CN105254740B - 一种生物活性多肽nqfyqkf及其制备与应用 - Google Patents
一种生物活性多肽nqfyqkf及其制备与应用 Download PDFInfo
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- CN105254740B CN105254740B CN201510672566.3A CN201510672566A CN105254740B CN 105254740 B CN105254740 B CN 105254740B CN 201510672566 A CN201510672566 A CN 201510672566A CN 105254740 B CN105254740 B CN 105254740B
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- biologically active
- active polypeptide
- milk
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- polypeptide
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4732—Casein
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
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CN106995485B (zh) * | 2017-05-12 | 2020-03-31 | 南京市妇幼保健院 | 一种分离的内源性抗菌多肽及其应用 |
CN107903315A (zh) * | 2017-11-14 | 2018-04-13 | 上海交通大学 | 一种生物活性多肽neinqfyq及其制备方法和应用 |
CN107840882A (zh) * | 2017-11-14 | 2018-03-27 | 上海交通大学 | 一种生物活性多肽dipnpigse及其制备方法和应用 |
CN107840879A (zh) * | 2017-11-14 | 2018-03-27 | 上海交通大学 | 一种生物活性多肽neinqfyqkfpq及其制备方法和应用 |
CN108017703B (zh) * | 2017-12-11 | 2020-07-17 | 浙江辉肽生命健康科技有限公司 | 一种生物活性多肽vpitptln及其制备方法和应用 |
CN107880106B (zh) * | 2017-12-11 | 2020-06-16 | 浙江辉肽生命健康科技有限公司 | 一种生物活性多肽vpitptlnre及其制备方法和应用 |
CN110759970A (zh) * | 2018-07-27 | 2020-02-07 | 中国科学院大连化学物理研究所 | 一种源于发酵牛乳的抗氧化多肽及其应用和添加剂 |
CN110724175B (zh) * | 2019-10-14 | 2021-10-22 | 浙江海洋大学 | 一种利用超声匀浆从紫贻贝加工下脚料中提取抗菌肽的制备方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010106437A1 (en) * | 2009-03-20 | 2010-09-23 | The Governors Of The University Of Alberta | Peptides that inhibit angiotensin converting enzyme and peptides with antioxidant activity purified from ovotransferrin and methods of producing and using the same |
CN103232526A (zh) * | 2012-12-12 | 2013-08-07 | 上海交通大学 | 一种生物活性多肽lplp及其制备和应用 |
CN103232528A (zh) * | 2012-12-12 | 2013-08-07 | 上海交通大学 | 一种生物活性多肽delq及其制备和应用 |
CN104479001A (zh) * | 2014-12-19 | 2015-04-01 | 上海交通大学 | κ-酪蛋白来源生物活性肽的制备和应用 |
-
2015
- 2015-10-16 CN CN201510672566.3A patent/CN105254740B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010106437A1 (en) * | 2009-03-20 | 2010-09-23 | The Governors Of The University Of Alberta | Peptides that inhibit angiotensin converting enzyme and peptides with antioxidant activity purified from ovotransferrin and methods of producing and using the same |
CN103232526A (zh) * | 2012-12-12 | 2013-08-07 | 上海交通大学 | 一种生物活性多肽lplp及其制备和应用 |
CN103232528A (zh) * | 2012-12-12 | 2013-08-07 | 上海交通大学 | 一种生物活性多肽delq及其制备和应用 |
CN104479001A (zh) * | 2014-12-19 | 2015-04-01 | 上海交通大学 | κ-酪蛋白来源生物活性肽的制备和应用 |
Non-Patent Citations (3)
Title |
---|
alpha s2casein[Capra hircus];Ramunno,L.;《GenBank DataBase》;NCBI Blast;20061114;Accession NO:CBA44000.1 * |
Mutational analysis of immunoglobulin E-binding epitopes of beta-casein and beta-lactoglobulin showed a heterogeneous pattern of critical amino acids between individual patients and pooled sera;Cocco RR等;《Clinical and Experimental Allergy》;PUBMED;20070731;第37卷(第6期);第831-838页 * |
生物活性多肽序列鉴定方法的研究;徐海红等;《中国乳品工业》;CNKI;20150731;第43卷(第7期);第4-6页 * |
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