CN105191897A - Capture separation method for soil animal - Google Patents
Capture separation method for soil animal Download PDFInfo
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- CN105191897A CN105191897A CN201510628833.7A CN201510628833A CN105191897A CN 105191897 A CN105191897 A CN 105191897A CN 201510628833 A CN201510628833 A CN 201510628833A CN 105191897 A CN105191897 A CN 105191897A
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- gauze
- container
- soil
- soil animal
- plastic cup
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Abstract
Disclosed is a capture separation method for a soil animal. The capture separation method for the soil animal comprises the steps that firstly, the surface soil animal is captured; secondly, a container is prepared, and clean water is placed into the container; thirdly, a mixed solution with the animal is poured on to gauze or a screen net above the container, and water or the solution in the mixed solution is filtered away; fourthly, a sample containing the gauze or the screen net is repeatedly washed and filtered in the clean water, and soil particles and impurities are filtered away; fifthly, tweezers are used for picking out leaves and large impurities inside the sample on the gauze; sixthly, the sample inside the gauze is guided into the specimen container; seventhly, the gauze is used for blocking a bottle opening of the specimen container, the bottle opening is slightly inclined, and clean water inside the container is filtered out; eighthly, alcohol or preservation liquid is guided into the specimen container. The capture separation method is used for capturing and separating the soil animal.
Description
Technical field
what the present invention relates to a kind of soil animal catches separating method.
Background technology
soil animal is movable at different levels in the middle of belowground ecosystem, and from earth's surface to underground, different according to its life habit, the separating method of catching of employing also has difference.
trap method is mainly used to catch the movable soil macro-invertebrate on earth's surface, in actual acquisition process, is subject to the impact such as wind, precipitation, usually all can infiltrates some impurity in plastic cup.The impurity such as these leaves, branch, grogs and earth's surface soil macro-invertebrate mix.Sorting comprises hand and picks up method, uses tweezers etc. directly to pick out from plastic cup by soil macro-invertebrate sample.But this can bring following problem: 1) speed is slow, inefficiency; 2) time with tool holder samples such as tweezers, can damage sample, as folder falls wing, the feeler of sample, some animal is as julid, body segment is a lot, if can break as a lot of joint with tweezers folder, destructiveness is very large, for the Specimen identification in later stage and the acquisition of parameter add very large difficulty; 3) have the risk of omitting sample, insect is mixed in inside leaf and grogs, and comparatively dark, that build is the relatively little animal naked eyes of some color are often difficult to differentiate, and are not easy in the wild to pick totally, reduce the accuracy of sample investigation.
Summary of the invention
the object of this invention is to provide a kind of be separated the soil animal clean, Saving specimen is complete catch separating method.
above-mentioned object is realized by following technical scheme:
soil animal catch a separating method, this method comprises eight steps, and the first step catches earth's surface soil animal; Second step prepares container, puts into clean clear water in container; Mixed solution with animal, above this container, is poured on gauze or screen cloth by the 3rd step, filters water or the solution of the inside; The sample of splendid attire gauze or screen cloth is repeatedly eluriated, is filtered by the 4th step in clean clear water, filters grogs, impurity; Leaf in sample on gauze, bulk impurity are picked out by the 5th step tweezers; 6th step is by the Sample introduction specimen container in gauze; The bottleneck of specimen container blocked by 7th step gauze, and inclination bottleneck gently, filters out the clear water of the inside; 8th step imports alcohol or conserving liquid in specimen container.
described soil animal catch separating method, the described first step catches soil animal, catch the movable soil macro-invertebrate on earth's surface, first arrange plastic cup, in soil, dig a hole with earth boring auger, then plastic cup is placed in hole, liquid is placed in plastic cup, rim of a cup flushes with ground, and the soil animal that all movements are passed by drops in plastic cup, kills and preserve these zoological specimens; Above plastic cup, prop the lid of a plastic cup with pole, distance rim of a cup 5-10cm, prevents entering of impurity.
described soil animal catch separating method, the diameter of described second step container is 30-32cm; The height of described clear water is 5-10cm.
described soil animal catch separating method, described gauze, described screen cloth 8-10cm, the periphery of described gauze, described screen cloth connects extension limit.
beneficial effect:
1. the surface soil animal that catches of the present invention, can reflect the essential characteristic of surface soil animal community, simple to operate, makes a collection of specimens complete.
the surface soil animal that the present invention is separated is totally complete, and convenient preservation, is convenient to follow-up study.
the present invention is separated soil animal and saves time, and efficiency is high.
the present invention is separated soil animal convenient operation, does not have special requirement to indoor and outdoors condition of work.
embodiment:
embodiment 1:
soil animal catch a separating method, this method comprises eight steps, and the first step catches earth's surface soil animal; Second step prepares container, puts into clean clear water in container; Mixed solution with animal, above this container, is poured on gauze or screen cloth by the 3rd step, filters water or the solution of the inside; The sample of splendid attire gauze or screen cloth is repeatedly eluriated, is filtered by the 4th step in clean clear water, filters grogs, impurity; Leaf in sample on gauze, bulk impurity are picked out by the 5th step tweezers; 6th step is by the Sample introduction specimen container in gauze; The bottleneck of specimen container blocked by 7th step gauze, and inclination bottleneck gently, filters out the clear water of the inside; 8th step imports alcohol or conserving liquid in specimen container.
embodiment 2:
soil animal described in embodiment 1 catch separating method, the described first step catches soil animal, catch the movable soil macro-invertebrate on earth's surface as ground beetle, spider etc., first plastic cup is arranged, in soil, a hole is dug with earth boring auger, then plastic cup is placed in hole, the built-in fixing liquid of plastic cup is as saturated NaCl solution or 4% formalin, ensure that rim of a cup flushes with ground, the soil animal that all movements are passed by can be random drop in plastic cup, and effectively kill and preserve these zoological specimens; Usual meeting above plastic cup with pole as chopsticks or branch prop the lid of a plastic cup as disposable plastic service plate or Polypropylence Sheet etc., distance rim of a cup 5-10cm, prevents entering as leaf, branch, soil particle etc. of impurity.
embodiment 3:
soil animal described in embodiment 1 catch separating method, the diameter of described second step container is 30-32cm; The height of described clear water is 5-10cm.
embodiment 4:
soil animal described in embodiment 1 catch separating method, described gauze, described screen cloth 8-10cm, the periphery of described gauze, described screen cloth connects extension limit.
embodiment 5:
soil animal described in above-described embodiment catch separating method, second step prepares container, clean clear water is put in container, first the container that preparation one is enough large is as plastic wash-basin, and specification has 30cm or 32cm etc., built-in clear water, the height 5-10cm of clear water, mixed solution with animal, above this container, is poured on gauze or screen cloth by the 3rd step, filters water or the solution of the inside; Prepare the circular screen mesh that a screen cloth radius with suitable vessel mouth size is 8-10cm, plastics soft on doing around circular screen mesh or gauze etc., facilitate two hand-held.These plastics not the whole of circle are closed, and two heads are not to connecting, and stay an osculum conveniently can pour sample, the sample of splendid attire gauze or screen cloth is repeatedly eluriated, filtered by the 4th step in clean clear water, filters grogs, impurity; The upper vessel portion of clear water is being housed, the mixed solution in plastic cup is being poured on gauze and filters, repeatedly rinse plastic cup with clear water, till cleaning up and ensureing that all samples all pour out.Then two hand-held gauzes, are immersed in gauze in the clear water of container, repeatedly eluriate 5-10 time, necessarily note in this process, and the sample on gauze must not be allowed to topple in container, and this understands bring very large trouble to work afterwards.After eluriating several times, the bulk grogs, gravel etc. of plastic cup sample mix all can spill, and the leaf in sample on gauze, bulk impurity are picked out by the 5th step tweezers; Then with tweezers, some bulks on gauze top and obvious leaf, branch etc. are picked out.Sample is at this time just very clean, and the 6th step is by the Sample introduction specimen container in gauze; Then gauze is rolled into a rectangular shape, by the sample specimen container pouring splendid attire soil animal into gently as bottle or spool box etc.But some clear water can be entered into like this, the concentration of meeting dilute alcohol.So next, the bottleneck of specimen container blocked by the 7th step gauze, and inclination bottleneck gently, filters out the clear water of the inside; Block the bottleneck of specimen container again with gauze, inclination bottleneck gently, all pours out the clear water in specimen container, and the 8th step imports alcohol or conserving liquid in specimen container; And then put the solution of the preservation such as alcohol or saturated NaCl.So far, the trap method separation of a sample of soil macro-invertebrate of catching and work of picking just finish.
embodiment 6:
soil animal described in embodiment 2 catch separating method, the described first step catches soil animal, catch the movable soil macro-invertebrate on earth's surface as ground beetle, spider etc., first plastic cup is arranged, in soil, a hole is dug with earth boring auger, then by plastic cup as plastic cup, containers etc. are placed in hole, the built-in fixing liquid of plastic cup is as saturated NaCl solution or 4% formalin, ensure that rim of a cup flushes with ground, the soil animal that all movements are passed by can be random drop in plastic cup, and effectively kill and preserve these zoological specimens, usual meeting is chopsticks or branch with pole above plastic cup, props the lid of a plastic cup as disposable plastic service plate or Polypropylence Sheet etc., and distance rim of a cup 5cm, prevents entering as leaf, branch, soil particle etc. of impurity.
embodiment 7:
soil animal described in embodiment 2 catch separating method, the described first step catches soil animal, catch the movable soil macro-invertebrate on earth's surface as ground beetle, spider etc., first plastic cup is arranged, in soil, a hole is dug with earth boring auger, then by plastic cup as plastic cup, containers etc. are placed in hole, the built-in fixing liquid of plastic cup is as saturated NaCl solution or 4% formalin, ensure that rim of a cup flushes with ground, the soil animal that all movements are passed by can be random drop in plastic cup, and effectively kill and preserve these zoological specimens, usual meeting is chopsticks or branch with pole above plastic cup, and the lid propping a plastic cup, as disposable plastic service plate or the equidistant rim of a cup 6cm of Polypropylence Sheet, prevents entering as leaf, branch, soil particle etc. of impurity.
embodiment 8:
soil animal described in embodiment 2 catch separating method, the described first step catches soil animal, catch the movable soil macro-invertebrate on earth's surface as ground beetle, spider etc., first plastic cup is arranged, in soil, a hole is dug with earth boring auger, then by plastic cup as plastic cup, containers etc. are placed in hole, the built-in fixing liquid of plastic cup is as saturated NaCl solution or 4% formalin, ensure that rim of a cup flushes with ground, the soil animal that all movements are passed by can be random drop in plastic cup, and effectively kill and preserve these zoological specimens, usual meeting props the lid of a plastic cup as disposable plastic service plate or Polypropylence Sheet etc. above plastic cup with pole, distance rim of a cup 7cm, prevents entering as leaf, branch, soil particle etc. of impurity.
embodiment 9:
soil animal described in embodiment 3 catch separating method, the diameter of described second step container is 30cm; The height of described clear water is 5cm.
embodiment 10:
soil animal described in embodiment 3 catch separating method, the diameter of described second step container is 32cm; The height of described clear water is 10m.
embodiment 11:
soil animal described in embodiment 3 catch separating method, the diameter of described second step container is 31cm; The height of described clear water is 8cm.
Claims (4)
1. soil animal catch a separating method, it is characterized in that: this method comprises eight steps, the first step catches earth's surface soil animal; Second step prepares container, puts into clean clear water in container; Mixed solution with animal, above this container, is poured on gauze or screen cloth by the 3rd step, filters water or the solution of the inside; The sample of splendid attire gauze or screen cloth is repeatedly eluriated, is filtered by the 4th step in clean clear water, filters grogs, impurity; Leaf in sample on gauze, bulk impurity are picked out by the 5th step tweezers; 6th step is by the Sample introduction specimen container in gauze; The bottleneck of specimen container blocked by 7th step gauze, and inclination bottleneck gently, filters out the clear water of the inside; 8th step imports alcohol or conserving liquid in specimen container.
2. soil animal according to claim 1 catch separating method, it is characterized in that: the described first step catches earth's surface soil animal, catch the movable soil macro-invertebrate on earth's surface, first arrange plastic cup, in soil, dig a hole with earth boring auger, then plastic cup is placed in hole, liquid is placed in plastic cup, rim of a cup flushes with ground, and the soil animal that all movements are passed by drops in plastic cup, kills and preserve these zoological specimens; Above plastic cup, prop the lid of a plastic cup with pole, distance rim of a cup 5-10cm, prevents entering of impurity.
3. soil animal according to claim 1 catch separating method, it is characterized in that: the diameter of described second step container is 30-32cm; The height of described clear water is 5-10cm.
4. soil animal according to claim 1 catch separating method, it is characterized in that: described gauze, described screen cloth 8-10cm, the periphery of described gauze, described screen cloth connects extension limit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510628833.7A CN105191897A (en) | 2015-09-29 | 2015-09-29 | Capture separation method for soil animal |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510628833.7A CN105191897A (en) | 2015-09-29 | 2015-09-29 | Capture separation method for soil animal |
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| CN105191897A true CN105191897A (en) | 2015-12-30 |
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| CN201510628833.7A Pending CN105191897A (en) | 2015-09-29 | 2015-09-29 | Capture separation method for soil animal |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113740510A (en) * | 2021-09-06 | 2021-12-03 | 宁波大学 | Screening method of green ecological buoy of farmland soil animal activity biosensor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004201519A (en) * | 2002-12-24 | 2004-07-22 | Kansai Santec:Kk | Mothproofing/insect collecting lamp using luminescent color of light-emitting diode and controller therefor |
| CN203872859U (en) * | 2014-04-29 | 2014-10-15 | 云南农业大学 | Portable sugarcane root prionid trapper |
| CN104094898A (en) * | 2014-05-19 | 2014-10-15 | 中国科学院动物研究所 | Design and application of soil meso-and micro-invertebrate separation equipment based on farmland landscape and ecological function research |
| CN104597223A (en) * | 2015-01-12 | 2015-05-06 | 东北农业大学 | Method for evaluating soil quality by employing nematodes as biomarkers |
| CN104604824A (en) * | 2015-02-12 | 2015-05-13 | 中国林业科学研究院资源昆虫研究所 | Trapping device for ground arthropods |
-
2015
- 2015-09-29 CN CN201510628833.7A patent/CN105191897A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004201519A (en) * | 2002-12-24 | 2004-07-22 | Kansai Santec:Kk | Mothproofing/insect collecting lamp using luminescent color of light-emitting diode and controller therefor |
| CN203872859U (en) * | 2014-04-29 | 2014-10-15 | 云南农业大学 | Portable sugarcane root prionid trapper |
| CN104094898A (en) * | 2014-05-19 | 2014-10-15 | 中国科学院动物研究所 | Design and application of soil meso-and micro-invertebrate separation equipment based on farmland landscape and ecological function research |
| CN104597223A (en) * | 2015-01-12 | 2015-05-06 | 东北农业大学 | Method for evaluating soil quality by employing nematodes as biomarkers |
| CN104604824A (en) * | 2015-02-12 | 2015-05-13 | 中国林业科学研究院资源昆虫研究所 | Trapping device for ground arthropods |
Non-Patent Citations (1)
| Title |
|---|
| 李果等: "《生物多样性监测技术手册》", 31 May 2014, 中国环境出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113740510A (en) * | 2021-09-06 | 2021-12-03 | 宁波大学 | Screening method of green ecological buoy of farmland soil animal activity biosensor |
| CN113740510B (en) * | 2021-09-06 | 2023-09-08 | 宁波大学 | Method for screening green ecological buoy of farmland soil animal active biosensor |
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Application publication date: 20151230 |