CN105112460A - Method for producing (S)-4-methylbenzene methyl propionate from cells - Google Patents
Method for producing (S)-4-methylbenzene methyl propionate from cells Download PDFInfo
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- CN105112460A CN105112460A CN201510561141.5A CN201510561141A CN105112460A CN 105112460 A CN105112460 A CN 105112460A CN 201510561141 A CN201510561141 A CN 201510561141A CN 105112460 A CN105112460 A CN 105112460A
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- candida parapsilosis
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- methyl propionate
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- 229940017219 methyl propionate Drugs 0.000 title claims description 16
- 238000004519 manufacturing process Methods 0.000 title description 3
- RJUFJBKOKNCXHH-UHFFFAOYSA-N Methyl propionate Chemical compound CCC(=O)OC RJUFJBKOKNCXHH-UHFFFAOYSA-N 0.000 title description 2
- 238000006243 chemical reaction Methods 0.000 claims abstract description 41
- 241000222173 Candida parapsilosis Species 0.000 claims abstract description 21
- 229940055022 candida parapsilosis Drugs 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 12
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 36
- 239000000243 solution Substances 0.000 claims description 19
- 230000002210 biocatalytic effect Effects 0.000 claims description 18
- 210000005253 yeast cell Anatomy 0.000 claims description 18
- 238000009423 ventilation Methods 0.000 claims description 17
- 210000004027 cell Anatomy 0.000 claims description 14
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 claims description 13
- 229960003487 xylose Drugs 0.000 claims description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 239000000758 substrate Substances 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 11
- 229940041514 candida albicans extract Drugs 0.000 claims description 11
- 239000008103 glucose Substances 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000012138 yeast extract Substances 0.000 claims description 11
- -1 3-fluorobenzoyl methyl Chemical group 0.000 claims description 8
- KXKVLQRXCPHEJC-UHFFFAOYSA-N acetic acid trimethyl ester Natural products COC(C)=O KXKVLQRXCPHEJC-UHFFFAOYSA-N 0.000 claims description 8
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 230000035484 reaction time Effects 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 208000012839 conversion disease Diseases 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000002953 phosphate buffered saline Substances 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 238000006555 catalytic reaction Methods 0.000 description 7
- 239000005515 coenzyme Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 239000001888 Peptone Substances 0.000 description 5
- 108010080698 Peptones Proteins 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 235000019319 peptone Nutrition 0.000 description 5
- 238000011218 seed culture Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000012807 shake-flask culturing Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 2
- 101710157860 Oxydoreductase Proteins 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000012847 fine chemical Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 241000024287 Areas Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000011914 asymmetric synthesis Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- YXFVVABEGXRONW-UHFFFAOYSA-N toluene Substances CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Abstract
Disclosed is a method for preparing (S)-3-(3-fluorophenyl)-methyl 3-hydroxypropanoate through candida parapsilosis cell biocatalysis. According to the method, a phosphate buffer is added into a reaction kettle, a biocatalysis reaction is completed through candida parapsilosis cells, product yield and enantiomeric excess rate are high, and difficulties of existing methods are solved.
Description
Technical field
The invention belongs to technology of pharmaceutical engineering field, be related specifically to the technology that Candida parapsilosis cell biocatalysis prepares chiral medicinal intermediate (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate.
Background technology
Chirality (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate is the important intermediate of synthesis of chiral medicine, fine chemicals, agricultural chemicals product and other exotic materialss.Biocatalysis asymmetric reaction has environmental friendliness, mild condition, selectivity advantages of higher, prepares the first approach of chipal compounds as green high-efficient, is applied to the chipal compounds producing some high added values more and more.Biocatalysis is prepared chirality (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate and is had good application prospect.
Biocatalysis has the outstanding advantages such as catalytic efficiency is high, selectivity strong, mild condition, environmental friendliness, is the important method substituting and expand traditional organic chemical synthesis in process of sustainable development.Wherein chiral separation and asymmetric synthesis are the Application Areass of biocatalysis most magnetism.As in six large fermentoids of biological catalyst, lytic enzyme catalytic kinetics resolution of racemates can obtain quiral products, in industrial biocatalytic, play key player always.In recent years, oxydo-reductase application industrially obtained and increased rapidly.At present, the ratio adopting the industry of lytic enzyme Kinetic Resolution, biological reducing method and biological oxidation process to prepare optical activity chirality compound is 4:2:1.
Biological reducing method is for Kinetic Resolution, and maximum advantage is that theoretical yield can reach 100%, and Atom economy is good.But bioreduction needs the participation of coenzyme or cofactor, limit its application to a certain extent.Due to the dependent cause of coenzyme, in bioreduction, many Bian intact cells are as catalyzer, realize the purification procedures eliminating enzyme in body while coenzyme cyclic regeneration.
(S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate is the important chiral building block of synthesis of chiral medicine, fine chemicals, agricultural chemicals product.Existing method ubiquity low conversion rate, long reaction time, needs add expensive coenzyme, high in cost of production major defect, are difficult to carry out suitability for industrialized production.The present invention will adopt biomass cells catalysis to prepare (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate.
Summary of the invention
The present invention adopts Candida parapsilosis cell catalysis to prepare (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate, and reaction formula is as follows:
Substrate 3-fluorobenzoyl methyl acetate (1), through Candida parapsilosis catalyzed reaction, obtains product (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate (2).Have multiple-microorganism can catalysis this reaction, through great many of experiments screening, finally determine adopt Candida parapsilosis as catalyzer because the effect of its catalyzed reaction is best, reaction yield, enantiomeric excess rate (ee%) are all very high.
Many Candida parapsilosis can carry out this reaction of biocatalysis, but its effect is different, differs greatly, and through experiment, the present invention selects Candida parapsilosis bacterial strain to be ATCC28473, its catalyzed reaction best results.
Because most oxydo-reductase is coenzyme NAD (P) H dependent form, and the coenzyme amount contained by cell itself may be less, therefore, when carrying out redox reaction, for promoting regenerating coenzyme, improving reaction efficiency, in reaction system, also adding cosubstrate form transformation system.Conventional cosubstrate kind is more, and the cosubstrate needed for different cell is different, and effect difference is very large, adopts several cosubstrate to combine, can obtain better effects, need carry out great many of experiments and could determine best cosubstrate.The cosubstrate that the present invention determines through great many of experiments is: wood sugar 36-39g/L, sucrose 25-28g/L, Virahol 5-6%V/V (volumetric concentration).
Because substrate solubility is lower, so, add tensio-active agent to increase the solubleness of substrate.
developing medium:
1, seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.8; The agar powder of 15g/L is added during preparation solid medium.
2, nutrient solution composition: glucose 37-44g/L, wood sugar 7-8g/L, corn plumule powder 10-12g/L, yeast extract 4-5g/L, (NH
4)
2hPO
412-15g/L, KH
2pO
46.5-7.5g/L.MgSO
4.7H
2o 0.7-0.8g/L, NaCl 0.08-0.1g/L; PH6.8.
2, solid medium composition: add the agar powder of 2% in liquid medium within.
Prepared by yeast cell.Candida parapsilosis through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 29-30 DEG C, Candida parapsilosis seed liquor is seeded to fermentor tank, inoculative proportion is 6-6.5%, and ventilation ratio is 0.7-0.9V/(V minute), cultivate 44-48 hour for 29-30 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion.Add phosphate buffered saline buffer in bottom ventilation stirred tank, pH is 7.0, adds substrate 3-fluorobenzoyl methyl acetate, makes concentration of substrate be 80-90g/L.Other compositions: malt extract 22-26g, 10 carbon polyoxyethylenated alcohol of straight chain are 21-224g/L, wood sugar 36-39g/L, sucrose 25-28g/L, Virahol 5-6%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 29-30 DEG C, add wet yeast cell and make concentration be 80-84g/L, ventilation ratio is 0.15-0.16V/(V minute), namely per minute air flow is 0.15-0.16 times of reaction solution volume, carries out biocatalytic reaction, and the reaction times is 60-64 hour; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate, reaction conversion ratio 98-99%, product yield 96-97%, enantiomeric excess rate 98.5-99%.
The present invention carries out the work and comprises bacterial strain selection, catalytic reaction condition optimization (temperature of reaction, air flow, pH), reaction medium selection and concentration optimization, comprises concentration of substrate, malt extract content, cosubstrate combination, kinds of surfactants and concentration etc.
embodiment 1
Seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.8; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: glucose 37g/L, wood sugar 78g/L, corn plumule powder 10g/L, yeast extract 4g/L, (NH
4)
2hPO
412g/L, KH
2pO
46.5g/L.MgSO
4.7H
2o 0.7g/L, NaCl 0.08g/L; PH6.8.
Wet yeast cell preparation process is as follows: Candida parapsilosis ATCC28473 through inclined-plane, shake-flask culture, obtain seed liquor.1L fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 29 DEG C, Candida parapsilosis seed liquor is seeded to fermentor tank, inoculative proportion is 6%, and ventilation ratio is 0.7V/(V minute), cultivate 44 hours for 29 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 0.5L bottom ventilation stirred reactor, pH is 7.0, add substrate 3-fluorobenzoyl methyl acetate, concentration is made to be 80g/L, malt extract 22g, 10 carbon polyoxyethylenated alcohol of straight chain are 21g/L, wood sugar 36g/L, sucrose 28g/L, Virahol 6%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 29 DEG C, add wet yeast cell and make concentration be 80g/L, ventilation ratio is 0.15V/(V minute), carry out biocatalytic reaction, the reaction times is 60 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate, reaction conversion ratio 99%, product yield 97%, enantiomeric excess rate 98.5%.
embodiment 2
Seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.8; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: glucose 44g/L, wood sugar 8g/L, corn plumule powder 12g/L, yeast extract 5g/L, (NH
4)
2hPO
415g/L, KH
2pO
47.5g/L.MgSO
4.7H
2o 0.8g/L, NaCl 0.1g/L; PH6.8.
Wet yeast cell preparation process is as follows: Candida parapsilosis ATCC28473 through inclined-plane, shake-flask culture, obtain seed liquor.5L fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 30 DEG C, Candida parapsilosis seed liquor is seeded to fermentor tank, inoculative proportion is 6.5%, and ventilation ratio is 0.8V/(V minute), cultivate 64 hours for 30 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 5L bottom ventilation stirred reactor, pH is 7.0, add substrate 3-fluorobenzoyl methyl acetate, concentration is made to be 90g/L, malt extract 22g, 10 carbon polyoxyethylenated alcohol of straight chain are 22g/L, wood sugar 9g/L, sucrose 25g/L, Virahol 5%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 30 DEG C, add wet yeast cell and make concentration be 84g/L, ventilation ratio is 0.16V/(V minute), carry out biocatalytic reaction, the reaction times is 82 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate, reaction conversion ratio 98%, product yield 96%, enantiomeric excess rate 99%.
embodiment 3
Seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.8; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: glucose 40g/L, wood sugar 7.5g/L, corn plumule powder 11g/L, yeast extract 4.5g/L, (NH
4)
2hPO
413g/L, KH
2pO
47g/L.MgSO
4.7H
2o 0.75g/L, NaCl 0.09g/L; PH6.8.
Wet yeast cell preparation process is as follows: Candida parapsilosis ATCC28473 through inclined-plane, shake-flask culture, obtain seed liquor.10L fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 30 DEG C, Candida parapsilosis seed liquor is seeded to fermentor tank, inoculative proportion is 6.3%, and ventilation ratio is 0.8V/(V minute), cultivate 48 hours for 30 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 10L bottom ventilation stirred reactor, pH is 7.0, add substrate 3-fluorobenzoyl methyl acetate, concentration is made to be 85g/L, malt extract 22g, 10 carbon polyoxyethylenated alcohol of straight chain are 22g/L, wood sugar 37g/L, sucrose 26g/L, Virahol 5.5%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 29 DEG C, add wet yeast cell and make concentration be 82g/L, ventilation ratio is 0.16V/(V minute), carry out biocatalytic reaction, the reaction times is 62 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate, reaction conversion ratio 98.5%, product yield 96.5%, enantiomeric excess rate 99%.
embodiment 4
Seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.8; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: glucose 42g/L, wood sugar 8g/L, corn plumule powder 12g/L, yeast extract 5g/L, (NH
4)
2hPO
415g/L, KH
2pO
47.5g/L.MgSO
4.7H
2o 0.75g/L, NaCl 0.1g/L; PH6.8.
Wet yeast cell preparation process is as follows: Candida parapsilosis ATCC28473 through inclined-plane, shake-flask culture, obtain seed liquor.10L fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 29 DEG C, Candida parapsilosis seed liquor is seeded to fermentor tank, inoculative proportion is 6.5%, and ventilation ratio is 0.85V/(V minute), cultivate 45 hours for 29 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 20L bottom ventilation stirred reactor, pH is 7.0, add substrate 3-fluorobenzoyl methyl acetate, concentration is made to be 86g/L, malt extract 22g, 10 carbon polyoxyethylenated alcohol of straight chain are 22g/L, wood sugar 38g/L, sucrose 27g/L, Virahol 6%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 30 DEG C, add wet yeast cell and make concentration be 83g/L, ventilation ratio is 0.15V/(V minute), carry out biocatalytic reaction, the reaction times is 63 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate, reaction conversion ratio 99%, product yield 96.5%, enantiomeric excess rate 99%.
Claims (2)
1. the method for Candida parapsilosis cell biocatalysis preparation (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate, it is characterized in that adding phosphate buffered saline buffer in retort, pH is 7.0, add substrate 3-fluorobenzoyl methyl acetate, substrate 3-fluorobenzoyl methyl acetate addition is made to be 80-90g/L, malt extract 22-26g, 10 carbon polyoxyethylenated alcohol of straight chain are 21-224g/L, wood sugar 36-39g/L, sucrose 25-28g/L, Virahol 5-6%V/V, 121 DEG C of autoclavings 30 minutes; When being cooled to 29-30 DEG C, add wet yeast cell and make concentration be 80-84g/L, ventilation ratio is 0.15-0.16V/(V minute), carry out biocatalytic reaction, the reaction times is 60-64 hour; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-(3-fluorophenyl)-3-hydroxy methyl propionate, reaction conversion ratio 98-99%, product yield 96-97%, enantiomeric excess rate 98.5-99%.
2. method according to claim 1, is characterized in that wet yeast cell preparation process is as follows: Candida parapsilosis ATCC28473, and through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 29-30 DEG C, Candida parapsilosis seed liquor is seeded to fermentor tank, inoculative proportion is 6-6.5%, and ventilation ratio is 0.7-0.9V/(V minute), cultivate 44-48 hour for 29-30 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal; In fermentor tank, nutrient solution composition is: glucose 37-44g/L, wood sugar 7-8g/L, corn plumule powder 10-12g/L, yeast extract 4-5g/L, (NH
4)
2hPO
412-15g/L, KH
2pO
46.5-7.5g/L.MgSO
4.7H
2o 0.7-0.8g/L, NaCl 0.08-0.1g/L; PH6.8.
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|---|---|---|---|
| CN201510561141.5A CN105112460A (en) | 2015-09-07 | 2015-09-07 | Method for producing (S)-4-methylbenzene methyl propionate from cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510561141.5A CN105112460A (en) | 2015-09-07 | 2015-09-07 | Method for producing (S)-4-methylbenzene methyl propionate from cells |
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|---|---|
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ID=54660568
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|---|---|---|---|
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Application publication date: 20151202 |
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