CN105211598A - A kind of preparation method of Soybean Peptide albumen pig feed - Google Patents
A kind of preparation method of Soybean Peptide albumen pig feed Download PDFInfo
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- CN105211598A CN105211598A CN201510791733.6A CN201510791733A CN105211598A CN 105211598 A CN105211598 A CN 105211598A CN 201510791733 A CN201510791733 A CN 201510791733A CN 105211598 A CN105211598 A CN 105211598A
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Abstract
The invention discloses a kind of preparation method of Soybean Peptide albumen pig feed, belong to feed preparation field.The present invention be get soybean sieve cleaning mill after boiling, be separated to obtain bean dregs, collect slurries for subsequent use, repeatedly roast air-dry for bean dregs surface, cool for subsequent use, get fresh pig small intestine, cleaning chitterlings, cut off with scissors, scraping mucus, sterilizing obtains chitterlings gastric juice, mix with the bean dregs after roasting, then add mould, add slurries after preservation to be sealed is mouldy and soak, stirring staticly settles, and after filtration, sunshine is exposed to the sun, pulverizer is pulverized, oven drying obtains.The invention has the beneficial effects as follows: preparation method of the present invention is simple, production process controllability is strong, and the Soybean Peptide molecular distribution of production is even, and molecular weight is little; Products obtained therefrom ANFs content is low, and nutrient is high, and result of use is good, good stability.
Description
Technical field
The present invention relates to a kind of preparation method of Soybean Peptide albumen pig feed, belong to feed preparation field.
Background technology
Soybean Peptide protein feed, is commonly called as fermented bean dregs, has another name called biological peptide, biological soybean meal, bioactive micro peptide, Soybean Peptide, soya-bean polypeptides.Because title is more and chaotic, for being different from the contained function of soybean oligopeptide and the attribute as high-quality protein raw material thereof in treatment, Soybean Peptide for health care and outstanding fermented bean dregs, the title of Soybean Peptide protein feed is more appropriate.So-called fermented bean dregs, utilize modern biological project fermented bacterium technology exactly, with high-quality dregs of beans for primary raw material, pass through fermentable, eliminate ANFs wherein to greatest extent, and soybean protein is degraded to the mixture of soluble protein and micromolecule polypeptide, produce the materials such as a large amount of probio, oligopeptides, glutamic acid, lactic acid, vitamin, UGF simultaneously, be particularly suitable for young animal (as lactation pigling, wean pigling, child care pig) use in feed, effect is fine.Soybean Peptide good water solubility, has good water-disintegrable, good heat endurance and acid resistance, absorption easy to digest, by blood pressure, can promote fat and mineral absorption, regulate blood sugar concentration, be widely used in cultivating industry.
The method of current production Soybean Peptide protein feed has chemical hydrolysis, enzymatic isolation method and microbe fermentation method, chemical hydrolysis it be the peptide bond fission utilizing chemical reagent to make in soybean protein molecule at a certain temperature, thus form small-molecule substance.The method production cost is low, process simple, but whole technical process is difficult to control, and the Soybean Peptide molecular distribution of production is uneven, physiological function difference is large, and a large amount of amino acid such as threonine, serine can be destroyed, there is racemization, nutrition is run off in a large number, is not suitable for large-scale production.Enzymatic isolation method mainly utilizes protease at specific temperature and pH condition, can be hydrolyzed efficiently soybean protein molecule, soybean protein molecular degradation is become small-molecule peptide, but because degree of hydrolysis is not high, in hydrolytic process, easily produce bitter taste, enzyme expensive, cause Soybean Peptide yield low, preparation process is complicated, and production cost is high.Microbe fermentation method utilizes the enzyme that in sweat, Institute of Micro-biology produces that soybean protein molecular degradation is become small protein molecule, and the method cost is low, but poor stability, the production time is long, and production process is wayward.Therefore work out that a kind of production process is easy to control, the preparation method of the measured Soybean Peptide protein feed of matter on this basis, the development of feedstuff industry is had great importance.
Summary of the invention
Technical problem to be solved by this invention: higher for current Soybean Peptide protein feed ANFs content, molecular weight is bigger than normal, animal physiological performance and growth performance is caused to reduce, prepared by employing chemical hydrolysis, production process poor controllability, the Soybean Peptide molecular distribution of producing is uneven, physiological function difference is large, and can threonine be destroyed, a large amount of amino acid such as serine, there is racemization, nutrition is run off in a large number, prepared by enzymatic isolation method, because degree of hydrolysis is not high, easily bitter taste is produced in hydrolytic process, enzyme expensive, cause Soybean Peptide yield low, preparation process is complicated, production cost is high, product stability is poor, the drawback that production time is long, provide a kind of preparation method of Soybean Peptide albumen pig feed, the present invention gets the boiling of washing soybean defibrination, be separated to obtain bean dregs, collection slurries are for subsequent use, bean dregs are roasted repeatedly, add chitterlings gastric juice after cooling to mix, then mould is added, add slurries after mouldy to soak, reach the object improving Soybean Peptide yield.Preparation process of the present invention is simple, and products obtained therefrom good stability, ANFs content is lower.
For solving the problems of the technologies described above, the present invention adopts technical scheme as described below to be:
(1) getting 2 ~ 3kg soybean, is 10 ~ 15 μm of screen clothes with aperture, sieves 2 ~ 3 times, puts into water and repeatedly rubs cleaning 2 ~ 3 times, removes spot and impurity, then soaks 5 ~ 7h in clear water, after immersion completes, slightly rinses 1 time with current;
(2) soybean rinsed is put into stone mill, manually carry out milling 1 ~ 2h, obtaining particle diameter is 2 ~ 5mm soybean slag, collects the juice of milling out, the juice of soybean slag and collection is placed in circular bottom pot simultaneously, add water wherein, make it not have soybean slag 5 ~ 7cm, cover pot cover, be then warming up to 100 ~ 110 DEG C with the speed program of 10 DEG C/min, boiling 20 ~ 30min
(3) after above-mentioned boiling completes, water is added in pot, addition and soybean slag mass ratio are 3:1, after repeatedly turning over and copy with slice bottom from pot and mix again, slow fire boiling 30 ~ 40min, be cooled to 20 ~ 25 DEG C with the speed program of 5 DEG C/min again, take out the bean dregs carrying out isolated by filtration and must cook, collect slurries for subsequent use;
(4) bean dregs after the cooking of above-mentioned gained are placed in ventilation, turn over dry in the air once every 30min, after its surface is air-dry, be placed in stove, control temperature is 90 ~ 95 DEG C, repeatedly roasts 40 ~ 50min, to be roasted complete after, take out and be cooled to room temperature, for subsequent use;
(5) get 1 ~ 2kg fresh pig small intestine clear water and soak 20 ~ 30min, 1 ~ 2 time is slightly rinsed again with current, after taking out natural air drying, cut off along small intestine side with scissors, paved, by the mucus of the stainless steel substrates that 2 ~ 3cm is thick slight scraping small intestine inside, with being placed on disinfection 30 ~ 40min under uviol lamp, obtain chitterlings gastric juice;
(6) the chitterlings gastric juice after above-mentioned disinfection is put into for subsequent use bean dregs roasted, control rotating speed is 500 ~ 700r/min, stir 30 ~ 40min, mix and put into closed container, in closed container, add 2 ~ 3g aspergillus oryzae again, control temperature is 35 ~ 40 DEG C, sealing preservation 3 ~ 5 days, improving temperature is 45 ~ 65 DEG C, preserves 5 ~ 7 days;
(7) after above-mentioned preservation mixture is mouldy, add the slurries for subsequent use of above-mentioned collection, it is made not have mixture 2 ~ 3cm, soak 5 ~ 7 days, after it has soaked, control rotating speed is 800 ~ 1000r/min, magnetic agitation 30 ~ 40min, leave standstill 1 ~ 2h, isolated by filtration obtains filtrate, be exposed to the sun under being placed in sunlight 1 ~ 2 day, ted once every 3 ~ 4h, then put into pulverizer and pulverize, then be placed in the dry 50 ~ 60min of drying box, room temperature is cooled to, i.e. obtained a kind of Soybean Peptide albumen pig feed under ventilation state.
Application process of the present invention: add in normal diet using obtained Soybean Peptide albumen pig feed as additive, addition is 3 ~ 5g/kg, feeding piglet, the every day of each interpolation sooner or later once, after 8 ~ 13 days, piglet daily gain improves 10 ~ 15%, and feed intake improves 10 ~ 14%.Effective promotion piglet health status, improves piglet immunological function.
The present invention is compared with additive method, and Advantageous Effects is:
(1) preparation method of the present invention is simple, and production process controllability is strong, and the Soybean Peptide molecular distribution of production is even, and molecular weight is little;
(2) products obtained therefrom ANFs content is low, and nutrient is high, and result of use is good, good stability, absorption easy to digest.
Detailed description of the invention
First getting 2 ~ 3kg soybean, is 10 ~ 15 μm of screen clothes with aperture, sieves 2 ~ 3 times, puts into water and repeatedly rubs cleaning 2 ~ 3 times, removes spot and impurity, then soaks 5 ~ 7h in clear water, after immersion completes, slightly rinses 1 time with current, then the soybean rinsed is put into stone mill, manually carry out milling 1 ~ 2h, obtaining particle diameter is 2 ~ 5mm soybean slag, collect the juice of milling out, the juice of soybean slag and collection is placed in circular bottom pot simultaneously, add water wherein, it is made not have soybean slag 5 ~ 7cm, cover pot cover, then 100 ~ 110 DEG C are warming up to the speed program of 10 DEG C/min, boiling 20 ~ 30min, after above-mentioned boiling completes, water is added in pot, addition and soybean slag mass ratio are 3:1, after repeatedly turning over and copy with slice bottom from pot and mix again, slow fire boiling 30 ~ 40min, 20 ~ 25 DEG C are cooled to again with the speed program of 5 DEG C/min, take out the bean dregs carrying out isolated by filtration and must cook, collection slurries are for subsequent use, again the bean dregs after the cooking of above-mentioned gained are placed in ventilation, turn over dry in the air once every 30min, after its surface is air-dry, be placed in stove, control temperature is 90 ~ 95 DEG C, repeatedly roasts 40 ~ 50min, to be roasted complete after, take out and be cooled to room temperature, for subsequent use, get 1 ~ 2kg fresh pig small intestine clear water again and soak 20 ~ 30min, 1 ~ 2 time is slightly rinsed again with current, after taking out natural air drying, cut off along small intestine side with scissors, paved, by the mucus of the stainless steel substrates that 2 ~ 3cm is thick slight scraping small intestine inside, with being placed on disinfection 30 ~ 40min under uviol lamp, obtain chitterlings gastric juice, then the chitterlings gastric juice after above-mentioned disinfection is put into for subsequent use bean dregs roasted, control rotating speed is 500 ~ 700r/min, stir 30 ~ 40min, mix and put into closed container, in closed container, add 2 ~ 3g aspergillus oryzae again, control temperature is 35 ~ 40 DEG C, sealing preservation 3 ~ 5 days, improving temperature is 45 ~ 65 DEG C, preserves 5 ~ 7 days, finally after above-mentioned preservation mixture is mouldy, add the slurries for subsequent use of above-mentioned collection, it is made not have mixture 2 ~ 3cm, soak 5 ~ 7 days, after it has soaked, control rotating speed is 800 ~ 1000r/min, magnetic agitation 30 ~ 40min, leave standstill 1 ~ 2h, isolated by filtration obtains filtrate, be exposed to the sun under being placed in sunlight 1 ~ 2 day, ted once every 3 ~ 4h, then put into pulverizer and pulverize, then be placed in the dry 50 ~ 60min of drying box, room temperature is cooled to, i.e. obtained a kind of Soybean Peptide albumen pig feed under ventilation state.
Example 1
First getting 2kg soybean, is 10 μm of screen clothes with aperture, sieves 2 times, puts into water and repeatedly rubs cleaning 2 times, removes spot and impurity, then soak 5h in clear water, after immersion completes, slightly rinse 1 time with current, then the soybean rinsed is put into stone mill, manually carry out milling 1h, obtaining particle diameter is 2mm soybean slag, collect the juice of milling out, the juice of soybean slag and collection is placed in circular bottom pot simultaneously, add water wherein, it is made not have soybean slag 5cm, cover pot cover, then 100 DEG C are warming up to the speed program of 10 DEG C/min, boiling 20min, after above-mentioned boiling completes, water is added in pot, addition and soybean slag mass ratio are 3:1, after repeatedly turning over and copy with slice bottom from pot and mix again, slow fire boiling 30min, 20 DEG C are cooled to again with the speed program of 5 DEG C/min, take out the bean dregs carrying out isolated by filtration and must cook, collection slurries are for subsequent use, again the bean dregs after the cooking of above-mentioned gained are placed in ventilation, turn over dry in the air once every 30min, after its surface is air-dry, be placed in stove, control temperature is 90 DEG C, repeatedly roasts 40min, to be roasted complete after, take out and be cooled to room temperature, for subsequent use, get 1kg fresh pig small intestine clear water again and soak 20min, 1 time is slightly rinsed again with current, after taking out natural air drying, cut off along small intestine side with scissors, paved, by the mucus of the stainless steel substrates that 2cm is thick slight scraping small intestine inside, with being placed on disinfection 30min under uviol lamp, obtain chitterlings gastric juice, then the chitterlings gastric juice after above-mentioned disinfection is put into for subsequent use bean dregs roasted, control rotating speed is 500r/min, stir 30min, mix and put into closed container, in closed container, add 2g aspergillus oryzae again, control temperature is 35 DEG C, sealing preservation 3 days, improving temperature is 45 DEG C, preserves 5 days, finally after above-mentioned preservation mixture is mouldy, add the slurries for subsequent use of above-mentioned collection, make it not have mixture 2cm, soak 5 days, after it has soaked, control rotating speed has been 800r/min, magnetic agitation 30min, leave standstill 1h, isolated by filtration obtains filtrate, be exposed to the sun under being placed in sunlight 1 day, ted once every 3h, then put into pulverizer and pulverize, then be placed in the dry 50min of drying box, room temperature is cooled to, i.e. obtained a kind of Soybean Peptide albumen pig feed under ventilation state.
Add in normal diet using obtained Soybean Peptide albumen pig feed as additive, addition is 3g/kg, feeding piglet, and once, after 8 days, piglet daily gain improves 10% to the every day of each interpolation sooner or later, and feed intake improves 10%.Effective promotion piglet health status, improves piglet immunological function.
Example 2
First getting 2.5kg soybean, is 12 μm of screen clothes with aperture, sieves 2 times, puts into water and repeatedly rubs cleaning 2 times, removes spot and impurity, then soak 6h in clear water, after immersion completes, slightly rinse 1 time with current, then the soybean rinsed is put into stone mill, manually carry out milling 1.5h, obtaining particle diameter is 3.5mm soybean slag, collect the juice of milling out, the juice of soybean slag and collection is placed in circular bottom pot simultaneously, add water wherein, it is made not have soybean slag 6cm, cover pot cover, then 105 DEG C are warming up to the speed program of 10 DEG C/min, boiling 25min, after above-mentioned boiling completes, water is added in pot, addition and soybean slag mass ratio are 3:1, after repeatedly turning over and copy with slice bottom from pot and mix again, slow fire boiling 35min, 22 DEG C are cooled to again with the speed program of 5 DEG C/min, take out the bean dregs carrying out isolated by filtration and must cook, collection slurries are for subsequent use, again the bean dregs after the cooking of above-mentioned gained are placed in ventilation, turn over dry in the air once every 30min, after its surface is air-dry, be placed in stove, control temperature is 92 DEG C, repeatedly roasts 45min, to be roasted complete after, take out and be cooled to room temperature, for subsequent use, get 1.5kg fresh pig small intestine clear water again and soak 25min, 2 times are slightly rinsed again with current, after taking out natural air drying, cut off along small intestine side with scissors, paved, by the mucus of the stainless steel substrates that 2.5cm is thick slight scraping small intestine inside, with being placed on disinfection 35min under uviol lamp, obtain chitterlings gastric juice, then the chitterlings gastric juice after above-mentioned disinfection is put into for subsequent use bean dregs roasted, control rotating speed is 600r/min, stir 35min, mix and put into closed container, in closed container, add 2.5g aspergillus oryzae again, control temperature is 38 DEG C, sealing preservation 4 days, improving temperature is 55 DEG C, preserves 6 days, finally after above-mentioned preservation mixture is mouldy, add the slurries for subsequent use of above-mentioned collection, make it not have mixture 2.5cm, soak 6 days, after it has soaked, control rotating speed has been 900r/min, magnetic agitation 35min, leave standstill 1.5h, isolated by filtration obtains filtrate, be exposed to the sun under being placed in sunlight 2 days, ted once every 3.5h, then put into pulverizer and pulverize, then be placed in the dry 55min of drying box, room temperature is cooled to, i.e. obtained a kind of Soybean Peptide albumen pig feed under ventilation state.
Add in normal diet using obtained Soybean Peptide albumen pig feed as additive, addition is 4g/kg, feeding piglet, and once, after 10 days, piglet daily gain improves 13% to the every day of each interpolation sooner or later, and feed intake improves 12%.Effective promotion piglet health status, improves piglet immunological function.
Example 3
First getting 3kg soybean, is 15 μm of screen clothes with aperture, sieves 3 times, puts into water and repeatedly rubs cleaning 3 times, removes spot and impurity, then soak 7h in clear water, after immersion completes, slightly rinse 1 time with current, then the soybean rinsed is put into stone mill, manually carry out milling 2h, obtaining particle diameter is 5mm soybean slag, collect the juice of milling out, the juice of soybean slag and collection is placed in circular bottom pot simultaneously, add water wherein, it is made not have soybean slag 7cm, cover pot cover, then 110 DEG C are warming up to the speed program of 10 DEG C/min, boiling 30min, after above-mentioned boiling completes, water is added in pot, addition and soybean slag mass ratio are 3:1, after repeatedly turning over and copy with slice bottom from pot and mix again, slow fire boiling 40min, 25 DEG C are cooled to again with the speed program of 5 DEG C/min, take out the bean dregs carrying out isolated by filtration and must cook, collection slurries are for subsequent use, again the bean dregs after the cooking of above-mentioned gained are placed in ventilation, turn over dry in the air once every 30min, after its surface is air-dry, be placed in stove, control temperature is 95 DEG C, repeatedly roasts 50min, to be roasted complete after, take out and be cooled to room temperature, for subsequent use, get 2kg fresh pig small intestine clear water again and soak 30min, 2 times are slightly rinsed again with current, after taking out natural air drying, cut off along small intestine side with scissors, paved, by the mucus of the stainless steel substrates that 3cm is thick slight scraping small intestine inside, with being placed on disinfection 40min under uviol lamp, obtain chitterlings gastric juice, then the chitterlings gastric juice after above-mentioned disinfection is put into for subsequent use bean dregs roasted, control rotating speed is 700r/min, stir 40min, mix and put into closed container, in closed container, add 3g aspergillus oryzae again, control temperature is 40 DEG C, sealing preservation 5 days, improving temperature is 65 DEG C, preserves 7 days, finally after above-mentioned preservation mixture is mouldy, add the slurries for subsequent use of above-mentioned collection, make it not have mixture 3cm, soak 7 days, after it has soaked, control rotating speed has been 1000r/min, magnetic agitation 40min, leave standstill 2h, isolated by filtration obtains filtrate, be exposed to the sun under being placed in sunlight 2 days, ted once every 4h, then put into pulverizer and pulverize, then be placed in the dry 60min of drying box, room temperature is cooled to, i.e. obtained a kind of Soybean Peptide albumen pig feed under ventilation state.
Add in normal diet using obtained Soybean Peptide albumen pig feed as additive, addition is 5g/kg, feeding piglet, and once, after 13 days, piglet daily gain improves 15% to the every day of each interpolation sooner or later, and feed intake improves 14%.Effective promotion piglet health status, improves piglet immunological function.
Claims (1)
1. a preparation method for Soybean Peptide albumen pig feed, is characterized in that concrete preparation process is:
(1) getting 2 ~ 3kg soybean, is 10 ~ 15 μm of screen clothes with aperture, sieves 2 ~ 3 times, puts into water and repeatedly rubs cleaning 2 ~ 3 times, removes spot and impurity, then soaks 5 ~ 7h in clear water, after immersion completes, slightly rinses 1 time with current;
(2) soybean rinsed is put into stone mill, manually carry out milling 1 ~ 2h, obtaining particle diameter is 2 ~ 5mm soybean slag, collects the juice of milling out, the juice of soybean slag and collection is placed in circular bottom pot simultaneously, add water wherein, make it not have soybean slag 5 ~ 7cm, cover pot cover, be then warming up to 100 ~ 110 DEG C with the speed program of 10 DEG C/min, boiling 20 ~ 30min
(3) after above-mentioned boiling completes, water is added in pot, addition and soybean slag mass ratio are 3:1, after repeatedly turning over and copy with slice bottom from pot and mix again, slow fire boiling 30 ~ 40min, be cooled to 20 ~ 25 DEG C with the speed program of 5 DEG C/min again, take out the bean dregs carrying out isolated by filtration and must cook, collect slurries for subsequent use;
(4) bean dregs after the cooking of above-mentioned gained are placed in ventilation, turn over dry in the air once every 30min, after its surface is air-dry, be placed in stove, control temperature is 90 ~ 95 DEG C, repeatedly roasts 40 ~ 50min, to be roasted complete after, take out and be cooled to room temperature, for subsequent use;
(5) get 1 ~ 2kg fresh pig small intestine clear water and soak 20 ~ 30min, 1 ~ 2 time is slightly rinsed again with current, after taking out natural air drying, cut off along small intestine side with scissors, paved, by the mucus of the stainless steel substrates that 2 ~ 3cm is thick slight scraping small intestine inside, with being placed on disinfection 30 ~ 40min under uviol lamp, obtain chitterlings gastric juice;
(6) the chitterlings gastric juice after above-mentioned disinfection is put into for subsequent use bean dregs roasted, control rotating speed is 500 ~ 700r/min, stir 30 ~ 40min, mix and put into closed container, in closed container, add 2 ~ 3g aspergillus oryzae again, control temperature is 35 ~ 40 DEG C, sealing preservation 3 ~ 5 days, improving temperature is 45 ~ 65 DEG C, preserves 5 ~ 7 days;
(7) after above-mentioned preservation mixture is mouldy, add the slurries for subsequent use of above-mentioned collection, it is made not have mixture 2 ~ 3cm, soak 5 ~ 7 days, after it has soaked, control rotating speed is 800 ~ 1000r/min, magnetic agitation 30 ~ 40min, leave standstill 1 ~ 2h, isolated by filtration obtains filtrate, be exposed to the sun under being placed in sunlight 1 ~ 2 day, ted once every 3 ~ 4h, then put into pulverizer and pulverize, then be placed in the dry 50 ~ 60min of drying box, room temperature is cooled to, i.e. obtained a kind of Soybean Peptide albumen pig feed under ventilation state.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490371A (en) * | 2016-12-16 | 2017-03-15 | 常州市鼎日环保科技有限公司 | A kind of preparation method of many nutrient laden palatability Semen sojae atricolor peptide protein pig feeds |
| CN106490367A (en) * | 2016-11-22 | 2017-03-15 | 常州思宇知识产权运营有限公司 | A kind of preparation method of high nutrition Semen sojae atricolor peptide protein pig feed |
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2015
- 2015-11-17 CN CN201510791733.6A patent/CN105211598A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490367A (en) * | 2016-11-22 | 2017-03-15 | 常州思宇知识产权运营有限公司 | A kind of preparation method of high nutrition Semen sojae atricolor peptide protein pig feed |
| CN106490371A (en) * | 2016-12-16 | 2017-03-15 | 常州市鼎日环保科技有限公司 | A kind of preparation method of many nutrient laden palatability Semen sojae atricolor peptide protein pig feeds |
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