CN105092680B - Cartridge device applied to HPCE - Google Patents
Cartridge device applied to HPCE Download PDFInfo
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- CN105092680B CN105092680B CN201510506948.9A CN201510506948A CN105092680B CN 105092680 B CN105092680 B CN 105092680B CN 201510506948 A CN201510506948 A CN 201510506948A CN 105092680 B CN105092680 B CN 105092680B
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- capillary
- gel
- cartridge device
- hpce
- syringe needle
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Abstract
The invention discloses a kind of cartridge device applied to HPCE, and it includes body, and the gel memotron in body；Capillary mounting hole and the capillary cavity being connected with capillary mounting hole are provided with body, syringe needle installation portion is provided with body, sampling syringe needle is installed in syringe needle installation portion, the sampling syringe needle is stretched out outside body, capillary is installed in capillary mounting hole, one end of capillary is stretched into sampling syringe needle, and the other end is stretched into gel memotron, is wound in the middle part of capillary in capillary cavity.By setting capillary cavity, capillary is wound in capillary cavity the present invention, cartridge device is installed the capillary of different length, to adapt to the analysis of different samples detection.
Description
Technical field
The present invention relates to HPCE field, and in particular to a kind of cartridge device applied to HPCE.
Background technology
Bioanalysis, such as DNA analysis, carrying out exploring to bigger and from pursuing accurate pure science
The fast transition of the conventional program of the reliability of confirmation.Capillary Electrophoresis (capillary electrophoresis, CE) is one
Novel liquid-phase isolation technics of the class using capillary as split tunnel, using high-voltage dc as driving force, generally use capillary
Electrophoresis apparatus carries out DNA detection and analysis and Separation of Proteins analysis.But the cartridge device of existing HPCE includes body,
The capillary mounting hole of installation capillary is offered in the body, the length of the capillary depends on the length of body, therefore needs
When changing detection sample, it may be necessary to be moved to another HPCE and detected, it is low that this has resulted in operating efficiency
Down, it is necessary to put into more HPCEs, input cost is caused to improve.And the separation of existing HPCE is led to
Road, detection window, electrode and gel storage it is canned be separate part, cause apparatus structure complicated, manufacturing cost is high,
It is unfavorable for daily maintenance, also make it that changing capillary and the operating personnel of gel needs higher technical capability.
The content of the invention
(1) technical problems to be solved
The technical problem to be solved in the present invention is to provide a kind of simple in construction, different length capillary is can configure, is applied to
The cartridge device of different Capillary Electrophoresis.
(2) technical scheme
In order to solve the above technical problems, the present invention provides a kind of cartridge device applied to HPCE, it includes
Body, and the gel memotron in body；Be provided with body capillary mounting hole and with capillary mounting hole
The capillary cavity being connected, syringe needle installation portion is provided with body, sampling syringe needle is installed, the sampling syringe needle in syringe needle installation portion
Stretch out outside body, capillary is installed in capillary mounting hole, and one end of capillary is stretched into sampling syringe needle, and the other end stretches into solidifying
In glue memotron, it is wound in the middle part of capillary in capillary cavity.
Wherein, detection window is offered at capillary mounting hole in body.
Wherein, the both ends in body positioned at detection window are respectively arranged with shading sleeve, and capillary sequentially passes through shading sleeve.
Wherein, annular groove is offered on gel memotron, gel memotron is buckled in body by annular groove.
Wherein, body after upper and lower two and half bodies pairing by being formed by connecting.
Wherein, the edge of at least one half body has opening.
Wherein, multiple reinforcements are evenly arranged with each half body.
Wherein, fluting is offered on reinforcement.
Wherein, cartridge device also includes metal electrode P (i.e. positive pole, P are English positive abbreviation) and metal electrode N
(i.e. negative pole, P are English negative abbreviation), metal electrode P is installed in the first mounting hole of gel memotron, metal electricity
Pole N is set in the second mounting hole of syringe needle installation portion.
Wherein, the first mounting hole is connected with storing the cavity of gel on gel memotron.
(3) beneficial effect
Compared with prior art, the present invention has following features：
(1) by setting capillary cavity, capillary is wound in capillary cavity, cartridge device is installed different length
Capillary, to adapt to the analysis of different samples detection；
(2) four capillary in capillary electrophoresis, detection window, electrode and gel storage key components are integrated
For an entirety, apparatus structure is simplified, reduces fault rate, be easy to daily maintenance, the operation of personnel easy to operation；
(3) present invention turns into a kind of special consumptive material, can matched somebody with somebody according to different application purposes independently of instrument host part
The capillary of the gels of standby different formulations or specific standard (such as the bore of capillary, length, coating etc.), and replacing side
Just；
(4) Optical devices are not contained in the present invention, its split tunnel with detection area without carrying out optics fine alignment.
Brief description of the drawings
Fig. 1 is the dimensional structure diagram for the cartridge device that the present invention is applied to HPCE.
Fig. 2 is that the present invention removes the dimensional structure diagram after half body applied to the cartridge device of HPCE.
Fig. 3 is the dimensional structure diagram of half body of the cartridge device that the present invention is applied to HPCE.
In figure, 1- bodies；2- gel memotrons；3- sampling syringe needles；4- capillaries；5- shading sleeves；11- capillaries are installed
Hole；12- capillary cavities；13- detection windows；14- slots；15- is open；16- reinforcements；17- mounting grooves；18- syringe needle installation portions；
The mounting holes of 19- second；21- annular grooves；22- lids；23- bodys；The mounting holes of 24- first；51- steps.
Embodiment
With reference to the accompanying drawings and examples, the embodiment of the present invention is described in further detail.Implement below
Example is used to illustrate the present invention, but can not be used for limiting the scope of the present invention.
The present invention is applied to the stereochemical structure of the cartridge device of HPCE as depicted in figs. 1 and 2, the cartridge device
Including body 1, installed in the gel memotron 2 of body 1, capillary mounting hole 11 and capillary installation are provided with body 1
The capillary cavity 12 that hole 11 is connected, the syringe needle installation portion 18 of installation sampling syringe needle 3 is provided with body 1, and the sampling syringe needle 3 is stretched
Go out outside above-mentioned body 1, capillary 4 is installed in above-mentioned capillary mounting hole 11, and above-mentioned sampling pin is stretched into one end of the capillary 4
In first 3, the other end is stretched into above-mentioned gel memotron 2, and the middle part of the capillary 4 is wound in capillary cavity 12.
Detection window 13 is offered in above-mentioned body 1, the detection window 13 is right against capillary mounting hole 11, makes capillary
It is exposed, laser excitation device and light signal receiving are installed outside detection window 13, the optical fiber that the laser excitation device is sent
The flow direction of direction and capillary is in 45 °, and light signal receiving direction is also in 45 ° with the flow direction with capillary, can avoid optical fiber
It is emitted directly toward on light signal receiving, avoids the influence of too strong light.
Both ends in body 1 positioned at detection window 13 are respectively arranged with shading sleeve 5, and capillary 4 sequentially passes through shading sleeve 5,
The size of adjustable detection window.
Mounting groove 17 is offered in above-mentioned body 1, the step 51 of above-mentioned shading sleeve 5 is buckled in mounting groove 17, ensures to hide
Light set is fixedly mounted in body.
Annular groove 21 is offered on gel memotron 2, gel memotron 2 is buckled in body 1 by annular groove 21, is simplified
The connected mode of gel memotron and body, and it is simple in construction, easy for installation.
Above-mentioned gel memotron 2 includes body 23 and the lid 22 on body 23, and installation is offered on body 23
The cavity of gel, above-mentioned annular groove are opened on body 23, are easy to the installation of gel memotron, the setting of detachable lid is just
Load in cavity or change the gel in cavity in gel.
Above-mentioned capillary is fixedly mounted in gel memotron by fixture (not shown), and be immersed in gel it
In, and seal is provided between capillary and fixture, avoid gel from leaking.
Body 1 after upper and lower two and half bodies pairing by being formed by connecting, and the structure of half body is as shown in figure 3, so can be so as to
In the installation of gel memotron and capillary.
The edge of at least one half body has opening 15, and opening can be opened in one and half bodies, can also be
All open up opening in two and half bodies, during dismounting, be easy to hand or other objects to stretch into and lift up out lower half body, be easy to capillary
Replacing.
Multiple reinforcements 16 are evenly arranged with each half body, improve the structural strength of body；On the reinforcement 16
Fluting 14 is offered, for positioning capillary.
Above-mentioned cartridge device also includes metal electrode P and metal electrode N, and metal electrode P is installed on the of gel memotron 2
In one mounting hole 24, the first mounting hole 24 is connected with storing the cavity of gel on gel memotron 2, and the one of metal electrode P
Outside, the other end directly contacts with the gel in cavity at end；Metal electrode N is set in the second installation of syringe needle installation portion 18
In hole 19, metal electrode N is enclosed in the second mounting hole shaped like a draw point, can not only protect capillary but also can realize electrode
Effect.
During work, first apply certain pressure in gel memotron, gel is flowed into capillary, then sampling syringe needle is put
It is placed in sample, then is positioned in buffer solution, and apply voltage at both ends, now sample electrophoresis in capillary, in gel
Under effect, sample molecules produce separation, sample molecules generation fluorescence reaction under the exciting of laser, and are gathered simultaneously by reception device
It is analyzed.By setting capillary cavity, capillary is wound in capillary cavity the present invention, enables same middle cartridge device
The capillary of a variety of different lengths is installed, to adapt to the detection of different samples, different capillarys need to be only changed when detecting different samples
The cartridge device of length of tube；And by the capillary in capillary electrophoresis, detection window, electrode and gel storage four
Individual key component is integrated into an entirety, simplifies apparatus structure, reduces fault rate, and is allowed to independently of instrument host portion
Point, turn into a kind of special consumptive material, be easy to daily maintenance, the operation of personnel easy to operation.
It these are only the preferred embodiment of the present invention, it is noted that come for those skilled in the art
Say, without departing from the technical principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (8)
- A kind of 1. cartridge device applied to HPCE, it is characterised in that：The cartridge device includes body (1), with And the gel memotron (2) in the body (1)；Be provided with the body (1) capillary mounting hole (11) and The capillary cavity (12) being connected with the capillary mounting hole (11), syringe needle installation portion (18) is provided with the body (1), Sampling syringe needle (3) is installed, the sampling syringe needle (3) stretches out the body (1) outside, capillary in the syringe needle installation portion (18) (4) it is installed in the capillary mounting hole (11), one end of the capillary (4) is stretched into the sampling syringe needle (3), another End is stretched into the gel memotron (2), is wound in the middle part of the capillary (4) in the capillary cavity (12), the casket On body (1) detection window (13) is offered positioned at capillary mounting hole (11) place；The cartridge device also includes metal electrode P and metal electrode N, the metal electrode P are installed on the gel memotron (2) in the first mounting hole (24), the metal electrode N is set in the second mounting hole (19) of the syringe needle installation portion (18) It is interior.
- 2. the cartridge device according to claim 1 applied to HPCE, it is characterised in that：The body (1) The interior both ends positioned at the detection window (13) are respectively arranged with shading sleeve (5), and the capillary (4) sequentially passes through the shading Cover (5).
- 3. the cartridge device according to claim 1 applied to HPCE, it is characterised in that：The gel storage Annular groove (21) is offered on pipe (2), the gel memotron (2) is buckled in the body (1) by the annular groove (21) On.
- 4. the cartridge device applied to HPCE according to any one of claims 1 to 3, it is characterised in that：Institute Body (1) is stated by being formed by connecting after upper and lower two and half bodies pairing.
- 5. the cartridge device according to claim 4 applied to HPCE, it is characterised in that：It is at least one described The edge of half body has opening (15).
- 6. the cartridge device according to claim 4 applied to HPCE, it is characterised in that：Each half casket All it is evenly arranged with multiple reinforcements (16) in vivo.
- 7. the cartridge device according to claim 6 applied to HPCE, it is characterised in that：The reinforcement (16) fluting (14) is offered on.
- 8. the cartridge device according to claim 1 applied to HPCE, it is characterised in that：First installation Hole (24) is connected with storing the cavity of gel on the gel memotron (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510506948.9A CN105092680B (en) | 2015-08-18 | 2015-08-18 | Cartridge device applied to HPCE |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510506948.9A CN105092680B (en) | 2015-08-18 | 2015-08-18 | Cartridge device applied to HPCE |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105092680A CN105092680A (en) | 2015-11-25 |
| CN105092680B true CN105092680B (en) | 2018-03-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510506948.9A Active CN105092680B (en) | 2015-08-18 | 2015-08-18 | Cartridge device applied to HPCE |
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| Country | Link |
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| CN (1) | CN105092680B (en) |
Citations (10)
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| US4911807A (en) * | 1989-09-05 | 1990-03-27 | Bio-Rad Laboratories, Inc. | Fractionation and sample loading by cassette in capillary electrophoresis |
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| US6870165B2 (en) * | 2001-10-19 | 2005-03-22 | Biocal Technology, Inc. | Multi-color multiplexed analysis in a bio-separation system |
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| CN103868970A (en) * | 2014-03-21 | 2014-06-18 | 上海通微分析技术有限公司 | Full-automatic high-precision capillary electrophoresis apparatus |
| CN203732553U (en) * | 2013-12-30 | 2014-07-23 | 深圳市理邦精密仪器股份有限公司 | Medical capillary adapter, test card component and blood gas analyzer |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0984276A4 (en) * | 1998-02-16 | 2003-08-27 | Inst Physical & Chem Res | Capillary cassette and method of producing the same |
| US20060194306A1 (en) * | 2003-08-25 | 2006-08-31 | Herr Amy E | System for gel electrophoretic immunoassay |
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2015
- 2015-08-18 CN CN201510506948.9A patent/CN105092680B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002139475A (en) * | 1985-09-18 | 2002-05-17 | Univ Leland Stanford Jr | Fluorescence detection and interface electrokinetic separation method and device |
| US4911807A (en) * | 1989-09-05 | 1990-03-27 | Bio-Rad Laboratories, Inc. | Fractionation and sample loading by cassette in capillary electrophoresis |
| DE4230355A1 (en) * | 1991-09-10 | 1993-03-11 | Hitachi Ltd | DNA measuring process and appts. - using fluorescent marker at end of mol. which is straightened and passed through zone to detect interval between markers |
| CN1494654A (en) * | 2001-01-26 | 2004-05-05 | ¿ | Multi-channel bio-separation cartridge |
| US6870165B2 (en) * | 2001-10-19 | 2005-03-22 | Biocal Technology, Inc. | Multi-color multiplexed analysis in a bio-separation system |
| CN1485614A (en) * | 2002-09-24 | 2004-03-31 | 财团法人工业技术研究院 | Card box for electrophoresis checking apparatus and method for manufacturing the same |
| CN101165472A (en) * | 2006-10-20 | 2008-04-23 | 中国科学院大连化学物理研究所 | Multiple-pass mode capillary tube electrophoresis device |
| CN103328963A (en) * | 2010-08-24 | 2013-09-25 | 光鼎生物科技股份有限公司 | Disposible bio-analysis cartridge and instrument for conducting bio-analysis using same |
| CN203732553U (en) * | 2013-12-30 | 2014-07-23 | 深圳市理邦精密仪器股份有限公司 | Medical capillary adapter, test card component and blood gas analyzer |
| CN103868970A (en) * | 2014-03-21 | 2014-06-18 | 上海通微分析技术有限公司 | Full-automatic high-precision capillary electrophoresis apparatus |
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| Publication number | Publication date |
|---|---|
| CN105092680A (en) | 2015-11-25 |
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Address after: No.396, Mingzhu Road, science and Technology Park, Ningbo City, Zhejiang Province Patentee after: Ningbo Haier Shi Gene Technology Co.,Ltd. Address before: No.396, Mingzhu Road, science and Technology Park, Ningbo City, Zhejiang Province Patentee before: Ningbo Health Gene Technologies Co.,Ltd. |
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