CN105213290A - A kind of Volvariella volvacea (Bull． Ex Franch.) Singer.extract and application thereof - Google Patents
A kind of Volvariella volvacea (Bull． Ex Franch.) Singer.extract and application thereof Download PDFInfo
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Abstract
The invention provides a kind of Volvariella volvacea (Bull． Ex Franch.) Singer.extract and application thereof, described Volvariella volvacea (Bull． Ex Franch.) Singer.extract has good whitening and senile-resistant efficacy, can be used for cosmetics, health food and field of medicaments, especially can be applicable in cosmetics.
Description
Technical field
The invention belongs to field of natural product chemistry, particularly relate to a kind of Volvariella volvacea (Bull． Ex Franch.) Singer.extract and application thereof.
Background technology
Whitening, anti-aging cosmetics are all pursuit and the yearning of women all the time, are also the eternal topic of beauty treatment circle research.In recent years along with socioeconomic development and scientific and technical progress, the demand of people to cosmetics also improves thereupon.
Skin is the organ the most easily appeared in aging course, and skin aging main manifestations is naturally-aged and photoaging two kinds of forms.Skin aging is the coefficient result of endogenous factor and extrinsic factor, and causes of senescence mainly contains that gene are said, free radical is said, neuroendocrine function goes down, and mediate a settlement cross linkage theory, somatic mutation theory, protein synthesis mistake is caused disaster.
The color of human skin is relevant with quantity with the kind of pigment existed in skin, and melanocyte has the greatest impact to skin color.Tryrosinase is melanogenic key enzyme, which control the forming process of melanocyte, and the deposition of its level of activity to pigment plays a major role.Skin chemical whitening agent is certain stage of formation by acting on dermal melanin, metabolic pathway, control, check melanin generate and meet the material of safety requirements, its kind is a lot, mainly active by restraint of tyrosinase or block and produce melanic oxidation reaction from tyrosine, thus reduce melanic generation and reach the effect of skin whitening.
Traditional skin-whitening, antidotal agent, often adopt chemical substance, although these chemical substances have the effect reaching whitening, defying age fast, to skin toxic side effect, life-time service can cause contact dermatitis or cause the ill effects such as permanent decolouring.Therefore, the application of natural product in cosmetics more and more obtains the favor of people.
Volvariella volvacea (Bull.Ex Franch.) Singer． (Volvariellavolvacea (Bull.exFr.) Sing.), another name Nanhua mushroom, delicious Volvariella volvacea (Bull.Ex Franch.) Singer．, delicious luxuriant foot mushroom, Volvariella volvacen, stalk mushroom, numb mushroom and Chinese mushroom etc., because of usually grow humidity rot Caulis et Folium Oryzae in and gain the name, fecund in Guangdong and Guangxi Provinces, Fujian, Jiangxi, Taiwan.Belong to Hymenomycetes (Hymenomycetes), Agaricales (Agricales), Guang Bing mushroom section (Pluteaceae), Volvariella (Volvariella), the specifiable kind of known Volvariella mainly contains the little mushroom of binding foot (V.argentinaSpeg.) of silver color, the little mushroom of binding foot of filamentary silver (V.bombycina (SchaeffFr) .Sing.), edible little mushroom of binding foot (V.esculenta (Mass.) Sing.), the little mushroom of binding foot of Lip river husband (V.loveyana (Gill.exBerk.) Sing.), between little mushroom V.media (Schum.:Fr.) Sing. that binds foot of type), tiny mushroom of binding foot (V.parvula (Weinm.) Speg.)), intend the little mushroom of binding foot of bud (V.pseudovolvacea (Berk.etBr) .Sing.), the hair little mushroom of binding foot of handle (V.pubescentipes (Pk.) Sing.), beautiful little mushroom of binding foot (V.speciosa (Fr:Fr.) Sing.), beautiful little mushroom of binding foot glues lid mutation (V.speciosavar.gloiocephala (DC.:Fr.) Sing.), the hair little mushroom of binding foot of holder (V.villosovolva (LLoyd) Sing.), Volvariella volvacea (Bull.Ex Franch.) Singer． sea nurse mutation (V.volvaceavar.heimiiSing.), Volvariella volvacea (Bull.Ex Franch.) Singer． horse Sa mutation (V.volvaceavar.masseeiSing.), Volvariella volvacea (Bull.Ex Franch.) Singer． var. niger (V.volvaceavar.nigricansKawam.).
Volvariella volvacea (Bull.Ex Franch.) Singer． originates from Guangdong, is a kind of important tropical and subtropical zone mushrooms, and be the third-largest culturing edible fungus in the world, China's Volvariella volvacea (Bull.Ex Franch.) Singer． output occupies first of the world, is mainly distributed in South China.Volvariella volvacea (Bull.Ex Franch.) Singer． is nutritious, delicious flavour, has good health value, and the fresh mushroom of every 100g is containing 207.7mg vitamin C, and 2.6g sugar, 2.68g crude protein, 2.24g is fatty, 0.91g ash.Volvariella volvacea (Bull.Ex Franch.) Singer． protein is containing 18 seed amino acids, and wherein essential amino acids accounts for 40.47-44.47%.In addition, also containing multiple mineral elements such as phosphorus, potassium, calcium.The merit that record according to classic medical book, Volvariella volvacea (Bull.Ex Franch.) Singer． has " heat of dispelling of relieving summer heat, gain is healthy ".Modern medicine is thought, Volvariella volvacea (Bull.Ex Franch.) Singer． has the effects such as invigorating the spleen and replenishing QI, clearing away summer-heat, raising immunity, antitumor, blood sugar lowering, accelerating wound healing.Current Volvariella volvacea (Bull.Ex Franch.) Singer． be mainly used in edible and medicinal on, there is antioxidation, immunity moderation and antitumor action.But the current development arrangement of Volvariella volvacea (Bull.Ex Franch.) Singer． is low, it is narrow to carry out field, and resource is also underutilized, and its medical value and using value thereof lack deeply and comprehensively research.Report there are no about the whitening of Volvariella volvacea (Bull． Ex Franch.) Singer.extract, antidotal effect at present.
Summary of the invention
The object of the invention is to: a kind of Volvariella volvacea (Bull． Ex Franch.) Singer.extract and application thereof are provided, be intended to Volvariella volvacea (Bull． Ex Franch.) Singer.extract to be applied in skin preparations for extenal use, to play the effect of beauty and skin care.
The object of the present invention is achieved like this:
A kind of Volvariella volvacea (Bull． Ex Franch.) Singer.extract, after being pulverized by Volvariella volvacea (Bull.Ex Franch.) Singer．, adds organic solvent extraction, filters, and by filtrate except concentrated after desolventizing, with macroporous resin column absorption, obtains with after water and organic solvent eluting.
Described organic solvent refers to the organic solvent containing hydroxyl or carbonyl isopolarity group, such as: Methanamide, dimethyl formamide, hexamethyl phosphoramide, tetramethylethylenediamine, triethylamine, tri-n-butylamine, acetic acid, trifluoroacetic acid, acetonitrile, methyl formate, ethyl acetate, DMC dimethyl carbonate, dimethyl sulfoxine, acetone, butanone, dioxane, pyridine, oxolane, methanol, ethanol, chloroform, glycerol, propylene glycol, propylene glycol, isopropyl alcohol, n-butyl alcohol and ether etc., in one or more, wherein preferred alcohol aqueous solution.
When taking ethanol water as organic solvent, the concentration of ethanol selects 30-80%.
Known according to test, Volvariella volvacea (Bull． Ex Franch.) Singer.extract has good effect in whitening and defying age, can add in cosmetic formulations as active component, and the active component of indication can provide one or more effect in some cases here.
Active component described in the present invention can also be pantothenylol, glycyrrhizic acid dipotassium, allantoin, nicotiamide, NAP, Herba Violae Hortensis extract, hyaluronate sodium, tocopherol acetas, undecylenoyl phenylalanine etc.In addition, this type of describes is for the ease of understanding, and is not the application or listed application that composition are limited to specifically note.
Volvariella volvacea (Bull． Ex Franch.) Singer.extract can be applicable to skin preparations for extenal use, health food and medicine, especially can be applicable in cosmetics, the consumption different according to the dissimilar interpolation of preparation.
Described Dermatologic preparation composition is the general designation concept of all the components being generally used for skin outside, such as, can be cosmetic combination or pharmaceutical compositions.Can be foundation make up material, facial dressing cosmetic preparation, health cosmetic preparation, hair nursing cosmetic preparation etc. in described cosmetic combination, to its dosage form without particular restriction, can choose reasonable according to different object.
According to the medium difference also allowed containing different cosmeceutical aspects of dosage form and object or matrix excipients in described cosmetic combination.
May be used for the cosmetics of Dermatologic preparation composition of the present invention, dermatological or pharmaceutically acceptable excipient can be the form of aqueous phase, oil phase, gel, water bag wax pattern emulsion, emulsion oil-in-water or water-in-oil emulsion.Aqueous phase is the mixture of one or more water solublity or dispersibility component, and it can be liquid, semisolid or solid under room temperature (25 DEG C).It can be maybe the form of suspension, dispersion liquid or the solution in water or water-ol excipient that excipient comprises, and it can contain thickening agent or gel.The product form that the knowledge-chosen that those skilled in the art can grasp based on those skilled in the art is suitable, the component wherein comprised.
Described compositions can comprise aqueous phase, this aqueous phase can contain the mixture of water or water and at least one hydrophilic organic solvent, described hydrophilic organic solvent such as alcohol, the straight or branched low-grade monobasic alcohol especially containing 2-5 carbon atom, as ethanol or propanol; Polyhydric alcohol, as propylene glycol, sorbitol, glycerol, pantothenylol or Polyethylene Glycol and composition thereof.
When the compositions of inventing is emulsion form, said composition optionally can also comprise surfactant.
Described compositions can also comprise film forming polymer, as polyurethanes, polyacrylic acid homopolymer or copolymer, polyester, based on the resin of hydrocarbon and/or silicone resin.Polymer can be dissolved in or to be scattered in the acceptable excipient of cosmetics and optional and plasticizer merges.
Compositions of the present invention can also comprise oil phase, and described oil phase to contain under room temperature (25 DEG C) for the oil-soluble of liquid or oil-dispersing property component and/or is at room temperature oily or wax-like material, as wax, semisolid, natural gum and composition thereof.This oil phase can also contain organic solvent.
Usually be at room temperature liquid, suitable oily matter comprises: the oil based on hydrocarbon deriving from animal, as perhydro-squalene; Based on the vegetable oil of hydrocarbon, as the triglyceride of the C4-10 fatty acid of liquid, such as enanthic acid or Trivent OCG class, or oil, such as Oleum helianthi, Semen Maydis oil, soybean oil, Oleum Vitis viniferae, Oleum Ricini, American Avocado Tree oil, caprylic/capric triglyceride class, Jojoba oil; The straight or branched hydro carbons in mineral or synthesis source, such as liquid paraffin and its derivant, vaseline; The esters of synthesizing ester and ethers, particularly fatty alcohol, such as isopropyl myristate, Palmic acid 2-Octyl Nitrite, stearic acid 2-octyldodecyl, isostearyl isostearate ester; Hydroxylating esters, the enanthic acid esters of such as lactic acid isooctadecanol ester, octyl hydroxystearate, octyl hydroxystearate, hydroxy stearic acid octyldodecyl, fatty alcohol, sad esters and capric acid lipid; Polyhydric alcohol esters, such as propylene, neopentyl glycol two heptanoate, the different pelargonate of diethylene glycol two and pentaerythritol esters; Aliphatic alcohols containing C12-26, such as octyldodecanol, 2-butyl capryl alcohol, 2-hexyldecanol, 2-undecylpentadecanol, oleyl alcohol; Based on fluorocarbon oil and/or the fluorosilicon oil of part hydrocarbon, silicone oil, be at room temperature liquid or semisolid volatility or nonvolatile straight chain or cyclo-methicone, such as annular dimethyl polysiloxane and polydimethylsiloxane, it optionally comprises phenyl, such as Silicone DC 556, siloxanes and composition thereof.
Compositions of the present invention can comprise any component be usually used in cosmetic field further.These components comprise antiseptic, water phase thickener (extract biopolymer, synthetic polymer) and fatty phase thickening agent, aromatic, hydrophilic and lipophilic active agent and composition thereof.
Compositions of the present invention can also comprise other Particle Phase, and described Particle Phase can be the pigment and/or pearling agent and/or filler that use in cosmetic composition.
Pigment may reside in compositions, and suitable inorganic pigment comprises titanium oxide, zirconium oxide and cerium oxide and zinc oxide, ferrum oxide and ferrum are blue; Suitable organic pigment comprises barium, strontium, calcium and aluminum color lake and carbon black.
Pearling agent may reside in compositions, and suitable pearling agent comprises the Muscovitum being coated with titanium oxide, ferrum oxide or natural pigment.
Filler may reside in compositions, suitable filler comprises Pulvis Talci, silicon dioxide, zinc stearate, Muscovitum, Kaolin, nylon powder, polyethylene powders, Teflon, starch, borazon, copolymer microsphere, such as silicone resin microballon.
The oil phase of the present composition can comprise one or more waxes, natural gum or its mixture.Wax comprises based on the wax of hydrocarbon, fluorine wax and/or siloxane wax, and can derive from plant, mineral, animal and/or synthesis source.Suitable wax comprises Cera Flava, Brazil wax, candelilla wax, paraffin, microwax, ceresine; Synthetic wax comprises Tissuemat E, siloxane wax containing C16-45.Natural gum is generally polydimethylsiloxane or sodium carboxymethyl cellulose or extract class, and semi-solid material is generally the compound based on hydrocarbon, as lanoline and derivant thereof.
Compositions of the present invention can be mixed with any suitable product form.This kind of product form includes, but are not limited to aerosol spray, cream, emulsion, solid, liquid, dispersion, foam, gel, astringent, mousse, ointment, powder, patch, brilliantine, solution, hands by pump-type spray, club, facial film and wet paper towel.Can using compositions of the present invention by various method well-known in the art expediently for the preparation of or as cosmetics, dermatological or medicine local application product.
Dermatologic preparation composition of the present invention can comprise one or more following ingredients: anti-allergic agent, antimicrobial, antioxidant, chelating agen, coloring agent depigmentation, emollient, emulsifying agent, exfoliation agent, film former, spice, wetting agent, insect repellent, lubricant, pharmaceutically active agents, humidizer, photostabilizer, antiseptic, skin protectant, skin penetration enhancer, sunscreen, stabilizing agent, surfactant, thickening agent, viscosity modifier, vitamin or its combination in any.
Accompanying drawing explanation
Fig. 1 is the blank figure suppressing Brachydanio rerio melanin to produce experiment;
Fig. 2 is that Volvariella volvacea (Bull.Ex Franch.) Singer． 30% ethanol position provided by the invention suppresses Brachydanio rerio melanin to produce the design sketch of experiment.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The preparation of embodiment 1 Volvariella volvacea (Bull． Ex Franch.) Singer.extract
The fresh Volvariella volvacea (Bull.Ex Franch.) Singer． of 120g, after pulverizing, add 1200ml70% ethanol (v/v, lower same) supersound extraction, filter, after filtrate is concentrated, loading macroporous resin column, uses water, 30% ethanol, 70% ethanol elution successively, collects eluent respectively, removed under reduced pressure solvent, obtains 30% ethanol extraction position 1.5g, 70% ethanol extraction position 0.5g.
The free radical scavenging Effect study of embodiment 2 Volvariella volvacea (Bull． Ex Franch.) Singer.extract
Get the Volvariella volvacea (Bull.Ex Franch.) Singer． 30% ethanol extraction position of above-mentioned preparation, 70% ethanol extraction position is appropriate, be mixed with aqueous solution with deionized water respectively, pipette 2mL in 10mL tool plug test tube, add people 2mLDPPH alcoholic solution (2*10
-4mol/L), fully mix, room temperature leaves standstill, by the absorbance A of spectrophotometric determination 517nm wavelength after 30 minutes
517(taking ethanol as reference); Simultaneously test 2mLDPPH solution and the mixed absorbance A of 2mL ethanol
c, and 2mL extracting solution and the mixed absorbance A of 2mL alcoholic solution
0.Parallel assay three times, averages, according to following formulae discovery free radical scavenging activity.
Free radical scavenging activity=(1-(A
517-A
0)/A
c) * 100%.
Result of calculation is as shown in table 1.
Table 1
From table 1, Volvariella volvacea (Bull.Ex Franch.) Singer． active site all has the ability of scavenging free radicals, has certain senile-resistant efficacy, wherein 70% ethanol extraction position scavenging free radicals best results.
The tyrosinase inhibitory action research of embodiment 3 Volvariella volvacea (Bull． Ex Franch.) Singer.extract
Get the Volvariella volvacea (Bull.Ex Franch.) Singer． 30% ethanol extraction position of above-mentioned preparation, aqueous solution that 70% ethanol extraction position is configured to 2mg/ml concentration respectively, measure its inhibitory action to tyrosinase activity, to measure its whitening active.Determination step is: set up sample cell, sample controls pipe, positive control, negative control.In sample cell and sample controls pipe, respectively add the sample solution of 1ml variable concentrations, positive and negative control pipe replaces with phosphate buffer.0.5ml tyrosinase solution (Mei Huo unit 125U/ml) is added in sample cell and positive control pipe, sample controls and negative control pipe replace with 0.5ml phosphate buffer (pH=6.8), the all test tubes of jolting, sample and tryrosinase are fully mixed, and 37 DEG C of tanks hatch 10 minutes.Add the 0.03wt% tyrosine solution of 2ml, react 10 minutes, be namely engraved in 475nm place and measure absorbance.
Suppression ratio I (%)=[1-(T-T
0)/(C-C
0)] × 100%
In formula: T
0: sample solvent contrasts;
T: sample controls;
C: positive control;
C
0: positive control solvent control.
Result of the test is as shown in table 2:
Table 2
As can be seen from Table 2,30% ethanol extraction position whitening active of Volvariella volvacea (Bull.Ex Franch.) Singer． is the highest.
Embodiment 4 Volvariella volvacea (Bull． Ex Franch.) Singer.extract suppresses Brachydanio rerio melanin to produce experiment
Get the Volvariella volvacea (Bull.Ex Franch.) Singer． 30% ethanol extraction position of above-mentioned preparation, 10mg/L solution is mixed with deionized water, for Brachydanio rerio whitening test, the raising of Brachydanio rerio and the collection of embryo, Adult Zebrafish is raised in laboratory circulating water culture system, 28.5 DEG C, light/dark cycle is 14/10 hour, and raising water filters and abundant aeration through blood circulation, and pH value keeps about 8.0, every day feeding fairy shrimp three times, feeding 6 days weekly.Selection form is normal, and individual larger and healthy sexual maturity Brachydanio rerio, carries out pairing at hatching system by sex ration 1: 2 and raise in 15L10-15 bar glass aquarium.In 22:00 light-off evening before that day of laying eggs, morning next day, 8:00 system was turned on light automatically, started to lay eggs under light stimulation, collected embryo.The embryo of collection is cleaned fully after removing foul, pick out the zebra fish model that the normal embryo of healthy development sets up screening cosmetics whitening active thing under the microscope: using 96 orifice plates as test chamber, Volvariella volvacea (Bull.Ex Franch.) Singer． 30% ethanol extraction position solution is added in every hole, blank group is set simultaneously, is assigned randomly in 96 orifice plates by 1 embryo/hole.Change 30% ethanol extraction position solution to be measured every day expose liquid and stir, to guarantee that active component is evenly distributed.Pigmentation based on phenotype is assessed, and observe active matter to the melanin inhibition of Brachydanio rerio, the result detected is as shown in table 3, and Brachydanio rerio microscope collection of illustrative plates as shown in Figure 1, 2.
Table 3
As can be seen from Table 3,30% ethanol extraction position of Volvariella volvacea (Bull.Ex Franch.) Singer． has the effect suppressing Brachydanio rerio melanin to be formed, and can be used for skin whitening external preparation.
The skin preparations for extenal use of embodiment 5 containing Volvariella volvacea (Bull． Ex Franch.) Singer.extract
Treating excess syndrome executes Volvariella volvacea (Bull． Ex Franch.) Singer.extract prepared in 1, for the preparation of skin preparations for extenal use.Described skin preparations for extenal use is preferably cosmetic composition, such as astringent, essence, cream etc.The percentage by weight of described Volvariella volvacea (Bull． Ex Franch.) Singer.extract in skin preparations for extenal use is 0.001-1, preferred 0.01-0.2.It is below the embodiment of the embody rule of Volvariella volvacea (Bull． Ex Franch.) Singer.extract in skin preparations for extenal use.In following table, "-" indicates without interpolation.
The astringent of embodiment 6 containing Volvariella volvacea (Bull． Ex Franch.) Singer.extract
Get Volvariella volvacea (Bull． Ex Franch.) Singer.extract, for the preparation of the astringent with defying age, whitening function, the component that described astringent contains is as shown in table 4:
Table 4
The essence of embodiment 7 containing Volvariella volvacea (Bull． Ex Franch.) Singer.extract
Get Volvariella volvacea (Bull． Ex Franch.) Singer.extract, for the preparation of the essence with defying age, whitening function, the component that described essence contains is as shown in table 5:
Table 5
Emulsion/the frost of embodiment 8 containing Volvariella volvacea (Bull． Ex Franch.) Singer.extract
Get Volvariella volvacea (Bull． Ex Franch.) Singer.extract, for the preparation of the emulsion/frost with defying age, whitening function, the component that described emulsion/frost contains is as shown in table 6:
Table 6
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (11)
1. a Volvariella volvacea (Bull． Ex Franch.) Singer.extract, is characterized in that, after being pulverized by Volvariella volvacea (Bull.Ex Franch.) Singer．, adds organic solvent extraction, filters, and by filtrate except concentrated after desolventizing, after macroporous resin adsorption, with water and organic solvent eluting, obtains Volvariella volvacea (Bull． Ex Franch.) Singer.extract.
2. Volvariella volvacea (Bull． Ex Franch.) Singer.extract as claimed in claim 1, it is characterized in that, described organic solvent is ethanol.
3. Volvariella volvacea (Bull． Ex Franch.) Singer.extract as claimed in claim 2, is characterized in that, after being pulverized by Volvariella volvacea (Bull.Ex Franch.) Singer．, add 70% ethanol ultrasonic extraction, filter, by filtrate except concentrated after desolventizing, by macroporous resin adsorption, use water, 30% ethanol, 70% ethanol elution successively, obtain after eluent removed under reduced pressure solvent.
4. the Volvariella volvacea (Bull． Ex Franch.) Singer.extract as described in as arbitrary in claim 1-3 has whitening function.
5. the Volvariella volvacea (Bull． Ex Franch.) Singer.extract as described in as arbitrary in claim 1-3 has restraint of tyrosinase activity.
6. the Volvariella volvacea (Bull． Ex Franch.) Singer.extract as described in as arbitrary in claim 1-3 has anti-aging effects.
7. one kind contains the Dermatologic preparation composition of Volvariella volvacea (Bull． Ex Franch.) Singer.extract as effective ingredient.
8. Dermatologic preparation composition as claimed in claim 7, is characterized in that, described Volvariella volvacea (Bull． Ex Franch.) Singer.extract passes through after Volvariella volvacea (Bull.Ex Franch.) Singer． pulverizing, add organic solvent extraction, filter, by filtrate except concentrated after desolventizing, after macroporous resin adsorption, obtain with water and organic solvent eluting.
9. Dermatologic preparation composition as claimed in claim 8, it is characterized in that, described Volvariella volvacea (Bull． Ex Franch.) Singer.extract passes through after Volvariella volvacea (Bull.Ex Franch.) Singer． pulverizing, add 70% ethanol ultrasonic extraction, filter, by filtrate except concentrated after desolventizing, by macroporous resin adsorption, use water, 30% ethanol, 70% ethanol elution successively, eluent removed under reduced pressure solvent is obtained.
10. Dermatologic preparation composition as claimed in claim 8 or 9, it is characterized in that, the percentage by weight of described Volvariella volvacea (Bull． Ex Franch.) Singer.extract in Dermatologic preparation composition is 0.001-1.
11. Dermatologic preparation compositions as claimed in claim 10, it is characterized in that, the percentage by weight of described Volvariella volvacea (Bull． Ex Franch.) Singer.extract in Dermatologic preparation composition is 0.01-0.2.
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| CN110072397B (en) * | 2016-12-13 | 2023-03-10 | 联合利华知识产权控股有限公司 | Aerated food product |
| US11785964B2 (en) | 2016-12-13 | 2023-10-17 | Conopco, Inc. | Aerated food products |
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