CN105199941A - Device for removing cumulus cells - Google Patents
Device for removing cumulus cells Download PDFInfo
- Publication number
- CN105199941A CN105199941A CN201510658335.7A CN201510658335A CN105199941A CN 105199941 A CN105199941 A CN 105199941A CN 201510658335 A CN201510658335 A CN 201510658335A CN 105199941 A CN105199941 A CN 105199941A
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- small opening
- ware
- cumulus
- cumulus cell
- pin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0081—Purging biological preparations of unwanted cells
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- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a device for removing cumulus cells. The device comprises a leak-hole dish, a blowing needle and an airbag; the leak-hole dish is in a circular shape, leak holes for removing the cumulus cells are uniformly formed in the bottom of the dish, the diameter of the leak hole is 90 to 150 micrometers, and inner threads are arranged in the lea holes; the blowing needle is provided with a handle, a tip of the blowing needle is round and smooth, and the blowing needle is connected with the airbag by virtue of an air pipe. The device is simple to operate, high in efficiency and capable of saving the labor; the cumulus cells are mechanically removed, so that the harm on the cumulus cells is alleviated; the consumption time is short, and the survival rate of the cumulus cell is increased.
Description
Technical field
The present invention relates to one and remove cumulus cell device.
Background technology
Ovocyte is commonly called as ovum, and ovocyte is wrapped up by cumulus cell in growth course in early days, and form cumulus oocyte mixture, after Oocyte Development maturation, the cumulus cell around ovocyte completes its physiological function.In order to further investigate the genesis and development mechanism of ovocyte in many researchs such as reproductive development, need the cumulus cell removed around ovocyte.At present, the cumulus cell removed around ovocyte mainly passes through to add Unidasa in operation liquid, Unidasa is as commercialization biological reagent, its biologically active labile, requires higher to storage requirement, easily damages ovocyte during digestion cumulus cell, and, also need the artificial piping and druming of machinery after adding Unidasa, if piping and druming dynamics may cause can not taking off cumulus cell easily not, dynamics is excessive can cause again ovocyte injury or dead; Take artificial mechanism to blow and beat and remove cumulus cell, at present owing to lacking the operating gear of specialty, operate more consuming time and efficiency is lower, when operating time is long, ovocyte is longer in the extraneous operating environment time, easily causes ovocyte mortality or affects the growth of ovocyte later stage.For this problem, the present invention designs the new cumulus cell device that removes and solves this difficult problem.
Summary of the invention
The technical problem to be solved in the present invention is to provide one and removes cumulus cell device.
The another one technical problem that the present invention will solve is to provide one and removes cumulus cell method.
For removing cumulus cell device, the technical solution used in the present invention is, one removes cumulus cell device, comprises small opening ware, piping and druming pin, air bag;
Small opening ware is circular, the uniform small opening removing cumulus cell at the bottom of ware, and small opening diameter is 90 μm ~ 150 μm, is provided with internal thread in small opening;
Piping and druming pin is provided with handle, and the needle point of piping and druming pin is round and smooth, and piping and druming pin is connected with air bag by tracheae.
As preferably, small opening diameter is respectively 150 μm, 120 μm, 90 μm, and the small opening of 3 kinds of different diameters is uniformly distributed by diameter from the center of circle at the bottom of circular ware from big to small from inside to outside along radius.
As preferably, at the bottom of the ware of small opening ware, be provided with the circular pit for connecting culture dish.
As preferably, the diameter of small opening ware is 66mm, and the circular pit diameter at the bottom of ware is 60mm, for connecting 60mm culture dish.
As preferably, the tip diameter of piping and druming pin is set as 120 μm ~ 160 μm.
As preferably, air bag is that pin jams on contracting air-generating.
As preferably, tracheae comprises skin and internal layer, and skin is protective layer, and internal layer is induction pipe, and the length of tracheae is 1.5 ~ 2m.
As preferably, also comprise Cleaning tool made of plastic; Cleaning tool top is handle, and bottom is piston, and handle is connected with in the middle of piston; The internal diameter of piston diameter and small opening ware is suitable, and piston ridge is provided with multi-layer groove.
As preferably, small opening ware is that glass is made.
For removing ovocyte method, the technical solution used in the present invention is, comprises the following steps:
(1) in small opening ware, add nutrient solution, moved in small opening ware by washed cumulus oocyte mixture, rock small opening ware all around, object makes cumulus oocyte mixture be uniformly distributed;
(2) annular recesses at the bottom of the small opening ware ware of immigration cumulus oocyte mixture is placed in above a culture dish, adds in culture dish and operate liquid in right amount;
(3) under cumulus oocyte mixture being put into microscope, the handle of hand-held piping and druming pin, pin steps on air bag, first one section of liquid is sucked, then find object cumulus oocyte mixture under the microscope, pin and object cumulus oocyte mixture will be blown and beaten, small opening three alignment at the bottom of small opening ware ware, jam on air bag, object cumulus oocyte mixture is blown into the small opening removing cumulus cell by piping and druming pin, the scraping retardation of concavo-convex staggered screw thread on small opening inwall, the cumulus cell be attached to around ovocyte is come off and is separated around ovocyte, ovocyte after separation and part cumulus cell finally fall into and the culture dish be connected bottom small opening ware, carry out subsequent operations,
(4) choose the immigration of the ovocyte after having removed cumulus cell nutrient solution to cultivate;
(5) drop in small opening ware is outwelled, add distilled water and clean for subsequent use.
The invention has the beneficial effects as follows:
1, simple to operate, efficiency is high, saves manpower;
2, mechanical removal cumulus cell, reduces the injury to ovocyte;
3, consuming time short, improve ovocyte surviving rate.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
Fig. 1 is the small opening ware front view that the present invention removes cumulus cell device embodiment.
Fig. 2 is the small opening ware vertical view that the present invention removes cumulus cell device embodiment.
Fig. 3 is the piping and druming needle construction schematic diagram that the present invention removes cumulus cell device embodiment.
Fig. 4 is the Cleaning tool structural representation that the present invention removes cumulus cell device embodiment.
Fig. 5 is the use schematic diagram that the present invention removes cumulus cell device embodiment.
Mark in figure, 1-small opening ware, 2-macropore, 3-mesopore, 4-aperture, 5-syringe needle, 6-handle, 7-tracheae, 8-air bag, 9-piston, 10-groove, 11-handle, 12-culture dish.
Embodiment
Fig. 5 is that one removes cumulus cell device, is made up of small opening ware, piping and druming pin, air bag.
Small opening ware 1 is as shown in Figure 1 the circular glass ware of diameter 66mm, is provided with circular pit at the bottom of the ware of small opening ware, the diameter of circular pit be 60mm and be the same center of circle at the bottom of ware, circular pit is for connecting the culture dish of 60mm diameter.The uniform small opening removing cumulus cell at the bottom of small opening ware ware, small opening be respectively the macropore 2 of diameter 150 μm, the mesopore 3 of diameter 120 μm, diameter 90 μm the small opening of aperture 4,3 kinds of different diameters be uniformly distributed from inside to outside from big to small (Fig. 2) by diameter from the center of circle at the bottom of circular ware along radius.
In small opening, diameter is that the macropore of 150 μm is suitable for ovocyte diameter (comprising zona pellucida around ovocyte) between 120 μm ~ 150 μm, diameter is that the mesopore of 120 μm is suitable for the diameter (comprising zona pellucida around ovocyte) of ovocyte between 90 μm ~ 120 μm, and diameter is that 90 μm of diameters (comprising zona pellucida around ovocyte) being suitable for ovocyte are between 60 μm ~ 90 μm.At present, common laboratory animal ovocyte diameter (comprising zona pellucida around ovocyte) is between 60 μm ~ 150 μm.
In small opening, be provided with internal thread, the screw thread in small opening has the function of mechanical removal ovocyte surrounding cumulus cells, and concavo-convex staggered Design of Screw Thread can remove the cumulus cell around ovocyte efficiently by retardance screening mode.
As shown in Figure 3, piping and druming pin is provided with handle 6, and piping and druming pin syringe needle 5 is equipped with in handle front portion, and the needle point of syringe needle is round and smooth, prevents from hurting ovocyte.Handle 6 is connected with air bag 8 by tracheae 7.
Syringe needle 5 diameter of piping and druming pin is set as 120 μm ~ 160 μm, object is in piping and druming cumulus oocyte mixture process, cumulus oocyte mixture can be covered completely blow and beat again, prevent cumulus oocyte mixture in the middle of piping and druming process to be blown to elsewhere.
Air bag 8 jams on contracting air-generating for pin.
Tracheae 7 comprises skin and internal layer, and skin is protective layer, and internal layer is induction pipe, and the length of tracheae is 1.5 ~ 2m.
Before piping and druming pin uses, first with piping and druming pin inspiration one section of liquid, object keeps cumulus oocyte mixture to remain in liquid in by piping and druming process, reduces damage.
Cleaning tool is as shown in Figure 4 made of plastic.Cleaning tool top is handle 11, and bottom is piston 9, and handle is connected with in the middle of piston.
Piston diameter is 66 ~ 68mm, with suitable with the internal diameter of small opening ware, just a little firmly just can fill in the inner chamber of small opening ware 1, to scrub.Be provided with multi-layer groove 10 at piston periphery, the effect of groove is the contact surface in order to reduce piston ridge and small opening ware, to reduce friction, and multi-layer groove there is little contact layer can play and better scrub effect.
Adopt said apparatus to remove the method for cumulus cell, comprise the following steps:
(1) in small opening ware, add nutrient solution, moved in small opening ware by washed cumulus oocyte mixture, rock small opening ware all around, object makes cumulus oocyte mixture be uniformly distributed;
(2) will be placed in above culture dish 12 that a diameter is 60mm at the bottom of the ware moving into the small opening ware of cumulus oocyte mixture, allow the circular pit at the bottom of small opening ware 1 ware just in time be stuck in the upper edge of culture dish 12, then add in 60mm culture dish 12 and operate liquid in right amount;
(3) under cumulus oocyte mixture being put into microscope, the handle of hand-held piping and druming pin, pin steps on air bag, first one section of liquid is sucked, then find object cumulus oocyte mixture under the microscope, pin and object cumulus oocyte mixture will be blown and beaten, small opening three alignment at the bottom of small opening ware ware, jam on air bag, object cumulus oocyte mixture is blown into and removes ovocyte small opening by piping and druming pin, the scraping retardation of concavo-convex staggered screw thread on small opening inwall, cumulus cell is separated with ovocyte, finally fall into and the 60mm culture dish be connected bottom small opening ware, carry out subsequent operations,
(4) choose the ovocyte after having removed cumulus cell and move into nutrient solution cultivation;
(5) outwelled by the drop in small opening ware, add distilled water and to case preservation with after Cleaning tool cleaning, used time alcohol washes, dries and can use.
Above-described embodiment of the present invention, does not form limiting the scope of the present invention.Any amendment done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within claims of the present invention.
Claims (10)
1. remove a cumulus cell device, it is characterized in that: comprise small opening ware, piping and druming pin, air bag;
Described small opening ware is circular, the uniform small opening removing cumulus cell at the bottom of ware, and described small opening diameter is 90 μm ~ 150 μm, is provided with internal thread in small opening;
Described piping and druming pin is provided with handle, and the needle point of piping and druming pin is round and smooth, and piping and druming pin is connected with air bag by tracheae.
2. according to claim 1ly remove cumulus cell device, it is characterized in that: described small opening diameter is respectively 150 μm, 120 μm, 90 μm, the small opening of 3 kinds of different diameters is uniformly distributed by diameter from the center of circle at the bottom of circular ware from big to small from inside to outside along radius.
3. according to claim 1ly removing cumulus cell device, it is characterized in that: at the bottom of the ware of described small opening ware, be provided with the circular pit for connecting culture dish.
4. according to claim 3ly remove cumulus cell device, it is characterized in that: the diameter of described small opening ware is 66mm, the circular pit diameter at the bottom of ware is 60mm, connects 60mm culture dish to facilitate.
5. according to claim 1ly remove cumulus cell device, it is characterized in that: the tip diameter of described piping and druming pin is set as 120 μm ~ 160 μm.
6. according to claim 1ly remove cumulus cell device, it is characterized in that: described air bag is that pin jams on contracting air-generating.
7. according to claim 1ly remove cumulus cell device, it is characterized in that: described tracheae comprises skin and internal layer, skin is protective layer, and internal layer is induction pipe, and the length of tracheae is 1.5 ~ 2m.
8. according to claim 1ly remove cumulus cell device, it is characterized in that: also comprise Cleaning tool made of plastic; Described Cleaning tool top is handle, and bottom is piston, and handle is connected with in the middle of piston; The internal diameter of piston diameter and small opening ware is suitable, and piston ridge is provided with multi-layer groove.
9. according to claim 1ly remove cumulus cell device, it is characterized in that: described small opening ware is that glass is made.
10. adopt according to claim 1 remove cumulus cell device remove cumulus cell method, it is characterized in that comprising the following steps:
(1) in small opening ware, add nutrient solution, moved in small opening ware by washed cumulus oocyte mixture, rock small opening ware all around, object makes cumulus oocyte mixture be uniformly distributed;
(2) annular recesses at the bottom of the small opening ware ware of immigration cumulus oocyte mixture is placed in above a culture dish, adds in culture dish and operate liquid in right amount;
(3) under cumulus oocyte mixture being put into microscope, the handle of hand-held piping and druming pin, pin steps on air bag, first one section of liquid is sucked, then find object cumulus oocyte mixture under the microscope, pin and object cumulus oocyte mixture will be blown and beaten, small opening three alignment at the bottom of small opening ware ware, jam on air bag, object cumulus oocyte mixture is blown into the small opening removing cumulus cell by piping and druming pin, the scraping retardation of concavo-convex staggered screw thread on small opening inwall, the cumulus cell be attached to around ovocyte is come off and is separated around ovocyte, ovocyte after separation and part cumulus cell finally fall into and the culture dish be connected bottom small opening ware, carry out subsequent operations,
(4) choose the immigration of the ovocyte after having removed cumulus cell nutrient solution to cultivate;
(5) drop in small opening ware is outwelled, add distilled water and clean for subsequent use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510658335.7A CN105199941B (en) | 2015-10-10 | 2015-10-10 | One kind removing cumulus cell device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510658335.7A CN105199941B (en) | 2015-10-10 | 2015-10-10 | One kind removing cumulus cell device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105199941A true CN105199941A (en) | 2015-12-30 |
| CN105199941B CN105199941B (en) | 2017-05-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510658335.7A Expired - Fee Related CN105199941B (en) | 2015-10-10 | 2015-10-10 | One kind removing cumulus cell device |
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| Country | Link |
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| CN (1) | CN105199941B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110184174A (en) * | 2019-07-12 | 2019-08-30 | 贵州省人民医院 | A kind of multipurpose cell and particle screen needle construction |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201150570Y (en) * | 2007-11-30 | 2008-11-19 | 许玉刚 | Mouth saccule type ovum peeling device |
| CN201309925Y (en) * | 2008-04-26 | 2009-09-16 | 刘雨生 | Granular cell dismantling tray used in external fertilization embryo transplantation technology |
| WO2010141320A1 (en) * | 2009-06-01 | 2010-12-09 | Mariposa Biotechnology, Inc. | Device for removing cumulus from oocytes |
| CN104055560A (en) * | 2014-07-06 | 2014-09-24 | 长沙傲图生物科技有限公司 | Electric ovum peeling device and method |
| CN204246224U (en) * | 2014-11-24 | 2015-04-08 | 遵义医学院 | A kind of novel stripping egg apparatus |
| CN204434608U (en) * | 2015-02-03 | 2015-07-01 | 中国农业科学院特产研究所 | A kind of ovocyte takes off ovarian cumulus pin |
-
2015
- 2015-10-10 CN CN201510658335.7A patent/CN105199941B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201150570Y (en) * | 2007-11-30 | 2008-11-19 | 许玉刚 | Mouth saccule type ovum peeling device |
| CN201309925Y (en) * | 2008-04-26 | 2009-09-16 | 刘雨生 | Granular cell dismantling tray used in external fertilization embryo transplantation technology |
| WO2010141320A1 (en) * | 2009-06-01 | 2010-12-09 | Mariposa Biotechnology, Inc. | Device for removing cumulus from oocytes |
| CN104055560A (en) * | 2014-07-06 | 2014-09-24 | 长沙傲图生物科技有限公司 | Electric ovum peeling device and method |
| CN204246224U (en) * | 2014-11-24 | 2015-04-08 | 遵义医学院 | A kind of novel stripping egg apparatus |
| CN204434608U (en) * | 2015-02-03 | 2015-07-01 | 中国农业科学院特产研究所 | A kind of ovocyte takes off ovarian cumulus pin |
Non-Patent Citations (1)
| Title |
|---|
| 北京兆迪科技有限公司: "《UG NX 9.0数控加工完全学习手册》", 30 June 2014, 机械工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110184174A (en) * | 2019-07-12 | 2019-08-30 | 贵州省人民医院 | A kind of multipurpose cell and particle screen needle construction |
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| Publication number | Publication date |
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| CN105199941B (en) | 2017-05-31 |
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Granted publication date: 20170531 Termination date: 20181010 |