CN111304266A - Biological enzymolysis fermentation process for aloe - Google Patents
Biological enzymolysis fermentation process for aloe Download PDFInfo
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- CN111304266A CN111304266A CN201911276583.XA CN201911276583A CN111304266A CN 111304266 A CN111304266 A CN 111304266A CN 201911276583 A CN201911276583 A CN 201911276583A CN 111304266 A CN111304266 A CN 111304266A
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- 241001116389 Aloe Species 0.000 title claims abstract description 138
- 235000011399 aloe vera Nutrition 0.000 title claims abstract description 138
- 238000000855 fermentation Methods 0.000 title claims abstract description 22
- 230000004151 fermentation Effects 0.000 title claims abstract description 22
- 239000000843 powder Substances 0.000 claims abstract description 168
- 238000004880 explosion Methods 0.000 claims abstract description 38
- 108090000790 Enzymes Proteins 0.000 claims abstract description 15
- 102000004190 Enzymes Human genes 0.000 claims abstract description 15
- 238000000643 oven drying Methods 0.000 claims abstract description 12
- 238000010298 pulverizing process Methods 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 12
- 238000005303 weighing Methods 0.000 claims abstract description 12
- 238000004108 freeze drying Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- 241000186660 Lactobacillus Species 0.000 claims abstract description 7
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 7
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 239000000284 extract Substances 0.000 claims abstract description 4
- 102100032487 Beta-mannosidase Human genes 0.000 claims description 27
- 108010055059 beta-Mannosidase Proteins 0.000 claims description 27
- 108010059892 Cellulase Proteins 0.000 claims description 15
- 108010059820 Polygalacturonase Proteins 0.000 claims description 15
- 229940106157 cellulase Drugs 0.000 claims description 15
- 229940088598 enzyme Drugs 0.000 claims description 15
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 3
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 238000000605 extraction Methods 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 239000004310 lactic acid Substances 0.000 description 5
- YDQWDHRMZQUTBA-UHFFFAOYSA-N Aloe emodin Chemical compound C1=CC=C2C(=O)C3=CC(CO)=CC(O)=C3C(=O)C2=C1O YDQWDHRMZQUTBA-UHFFFAOYSA-N 0.000 description 4
- 229920002324 Galactoglucomannan Polymers 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- AFHJQYHRLPMKHU-XXWVOBANSA-N Aloin Natural products O=C1c2c(O)cc(CO)cc2[C@H]([C@H]2[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O2)c2c1c(O)ccc2 AFHJQYHRLPMKHU-XXWVOBANSA-N 0.000 description 3
- 241000235342 Saccharomycetes Species 0.000 description 3
- AFHJQYHRLPMKHU-OSYMLPPYSA-N aloin A Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1[C@@H]1C2=CC(CO)=CC(O)=C2C(=O)C2=C(O)C=CC=C21 AFHJQYHRLPMKHU-OSYMLPPYSA-N 0.000 description 3
- 239000000413 hydrolysate Substances 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- AFHJQYHRLPMKHU-UHFFFAOYSA-N isobarbaloin Natural products OC1C(O)C(O)C(CO)OC1C1C2=CC(CO)=CC(O)=C2C(=O)C2=C(O)C=CC=C21 AFHJQYHRLPMKHU-UHFFFAOYSA-N 0.000 description 3
- 229940098465 tincture Drugs 0.000 description 3
- 229920000057 Mannan Polymers 0.000 description 2
- CPUHNROBVJNNPW-UHFFFAOYSA-N aloin A Natural products OC1C(O)C(O)C(CO)OC1OC1C2=CC(CO)=CC(O)=C2C(=O)C2=C(O)C=CC=C21 CPUHNROBVJNNPW-UHFFFAOYSA-N 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- LQGUBLBATBMXHT-UHFFFAOYSA-N chrysophanol Chemical compound C1=CC=C2C(=O)C3=CC(C)=CC(O)=C3C(=O)C2=C1O LQGUBLBATBMXHT-UHFFFAOYSA-N 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- HKIKAXXIWJHWLY-ZIIYPAMZSA-N Aloesin Chemical compound C=12OC(CC(=O)C)=CC(=O)C2=C(C)C=C(O)C=1[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HKIKAXXIWJHWLY-ZIIYPAMZSA-N 0.000 description 1
- HKIKAXXIWJHWLY-QEVGBQTESA-N Aloesin Natural products O=C(CC=1Oc2c([C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)c(O)cc(C)c2C(=O)C=1)C HKIKAXXIWJHWLY-QEVGBQTESA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 241000219061 Rheum Species 0.000 description 1
- 235000009411 Rheum rhabarbarum Nutrition 0.000 description 1
- 229940006091 aloe polysaccharide Drugs 0.000 description 1
- KFJNVVJUICKJEQ-LQDZTQBFSA-N aloenin Chemical compound O1C(=O)C=C(OC)C=C1C1=C(C)C=C(O)C=C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 KFJNVVJUICKJEQ-LQDZTQBFSA-N 0.000 description 1
- KFJNVVJUICKJEQ-UHFFFAOYSA-N aloenin Natural products O1C(=O)C=C(OC)C=C1C1=C(C)C=C(O)C=C1OC1C(O)C(O)C(O)C(CO)O1 KFJNVVJUICKJEQ-UHFFFAOYSA-N 0.000 description 1
- -1 alomiac tincture Chemical compound 0.000 description 1
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 238000005422 blasting Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- NZPQWZZXRKZCDU-UHFFFAOYSA-N chrysophanol Natural products Cc1cc(O)c2C(=O)c3c(O)cccc3Oc2c1 NZPQWZZXRKZCDU-UHFFFAOYSA-N 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 238000005325 percolation Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 239000011345 viscous material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- Life Sciences & Earth Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- General Chemical & Material Sciences (AREA)
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Abstract
The invention provides a biological enzymolysis fermentation process for aloe, which comprises the following steps: pulverizing fresh folium Aloe with pulverizer, and oven drying to obtain folium Aloe powder sample; weighing a proper amount of aloe leaf powder sample, and performing steam explosion treatment on the aloe leaf powder sample for a first preset time, wherein the steam explosion temperature is a first preset temperature; adding biological enzyme powder into the aloe leaf powder sample subjected to steam explosion to obtain an enzymolysis solution, and reducing the temperature of the enzymolysis solution to a second preset temperature; continuously extracting the obtained enzymolysis liquid at a third preset temperature for a second preset time to obtain an extracting solution and collecting the extracting solution; cooling the obtained enzymolysis liquid to a fourth preset temperature, adding lactobacillus and/or yeast for a third preset time, collecting the extract, freeze-drying to obtain powder, bagging and sealing.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of fermentation, in particular to a biological enzymolysis and fermentation process for aloe.
[ background of the invention ]
The aloe active ingredient is mainly aloe anthraquinone complex and aloe polysaccharide, which are highly active phenolic compounds, and are the main constituent elements of aloe leaf exudate, including more than 20 kinds of aloin, aloe tincture, aloesin, aloenin, alourrine, alohuangxin, aloxanthin, aloin A, alomiac tincture, aloe emodin, aloe rhubarb glycoside, isoaloin, aloe chrysophanol, etc. Aloe-emodin, aloe-chrysophanol and barbaloin have good effects in invigorating stomach, inhibiting bacteria, relieving inflammation and purgation.
Aloe tincture has antiviral and antiinflammatory effects; the aloe saccharide substance is the main component of mucus in aloe liquid, and the viscous substance in mesophyll is mannan, which has obvious effect on improving animal immunity. The aloe has complex and various nutrient components, main nutrient components are easy to dissolve in water, the water is used as a solvent, and the increase of the water-soluble components in the aloe extracting solution can fully utilize the effective components of the aloe, thereby better exerting the efficacy of the aloe.
At present, the extraction method of aloe mainly comprises the following steps: water decoction, reflux extraction, immersion, percolation, ultrasonic extraction, etc. These methods can be performed under laboratory conditions, but the existing extraction methods of aloe do not fully utilize the active ingredients of aloe.
In view of the above, there is a need to provide a new bio-enzymatic fermentation process for aloe to overcome the above-mentioned drawbacks.
[ summary of the invention ]
The invention aims to provide a biological enzymolysis and fermentation process for aloe, which can fully utilize the active ingredients of the aloe and has an unobvious bacteriostatic effect.
In order to achieve the above object, the present invention provides a biological enzymolysis fermentation process for aloe, comprising the following steps:
pulverizing fresh folium Aloe with pulverizer, and oven drying to obtain folium Aloe powder sample;
weighing a proper amount of aloe leaf powder sample, and performing steam explosion treatment on the aloe leaf powder sample for a first preset time, wherein the steam explosion temperature is a first preset temperature; adding biological enzyme powder into the aloe leaf powder sample subjected to steam explosion to obtain an enzymolysis solution, and reducing the temperature of the enzymolysis solution to a second preset temperature;
continuously extracting the obtained enzymolysis liquid at a third preset temperature for a second preset time to obtain an extracting solution and collecting the extracting solution;
cooling the obtained enzymolysis liquid to a fourth preset temperature, adding lactobacillus and/or yeast for a third preset time, collecting the extract, freeze-drying to obtain powder, bagging and sealing.
Specifically, the biological enzyme powder is any one or more of cellulase powder, mannanase powder and pectinase powder.
Specifically, the steam explosion treatment time is as follows: 3-30min, wherein the first preset temperature range is as follows: 170 ℃ and 250 ℃, wherein the second preset temperature range is as follows: 50-55 ℃.
Specifically, the moisture content of the aloe leaf powder sample after steam explosion treatment is as follows: 20 to 80 percent, wherein the addition amount of the biological enzyme powder is 0.5 to 5 percent of the mass of the aloe leaf powder sample.
Specifically, the moisture content of the aloe leaf powder sample after steam explosion treatment is as follows: 70 percent.
Specifically, the third preset temperature range is as follows: 65-75 ℃; the second preset time range is as follows: 2-8 hours.
Specifically, the addition amount of the lactobacillus and/or the yeast is 5 per mill-3% of the mass of the aloe leaf powder sample.
Specifically, the range of the fourth preset temperature is as follows: 28-37 ℃; the third preset time range is as follows: 16-48 h.
Compared with the prior art, the biological enzymolysis and fermentation process for the aloe has the advantages that the aloe leaf powder can permeate into cell walls of the aloe leaf powder through steam explosion treatment, the structure of galactoglucomannan components in the aloe leaf powder is damaged, the galactoglucomannan in the aloe leaf can be easily combined with enzymes such as mannase and degraded by the enzymes, more mannooligosaccharides are obtained to achieve a better antibacterial effect, the biological enzymolysis and fermentation process has the function of improving immunity, and the effective components of the aloe are fully utilized.
[ detailed description ] embodiments
In order to make the objects, technical solutions and advantageous effects of the present invention more apparent, the present invention is further described in detail with reference to the following detailed description. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
The invention provides a biological enzymolysis and fermentation process for aloe, which comprises the following steps:
pulverizing fresh folium Aloe with pulverizer, and oven drying to obtain folium Aloe powder sample;
weighing a proper amount of aloe leaf powder sample, and performing steam explosion treatment on the aloe leaf powder sample for a first preset time, wherein the steam explosion temperature is a first preset temperature; adding biological enzyme powder into the aloe leaf powder sample subjected to steam explosion to obtain an enzymolysis solution, and reducing the temperature of the enzymolysis solution to a second preset temperature;
continuously extracting the obtained enzymolysis liquid at a third preset temperature for a second preset time to obtain an extracting solution and collecting the extracting solution;
cooling the obtained enzymolysis liquid to a fourth preset temperature, adding lactobacillus and/or yeast for a third preset time, collecting the extract, freeze-drying to obtain powder, bagging and sealing.
Specifically, the biological enzyme powder is any one or more of cellulase powder, mannanase powder and pectinase powder.
Specifically, the steam explosion treatment time is as follows: 3-30min, wherein the first preset temperature range is as follows: 170 ℃ and 250 ℃, wherein the second preset temperature range is as follows: 50-55 ℃.
Specifically, the moisture content of the aloe leaf powder sample after steam explosion treatment is as follows: 20 to 80 percent, wherein the addition amount of the biological enzyme powder is 0.5 to 5 percent of the mass of the aloe leaf powder sample.
Specifically, the moisture content of the aloe leaf powder sample after steam explosion treatment is as follows: 70 percent.
Specifically, the third preset temperature range is as follows: 65-75 ℃; the second preset time range is as follows: 2-8 hours.
Specifically, the addition amount of the lactobacillus and/or the yeast is 5 per mill-3% of the mass of the aloe leaf powder sample.
Specifically, the range of the fourth preset temperature is as follows: 28-37 ℃; the third preset time range is as follows: 16-48 h.
Comparative example (conventional bio-enzymolysed aloe):
pulverizing fresh folium Aloe with pulverizer, oven drying to obtain folium Aloe powder sample; weighing a proper amount of aloe leaf powder sample, mixing the aloe leaf powder sample with cellulase powder, pectinase powder and mannase powder, adding water into the mixed aloe leaf powder sample, pectinase powder and mannase powder to enable the water content of the mixed aloe leaf powder sample, pectinase powder and mannase powder to be 70%, and reducing the temperature of enzymolysis liquid to 55 ℃, wherein the adding amount of the cellulase powder is 1% of the mass of the aloe leaf powder sample, the adding amount of the pectinase powder is 1% of the mass of the aloe leaf powder sample, and the adding amount of the mannase powder is 1% of the mass of the aloe leaf powder sample; extracting the obtained enzymolysis solution at 60 deg.C for 8 hr, collecting extractive solution, freeze drying the extractive solution to obtain powder, bagging, and sealing.
Example 1:
pulverizing fresh folium Aloe with pulverizer, oven drying to obtain folium Aloe powder sample; weighing appropriate amount of folium Aloe powder sample, and performing steam explosion at 210 deg.C for 12.5 min; adding pectinase powder into the aloe leaf powder sample to obtain an enzymolysis solution, reducing the temperature of the enzymolysis solution to 55 ℃, keeping the moisture content of the aloe leaf powder sample after steam explosion at 70%, wherein the addition amount of the pectinase powder is 3% of the mass of the aloe leaf powder sample, continuously extracting the obtained enzymolysis solution at 60 ℃ for 8 hours, collecting an extracting solution after extraction, freeze-drying the extracting solution to prepare powder, bagging and sealing.
Example 2:
pulverizing fresh folium Aloe with pulverizer, oven drying to obtain folium Aloe powder sample; weighing appropriate amount of folium Aloe powder sample, and performing steam explosion at 210 deg.C for 12.5 min; adding cellulase powder into the aloe leaf powder sample to obtain an enzymolysis solution, reducing the temperature of the enzymolysis solution to 55 ℃, continuously extracting the obtained enzymolysis solution at 60 ℃ for 8 hours, collecting the extracting solution after the extraction is finished, freeze-drying the extracting solution to prepare powder, bagging and sealing, wherein the water content of the aloe leaf powder sample after steam explosion is 70%, and the adding amount of the cellulase powder is 3% of the mass of the aloe leaf powder sample.
Example 3:
pulverizing fresh folium Aloe with pulverizer, oven drying to obtain folium Aloe powder sample; weighing appropriate amount of folium Aloe powder sample, and performing steam explosion at 210 deg.C for 12.5 min; adding mannase powder into folium Aloe powder sample to obtain enzymolysis solution, cooling to 55 deg.C, steam blasting to obtain folium Aloe powder sample with water content of 70%, wherein the addition amount of mannase powder is 3% of folium Aloe powder, extracting the obtained enzymolysis solution at 60 deg.C for 8 hr, collecting extractive solution, freeze drying the extractive solution to obtain powder, bagging, and sealing.
Example 4:
pulverizing fresh folium Aloe with pulverizer, oven drying to obtain folium Aloe powder sample; weighing appropriate amount of folium Aloe powder sample, and performing steam explosion at 210 deg.C for 12.5 min; adding cellulase powder, pectinase powder and mannase powder into an aloe leaf powder sample to obtain an enzymolysis solution, cooling the temperature of the enzymolysis solution to 55 ℃, wherein the moisture content of the aloe leaf powder sample after steam explosion is 70%, the addition amount of the cellulase powder is 1% of the mass of the aloe leaf powder sample, the addition amount of the pectinase powder is 1% of the mass of the aloe leaf powder sample, the addition amount of the mannase powder is 1% of the mass of the aloe leaf powder sample, continuously extracting the obtained enzymolysis solution at 60 ℃ for 8 hours, collecting an extracting solution after extraction is finished, freezing and drying the obtained solution to prepare powder, bagging and sealing.
Example 5:
pulverizing fresh folium Aloe with pulverizer, oven drying to obtain folium Aloe powder sample; weighing appropriate amount of folium Aloe powder sample, and performing steam explosion for 12.5min at 210 deg.C; adding cellulase powder, pectinase powder and mannase powder into an aloe leaf powder sample to obtain an enzymolysis solution, reducing the temperature of the enzymolysis solution to 55 ℃, wherein the moisture content of the aloe leaf powder sample after steam explosion is 70%, the addition amount of the cellulase powder is 1% of the mass of the aloe leaf powder sample, the addition amount of the pectinase powder is 1% of the mass of the aloe leaf powder sample, the addition amount of the mannase powder is 1% of the mass of the aloe leaf powder sample, continuously extracting the obtained enzymolysis solution at 60 ℃ for 8 hours, reducing the temperature of the enzymolysis solution to 28 ℃ after extraction is finished, adding saccharomycetes, fermenting for 24 hours, wherein the addition amount of the saccharomycetes is 1% of the mass of the aloe leaf powder sample, collecting fermentation liquor, freeze-drying to prepare powder, bagging and sealing.
Example 6:
pulverizing fresh folium Aloe with pulverizer, oven drying to obtain folium Aloe powder sample; weighing appropriate amount of folium Aloe powder sample, and performing steam explosion at 210 deg.C for 12.5 min; adding cellulase powder into an aloe leaf powder sample to obtain an enzymatic hydrolysate, reducing the temperature of the enzymatic hydrolysate to 55 ℃, wherein the moisture content of the aloe leaf powder sample after steam explosion is 70%, the addition amount of the cellulase powder is 1% of the mass of the aloe leaf powder sample, the addition amount of the pectase powder is 1% of the mass of the aloe leaf powder sample, the addition amount of the mannan enzyme powder is 1% of the mass of the aloe leaf powder sample, continuously extracting the obtained enzymatic hydrolysate at 60 ℃ for 8 hours, reducing the temperature of the enzymatic hydrolysate to 28 ℃ after extraction is finished, adding lactic acid bacteria for fermentation for 24 hours, wherein the addition amount of the lactic acid bacteria is 1% of the mass of the aloe leaf powder sample, collecting fermentation liquor, freeze-drying to prepare powder, bagging and sealing.
Example 7:
pulverizing fresh folium Aloe with pulverizer, oven drying to obtain folium Aloe powder sample; weighing appropriate amount of folium Aloe powder sample, and performing steam explosion at 210 deg.C for 12.5 min; adding cellulase powder, pectinase powder and mannase powder into an aloe leaf powder sample to obtain an enzymolysis solution, reducing the temperature of the enzymolysis solution to 55 ℃, wherein the moisture content of the aloe leaf powder sample after steam explosion is 70%, the addition amount of the cellulase powder is 1% of the mass of the aloe leaf powder sample, the addition amount of the pectinase powder is 1% of the mass of the aloe leaf powder sample, the addition amount of the mannase powder is 1% of the mass of the aloe leaf powder sample, continuously extracting the obtained enzymolysis solution at 60 ℃ for 8 hours, reducing the temperature of the enzymolysis solution to 28 ℃ after extraction is finished, adding saccharomycetes and lactic acid bacteria, fermenting for 24 hours, wherein the addition amounts of saccharomyces cerevisiae and lactic acid bacteria are 1% of the mass of the aloe leaf powder sample, collecting fermentation liquor, freeze-drying to prepare powder, bagging and sealing.
Experimental data using the comparative example and examples 1-7 are shown in table 1 below,
table 1:
after the aloe leaf powder sample is treated by steam explosion, saturated steam can penetrate into cell walls of the aloe leaf powder sample and destroy the structure of the galactoglucomannan component in the aloe leaf powder sample, so that the galactoglucomannan in the aloe leaf can be easily combined with enzymes such as mannanase and the like and degraded by the enzymes.
As can be seen from Table 1, in each of examples 1-2, no mannanase powder was added, and in each of examples 3-7, mannanase powder was added, and the mannanase was effectively degraded into mannooligosaccharides by adding bio-enzymes, yeast and/or lactic acid bacteria, and the mannooligosaccharides obtained in examples 1-2 were significantly less than those obtained in examples 3-7; the mannanase powder is added in the comparative example, because the aloe leaf powder sample in the comparative example is not subjected to steam explosion treatment, the mannanase powder obtained in the comparative example 1 is obviously lower than the mannanase powder obtained in the examples 3-7, the dissolution rate of active ingredients of the aloe leaf powder sample subjected to enzymolysis fermentation and the yield of the mannanase powder are improved through steam explosion in the examples 3-7, the mannanase powder can play a role in inhibiting bacteria and has the function of improving immunity, and the more the mannanase powder sample obtained in the examples 3-7 is, the more the mannanase powder sample has the effect of inhibiting bacteria.
The invention is not limited solely to that described in the specification and embodiments, and additional advantages and modifications will readily occur to those skilled in the art, so that the invention is not limited to the specific details, representative apparatus, and examples shown and described herein, without departing from the spirit and scope of the general concept as defined by the appended claims and their equivalents.
Claims (8)
1. A biological enzymolysis fermentation process for aloe is characterized by comprising the following steps:
s1: pulverizing fresh folium Aloe with pulverizer, and oven drying to obtain folium Aloe powder sample;
s2: weighing a proper amount of aloe leaf powder sample, and performing steam explosion treatment on the aloe leaf powder sample for a first preset time, wherein the steam explosion temperature is a first preset temperature; adding biological enzyme powder into the aloe leaf powder sample subjected to steam explosion to obtain an enzymolysis solution, and reducing the temperature of the enzymolysis solution to a second preset temperature;
s3: continuously extracting the obtained enzymolysis liquid at a third preset temperature for a second preset time to obtain an extracting solution and collecting the extracting solution;
s4: cooling the obtained enzymolysis liquid to a fourth preset temperature, adding lactobacillus and/or yeast for a third preset time, collecting the extract, freeze-drying to obtain powder, bagging and sealing.
2. The process of claim 1, wherein the biological enzyme powder is any one or more of cellulase powder, mannanase powder and pectinase powder.
3. The bio-enzymatic fermentation process for aloe as claimed in claim 1, wherein the steam explosion treatment time is: 3-30min, wherein the first preset temperature range is as follows: 170 ℃ and 250 ℃, wherein the second preset temperature range is as follows: 50-55 ℃.
4. The bio-enzymatic fermentation process for aloe as claimed in claim 1, wherein the moisture content of the aloe leaf powder sample after steam explosion treatment is: 20 to 80 percent, wherein the addition amount of the biological enzyme powder is 0.5 to 5 percent of the mass of the aloe leaf powder sample.
5. The bio-enzymatic fermentation process for aloe as claimed in claim 4, wherein the moisture content of the aloe leaf powder sample after steam explosion treatment is: 70 percent.
6. The bio-enzymatic fermentation process for aloe as claimed in claim 1, wherein the third predetermined temperature range is: 65-75 ℃; the second preset time range is as follows: 2-8 hours.
7. The process of claim 1, wherein the amount of lactobacillus and/or yeast is 5% to 3% of the weight of the aloe leaf powder sample.
8. The bio-enzymatic fermentation process for aloe as claimed in claim 1, wherein the fourth predetermined temperature range is: 28-37 ℃; the third preset time range is as follows: 16-48 h.
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