CN105143443A - 微载体灌注培养方法及其用途 - Google Patents

微载体灌注培养方法及其用途 Download PDF

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Publication number
CN105143443A
CN105143443A CN201480022715.5A CN201480022715A CN105143443A CN 105143443 A CN105143443 A CN 105143443A CN 201480022715 A CN201480022715 A CN 201480022715A CN 105143443 A CN105143443 A CN 105143443A
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volume
liquid substratum
substratum
liquid
cell
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Chinese (zh)
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J·杨
Y·杨
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Genzyme Corp
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Genzyme Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
CN201480022715.5A 2013-02-22 2014-02-21 微载体灌注培养方法及其用途 Pending CN105143443A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361768215P 2013-02-22 2013-02-22
US61/768,215 2013-02-22
PCT/US2014/017785 WO2014130864A2 (fr) 2013-02-22 2014-02-21 Procédés de culture par perfusion de microporteurs et leurs utilisations

Publications (1)

Publication Number Publication Date
CN105143443A true CN105143443A (zh) 2015-12-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480022715.5A Pending CN105143443A (zh) 2013-02-22 2014-02-21 微载体灌注培养方法及其用途

Country Status (12)

Country Link
US (2) US10421949B2 (fr)
EP (2) EP2958989B1 (fr)
CN (1) CN105143443A (fr)
AU (1) AU2014218715B2 (fr)
CA (1) CA2901940A1 (fr)
DK (1) DK2958989T3 (fr)
ES (1) ES2717927T3 (fr)
HK (1) HK1219752A1 (fr)
HU (1) HUE042094T2 (fr)
MX (1) MX369522B (fr)
SG (1) SG11201506339RA (fr)
WO (1) WO2014130864A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107523534A (zh) * 2017-09-20 2017-12-29 北京亚东生物制药有限公司 重组cho细胞的贴壁微载体悬浮培养方法

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2014218723B2 (en) 2013-02-22 2019-12-05 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
AU2014218715B2 (en) 2013-02-22 2019-12-12 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
TW202204596A (zh) 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
JP6843750B2 (ja) 2014-12-22 2021-03-17 ジェンザイム・コーポレーション 哺乳類細胞の培養方法
CN116144500A (zh) 2016-04-14 2023-05-23 崔泽尔有限公司 具有恒流泵/管道系统的固定床生物反应器
EP3722435A1 (fr) 2016-04-14 2020-10-14 Trizell Ltd. Bioréacteur à lit fixe comportant un système de pompe/tube à débit constant
SG11202011296VA (en) 2018-05-15 2020-12-30 Voyager Therapeutics Inc Compositions and methods for the treatment of parkinson's disease
CN108866013B (zh) * 2018-08-06 2021-03-23 武汉赛科成科技有限公司 一种大规模生产慢病毒的方法
EP3911730A1 (fr) * 2019-01-16 2021-11-24 Corning Incorporated Systèmes et procédés de culture de cellules en suspension

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1373799A (zh) * 1999-09-03 2002-10-09 应用研究系统Ars股份公司 微载体培养中国仑鼠卵巢细胞生产异源性分泌蛋白质的方法
CN1564863A (zh) * 2001-10-02 2005-01-12 诺和诺德医疗保健公司 在真核细胞中生产重组蛋白的方法
WO2012152945A1 (fr) * 2011-05-12 2012-11-15 Probiogen Ag Amélioration du rendement de la production des protéines médiée par une gtpase de cdc42 à cyclage rapide

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
US6585971B1 (en) 1999-11-12 2003-07-01 Harbor-Ucla Research And Education Institute Recombinant α-L-iduronidase, methods for producing and purifying the same and methods for treating disease caused by deficiencies thereof
WO2002050251A2 (fr) 2000-12-20 2002-06-27 Bayer Pharmaceuticals Corporation Dispositif et procede d'expansion de trains de semences pour des cellules de mammiferes
AU2002348151A1 (en) 2001-11-05 2003-05-19 Genvec, Inc. Viral vector production methods and compositions
US6953692B2 (en) 2001-12-18 2005-10-11 Bayer Pharmaceuticals Corporation Device and method for seed-train expansion of mammalian cells
BE1016538A3 (fr) 2004-02-20 2007-01-09 Cesco Bioengineering Co Ltd Appareil et procede pour la preparation et la mise en culture de cellules.
WO2006033935A2 (fr) 2004-09-16 2006-03-30 Becton, Dickinson And Company Bioreacteur pour la culture de cellules sous perfusion
KR101238798B1 (ko) 2004-09-30 2013-03-04 바이엘 헬스케어, 엘엘씨 생물학적 분자의 통합된 연속 제조를 위한 장치 및 방법
ES2662589T3 (es) 2005-06-15 2018-04-09 Zhejiang Jinyishengshi Bioengineering Co., Ltd. Recipientes para cultivo en suspensión
WO2006138143A1 (fr) 2005-06-15 2006-12-28 Amprotein Corporation Flacon pour culture en suspension
WO2008024769A2 (fr) 2006-08-21 2008-02-28 Raven Biotechnologies, Inc. Procédé de production de perfusion intensifiée
US20080207487A1 (en) 2006-11-02 2008-08-28 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines
US8501462B2 (en) 2007-02-27 2013-08-06 The Board Of Trustees Of The University Of Illinois Insert device for multiwell plate
DK2155347T3 (da) 2007-04-17 2013-10-21 Xendo Holding B V Fremgangsmåde og apparat til kontinuerlig membranadsorption
US20090042253A1 (en) 2007-08-09 2009-02-12 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
WO2009034186A2 (fr) 2007-09-13 2009-03-19 Helmholtz-Zentrum für Infektionsforschung GmbH Procédé de culture cellulaire
US20090233334A1 (en) 2008-03-11 2009-09-17 Excellgene Sa Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale
DE102008019691A1 (de) 2008-04-15 2009-10-29 Technische Universität Ilmenau Teilaktives mikrofluidisches System für die 3D-Zellkultivierung sowie Verfahren zu dessen Perfusion
US20100076380A1 (en) 2008-06-16 2010-03-25 Amprotein Corporation Bioreactors
WO2010048417A2 (fr) 2008-10-22 2010-04-29 Biovest International, Inc. Bioréacteur à perfusion, systèmes de culture cellulaire et procédés de production de cellules et de produits dérivés de cellule
WO2010120514A2 (fr) 2009-03-31 2010-10-21 The Trustees Of The University Of Pennsylvania Protéines liant des antigènes comprenant des échafaudages protéiniques internes recombinants
EP2649016B1 (fr) 2010-12-06 2020-06-10 Pall Corporation Procédés de traitement continu pour produits biologiques
WO2013116449A1 (fr) 2012-02-02 2013-08-08 Corning Incorporated Consommables de microplaque de culture cellulaire à perfusion continue automatique
JP2015517804A (ja) 2012-04-01 2015-06-25 イー・エム・デイー・ミリポア・コーポレイシヨン 細胞培養及び傾斜移動アッセイ方法及び装置
SG10201901708SA (en) 2012-10-23 2019-03-28 Genzyme Corp Perfusion culturing methods and uses thereof
AU2014218723B2 (en) 2013-02-22 2019-12-05 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
AU2014218715B2 (en) 2013-02-22 2019-12-12 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
CN105377874A (zh) 2013-03-08 2016-03-02 建新公司 治疗性蛋白药物物质的整合连续制造
MX369521B (es) 2013-03-15 2019-11-11 Genzyme Corp Metodos de creacion de bancos de celulas de alta densidad.
DK3047013T3 (da) 2013-09-16 2021-11-15 Genzyme Corp Fremgangsmåder og systemer til forarbejdning af en cellekultur
TWI671312B (zh) 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
SG10202113019XA (en) 2014-06-04 2021-12-30 Amgen Inc Methods for harvesting mammalian cell cultures
TW202204596A (zh) 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
CN104480897B (zh) 2014-11-28 2017-01-04 上海市政工程设计研究总院（集团）有限公司 陆域占地少应用范围广的防汛墙
JP6843750B2 (ja) 2014-12-22 2021-03-17 ジェンザイム・コーポレーション 哺乳類細胞の培養方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1373799A (zh) * 1999-09-03 2002-10-09 应用研究系统Ars股份公司 微载体培养中国仑鼠卵巢细胞生产异源性分泌蛋白质的方法
CN1564863A (zh) * 2001-10-02 2005-01-12 诺和诺德医疗保健公司 在真核细胞中生产重组蛋白的方法
WO2012152945A1 (fr) * 2011-05-12 2012-11-15 Probiogen Ag Amélioration du rendement de la production des protéines médiée par une gtpase de cdc42 à cyclage rapide

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANA RITA COSTA ET AL.: "The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody", 《SPRINGERPLUS》 *
BEUM JUN KIM ET AL.: "Batch, Fed-Batch, and Microcarrier Cultures With CHO Cell Lines in a Pressure-Cycle Driven Miniaturized Bioreactor", 《BIOTECHNOLOGY AND BIOENGINEERING》 *
MARIA J.DE JESUS，ET AL.: "TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology", 《BIOCHEMICAL ENGINEERING JOURNAL》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107523534A (zh) * 2017-09-20 2017-12-29 北京亚东生物制药有限公司 重组cho细胞的贴壁微载体悬浮培养方法
CN107523534B (zh) * 2017-09-20 2020-10-02 北京亚东生物制药有限公司 重组cho细胞的贴壁微载体悬浮培养方法

Also Published As

Publication number Publication date
US20200080063A1 (en) 2020-03-12
US20160017291A1 (en) 2016-01-21
EP2958989A2 (fr) 2015-12-30
EP3460049A1 (fr) 2019-03-27
WO2014130864A3 (fr) 2014-10-16
MX369522B (es) 2019-11-11
ES2717927T3 (es) 2019-06-26
US10421949B2 (en) 2019-09-24
DK2958989T3 (en) 2019-03-25
CA2901940A1 (fr) 2014-08-28
AU2014218715A1 (en) 2015-09-10
AU2014218715B2 (en) 2019-12-12
HUE042094T2 (hu) 2019-06-28
WO2014130864A2 (fr) 2014-08-28
HK1219752A1 (zh) 2017-04-13
EP2958989B1 (fr) 2018-12-26
SG11201506339RA (en) 2015-09-29
MX2015010943A (es) 2016-06-07

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