CN105079863A - Preparation method of aloe/sodium alginate double-layer hydrogel dressing - Google Patents
Preparation method of aloe/sodium alginate double-layer hydrogel dressing Download PDFInfo
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- CN105079863A CN105079863A CN201510490702.7A CN201510490702A CN105079863A CN 105079863 A CN105079863 A CN 105079863A CN 201510490702 A CN201510490702 A CN 201510490702A CN 105079863 A CN105079863 A CN 105079863A
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Abstract
The invention discloses a preparation method of aloe/sodium alginate double-layer hydrogel dressing. The method includes: adding plasticizer glycerol into sodium alginate aqueous solution, stirring, performing ultrasonic treatment, standing at room temperature to obtain sodium alginate gel, dripping the sodium alginate gel into a culture dish, and drying to obtain a sodium alginate substrate layer; adding aloe aqueous solution into the sodium alginate aqueous solution, stirring, performing ultrasonic treatment, and standing at room temperature to obtain aloe/sodium alginate mixed gel; dripping the aloe/sodium alginate mixed gel on the surface of the sodium alginate substrate layer, and drying; soaking the double-layer hydrogel film into CaCl2 solution for 5-20 minutes, then washing with distilled water, and drying to obtain the aloe/sodium alginate double-layer hydrogel dressing. The method has the advantages that the hydrogel dressing with high transparency and swelling rate is prepared through the simple method, and the prepared dressing has excellent antibacterial performance, can promote fibroblast proliferation ability, and has weak sensitization and no irritation to the skin.
Description
Technical field
The present invention relates to the preparation method of a kind of Aloe/sodium alginate Double-layer water gel dressing.
Background technology
At present, absorbent cotton, gauze, binder are the dry dressings that hospital's process wound is conventional, serve certain effect, but still there is many deficiencies: as dry dressing poor air permeability, easily cause exogenous infection when soaking in wound immediate union; Wound surface wettability can not be kept, postpone the healing of wound surface; Can foreign body reaction be caused, have a strong impact on wound healing; Dressing easily with wound granulation tissue adhesion, cause pain of patients changing dressing and damage cambium.In order to overcome above-mentioned shortcoming, researcheres are according to " moist wounds healing theoretical " of doctor Winter (under airtight moist environment speed of wound healing ratio be exposed to air drying wound surface fast again) (WinterGD.
nature, 1962,193,293-294) and begin one's study multiple moist dressing.Wherein, the hydrogel material of high retentiveness have can wound circumference create and maintain a moistening environment thus accelerating wound (BoatengJS, MatthewsKH, StevensHNE,
etal.
journalofPharmaceuticalSciences, 2008,97,2892-2923) etc. advantage and receiving much concern.This is because hydrogel is that a class is enclosed with the hydrophilic of large quantity of moisture or the polymeric material of amphiphilic macromolecular three-dimensional net structure, a large amount of water and physiological solutions can be absorbed, and the gel-like substance (TsurutaT.BiomedicalApplicationsofPolymericMaterial in indissolubility in water
cRC, 1993), possess the features such as high-hydrophilic, high viscoelasticity and good biocompatibility, make it have application prospect at biomedicine field.
On the other hand, along with the enhancing of environmental consciousness, present multiselect hypotoxicity, degradable natural macromolecular material carry out synthetic water gel as raw material.Sodium alginate (SodiumAlginate, SA) is the anion polysaccharide that one derives from pure natural plant-brown algae, has solubility, high-hydrophilic, can with polyvalent cation as Ca
2+, Ba
2+and Fe
3+the gel (Smidsr dO, the SkjaG. that form three-dimensional net structure is combined Deng interaction and the G unit in sodium alginate chemical chain
trendsinBiotechnology, 1990,8,71-78), and to natural extracellular matrix, there is similar structure, biocompatibility is good.
In addition, normal some antibacterials of interpolation in wound repair material, make it at wound circumference slow releasing, control inflammation reaction, protects from infection, to promote the regeneration of tissue.Select have the medicines such as the plant substitute antibiotics of pharmacologically active be just subject to increasing concern (WangQ, HuX, DuY,
etal.
carbohydratePolymers, 2010,82,842-847).In recent years, Aloe (AloeVera, A) causes the broad interest of researcher with the antibacterial advantage of its 100% pure natural, and this is due to its ingredient mainly (HammanJH. such as aminoacid, enzyme, vitamin and polysaccharide
molecules, 2008,13,1599-1616), there is significant pharmacologically active, effective to the treatment of various diseases, be widely used in production (SharrifMoghaddasiM, the VermaSK. of folk medicine, biomedicine, pharmacy and cosmetics
intJBiolMedRes, 2011,2,466-471); Study simultaneously and also find, Aloe also has antiinflammatory, antioxidation and promotes the functions such as wound healing, and these all prove that it can meet the needs (RinaudoM. for the treatment of dermatosis
progressinPolymerScience, 2006,31,603-632; SilvaSS, ManoJF, ReisRL.
biomimeticApproachesforBiomaterialsDevelopment, 2012,537-557).
Summary of the invention
The object of this invention is to provide one and prepare Aloe/sodium alginate Double-layer water gel dressing by simple green syt process, make it have suitable mechanical property, have the ability of good anti-microbial property and promotion fibroblast proliferation simultaneously concurrently.
The present invention with pure natural macromolecular material sodium alginate be raw material, natural drug Aloe is additive, with calcium chloride for cross-linking agent, after the preparation of monolayer hydrogel thin film, the compound of Double-layer water gel film and follow-up crosslinked and drying, obtain and there is the ultrathin aerogel dressing of bilayer that is colourless, high light transmittance, it can effectively promote fibroblastic propagation, weak sensitization and nonirritant are presented to skin, and there is excellent anti-microbial property, in Native Gel dressing clinical practice field, there is huge market prospect.
A preparation method for Aloe/sodium alginate Double-layer water gel dressing, is characterized in that the method comprises the following steps:
1) preparation of nontoxic basal layer hydrogel thin film
In sodium alginate aqueous solution, add plasticiser glycerol, stir until dissolve completely, ultrasonicly carry out degassed process, room temperature leaves standstill and obtains sodium alginate gel, is then instiled in culture dish, is drying to obtain sodium alginate basal layer;
2) preparation of the upper water gel of medicine is loaded with
Add in sodium alginate aqueous solution by aloe water solution, stir, supersound process, room temperature leaves standstill and obtains Aloe/sodium alginate mixed gel;
3) preparation of Double-layer water gel film
Aloe/sodium alginate mixed gel is instiled in sodium alginate substrate surface, dry;
4) Double-layer water gel film is crosslinked
Step 3) gained Double-layer water gel film is immersed CaCl
25-20min in solution, then with distilled water flushing, be drying to obtain Aloe/sodium alginate Double-layer water gel dressing.
In described sodium alginate aqueous solution, the mass volume ratio of sodium alginate powder and distilled water is 1:20 ~ 1:200g/mL.
The mass ratio of described plasticiser glycerol and sodium alginate powder is 0.6 ~ 6:1.
Described plasticiser glycerol adds in whipping process.
The amount that described step 1) and step 3) instil is the height that liquid is formed in culture dish is 0.5cm-2cm.
The condition of described step 1) and step 3) drying is 37 ~ 60 DEG C, relative humidity 50%.
In described aloe water solution, the mass volume ratio of Aloe and distilled water is 1:20 ~ 5:20g/mL.
Described step 2) mass ratio of Aloe and sodium alginate is 1:1,3:1 or 5:1 in mixed gel, SA and A is respectively the abbreviation of sodium alginate and Aloe, different quality ratio (mass ratio of A/SA is respectively: SA/A complex 0,1,3,5) is called after SA, SA1A1, SA1A3, SA1A5 respectively).
The present invention adopts easy method to prepare to have the ultra-thin aerogel dressing of colourless bilayer of high transmission rate (see table 1) and high swelling ratio; In addition, this dressing also has excellent anti-microbial property and promotes the ability of fibroblast proliferation, and presents weak sensitization and nonirritant to skin.The performance of these excellences makes it in clinical practice, have larger potential.
Table 1 is the performance of Double-layer water gel dressing
Accompanying drawing explanation
Fig. 1 is the light transmission (a) of aerogel dressing and adaptive capacity (b) photo with human body skin.
Fig. 2 is pure sodium alginate, the infrared spectrogram of Alose.
Fig. 3 is the infrared spectrogram of Double-layer water gel dressing SA, SA1A1, SA1A3, SA1A5.
Fig. 4 is that Double-layer water gel dressing SA, SA1A1, SA1A3, SA1A5 are to the optical photograph of escherichia coli (a) with staphylococcus aureus (b) antibacterial action.In figure 0,5,15,25 represents that the concentration of Aloe in Double-layer water gel dressing SA, SA1A1, SA1A3, SA1A5 is 0,5%, 15%, 25% respectively.
Fig. 5 is the fluorescent microscopy images of Double-layer water gel dressing SA, SA1A1, SA1A3, SA1A5 Surface L 929 cell.
Fig. 6 is the dermoreaction contrast figure of SD rat intact skin group before and after hydrogel material stimulates.
Fig. 7 is the dermoreaction contrast figure of SD rat damaged skin group before and after hydrogel material stimulates.
Fig. 8 is the dermoreaction contrast figure before and after SD rat skin sensitization.
Detailed description of the invention
Embodiment 1
The preparation of a nontoxic basal layer Sodium Alginate Hydrogel Films thin film
Be dissolved in by the sodium alginate powder of 0.5g in 100mL distilled water, stir under room temperature, until dissolve completely, add 0.6mL plasticiser glycerol, aerofluxus 12h in whipping process, room temperature leaves standstill.Getting a certain amount of sodium alginate gel afterwards instils in culture dish, and the height of formation is 0.5cm, temperature be 37 DEG C, relative humidity is dry under being the condition of 50%, at least 24h.
B is loaded with the preparation of the upper water gel of medicine
The Aloe of 5g is dissolved in 100mL distilled water, stirs under room temperature, until dissolve completely; According to different quality ratio, (mass ratio of A/SA is respectively: 1,3,5) again, appropriate aloe solution is joined in sodium alginate soln, makes the Aloe/sodium alginate mixed liquor with different quality ratio respectively, stirring reaction 2h under room temperature, aerofluxus 24h, room temperature stand for standby use.
C different quality is than the preparation of Double-layer water gel film
The mixed solution of different quality ratio is instiled in the Sodium Alginate Hydrogel Films film surface of drying, 37 DEG C, dry under the condition of relative humidity 50%, at least 24h.
D different quality being cross-linked than Double-layer water gel film
Take out after dry 24h, the Double-layer water gel film obtained by step c immerses the CaCl of 5%
2in solution, crosslinked 5min, is rinsed for several times with distilled water, drying at room temperature 24h, is preserved.
The biology performance test of Double-layer water gel film
Select escherichia coli (E.coli) and staphylococcus aureus (S.aureus), agar diffusion method is adopted to carry out anti-microbial property test to above-mentioned product, when its content is greater than the mass ratio of 15%(A and SA higher than 3) time, this hydrogel material has significant antibiotic property (Fig. 4); By above-mentioned product and L929 l cell Dual culture, by propagation and the adhesion of MTT colorimetry and immunofluorescence observation of cell, the hydrogel thin film containing Aloe can promote propagation and the adhesion (Fig. 5) of L929 cell; By adopting SD rat to evaluate above-mentioned product to the zest of skin and sensitization, the rat skin smearing above-mentioned product is non-stimulated, anaphylaxis.That is, this aerogel dressing is to no skin irritation (Fig. 6,7), weak sensitization (Fig. 8)
Embodiment 2
The sodium alginate powder of 2.5g is dissolved in 100mL distilled water by a, stirs under room temperature, until dissolve completely, adds 1.2mL plasticiser glycerol, aerofluxus 12h in whipping process, and room temperature leaves standstill.Getting a certain amount of sodium alginate gel afterwards instils in culture dish, and the height of formation is 0.5cm, temperature be 37 DEG C, relative humidity is dry under being the condition of 50%, at least 24h.
B is loaded with the preparation of the upper water gel of medicine
The Aloe of 15g is dissolved in 100mL distilled water, stirs under room temperature, until dissolve completely; According to different quality ratio, (mass ratio of A/SA is respectively: 1,3,5) again, appropriate aloe solution is joined in sodium alginate soln, makes the Aloe/sodium alginate mixed liquor with different quality ratio respectively, stirring reaction 2h under room temperature, aerofluxus 24h, room temperature stand for standby use.
C different quality is than the preparation of Double-layer water gel film
With embodiment 1.
D different quality being cross-linked than Double-layer water gel film
With embodiment 1.
The biology performance test of Double-layer water gel film
With embodiment 1.
Embodiment 3
The sodium alginate powder of 5g is dissolved in 100mL distilled water by a, stirs under room temperature, until dissolve completely, adds 6mL plasticiser glycerol, aerofluxus 12h in whipping process, and room temperature leaves standstill.Getting a certain amount of sodium alginate gel afterwards instils in culture dish, forms the height of 0.5cm, temperature be 37 DEG C, relative humidity is dry under being the condition of 50%, at least 24h.
B is loaded with the preparation of the upper water gel of medicine
The Aloe of 25g is dissolved in 100mL distilled water, stirs under room temperature, until dissolve completely; According to different quality ratio, (mass ratio of A/SA is respectively: 1,3,5) again, appropriate aloe solution is joined in sodium alginate soln, makes the Aloe/sodium alginate mixed liquor with different quality ratio respectively, stirring reaction 2h under room temperature, aerofluxus 24h, room temperature stand for standby use.
C different quality is than the preparation of Double-layer water gel film
With embodiment 1.
D different quality being cross-linked than Double-layer water gel film
With embodiment 1.
The biology performance test of Double-layer water gel film
With embodiment 1.
Claims (8)
1. a preparation method for Aloe/sodium alginate Double-layer water gel dressing, is characterized in that the method comprises the following steps:
1) preparation of nontoxic basal layer hydrogel thin film
In sodium alginate aqueous solution, add plasticiser glycerol, stir until dissolve completely, ultrasonic, room temperature leaves standstill and obtains sodium alginate gel, is then instiled in culture dish, is drying to obtain sodium alginate basal layer;
2) preparation of the upper water gel of medicine is loaded with
Add in sodium alginate aqueous solution by aloe water solution, stir, supersound process, room temperature leaves standstill and obtains Aloe/sodium alginate mixed gel;
3) preparation of Double-layer water gel film
Aloe/sodium alginate mixed gel is instiled in sodium alginate substrate surface, dry;
4) Double-layer water gel film is crosslinked
Step 3) gained Double-layer water gel film is immersed CaCl
25-20min in solution, then with distilled water flushing, be drying to obtain Aloe/sodium alginate Double-layer water gel dressing.
2. preparation method as claimed in claim 1, is characterized in that the mass volume ratio of sodium alginate powder and distilled water in described sodium alginate aqueous solution is 1:20 ~ 1:200g/mL.
3. preparation method as claimed in claim 1, is characterized in that the mass ratio of described plasticiser glycerol and sodium alginate powder is 0.6 ~ 6:1.
4. preparation method as claimed in claim 1, is characterized in that described plasticiser glycerol adds in whipping process.
5. preparation method as claimed in claim 1, is characterized in that amount that described step 1) and step 3) instil be the height that liquid is formed in culture dish is 0.5cm-2cm.
6. preparation method as claimed in claim 1, the condition of described step 1) and step 3) drying that it is characterized in that is 37 ~ 60 DEG C, relative humidity 50%.
7. preparation method as claimed in claim 1, is characterized in that the mass volume ratio of Aloe and distilled water in described aloe water solution is 1:20 ~ 5:20g/mL.
8. preparation method as claimed in claim 1, is characterized in that described step 2) mass ratio of Aloe and sodium alginate is 1:1,3:1 or 5:1 in mixed gel.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107519535A (en) * | 2017-09-28 | 2017-12-29 | 武汉理工大学 | Degradable sodium alginate/fibroin bilayer skin tissue engineering bracket material and preparation method thereof |
| CN108434503A (en) * | 2018-05-24 | 2018-08-24 | 东莞市联洲知识产权运营管理有限公司 | A kind of environment-friendly type medical dressing of antibacterial hemostasis and preparation method thereof |
| CN109432065A (en) * | 2018-12-25 | 2019-03-08 | 福建农林大学 | A kind of stealthy net acne patch of konjaku glucomannan |
| CN110548214A (en) * | 2019-08-26 | 2019-12-10 | 北京理工大学 | preparation method of miniature intelligent calcium alginate hydrogel end manipulator |
| CN111440349A (en) * | 2020-06-01 | 2020-07-24 | 中国石油大学(华东) | Preparation method of sodium alginate antibacterial dressing |
| CN112675359A (en) * | 2020-12-31 | 2021-04-20 | 无锡中科光远生物材料有限公司 | Sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film and preparation method thereof |
| CN113604461A (en) * | 2021-08-13 | 2021-11-05 | 合肥卡迪尔生物科技有限公司 | Immobilized enzyme and preparation method and application thereof |
| CN115572414A (en) * | 2021-07-06 | 2023-01-06 | 江南大学 | Anti-freezing heat-resistant hydrogel forming method |
| CN115569232A (en) * | 2022-10-31 | 2023-01-06 | 湖北三江航天江河化工科技有限公司 | Double-layer hydrogel dressing and preparation method and application thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107519535B (en) * | 2017-09-28 | 2020-05-05 | 武汉理工大学 | Degradable sodium alginate/fibroin double-layer skin tissue engineering scaffold material and preparation method thereof |
| CN107519535A (en) * | 2017-09-28 | 2017-12-29 | 武汉理工大学 | Degradable sodium alginate/fibroin bilayer skin tissue engineering bracket material and preparation method thereof |
| CN108434503A (en) * | 2018-05-24 | 2018-08-24 | 东莞市联洲知识产权运营管理有限公司 | A kind of environment-friendly type medical dressing of antibacterial hemostasis and preparation method thereof |
| CN109432065A (en) * | 2018-12-25 | 2019-03-08 | 福建农林大学 | A kind of stealthy net acne patch of konjaku glucomannan |
| CN110548214A (en) * | 2019-08-26 | 2019-12-10 | 北京理工大学 | preparation method of miniature intelligent calcium alginate hydrogel end manipulator |
| CN111440349B (en) * | 2020-06-01 | 2022-05-27 | 中国石油大学(华东) | Preparation method of sodium alginate antibacterial dressing |
| CN111440349A (en) * | 2020-06-01 | 2020-07-24 | 中国石油大学(华东) | Preparation method of sodium alginate antibacterial dressing |
| CN112675359A (en) * | 2020-12-31 | 2021-04-20 | 无锡中科光远生物材料有限公司 | Sodium alginate-hydroxyapatite hybrid nanoparticle double-layer film and preparation method thereof |
| CN115572414A (en) * | 2021-07-06 | 2023-01-06 | 江南大学 | Anti-freezing heat-resistant hydrogel forming method |
| CN115572414B (en) * | 2021-07-06 | 2024-01-05 | 江南大学 | Method for forming anti-freezing heat-resistant hydrogel |
| CN113604461A (en) * | 2021-08-13 | 2021-11-05 | 合肥卡迪尔生物科技有限公司 | Immobilized enzyme and preparation method and application thereof |
| CN115569232A (en) * | 2022-10-31 | 2023-01-06 | 湖北三江航天江河化工科技有限公司 | Double-layer hydrogel dressing and preparation method and application thereof |
| CN115569232B (en) * | 2022-10-31 | 2023-07-18 | 湖北三江航天江河化工科技有限公司 | Double-layer hydrogel dressing and preparation method and application thereof |
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