CN105132491B - A kind of extracting method of pachymaran - Google Patents
A kind of extracting method of pachymaran Download PDFInfo
- Publication number
- CN105132491B CN105132491B CN201510695472.8A CN201510695472A CN105132491B CN 105132491 B CN105132491 B CN 105132491B CN 201510695472 A CN201510695472 A CN 201510695472A CN 105132491 B CN105132491 B CN 105132491B
- Authority
- CN
- China
- Prior art keywords
- added
- pachymaran
- alcohol
- time
- reactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention relates to a kind of extracting methods of pachymaran, the following steps are included: 4-6 times of water expansion is added in (1) after crushing Poria cocos, saccharomycete and lactic acid bacteria is added by 1%-5% (w/w), the two ratio is 1:2, it ferments, fermentation temperature is 28 DEG C -31 DEG C, PH3.1-4.0, time 12-16 hour；(2) fermentation liquid is uniformly mixed, complex enzyme formulation is added, which includes cellulase, hemicellulase, amylase and pectase, additive amount is 1.2%-2.5% (w/w), operative temperature is 36 DEG C -42 DEG C, PH3.5-4.2, and the time is 10-12 hours；(3) ethyl alcohol alcohol precipitation reactant, ethyl alcohol, which is added, makes reactant alcohol content reach 80% (w/w), by alcohol analysis object spray drying, can obtain water-soluble pachymaran 70.2% or so.The beneficial effects of the present invention are: extraction process is simple, the extraction process time shortens, and extracting factor is mild, and gained pachymaran content is high, bioactivity is strong, relative to existing extractive technique, has significant ground effect.
Description
Technical field
The present invention relates to the extractive technique field of polysaccharide, in particular to a kind of simple process, time is short, mild condition Fu
The extracting method of Siberian cocklebur polysaccharide.
Background technique
Poria cocos Poria cocos (Schw.) Wolf is the dry sclerotia of On Polyporaceae class, is traditional Chinese medicine.Begin to carry
It is cured in allusion and writes Shennong's Herbal, recorded in China's version over the years " Chinese Pharmacopoeia ", there is excreting dampness diuresis, stomach function regulating invigorating the spleen, calming heart
The effect of tranquilizing the mind；Modern pharmacology research show pachymaran have adjust immune function, anti-aging, antiallergy, it is antitumor,
The bioactivity such as anti-inflammatory.Pachymaran using very extensive, be not only can disease preventing and treating, but also can be in the dual-purpose of drug and food of health body-building
The big kind of medicine.
In the past few years, the extraction of pachymaran generally has Hot water extraction, lye extraction, acid system extraction, enzymatic isolation method etc.,
These methods are the runic objects for first obtaining pachymaran, then obtain the fine work of pachymaran using purifying process.Hot water leaching
Formulation extraction time is long and polysaccharide extract rate is low；Soda acid extraction extracting technology is cumbersome, and extracting condition is violent, is highly vulnerable to breakage polysaccharide
Stereochemical structure, make its bioactivity reduction；Enzymatic isolation method is at high cost, can only act on the substrate etc. of restriction, limit the wide of it
General application.
Therefore researching and developing the new pachymaran extracting method of one kind is field scientific research technical staff new issue anxious to be resolved
One of.
Summary of the invention
It is an object of the present invention to overcome the above deficiencies, provides that a kind of extraction process is simple, the time shortens, condition temperature
With the new method that gained pachymaran content is high, bioactivity is strong.
The present invention relates to a kind of extracting methods of pachymaran, comprising the following steps:
(1) 4-6 times of water expansion is added after crushing Poria cocos, saccharomycete and lactic acid bacteria is added by 1%-5% (w/w), carries out
Fermentation, fermentation temperature are 28 DEG C -31 DEG C, pH value 3.1-4.0, time 12-16 hour；
(2) fermentation liquid is uniformly mixed, complex enzyme formulation is added, which includes cellulase, hemicellulose
Enzyme, amylase and pectase, additive amount are 1.2%-2.5% (w/w), and operative temperature is 36 DEG C -42 DEG C, pH value 3.5-4.2,
Time is 10-12 hours；
(3) ethyl alcohol is added in the reactant after step (2), so that reactant alcohol content is reached 80% (w/w), later by alcohol
Object spray drying is analysed, water-soluble pachymaran is obtained.
On the basis of above scheme, the weight ratio of the saccharomycete and lactic acid bacteria is 1:2.
On the basis of above scheme, the weight of step (2) cellulase, hemicellulase, amylase and pectase
Than are as follows: 2:2:1:1.
On the basis of above scheme, concentration of alcohol used is 95% in step (3).
The beneficial effects of the present invention are: extraction process is simple, the extraction process time shortens, and extracting factor is mild, institute
It is strong to obtain pachymaran content height, bioactivity, relative to existing extractive technique, there is significant ground effect.The method of the present invention is one
A complete process, all linked with one another before and after all steps in method, any parameter in step is all by a large amount of real
It tests, any parameter is all the entire step of cooperation to realize final purpose, and change can all influence the final of entire method
As a result.
Specific embodiment
In conjunction with the preferred embodiment, to the specific embodiment provided according to the present invention, details are as follows:
Embodiment 1
A kind of extracting method of pachymaran: it carries out as follows: (every part based on 10g)
(1) 4 times of water expansions are added after crushing Poria cocos, saccharomycete is added by 1% (w/w) and lactic acid bacteria, the two ratio are
1:2 ferments, and fermentation temperature is 28 DEG C, pH value 3.1, and the time 12 hours；
(2) fermentation liquid is uniformly mixed, complex enzyme formulation is added, which includes cellulase, hemicellulose
Enzyme, amylase and pectase, the weight ratio of cellulase, hemicellulase, amylase and pectase are as follows: 2:2:1:1；Additive amount
For 1.2% (w/w), operative temperature is 36 DEG C, and pH value 3.5, the time is 10 hours；
(3) ethyl alcohol that concentration is 95% is added in the reactant after step (2), reactant alcohol content is made to reach 80%
(w/w), by alcohol analysis object spray drying, water-soluble pachymaran 70.1% or so can be obtained.
Embodiment 2
(1) 5 times of water expansions are added after crushing Poria cocos, saccharomycete and lactic acid bacteria is added by 3% (w/w), ferments, ferment
The weight ratio of female bacterium and lactic acid bacteria is 1:2, and fermentation temperature is 30 DEG C, pH value 3.5, the time 14 hours；
(2) fermentation liquid is uniformly mixed, complex enzyme formulation is added, which includes cellulase, hemicellulose
Enzyme, amylase and pectase, the weight ratio of cellulase, hemicellulase, amylase and pectase are as follows: 2:2:1:1；Additive amount
For 2% (w/w), operative temperature is 39 DEG C, and pH value 4, the time is 11 hours；
(3) ethyl alcohol that concentration is 95% is added in the reactant after step (2), reactant alcohol content is made to reach 80%
(w/w), alcohol analysis object spray drying is obtained into water-soluble pachymaran 70.2% later.
Embodiment 3
A kind of extracting method of pachymaran: it is characterized in that carrying out as follows: (every part based on 10g)
(1) 6 times of water expansions are added after crushing Poria cocos, saccharomycete is added by 5% (w/w) and lactic acid bacteria, the two ratio are
1:2 ferments, and fermentation temperature is 31 DEG C, pH value 4.0, and the time 16 hours；
(2) fermentation liquid is uniformly mixed, complex enzyme formulation is added, which includes cellulase, hemicellulose
Enzyme, amylase and pectase, the weight ratio of cellulase, hemicellulase, amylase and pectase are as follows: 2:2:1:1；Additive amount
For 2.5% (w/w), operative temperature is 42 DEG C, and pH value 4.2, the time is 12 hours；
(3) ethyl alcohol that concentration is 95% is added in the reactant after step (2), reactant alcohol content is made to reach 80%
(w/w), by alcohol analysis object spray drying, water-soluble pachymaran 70.15% can be obtained.
Clinical test:
The bioactivity of pachymaran is indicated with immunological enhancement of the pachymaran to domestic animal body.
Clinical trial animal selects 50 age in days pig 120.Individually raising is isolated in pig, feeds complete feed, and divide at random
It is 6 groups, every group 20,6 groups of feeding environments are identical, and experimental period carries out 7 days altogether；1 group is blank control group, is not done any
Processing；2 groups are drug control group, are pachymaran obtained by lye extraction；3 groups are drug control group, for acid solution extraction institute
Obtain pachymaran；4 groups are drug control group, are pachymaran obtained by Hot water extraction；5 groups are drug control group, are enzymatic isolation method
Gained pachymaran；6 groups are present composition group, and product of the present invention (composition obtained by the embodiment of the present invention 2) is fed
Pig is eaten, 4 drug control groups are identical with dosage used in present composition group, one time a day；It is inhaled after oral by gastrointestinal tract
Receipts achieve the purpose that improve immunity.
Test index: bone-marrow-derived lymphocyte EAC rosette rate=bone-marrow-derived lymphocyte EAC number positive/total number of cells × 100%, T lymph
Cell ANAE positive rate=T lymphocyte ANAE number positive/total number of cells × 100%.
Influence % of the table 1 to pig bone-marrow-derived lymphocyte EAC rosette rate
Statistical analysis, of the present invention group of bone-marrow-derived lymphocyte EAC garland formation rate is extremely significant to be higher than each drug control group and sky
White control group (P < 0.01) illustrates in terms of improving bone-marrow-derived lymphocyte EAC garland formation rate, present composition significant effect.
Influence % of the table 2 to pig T lymphocyte ANAE rosette rate
Statistical analysis, present composition group is extremely significant to T lymphocyte ANAE garland formation rate to be higher than each drug pair
According to group and blank control group (P < 0.01), illustrate in terms of improving lymphocyte ANAE garland formation rate, present composition group
Significant effect.
The detailed description that the extracting method of above-mentioned pachymaran carries out, is illustrative without being restrictive, can be according to
Limited range enumerates several embodiments, therefore the change and modification in the case where not departing from present general inventive concept, should belong to this
Within the protection scope of invention.
Claims (2)
1. a kind of extracting method of pachymaran, which comprises the following steps:
(1) 4-6 times of water expansion is added after crushing Poria cocos, saccharomycete and lactic acid bacteria is added by 1%-5% (w/w), ferments,
Fermentation temperature is 28 DEG C -31 DEG C, pH value 3.1-4.0, time 12-16 hour；
(2) fermentation liquid is uniformly mixed, complex enzyme formulation is added, which includes cellulase, hemicellulase, shallow lake
Powder enzyme and pectase, additive amount are 1.2%-2.5% (w/w), and operative temperature is 36 DEG C -42 DEG C, pH value 3.5-4.2, the time
It is 10-12 hours；
(3) ethyl alcohol is added in the reactant after step (2), so that reactant alcohol content is reached 80% (w/w), alcohol is analysed into object later
Spray drying, obtains water-soluble pachymaran；
The weight ratio of saccharomycete and lactic acid bacteria is 1:2 in the step (1)；
The weight ratio of step (2) cellulase, hemicellulase, amylase and pectase are as follows: 2:2:1:1；
Concentration of alcohol used is 95% in the step (3).
2. the extracting method of pachymaran according to claim 1, which comprises the following steps:
(1) 5 times of water expansions are added after crushing Poria cocos, saccharomycete and lactic acid bacteria is added by 3% (w/w), ferments, saccharomycete
Weight ratio with lactic acid bacteria is 1:2, and fermentation temperature is 30 DEG C, pH value 3.5, the time 14 hours；
(2) fermentation liquid is uniformly mixed, complex enzyme formulation is added, which includes cellulase, hemicellulase, shallow lake
Powder enzyme and pectase, the weight ratio of cellulase, hemicellulase, amylase and pectase are as follows: 2:2:1:1；Additive amount is 2%
(w/w), operative temperature is 39 DEG C, pH value 4, and the time is 11 hours；
(3) ethyl alcohol that concentration is 95% is added in the reactant after step (2), reactant alcohol content is made to reach 80% (w/w),
Later by alcohol analysis object spray drying, water-soluble pachymaran is obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510695472.8A CN105132491B (en) | 2015-10-23 | 2015-10-23 | A kind of extracting method of pachymaran |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510695472.8A CN105132491B (en) | 2015-10-23 | 2015-10-23 | A kind of extracting method of pachymaran |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105132491A CN105132491A (en) | 2015-12-09 |
| CN105132491B true CN105132491B (en) | 2018-12-28 |
Family
ID=54718058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510695472.8A Active CN105132491B (en) | 2015-10-23 | 2015-10-23 | A kind of extracting method of pachymaran |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105132491B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859902A (en) * | 2016-04-28 | 2016-08-17 | 武汉格罗宁根医药科技有限公司 | Water-soluble pachymaran metal complex and preparing method and application thereof |
| CN107890439B (en) * | 2017-11-15 | 2021-04-27 | 惠州兆婷化妆品有限公司 | Extraction method of aesculus hippocastanum seed extract and hair washing composition thereof |
| CN109200119A (en) * | 2018-11-23 | 2019-01-15 | 武汉德益华创科技有限责任公司 | Poria cocos fine powder preparation and preparation method thereof for treating childrens respiratory tract infection |
| TWI724352B (en) * | 2018-12-14 | 2021-04-11 | 大漢酵素生物科技股份有限公司 | Polysaccharide fermentation compositioncapable of anti-cancer, anti-virus, anti-inflammatory, promoting osteoblast proliferation, promoting intestinal stem cell proliferation effects and preparation method thereof. |
| CN111304266A (en) * | 2019-12-12 | 2020-06-19 | 武汉新华扬生物股份有限公司 | Biological enzymolysis fermentation process for aloe |
| CN112704712B (en) * | 2021-02-23 | 2022-02-08 | 马应龙药业集团股份有限公司 | Traditional Chinese medicine composition for eye care and preparation method and application thereof |
| CN114431401B (en) * | 2022-02-28 | 2023-07-04 | 江西省农业科学院农产品加工研究所 | Preparation method of asparagus polysaccharide emulsion and application of asparagus polysaccharide emulsion in rice flour aging resistance |
| CN115737709B (en) * | 2022-09-08 | 2024-01-26 | 高文红 | Biological fermentation product and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1339317A (en) * | 2000-08-21 | 2002-03-13 | 山东天赋生物保健品有限公司 | Poria oral liquor and its producing method |
| CN104643032A (en) * | 2015-02-04 | 2015-05-27 | 北京福原环球生物科技有限公司 | Extraction method of active ingredients of medicinal fungi |
| CN104961839A (en) * | 2015-06-12 | 2015-10-07 | 安徽华润金蟾药业股份有限公司 | Preparation method of specific pachyman formula granule |
-
2015
- 2015-10-23 CN CN201510695472.8A patent/CN105132491B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1339317A (en) * | 2000-08-21 | 2002-03-13 | 山东天赋生物保健品有限公司 | Poria oral liquor and its producing method |
| CN104643032A (en) * | 2015-02-04 | 2015-05-27 | 北京福原环球生物科技有限公司 | Extraction method of active ingredients of medicinal fungi |
| CN104961839A (en) * | 2015-06-12 | 2015-10-07 | 安徽华润金蟾药业股份有限公司 | Preparation method of specific pachyman formula granule |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105132491A (en) | 2015-12-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105132491B (en) | A kind of extracting method of pachymaran | |
| Zhou et al. | Applied modern biotechnology for cultivation of Ganoderma and development of their products | |
| CN104222674B (en) | The preparation method of a kind of channel catfish Chinese medicine immunity enhancer and application | |
| CN106360040B (en) | Composite Chinese herbal medicine probiotic feed additive for enhancing growth performance and immune function of broiler chickens | |
| CN105053593A (en) | Traditional Chinese medicine residues prepared poultry feed additive and preparation method thereof | |
| CN104623122B (en) | A kind of biological agent and preparation method thereof with anti-fatigue effect | |
| US20170347689A1 (en) | A probiotic-fermented maca composition and a method for preparing the composition and use of the composition | |
| CN104739919B (en) | A kind of fermentation Radix Astragali compound formulation and the preparation method and application thereof | |
| CN104013657A (en) | Post-fermentation extracting method of extracting saponin from American ginseng medicinal material | |
| CN104068379B (en) | A kind of health care oral liquid | |
| CN101138576B (en) | Codonopsis pilosula fermentation liquor and uses thereof | |
| CN103005466B (en) | Application for drone pupae polypeptide extract | |
| CN103756916A (en) | Acremonium terricola mutant strain and application thereof | |
| CN104186746A (en) | Preparation method of strain fermented tea | |
| CN104187436A (en) | Preparation method of meat rabbit feed additive obtained through fermentation of seaweed and Chinese herbal medicines | |
| CN102614499A (en) | Yak skin collagen compound traditional Tibetan medicine with anti-fatigue effect and preparation process thereof | |
| CN106666101A (en) | Feed additive prepared from fermented tea residues and application thereof | |
| CN103976351A (en) | Health food capable of enhancing immunity and improving sleep and two-step fermentation preparation method thereof | |
| CN103948023B (en) | The health food of a kind of develop immunitypty and improving water flood and two-step fermentation preparation method thereof | |
| KR101171942B1 (en) | Functional feed additive including ginseng by-product as active component | |
| CN101613652A (en) | Tuckahoe health care white spirit and preparation method thereof | |
| CN105559068A (en) | Composition obtained by fermenting tetrastigma hemsleyanum diels et gilg. through edible and medicinal fungi and preparation method of composition | |
| CN107118997B (en) | A kind of feeding clostridium butyricum, the probiotics for promoting growth of animals or poultry and preparation method thereof | |
| CN111449239B (en) | Functional food additive of ganoderma lucidum fermented sea buckthorn seed meal and preparation method thereof | |
| CN106071303A (en) | A kind of egg feedstuff containing lactic acid bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: An extraction method of Poria cocos polysaccharide Effective date of registration: 20210425 Granted publication date: 20181228 Pledgee: Qilu bank Limited by Share Ltd. Ji'nan Changqing branch Pledgor: SHANDONG SOOCOM ANIMAL REMEDY Co.,Ltd. Registration number: Y2021980002985 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220711 Granted publication date: 20181228 Pledgee: Qilu bank Limited by Share Ltd. Ji'nan Changqing branch Pledgor: SHANDONG SOOCOM ANIMAL REMEDY CO.,LTD. Registration number: Y2021980002985 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |