CN105075839A - Water planting apparatus and water planning method for large-scale wheat drought resistance simulation - Google Patents

Water planting apparatus and water planning method for large-scale wheat drought resistance simulation Download PDF

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Publication number
CN105075839A
CN105075839A CN201510531227.3A CN201510531227A CN105075839A CN 105075839 A CN105075839 A CN 105075839A CN 201510531227 A CN201510531227 A CN 201510531227A CN 105075839 A CN105075839 A CN 105075839A
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black
turnover box
hydroponic device
wheat
storage container
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CN201510531227.3A
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CN105075839B (en
Inventor
盖红梅
崔健
李玉刚
孙绿
赵长星
王圣健
李振清
迟红梅
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QINGDAO INSTITUTE OF AGRICULTURAL SCIENCES
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QINGDAO INSTITUTE OF AGRICULTURAL SCIENCES
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

The present invention provides a water planting apparatus and a water planning method for large-scale wheat drought resistance simulation. The apparatus comprises: black turnover boxes, floating seedling growing discs, a liquid storage container, a water pump and a timing control apparatus; a liquid inlet pipe of the water pump is communicated with the liquid storage container; a liquid transmission header pipe of the water pump is provided with a plurality of liquid transmission branch pipes; a liquid transmission port of each liquid transmission branch pipe is positioned over the corresponding black turnover box; and the same height position of each black turnover box is provided with a water outlet, the water outlets of the black turnover boxes are serially connected via a drainage pipeline, and a drainage end of the drainage pipeline is communicated with the liquid storage container. The water planting apparatus and the water planning method have the advantages that nutrient solution and hypertonic solution respectively cycle and flow, oxygen content is ensured, the nutrient solution and the hypertonic solution are not required to be frequently replaced, so that manpower and material resources are reduced; and in addition, consistency of the nutrient solution and the hypertonic solution among different turnover boxes is improved, consistent growing environment is provided for different wheat materials, and identification and evaluation of large-scale wheat seedling stage drought resistance simulation are achieved.

Description

Carry out hydroponic device and the ciltivating process of wheat simulation drought resisting on a large scale
Technical field
The invention belongs to agricultural science and technology technical field, be specifically related to a kind of hydroponic device and the ciltivating process of carrying out wheat simulation drought resisting on a large scale.
Background technology
Drought stress is one of main abiotic stress harmed the crops, in order to explore Drought-resistance in Wheat mechanism, hypertonic solution method is adopted to process wheat seedling, cause the physiological drought of crop, combining form and physiological index determining again, the final drought resistance evaluating Wheat Seedling is the method that numerous scholar adopts.
In prior art, the following two kinds of methods of main employing carry out the research of drought resistance to wheat seedling: first method: adopt triangular flask as container, in control group, use ordinary nutritional liquid to cultivate wheat seedling; In experimental group, hypertonic solution is adopted to cultivate wheat seedling; Then by comparing the growth conditions of the wheat seedling of control group and experimental group, and then the drought resistance of wheat seedling is evaluated; The maximum limitation of triangular flask cultural method is: the quantity of the wheat seedling that each triangular flask is cultivated very little, is unfavorable for the research carrying out wheat simulation drought resisting on a large scale; In addition, because nutrient solution and hypertonic solution use are after 3-5 days, the oxygen content of nutrient solution and hypertonic solution can be caused to reduce, therefore, for preventing wheat root from rotting because of anoxic, needing within 3-5 days, regularly replace nutrient solution and hypertonic solution, wasting operating personnel's plenty of time and energy thus; Second method: adopt cultivation box or Turnover Box as container, in control group, uses ordinary nutritional liquid to cultivate wheat seedling; In experimental group, hypertonic solution is adopted to cultivate wheat seedling; Although this kind of processing method improves wheat seedling and cultivates quantity, there is the problem needing to regularly replace nutrient solution and hypertonic solution equally, waste a large amount of man power and materials.
Therefore, relevant culture vessel of the prior art and cultural method, all cannot realize the extensive simulation drought resisting research of wheat.
Summary of the invention
For the defect that prior art exists, the invention provides a kind of hydroponic device and the ciltivating process of carrying out wheat simulation drought resisting on a large scale, can effectively solve the problem.
The technical solution used in the present invention is as follows:
The invention provides a kind of hydroponic device carrying out wheat simulation drought resisting on a large scale, comprising: n black Turnover Box, a n floating seedling tray, liquid storage container, suction pump and timing control apparatus; Wherein, n is natural number;
Masking foil is pasted in the bottom of each described floating seedling tray, offers through hole at the correspondence position of the position, each hole with described floating seedling tray;
Each floating seedling tray having pasted masking foil is placed in unique corresponding black Turnover Box;
The feed tube of described suction pump is communicated with described liquid storage container; The transfusion house steward of described suction pump is upper draws several transfusion arms, and the infusion port of each described transfusion arm is positioned at the top of described black Turnover Box, by the hole of floating seedling tray, injects liquid to described black Turnover Box; Offer discharge outlet in the sustained height position of each described black Turnover Box, described in each, the discharge outlet of black Turnover Box is connected by drainage pipeline, and the discharge ends of described drainage pipeline is communicated with described liquid storage container;
In addition, described timing control apparatus is electrically connected with described suction pump.
Preferably, described black Turnover Box is the cuboid container of outside dimension: 66*40*16cm, inside dimension: 62*36*15cm.
Preferably, described floating seedling tray is 132 hole floating seedling trays.
The present invention also provides a kind of ciltivating process carrying out wheat simulation drought resisting on a large scale, comprises the following steps:
Step 1: build control group hydroponic device and experimental group hydroponic device;
Step 2: preparation nutrient solution; Quantitative nutrient solution is injected in the black Turnover Box and liquid storage container of control group hydroponic device; Meanwhile, in the black Turnover Box and liquid storage container of experimental group hydroponic device, inject the nutrient solution of equivalent;
Step 3: the wheat seedling proceeding to 8 ~ 12 days in the floating seedling tray of control group hydroponic device; Meanwhile, in the floating seedling tray of experimental group hydroponic device, proceed to the wheat seedling of 8 ~ 12 days;
Step 4, is set to the duration after identical timing and each startup by the timing control apparatus of control group hydroponic device and experimental group hydroponic device; Then, the suction pump of control group hydroponic device and experimental group hydroponic device is started respectively;
Wherein, for control group hydroponic device and experimental group hydroponic device, after suction pump starts, the nutrient solution in liquid storage container is transported in each black Turnover Box by suction pump respectively; Meanwhile, because the top equal altitudes of black Turnover Box is provided with discharge outlet, therefore, when the water yield stored in black Turnover Box reaches discharge outlet height, just flowed out by discharge outlet, and finally flow back in liquid storage container, realize the periodic cyclic of nutrient solution thus;
Step 5, after incubation time reaches setting-up time, control group hydroponic device is pressed proterotype and is continued to run; And experimental group, then need to add quantitative PEG-6000 in liquid storage container and black Turnover Box, make it reach-0.5MPa, make it transfer hypertonic solution to from nutrient solution; And by circulation pattern circulation time original, make the solution state in each Turnover Box consistent;
After certain incubation time, by morphological characters and the physical signs of the Wheat Seedling of comparative test group hydroponic device and control group hydroponic device, realize Wheat Seedling drought resisting evaluation.
Preferably, in step 3, when proceeding to wheat seedling in floating seedling tray, adopt sponge to be fixed wheat seedling simultaneously.
Preferably, in step 4, timing is: 1.5 hours to 3.5 hours; Duration after each startup is 20-40 minute.
Herein, according to actual needs, flexibly adjustment timing and the duration after at every turn starting, as a kind of preferred embodiment, every day can be undertaken by following setting:
Cycle-index and duration
Preferably, in step 5, setting-up time is 14 days.
Provided by the inventionly carry out the wheat simulation hydroponic device of drought resisting on a large scale and ciltivating process has the following advantages:
Carrying out in wheat seedling incubation, nutrient solution and hypertonic solution are being circulated respectively, ensures its oxygen content, without the need to frequently changing nutrient solution and hypertonic solution, thus reducing manpower and materials; In addition, nutrient solution or the hypertonic solution of each black Turnover Box are all communicated with liquid storage container, thus add the uniformity of nutrient solution and hypertonic solution between different black Turnover Box, for different wheat lines provides consistent growing environment, realize qualification and the evaluation of extensive Wheat Seedling simulation drought resisting.
Accompanying drawing explanation
Fig. 1 is the structural representation carrying out the hydroponic device of wheat simulation drought resisting on a large scale provided by the invention;
In FIG, 1 liquid storage container is represented; 2,3 and 4 the 1st black Turnover Box, the 2nd black Turnover Box and the 3rd black Turnover Box is represented respectively; 5 representative transfusion house stewards; 6 represent drainage pipeline.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in detail:
The present invention is directed in the research of wheat simulating drought, when using hypertonic solution and nutrient solution to cultivate wheat, need repeatedly to change nutrient solution and hypertonic solution, cause operating personnel's workload too large thus, and then the problem of extensive wheat germplasm drought resisting evaluation cannot be realized, propose wheat water planting culture apparatus and cultural method that a kind of nutrient solution and hypertonic solution can circulate, thus decrease manpower and materials, add the uniformity of different black Turnover Box nutrient solution and hypertonic solution, extensive Wheat Seedling simulation drought resisting qualification can be realized and evaluate, particular content is as follows:
Composition graphs 1, the invention provides a kind of hydroponic device carrying out wheat simulation drought resisting on a large scale, comprising: n black Turnover Box, a n floating seedling tray, liquid storage container, suction pump and timing control apparatus; Wherein, n is natural number; In practical application, the magnitude setting of black Turnover Box is determined according to the quantity of testing sample, such as, is dozens of; Further, black Turnover Box can adopt outside dimension: the cuboid container of 66*40*16cm, inside dimension: 62*36*15cm.The quantity of floating seedling tray is identical with the magnitude setting of black Turnover Box, and can adopt 132 hole floating seedling trays, certainly, the hole figure place of floating seedling tray can carry out suitable adjustment according to the quantity of material.In FIG, the structure of not shown floating seedling tray; Further, in FIG, to arrange 3 black Turnover Boxes for exemplifying.
Masking foil is pasted, to ensure the black culture environment of wheat root in the bottom of each described floating seedling tray; Further, on described masking foil, through hole is offered with the correspondence position of the position, each hole of described floating seedling tray; Ensure in the hypertonic solution that wheat root can extend into black Turnover Box or nutrient solution.
Each floating seedling tray having pasted masking foil is placed in unique corresponding black Turnover Box;
A hollow place can be dug in ground near black Turnover Box, then, liquid storage container is inserted in hollow place, liquid storage container can be bucket, volume is determined according to actual testing requirement, such as, is 100L etc., described suction pump can be arranged in liquid storage container, and the feed tube of suction pump is communicated with described liquid storage container; The transfusion house steward of described suction pump is upper draws several transfusion arms, and the infusion port of each described transfusion arm is positioned at the top of described black Turnover Box, the liquid in liquid storage container can be injected into described black Turnover Box; Offer discharge outlet in the sustained height position of each described black Turnover Box, discharge outlet is opened in the top position of black Turnover Box, for when in black Turnover Box, injected water volume reaches setting value, by discharge outlet overflow.Described in each, the discharge outlet of black Turnover Box is connected by drainage pipeline, and the discharge ends of described drainage pipeline is communicated with described liquid storage container; Can ensure that the liquid after each black Turnover Box overflow turns back to liquid storage container, realize circulating of liquid.
In addition, described timing control apparatus is electrically connected with described suction pump, for the start and stop of timing controlled suction pump, and then realizes the efficient circulation flowing of hypertonic solution or nutrient solution.
The present invention also provides a kind of ciltivating process carrying out wheat simulation drought resisting on a large scale, comprises the following steps:
Step 1: build control group hydroponic device and experimental group hydroponic device;
Wherein, control group hydroponic device and experimental group hydroponic device are the hydroponic device introduced above, and difference is only that control group hydroponic device is different with the liquid that experimental group hydroponic device adopts.
Step 2: preparation nutrient solution; Quantitative nutrient solution is injected in the black Turnover Box and liquid storage container of control group hydroponic device; Meanwhile, in the black Turnover Box and liquid storage container of experimental group hydroponic device, inject the nutrient solution of equivalent;
Step 3: the wheat seedling proceeding to 8 ~ 12 days in the floating seedling tray of control group hydroponic device; Meanwhile, in the floating seedling tray of experimental group hydroponic device, proceed to the wheat seedling of 8 ~ 12 days; In addition, when proceeding to wheat seedling in floating seedling tray, adopt sponge to be fixed wheat seedling simultaneously.
Step 4, is set to the duration after identical timing and each startup by the timing control apparatus of control group hydroponic device and experimental group hydroponic device; Then, the suction pump of control group hydroponic device and experimental group hydroponic device is started respectively;
Wherein, for control group hydroponic device and experimental group hydroponic device, after suction pump starts, the nutrient solution in liquid storage container is transported in each black Turnover Box by suction pump respectively; Meanwhile, because the top equal altitudes of black Turnover Box is provided with discharge outlet, therefore, when the water yield stored in black Turnover Box reaches discharge outlet height, just flowed out by discharge outlet, and finally flow back in liquid storage container, realize the periodic cyclic of nutrient solution thus; By periodic cyclic nutrient solution, ensure that the oxygen content of nutrient solution, thus do not need frequently to change nutrient solution.
Step 5, after incubation time reaches setting-up time, control group hydroponic device is pressed proterotype and is continued to run; And experimental group, then need to add quantitative PEG-6000 in liquid storage container and black Turnover Box, make it reach-0.5MPa, make it transfer hypertonic solution to from nutrient solution; And by circulation pattern circulation time original, make the solution state in each Turnover Box consistent;
After certain incubation time, by morphological characters and the physical signs of the Wheat Seedling of comparative test group hydroponic device and control group hydroponic device, realize Wheat Seedling drought resisting evaluation.
In addition, following comparative example is carried out:
Test example: for hydroponic device provided by the invention take nutrient solution as medium, adopts preceding method, and suction pump started once every 3 hours, and then made nutrient solution periodic cyclic, after 14 days, observes morphological characters and the physical signs of Wheat Seedling.
Reference examples: adopt conventional cultural method, that is: to cultivate box for container, adopt and the identical nutrient solution of test example, and, the wheat seedling identical with test example Wheat Seedling is adopted to cultivate, change one time of nutrition liquid every 3 days, after 14 days, observe morphological characters and the physical signs of Wheat Seedling.
Through comparing, can see, test example cultivate morphological characters and the physical signs of the Wheat Seedling obtained, basically identical with reference examples.Prove the method that nutrient solution is circulated of the present invention thus, the culture effect basically identical with periodic replacement nutrient solution can be reached.But, can obviously find out, equipment provided by the present invention and method, frequent trouble and the physical waste of changing nutrient solution and hypertonic solution can be removed from, the such as Turnover Box of a 40*66, can hold nutrient solution 20L, i.e. the weight of about 20 kilograms, it is just very difficult that general schoolgirl does this experiment, and boy student also needs to expend many muscle power.Moreover, each Turnover Box nutrient solution used needs macronutrient, comprises a large amount of, middle amount and trace element, processed group also needs PEG-6000, and these medicines all need higher cost, and the present invention, by recycling nutrient solution and hypertonic solution, save a lot of drug expenditure.Therefore, the present invention obviously saves the expense that wheat simulation drought resisting is cultivated, the time of saving time and the replacing nutrient solution prepared medicines in a large number.The present inventor utilizes the inventive method, and nutrient solution and the hypertonic solution of having carried out 120 parts of wheat germplasms are cultivated, and obtain more than 20,000 physiology and morphology characteristic index, and can study the root traits of wheat under different disposal condition easily.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should look protection scope of the present invention.

Claims (7)

1. carry out a hydroponic device for wheat simulation drought resisting on a large scale, it is characterized in that, comprising: n black Turnover Box, a n floating seedling tray, liquid storage container, suction pump and timing control apparatus; Wherein, n is natural number;
Masking foil is pasted in the bottom of each described floating seedling tray, offers through hole at the correspondence position of the position, each hole with described floating seedling tray;
Each floating seedling tray having pasted masking foil is placed in unique corresponding black Turnover Box;
The feed tube of described suction pump is communicated with described liquid storage container; The transfusion house steward of described suction pump is upper draws several transfusion arms, and the infusion port of each described transfusion arm is positioned at the top of described black Turnover Box, by the hole of floating seedling tray, injects liquid to described black Turnover Box; Offer discharge outlet in the sustained height position of each described black Turnover Box, described in each, the discharge outlet of black Turnover Box is connected by drainage pipeline, and the discharge ends of described drainage pipeline is communicated with described liquid storage container;
In addition, described timing control apparatus is electrically connected with described suction pump.
2. the hydroponic device carrying out wheat simulation drought resisting on a large scale according to claim 1, it is characterized in that, described black Turnover Box is the cuboid container of outside dimension: 66*40*16cm, inside dimension: 62*36*15cm.
3. the hydroponic device carrying out wheat simulation drought resisting on a large scale according to claim 1, it is characterized in that, described floating seedling tray is 132 hole floating seedling trays.
4. carry out a ciltivating process for wheat simulation drought resisting on a large scale, it is characterized in that, comprise the following steps:
Step 1: build control group hydroponic device and experimental group hydroponic device;
Step 2: preparation nutrient solution; Quantitative nutrient solution is injected in the black Turnover Box and liquid storage container of control group hydroponic device; Meanwhile, in the black Turnover Box and liquid storage container of experimental group hydroponic device, inject the nutrient solution of equivalent;
Step 3: the wheat seedling proceeding to 8 ~ 12 days in the floating seedling tray of control group hydroponic device; Meanwhile, in the floating seedling tray of experimental group hydroponic device, proceed to the wheat seedling of 8 ~ 12 days;
Step 4, is set to the duration after identical timing and each startup by the timing control apparatus of control group hydroponic device and experimental group hydroponic device; Then, the suction pump of control group hydroponic device and experimental group hydroponic device is started respectively;
Wherein, for control group hydroponic device and experimental group hydroponic device, after suction pump starts, the nutrient solution in liquid storage container is transported in each black Turnover Box by suction pump respectively; Meanwhile, because the top equal altitudes of black Turnover Box is provided with discharge outlet, therefore, when the water yield stored in black Turnover Box reaches discharge outlet height, just flowed out by discharge outlet, and finally flow back in liquid storage container, realize the periodic cyclic of nutrient solution thus;
Step 5, after incubation time reaches setting-up time, control group hydroponic device is pressed proterotype and is continued to run; And experimental group, then need to add quantitative PEG-6000 in liquid storage container and black Turnover Box, make it reach-0.5MPa, make it transfer hypertonic solution to from nutrient solution; And by circulation pattern circulation time original, make the solution state in each Turnover Box consistent;
After certain incubation time, by morphological characters and the physical signs of the Wheat Seedling of comparative test group hydroponic device and control group hydroponic device, realize Wheat Seedling drought resisting evaluation.
5. the ciltivating process carrying out wheat simulation drought resisting on a large scale according to claim 4, is characterized in that, in step 3, when proceeding to wheat seedling in floating seedling tray, adopts sponge to be fixed wheat seedling simultaneously.
6. the ciltivating process carrying out wheat simulation drought resisting on a large scale according to claim 4, it is characterized in that, in step 4, timing is: 1.5 hours to 3.5 hours; Duration after each startup is 20-40 minute.
7. the ciltivating process carrying out wheat simulation drought resisting on a large scale according to claim 4, it is characterized in that, in step 5, setting-up time is 14 days.
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CN105557487A (en) * 2015-12-22 2016-05-11 山东农业大学 Test method for studying high-temperature stress on wheat
CN106973773A (en) * 2017-05-10 2017-07-25 中国农业科学院烟草研究所 The extensive water planting device and method for carrying out tobacco secondary metabolites phenotypic evaluation
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CN106973773A (en) * 2017-05-10 2017-07-25 中国农业科学院烟草研究所 The extensive water planting device and method for carrying out tobacco secondary metabolites phenotypic evaluation
CN107114225A (en) * 2017-05-17 2017-09-01 漯河市农业科学院 A kind of wheat seedling cultural method

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