CN105087705A - Method for producing (S)-3-benzofuranol through saccharomyces cerevisiae - Google Patents
Method for producing (S)-3-benzofuranol through saccharomyces cerevisiae Download PDFInfo
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- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 20
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 title abstract description 4
- 238000004519 manufacturing process Methods 0.000 title abstract description 4
- 238000006243 chemical reaction Methods 0.000 claims abstract description 41
- 238000000034 method Methods 0.000 claims abstract description 10
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 36
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 21
- 210000005253 yeast cell Anatomy 0.000 claims description 21
- 239000000243 solution Substances 0.000 claims description 19
- 230000002210 biocatalytic effect Effects 0.000 claims description 18
- 238000009423 ventilation Methods 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 11
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 claims description 11
- 229940041514 candida albicans extract Drugs 0.000 claims description 11
- 239000008103 glucose Substances 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 11
- 229960003487 xylose Drugs 0.000 claims description 11
- 239000012138 yeast extract Substances 0.000 claims description 11
- 210000004027 cell Anatomy 0.000 claims description 10
- MGKPCLNUSDGXGT-UHFFFAOYSA-N 1-benzofuran-3-one Chemical compound C1=CC=C2C(=O)COC2=C1 MGKPCLNUSDGXGT-UHFFFAOYSA-N 0.000 claims description 8
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 7
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 7
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 7
- 239000001888 Peptone Substances 0.000 claims description 7
- 108010080698 Peptones Proteins 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 235000019319 peptone Nutrition 0.000 claims description 7
- 230000035484 reaction time Effects 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- 229910052799 carbon Inorganic materials 0.000 claims description 6
- 208000012839 conversion disease Diseases 0.000 claims description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000002953 phosphate buffered saline Substances 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- 241000790917 Dioxys <bee> Species 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 238000006555 catalytic reaction Methods 0.000 abstract description 9
- 238000007796 conventional method Methods 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 11
- 239000000758 substrate Substances 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 229920001817 Agar Polymers 0.000 description 7
- 239000008272 agar Substances 0.000 description 7
- 239000005515 coenzyme Substances 0.000 description 7
- 239000007787 solid Substances 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 5
- 238000011218 seed culture Methods 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000012807 shake-flask culturing Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 2
- 101710157860 Oxydoreductase Proteins 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
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- 239000012847 fine chemical Substances 0.000 description 2
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- 239000013028 medium composition Substances 0.000 description 2
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- 102000004190 Enzymes Human genes 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
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- 230000001580 bacterial effect Effects 0.000 description 1
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- 125000004122 cyclic group Chemical group 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing (S)-3-benzofuranol through biological catalysis by adopting saccharomyces cerevisiae cells. A phosphate buffer solution is added into a reaction tank, and the biological catalysis reaction is carried out by adopting the saccharomyces cerevisiae cells. The product yield is high, the enantiomeric excess rate is high, and the difficulty of production adopting the conventional method is solved.
Description
Technical field
The invention belongs to technology of pharmaceutical engineering field, be related specifically to the technology that brewing yeast cell biocatalysis prepares chiral medicinal intermediate (S)-3-cumarone alcohol.
Background technology
Chirality (S)-3-cumarone alcohol is the important intermediate of synthesis of chiral medicine, fine chemicals, agricultural chemicals product and other exotic materialss.Biocatalysis asymmetric reaction has environmental friendliness, mild condition, selectivity advantages of higher, prepares the first approach of chipal compounds as green high-efficient, is applied to the chipal compounds producing some high added values more and more.Biocatalysis is prepared chirality (S)-3-cumarone alcohol and is had good application prospect.
Biocatalysis has the outstanding advantages such as catalytic efficiency is high, selectivity strong, mild condition, environmental friendliness, is the important method substituting and expand traditional organic chemical synthesis in process of sustainable development.Wherein chiral separation and asymmetric synthesis are the Application Areass of biocatalysis most magnetism.As in six large fermentoids of biological catalyst, lytic enzyme catalytic kinetics resolution of racemates can obtain quiral products, in industrial biocatalytic, play key player always.In recent years, oxydo-reductase application industrially obtained and increased rapidly.At present, the ratio adopting the industry of lytic enzyme Kinetic Resolution, biological reducing method and biological oxidation process to prepare optical activity chirality compound is 4:2:1.
Biological reducing method is for Kinetic Resolution, and maximum advantage is that theoretical yield can reach 100%, and Atom economy is good.But bioreduction needs the participation of coenzyme or cofactor, limit its application to a certain extent.Due to the dependent cause of coenzyme, in bioreduction, many Bian intact cells are as catalyzer, realize the purification procedures eliminating enzyme in body while coenzyme cyclic regeneration.
(S)-3-cumarone alcohol is the important chiral building block of synthesis of chiral medicine, fine chemicals, agricultural chemicals product.Existing method ubiquity low conversion rate, long reaction time, needs add expensive coenzyme, high in cost of production major defect, are difficult to carry out suitability for industrialized production.The present invention will adopt biomass cells catalysis to prepare (S)-3-cumarone alcohol.
Summary of the invention
The present invention adopts brewing yeast cell catalysis to prepare (S)-3-cumarone alcohol, and reaction formula is as follows:
3-benzofuranone (1), through yeast saccharomyces cerevisiae catalyzed reaction, obtains product (S)-3-cumarone alcohol (2).Have multiple-microorganism can catalysis this reaction, through great many of experiments screening, finally determine adopt yeast saccharomyces cerevisiae as catalyzer because the effect of its catalyzed reaction is best, reaction yield, enantiomeric excess rate (ee%) are all very high.
Many yeast saccharomyces cerevisiaes can carry out this reaction of biocatalysis, but its effect is different, differs greatly, and through experiment, the present invention selects Wine brewing yeast strain to be ATCC3666, its catalyzed reaction best results.
Because most oxydo-reductase is coenzyme NAD (P) H dependent form, and the coenzyme amount contained by cell itself may be less, therefore, when carrying out redox reaction, for promoting regenerating coenzyme, improving reaction efficiency, in reaction system, also adding cosubstrate form transformation system.Conventional cosubstrate kind is more, and the cosubstrate needed for different cell is different, and effect difference is very large, adopts several cosubstrate to combine, can obtain better effects, need carry out great many of experiments and could determine best cosubstrate.The cosubstrate that the present invention determines through great many of experiments is: wood sugar 20-24g/L, sucrose 25-28g/L, maltose 36-40g/L, Virahol 8-10%V/V (volumetric concentration).
Because substrate solubility is lower, so, add tensio-active agent to increase the solubleness of substrate.
developing medium:
1, seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.4; The agar powder of 15g/L is added during preparation solid medium.
2, nutrient solution composition: peptone 1.5-2g/L, yeast extract 8-10g/L, glucose 18-22g/L, malt extract 25-30g, ammonium sulfate 0.2-0.25g/L, magnesium sulfate 0.3-0.4g/L, phosphoric acid dioxy potassium 1.8-2.3g/L; Solid medium composition: add the agar powder of 2% in liquid medium within; PH6.4.
2, solid medium composition: add the agar powder of 2% in liquid medium within.
Prepared by yeast cell.Yeast saccharomyces cerevisiae through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 28-29 DEG C, yeast saccharomyces cerevisiae seed liquor is seeded to fermentor tank, inoculative proportion is 6-6.5%, and ventilation ratio is 1.1-1.3V/(V minute), cultivate 44-48 hour for 28-29 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion.Add phosphate buffered saline buffer in bottom ventilation stirred tank, pH is 6.0, adds substrate 3-benzofuranone, makes concentration of substrate be 80-90g/L.Other compositions: malt extract 22-26g, collateralization 13 carbon Guerbet polyoxyethylenated alcohol 17-18g/L, wood sugar 20-24g/L, sucrose 25-28g/L, maltose 36-40g/L, Virahol 8-10%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 28-29 DEG C, add wet yeast cell and make concentration be 75-79g/L, ventilation ratio is 0.15-0.16V/(V minute), namely per minute air flow is 0.15-0.16 times of reaction solution volume, carries out biocatalytic reaction, and the reaction times is 77-81 hour; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-cumarone alcohol, reaction conversion ratio 98-99%, product yield 96-97%, enantiomeric excess rate 98.5-99%.
The present invention carries out the work and comprises bacterial strain selection, catalytic reaction condition optimization (temperature of reaction, air flow, pH), reaction medium selection and concentration optimization, comprises concentration of substrate, malt extract content, cosubstrate combination, kinds of surfactants and concentration etc.
embodiment 1
Seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.4; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: glucose 37g/L, wood sugar 78g/L, corn plumule powder 10g/L, yeast extract 4g/L, (NH
4)
2hPO
412g/L, KH
2pO
46.5g/L.MgSO
4.7H
2o 0.7g/L, NaCl 0.08g/L; PH6.4.
Wet yeast cell preparation process is as follows: yeast saccharomyces cerevisiae ATCC3666 through inclined-plane, shake-flask culture, obtain seed liquor.1L fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 28 DEG C, yeast saccharomyces cerevisiae seed liquor is seeded to fermentor tank, inoculative proportion is 6%, and ventilation ratio is 1.1V/(V minute), cultivate 44 hours for 28 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 0.5L bottom ventilation stirred reactor, pH is 6.0, add substrate 3-benzofuranone, concentration is made to be 80g/L, malt extract 22g, collateralization 13 carbon Guerbet polyoxyethylenated alcohol 17g/L, wood sugar 24g/L, sucrose 25g/L, maltose 40g/L, Virahol 10%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 28 DEG C, add wet yeast cell and make concentration be 75g/L, ventilation ratio is 0.15V/(V minute), carry out biocatalytic reaction, the reaction times is 75 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-cumarone alcohol, reaction conversion ratio 99%, product yield 97%, enantiomeric excess rate 98.5%.
embodiment 2
Seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.4; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: glucose 44g/L, wood sugar 8g/L, corn plumule powder 12g/L, yeast extract 5g/L, (NH
4)
2hPO
415g/L, KH
2pO
47.5g/L.MgSO
4.7H
2o 0.8g/L, NaCl 0.1g/L; PH6.4.
Wet yeast cell preparation process is as follows: yeast saccharomyces cerevisiae ATCC3666 through inclined-plane, shake-flask culture, obtain seed liquor.5L fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 29 DEG C, yeast saccharomyces cerevisiae seed liquor is seeded to fermentor tank, inoculative proportion is 6.5%, and ventilation ratio is 1.3V/(V minute), cultivate 75 hours for 29 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 5L bottom ventilation stirred reactor, pH is 6.0, add substrate 3-benzofuranone, concentration is made to be 90g/L, malt extract 22g, collateralization 13 carbon Guerbet polyoxyethylenated alcohol 18g/L, wood sugar 20g/L, sucrose 28g/L, maltose 36g/L, Virahol 8%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 29 DEG C, add wet yeast cell and make concentration be 79g/L, ventilation ratio is 0.16V/(V minute), carry out biocatalytic reaction, the reaction times is 79 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-cumarone alcohol, reaction conversion ratio 98%, product yield 96%, enantiomeric excess rate 99%.
embodiment 3
Seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.4; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: glucose 40g/L, wood sugar 7.5g/L, corn plumule powder 11g/L, yeast extract 4.5g/L, (NH
4)
2hPO
413g/L, KH
2pO
47g/L.MgSO
4.7H
2o 0.75g/L, NaCl 0.09g/L; PH6.4.
Wet yeast cell preparation process is as follows: yeast saccharomyces cerevisiae ATCC3666 through inclined-plane, shake-flask culture, obtain seed liquor.10L fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 28 DEG C, yeast saccharomyces cerevisiae seed liquor is seeded to fermentor tank, inoculative proportion is 6.3%, and ventilation ratio is 1.2V/(V minute), cultivate 48 hours for 28 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 10L bottom ventilation stirred reactor, pH is 6.0, add substrate 3-benzofuranone, concentration is made to be 92g/L, malt extract 22g, collateralization 13 carbon Guerbet polyoxyethylenated alcohol 17g/L, wood sugar 22g/L, sucrose 26g/L, maltose 38g/L, Virahol 9.5%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 28 DEG C, add wet yeast cell and make concentration be 77g/L, ventilation ratio is 0.16V/(V minute), carry out biocatalytic reaction, the reaction times is 77 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-cumarone alcohol, reaction conversion ratio 98.5%, product yield 96.5%, enantiomeric excess rate 99%.
embodiment 4
Seed culture medium composition is: yeast extract 10g/L, glucose 20g/L, peptone 20g/L, pH6.4; The agar powder of 15g/L is added during preparation solid medium.
Nutrient solution composition is: glucose 42g/L, wood sugar 8g/L, corn plumule powder 12g/L, yeast extract 5g/L, (NH
4)
2hPO
415g/L, KH
2pO
47.5g/L.MgSO
4.7H
2o 0.75g/L, NaCl 0.1g/L; PH6.4.
Wet yeast cell preparation process is as follows: yeast saccharomyces cerevisiae ATCC3666 through inclined-plane, shake-flask culture, obtain seed liquor.10L fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 29 DEG C, yeast saccharomyces cerevisiae seed liquor is seeded to fermentor tank, inoculative proportion is 6.5%, and ventilation ratio is 1.18V/(V minute), cultivate 45 hours for 29 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.
Biocatalytic Conversion: add phosphate buffered saline buffer in 20L bottom ventilation stirred reactor, pH is 6.0, add substrate 3-benzofuranone, concentration is made to be 84g/L, malt extract 22g, collateralization 13 carbon Guerbet polyoxyethylenated alcohol 18g/L, wood sugar 23g/L, sucrose 27g/L, maltose 39g/L, Virahol 9%V/V (volumetric concentration), 121 DEG C of autoclavings 30 minutes; When being cooled to 29 DEG C, add wet yeast cell and make concentration be 78g/L, ventilation ratio is 0.15V/(V minute), carry out biocatalytic reaction, the reaction times is 78 hours; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-cumarone alcohol, reaction conversion ratio 99%, product yield 96.5%, enantiomeric excess rate 99%.
Claims (2)
1. the method for brewing yeast cell biocatalysis preparation (S)-3-cumarone alcohol, it is characterized in that adding phosphate buffered saline buffer in retort, pH is 6.0, add 3-benzofuranone, 3-benzofuranone addition is made to be 80-90g/L, malt extract 22-26g, collateralization 13 carbon Guerbet polyoxyethylenated alcohol 17-18g/L, wood sugar 20-24g/L, sucrose 25-28g/L, maltose 36-40g/L, Virahol 8-10%V/V, 121 DEG C of autoclavings 30 minutes; When being cooled to 28-29 DEG C, add wet yeast cell and make concentration be 75-79g/L, ventilation ratio is 0.15-0.16V/(V minute), carry out biocatalytic reaction, the reaction times is 77-81 hour; After reaction terminates, leach cell, be extracted with ethyl acetate reaction solution, steam ethyl acetate, obtain product (S)-3-cumarone alcohol, reaction conversion ratio 98-99%, product yield 96-97%, enantiomeric excess rate 98.5-99%.
2. method according to claim 1, is characterized in that wet yeast cell preparation process is as follows: yeast saccharomyces cerevisiae ATCC3666, and through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, 121 DEG C of autoclavings 30 minutes, be cooled to 28-29 DEG C, yeast saccharomyces cerevisiae seed liquor is seeded to fermentor tank, inoculative proportion is 6-6.5%, and ventilation ratio is 1.1-1.3V/(V minute), cultivate 44-48 hour for 28-29 DEG C, wet yeast cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal; In fermentor tank, nutrient solution composition is: peptone 1.5-2g/L, yeast extract 8-10g/L, glucose 18-22g/L, malt extract 25-30g, ammonium sulfate 0.2-0.25g/L, magnesium sulfate 0.3-0.4g/L, phosphoric acid dioxy potassium 1.8-2.3g/L; PH6.4.
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