CN105237522A - Method for preparing vitexin with Chinese prickly ash plants as raw materials - Google Patents
Method for preparing vitexin with Chinese prickly ash plants as raw materials Download PDFInfo
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- CN105237522A CN105237522A CN201510670560.2A CN201510670560A CN105237522A CN 105237522 A CN105237522 A CN 105237522A CN 201510670560 A CN201510670560 A CN 201510670560A CN 105237522 A CN105237522 A CN 105237522A
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- vitexin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D407/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00
- C07D407/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings
- C07D407/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
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Abstract
The invention discloses a method for preparing vitexin with Chinese prickly ash plants as raw materials, application of preparing vitexin raw materials with all portions of Chinese prickly ash plants, and a method for obtaining vitexin products by performing extraction, separation and purification on different portions of Chinese prickly ash through extracting agents. Chinese prickly ash leaves are by-products of the Chinese prickly ash planting industry. As is found in research of the methods and the application, the content (13.2 mg/g) of vitexin in the Chinese prickly ash leaves is high, is 33 times the content (0.4 mg/g) of vitexin in broussonetia papyrifera, is 264 times the content (0.05 mg/g) of vitexin in hawthorn leaves and is over 4 times the content of vitexin in vitex, and the Chinese prickly ash leaves further contain chlorogenic acid, quinic acid and other ingredients and can serve as new raw material sources for producing vitexin and greatly reduce production cost of vitexin. A new path is provided for comprehensively utilizing the Chinese prickly ash leaves, and the social, economical and environmental benefits of the Chinese prickly ash industry and the vitexin industry can be improved.
Description
Technical field
The present invention relates to a kind of novelty teabag of Chinese prickly ash plant parts.Specifically Chinese prickly ash plant different sites is as the separation purification method of the application and Vitexin of preparing Vitexin raw material.
Background technology
Diseases of cardiovascular and cerebrovascular systems is the most common class diseases of the mankind, has become the first cause of the death of world today's population.Estimate according to WHO, cardiovascular and cerebrovascular diseases at least causes the whole world 1,200 ten thousand people dead every year, has become the dead enemy of human health.The high rate of cardiovascular disorder and mortality ratio, guided the direction of medicament research and development and production.In the whole world 10 large well selling medicine in 2005, cardio-cerebralvascular medicine has accounted for 4.Be used for the treatment of cardiovascular Chinese patent medicine and mainly contain three large kinds (the peaceful heart of righting, aromatic herbs activating yang, promoting blood circulation and removing blood stasis).On market, the blood-activating stasis-removing kind drug product situation such as Vitexin is good, increase one of medicine the most rapidly, potentiality are large, the market space wide (Xiong Jiangbo, Sun Liuyan, Wang Ruwei. the Chinese medicine of clinical treatment cardiovascular and cerebrovascular diseases and natural plant [J]. pharmacy monograph, 2009,18 (13): 3-6), containing after Vitexin in discovery Chinese prickly ash, the present invention proposes using Chinese prickly ash plant as the thoughts and methods preparing Vitexin new raw material.
Chinese prickly ash is rutaceae (Zanthoxylumbungeanum), its nature and flavor are recorded pungent, hot, nontoxic in " Chinese Pharmacopoeia ", Folium Zanthoxyli Bungeani can control cold long-pending, cramp caused by acute vomiting and diarrhea, beriberi, dermatitis rhus, scabies, anticorrosion, sterilization, desinsection etc., coldness and pain in the epigastrium can be prevented, the symptoms such as vomiting and diarrhoea, ascariasis, oedema, dysuria.Mainly be distributed in the ground such as Shaanxi, Gansu, Sichuan, Guizhou, Hebei, all can gather the whole year, traditional vegetables of Chinese prickly ash and Ye Shi China and spices, condiment, edible or making green pepper tea can be done.The distribution of China's Chinese prickly ash is wide, wide in variety.Two classes are divided into: hot red pepper, green pepper according to the color and luster of Pericarpium Zanthoxyli fruit; Three classes are divided into: green grass or young crops is fragrant, wormwood artemisia is fragrant, tangerine is fragrant according to the odor type of local flavor; Six classes are roughly divided into: large meatball, Radix Campylotropis Hirtella (Herba Myrsines Africanae), large green pepper (also known as oily green pepper), little green pepper, rice green pepper, beans green pepper according to green pepper shape; Six large regions can be divided into: Yunnan (green pepper), Sichuan (green pepper in Jinyang, the green pepper in Jiangjin, Hanyuan green pepper, luxuriant river in Shangdong Province green pepper), Shaanxi (Radix Campylotropis Hirtella (Herba Myrsines Africanae) of Hancheng, phoenix green pepper), Gansu (volt green pepper, autumn green pepper), Shandong (large green pepper, little green pepper), Hebei (large green pepper, little green pepper) by areal distribution.Wherein, green pepper belongs to the most primary high-grade green pepper, and valency of fine quality is high but yield poorly; Gansu, Shaanxi green pepper belong to middle-grade, and output is large, and suitable batch production scale uses; Shandong, Hebei green pepper soft aroma and, spicy weak, price is low, suitable general consumption (Yu Shengnan. research overview [J] .2012 of Chinese prickly ash, 12 (37): 10-13).Mainly its multiple amide substance contained when Chinese prickly ash uses as seasonings, as: hydroxyl-alpha-sanshool (0.034%), hydroxy-beta-sanshool (0.003%) and hydroxyl-γ-sanshool (0.003%); Amide substance in Chinese prickly ash pericarp has: (the Li Xiaojie such as hydroxyl-alpha-sanshool (2.2%), hydroxy-beta-sanshool (0.01%), hydroxyl-γ-sanshool (5.14%) and phloracetophenone 4,6-dimethyl ether (0.043%), Chen Huaixuan, Xie Wangjun etc. the research [J] of spicy components in pericarpium zanthoxyli bungeani leaf is produced in Hanyuan. Chinese seasonings, 2014,39 (12): 124-128).
Flavonoid substances in xanthoxylum has very strong resistance of oxidation, if Folium Zanthoxyli Bungeani total flavones is 1.8 μ g/mL to the IC50 of DPPH free radical scavenging, be much better than the IC504 μ g/mL of VC, the reducing power of Folium Zanthoxyli Bungeani flavones is significantly better than VC (Xu Huaide, model essence. macroporous adsorbent resin separation and purification Folium Zanthoxyli Bungeani total flavones and products thereof [J]. Food science, 2010,31 (14): 111-115) therefore, Chinese prickly ash is the plant resources being worth exploitation.
Vitexin (C
22h
18o
12) molecular structure is shown below.
Vitexin is one of effective constituent of traditional Chinese medicine, belongs to flavonoid compound.In recent years, the research of Vitexin and Vitexina pharmaceutical use day by day pay close attention to by people, Vitexin and Vitexina have antitumor, anti-oxidant, antiviral, anti-inflammatory, antibacterial, the pharmacological action (Zhang Xue such as step-down and spasmolysis, Xu Daohua. the Advance on Pharmacological Activities [J] of Vitexina. Chinese Medicine Leader, 2013,10 (35): 35-42).Nearest research finds that Vitexin also has neuroprotective; neural damage (LeYang can be repaired; Zhi-mingYang; NanZhang; ZhenTian, Shui-bingLiu, Ming-gaoZhao.Neuroprotectiveeffectsofvitexinbyinhibition ofNMDAreceptorsinprimaryculturesofmousecerebralcorticaln eurons [J] .Mol.Cell.Biochem.; 2014,386:251 – 258).Vitexin is promoting blood circulation and removing blood stasis, regulate the flow of vital energy and promote blood circulation, for the obstruction of qi in the chest caused by hemostasis resistance arteries and veins, disease (the Zhang Xue such as pareordia shouting pain, palpitation and amnesia, dizzy tinnitus, coronary heart disease and angina pectoris, hyperlipidaemia, heart cerebral arterial insufficiency, Xu Daohua. the Advance on Pharmacological Activities [J] of Vitexina. Chinese Medicine Leader, 2013,10 (35): 35-42).
Produce the method for Vitexin to mainly contain chemosynthesis and extract two kinds of methods from plant, chemosynthesis is higher and the security etc. of raw material makes people have doubt to product to equipment requirements.Because the Vitex negundo var cannabifolia growth of wild state is scattered, large-scale planting Vitex negundo var cannabifolia amount is few, and in the Vitex negundo var cannabifolia that conventional Chinese medicinal materials is gathered, the content of Vitexin is low and make the under-supply of Vitexin.Normally used alternative materials hawthorn (Shao Xu etc., the extracting method of Vitexin, purposes and preparation [P], CN1683386) its fruit or leaf in Vitexin content (0.06mg/g) also not high (Zhao Caiyun, Wang Qiang .HPLC measures Vitexin-4 "-O-glucoside in Howthorn Leaf, vitexin rhamnoside, Vitexin, rutin, the content [J] of Quercetin 3-galactoside. Chinese patent medicine, 2011, 33 (3): 494-497), therefore the Chinese prickly ash raw material that the present invention improves all has huge advantage from biomass or from aspects such as Vitexin content (13.23mg/g).
Summary of the invention
The invention provides a kind of Chinese prickly ash plant is the method that Vitexin prepared by raw material, provides a kind of new approach for producing Vitexin.
The technical solution adopted in the present invention is:
Each position of Chinese prickly ash plant, as the application preparing Vitexin raw material, comprises various wild Chinese prickly ash and the plantation Chinese prickly ash of Zanthoxylum.
Described raw material is Folium Zanthoxyli Bungeani, or for extracting the Chinese prickly ash residue after Japan pepper essential oil.Also can be Chinese prickly ash fruit, pericarp stem or root skin, or they extract the residue after Japan pepper essential oil.
Present invention also offers a kind of Chinese prickly ash plant is the method that Vitexin prepared by raw material, be by each position of Chinese prickly ash plant be raw material through Extraction solvent lixiviate, obtain the extracting solution containing Vitexin, then obtain Vitexin through separation and purification.
Described Extraction solvent is the mixed solution of the single components such as water, methyl alcohol, ethanol, acetone, ethyl acetate, propyl carbinol, Virahol, lipid, ketone or two or more different ratioss.
Described extracting mode is the combination of one or more forms in leaching extraction, ultrasound assisted extraction, boiling, microwave radiation exaraction, counter-current extraction.
The pH of described Extraction solvent is 1.0-9.0, the solid-liquid ratio 1:5-100 (w/w) of raw material and Extraction solvent, extraction time 0.1-20h.
Pericarpium Zanthoxyli extract, through solvent extraction, macroporous resin adsorption, polyamide chromatography, silica gel column chromatography, preparative liquid chromatography preparation or high performance countercurrent chromatography method fractionation by adsorption, obtains Vitexin finished product after refining.
Macroporous resin used is nonpolar or polar macroporous resin, and be preferably extremely non-property post, resin can be one or more in D101, D301, HPD100, HPD300, X-5, XAD-2, but is not limited in above listed kind.
Solvent used is polarity or non-polar solvent, is preferably non-polar solvent, can be one or more in ethyl acetate, normal hexane etc., but be not limited in above listed kind.
Research of the present invention finds, Vitexin content in Chinese prickly ash is also very high, far above the content (Zhao Caiyun in Vitexin traditional mode of production raw material Howthorn Leaf (0.05mg/g), Vitex negundo var cannabifolia (3.23mg/g), paper mulberry (0.4mg/g) etc., Wang Qiang .HPLC measures Vitexin-4 in Howthorn Leaf " content [J] of-O-glucoside, vitexin rhamnoside, Vitexin, rutin, Quercetin 3-galactoside. Chinese patent medicine, 2011,33 (30): 494-497, YujuanZhang, DongmeiWang, LinaYang, DanZhou, JingfangZhang.PurificationandCharacterizationofFlavonoid sfromtheLeavesofZanthoxylumbungeanumandCorrelationbetwee nTheirStructureandAntioxidantActivity [J] .PLOSONE, 2014, 9 (8): 1-11). therefore, the qualification to Vitexin in Chinese prickly ash that the present invention proposes, Extraction and separation, the preparation of Chinese prickly ash novelty teabag and Vitexin is had important practical significance, for comprehensive utilization Folium Zanthoxyli Bungeani, really, the raw materials such as skin provide novel method, Pericarpium Zanthoxyli extract HPLC analyzes as shown in Figure 1.Feed with paper-mulberry leaf extracting solution HPLC analyzes as shown in Figure 2, and Vitexin qualitative and quantitative is wherein by carrying out with comparing of mark product Vitexin (see Fig. 3 and Fig. 4).
Accompanying drawing explanation
The HPLC figure of Fig. 1 Folium Zanthoxyli Bungeani extracting solution.
The HPLC figure of Fig. 2 feed with paper-mulberry leaf extracting solution.
Fig. 3 Vitexin mark product HPLC schemes.
Fig. 4 Vitexin second order ms figure.
Embodiment
Further illustrate the present invention below in conjunction with embodiment, citing is for process of the present invention being described but not being only limitted to embodiment.
Embodiment 1
Pericarpium zanthoxyli schinifolii leaf raw material 1000g, the ethanolic soln of volumetric concentration 60% is added by solid-to-liquid ratio 1:10 (w/w), lixiviate 60min under ultrasonication, the extracting solution containing Vitexin is obtained through solid-liquid separation, recycling design obtains medicinal extract 30g, is separated obtains the Vitexin 11.3g that purity is 90% through polyamide column.
Embodiment 2
Chinese prickly ash pericarp raw material 1000g, add water, heating and refluxing extraction 60min by solid-to-liquid ratio 1:50 (w/w), after solid-liquid separation, obtain extracting solution, vaporize water obtains medicinal extract 35g, and medicinal extract obtains through D101 resin isolation the Vitexin 9.1g that purity is 95%.
Embodiment 3
Pericarpium zanthoxyli bungeani fruit raw material 1000g, the methanol solution of 50% is added by solid-to-liquid ratio 1:30 (w/w), lixiviate 120min under microwave-assisted effect, after solid-liquid separation, obtain the extracting solution containing Vitexin, recycling design obtains medicinal extract 28g, is separated obtains the Vitexin 10.2g that purity is 92% through silica gel column chromatography.
Embodiment 4
Pericarpium zanthoxyli schinifolii leaf raw material 1000g, adds the methanol solution of pH6.0 volumetric concentration 70% by solid-to-liquid ratio 1:20 (w/w), lixiviate 30min under ultrasonication, and extracting solution, through high performance countercurrent chromatography separation and purification, obtains the Vitexin 11.8g that purity is 98%.
Embodiment 5
Radix Zanthoxyli Bungeani 1000g, add the ethyl acetate solution of the volumetric concentration 50% of pH5.0 by solid-to-liquid ratio 1:10 (w/w) after pulverizing, lixiviate 80min under ultrasonication, after solid-liquid separation, obtain the extracting solution containing Vitexin, after recycling design, be separated through polymeric amide and obtain the Vitexin 8.9g that purity is 90%.
Embodiment 6
Leaflet Folium Zanthoxyli Bungeani 1000g, add the ethyl acetate solution of volumetric concentration 60% by solid-to-liquid ratio 1:10 (w/w) after pulverizing, lixiviate 60min under ultrasonication, the extracting solution containing Vitexin is obtained through solid-liquid separation, after receiving solvent, obtain through column chromatography the Vitexin 10.4g that purity is 95%.
Embodiment 7
Zanthoxylum armatum leaf 1000g, add the ethanolic soln of volumetric concentration 60% by solid-to-liquid ratio 1:10 (w/w) after pulverizing, lixiviate 60min under microwave action, after solid-liquid separation, obtain the extracting solution containing Vitexin, after recycling design, obtain through methyl aceto acetate solvent extraction and separation the Vitexin 10.7g that purity is 91%.
Embodiment 8
Folium Zanthoxyli Bungeani raw material 1000g, add the methyl ethyl ketone solution of volumetric concentration 60% by solid-to-liquid ratio 1:10 (w/w) after pulverizing, lixiviate 60min under ultrasonication, after solid-liquid separation, obtain the extracting solution containing Vitexin, recycling design obtains medicinal extract 27g, obtains the Vitexin 11.4g that purity is 90% after polymeric amide is separated.
Embodiment 9
Identical with embodiment 8, Extraction solvent is changed to propyl carbinol.Extracting solution D101 macroporous resin, through conventional fractionation by adsorption with after refining, obtains the Vitexin and chlorogenic acid, the quinic acid that contain 90% respectively.
Embodiment 10
Identical with embodiment 8, Extraction solvent is changed to Virahol.
Embodiment 11
Take the Folium Zanthoxyli Bungeani residuum 100g after Petroleum ether extraction Japan pepper essential oil, add the butanol solution of 60% by solid-liquid ratio 1:50 (w/v), 45 DEG C of supersound extraction 70min, separation and purification is with embodiment 7.
Embodiment 12
Identical with embodiment 11, change D101 macroporous resin into D301 macroporous resin.
Embodiment 13
Identical with embodiment 11, change D101 macroporous resin into HPD300 macroporous resin.
Embodiment 14
Identical with embodiment 11, change D101 macroporous resin into HPD100 macroporous resin.
Embodiment 15
Identical with embodiment 11, change D101 macroporous resin into XAD-2 macroporous resin.
Embodiment 16
Identical with embodiment 5, change pH into 9.0.
Embodiment 17
Identical with embodiment 5, change pH into 1.0.
Embodiment 18
Identical with embodiment 5, solid-liquid ratio is changed into 1:5 (w/w).
Embodiment 19
Identical with embodiment 6, solid-liquid ratio is changed into 1:100 (w/w).
Embodiment 20
Identical with embodiment 5, change extraction time into 20h.
Embodiment 21
Identical with embodiment 5, change extraction time into 0.1h.
Claims (10)
1. each position of Chinese prickly ash plant is as the application preparing Vitexin raw material.
2. apply as claimed in claim 1, it is characterized in that described raw material is Folium Zanthoxyli Bungeani, or for extracting the Chinese prickly ash residue after Japan pepper essential oil.
3. apply as claimed in claim 1, it is characterized in that described raw material is Chinese prickly ash fruit, pericarp stem or root skin, or they extract the residue after Japan pepper essential oil.
4. be the method that Vitexin prepared by raw material with Chinese prickly ash plant, be by each position of Chinese prickly ash plant be raw material through Extraction solvent lixiviate, obtain the extracting solution containing Vitexin, then obtain Vitexin through separation and purification.
5. method according to claim 4, is characterized in that described Extraction solvent is the mixed solution of the single components such as water, methyl alcohol, ethanol, acetone, ethyl acetate, propyl carbinol, Virahol, lipid, ketone or two or more different ratioss.
6. method according to claim 4, is characterized in that described extracting mode is the combination of one or more forms in leaching extraction, ultrasound assisted extraction, boiling, microwave radiation exaraction, counter-current extraction.
7. method according to claim 4, is characterized in that the pH of described Extraction solvent is 1.0-9.0, the solid-liquid ratio 1:5-100 (w/w) of raw material and Extraction solvent, extraction time 0.1-20h.
8. method according to claim 4, is characterized in that Pericarpium Zanthoxyli extract is through solvent extraction, macroporous resin adsorption, polyamide chromatography, silica gel column chromatography, preparative liquid chromatography preparation or high performance countercurrent chromatography method fractionation by adsorption, obtains Vitexin finished product after refining.
9. method as claimed in claim 8, it is characterized in that macroporous resin used is for being extremely non-property post, resin is D101, D301, HPD100, HPD300, X-5, one or more in XAD-2.
10. method as claimed in claim 7, is characterized in that solvent used is non-polar solvent.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050078886A (en) * | 2004-02-03 | 2005-08-08 | 엘지전자 주식회사 | Control system of air-cleaner and the method for the same |
| CN1935150A (en) * | 2006-06-22 | 2007-03-28 | 合肥七星医药科技有限公司 | Pharmaceutical use of vitexin for anti-myocardial ischemia action |
| CN101508711A (en) * | 2009-03-26 | 2009-08-19 | 浙江林学院 | Method for separating and purifying flavonoid glycoside monomer from sensitive plant |
| CN102040593A (en) * | 2010-09-27 | 2011-05-04 | 南京泽朗医药科技有限公司 | Method for simultaneously extracting vitexin and quercetin from Chinese hawthorn leaf |
-
2015
- 2015-10-16 CN CN201510670560.2A patent/CN105237522A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050078886A (en) * | 2004-02-03 | 2005-08-08 | 엘지전자 주식회사 | Control system of air-cleaner and the method for the same |
| CN1935150A (en) * | 2006-06-22 | 2007-03-28 | 合肥七星医药科技有限公司 | Pharmaceutical use of vitexin for anti-myocardial ischemia action |
| CN101508711A (en) * | 2009-03-26 | 2009-08-19 | 浙江林学院 | Method for separating and purifying flavonoid glycoside monomer from sensitive plant |
| CN102040593A (en) * | 2010-09-27 | 2011-05-04 | 南京泽朗医药科技有限公司 | Method for simultaneously extracting vitexin and quercetin from Chinese hawthorn leaf |
Non-Patent Citations (3)
| Title |
|---|
| 张玉娟: "花椒叶抗氧化活性成分的分离、结构鉴定及其构效关系", 《西北农林科技大学2014届攻读硕士学位研究生学位论文》 * |
| 杨立琛: "花椒叶黄酮的微波提取及其成分分析", 《天津商业大学硕士学位论文》 * |
| 赵湘湘，等: "黄荆子的化学成分研究", 《中草药》 * |
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Application publication date: 20160113 |