CN105123762A - Sterilization composition containing Bacillus subtilis and prothioconazole and application thereof - Google Patents
Sterilization composition containing Bacillus subtilis and prothioconazole and application thereof Download PDFInfo
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Abstract
The invention discloses a sterilization composition containing Bacillus subtilis and prothioconazole and an application thereof. The composition is composed of a first active component Bacillus subtilis and a second active component prothioconazole and an auxiliary agent, the weight ratio between the first active component and the second active component is 1:50-50:1, and preferably is 1:30-30:1. The sum of the contents of the first active component and the second active component is 10-85% of the total weight of the composition, and preferably is 25-80%. The composition can be prepared into agriculturally permitted formulation, such as wettable powder, water dispersible granule, suspending agent, emulsion in water and suspoemulsion. The invention has the advantages of reasonable ingredients and good sterilization effect, which are not from simple addition of each ingredient activity, compared with the present single formulation, the sterilization composition provided by the invention has substantial sterilization effect, substantial synergic effect, and safety for crops. The sterilization composition provided by the invention has substantial control effects for crop powdery mildew and sheath blight.
Description
Technical field
The present invention relates to a kind of bactericidal composition, be made up of the first active component bacillus subtilis and the second active component prothioconazoles and auxiliary agent, belong to technical field of compounded pesticide.
Background technology
Bacillus subtilis, English name is bacillussubtilis, and being aerobic, the sporiferous Gram-positive bacillus of a class, is a kind of microbial bactericide, and bacterial classification is separated from soil or axis and obtains, and is brevibacterium.There is good preventive effect to powdery mildew, banded sclerotial blight, false smut, there is activation plant growth, alleviate the effect of the germ harm such as bacterial leaf streak of rice, bacterial leaf-blight, bakanae disease.
Prothioconazoles, English name is prothioconazole, chemical name is (RS)-2-[2-(1-chlorine cyclopropyl)-3-(2-chlorphenyl)-2-hydroxypropyl]-2,4-dihydro-1,2,4-triazole-3-thioketones, it is a kind of new triazolinones systemic fungicide, as a kind of demethylation inhibitors (DMI), play the precursor of sterol in Antifungi---lanosterol demethylation effect on 14-or-24 methylene lanostenol.It is active that this this active ingredient has fabulous protection, treatment and elimination.Be used for preventing and treating powdery mildew, banded sclerotial blight, rust, brown spot, leaf spot etc.
Applicant is through retrieval, bacillus subtilis and prothioconazoles mixture is not also had to prevent and treat the bibliographical information of powdery mildew, banded sclerotial blight at present, be further advanced by test to find, by bacillus subtilises different for the mechanism of action and prothioconazoles composite, there is obvious synergistic effect, the control efficiency to powdery mildew, banded sclerotial blight can be significantly improved, reduce dosage, reduce cost accounting, high to crop safety.
Summary of the invention
The object of the present invention is to provide a kind of component reasonable, synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, to the agricultural bactericidal composition containing bacillus subtilis and prothioconazoles of crop safety.
The bactericidal composition preparation formulation provided containing bacillus subtilis and prothioconazoles is provided.
Another object of the present invention is to provide above-mentioned composition preventing and treating the application in powdery mildew and banded sclerotial blight.
In order to overcome the defect of existing unitary agent, technical scheme of the present invention solves like this:
A) the first active component bacillus subtilis;
B) the second active component prothioconazoles;
The weight ratio of the first active component and the second active component is 1:50 ~ 50:1.
First active component content is 5% ~ 75% of described composition total weight, is preferably 10% ~ 70%; Second active component content is 5% ~ 55% of described composition total weight, is preferably 15% ~ 45%.
The content sum of the first active component and the second active component is 10% ~ 85% of described composition total weight, is preferably 25% ~ 80%.
A kind of bactericidal composition containing bacillus subtilis and prothioconazoles of the present invention is wetting powder, water dispersible granules, suspending agent, aqueous emulsion, suspension emulsion according to the preparation formulation that the method that those skilled in the art of the present technique are known can be prepared.
To wetting powder, spendable auxiliary agent has: dispersant is one or more in polycarboxylate, lignosulfonates, polyoxyethylene polyoxypropylene base ether block copolymers, alkyl naphthalene formaldehyde condensate sulfonates, alkylphenol-polyethenoxy base ether phosphate, aliphatic alcohol polyoxyvinethene phosphate, alkylphenol-polyethenoxy base ether sulfonate etc.; Wetting agent is one or more in alkyl sulfate, alkylsulfonate, alkylnaphthalene sulfonate etc.; Filler is one or more in ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay etc.
To water dispersible granules, spendable auxiliary agent has: dispersant is as one or more in polycarboxylate (TERSPERSE2700, T36, GY-D06 etc.), lignosulfonates, polyoxyethylene polyoxypropylene base ether block copolymers, alkyl naphthalene formaldehyde condensate sulfonates, alkylphenol-polyethenoxy base ether phosphate, aliphatic alcohol polyoxyvinethene phosphate, alkylphenol-polyethenoxy base ether sulfonate etc.; Wetting agent is as one or more in alkyl sulfate, alkylsulfonate, naphthalene sulfonate etc.; Disintegrant is as one or more in ammonium sulfate, sodium sulphate, urea, sodium bicarbonate, polyvinylpyrrolidone, starch and derivative, bentonite etc.; Binding agent is as one or more in starch, glucose, polyvinyl alcohol, polyethylene glycol, sodium carboxymethylcellulose, sucrose etc.; Filler is as one or more in diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay etc.
To suspending agent, spendable auxiliary agent has: dispersant is polycarboxylate, lignosulfonates, alkyl naphthalene formaldehyde condensate sulfonates, alkylphenol-polyethenoxy base ether phosphate, alkylphenol-polyethenoxy base ether sulfonate, phenethyl phenol polyethenoxy base ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, pull open in powder, alkylpolyoxyethylene phosphate etc. one or more; Wetting agent is in alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, alkylnaphthalene sulfonate, alkyl phenol formaldehyde resin polyoxyethylene ether, one or more in triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer etc.; Thickener is one or more in xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate, carboxymethyl cellulose, carboxyethyl cellulose, methylcellulose etc.; Preservative is one or more in formaldehyde, benzoic acid, Sodium Benzoate, potassium sorbate, isothiazolinone etc.; Defoamer is silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 aliphatic alcohols, hexanol, one or more in butanols, octanol etc.; Antifreezing agent is one or more in ethylene glycol, propane diols, glycerine, polyethylene glycol, sorbierite, urea, inorganic salts etc.; Water is deionized water.
To aqueous emulsion, spendable auxiliary agent has: emulsifier is as Nonyl pheno (EO=10) ether phosphate, triphenoethyl benzene phenol polyethenoxy ether phosphate, Nongru-700 #(common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201#, this dish-60#(common name: Span60), Tween-60 #(common name: polyoxyethylene sorbitan stearate), TX-10(common name: octyl phenol polyoxyethylene (10) ether), agriculture breast 1601(common name: triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer), agriculture breast 600#, one or more in agriculture breast 400# etc., solvent is as one or more in dimethylbenzene, toluene, cyclohexanone, solvent naphtha (trade mark: S-150, S-180, S-200) etc., stabilizing agent is as one or more in triphenyl phosphite, epoxychloropropane, epoxy soybean wet goods, antifreezing agent is if ethylene glycol, propane diols, glycerine, urea, inorganic salts are as one or more in sodium chloride etc., thickener is as one or more in xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate etc., preservative is as one or more in formaldehyde, benzoic acid, Sodium Benzoate etc., and water is deionized water.
To suspension emulsion, spendable auxiliary agent has: emulsifier as calcium dodecyl benzene sulfonate (agriculture breast 500#), Nongru-700 #, agriculture breast 2201, this coil-60#, emulsifier T-60(common name: anhydrous sorbitol single-hard ester acid ester APEO), TX-10(common name: octyl phenol polyoxyethylene (10) ether), agriculture breast 1601#, one or more in agriculture breast 600#, the newborn 400# of agriculture etc.; Dispersant is as one or more in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate etc.; Solvent is as one or more in repefral, dibutyl phthalate, ethyl acetate, methyl benzoate etc.; Defoamer is as one or more in silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 aliphatic alcohols, hexanol, butanols, octanol etc.; Thickener is as one or more in white carbon, polyvinyl alcohol, bentonite, aluminium-magnesium silicate etc.; Antifreezing agent is if ethylene glycol, propane diols, glycerine, urea, inorganic salts are as one or more in sodium chloride etc.; Water is deionized water.
Instant component is reasonable, and treatment adds protective effect, and bactericidal effect is good, and drug cost is low, and its activity and bactericidal effect are not the simple superposition of each composition activity, but has significant synergies, good to crop safety, meets the security requirement of pesticidal preparations.The present invention has good control efficiency to powdery mildew, banded sclerotial blight.
Embodiment
Measure below by mixture synergy and the synergistic effect to powdery mildew and banded sclerotial blight of bacillus subtilis and prothioconazoles mixture is described.
1, the synergy of wheat powdery mildew mixture is measured.
In order to prevent and treat the wheat powdery mildew in agricultural production, we have carried out mutual composite synergy with bacillus subtilis and prothioconazoles and have studied, and concrete grammar is as follows.
Subjects be wheat powdery mildew (
erysipegraminis).Former medicine is mixed with the test medicine of needs, test method is with reference to " farm-chemical indoor determination test rule bactericide the 4th part: control wheat powdery mildew test pot-culture method " and " farm-chemical indoor determination test rule bactericide the 6th part: the synergy of mixture measures ".
First single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on the basis of preliminary experiment, bacteriostasis rate sets by geometric progression in the scope of 5% ~ 90%).If blank, often process 4 basins, every basin 10 strain wheat seedling, repeats 4 times.By spray-on process, liquid is evenly sprayed on wheat seedling for subsequent use, naturally dry.24h after chemicals treatment, uses spore suspension spray inoculation, natural air drying, then moves to thermostatic chamber, is 20 ~ 25 DEG C in temperature, and relative moisture is the greenhouse of 70% ~ 90%.When the sick leaf rate of blank reaches more than 80%, classification investigation manages incidence everywhere, often the whole blade of process investigation.According to survey data, calculate disease index and the control efficiency of each process.By the linear regression analysis between preventive effect probability value and the logarithm value of series concentration, obtain the EC of each medicament
50value.
Calculate the co-toxicity coefficient (CTC) of mixture by the abundant method of Sun Yun, evaluate the activity of reagent agent to germ with this.The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Table 1 bacillus subtilis and the mixture synergy of prothioconazoles to wheat powdery mildew measure
2, the synergy of rice sheath blight disease mixture is measured.
In order to prevent and treat the rice sheath blight disease in agricultural production, we have carried out mutual composite synergy with bacillus subtilis and prothioconazoles and have studied, and concrete grammar is as follows.
Subjects be rice sheath blight disease (
rhizoctoniasolani).Former medicine is mixed with the test medicine of needs, test method is with reference to " farm-chemical indoor determination test rule bactericide the 4th part: suppress disease fungus mycelial growth test Plating " and " farm-chemical indoor determination test rule bactericide the 6th part: the synergy of mixture measures ".
First single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on the basis of preliminary experiment, bacteriostasis rate sets by geometric progression in the scope of 5% ~ 90%).Aseptically, according to test process, the sterilising medium melted in advance is quantitatively added in aseptic conical flask, reach high concentration from low concentration and quantitatively draw liquid successively, add respectively in above-mentioned conical flask, fully shake up.Then equivalent pours 4 diameters into is in the culture dish of 9cm, and the pastille making respective concentration is dull and stereotyped.If blank is done in the process not containing medicament, often process repetition 4 times.
By cultured pathogen aseptically, be the sterilizing card punch of 5mm, cut bacterium cake from colony edge with diameter, with inoculator, bacterium disease is inoculated in the dull and stereotyped central authorities of pastille, mycelia faces up, and covers ware lid, puts in the incubator of preference temperature and cultivates.According to the growing state investigation pathogen mycelial growth situation of bacterium in blank culture dish.Use kind of calliper colony diameter, unit is that millimeter (mm), each bacterium colony right-angled intersection method vertical survey diameter are each once, gets its mean value.Mycelial growth inhibition rate probability value according to each drug concentration logarithm value and correspondence does regression analysis, obtains the EC of each medicament
50value.
Calculate the co-toxicity coefficient (CTC) of mixture by the abundant method of Sun Yun, evaluate the activity of reagent agent to germ with this.The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Table 2 bacillus subtilis and the mixture synergy of prothioconazoles to rice sheath blight disease measure
The synergy measurement result of above two mixtures shows: bacillus subtilis and prothioconazoles are that 1:50 ~ 50:1 is mixed with weight ratio has good virulence to wheat powdery mildew and rice sheath blight disease, and especially weight ratio is that 1:30 ~ 30:1 synergistic effect is the most remarkable.
In order to better the present invention is described, be described further content of the present invention below in conjunction with embodiment, the percentage in formula is all weight percentage.
Example of formulations 1
Take 65% bacillus subtilis, 15% prothioconazoles, 5% alkyl naphthalene formaldehyde condensation product sodium sulfonate, 3% polycarboxylic acids sodium, 3% lauryl sodium sulfate, fine particle calcium carbonate fills up the weight portion to 100%.Above-mentioned raw materials is prepared into 80% bacillus subtilis prothioconazoles wetting powder through mixing, ultra micro air-flow crushing, hybrid technique step.
Example of formulations 2
Take 75% bacillus subtilis, 10% prothioconazoles, 4% calcium lignosulfonate, 5% alkyl naphthalene formaldehyde condensation product sodium sulfonate, 2% sodium alkyl sulfonate, kaolin fills up the weight portion to 100%.Above-mentioned raw materials is prepared into 85% bacillus subtilis prothioconazoles wetting powder through mixing, ultra micro air-flow crushing, hybrid technique step.
Example of formulations 3
Take 55% bacillus subtilis, 20% prothioconazoles, 5% alkylphenol-polyethenoxy base ether sulfonic acid calcium, 4% alkylnaphthalene sulfonate formaldehyde condensation products, 2% Negel, white carbon fills up the weight portion to 100%.Above-mentioned raw materials is prepared into 75% bacillus subtilis prothioconazoles wetting powder through mixing, ultra micro air-flow crushing, hybrid technique step.
Example of formulations 4
Take 60% bacillus subtilis, 10% prothioconazoles, 2% polycarboxylate TERSPERSE2700,4% sodium lignin sulfonate, 1% nekal, 3% lauryl sodium sulfate, 1% ammonium sulfate, 1% glucose, kaolin adds to 100% weight portion.Namely the method for above-mentioned raw materials frequent regulation water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step produce 70% bacillus subtilis prothioconazoles water dispersible granules.
Example of formulations 5
Take 50% bacillus subtilis, 15% prothioconazoles, 5% polycarboxylate GY-D06,3% sodium lignin sulfonate, 5% nekal, 2% neopelex, 1% polyvinylpyrrolidone, 2% starch, white carbon adds to 100% weight portion.Namely the method for above-mentioned raw materials frequent regulation water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step produce 65% bacillus subtilis prothioconazoles water dispersible granules.
Example of formulations 6
Take 50% bacillus subtilis, 10% prothioconazoles, 4% polycarboxylate T36,2% Negel formaldehyde condensation products, 3% lauryl sodium sulfate, 4% sodium naphthalene sulfonate, 2% polyvinyl alcohol, fine particle calcium carbonate adds to 100% weight portion.Namely the method for above-mentioned raw materials frequent regulation water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step produce 60% bacillus subtilis prothioconazoles water dispersible granules.
Example of formulations 7
Take 40% bacillus subtilis, 20% prothioconazoles, 3% polycarboxylic acids sodium, 3% phenethyl phenol polyethenoxy base ether phosphate, 3% sodium alkyl sulfate, 0.4% carboxymethyl cellulose, 3% polyethylene glycol, 0.2% potassium sorbate, 01% octanol, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear dispersion 30min, with sand mill sand milling to particle diameter D
90obtained 60% bacillus subtilis prothioconazoles suspending agent after being less than 10 μm.
Example of formulations 8
Take 35% bacillus subtilis, 30% prothioconazoles, 7% alkyl polyoxyethylene ether sodium sulfonate, 2% alkyl phenol formaldehyde resin polyoxyethylene ether, 0.2% carboxymethyl cellulose, 0.2% bentonite, 5% urea, 0.2% Sodium Benzoate, 1.5% silicone oil, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear dispersion 30min, with sand mill sand milling to particle diameter D
90obtained 65% bacillus subtilis prothioconazoles suspending agent after being less than 10 μm.
Example of formulations 9
Take 30% bacillus subtilis, 20% prothioconazoles, 5% sodium lignin sulfonate, 2% sodium naphthalene sulfonate, 0.3% aluminium-magnesium silicate, 0.2% methylcellulose, 5% glycerine, 0.2% formaldehyde, 1% hexanol, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear dispersion 30min, with sand mill sand milling to particle diameter D
90obtained 50% bacillus subtilis prothioconazoles suspending agent after being less than 10 μm.
Example of formulations 10
Take 20% bacillus subtilis, 15% prothioconazoles, 4% agriculture breast 2201#, 5% thermally coupled distillation columns, 5% cyclohexanone, 3% solvent naphtha S-200,1% epoxidized soybean oil, 5% sodium chloride, 0.2% polyvinyl alcohol, 0.5% benzoic acid, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear emulsification obtains 35% bacillus subtilis prothioconazoles aqueous emulsion.
Example of formulations 11
Take 25% bacillus subtilis, 20% prothioconazoles, 7% agriculture breast TX-10#, 2% Nonyl pheno (EO=10) ether phosphate, 4% triphenoethyl benzene phenol polyethenoxy ether phosphate, 2% this dish-60#, 5% dimethylbenzene, 1% triphenyl phosphite, 2% ethylene glycol, 0.2% aluminium-magnesium silicate, 0.5% formaldehyde, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear emulsification obtains 45% bacillus subtilis prothioconazoles aqueous emulsion.
Example of formulations 12
Take 30% bacillus subtilis, 25% prothioconazoles, 6% agriculture breast TX-10#, 3% agriculture breast 2201#, 4% triphenoethyl benzene phenol polyethenoxy ether phosphate, 2% this dish-60#, 5% toluene, 1% epoxychloropropane, 2% urea, 0.2% polyvinyl alcohol, 0.5% Sodium Benzoate, deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear emulsification obtains 55% bacillus subtilis prothioconazoles aqueous emulsion.
Example of formulations 13
Take 5% bacillus subtilis, 5% prothioconazoles, 3% agriculture breast 600#, 5% Negel, 3% ethyl acetate, 1% propane diols, 1% butanols, 1% polyvinyl alcohol, deionized water adds to 100% weight portion.Above-mentioned raw materials mixes through high speed shear, and ball milling 2 ~ 3 hours in ball mill, makes diameter of particle all below 5 μm, obtained 10% bacillus subtilis prothioconazoles suspension emulsion.
Example of formulations 14
Take 10% bacillus subtilis, 15% prothioconazoles, 4% agriculture breast 2201,3% polycarboxylic acids sodium, 3% repefral, 2% glycerine, 1% silicone oil, 1% bentonite, deionized water adds to 100% weight portion.Above-mentioned raw materials mixes through high speed shear, and ball milling 2 ~ 3 hours in ball mill, makes diameter of particle all below 5 μm, obtained 25% bacillus subtilis prothioconazoles suspension emulsion.
Example of formulations 15
Take 15% bacillus subtilis, 20% prothioconazoles, 5% agriculture breast 2201,4% polycarboxylic acids sodium, 3% ethyl acetate, 1% propane diols, 1% butanols, 1% white carbon, deionized water adds to 100% weight portion.Above-mentioned raw materials mixes through high speed shear, and ball milling 2 ~ 3 hours in ball mill, makes diameter of particle all below 5 μm, obtained 35% bacillus subtilis prothioconazoles suspension emulsion.
Biological Examples 1: control wheat powdery mildew field trial.
Within 2015, carry out example of formulations 3(75% bacillus subtilis prothioconazoles wetting powder in Dong Shilin village, Huai Ya town, Mei County, Baoji, Shaanxi province city), example of formulations 5(65% bacillus subtilis prothioconazoles water dispersible granules), example of formulations 9(50% bacillus subtilis prothioconazoles suspending agent), example of formulations 11(45% bacillus subtilis prothioconazoles aqueous emulsion), example of formulations 14(25% bacillus subtilis prothioconazoles suspension emulsion) prevent and treat wheat powdery mildew field trial, demonstrate the control efficiency of this medicament to wheat powdery mildew and the safety to wheat.
Trial crops is wheat, controlling object be wheat powdery mildew (
erysipegraminis).Test is located at Mei County, Baoji Huai Yazhen, and experimental field physical features is smooth, and soil is medium loam, middle fertility, pH value 7.1, and duration of test rich water quality management is medium.Test medicine and dosage refer to table 3.Separately establish blank, often process 4 times and repeat, every community 30 ㎡, Gong32Ge community, random district group arranges.Adopt conventional spray-on process, mu dispenser liquid 45kg, is evenly sprayed at leave dual sides.
Investigation and statistical method: investigate state of an illness radix before dispenser, after last dispenser, 10 ~ 14d reinvestigates once, investigate 2 times altogether.Every community diagonal fixes 5 samplings, often some investigation 0.25m
2plant, wheat is stood up shooting stage investigation base portion 1 ~ 5 leaf, first leaf under the boot leaf of the every strain of heading " Invest, Then Investigate " and boot leaf.Stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for 6% ~ 15% of whole leaf area;
5 grades: lesion area accounts for 16% ~ 25% of whole leaf area;
7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Disease index=[∑ (the sick number of sheets × relative level numerical value at different levels)/(investigation total leaf number × 9)] × 100
Control efficiency (%)=[1-(before contrast dispenser after disease index × process dispenser disease index)/(to contrast after dispenser disease index before disease index × process dispenser] × 100
Wheat powdery mildew field test results prevented and treated by table 3
Field test results shows, above 5 compounded combination have good control efficiency to wheat powdery mildew, spray 2 times by 45ga.i./mu, 94.39%, 95.44%, 95.35%, 93.14%, 92.48% is respectively to the preventive effect of 10 days after wheat powdery mildew the 2nd medicine, is obviously better than the preventive effect of each single dose.By to significance of difference analysis in 10 days after medicine, preventive effect difference reaches pole significance level (see table 3).
To the security survey of wheat, within after spray medicine after the 1st day and medicine some days, observe, each test process occurs without poisoning phenomenon wheat.
Biological Examples 2: water prevention rice sheath blight field trial.
Within 2014, carry out example of formulations 1(80% bacillus subtilis prothioconazoles wetting powder in Junichiro Koizumi village of xiangtan, hunan province county), example of formulations 6(60% bacillus subtilis prothioconazoles water dispersible granules), example of formulations 7(60% bacillus subtilis prothioconazoles suspending agent), example of formulations 12(55% bacillus subtilis prothioconazoles aqueous emulsion), example of formulations 15(35% bacillus subtilis prothioconazoles suspension emulsion) water prevention rice sheath blight field trial, demonstrate the control efficiency of this medicament to rice sheath blight disease and the safety to paddy rice.
Trial crops is paddy rice, controlling object be rice sheath blight disease (
rhizoctoniasolani).Test is located at Junichiro Koizumi village of xiangtan, hunan province county, and experimental field physical features is smooth, and soil is loam, and fertility is higher, pH value 7.2, and duration of test rich water quality management is medium.Test medicine and dosage refer to table 4.Separately establish blank, often process 4 times and repeat, every community 30 ㎡, Gong32Ge community, random district group arranges.Adopt conventional spray-on process, mu dispenser liquid 45kg, is evenly sprayed at leave dual sides.
Investigation and statistical method: investigate state of an illness radix before dispenser, after last dispenser, 10 ~ 14d reinvestigates once, investigate 2 times altogether.According to rice leaf sheath and blade damage symptom grading, in units of strain, every community diagonal 5 sampling, often some investigation is connected 5 clumps, totally 25 clumps, records total strain number, diseased plant number and sick progression.
0 grade: complete stool is anosis;
1 grade: quaterfoil and following leaf sheath, blade morbidity (taking sword-like leave as first leaf);
3 grades: Three-blade and following leaf sheath thereof, blade morbidity;
5 grades: second blade and following leaf sheath thereof, blade morbidity;
7 grades: sword-like leave blade and following leaf sheath thereof, blade morbidity;
9 grades: complete stool falls ill, withered ahead of time.
Disease index=[∑ (the sick number of sheets × relative level numerical value at different levels)/(investigation total leaf number × 9)] × 100
Control efficiency (%)=[1-(before contrast dispenser after disease index × process dispenser disease index)/(to contrast after dispenser disease index before disease index × process dispenser] × 100
table 4 water prevention rice sheath blight field test results
Field test results shows, above 5 compounded combination have good control efficiency to rice sheath blight disease, spray 2 times by 25ga.i./mu, 95.33% be respectively to the preventive effect of 10 days after rice sheath blight disease the 2nd medicine, 94.97%, 94.4.30%, 93.18%, 94.87%, be obviously better than the preventive effect of each single dose.By to significance of difference analysis in 10 days after medicine, preventive effect difference reaches pole significance level (see table 4).
To the security survey of paddy rice, within after spray medicine after the 1st day and medicine some days, observe, each test process occurs without poisoning phenomenon paddy rice.
Above two embodiment explanations, 1:50 ~ 50:1 is composite by weight for first active component bacillus subtilis and the second active component prothioconazoles, can be made into wetting powder, water dispersible granules, suspending agent, aqueous emulsion, suspension emulsion formulation, significant control efficiency is had to wheat powdery mildew, rice sheath blight disease, synergistic effect is obvious, dosage is few, control efficiency is high, and good to crop safety.
In sum, composition of the present invention is employing two kinds of active component compound schemes, its activity and bactericidal effect are not the simple superposition of each composition activity, compared with existing unitary agent, except there is significant bactericidal effect, more there is significant synergistic effect, good to doing safety, meet the security requirement of pesticidal preparations.
Claims (6)
1. one kind contains the bactericidal composition of bacillus subtilis and prothioconazoles, it is characterized in that: be made up of the first active component bacillus subtilis and the second active component prothioconazoles and auxiliary agent, the weight ratio of bacillus subtilis and prothioconazoles is 1:50 ~ 50:1.
2. contain the bactericidal composition of bacillus subtilis and prothioconazoles according to claim 1, it is characterized in that, the weight ratio of the first active component and the second active component is 1:30 ~ 30:1.
3. contain the bactericidal composition of bacillus subtilis and prothioconazoles according to claim 1, it is characterized in that, the content sum of the first active component and the second active component is 10% ~ 85% of described composition total weight.
4. contain the bactericidal composition of bacillus subtilis and prothioconazoles according to claim 3, it is characterized in that, the content sum of the first active component and the second active component is 25% ~ 80% of described composition total weight.
5. contain the bactericidal composition of bacillus subtilis and prothioconazoles according to claim 1, it is characterized in that described combination dosage form is wetting powder, water dispersible granules, suspending agent, aqueous emulsion, suspension emulsion.
6. preventing and treating the purposes in crop powdery mildew, banded sclerotial blight containing the bactericidal composition of bacillus subtilis and prothioconazoles according to claim 1.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115226718A (en) * | 2022-05-13 | 2022-10-25 | 安徽久易农业股份有限公司 | Sterilization composition containing bacillus subtilis and prothioconazole as well as preparation method and application thereof |
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Application publication date: 20151209 |