reaction; Between described carrier fluid platform and the web joint of both sides, arrange slide track component, it makes carrier fluid platform move along web joint.
Further, described reaction liquid case elevating bracket comprises the intermediate plate be connected with installing plate, is arranged on the supporting plate of intermediate plate both sides, supporting plate is provided with horizontal ends, in order to place damping fluid case loading/unloading platform assembly.
Further, the output shaft of described drive motor is connected with leading screw, and leading screw is arranged in base, and base is connected by supporting seat with the end of drive motor; The sidepiece of base is provided with stroke detection switch;
The output shaft of described drive motor is connected with the link of leading screw by shaft coupling; Leading screw is connected with nut, and nut is fixed on nut seat; Leading screw lower end is by bearings, and bearing end is provided with bearing (ball) cover.
Further, be also provided with guide rail in the inner side of described base, reaction liquid case elevating bracket is arranged on guide rail slide block, with slide block movement; Reaction liquid case elevating bracket is provided with position check-out console, base is provided with position transducer, it is arranged on position transducer mount pad;
Described position transducer mount pad comprises installing plate, it is arranged installation threaded hole, is connected by screw with base; Also comprise the second check-out console and sensor installing plate, slideway is set at sensor installing plate and the second check-out console, with for described position check-out console mistake, carry out location sensitive.
Further, described base is a frame-type structure, leading screw through hole on upper surface is offered, and lower leading screw through hole is offered in lower surface, installs bearing supporting wire thick stick in two through hole; Be mounting flange in base lower end, on the right side of itself and sequenator, the base assembly of casing is connected and fixed; The sidepiece of base is provided with guide rails assembling face, it offers guide rails assembling threaded hole.
Further, described slide track component comprises the clamp being fixed on web joint sidepiece, and relative clamp moving also can coordinate the slide plate stopped with clamp, slide plate is fixed on the sidepiece of platform web joint, platform web joint is arranged on the lower end of carrier fluid platform, and it drives carrier fluid platform movement.
Further, described clamp arranges slideway, slide plate can move on slideway; The slip of clamp arranges stop aperture, when the projection on slide plate is plugged in stop aperture, slide plate and the stop motion of connected carrier fluid platform;
Platform web joint and web joint have identical structure, and when mounted, both oppositely install; Its cross section is wedge shape, comprises the surface level of a dip plane and sidepiece, and surface level is in order to be connected and fixed.
Compared with prior art beneficial effect of the present invention is: the liquid case hoistable platform assembly of the agent delivery device of DNA sequencer of the present invention by guide rail movement by reagent delivery to assigned address, it is convenient, precisely to control; Pump valve integrated pipe assembly and damping fluid case loading/unloading platform assembly transport by liquid case hoistable platform up and down, complete reagent conveying, course of reaction.
Adopt pump valve integrated pipe assembly, can select plurality of reagents simultaneously, and carry out sequencing reaction according to the order pre-set, testing efficiency is high.Damping fluid case loading/unloading platform assembly, not only can provide the reagent of demand, and handling reagent is convenient, saves cabinet space.
Accompanying drawing explanation
Fig. 1 is the perspective view of liquid case hoistable platform assembly of the present invention;
Fig. 2 is the side-looking structural representation of liquid case hoistable platform assembly of the present invention;
Fig. 3 a is the side-looking structural representation of base of the present invention;
Fig. 3 b is the structural representation of position transducer mount pad of the present invention;
Fig. 3 c is the structural representation of position transducer mount pad of the present invention;
Fig. 3 d is the structural representation of supporting seat of the present invention;
Fig. 3 e is the perspective view of nut mount pad of the present invention;
Fig. 3 f is the perspective view of base of the present invention;
Fig. 4 a is the perspective view of pump valve integrated pipe assembly of the present invention;
Fig. 4 b is the backsight structural representation of pump valve integrated pipe assembly of the present invention;
Fig. 4 c is the plan structure schematic diagram of pump valve integrated pipe assembly of the present invention;
Fig. 4 d is the structural representation of pump valve integrated base plate of the present invention;
Fig. 5 a is the perspective view of damping fluid case loading/unloading platform assembly of the present invention;
Fig. 5 b be damping fluid case loading/unloading platform assembly of the present invention face structural representation;
Fig. 5 c is the structural representation in the guide rail cross section of damping fluid case loading/unloading platform assembly of the present invention;
Fig. 5 d is the structural representation of the web joint of damping fluid case loading/unloading platform assembly of the present invention;
Fig. 5 e is the structural representation of the handle plate of damping fluid case loading/unloading platform assembly of the present invention;
Fig. 5 f is the structural representation of slideway of the present invention.
Embodiment
Below in conjunction with accompanying drawing, to above-mentioned being described in more detail with other technical characteristic and advantage of the present invention.
The agent delivery device of DNA sequencer of the present invention, also can be transferred to relevant position up and down by load reagents, be transferred in reaction warehouse and chemical reaction occurs, complete sequencing procedure by reagent pipeline.
Refer to shown in Fig. 1 and 2, the agent delivery device of the embodiment of the present invention comprises liquid case hoistable platform assembly, its mechanism that pumps driven for motor leading screw, and it is arranged in the right side casing of sequenator.It comprises drive motor 401, be connected with drive motor and the supporting seat 402 of support drive motor, the lead screw assembly be connected with drive motor, be arranged on the reaction liquid case elevating bracket 408 of sidepiece, wherein, damping fluid case loading/unloading platform assembly is equiped with in described reaction liquid case elevating bracket 408, reaction is arranged on the installing plate 404 that is connected with reaction liquid case elevating bracket with liquid case elevating bracket 408, under the driving of screw mechanism, carrying reaction liquid case elevating bracket Reagent Tube group moves up and down.
Reaction comprises the intermediate plate 4081 be connected with installing plate with liquid case elevating bracket 408, be arranged on the supporting plate 4082 of intermediate plate both sides, supporting plate 4082 is provided with horizontal ends, in order to place damping fluid case loading/unloading platform assembly.
The output shaft of described drive motor 401 is connected with leading screw 403, and leading screw is arranged in base 405, and base 405 is connected by supporting seat 402 with the end of drive motor 401; The sidepiece of base 405 is provided with stroke detection switch 406.
The output shaft of described drive motor is connected with the link 4034 of leading screw by shaft coupling 4035; Leading screw is connected with nut 4031, nut is fixed on nut seat 4032; Leading screw lower end is by bearings, and bearing end is provided with bearing (ball) cover 4011.
The liquid case hoistable platform assembly of the embodiment of the present invention by guide rail movement by reagent delivery to assigned address, it is convenient, precisely to control; Pump valve integrated pipe assembly and damping fluid case loading/unloading platform assembly transport by liquid case hoistable platform up and down, complete reagent conveying, course of reaction.
Refer to shown in Fig. 3 a-3f, be also provided with guide rail 407 in the inner side of base, reaction liquid case elevating bracket 408 is arranged on guide rail slide block, with slide block movement; Reaction liquid case elevating bracket 408 is provided with position check-out console 4071, and base is provided with position transducer, and it is arranged on position transducer mount pad 4072.Shown in Fig. 3 b, it is the structural representation of position transducer mount pad of the present invention, and it comprises installing plate 4076, it is arranged and installs threaded hole 4077, be connected by screw with base; Second check-out console 4074, sensor installing plate 4075, arranges slideway 4073 at sensor installing plate 4075 and the second check-out console 4074, passes through, carry out location sensitive with for described position check-out console 4071.
Refer to shown in Fig. 3 c, the position check-out console of the embodiment of the present invention comprises top installing plate 4081, by being arranged on screw in threaded hole 4082 and reaction liquid case elevating bracket 408 is connected and fixed; Also comprise the position detection part 4083 be connected, in order to respond to mutually with position transducer.
Refer to shown in Fig. 3 d, described supporting seat 402 comprises connecting plate for electric motor 4021, and it is connected with motor end face, it is arranged connecting thread hole 4022; The leading screw sleeve 4023 be connected, is provided with locating surface 4024 in leading screw sleeve, in order to install leading screw.
Refer to shown in Fig. 3 e, nut mount pad comprises nut installed surface 4114, it is arranged lead screw hole 4113, and the surrounding of nut installed surface 4114 arranges fastening screw nail; The installed surface 4112 be connected with elevating bracket installing plate 404 is set in upper surface.
Referring to shown in Fig. 3 f, the base 405 of the embodiment of the present invention, is a frame-type structure, leading screw through hole 4051 on upper surface is offered, and lower leading screw through hole 4054 is offered in lower surface, installs bearing supporting wire thick stick in two through hole; Be mounting flange 4053 in base lower end, on the right side of itself and sequenator, the base assembly of casing is connected and fixed; The sidepiece 4052 of base is provided with guide rails assembling face, it offers guide rails assembling threaded hole.
The embodiment of the present invention adopts feed screw nut to carry out reciprocal transportation to reagent, the control accuracy of leading screw, can meet the delivery requirements of reagent.
Refer to Fig. 4 a-4d to show, reagent is sucked by the syringe needle suction pipe 507 of downside by the pump valve integrated pipe assembly of the embodiment of the present invention from the Reagent Tube damping fluid case loading/unloading platform assembly, and pass through the selection of fluid path control module 502, drived control valve module 509 and hydraulic pump 506 are selected, reagent is entered in reagent cartridge, carry out sequencing reaction.
Described pump valve integrated pipe assembly comprises pump valve integrated base plate 508, pump valve integrated base plate 508 comprises peristaltic pump installing plate 5011, the fixed head 5083 be connected with peristaltic pump installing plate 5011, base plate 5084, is arranged on the sipper pore group 5085 on base plate and the operation valve unit installing board 5081 in the middle of base plate; Peristaltic pump installing plate 5011 is provided with peristaltic pump mounting hole 5082, it is arranged two peristaltic pumps 505; On base plate 5084, also interval arranges multiple reagent pipe clamp and wire clamp 5015.
Accordingly, operation valve assembly 509 is arranged on the center section of pump valve integrated base plate 508, and hydraulic pump 506 is arranged on its sidepiece, arranges fluid path control module 502 in the other side, and fluid path control module 502 is isolated with fluid path part by a bending plate 503.
In embodiments of the present invention, in order to carry out sequencing reaction to plurality of reagents, the sipper pore group 5085 on base plate has been arranged side by side multiple hole, and syringe needle suction pipe 507 is through sipper pore, and the other end connects Reagent Tube and is connected with the fluid path port of corresponding operation valve; Sipper pore arranges pipe clamp 5013, prevents syringe needle suction pipe from tilting.
The embodiment of the present invention, adopt pump valve integrated pipe assembly, can select plurality of reagents simultaneously, and carry out sequencing reaction according to the order pre-set, testing efficiency is high.
Refer to shown in Fig. 5 a-5f, damping fluid case loading/unloading platform assembly is the structure of drawing and pulling type, and after platform sets reagent, whole platform assembly is arranged in the casing of right side, and when needs handling reagent, pulled out by platform, after placing, pushed home is put again.
It comprises carrier fluid platform 310, and in the present embodiment, it is one with the arm-tie of handle 3101, and handle is fixed on handle plate 3102; The both sides of carrier fluid platform 310 are provided with web joint 311, and the horizontal part of web joint 311 is connected with the supporting plate 4082 of reaction with liquid case elevating bracket 408; Under the effect of reaction with liquid case elevating bracket 408, carrier fluid platform 310 moves up to syringe needle suction pipe 507 place on the downside of pump valve integrated pipe, through the control of control module, selectively draws reagent.
Between described carrier fluid platform 310 and the web joint 311 of both sides, arrange slide track component 312, it makes carrier fluid platform move along web joint; It comprises the clamp 315 being fixed on web joint 311 sidepiece, move relative to clamp 315 and the slide plate stopped can be coordinated with clamp, slide plate is fixed on the sidepiece of platform web joint 316, and platform web joint 316 is arranged on the lower end of carrier fluid platform, and it drives carrier fluid platform movement.
Refer to shown in Fig. 5 f, clamp 315 is arranged slideway 3105, slide plate can move on slideway 3105; The slip 3105 of clamp 315 arranges stop aperture 3104, when the projection on slide plate is plugged in stop aperture 3104, slide plate and the stop motion of connected carrier fluid platform.
Platform web joint 316 has identical structure with web joint 311, and when mounted, both oppositely install; Its cross section is wedge shape, and comprise the surface level 3112 of a dip plane and sidepiece, surface level 3112 is in order to be connected and fixed.
The damping fluid case loading/unloading platform assembly of the embodiment of the present invention, not only can provide the reagent of demand, and handling reagent is convenient, saves cabinet space.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and this embodiment is also not used to limit the scope of the claims of the present invention, and the equivalence that all the present invention of disengaging do is implemented or changed, and all should be contained in the scope of technical solution of the present invention.
Claims (7)
1. an agent delivery device for DNA sequencer, is characterized in that, comprises liquid case hoistable platform assembly, its mechanism that pumps driven for motor leading screw; It comprises drive motor, be connected with drive motor and the supporting seat of support drive motor, the lead screw assembly be connected with drive motor, be arranged on the reaction liquid case elevating bracket of sidepiece, wherein, damping fluid case loading/unloading platform assembly is equiped with in described reaction liquid case elevating bracket;
Described damping fluid case loading/unloading platform assembly is drawing and pulling type structure, and after platform sets reagent, whole platform assembly is arranged in the casing of right side, and when needs handling reagent, pulled out by platform, after placing, pushed home is put again;
Described damping fluid case loading/unloading platform assembly comprises carrier fluid platform, its be one with handle arm-tie, and handle be fixed on handle plate; The both sides of carrier fluid platform are provided with web joint, and the horizontal part of web joint is connected with liquid case elevating bracket with reaction; Between described carrier fluid platform and the web joint of both sides, arrange slide track component, it makes carrier fluid platform move along web joint.
2. the agent delivery device of DNA sequencer according to claim 1, it is characterized in that, described reaction liquid case elevating bracket comprises the intermediate plate be connected with installing plate, is arranged on the supporting plate of intermediate plate both sides, supporting plate is provided with horizontal ends, in order to place damping fluid case loading/unloading platform assembly.
3. the agent delivery device of DNA sequencer according to claim 2, is characterized in that, the output shaft of described drive motor is connected with leading screw, and leading screw is arranged in base, and base is connected by supporting seat with the end of drive motor; The sidepiece of base is provided with stroke detection switch;
The output shaft of described drive motor is connected with the link of leading screw by shaft coupling; Leading screw is connected with nut, and nut is fixed on nut seat; Leading screw lower end is by bearings, and bearing end is provided with bearing (ball) cover.
4. the agent delivery device of DNA sequencer according to claim 2, is characterized in that, is also provided with guide rail in the inner side of described base, and reaction liquid case elevating bracket is arranged on guide rail slide block, with slide block movement; Reaction liquid case elevating bracket is provided with position check-out console, base is provided with position transducer, it is arranged on position transducer mount pad;
Described position transducer mount pad comprises installing plate, it is arranged installation threaded hole, is connected by screw with base; Also comprise the second check-out console and sensor installing plate, slideway is set at sensor installing plate and the second check-out console, with for described position check-out console mistake, carry out location sensitive.
5. the agent delivery device of DNA sequencer according to claim 4, is characterized in that, described base, is a frame-type structure, leading screw through hole on upper surface is offered, and lower leading screw through hole is offered in lower surface, installs bearing supporting wire thick stick in two through hole; Be mounting flange in base lower end, on the right side of itself and sequenator, the base assembly of casing is connected and fixed; The sidepiece of base is provided with guide rails assembling face, it offers guide rails assembling threaded hole.
6. the agent delivery device of DNA sequencer according to claim 1, it is characterized in that, described slide track component comprises the clamp being fixed on web joint sidepiece, relative clamp moving also can coordinate the slide plate stopped with clamp, slide plate is fixed on the sidepiece of platform web joint, platform web joint is arranged on the lower end of carrier fluid platform, and it drives carrier fluid platform movement.
7. the agent delivery device of DNA sequencer according to claim 6, is characterized in that, described clamp arranges slideway, and slide plate can move on slideway; The slip of clamp arranges stop aperture, when the projection on slide plate is plugged in stop aperture, slide plate and the stop motion of connected carrier fluid platform;
Platform web joint and web joint have identical structure, and when mounted, both oppositely install; Its cross section is wedge shape, comprises the surface level of a dip plane and sidepiece, and surface level is in order to be connected and fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510706066.7A CN105259165B (en) | 2015-10-27 | 2015-10-27 | A kind of agent delivery device of DNA sequencer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510706066.7A CN105259165B (en) | 2015-10-27 | 2015-10-27 | A kind of agent delivery device of DNA sequencer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105259165A true CN105259165A (en) | 2016-01-20 |
| CN105259165B CN105259165B (en) | 2017-03-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510706066.7A Expired - Fee Related CN105259165B (en) | 2015-10-27 | 2015-10-27 | A kind of agent delivery device of DNA sequencer |
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| Country | Link |
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| CN (1) | CN105259165B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107916220A (en) * | 2016-10-11 | 2018-04-17 | 广州康昕瑞基因健康科技有限公司 | XY platforms screw drives mounting structure |
| CN110861111A (en) * | 2019-12-03 | 2020-03-06 | 哈尔滨工业大学 | Micro-nano robot control platform with magnetic field and electric field coupling effect |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009022280A2 (en) * | 2007-08-14 | 2009-02-19 | Koninklijke Philips Electronics N.V. | Transport module of a single-cartridge diagnostic device |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN104266885A (en) * | 2014-07-25 | 2015-01-07 | 安徽省一一通信息科技有限公司 | Sample automatic-treatment system |
| CN104345165A (en) * | 2010-07-23 | 2015-02-11 | 贝克曼考尔特公司 | System and method including analytical units |
-
2015
- 2015-10-27 CN CN201510706066.7A patent/CN105259165B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009022280A2 (en) * | 2007-08-14 | 2009-02-19 | Koninklijke Philips Electronics N.V. | Transport module of a single-cartridge diagnostic device |
| CN104345165A (en) * | 2010-07-23 | 2015-02-11 | 贝克曼考尔特公司 | System and method including analytical units |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN104266885A (en) * | 2014-07-25 | 2015-01-07 | 安徽省一一通信息科技有限公司 | Sample automatic-treatment system |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107916220A (en) * | 2016-10-11 | 2018-04-17 | 广州康昕瑞基因健康科技有限公司 | XY platforms screw drives mounting structure |
| CN110861111A (en) * | 2019-12-03 | 2020-03-06 | 哈尔滨工业大学 | Micro-nano robot control platform with magnetic field and electric field coupling effect |
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| Publication number | Publication date |
|---|---|
| CN105259165B (en) | 2017-03-15 |
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