CN105218265B - A kind of micro organism composite fertilizer for preventing jute root knot nematode disease - Google Patents
A kind of micro organism composite fertilizer for preventing jute root knot nematode disease Download PDFInfo
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- CN105218265B CN105218265B CN201510630017.XA CN201510630017A CN105218265B CN 105218265 B CN105218265 B CN 105218265B CN 201510630017 A CN201510630017 A CN 201510630017A CN 105218265 B CN105218265 B CN 105218265B
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Abstract
The present invention relates to microbial manures, specifically provide a kind of micro organism composite fertilizer for preventing jute root knot nematode disease, and the micro organism composite fertilizer is made of the raw material of following parts by weight：6~10 parts of Bacillus cereus microbial inoculum after 15~25 times of dilution, 990~1000 parts of organic fertilizer, 100~150 parts of inorganic fertilizer；The viable bacteria concentration of Bacillus cereus is not less than 10 in the microbial inoculum9cfu/ml.Micro organism composite fertilizer of the present invention can effectively inhibit the hatching of root-knot nematode egg and root-knot nematode to infect jute root system, and can guarantee the nutriment needed for jute growth, and long-time service can improve soil quality.
Description
Technical field
The present invention relates to microbial manure, specifically, it is compound to be related to a kind of microorganism for preventing jute root knot nematode disease
Fertilizer.
Background technology
Jute is the annual bast fiber crop of Tiliaceae (Tiliaceae) Corchorus (Corchorus), fiber finer
It is soft, strength is big, good hygroscopicity, aproll are fast, good spinnability, textile raw material, the raw material of industry and biomass energy etc., purposes can be used as
It is very wide.
Root knot nematode disease occurs seriously, to influence the yield and quality of jute big in each numb area.Jute root-knot nematode is in seedling
Phase, Adult plant can all occur.Root is come into being many tiny root nodules, after can grow to mung bean to soybean or broad bean grain size.At the beginning of insect gall
For yellow-white, rear browning or full root rot.Raw dozens of root nodule on every plant of root system when serious, some mutually fusions cause full root or
Lateral root swelling torsional deformation, radicula hair is seldom, and overground part leaf color turns yellow or strain is withered.
For the control measure of jute root knot nematode disease, following measures are usually taken at present：(1) crop rotation：Rice, sisal work are
Prevent this comparatively ideal method of disease.If carry out rice without condition, sisal is made, crop rotation can be carried out with cotton or coarse cereals etc..(2) it ploughs deeply
Soil：25 centimetres or more are turned over sickly, and the more topsoil of worm sources is translated into deep layer, can mitigate morbidity.(3) fertilising and filling
It irrigates：Organic base manure is increased, imposes nitrogen, phosphorus, potash fertilizer, arid season is irrigated in time, makes numb strain robust growth, enhances premunition.(4)
Chemical control：Broadcast preceding 2.5~4 kilograms of 3~4 kilograms of 3% carbofuran Granules of ditch spread or 5% Aldicarb granule per acre or drop
30~40 kilograms of D-D soil fumigant has certain disease-preventing and yield-increasing to act on.
Above-mentioned means have certain effect to the prevention of jute root knot nematode disease, but effect is not notable, and are easy to cause
Human cost increases, although chemical insecticide preventive effect is notable, the growing environment of destruction crops, and not degradable, high residue,
Soil texture is destroyed, the drug resistance problems of nematode also become increasingly conspicuous.
Invention content
In order to solve the problems in the existing technology, the object of the present invention is to provide a kind of prevention jute root knot nematode diseases
Micro organism composite fertilizer.
In order to realize the object of the invention, present invention firstly provides a kind of microorganism for preventing jute root knot nematode disease is compound
Fertilizer, the micro organism composite fertilizer are made of the raw material of following parts by weight：Dilution 15~25 times after Bacillus cereus microbial inoculum 6~
10 parts, 990~1000 parts of organic fertilizer, 100~150 parts of inorganic fertilizer；The viable bacteria concentration of Bacillus cereus is not small in the microbial inoculum
In 109cfu/ml。
Preferably, the micro organism composite fertilizer is made of the raw material of following parts by weight：Waxy brood cell's bar of 20 times of dilution
6~10 parts of bacteria agent, 990~1000 parts of organic fertilizer, 100~150 parts of inorganic fertilizer.
More preferably, the micro organism composite fertilizer is made of the raw material of following parts by weight：Waxy brood cell's bar of 20 times of dilution
8~10 parts of bacteria agent, 990~995 parts of organic fertilizer, 125~150 parts of inorganic fertilizer.
Further, the preparation method of the Bacillus cereus microbial inoculum includes the following steps：
Bacillus cercus strain is seeded on PDA plate, through 3 activation, with the single bacterium on transfer needle picking tablet
Into the test tube containing PDA culture solutions, kind of a bacterium is made in culture for 24 hours；By 1：Kind of a bacterium is connected to containing PDA culture solutions by 100 ratio
Triangular flask in continue culture for 24 hours be made microbial inoculum.
Preferably, said waxy Bacillus is Bacillus cercus AT31, it is preserved on June 18th, 2009
China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number are CGMCC NO.3128.
The present invention also provides application of the micro organism composite fertilizer in jute root knot nematode disease is prevented.
Further, the micro organism composite fertilizer can be used as base manure or top dressing to use.
When the micro organism composite fertilizer is as base manure, preferably, using 20~80kg per acre.
When the micro organism composite fertilizer is as top dressing, apply preferably, shifting to an earlier date 7~10 days than chemical fertilizer, per acre using 10
~50kg.
The micro organism composite fertilizer may further be used to the seed dressing before being sowed.
The beneficial effects of the present invention are：
Micro organism composite fertilizer provided by the invention can effectively inhibit root-knot nematode egg hatching and root-knot nematode to roundpod jute root
System infects, and can guarantee the nutriment needed for jute growth, and long-time service can improve soil quality.
Description of the drawings
Fig. 1 is the method flow diagram that the embodiment of the present invention 7 prevents jute root knot nematode disease.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
The preparation of 1 microbial inoculum of embodiment
Bacillus cercus AT31 strains are seeded on PDA (potato dextrose agar) tablet, through 3 work
Change, in the single bacterium to the test tube containing PDA culture solutions on transfer needle picking tablet, kind of a bacterium is made in left and right for 24 hours for culture；By 1：
Kind of bacterium is connected in the triangular flask containing PDA culture solutions by 100 ratio continues the obtained microbial inoculum of culture 24 hours or so, in the microbial inoculum
Viable bacteria concentration is not less than 109cfu/ml。
Said waxy bacillus AT31 is Bacillus cercus (Bacillus cereus), is protected on June 18th, 2009
China Committee for Culture Collection of Microorganisms's common micro-organisms center is hidden in, culture presevation number is CGMCC NO.3128.
The preparation of 2 micro organism composite fertilizer of embodiment
(1) preparation of organic fertilizer：By compost, make compost, cake fertilizer and jute stalk are according to weight ratio 0.6:0.8:1 mixing, adds
Enter suitable water, adjustment water content is 30%~50%, obtains organic fertilizer, wherein the compost is roundpod jute leaf and tender slightly by arbitrary
Naturally decomposed fermentation obtains after ratio mixing, makes compost as jute blade, tender obtained under flooding condition slightly with stalk, cake fertilizer is dish
Seedcake.
(2) preparation of inorganic fertilizer：By ammonium sulfate, ammonium dihydrogen phosphate and calcium magnesium phosphate according to weight ratio 2:3:2 ratio is mixed
It closes, and is dissolved in water.
(3) the Bacillus cercus microbial inoculum for preparing embodiment 1 dilutes 20 times, adds in the organic fertilizer that step (1) obtains,
It adds in amount of water to stir evenly, is eventually adding the inorganic fertilizer of step (2) acquisition, be again stirring for uniformly, wherein the bacterium after dilution
6 parts of agent, 1000 parts of organic fertilizer, 100 parts of inorganic fertilizer.
(4) fertilizer stirred evenly is carried out low temperature (temperature be less than 40 DEG C) to air-dry, control moisture for 35% to get
Micro organism composite fertilizer.
Embodiment 3~6
Embodiment 3~6 and embodiment 1 difference lies in：Microbial bacterial agent after dilution and organic fertilizer and inorganic fertilizer are matched
Than as follows：
| Microbial bacterial agent after dilution | Organic fertilizer | Inorganic fertilizer | |
| Embodiment 3 | 7 | 1000 | 100 |
| Embodiment 4 | 8 | 995 | 125 |
| Embodiment 5 | 9 | 990 | 150 |
| Embodiment 6 | 10 | 990 | 150 |
The method that embodiment 7 prevents jute root knot nematode disease
The present embodiment provides the methods (as shown in Figure 1) of different phase prevention jute root knot nematode disease grown in jute.
First, before sowing
(1) it dresses seed：Micro organism composite fertilizer prepared by embodiment 2 is thoroughly mixed with jute seed, make seed with it is micro-
Biological compound fertilizer is sowed after coming into full contact with；
(2) it soaks seed：Microbial inoculum prepared by embodiment 1 dilutes 10 times, impregnates seed, pulls out and dry, sowed when seed shows money or valuables one carries unintentionally.
2nd, Seedling Stage
(1) root dipping：Microbial inoculum prepared by embodiment 1 dilutes 15 times, is stood after liquid adhesional wetting is immersed in root before seedling replanting
Take out；
(2) root is sprayed：When there are many seedling, microbial inoculum prepared by embodiment 1 dilutes 15 times, is put into spray canister and squirts root i.e.
It can.
3rd, growth period
Micro organism composite fertilizer prepared by embodiment 2, for the nutritional supplement in jute growth period.
(1) make base manure：It is applied together with farm manure during site preparation, per acre using 20~80kg；
(2) make top dressing：7~10 days should be shifted to an earlier date than chemical fertilizer when doing top dressing with biological organic fertilizer, per acre using 10~50kg.
Experimental example 1 prevents the optimization of jute root knot nematode disease method
It is right after the Bacillus cercus microbial inoculum prepared by pot experiment detection embodiment 1 carries out different degrees of dilution
The control effect of jute root knot nematode disease.
Detection experiment is divided into two parts：The root dipping effect of effect of soaking seed, Seedling Stage before sowing.
Comparison of the jute seed with compareing root-knot nematode resistant effect after the seed soaking of 1 microbial inoculum of table
By table 1 as it can be seen that performance of the jute seed in terms of resisting root-knot nematode and infecting after microbial inoculum seed soaking is bright
When showing and be better than control, and the microbial inoculum for diluting 10 times inhibits the better of root-knot nematode, therefore seed is impregnated using microbial inoculum, preferably
Extension rate is 10 times.
Comparison of the jute seedling of 2 microbial inoculum root dipping of table with compareing root-knot nematode resistant effect
It is as can be seen from Table 2, apparent by performance of the jute seedling that the microbial inoculum is handled in terms of resisting root-knot nematode and infecting
It is better than control, in three concentration gradients are tested, after the microbial inoculum root dipping of 15 times of dilution, root knot number on the root system of seedling single plant
It measures minimum, only 1, this is because 10 times of microbial inoculum of dilution has the hatching of root-knot nematode egg stimulation, and dilutes 20 times
Microbial inoculum the inhibiting effect of root-knot nematode is weakened, therefore, during using microbial inoculum root dipping, preferably extension rate is 15 times.
The optimized designing for fortnulation of 2 micro organism composite fertilizer of experimental example
Optimum Experiment designs five formulas, as shown in table 3.
The constituent content of the micro organism composite fertilizer of 3 different formulations of table
Experiment is altogether there are six handling, the micro organism composite fertilizer and commercially available common compoiste fertilizer that are formulated including above-mentioned five kinds
(control) each handles 3 cells, totally 18 cells, and each processing carries out field management according to a conventional method, specific as follows：Make
Kind of a region is treated in use, being applied in site preparation, per acre using 20 kilograms, when being applied into seedling stage as top dressing, per acre for base manure
Using 20 kilograms, compare six processing and 4 are shown in Table to the control effect of jute root knot nematode disease and the growing state of jute.
4 different disposal of table is to the control effect of jute root knot nematode disease and the growing state of jute
From table 4, it can be seen that compared with the control, the root-knot nematode resistant of three, four, five pairs of jutes of formula has to be prevented well
Effect is controlled, only less than five root knots on root system every plant average, meanwhile, in the cell of these three processing, the growth shape of jute
Condition is good, therefore is formulated three, four and five optimum ratios for the present invention.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (6)
1. a kind of micro organism composite fertilizer for preventing jute root knot nematode disease, which is characterized in that the micro organism composite fertilizer is by as follows
The raw material composition of parts by weight：8~10 parts of the Bacillus cereus microbial inoculum of 20 times of dilution, 990~995 parts of organic fertilizer, inorganic fertilizer 125
~150 parts；
Wherein, the preparation method of the Bacillus cereus microbial inoculum includes the following steps：
Bacillus cercus strain is seeded on PDA plate, through 3 activation, with the single bacterium on transfer needle picking tablet to containing
In the test tube for having PDA culture solutions, kind of a bacterium is made in culture for 24 hours；By 1：Kind of bacterium is connected to three containing PDA culture solutions by 100 ratio
Continue culture in the bottle of angle and microbial inoculum is made for 24 hours；
Said waxy Bacillus is Bacillus cercus AT31, and China Microbiological bacterium is preserved on June 18th, 2009
Kind preservation administration committee common micro-organisms center, culture presevation number are CGMCC NO.3128.
2. application of the micro organism composite fertilizer described in claim 1 in jute root knot nematode disease is prevented.
3. application according to claim 2, which is characterized in that the micro organism composite fertilizer can be used as base manure or top dressing to make
With.
4. application according to claim 3, which is characterized in that the micro organism composite fertilizer is as base manure, per acre using 20
~80kg.
5. application according to claim 3, which is characterized in that the micro organism composite fertilizer shifts to an earlier date 7 as top dressing than chemical fertilizer
Apply within~10 days, per acre using 10~50kg.
6. application according to claim 2, which is characterized in that the micro organism composite fertilizer can be sowed before seed dressing.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1616648A (en) * | 2004-04-14 | 2005-05-18 | 北京天地新生力生物技术有限公司 | Fermentation and preparation for composite bacterial prepn and organic bacterial manure for preventing and controlling soil plant diseases and insect pests |
| CN102154159A (en) * | 2010-12-29 | 2011-08-17 | 南京农业大学 | Antagonistic bacteria for preventing and controlling tomato root-knot nematodes and microorganism organic fertilizer produced by same |
| CN103351198A (en) * | 2013-06-28 | 2013-10-16 | 青岛扎西生物科技有限公司 | High-fertility biofertilizer for crops |
| CN104671961A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Microbial fermentation organic fertilizer for vegetables |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671641B (en) * | 2009-08-12 | 2011-05-11 | 南京农业大学 | Bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease |
| KR101145154B1 (en) * | 2010-04-21 | 2012-05-24 | 이병재 | Agricultral chemicals and producing method thereof |
-
2015
- 2015-09-28 CN CN201510630017.XA patent/CN105218265B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1616648A (en) * | 2004-04-14 | 2005-05-18 | 北京天地新生力生物技术有限公司 | Fermentation and preparation for composite bacterial prepn and organic bacterial manure for preventing and controlling soil plant diseases and insect pests |
| CN102154159A (en) * | 2010-12-29 | 2011-08-17 | 南京农业大学 | Antagonistic bacteria for preventing and controlling tomato root-knot nematodes and microorganism organic fertilizer produced by same |
| CN103351198A (en) * | 2013-06-28 | 2013-10-16 | 青岛扎西生物科技有限公司 | High-fertility biofertilizer for crops |
| CN104671961A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Microbial fermentation organic fertilizer for vegetables |
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Effective date of registration: 20210416 Address after: Room 310-3, headquarters building, Changsha Zhongdian Software Park Co., Ltd., No. 39, Jianshan Road, Changsha hi tech Development Zone, Changsha, Hunan 410000 Patentee after: Hunan Huigu Agricultural Ecology Research Institute Co.,Ltd. Address before: 831500 the Xinjiang Uygur Autonomous Region Changji Hui Autonomous Prefecture, Fukang City Industrial Park Fu Dong Yi Qu Patentee before: XINJIANG TAIGU BIOLOGICAL FERTILIZER Co.,Ltd. |
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