CN105232611A - Microwave wall-breaking extraction process of gynostemma pentaphylla extracting solution - Google Patents
Microwave wall-breaking extraction process of gynostemma pentaphylla extracting solution Download PDFInfo
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- CN105232611A CN105232611A CN201510680964.XA CN201510680964A CN105232611A CN 105232611 A CN105232611 A CN 105232611A CN 201510680964 A CN201510680964 A CN 201510680964A CN 105232611 A CN105232611 A CN 105232611A
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Abstract
The invention discloses a microwave wall-breaking extraction process of a gynostemma pentaphylla extracting solution, and discloses a technological method for preparing a gynostemma pentaphylla extracting solution through wall breaking by combination of microwave and enzymic methods. The method comprises the following steps: 1, gynostemma pentaphylla smashing; 2, microwave wall-breaking; 3, enzymolysis extraction; 4, heating up and enzyme deactivation; and 5, refrigerating and filtering. The method can significantly improve the extraction rate of total saponins in the gynostemma pentaphylla extracting solution and shorten the extraction time, and the gynostemma pentaphylla extracting solution production process is applicable to large-scale industrial production.
Description
Technical field
The present invention relates to technical field of extraction of Chinese traditional medicine, be specifically related to the process that a kind of microwave and enzyme method combined wall breakage obtain Herb Gynostemmae Pentaphylli extract.
Background technology
Herb Gynostemmae Pentaphylli, also known as: paradise grass, Gospel are careless, Chao Rencan, Herb Gynostemmae Pentaphylli, Herba Gynostemmatis, Herba Gynostemmatis, Pentapanax and gynostemma pentaphyllum makino etc., and Japan is referred to as sweet climing tea; Its stem is thin and delicate, tool branch, and tool indulges rib and groove, without hair or pubescence.Mainly be distributed in the provinces such as Shaanxi flat interest, Hunan, Yunnan, Guangxi in China, grow at the medicinal comparision contents of southern Herb Gynostemmae Pentaphylli high, be known as " southern Radix Ginseng ".1986, State Scientific and Technological Commission, in Spark Program, was classified as Herb Gynostemmae Pentaphylli the first place of " rare Chinese medicine " leaved for development, and on March 5th, 2002, health ministry was listed in health product list.
Herb Gynostemmae Pentaphylli has blood fat reducing, blood pressure control thrombosis, control cardiovascular disease, regulates blood glucose, short sleep, slow old and feeble, anti-anticancer, the multi-efficiency that improves immunity, regulate human physiological functions.And Herb Gynostemmae Pentaphylli can protect adrenal gland and thymus and the endocrine organ unlikely atrophy with the growth at age, maintain the function of hormonal system, and there is blood sugar lowering and improve carbohydrate metabolism.
Because Herb Gynostemmae Pentaphylli has higher health value, recent year has done many research in the preparation of Herb Gynostemmae Pentaphylli extract, but be extract take acidity or alkali condition on the basis that traditional water bath extracts under mostly, or employing ethanol extraction, the pH changing extracting solution after concentrated extracts further, although technique is simple, extraction effect is unsatisfactory, and extraction time is longer.And relatively the ethanol extraction of the more effective ingredient of acquisition can consume a large amount of ethanol, production cost increases and the later stage reclaims loaded down with trivial details, easily has dissolvent residual, and this just causes part waste of Herb Gynostemmae Pentaphylli resource, can not play its due effect.
Summary of the invention
It is simple that technical problem to be solved by this invention is to provide a kind of method, the microwave frequency measurment extraction process of the Herb Gynostemmae Pentaphylli extract that extraction efficiency is high.
Technical problem to be solved by this invention realizes by the following technical solutions:
A microwave frequency measurment extraction process for Herb Gynostemmae Pentaphylli extract, specifically comprises the following steps:
(1) Herb Gynostemmae Pentaphylli is pulverized: Herb Gynostemmae Pentaphylli powder is broken to 40 ~ 80 orders, obtains Herb Gynostemmae Pentaphylli powder stand-by;
(2) microwave frequency measurment: regulate Herb Gynostemmae Pentaphylli powder humidity to be 10 ~ 60%, batch microwave process, makes final temperature not exceed 100 DEG C;
(3) enzymolysis and extraction: the Herb Gynostemmae Pentaphylli after microwave frequency measurment is heated to 40 ~ 60 DEG C in the acid medium of pH=4 ~ 6, adds solid enzyme and stirs and maintain the temperature at 40 ~ 60 DEG C of lixiviate 1 ~ 4h, obtain extracting solution stand-by;
(4) intensification enzyme denaturing: to raise extracting solution temperature to 70 ~ 80 DEG C and keep 1 ~ 4h;
(5) cold preservation is filtered: by cold preservation 12-36h after extracting solution cooling, obtain Herb Gynostemmae Pentaphylli extract after filtering.
Step (2) discontinuous microwave treatment 30 ~ 120s, time out is determined according to Herb Gynostemmae Pentaphylli powder temperature, just can continue microwave treatment lower than 100 DEG C.
In step (2), microwave frequency measurment batch process number of times is 4 ~ 10 times.
Acid medium is the aqueous solution adopting Fructus Citri Limoniae acid for adjusting pH to 4 ~ 6 in step (3).
Enzyme described in step (3) is one or both or three kinds of combinations in cellulase, hemicellulase, xylanase, and the addition of single enzyme is that every kilogram of Herb Gynostemmae Pentaphylli powder adds 1 ~ 6g, and the enzyme of three kinds of enzymes is lived and is 50,000 U/g.
In step (3), adding of solid enzyme is that substep adds a kind of enzyme or adds two or three compound enzyme simultaneously.
The present invention compared with prior art at least has following characteristics:
1. in the extracting solution made by this technique, saponin content is apparently higher than traditional water extraction or alcohol extraction.
2. extraction time of the present invention is short, substantially increases extraction efficiency.
3. present invention process operating unit is distinct, and equipment needed thereby is simple, is applicable to suitability for industrialized production.
Detailed description of the invention
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.
Embodiment 1: Herb Gynostemmae Pentaphylli powder is broken to 60 orders, regulate Herb Gynostemmae Pentaphylli powder humidity to be 20%, uninterrupted microwave process, processes 30s at every turn, process 4 times, by the Herb Gynostemmae Pentaphylli after microwave frequency measurment in the heated in water solution of pH=4 to 50 DEG C, add cellulase and stir, the proportion of enzyme and Herb Gynostemmae Pentaphylli powder is 1:200, and maintain the temperature at 50 DEG C of lixiviate 1h, raising extracting solution temperature to 70 DEG C keeps 1h, cold preservation 12h after cooling, after filtering Herb Gynostemmae Pentaphylli extract.
Embodiment 2: Herb Gynostemmae Pentaphylli powder is broken to 60 orders, regulate Herb Gynostemmae Pentaphylli powder humidity to be 30%, uninterrupted microwave process, processes 30s at every turn, process 4 times, by the Herb Gynostemmae Pentaphylli after microwave frequency measurment in the heated in water solution of pH=4 to 50 DEG C, add cellulase and hemicellulase stirs, the proportion of two kinds of enzymes and Herb Gynostemmae Pentaphylli powder is as 1:400, and maintain the temperature at 50 DEG C of lixiviate 1h, raising extracting solution temperature to 70 DEG C keeps 1h, cold preservation 12h after cooling, after filtering Herb Gynostemmae Pentaphylli extract.
Embodiment 3: Herb Gynostemmae Pentaphylli powder is broken to 80 orders, regulate Herb Gynostemmae Pentaphylli powder humidity to be 40%, uninterrupted microwave process, processes 30s at every turn, process 6 times, by the Herb Gynostemmae Pentaphylli after microwave frequency measurment in the heated in water solution of pH=4 to 45 DEG C, add cellulase and hemicellulase stirs, the proportion of two kinds of enzymes and Herb Gynostemmae Pentaphylli powder is as 1:400, and maintain the temperature at 50 DEG C of lixiviate 1h, raising extracting solution temperature to 70 DEG C keeps 1h, cold preservation 12h after cooling, after filtering Herb Gynostemmae Pentaphylli extract.
Embodiment 4: Herb Gynostemmae Pentaphylli powder is broken to 80 orders, Herb Gynostemmae Pentaphylli powder humidity is regulated to be 30%, uninterrupted microwave process, each process 60s, process 4 times, by the Herb Gynostemmae Pentaphylli after microwave frequency measurment in the heated in water solution of pH=4 to 50 DEG C, add cellulase, hemicellulase and xylanase to stir, the proportion of three kinds of enzymes and Herb Gynostemmae Pentaphylli powder is as 1:500, and maintain the temperature at 45 DEG C of lixiviate 1h, raising extracting solution temperature to 70 DEG C keeps 1h, cold preservation 12h after cooling, after filtering Herb Gynostemmae Pentaphylli extract.
The above-mentioned technique of the present invention and existing decocting method, alcohol extraction are followed the example of extraction and be the results are shown in following table, and result shows that shell-broken effect of the present invention is good, and gypenoside extraction ratio is high.
More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and description just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (7)
1. the microwave frequency measurment extraction process of a Herb Gynostemmae Pentaphylli extract, it is characterized in that: after being pulverized by Herb Gynostemmae Pentaphylli, microwave is interrupted broken wall treatment, and after ensureing process, temperature is no more than 100 DEG C, then carries out enzymolysis and extraction, carry out intensification enzyme denaturing after extraction, cold preservation is filtered and is obtained Herb Gynostemmae Pentaphylli extract.
2. the microwave frequency measurment extraction process of a kind of Herb Gynostemmae Pentaphylli extract according to claim 1, is characterized in that concrete steps are as follows:
(1) Herb Gynostemmae Pentaphylli is pulverized: Herb Gynostemmae Pentaphylli powder is broken to 40 ~ 80 orders, obtains Herb Gynostemmae Pentaphylli powder stand-by;
(2) microwave frequency measurment: regulate Herb Gynostemmae Pentaphylli powder humidity to be 10 ~ 60%, uninterrupted microwave process, makes final temperature not exceed 100 DEG C;
(3) enzymolysis and extraction: the Herb Gynostemmae Pentaphylli after microwave frequency measurment is heated to 40 ~ 60 DEG C in the acid medium of pH=4 ~ 6, adds solid enzyme and stirs and maintain the temperature at 40 ~ 60 DEG C of lixiviate 1 ~ 4h, obtain extracting solution stand-by;
(4) intensification enzyme denaturing: to raise extracting solution temperature to 70 ~ 80 DEG C and keep 1 ~ 4h;
(5) cold preservation is filtered: by cold preservation 12-36h after extracting solution cooling, obtain Herb Gynostemmae Pentaphylli extract after filtering.
3. technique according to claim 2, it is characterized in that being interrupted microwave treatment 30 ~ 120s in step (2), time out is determined according to Herb Gynostemmae Pentaphylli powder temperature, just continues microwave treatment lower than 100 DEG C.
4. technique according to claim 2, is characterized in that in step (2), microwave frequency measurment intermittent processing number of times is 4 ~ 10 times.
5. technique according to claim 2, is characterized in that enzyme described in step (3) is one or both or three kinds of combinations in cellulase, hemicellulase, xylanase.
6. technique according to claim 2, it is characterized in that the addition of enzyme described in step (3) is that every kilogram of Herb Gynostemmae Pentaphylli powder adds 1 ~ 6g, the enzyme of three kinds of enzymes is lived and is 50,000 U/g.
7. technique according to claim 2, is characterized in that in step (3), adding of enzyme is that substep adds a kind of enzyme or adds two or three compound enzyme simultaneously.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105664681A (en) * | 2016-03-02 | 2016-06-15 | 许五妮 | High-efficiency extraction process of onion extract liquid |
| CN105747101A (en) * | 2016-03-01 | 2016-07-13 | 田金举 | Nutritional noodle with rich wheat and oligopeptide-selenium gynostemma pentaphylla and processing process |
| CN105747105A (en) * | 2016-03-01 | 2016-07-13 | 田金举 | Nutrient noodle rich in wheat, oligopeptide, selenium and rose and making process |
| CN105767885A (en) * | 2016-03-01 | 2016-07-20 | 田金举 | Wheat oligopeptide-rich selenium panax notoginseng nutritive noodle and machining process thereof |
| CN105816709A (en) * | 2016-04-11 | 2016-08-03 | 安徽仁之堂药业有限公司 | High efficient preparation method of traditional Chinese medicine massa medicata fermentata formula granules |
| CN105936762A (en) * | 2016-06-06 | 2016-09-14 | 曹美兰 | Aromatic environment-friendly formaldehyde eliminating paint additive |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103113975A (en) * | 2013-02-05 | 2013-05-22 | 中国林业科学研究院林产化学工业研究所 | Method for extracting tea oil and saccharicterpenin by using physical wall breaking to assist aqueous enzymatic method |
-
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103113975A (en) * | 2013-02-05 | 2013-05-22 | 中国林业科学研究院林产化学工业研究所 | Method for extracting tea oil and saccharicterpenin by using physical wall breaking to assist aqueous enzymatic method |
Non-Patent Citations (2)
| Title |
|---|
| 张新宇等: "绞股蓝皂苷提取纯化工艺研究进展", 《食品工业科技》 * |
| 黄山等: "酶法提取绞股蓝总皂苷工艺条件的优化", 《食品工业科技》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105747101A (en) * | 2016-03-01 | 2016-07-13 | 田金举 | Nutritional noodle with rich wheat and oligopeptide-selenium gynostemma pentaphylla and processing process |
| CN105747105A (en) * | 2016-03-01 | 2016-07-13 | 田金举 | Nutrient noodle rich in wheat, oligopeptide, selenium and rose and making process |
| CN105767885A (en) * | 2016-03-01 | 2016-07-20 | 田金举 | Wheat oligopeptide-rich selenium panax notoginseng nutritive noodle and machining process thereof |
| CN105664681A (en) * | 2016-03-02 | 2016-06-15 | 许五妮 | High-efficiency extraction process of onion extract liquid |
| CN105816709A (en) * | 2016-04-11 | 2016-08-03 | 安徽仁之堂药业有限公司 | High efficient preparation method of traditional Chinese medicine massa medicata fermentata formula granules |
| CN105936762A (en) * | 2016-06-06 | 2016-09-14 | 曹美兰 | Aromatic environment-friendly formaldehyde eliminating paint additive |
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Address after: 99 No. 236500 Anhui city of Fuyang Province Jieshou City feigexin Avenue Applicant after: ANHUI FINDSHE BIOLOGICAL PHARMACEUTICAL CO., LTD. Address before: 99 No. 236500 Anhui city of Fuyang Province Jieshou City feigexin Avenue Applicant before: Anhui Funland Biological Pharmaceutical Co., Ltd. |
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