CN105152784B - A kind of anti-copper stress corn seed treatment and the preparation method and application thereof - Google Patents
A kind of anti-copper stress corn seed treatment and the preparation method and application thereof Download PDFInfo
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Abstract
A kind of anti-copper stress corn seed treatment and the preparation method and application thereof is related to a kind of corn seed treatment and the preparation method and application thereof.A kind of anti-copper stress corn seed treatment of present invention offer and the preparation method and application thereof.The seed dressing includes plant growth regulator, trace element, chelating agent, adhesive, wetting agent, colorant and water.Method：Plant growth regulator, trace element, chelating agent, adhesive, surfactant and water are successively added in mixer, are uniformly mixed, anti-copper is made and coerces corn seed treatment.The seed dressing can improve corn seed germination rate and germinating energy, improve root growth and development and Leaf Physiology biochemical character, and seedlings are uniform and strong, have controllability and persistence in anti-copper stress and nutrient supply.The present invention is used for corn planting field.
Description
Technical field
The present invention relates to a kind of corn seed treatments and the preparation method and application thereof.
Background technology
In recent years, it is influenced by Crops Planting Structure Adjustment, Chinese maize industry development is swift and violent, this just needs more to pacify
Complete effective soil carrys out maize planting, just can ensure that the high yield and stable yields of corn.Corn seeding area and yield are all in increase year by year
The trend added, corn total yield account for 50% or more total grain output.By taking Heilongjiang Province as an example, cereal crops sown area reaches within 2015
2140000000 mu, wherein corn seeding area reaches 120,000,000 mu, and has the tendency that further increasing cultivated area in future.
Plant is an open system, while constantly absorbing substance, energy and information from external environment,
It is influenced by various ecological factors.The wherein basic factor the most key in soil elements affect corn growth.Relatively
In other environmental pollution states such as atmosphere pollution, water pollution, heavy metal pollution of soil then has the characteristics that concealment, hysteresis quality,
Significant damage is often had resulted in when being discovered by supervision department and society.Studies have shown that China be more than 16% arable land by heavy metal
Pollution (research [J] the China agronomy that Li Geng flies 3 heavy metal species accumulation ability of Different Crop pair near the mining areas is notified to, and 2012,
28(26)：257-261).The publication of Ministry of Land and Resources 2013 statistics indicate that, China is by the cultivated area of moderate and serious pollution
About 50,000,000 mu, it is equivalent to the Belgian national territorial area of European countries, China is every year because of the grain of heavy metal pollution of soil
About 12,000,000 tons, direct economic loss is at 20,000,000,000 yuan or so.Caused by being polluted due to heavy metal in soil with China in Recent Years
Great environmental event continuously emerges, and country also in gradual formulation and improves about the law of heavy metal pollution of soil, regulation and phase
Pass policy increases the supporting dynamics administered to heavy metal pollution of soil, has gradually carried out heavy metal pollution of soil and has administered pilot work
Make.
The accumulation of heavy metal in soil is derived mainly from following reason.
Firstly, since the random discharge of three industrial wastes " waste residue, waste liquid and exhaust gas " and the excessive application of pesticide, chemical fertilizer,
So that the Accumulation of heavy metals in soil is all higher than ever.With 11,780,000 hectares of the black soil of Northeast China in Grain Main-production Region
For, in front of the mountains fluctuating tableland of the area distribution in the Northeast Xiaoxinanlin Mountains and Changbai Mountain west side.In recent years, black earth arable land face
Product and productivity are just on a declining curve, and the content of organic matter of black earth declines, and deep variation has occurred in physicochemical property.Study table
It is bright, select 191 pedotheques in haplic phaeozem 3 basic sample area, different heavy metal elements domain of the existence difference on the whole, table
Cu, Zn, Cr in layer, Hg are that Helen is higher than other two places, and Pb is the Gongzhuling Bei'ans ＞ Helen ＞；Subsurface stratum content slightly drops
It is low, it is point different to be equal to surface layer (pearl, local point of Yan Li black earth heavy metal elements are correlated in Wang Tieyu, Wang Jingkuan, Zhou Min, side development
Different and environmental risk [J] agro-environment science journals, 2004,23 (2)：272-276).Heavy metal in soil is polluted and its is administered
It works very urgent.
Secondly, with the improvement of people's living standards, animal husbandry is grown rapidly as main pillar industry.With black
For the province of Longjiang, 2014, animal husbandry value added realized 557.1 hundred million yuan, increases by 4.4% on a year-on-year basis.228.19 ten thousand tons of meat total yield,
Increase by 4.19% on a year-on-year basis.556.58 ten thousand tons of milk yield, increases by 7.40% on a year-on-year basis.Heilongjiang Province's meat in 2014, milk yield speedup
Respectively higher than Chinese 3.4 percentage points and 0.8 percentage point of average level.Since Braude (1945) has found that diet adds for the first time
The copper of high dose can accelerate the growth of store pig, and since improving efficiency of feed utilization, and copper, zinc of high dose etc. have anti-
The somatotrophic special efficacy of bacterium is generally received by animal nutritionist and the pig producer, and in the daily ration of grower pigs, birds etc.
Middle extensive use.Since copper all plays emphatically metalloenzyme, iron metabolism, hemoglobin synthesis, red blood cell and bon e formation in animal body
Huge (Miller, Ullrey D E, Elliis D J.Comparison of the copper sulfate of role and influence wanted
And selected antibiotic for gorwing-finishing swine [J] .J Anim Sci, 1969,29：
140).High-caliber copper (being more than 200mg/ ㎏) is added in Animal husbandry production as a kind of efficient and cheap growth promotion addition
Agent largely uses in livestock and poultry breeding industry.Thus the high-copper excreta brought is rich in farm's surrounding soil and water body
The phenomenon that collecting, leading to crop production reduction and quality decline is increasingly serious.But research shows that：The copper of excessive addition not only to weightening and
Feed consumption has no improvement, and also results in a large amount of copper and cause environmental pollution (Fu Yuehua, Xiong little Wen, Ouyang in vitro with excrement exclusion
Gram a species of orchid ecoalimentals and environmental protection [J] Agricultural University Of Jiangxi journal, 1997,19 (4)：93-96).On the other hand, addition
Copper by the enrichment of animal body make its toxicity enhance, into people's food chain after can be accumulated in human body, generate it is many it is bad after,
Even it is detrimental to health.
Copper be plant growth must one of required trace element, but heavy metal copper in soil content increases to jade
The growth of rice is seriously unfavorable, is in particular in following three points.
First, the accumulation of excessive copper influences the photosynthesis of maize leaf in soil.Excessive Cu influences the light of blade
Cooperation is used, and the structure of chlorophyll content content, Pigment-protein Complexes can be destroyed, the light of primary photochemical reaction and PS II
Close electron-transport be obstructed (E., Aro, E.M., andE.Increase in the quantum
yield of photo inhibition contributes to copper toxicity in vivo[J].Plant
Physiol, 1998,117,619-627).Cu in plant cell can promote to plant by Haber-Weiss and Fenton reactions
Object cellular accumulation hydroxy radical (.OH) and hydrogen peroxide (H2O2).The active oxygen of accumulation can cause the peroxide of lipid and protein
Change acts on, thus the normal physiological metabolism of severe jamming plant (Mysliwa-Kurdziel, B., and Strzalka,
K.Influence of metals on biosynthesis of photosynthetic pigments[M].In
Physiology and Biochemistry of Metal Toxicity and Tolerance in Plants,
M.N.V.Prasad and K.Strzatka eds(The Netherlands：Kluwer Academic Publishers),
2002, pp.201-227.).
Second is that the accumulation of excessive copper influences the change of enzymatic activity in Antioxidant Enzyme Systems in Leaves of Maize Seedlings in soil
Change.With the increase of Cu mass concentrations (5-160mg/L), superoxide dismutase (SOD), peroxidase in seedling leaves
(POD) activity increases, and catalase (CAT) activity first increases to be declined afterwards.Copper stress also results in the damage of the oxidation in plant
Wound, mda content improve with Cu processing mass concentrations and are increased.The Cu of corn seedling accumulation is in vivo with different chemical shapes
State be distributed, the mobility that internal Cu is reduced by Binding in vivo mechanism be corn seedling resist Cu contamination hazards important mechanisms it
One (bear think of, Lin Aijun, Song Liang, Wei Lianshuan, the Beijing toxicity and its Mechanism Study [J] of Li Qiang Cu-W ore deposits to corn seedling
Work college journal (natural science edition), 2013,40 (6)：82-87).
Third, the accumulation of excessive copper influences the growth of maize seedling roots and overground part in soil.5-20 μm of ol/L's
Cu handles apparent inhibition maize seedling roots growth in 10 days, and reduces the dry matter of corn seedling, and with increasing corn seedling
Top and root system amount containing Cu；The Cu that corn seedling absorbs largely is accumulated in root system, and part cloth is less on the ground.Cu processing is also dropped
Chlorophyll content and Fv/Fm, ETR, qP and qy value (Hu Zhubing, Chen Yahua, Wang Guiping, the Shen Zhen states copper stress of low maize leaf
Influence [J] BULLETIN OF BOTANY Vol.s to growth of maize, chlorophyll fluorescence parameters and activities of antioxidant enzymes, 2006,23 (2)：
129-137)。
Currently, the method that heavy metal-polluted soil Cu-W ore deposit is administered mainly has two major classes：First, by changing heavy metal in soil
The existing forms of pollutant.It is fixed in the soil, makes heavy metal far from food chain, to reduce the harm to human body.Two
It is to recycle heavy metal using special plant to the absorbability of heavy metal ion or using engineering method, directly reduces weight in soil
The content of metal.Generally comprise engineering repairing method, agriculture repairing method and biological restoration.Wherein at home and abroad in numerous items,
Fast due to repairing speed, curing/stabilizing technology (engineering repairing method) is widely used.Environmental Protection Department is put into effect within 2009
《Contaminated site soil restoring technology directive/guide (exposure draft)》, the improvement to time and each restorative procedure needed for restorative procedure
Cost gives guidance.Although the cost of phytoremediation single is relatively low, need to repeat for many years, totle drilling cost unlike
Other repairing methods are relatively low.
Excess copper can enter entire food chain by the absorption of plant body.The corn applied in production at present is mixed
Pest and disease damage of the agent primarily directed to soil-borne class is planted, for example prevents subterranean pest-insect, rough dwarf disease, smut, northern and southern leaf blight etc..
About alleviation heavy metal stress (copper is coerced, Cd stress, arsenic stress etc.), the corn seed dressing of Saline Alkali Stress etc. in maize production
Agent type is less, and the regular product for obtaining national sector's approval also extremely lacks, and market is huge.Therefore, a kind of corn is prepared to mix
Kind agent mitigates threat of the Soil Copper stress to corn growth, enhances corn anti-adversity ability, is corn seed germination and growth creation
One good ecological environment provides an effective way for Heilungkiang Cu-W ore deposit area corn planting, ensures the Northeast,
The maize production in especially heavy metal pollution area is of great significance.
It is found by retrieving, patent application CN104162537A《A kind of reasonable method using copper polluted soil》Patent
Technology discloses and plants Yellowflower evening primrose in copper polluted soil, and copper polluted soil, reduction pollution are rationally utilized to realize
Soil is laid idle discarded purpose.The technology belongs to phytoremediation technology, is to repair Cu-W ore deposit plot using Yellowflower evening primrose, so
And requirement of the Yellowflower evening primrose for growing environment and weather conditions itself is harsh, and planting cost is higher, along with chrysanthemum month
See that the management means such as the careless prevention and control of plant diseases, pest control of itself are complicated, therefore actually cost is higher, is unfavorable for promoting.Patent application
201310690855.7《A kind of chemical leaching repairing method for copper polluted soil》, chemical leaching be easy to cause underground water
Soil acidification is polluted and causes, and cost is higher, it is complex using technology, it is not easy to grasp the technology that uses, and work in production
Work amount is larger, and when various elution agent concentrations grasp bad, can have certain injury to planting plant in the soil
Effect.
Invention content
The purpose of the present invention is to provide a kind of anti-copper to coerce corn seed treatment and the preparation method and application thereof.For northeast
In animal husbandry development along with farmland Cu-W ore deposit endanger serious problem, providing a kind of can improve corn seed germination rate and hair
Bud gesture improves root growth and development and Leaf Physiology biochemical character, and seedlings are uniform and strong, and having in anti-copper stress and nutrient supply can
Control property and persistent corn seed treatment.
The present invention anti-copper stress corn seed treatment includes plant growth regulator, trace element, chelating agent, adhesive, wet
Moisten agent, colorant and water, wherein plant growth regulator is by 2- (3,4- dichlorophenoxy) triethylamine, γ-aminobutyric acid, benzene peptide
Amino acid and Prohexadione calcium composition, the trace element are made of potassium citrate, zinc chloride, calcium nitrate and manganese sulfate, the chelating
Agent is ethylenediamine tetra-acetic acid, and described adhesive is Sodium Polyacrylate, and the wetting agent is neopelex, the coloring
Agent is that dyestuff is red.
The anti-copper of the present invention coerces corn seed treatment by mass percentage by the 2- of 0.02%-2% (3,4- dichlorophenoxy)
Triethylamine, the γ-aminobutyric acid of 0.01%-2%, the benzene peptide amino acid of 0.01%-1%, 0.01%-2% Prohexadione calcium,
The sulfuric acid of the potassium citrate of 0.05%-3%, the zinc chloride of 0.05%-3%, the calcium nitrate of 0.05%-3%, 0.05%-3%
The dodecyl benzene sulfonic acid of manganese, the ethylenediamine tetra-acetic acid of 0.5%-3%, the Sodium Polyacrylate of 0.2%-1%, 0.02%-0.5%
Sodium, 0.05%-0.5% dyestuff is red and the water of surplus composition.
Plant growth regulator 2- (3, the 4- dichlorophenoxy) triethylamine, adopted name DCPTA, trade name volume increase
Amine.It is it is a kind of to growth and development of plants there are many excellent properties active material.Its nonhazardous, may be used also at pollution-free, noresidue
Greatly improve chemical fertilizer utilization ratio.Using DCPTA crop also in disease and insect resistance, resist barren, drought resisting, freeze proof etc. show to dash forward
The effect gone out.DCPTA can be compounded with multiple element, and foliar fertilizer, punching fertilising, base manure is made；Can also and fungicide, insecticide,
Mixture of herbicides uses, and enhances the disease resistance of plant, improves drug effect.
The plant growth regulator γ-aminobutyric acid, adopted name GABA are distributed widely in animal and plant body.GABA
It is a kind of important inhibitory neurotransmitter, it participates in a variety of metabolic activities of animals and plants, and physiological activity is strong, is producing in recent years
On be used widely.
The plant growth regulator benzene peptide amino acid, English name Phenyl-phthalamicacid have and promote nutrients
Matter is transported to growing point, promotes Chlorophyll synthesis, induces the effect of bud is at flower result, can be with other insecticides, sterilization
The seed dressings such as agent class are applied.
The plant growth regulator Prohexadione calcium, English name Prohexadione calcium, Prohexadione calcium can take
For triazole type delayer, environmentally friendly safety inhibits the synthesis of gibberellic acid, comes after capable of being absorbed by plant root and blade
Have the function that shorten cane elongation, there is hestening rooting and lateral bud to grow, keep leaf dark green, promote Development of ear and grain, improve
The resistance of plant, has broad application prospects in production.
The chelating agent is ethylenediamine tetra-acetic acid (EDTA), English name Ethylene Diamine Tetraacetic
Acid, EDTA are a kind of good compounding agents in chemistry, and there are six coordination atom, the complexs of formation to be called chelate for it,
EDTA is often used in complexometric titration, the content of metal ion is usually measured, in biologic applications, for excluding major part
The interference of transition metals ion (such as iron (III), nickel (II), manganese (II)).It can be in not shadow in protein engineering and experiment
Interfering ion is removed in the case of ringing protein function.EDTA has many uses, and can be used as the drift of color sensitive material washing processing
White fixing solution, dyeing assistant, fiber treatment auxiliary agent, cosmetic additive agent, blood anticoagulant, detergent, stabilizer, synthetic rubber
Polymerization initiator.In addition to sodium salt, also various salt such as ammonium salt and iron, magnesium, calcium, copper, manganese, zinc, cobalt, aluminium.Main application：Ethylenediamine
Tetraacethyl is mainly used as complexing agent, is widely used in water treatment agent, washing additive, illumination chemicals, papermaking chemical product, oil field
Chemicals, boilercompound and analytical reagent.
Described adhesive is Sodium Polyacrylate, and Sodium polyacrylate, solid product is white (or light yellow) block
Shape or powder, liquid form product are colourless (or faint yellow) thick liquid, are a kind of new functional macromolecule materials, multi-purpose to make surface
Activating agent.Contain a large amount of strongly hydrophilic group in the strand of Sodium Polyacrylate, the media such as cold water, propylene glycol can be dissolved in,
Temperature change is stablized, has the function of fixing metal ions.Dry products can be conducted oneself with dignity in air with moisture absorption 10%, and it is high
Water-absorbing resin can then absorb the distilled water of 1000 times of dead weight or more.Molecular weight is used as flocculant, and eating 10 or more
Make tackifier, emulsifying dispersant etc. in product industry.
The wetting agent is neopelex, also referred to as tetrapropylene benzene sodium sulfonate, white or yellowish toner
Shape or chip solid.It is dissolved in water and forms translucent solution.It is mainly used as anionic surfactant, production cost is low, property
Can be good, it can also be used to manufacture organic amine salt.
The preparation method of above-mentioned anti-copper stress corn seed treatment, carries out according to the following steps：
One, 2- (3,4- dichlorophenoxy) triethylamine of 0.02%-2%, 0.01%-2% are weighed by mass percentage
γ-aminobutyric acid, the benzene peptide amino acid of 0.01%-1%, the Prohexadione calcium of 0.01%-2%, 0.05%-3% potassium citrate,
The ethylenediamine tetraacetic of the zinc chloride of 0.05%-3%, the calcium nitrate of 0.05%-3%, the manganese sulfate of 0.05%-3%, 0.5%-3%
The dyestuff of acetic acid, the Sodium Polyacrylate of 0.2%-1%, the neopelex of 0.02%-0.5%, 0.05%-0.5%
Red and surplus water；Wherein 2- (3,4- dichlorophenoxy) triethylamine, γ-aminobutyric acid, benzene peptide amino acid and Prohexadione calcium are to plant
Object growth regulator forms, and potassium citrate, zinc chloride, calcium nitrate and manganese sulfate are trace element, and ethylenediamine tetra-acetic acid is chelating
Agent, Sodium Polyacrylate are adhesive, and neopelex is wetting agent, and red dyestuff is colorant；
Two, plant growth regulator, trace element, chelating agent, adhesive, surfactant and the water weighed step 1
It is successively added in mixer, is uniformly mixed, anti-copper is made and coerces corn seed treatment.
The concrete application method of above-mentioned anti-copper stress corn seed treatment is：
The mass ratio of corn seed treatment and seed is 1：50.
0.1kg cold water (25 DEG C) is first added into container, 20g corn seed treatments are equably spread in water at a slow speed, while spreading
Side is stirred, and after stirring 15 minutes, 1kg corn seeds is poured into container, are stirred evenly so that every the surface of the seed is in uniform
Color, then spreading naturally dry out can sow after 4-5 hours.Placement a period of time is broadcast again after seed dressing can also be coated in advance
Kind.The operation of Mechanization sowing is practical in being planted simultaneously for the ease of northeast corn, and suitable talcum powder can be added prior to seeding
The seed mixing dressed seed, sows conducive to machine and improves seeding quality.
The principle of the present invention is：
Plant growth regulator (2- (3,4- dichlorophenoxy) triethylamine, γ-aminobutyric acid, benzene peptide amino acid, Prohexadione calcium
Deng) corn seed sprouting and relevant physiological metabolic process under adverse circumstance can be improved, the balance of active oxygen metabolism is had adjusted, is stablized
Membrane structure, improves the water absorbing capacity of root system, directly takes part in corn to the reparation of copper stress damage and the resistance of adverse circumstance
Journey.The potassium ion of suitable concentration simultaneously, zinc ion, calcium ion and manganese ion synergistic effect effectively enhance the lower jade of copper stress
Nutrition needed for root system and leaf photosynthesis growth after rice seed sprouting.Plant growth regulator and trace element it is common
Under effect, K is improved on plasma membrane level+- ATPase, Zn2+- ATPase, Ca2+- ATPase, Mn2+The enzymatic activitys such as-ATPase,
It avoids the tender separate living tissue of corn aggrieved, promotes root system and blade cell division, promote plant of the corn under copper stress conditions
Object converts the utilization of a great number of elements (N P and K) and substance.
Beneficial effects of the present invention：
The present invention, for active ingredient, promotes life of the corn in copper coerces soil with plant growth regulator and trace element
Long developmental potency, percentage of seedgermination improve, the strengthening root thick second.Threat of the Soil Copper stress to corn growth, enzymatic activity can be mitigated
And metabolic function enhancing, corn anti-adversity ability is enhanced, is that a good ecological environment is created in corn seed germination and growth,
An effective way is provided for Heilungkiang Cu-W ore deposit area corn planting, is had very in strengthening copper polluted soil phytoremediation
Good application potential.
(1) present invention is through Seed germination, potted plant experiment and field experiment, in germination percentage, germination index, Root Characteristics parameter
Pole antioxidant enzyme activities in leaves, the indices such as dry-matter accumulation are above control group, experiments have shown that the present invention coerces item to copper
Course of Corn Seed Germination and growth of seedling have good facilitation under part.
(2) field experiment shows the corn after seed dressing in copper coerces soil, and having preferably prevents maize head dust-brand
The effect of disease.
(3) easy to use, convenient for sowing operation.Dosage is few, at low cost, time saving and energy saving, increasing both production and income remarkable benefit.
Cost per mu only needs 5 yuan, and input-output ratio has certain popularization 30% or more in modernization crop chemistry control engineering
Value.
(4) raw material components of the present invention are to people and animals' nonhazardous, low-carbon environment-friendly.
Specific implementation mode
Technical solution of the present invention is not limited to act specific implementation mode set forth below, further includes between each specific implementation mode
Arbitrary combination.
Specific implementation mode one：The anti-copper stress corn seed treatment of present embodiment includes plant growth regulator, micro member
Element, chelating agent, adhesive, wetting agent, colorant and water, wherein plant growth regulator are by 2- (3,4- dichlorophenoxy) three second
Amine, γ-aminobutyric acid, benzene peptide amino acid and Prohexadione calcium composition, it is described trace element by potassium citrate, zinc chloride, calcium nitrate and
Manganese sulfate forms, and the chelating agent is ethylenediamine tetra-acetic acid, and described adhesive is Sodium Polyacrylate, and the wetting agent is dodecane
Base benzene sulfonic acid sodium salt, the colorant are that dyestuff is red.
The ability of corn copper stress, corn germination gesture, germination percentage, germination can be effectively relieved in the seed dressing of present embodiment
Index, vitality index and coleoptile length increase；Improve root characteristics parameter, root activity increases；Photosynthetic characteristics and
Antioxidant Enzyme Systems significantly improve, and dry-matter accumulation increases；Maize head smut incidence significantly reduces, and above is corn adverse circumstance
Under the conditions of yield composition establish good basis.To solve the agriculture chemical soil that excessively application and animal husbandry development are brought
Cu-W ore deposit administers difficult problem, and the Course of Corn Seed Germination for solving Soil Copper stress plot is poor, and emergence is slow, and growth and development is suppressed,
The problems such as low output.The corn seed treatment being prepared can effectively improve corn resistance, enough mitigate Soil Copper stress to corn
The threat of growth, enhance corn anti-adversity ability, be corn seed germination and growth create a good ecological environment, have compared with
Good effect of increasing production.Preparation method of the present invention is simple, easy to use, environmentally friendly and cost is relatively low, use easy to spread.
Specific implementation mode two：The present embodiment is different from the first embodiment in that：Anti- copper coerces corn seed treatment
By mass percentage by the 2- of 0.02%-2% (3,4- dichlorophenoxy) triethylamine, 0.01%-2% γ-aminobutyric acid,
The benzene peptide amino acid of 0.01%-1%, the chlorine of the Prohexadione calcium of 0.01%-2%, the potassium citrate of 0.05%-3%, 0.05%-3%
Change zinc, the calcium nitrate of 0.05%-3%, the manganese sulfate of 0.05%-3%, the ethylenediamine tetra-acetic acid of 0.5%-3%, 0.2%-1%
Sodium Polyacrylate, the neopelex of 0.02%-0.5%, 0.05%-0.5% dyestuff is red and the water group of surplus
At.It is other same as the specific embodiment one.
Specific implementation mode three：The present embodiment is different from the first embodiment in that：Anti- copper coerces corn seed treatment
By mass percentage by 1% 2- (3,4- dichlorophenoxy) triethylamine, 1% γ-aminobutyric acid, 1% benzene peptide amino acid,
1% Prohexadione calcium, 1% potassium citrate, 1% zinc chloride, 1% calcium nitrate, 1% manganese sulfate, 1% ethylenediamine tetraacetic
Acetic acid, 0.4% Sodium Polyacrylate, 1% neopelex, 1% dyestuff is red and the water of surplus composition.It is other with
Specific implementation mode one is identical.
Specific implementation mode four：The present embodiment is different from the first embodiment in that：Anti- copper coerces corn seed treatment
Preparation method, carry out according to the following steps：
One, 2- (3,4- dichlorophenoxy) triethylamine of 0.02%-2%, 0.01%-2% are weighed by mass percentage
γ-aminobutyric acid, the benzene peptide amino acid of 0.01%-1%, the Prohexadione calcium of 0.01%-2%, 0.05%-3% potassium citrate,
The ethylenediamine tetraacetic of the zinc chloride of 0.05%-3%, the calcium nitrate of 0.05%-3%, the manganese sulfate of 0.05%-3%, 0.5%-3%
The dyestuff of acetic acid, the Sodium Polyacrylate of 0.2%-1%, the neopelex of 0.02%-0.5%, 0.05%-0.5%
Red and surplus water；Wherein 2- (3,4- dichlorophenoxy) triethylamine, γ-aminobutyric acid, benzene peptide amino acid and Prohexadione calcium are to plant
Object growth regulator forms, and potassium citrate, zinc chloride, calcium nitrate and manganese sulfate are trace element, and ethylenediamine tetra-acetic acid is chelating
Agent, Sodium Polyacrylate are adhesive, and neopelex is wetting agent, and red dyestuff is colorant；
Two, plant growth regulator, trace element, chelating agent, adhesive, surfactant and the water weighed step 1
It is successively added in mixer, is uniformly mixed, anti-copper is made and coerces corn seed treatment.It is other same as the specific embodiment one.
Specific implementation mode five：The present embodiment is different from the first embodiment in that：Anti- copper coerces corn seed treatment
Application process be：
Cold water is first added into container, corn seed treatment is equably spread at a slow speed in water, Bian Sabian stirrings stir 15 points
Zhong Hou pours into corn seed in container, stirs evenly so that every the surface of the seed is in uniform color, is then spread out certainly
It can be sowed after so drying 4-5 hours；Wherein the mass ratio of corn seed treatment and seed is 1：50, the matter of corn seed treatment and water
Amount is than being 1：50, the temperature of the cold water is 15~35 DEG C.It is other same as the specific embodiment one.
Specific implementation mode six：Present embodiment is unlike specific implementation mode five：Prior to seeding by talcum powder and
The corn seed mixing dressed seed, then sowed.It is other identical as specific implementation mode five.
Specific implementation mode seven：Present embodiment is unlike specific implementation mode five：The temperature of the cold water is 25
℃.It is other identical as specific implementation mode five.
Following experiment is carried out for verification beneficial effects of the present invention：
Substance used in following embodiment is conventional chemical reagent.
Embodiment 1：The preparation of corn seed treatment 1
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 0.2%, γ-aminobutyric acid 0.1%, benzene peptide amino acid 0.1%, Prohexadione calcium
0.1%, potassium citrate 1%, zinc chloride 1%, calcium nitrate 1%, manganese sulfate 1%, ethylenediamine tetra-acetic acid 1%, Sodium Polyacrylate
0.4%, neopelex 0.1%, dyestuff red 0.1%, remaining is water.
Mixer is successively added in plant growth regulator, trace element, chelating agent, adhesive, surfactant and water
In, it is uniformly mixed, anti-copper is made and coerces corn seed treatment.
Embodiment 2：The preparation of corn seed treatment 2
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 0.5%, γ-aminobutyric acid 0.05%, benzene peptide amino acid 0.3% adjust naphthenic acid
Calcium 0.3%, potassium citrate 0.5%, zinc chloride 0.6%, calcium nitrate 0.7%, manganese sulfate 0.7%, ethylenediamine tetra-acetic acid 1.5%,
Sodium Polyacrylate 0.3%, neopelex 0.2%, dyestuff red 0.1%, remaining is water.Other and specific implementation mode
One of it is identical.
Embodiment 3：The preparation of corn seed treatment 3
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 0.4%, γ-aminobutyric acid 0.4%, benzene peptide amino acid 0.4%, Prohexadione calcium
0.5%, potassium citrate 1.2%, zinc chloride 1.5%, calcium nitrate 1.3%, manganese sulfate 1.4%, ethylenediamine tetra-acetic acid 1.2%, gather
Sodium acrylate 0.5%, neopelex 0.04%, dyestuff red 0.06%, remaining is water.Other and specific implementation mode
One of it is identical.
Embodiment 4：The preparation of corn seed treatment 4
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 0.8%, γ-aminobutyric acid 0.75%, benzene peptide amino acid 0.84% adjust naphthenic acid
Calcium 0.95%, potassium citrate 0.85%, zinc chloride 0.5%, calcium nitrate 0.75%, manganese sulfate 0.85%, ethylenediamine tetra-acetic acid
0.9%, Sodium Polyacrylate 0.6%, neopelex 0.5%, dyestuff red 0.02%, remaining is water.Other with it is specific
One of embodiment is identical.
Embodiment 5：The preparation of corn seed treatment 5
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 1%, γ-aminobutyric acid 1%, benzene peptide amino acid 0.9%, Prohexadione calcium
0.9%, potassium citrate 1.2%, zinc chloride 1.1%, calcium nitrate 1.5%, manganese sulfate 1.2%, ethylenediamine tetra-acetic acid 1.1%, gather
Sodium acrylate 0.9%, neopelex 0.4%, dyestuff red 0.2%, remaining is water.Other and specific implementation mode it
One is identical.
Embodiment 6：The preparation of corn seed treatment 6
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 1.3%, γ-aminobutyric acid 1.5%, benzene peptide amino acid 1.2%, Prohexadione calcium
0.9%, potassium citrate 1.3%, zinc chloride 1.1%, calcium nitrate 1.1%, manganese sulfate 1.2%, ethylenediamine tetra-acetic acid 1.5%, gather
Sodium acrylate 0.4%, neopelex 0.5%, dyestuff red 0.04%, remaining is water.Other and specific implementation mode
One of it is identical.
Embodiment 7：The preparation of corn seed treatment 7
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 1.6%, γ-aminobutyric acid 1.3%, benzene peptide amino acid 1.4%, Prohexadione calcium
1%, potassium citrate 1.1%, zinc chloride 0.9%, calcium nitrate 1%, manganese sulfate 1%, ethylenediamine tetra-acetic acid 1.2%, polyacrylic acid
Sodium 0.4%, neopelex 0.25%, dyestuff red 0.25%, remaining is water.One of other and specific implementation mode phase
Together.
Embodiment 8：The preparation of corn seed treatment 8
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 1.2%, γ-aminobutyric acid 1.15%, benzene peptide amino acid 1%, Prohexadione calcium
1%, potassium citrate 0.9%, zinc chloride 1.2%, calcium nitrate 1.2%, manganese sulfate 1.2%, ethylenediamine tetra-acetic acid 1.2%, poly- third
Olefin(e) acid sodium 0.5%, neopelex 0.1%, dyestuff red 0.1%, remaining is water.One of other and specific implementation mode
It is identical.
Embodiment 9：The preparation of corn seed treatment 9
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 1.6%, γ-aminobutyric acid 1.9%, benzene peptide amino acid 2%, Prohexadione calcium
1.8%, potassium citrate 1.6%, zinc chloride 1.8%, calcium nitrate 1.2%, manganese sulfate 1.4%, ethylenediamine tetra-acetic acid 1%, poly- third
Olefin(e) acid sodium 0.6%, neopelex 0.5%, dyestuff red 0.4%, remaining is water.One of other and specific implementation mode
It is identical.
Embodiment 10：The preparation of corn seed treatment 10
The present embodiment corn seed treatment is made of following masses percentage composition raw material：
2- (3,4- dichlorophenoxy) triethylamine 1.9%, γ-aminobutyric acid 1.3%, benzene peptide amino acid 1.4%, Prohexadione calcium
0.9%, potassium citrate 1.2%, zinc chloride 1.4%, calcium nitrate 1.2%, manganese sulfate 1.2%, ethylenediamine tetra-acetic acid 1%, poly- third
Olefin(e) acid sodium 0.4%, neopelex 0.4%, dyestuff red 0.35%, remaining is water.Other and specific implementation mode it
One is identical.
Corn seed treatment prepared by above-described embodiment carries out following experiment, real through Seed germination, potted plant experiment and crop field
It tests, in germination percentage, germination index, seedling fresh weight, root item number, Root Parameters, relative physiologic index, the items such as dry-matter accumulation refer to
Mark is above control group, experiments have shown that corn seed treatment of the present invention has Course of Corn Seed Germination under copper stress conditions and growth of seedling
There is good facilitation.
Experiment 1：Influence of the corn seed treatment to Course of Corn Seed Germination under copper stressful environmental condition.
Choose two kinds of maize genotypes seeds (first 335 Hes of jade planted extensively in the uniform Heilungkiang of full seed
Zheng Dan 958), take corn seed treatment 1~10 made above to carry out Course of Corn Seed Germination experiment, while the jade that setting is not dressed seed
Rice seed is control.
The application method of corn seed treatment is：It takes 20g seed dressings to be watered 0.1kg, 0.1kg cold water is first added into container,
Seed dressing is equably spread at a slow speed in water, Bian Sabian stirrings pour into 1kg corn seeds in container, instead after stirring 15 minutes
Multiple stirring, until uniformly coating so that every the surface of the seed is in uniform color, spreading naturally dry out can carry out after 5 hours
Seeds Germination Tests.
Each germination culture dish is put into 20, the seed by Dressing, 30 culture dishes of each processing setting, and
The CuSO of a concentration of 55mg/L is added in culture dish4.5H2O solution 20ml add distilled water 5ml daily according to Germinating status.In intelligence
It can be tested under the conditions of growth cabinet, environmental condition and management are executed in strict accordance with the regulation of GB/T3545；In 25 DEG C of people
It is germinateed with filter paper constant temperature in work climate box.Often processing takes 400 seeds in defined different time sections in time to experiment at random
The germination percentage of material, germinating energy coleoptile length are counted.It calculates, is cultivating when germination standard is with the 1/2 of bud a length of seed length
The 3rd day and 7 days afterwards, calculate germinating energy and germination percentage.Germination index：(Dt is germination number of days to GI=∑s Gt/Dt, and Gt is on t
Germinative number).Vitality index：VI=GI × S (GI is germination index, and S is single plant seedling dry weight).As a result such as table 1.
Influence of 1 corn seed treatment of table to Course of Corn Seed Germination under copper stressful environmental condition
Growth cabinet Seed germination shows the CuSO of 55mg/L4Under Stress treatment, after the processing of seed dressing 1~10
The first seed embryo root long of jade 335 and Zheng Dan 958 and slightly, color is more yellow, root hair is intensive, grow fine；Plumule growth is very fast, and
It is more healthy and strong, it is significantly higher than control seed, sprouting basis has been established in the growth in this corn later stage.As can be seen from Table 1, with it is right
Photograph ratio, seed dressing 1~10 all have the effect for promoting Course of Corn Seed Germination in various degree, and the experiment effect of configuration is notable, warp
Treated that its germinating energy, germination percentage, germination index, vitality index and coleoptile length are all significantly higher than control for seed dressing 1~10.
This illustrates the CuSO of 55mg/L4.5H2Under O Stress treatments, after seed dressing 1~10 handles corn seed, kind is helped speed up
Sub- water swelling, breaking dormancy are completed to sprout emergence process.
Experiment 2：The influence that corn seed treatment accumulates maize root system under copper stressful environmental condition and Leaf Physiology and substance.
The uniform two kinds of maize genotypes seeds (first 335 and Zheng Dan 958 of jade) for choosing full seed, are studied in east
It is carried out in northern College of Horticulture of agriculture university laboratory.Seed dressing 1~10 made above is taken to carry out Dressing experiment, control
It does not dress seed, studies under different Cu Stress treatment, root characteristics parameter, root activity and the leaf photosynthesis for studying corn are special
The influence of property, Antioxidant Enzyme Systems.Specific practice is as follows.Treated that seed binds up with gauze is urged in 25 DEG C of moisturizings for seed dressing
Bud, seed are broadcast after showing money or valuables one carries unintentionally in vermiculite, and growth cabinet is positioned over.After 7d corn seedling is fixed on sponge and to be made in advance
In benzene plate, gently move into Hoagland nutrient solutions in, and illumination box continue culture (25 DEG C of temperature, light intensity are
4000lux, illumination 12h/d), 10 hydroponic boxes are each handled, analysis measurement is carried out to ensure to obtain enough seedling.Wait for seedling
When growing to two leaves wholeheartedly, in Hoagland nutrient solutions, according to nutrient solution volume, crop field actual environment is simulated, CuSO is set4It is dense
Degree is 55mg/L, with air pump venting in timing manner (40min/h), replaces nutrient solution within every two days.After culture 15 days, often processing chooses 8 plants
It takes its root system to carry out the measurement of root activity and Root Characteristics parameter, leaf photosynthesis and Antioxidant Enzyme Systems feature, includes mainly
Hepatocuprein (SOD) activity, peroxidase (POD) activity and the analysis measurement with malonaldehyde (MDA) content, 3 times
It repeats.As a result such as table 2, table 3 and table 4.Using SPAD-502 types chlorophyll meter (Japanese MINLLTA companies production) measurement function leaf
The chlorophyll content (SPAD values) of piece and the fluorescence that WALZPAM-2100 chlorophyll fluorescence instrument measurement function blades are produced with Germany are joined
Number.After plant dry matter measurement takes 110 DEG C of water-removings of baking oven, dries 3 hours, weigh with scale.
Influence of 2 corn seed treatment of table to maize root system characteristic parameter and root activity under copper stressful environmental condition
Corn to the absorption of soil moisture and mineral element rely primarily on root system, root activity and Root Characteristics parameter from
It can reflect root system metabolic capability to a certain extent and resist the power of adverse circumstance ability.As can be seen from Table 2, copper stressful environmental condition
Under, by treated the corn seed Root Characteristics parameter of seed dressing 1~10, (root system overall length, root system total surface area, root system are overall
Product, average root diameter) and root activity be all remarkably higher than control.By seed dressing, treated that plant physically well develops,
Root system is healthy and strong, this can ensure that absorbability of the plant for moisture and nutrient, and preferable substance is provided for overground part leaf growth
It supports.
Influence of 3 corn seed treatment of table to maize leaf photosynthesis characteristics and dry-matter accumulation under copper stressful environmental condition
Note：The average value that seedling dry weight is 50 plants.
Chlorophyll (SPAD) height influences absorption and conversion of the maize leaf to luminous energy.Table 3 shows after seed dressing is handled
Corn can significantly improve Photosynthetic Characteristics of the blade under copper stressful environmental condition.Treated the blade of seed dressing 1~10
Middle SPAD values, Fv/Fm(the maximum light energy use efficiencies of PS II) and qN (non-photochemical quenching) are respectively higher than compareed.And F0 is (initial glimmering
Light) then it is substantially less than control.Seed dressing treated maize leaf SPAD values increase, and slow down copper stress to blade Photosystem I I
The injury of reaction center improves the ability of the anti-copper stress adverse circumstance of corn, improves the quantity of photosynthetic chain electron transmission, to
Be conducive to the output and transport of photosynthate.
Shadow of 4 corn seed treatment of table to maize leaf Antioxidant Enzyme Systems (SOD, POD, MDA) under copper stressful environmental condition
It rings
SOD in plant, POD and MDA are the important parameters of crop SCAVENGING SYSTEM OF ACTIVATED OXYGEN, can effectively remove free radical
And peroxide.Table 4 shows under copper stress conditions, and the Antioxidant Enzyme Systems parameter through seed dressing treated maize leaf changes
It is kind apparent.Superoxide dismutase (SOD) activity and peroxidase (POD) activity in treated the blade of seed dressing 1~10
Increase respectively than control, and malonaldehyde (MDA) content is reduced than control.This is to the membrane structure under protection copper stress conditions
Completely, copper stress can be slowed down to a certain extent preferably, to ensure the normal growth metabolism of plant, the corn of this and front
Photosynthetic characteristics and substance accumulation index performance are consistent.
The influence of embodiment 3, corn seed treatment to corn yield and its constituent element under copper stressful environmental condition.
It selects corn variety favorable to the people 33 that the first snow change of Heilungkiang is planted extensively and prosperous No. 2 is experiment material, it will be equal
The even corn seed for being mixed with seed dressing is on May 1st, 2013 and on April 28th, 2014 in Great Northern Wilderness organic fertilizer industry Co., Ltd reality
It carries out with testing, nearby belongs to the vaccary in Wandashan Mountains, Heilongjiang Province dairy industry base, because of the reason of feed contains copper in cultivation,
The heavy metal-polluted soil in experiment plot nearby, especially copper coin cellulose content are exceeded serious, after measured field Cu2+It is a concentration of
45.33mg/L.Seed dressing 5 is set and seed dressing 8 carries out field experiment, check experiment is done with uncoated corn seed (CK),
Per a length of 20m in ridge, row spacing 0.7m, 8 ridges are a cell, plot area 112m2, 6.25 ten thousand plants/hm of planting density2, at random
Block design repeats three times, amounts to 18 cells.According to Ministry of Agriculture's publication《Super High-yielding Maize production creates corn and surveys production scheme》Into
Row.
Influence (2013 year) of the 5 seed dressing embodiment of table to corn yield and its constituent element under copper stressful environmental condition
Table 5 shows under copper stress conditions, and through seed dressing, treated that corn maturity period yield is significantly higher than control.Implement
The ultimate output of example 8 is better than embodiment 1.Favorable to the people 33 increase than control respectively through seed dressing 8 and seed dressing 5 treated corn yield
Add yield 31.3% and 20.2%；Prosperous No. 2 increase than control respectively through seed dressing 8 and seed dressing 5 treated corn yield
Yield 29.8% and 16.8%, experiment show to handle by seed dressing, and the Northeast's copper will be made to coerce the plantation of soil corn
And its final high yield is possibly realized.
2014, We conducted repeating to test, the energy of seed dressing treated corn shows preferably anti-copper stress
Power.Ultimate output is shown as, and favorable to the people 33 increase yield than control respectively through seed dressing 8 and seed dressing 5 treated corn yield
26.23% and 22.1%；Prosperous No. 2 increase yield than control respectively through seed dressing 8 and seed dressing 5 treated corn yield
28.4% and 24.1%.
Since in Maize in Heilongjiang Province planting process, maize head smut has the tendency that further coming back, and is still corn
One of Major Diseases of production.General time incidence reaches 60%-70%, suffers heavy losses in 2%-8%, individual plot.We
During carrying out 2 years seed dressing field experiments, field investigation is found, is sent out by the maize head smut that seed dressing is handled
Sick rate is in 0.5%-0.8% or so, and the maize head smut incidence for compareing field illustrates in 4%-6% or so by mixing
Disease is controlled to a certain extent after kind agent Dressing, and technical support is provided for final corn with high yield stable yields.
The foregoing is merely presently preferred embodiments of the present invention, is merely illustrative for the purpose of the present invention, and not restrictive
's.Those skilled in the art understand that in the spirit and scope defined by the claims in the present invention many changes can be carried out to it,
It changes or even equivalent, but falls in protection scope of the present invention.
Claims (4)
1. a kind of anti-copper coerces corn seed treatment, it is characterised in that the seed dressing includes plant growth regulator, trace element, chela
Mixture, adhesive, wetting agent, colorant and water, wherein plant growth regulator by 2- (3,4- dichlorophenoxy) triethylamine,
γ-aminobutyric acid, benzene peptide amino acid and Prohexadione calcium composition, the trace element is by potassium citrate, zinc chloride, calcium nitrate and sulfuric acid
Manganese forms, and the chelating agent is ethylenediamine tetra-acetic acid, and described adhesive is Sodium Polyacrylate, and the wetting agent is detergent alkylate
Sodium sulfonate, the colorant are that dyestuff is red；Wherein the anti-copper coerces corn seed treatment by mass percentage by 0.02%-
2% 2- (3,4- dichlorophenoxy) triethylamine, the γ-aminobutyric acid of 0.01%-2%, 0.01%-1% benzene peptide amino acid,
The nitric acid of the Prohexadione calcium of 0.01%-2%, the potassium citrate of 0.05%-3%, the zinc chloride of 0.05%-3%, 0.05%-3%
Calcium, the manganese sulfate of 0.05%-3%, the ethylenediamine tetra-acetic acid of 0.5%-3%, the Sodium Polyacrylate of 0.2%-1%, 0.02%-
0.5% neopelex, the dyestuff of 0.05%-0.5% be red and the water of surplus composition.
2. the application of anti-copper stress corn seed treatment as described in claim 1, it is characterised in that concrete application method is：First to
Cold water is added in container, corn seed treatment is equably spread at a slow speed in water, Bian Sabian stirrings, after stirring 15 minutes, by corn
Seed pours into container, stirs evenly so that every the surface of the seed is in uniform color, and it is small then to spread naturally dry 4-5 out
When after can sow；Wherein the mass ratio of corn seed treatment and seed is 1：50, the mass ratio of corn seed treatment and water is 1：50,
The temperature of the cold water is 15~35 DEG C.
3. the application of anti-copper stress corn seed treatment according to claim 2, it is characterised in that prior to seeding by talcum powder
The corn seed mixing dressed seed, then sowed.
4. the application of anti-copper stress corn seed treatment according to claim 2, it is characterised in that the temperature of the cold water is
25℃。
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| CN106212459A (en) * | 2016-07-07 | 2016-12-14 | 东北农业大学 | Gamma aminobutyric acid strengthens maize under NaCl stress photosynthesis and the purposes of regulation and control endogenous hormones |
| CN107535492A (en) * | 2017-10-29 | 2018-01-05 | 惠州市无龄康态健康科技有限公司 | A kind of plant growth regualting composition containing benzene peptide amino acid and DCPTA |
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| CN109863855B (en) * | 2019-03-15 | 2021-05-25 | 中国计量大学 | Method for improving germination rate of rice seeds under cadmium stress |
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