CN105169460A - Magnetically therapeutic antibacterial haemostatic wound dressing and preparation method thereof - Google Patents
Magnetically therapeutic antibacterial haemostatic wound dressing and preparation method thereof Download PDFInfo
- Publication number
- CN105169460A CN105169460A CN201510699965.9A CN201510699965A CN105169460A CN 105169460 A CN105169460 A CN 105169460A CN 201510699965 A CN201510699965 A CN 201510699965A CN 105169460 A CN105169460 A CN 105169460A
- Authority
- CN
- China
- Prior art keywords
- solution
- dressing
- magnetic therapy
- antibacterial anti
- prepared
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 230000000025 haemostatic effect Effects 0.000 title abstract description 7
- 229940030225 antihemorrhagics Drugs 0.000 title abstract description 6
- 230000001225 therapeutic effect Effects 0.000 title abstract description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 41
- 229920001661 Chitosan Polymers 0.000 claims abstract description 28
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229940072056 alginate Drugs 0.000 claims abstract description 26
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 26
- 229920000615 alginic acid Polymers 0.000 claims abstract description 26
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 23
- 239000005543 nano-size silicon particle Substances 0.000 claims abstract description 23
- 239000003102 growth factor Substances 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 60
- 238000002653 magnetic therapy Methods 0.000 claims description 45
- 230000002364 anti-haemorrhagic effect Effects 0.000 claims description 41
- 239000002105 nanoparticle Substances 0.000 claims description 28
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims description 25
- 102000013275 Somatomedins Human genes 0.000 claims description 25
- 238000003756 stirring Methods 0.000 claims description 25
- 230000005291 magnetic effect Effects 0.000 claims description 24
- 239000000377 silicon dioxide Substances 0.000 claims description 17
- 235000012239 silicon dioxide Nutrition 0.000 claims description 16
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 239000002245 particle Substances 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 12
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000008213 purified water Substances 0.000 claims description 10
- 229910052710 silicon Inorganic materials 0.000 claims description 10
- 239000010703 silicon Substances 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000010261 cell growth Effects 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical group CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 229910052814 silicon oxide Inorganic materials 0.000 claims description 6
- 235000010413 sodium alginate Nutrition 0.000 claims description 6
- 239000000661 sodium alginate Substances 0.000 claims description 6
- 229940005550 sodium alginate Drugs 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical group [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 5
- 102000018233 Fibroblast Growth Factor Human genes 0.000 claims description 5
- 108050007372 Fibroblast Growth Factor Proteins 0.000 claims description 5
- BOTDANWDWHJENH-UHFFFAOYSA-N Tetraethyl orthosilicate Chemical compound CCO[Si](OCC)(OCC)OCC BOTDANWDWHJENH-UHFFFAOYSA-N 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 238000001354 calcination Methods 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 230000006196 deacetylation Effects 0.000 claims description 5
- 238000003381 deacetylation reaction Methods 0.000 claims description 5
- 230000005294 ferromagnetic effect Effects 0.000 claims description 5
- 229940126864 fibroblast growth factor Drugs 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims description 5
- 238000007885 magnetic separation Methods 0.000 claims description 5
- 230000005389 magnetism Effects 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 229910001172 neodymium magnet Inorganic materials 0.000 claims description 5
- 230000005855 radiation Effects 0.000 claims description 5
- 239000000376 reactant Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 238000005516 engineering process Methods 0.000 claims description 4
- 230000035876 healing Effects 0.000 abstract description 6
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 206010061218 Inflammation Diseases 0.000 abstract 1
- 230000000740 bleeding effect Effects 0.000 abstract 1
- 230000003467 diminishing effect Effects 0.000 abstract 1
- 230000004054 inflammatory process Effects 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 239000002122 magnetic nanoparticle Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 26
- 210000003491 skin Anatomy 0.000 description 17
- 206010052428 Wound Diseases 0.000 description 12
- 208000027418 Wounds and injury Diseases 0.000 description 12
- 230000029663 wound healing Effects 0.000 description 11
- 230000023597 hemostasis Effects 0.000 description 8
- 239000008280 blood Substances 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 210000004927 skin cell Anatomy 0.000 description 6
- 239000003814 drug Substances 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 210000000589 cicatrix Anatomy 0.000 description 4
- 230000004936 stimulating effect Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000024883 vasodilation Effects 0.000 description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 3
- 102000003886 Glycoproteins Human genes 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 3
- 206010020880 Hypertrophy Diseases 0.000 description 3
- 206010062575 Muscle contracture Diseases 0.000 description 3
- 230000000202 analgesic effect Effects 0.000 description 3
- 230000003110 anti-inflammatory effect Effects 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000019522 cellular metabolic process Effects 0.000 description 3
- 230000004087 circulation Effects 0.000 description 3
- 208000006111 contracture Diseases 0.000 description 3
- 210000004087 cornea Anatomy 0.000 description 3
- 229920002674 hyaluronan Polymers 0.000 description 3
- 229960003160 hyaluronic acid Drugs 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000004005 microsphere Substances 0.000 description 3
- 210000004877 mucosa Anatomy 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000036407 pain Effects 0.000 description 3
- 231100000241 scar Toxicity 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 229910001424 calcium ion Inorganic materials 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 208000010392 Bone Fractures Diseases 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
Abstract
The invention belongs to the field of medicinal dressings, and particularly relates to a magnetically therapeutic antibacterial haemostatic wound dressing and a preparation method thereof. The magnetically therapeutic antibacterial haemostatic wound dressing is prepared from the following components in parts by weight: 0.5-4 parts of alginate, 0.5-6 parts of chitosan, 0.5-4 parts of a crosslinking agent, 0.1-2 parts of permanent magnetic nano-particles, 0.5-4 parts of nano-silicon dioxide and 0.005-0.5 part of growth factors. The magnetically therapeutic antibacterial haemostatic wound dressing can be used for quickly stopping bleeding, inhibiting bacteria, diminishing inflammation and promoting healing of a wound, is simple in preparation process, and is good in stability and convenient to carry.
Description
Technical field
The invention belongs to medical dressing field, be specifically related to a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof.
Background technology
Skin energy long term storage electric charge or maintenance polarized state, the action of a magnetic field affects the CURRENT DISTRIBUTION of human body in tissue, the motion of Charge particle, the permeability of membranous system and the equimolecular magnetic moment orientation effect of biology, histiocytic physiology, biochemical process are changed, cause cutaneous vasodilation and blood circulation to increase, play the effect of blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, promotion wound healing.Current electrostatic or magnetic therapy great majority are used for the symptom such as set a broken bone, joint, fewer for clinical wound care.
Chitosan, alginate etc. are natural active biomaterials, not only have good biocompatibility, degradability, and have antibacterial, hemostasis, promote the effect such as wound healing.Nano silicon not only has antibacterial activity, and has adsorption function, is a kind of good medical material.Somatomedin is the active factors that a class has stimulating cellular growth, growth, can promote the healing of skin and mucosa wound face, reduces scar contracture and skin deformity hypertrophy, accelerates the reparation of the superficial wound such as cornea, skin.In addition, somatomedin can also promote that Skin Cell is to the absorption of nutrient substance, accelerates the metabolism of cell, promotes division and the growth of Skin Cell, promotes the synthesis of hyaluronic acid and glycoprotein, play the effect of skin care.
Be used for treating wound surface, hemostasis and pain-relieving, promotion wound healing, the conbined usage that suppresses the product of cicatrix to be single functional dressings or dressing and medicine mostly in clinical at present.Single functional dressings compared with traditional dressing, have certain hemostasis, antibacterial, promote the function such as wound healing, but effect is effective.Although medicine effectiveness comparison is remarkable, medicine easily makes people produce side reaction, affects healthy, and the absorption of skin surface to medicine is limited, greatly reduces the effect of medicine.The material with magnetic therapy functions is added in functional dressing, the curative effect of physical curative effect and biological dressing is joined together, be used for treating wound surface, hemostasis and pain-relieving, promotion wound healing, suppress cicatrix, antibacterial etc. research has no report at present.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, the material with magnetic therapy functions adds in functional dressing by the magnetic therapy antibacterial anti hemorrhagic dressing using the method to prepare, the curative effect of physical curative effect and biological dressing is joined together, treatment wound surface, hemostasis and pain-relieving, promotion wound healing, suppress cicatrix, antibacterial etc., increased the circulation of cutaneous vasodilation and blood by the magnetotherapy effect of this dressing, play anti-inflammatory analgetic, promote the effect of wound healing; Can stimulating cellular growth, growth, reduce cicatrix, repair damaged cell, soft skin; Have simultaneously absorb oozing of blood, antibacterial, hemostasis effect.
The object of this invention is to provide a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, in this magnetic therapy antibacterial anti hemorrhagic dressing, chitosan and sodium alginate cross-linking reaction can form three-dimensional space net structure, by magnetic mesoporous Silica-coated wherein, then, under the effect of cross-linking agent, microparticle is formed.
Technical scheme of the present invention is as follows: the dressing of a kind of magnetic therapy antibacterial anti hemorrhagic, counts by weight and is made up of following composition:
Alginate 0.5 ~ 4 part;
Chitosan 0.5 ~ 6 part;
Cross-linking agent 0.5 ~ 4 part;
Permanent magnet nanoparticle 0.1 ~ 2 part;
Nano silicon 0.5 ~ 4 part;
Somatomedin 0.005 ~ 0.5 part.
Further, described alginate is sodium alginate.
Further, the deacetylation of described chitosan is greater than 95%, and molecular mass is 10000 ~ 20000Da.This chitosan has good antibiotic property and absorbable and degradable.
Preferably, described cross-linking agent is calcium chloride.Can by alginate, chitosan and magnetic mesoporous silicon dioxide embedded, be prepared into microsphere particle, this granule can absorb oozing of blood sepage fast, plays the effect of hemostasis, antibacterial, pain relieving, promotion wound healing.
Further, the ferromagnetic nanoparticle of described permanent magnetism is Nd-Fe-B magnetite particle, and magnetic field intensity is 0.15 ~ 0.35 tesla.
Preferably, the aperture of described nano silicon is 20 ~ 80 μm.Not only can adsorb permanent magnet nanoparticle, prepare magnetic mesoporous silicon dioxide, be increased the circulation of cutaneous vasodilation and blood by magnetotherapy effect, play anti-inflammatory analgetic, promote the effect of wound healing; And a large amount of calcium ion can be adsorbed, play the effect of quick-acting haemostatic powder.
Further, described somatomedin is fibroblast growth factor or epithelical cell growth factor.Can stimulating cellular growth, growth, promote the healing of skin and mucosa wound surface, reduce scar contracture and skin deformity hypertrophy, accelerate the reparation of the superficial wound such as cornea, skin.Can also promote that Skin Cell is to the absorption of nutrient substance simultaneously, accelerate the metabolism of cell, promote division and the growth of Skin Cell, promote the synthesis of hyaluronic acid and glycoprotein, play the effect of skin care.
Further, described a kind of magnetic therapy antibacterial anti hemorrhagic dressing preparation method concrete technology step is as follows:
S1. alginate is dissolved in purified water, mixes and stir 0.5h ~ 1h to evenly, being prepared into solution A; Chitosan being dissolved in mass fraction is in the acetic acid solution of 1%, mixes and stirs 0.5h ~ 1h to evenly, being prepared into solution B;
S2. the solution A obtained in S1 slowly added in solution B, mixing also stirring reaction 0.5h ~ 1h, to evenly, is prepared into solution C;
S3. by permanent magnet nanoparticle, the mass fraction be directly distributed to containing nano silicon is carry out the embedding of inorganic silicon source in the sodium hydroxide solution of 8%, the permanent magnet nanoparticle completing the embedding of inorganic silicon source is distributed in aqueous solution, adding cetyl trimethyl ammonium bromide template and ethyl orthosilicate again, to carry out mesoporous silicon oxide coated, after reactant being carried out magnetic separation, washing, the dry removal of impurity, in 500 DEG C of calcinings 5 ~ 7 hours, form magnetic mesoporous silicon dioxide, magnetic mesoporous silicon dioxide is slowly added in solution C obtained in S2, be prepared into solution D;
S4. cross-linking agent is dissolved in purified water, is prepared into solution E, solution D is slowly sprayed in solution E, more spray-dried, being prepared into particle diameter is 100 ~ 200 object flour F;
S5. somatomedin is slowly added in material F, stir, after stirring 0.5h ~ 1h, be mixed into solid powder G;
S6. by solid powder G filling and sealing and outer package, and then carry out radiation sterilization and namely obtain described magnetic therapy antibacterial anti hemorrhagic dressing.
Further, magnetic mesoporous silicon dioxide is distributed in chitosan alginate soln and is prepared into the stability that microsphere particle can improve product again by described S3, S4, prevents magnetisable material in the process of subpackage, sterilizing from being adsorbed by the object containing metal.
Further, described S5 can prevent somatomedin from not affecting by High Temperature High Pressure, keeps its high activity.
Beneficial effect of the present invention is: the magnetic therapy antibacterial anti hemorrhagic dressing that (1) the present invention adopts the method to prepare is by alginate, chitosan and magnetic mesoporous silicon dioxide embedded, be prepared into microsphere particle, this granule can absorb oozing of blood sepage fast, plays the effect of hemostasis, antibacterial, pain relieving, promotion wound healing.(2) mesoporous silicon oxide adsorbed permanent magnet nanoparticle, prepare magnetic mesoporous silicon dioxide, not only can be increased the circulation of cutaneous vasodilation and blood by magnetotherapy effect, play anti-inflammatory analgetic, promote the effect of wound healing; And magnetic mesoporous silicon dioxide can also adsorb a large amount of calcium ion, play the effect of quick-acting haemostatic powder.(3) somatomedin can stimulating cellular growth, growth, promote the healing of skin and mucosa wound surface, reduce scar contracture and skin deformity hypertrophy, accelerate the reparation of the superficial wound such as cornea, skin.Can also promote that Skin Cell is to the absorption of nutrient substance simultaneously, accelerate the metabolism of cell, promote division and the growth of Skin Cell, promote the synthesis of hyaluronic acid and glycoprotein, play the effect of skin care.(4) preparation technology of the present invention does not destroy the active matter function in product, does not affect the stability of product quality, by magnetic therapy and functional dressings conbined usage, can significantly improve therapeutic effect.
Detailed description of the invention
Below in conjunction with embodiment, technical scheme of the present invention is described further.
embodiment 1
The dressing of a kind of magnetic therapy antibacterial anti hemorrhagic, counts by weight and is made up of following composition: alginate 0.5 part, chitosan 0.5 part, cross-linking agent 0.5 part, permanent magnet nanoparticle 0.1 part, nano silicon 0.5 part, somatomedin 0.005 part.Described alginate is sodium alginate, and described cross-linking agent is calcium chloride, and the deacetylation of described chitosan is greater than 95%, and molecular mass is 10000 ~ 20000Da; The ferromagnetic nanoparticle of described permanent magnetism is Nd-Fe-B magnetite particle, and magnetic field intensity is 0.15 ~ 0.35 tesla; The aperture of described nano silicon is 20 ~ 80 μm; Described somatomedin is fibroblast growth factor or epithelical cell growth factor.
A preparation method for magnetic therapy antibacterial anti hemorrhagic dressing, comprises the steps:
S1. alginate is dissolved in purified water, mixes and stir 0.5h ~ 1h to evenly, being prepared into solution A; Chitosan being dissolved in mass fraction is in the acetic acid solution of 1%, mixes and stirs 0.5h ~ 1h to evenly, being prepared into solution B;
S2. the solution A obtained in S1 slowly added in solution B, mixing also stirring reaction 0.5h ~ 1h, to evenly, is prepared into solution C;
S3. by permanent magnet nanoparticle, the mass fraction be directly distributed to containing nano silicon is carry out the embedding of inorganic silicon source in the sodium hydroxide solution of 8%, the permanent magnet nanoparticle completing the embedding of inorganic silicon source is distributed in aqueous solution, adding cetyl trimethyl ammonium bromide template and ethyl orthosilicate again, to carry out mesoporous silicon oxide coated, after reactant being carried out magnetic separation, washing, the dry removal of impurity, in 500 DEG C of calcinings 5 ~ 7 hours, form magnetic mesoporous silicon dioxide, magnetic mesoporous silicon dioxide is slowly added in solution C obtained in S2, be prepared into solution D;
S4. cross-linking agent is dissolved in purified water, is prepared into solution E, solution D is slowly sprayed in solution E, more spray-dried, being prepared into particle diameter is 100 ~ 200 object flour F;
S5. somatomedin is slowly added in material F, stir, after stirring 0.5h ~ 1h, be mixed into solid powder G;
S6. by solid powder G filling and sealing and outer package, and then carry out radiation sterilization and obtain described magnetic therapy antibacterial anti hemorrhagic dressing.
embodiment 2
A kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, counts by weight and is made up of following composition: alginate 2 parts, chitosan 3 parts, cross-linking agent 2 parts, permanent magnet nanoparticle 1 part, nano silicon 2 parts, somatomedin 0.2 part.Described alginate is sodium alginate, and described cross-linking agent is calcium chloride, and the deacetylation of described chitosan is greater than 95%, and molecular mass is 10000 ~ 20000Da; The ferromagnetic nanoparticle of described permanent magnetism is Nd-Fe-B magnetite particle, and magnetic field intensity is 0.15 ~ 0.35 tesla; The aperture of described nano silicon is 20 ~ 80 μm; Described somatomedin is fibroblast growth factor or epithelical cell growth factor.
A preparation method for magnetic therapy antibacterial anti hemorrhagic dressing, comprises the steps:
S1. alginate is dissolved in purified water, mixes and stir 0.5h ~ 1h to evenly, being prepared into solution A; Chitosan being dissolved in mass fraction is in the acetic acid solution of 1%, mixes and stirs 0.5h ~ 1h to evenly, being prepared into solution B;
S2. the solution A obtained in S1 slowly added in solution B, mixing also stirring reaction 0.5h ~ 1h, to evenly, is prepared into solution C;
S3. by permanent magnet nanoparticle, the mass fraction be directly distributed to containing nano silicon is carry out the embedding of inorganic silicon source in the sodium hydroxide solution of 8%, the permanent magnet nanoparticle completing the embedding of inorganic silicon source is distributed in aqueous solution, adding cetyl trimethyl ammonium bromide template and ethyl orthosilicate again, to carry out mesoporous silicon oxide coated, after reactant being carried out magnetic separation, washing, the dry removal of impurity, in 500 DEG C of calcinings 5 ~ 7 hours, form magnetic mesoporous silicon dioxide, magnetic mesoporous silicon dioxide is slowly added in solution C obtained in S2, be prepared into solution D;
S4. cross-linking agent is dissolved in purified water, is prepared into solution E, solution D is slowly sprayed in solution E, more spray-dried, being prepared into particle diameter is 100 ~ 200 object flour F;
S5. somatomedin is slowly added in material F, stir, after stirring 0.5h ~ 1h, be mixed into solid powder G;
S6. by solid powder G filling and sealing and outer package, and then carry out radiation sterilization and obtain described magnetic therapy antibacterial anti hemorrhagic dressing.
embodiment 3
A kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, counts by weight and is made up of following composition: alginate 4 parts, chitosan 6 parts, cross-linking agent 4 parts, permanent magnet nanoparticle 2 parts, nano silicon 4 parts, somatomedin 0.5 part.Described alginate is sodium alginate, and described cross-linking agent is calcium chloride, and the deacetylation of described chitosan is greater than 95%, and molecular mass is 10000 ~ 20000Da; The ferromagnetic nanoparticle of described permanent magnetism is Nd-Fe-B magnetite particle, and magnetic field intensity is 0.15 ~ 0.35 tesla; The aperture of described nano silicon is 20 ~ 80 μm; Described somatomedin is fibroblast growth factor or epithelical cell growth factor.
A preparation method for magnetic therapy antibacterial anti hemorrhagic dressing, comprises the steps:
S1. alginate is dissolved in purified water, mixes and stir 0.5h ~ 1h to evenly, being prepared into solution A; Chitosan being dissolved in mass fraction is in the acetic acid solution of 1%, mixes and stirs 0.5h ~ 1h to evenly, being prepared into solution B;
S2. the solution A obtained in S1 slowly added in solution B, mixing also stirring reaction 0.5h ~ 1h, to evenly, is prepared into solution C;
S3. by permanent magnet nanoparticle, the mass fraction be directly distributed to containing nano silicon is carry out the embedding of inorganic silicon source in the sodium hydroxide solution of 8%, the permanent magnet nanoparticle completing the embedding of inorganic silicon source is distributed in aqueous solution, adding cetyl trimethyl ammonium bromide template and ethyl orthosilicate again, to carry out mesoporous silicon oxide coated, after reactant being carried out magnetic separation, washing, the dry removal of impurity, in 500 DEG C of calcinings 5 ~ 7 hours, form magnetic mesoporous silicon dioxide, magnetic mesoporous silicon dioxide is slowly added in solution C obtained in S2, be prepared into solution D;
S4. cross-linking agent is dissolved in purified water, is prepared into solution E, solution D is slowly sprayed in solution E, more spray-dried, being prepared into particle diameter is 100 ~ 200 object flour F;
S5. somatomedin is slowly added in material F, stir, after stirring 0.5h ~ 1h, be mixed into solid powder G;
S6. by solid powder G filling and sealing and outer package, and then carry out radiation sterilization and obtain described magnetic therapy antibacterial anti hemorrhagic dressing.
For whether the dressing of checking magnetic therapy antibacterial anti hemorrhagic of the present invention reaches the invention effect of expection, carry out antibacterial tests and animal experiment analysis to magnetic therapy antibacterial anti hemorrhagic of the present invention dressing, result is as follows:
Cause the pathogenic bacterium of gynecological and traumatic infection mainly to comprise staphylococcus aureus, bacillus subtilis, coliform, Hemolytic streptococcus etc., this experiment is with the antibacterial effect of above-mentioned pathogenic bacterium testing example 1 ~ 3 magnetic therapy antibacterial anti hemorrhagic dressing.As can be seen from the above table, magnetic therapy antibacterial anti hemorrhagic dressing prepared by the present invention has good antibacterial effect to most of pathogenic bacterium, there is good haemostatic effect, biocompatibility simultaneously, and safety non-toxic, can use safely, the resistance of skin can be improved, promote the metabolic capabilities of skin, aseptic healing environment can be provided to wound, promote the healing of wound.
embodiment 4
For checking magnetic therapy antibacterial anti hemorrhagic of the present invention dressing superiority on formula, the present invention tests as follows.
Experimental group: a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, counts by weight and is made up of following composition: alginate 2 parts, chitosan 3 parts, cross-linking agent 2 parts, permanent magnet nanoparticle 1 part, nano silicon 2 parts, somatomedin 0.2 part.
Matched group 1: a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, counts by weight and is made up of following composition: chitosan 3 parts, cross-linking agent 2 parts, permanent magnet nanoparticle 1 part, nano silicon 2 parts, somatomedin 0.2 part.
Matched group 2: a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, counts by weight and is made up of following composition: alginate 2 parts, cross-linking agent 2 parts, permanent magnet nanoparticle 1 part, nano silicon 2 parts, somatomedin 0.2 part.
Matched group 3: a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, counts by weight and is made up of following composition: alginate 2 parts, chitosan 3 parts, permanent magnet nanoparticle 1 part, nano silicon 2 parts, somatomedin 0.2 part.
Matched group 4: a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, counts by weight and is made up of following composition: alginate 2 parts, chitosan 3 parts, cross-linking agent 2 parts, permanent magnet nanoparticle 1 part, somatomedin 0.2 part.
Matched group 5: a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, counts by weight and is made up of following composition: alginate 2 parts, chitosan 3 parts, cross-linking agent 2 parts, nano silicon 2 parts, somatomedin 0.2 part.
Matched group 6: a kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof, counts by weight and is made up of following composition: alginate 2 parts, chitosan 3 parts, cross-linking agent 2 parts, permanent magnet nanoparticle 1 part, nano silicon 2 parts.
Carry out antibacterial tests analysis to experimental group and each matched group, result is as follows:
The experimental group that contrast is shown and matched group can find out to have synergism between each composition of magnetic therapy antibacterial anti hemorrhagic dressing prepared by the present invention, indispensable.
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention; For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description, here without the need to also cannot being enumerated all embodiments; All any amendments done within the spirit and principles in the present invention, equivalent to replace and improvement etc., within the protection domain that all should be included in the claims in the present invention.
Claims (8)
1. the dressing of magnetic therapy antibacterial anti hemorrhagic, is characterized in that, counts by weight and is made up of following composition:
Alginate 0.5 ~ 4 part;
Chitosan 0.5 ~ 6 part;
Cross-linking agent 0.5 ~ 4 part;
Permanent magnet nanoparticle 0.1 ~ 2 part;
Nano silicon 0.5 ~ 4 part;
Somatomedin 0.005 ~ 0.5 part.
2. a kind of magnetic therapy antibacterial anti hemorrhagic according to claim 1 dressing, is characterized in that, described alginate is sodium alginate.
3. a kind of magnetic therapy antibacterial anti hemorrhagic according to claim 1 dressing, is characterized in that, the deacetylation of described chitosan is greater than 95%, and molecular mass is 10000 ~ 20000Da.
4. a kind of magnetic therapy antibacterial anti hemorrhagic according to claim 1 dressing, is characterized in that, described cross-linking agent is calcium chloride.
5. a kind of magnetic therapy antibacterial anti hemorrhagic according to claim 1 dressing, is characterized in that, the ferromagnetic nanoparticle of described permanent magnetism is Nd-Fe-B magnetite particle, and magnetic field intensity is 0.15 ~ 0.35 tesla.
6. a kind of magnetic therapy antibacterial anti hemorrhagic according to claim 1 dressing, is characterized in that, the aperture of described nano silicon is 20 ~ 80 μm.
7. a kind of magnetic therapy antibacterial anti hemorrhagic according to claim 1 dressing, is characterized in that, described somatomedin is fibroblast growth factor or epithelical cell growth factor.
8. a preparation method for the magnetic therapy antibacterial anti hemorrhagic dressing as described in claim 1-7 any one, is characterized in that, described preparation method concrete technology step is as follows:
S1. alginate is dissolved in purified water, mixes and stir 0.5h ~ 1h to evenly, being prepared into solution A; Chitosan being dissolved in mass fraction is in the acetic acid solution of 1%, mixes and stirs 0.5h ~ 1h to evenly, being prepared into solution B;
S2. the solution A obtained in S1 slowly added in solution B, mixing also stirring reaction 0.5h ~ 1h, to evenly, is prepared into solution C;
S3. by permanent magnet nanoparticle, the mass fraction be directly distributed to containing nano silicon is carry out the embedding of inorganic silicon source in the sodium hydroxide solution of 8%, the permanent magnet nanoparticle completing the embedding of inorganic silicon source is distributed in aqueous solution, adding cetyl trimethyl ammonium bromide template and ethyl orthosilicate again, to carry out mesoporous silicon oxide coated, after reactant being carried out magnetic separation, washing, the dry removal of impurity, in 500 DEG C of calcinings 5 ~ 7 hours, form magnetic mesoporous silicon dioxide, magnetic mesoporous silicon dioxide is slowly added in solution C obtained in S2, be prepared into solution D;
S4. cross-linking agent is dissolved in purified water, is prepared into solution E, solution D is slowly sprayed in solution E, more spray-dried, being prepared into particle diameter is 100 ~ 200 object flour F;
S5. somatomedin is slowly added in material F, stir, after stirring 0.5h ~ 1h, be mixed into solid powder G;
S6. by solid powder G filling and sealing and outer package, and then carry out radiation sterilization and namely obtain the dressing of described magnetic therapy antibacterial anti hemorrhagic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510699965.9A CN105169460B (en) | 2015-10-26 | 2015-10-26 | A kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510699965.9A CN105169460B (en) | 2015-10-26 | 2015-10-26 | A kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105169460A true CN105169460A (en) | 2015-12-23 |
| CN105169460B CN105169460B (en) | 2018-09-25 |
Family
ID=54892201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510699965.9A Expired - Fee Related CN105169460B (en) | 2015-10-26 | 2015-10-26 | A kind of magnetic therapy antibacterial anti hemorrhagic dressing and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105169460B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105999368A (en) * | 2016-07-28 | 2016-10-12 | 上海建华精细生物制品有限公司 | Epidermal growth factor compound wet wound dressing and preparation method thereof |
| CN106552285A (en) * | 2016-12-08 | 2017-04-05 | 苏州艾博迈尔新材料有限公司 | A kind of magnetic therapy medical function dressing and preparation method thereof |
| CN107865822A (en) * | 2017-08-25 | 2018-04-03 | 中南民族大学 | A kind of preparation method and application for the pharmaceutical hydrogel slow releasing carrier material for mixing mesoporous nano silicon dioxide |
| WO2018064150A1 (en) * | 2016-09-29 | 2018-04-05 | The University Of Memphis Research Foundation | Microbead compositions and methods for delivering an agent |
| WO2018192285A1 (en) * | 2017-04-17 | 2018-10-25 | 王宛婷 | Composite material |
| CN109223314A (en) * | 2018-09-05 | 2019-01-18 | 杨文超 | A kind of Chinese medicine dermatologic patch |
| US10525147B2 (en) | 2018-02-13 | 2020-01-07 | Imam Abdulrahman Bin Faisal University | Bionanocomposite synthesis for would healing |
| CN110755408A (en) * | 2019-09-23 | 2020-02-07 | 中国人民解放军东部战区总医院 | Chitosan antibacterial microspheres capable of slowly releasing growth factors and preparation method and application thereof |
| CN112244966A (en) * | 2020-11-16 | 2021-01-22 | 常州安康医疗器械有限公司 | Clinical drainage puncture ware of internal medicine |
| CN112370565A (en) * | 2020-11-27 | 2021-02-19 | 河南汇博医疗股份有限公司 | Silver-containing long-acting antibacterial dressing |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009130485A2 (en) * | 2008-04-25 | 2009-10-29 | Medtrade Products Limited | Haemostatic material |
| CN101954117A (en) * | 2010-09-27 | 2011-01-26 | 中国人民解放军第三军医大学野战外科研究所 | Hemostatic bacteriostatic biological dressing and preparation method thereof |
| CN102940903A (en) * | 2012-11-20 | 2013-02-27 | 南京理工大学 | Method for preparing medical dressing of polysaccharide cavernous body |
| CN103467771A (en) * | 2013-09-23 | 2013-12-25 | 潍坊美赫曼生物医药科技有限公司 | Preparation method and application of marine organism active hemostasis dressing |
-
2015
- 2015-10-26 CN CN201510699965.9A patent/CN105169460B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009130485A2 (en) * | 2008-04-25 | 2009-10-29 | Medtrade Products Limited | Haemostatic material |
| CN101954117A (en) * | 2010-09-27 | 2011-01-26 | 中国人民解放军第三军医大学野战外科研究所 | Hemostatic bacteriostatic biological dressing and preparation method thereof |
| CN102940903A (en) * | 2012-11-20 | 2013-02-27 | 南京理工大学 | Method for preparing medical dressing of polysaccharide cavernous body |
| CN103467771A (en) * | 2013-09-23 | 2013-12-25 | 潍坊美赫曼生物医药科技有限公司 | Preparation method and application of marine organism active hemostasis dressing |
Non-Patent Citations (2)
| Title |
|---|
| 全国卫生专业技术资格考试专家委员会: "《卫生专业技术资格考试指导》", 29 February 2004 * |
| 梅枭雄等: "医疗条件缺乏环境下的创伤止血药物（材料）研究现状", 《中国药房》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105999368A (en) * | 2016-07-28 | 2016-10-12 | 上海建华精细生物制品有限公司 | Epidermal growth factor compound wet wound dressing and preparation method thereof |
| WO2018064150A1 (en) * | 2016-09-29 | 2018-04-05 | The University Of Memphis Research Foundation | Microbead compositions and methods for delivering an agent |
| CN106552285A (en) * | 2016-12-08 | 2017-04-05 | 苏州艾博迈尔新材料有限公司 | A kind of magnetic therapy medical function dressing and preparation method thereof |
| WO2018192285A1 (en) * | 2017-04-17 | 2018-10-25 | 王宛婷 | Composite material |
| CN107865822A (en) * | 2017-08-25 | 2018-04-03 | 中南民族大学 | A kind of preparation method and application for the pharmaceutical hydrogel slow releasing carrier material for mixing mesoporous nano silicon dioxide |
| US10525147B2 (en) | 2018-02-13 | 2020-01-07 | Imam Abdulrahman Bin Faisal University | Bionanocomposite synthesis for would healing |
| CN109223314A (en) * | 2018-09-05 | 2019-01-18 | 杨文超 | A kind of Chinese medicine dermatologic patch |
| CN110755408A (en) * | 2019-09-23 | 2020-02-07 | 中国人民解放军东部战区总医院 | Chitosan antibacterial microspheres capable of slowly releasing growth factors and preparation method and application thereof |
| CN110755408B (en) * | 2019-09-23 | 2021-09-03 | 中国人民解放军东部战区总医院 | Chitosan antibacterial microspheres capable of slowly releasing growth factors and preparation method and application thereof |
| CN112244966A (en) * | 2020-11-16 | 2021-01-22 | 常州安康医疗器械有限公司 | Clinical drainage puncture ware of internal medicine |
| CN112370565A (en) * | 2020-11-27 | 2021-02-19 | 河南汇博医疗股份有限公司 | Silver-containing long-acting antibacterial dressing |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105169460B (en) | 2018-09-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105169460A (en) | Magnetically therapeutic antibacterial haemostatic wound dressing and preparation method thereof | |
| CN102600494B (en) | Polysaccharide combination and preparation method and application thereof | |
| AU2019204438B2 (en) | Homeopathic therapeutic method and compositions | |
| CN106377791A (en) | Bamboo alginate functional dressing and preparation method thereof | |
| CN111097067A (en) | Antibacterial medical dressing for promoting wound to heal rapidly and preparation method thereof | |
| CN107469139A (en) | A kind of preparation method of high intensity wound dressing with antibacterial and anti-inflammatory effects | |
| Jiang et al. | Carboxymethyl chitosan-based multifunctional hydrogels incorporated with photothermal therapy against drug-resistant bacterial wound infection | |
| RU2699362C2 (en) | Composition based on cerium dioxide nanoparticles and brown algae polysaccharides for treating wounds | |
| Zhou et al. | Metal-phenolic self-assembly shielded probiotics in hydrogel reinforced wound healing with antibiotic treatment | |
| CN1799639A (en) | Inflammation-eliminating, pain-stopping, bacteria-resisting, pain-relieving soluble hemostatic gauze and its processing method | |
| WO2020180202A1 (en) | Composition based on cerium dioxide nanoparticles and brown algae polysaccharides for wound treatment | |
| CN103301151A (en) | Silver-iodide-doped bioactive glass as well as preparation method and application of silver-iodide-doped bioactive glass | |
| CN109395154A (en) | A kind of high porosity carries the preparation method of medicine wound dressing | |
| CN103656736A (en) | Antibacterial finishing agent for medical dressing | |
| CN109432483B (en) | Medical dressing for accelerating wound healing and preparation method and application thereof | |
| Fang et al. | Highly water-absorptive and antibacterial hydrogel dressings for rapid postoperative detumescence | |
| CN103463616B (en) | A kind of chitosan and tripeptides composition and method of making the same and application | |
| CN113425889A (en) | Antibacterial hemostatic sponge and preparation method and application thereof | |
| CN101317963B (en) | Traditional Chinese medicine for treating exogenous injury and ulcer and preparation method thereof | |
| CN203017195U (en) | Band-aid with silver ion anti-bacterial function | |
| Saifzadeh et al. | Effect of a static magnetic field on bone healing in the dog: radiographic and histopathological studies | |
| CN110251716A (en) | A kind of wound care gel dressing and preparation method thereof | |
| CN112843328B (en) | Preparation method of abalone shell powder/ZnO composite material-doped intelligent hydrogel wound dressing with antibacterial effect | |
| CN111821504B (en) | Silver ion disinfection gel and preparation method thereof | |
| Lu et al. | Multifunctional carbon quantum dots decorated self-healing hydrogel for highly effective treatment of superbug infected wounds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180925 |