CN107114225A - A kind of wheat seedling cultural method - Google Patents
A kind of wheat seedling cultural method Download PDFInfo
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- CN107114225A CN107114225A CN201710346848.3A CN201710346848A CN107114225A CN 107114225 A CN107114225 A CN 107114225A CN 201710346848 A CN201710346848 A CN 201710346848A CN 107114225 A CN107114225 A CN 107114225A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Soil Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Inorganic Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of wheat seedling cultural method, including wheat vernalization；Using mandruka as medium carrier, culture matrix is injected into mandruka, sponge base culture medium is obtained；The structure of the mandruka is：Take block sponge to cut and dig out multiple seed channels, each seed channel is curve shape, and the outlet of the seed channel is located at the upper surface of block sponge, and the bottom of seed channel is located inside block sponge；Seedling culture is until wheat seedling grows up to.The present invention uses mandruka as culture medium carrier, it is to avoid sand culture base particle is scattered everywhere, it is ensured that with dioxygen water sterilization fully, and effectively preventing and treating wheat seed is gone mouldy, and moisture is not easily runed off and evaporated；The germination fluid and culture matrix used in seedling incubation can quickly break seed dormancy state, improve wheat germination efficiency.
Description
Technical field
The invention belongs to crops culture technique field, and in particular to a kind of wheat seedling cultural method.
Background technology
Wheat is the important crop products of China, and its yield, quality are by wheat breed, weather, soil, farming system
The influence of the conditions such as degree, cultural method.Because China human mortality is numerous, the demand every year to wheat is all very big, breeding high-yield,
The new variety of wheat of the merit such as disease-resistant, resistant to lodging is always the problem for enjoying scientist to pay close attention to.
Breeding of new variety of wheat method mainly include conventional hybridization breeding and it is short lose Genetic Sterility recurrent selection breeding and turn
Gene breeding etc., these breeding methods can all be related to wheat seedling culture experiment.Under normal circumstances, wheat seedling culture is used
Culture matrix be sand culture base, sand culture base is placed in culture dish by researcher, then by wheat seed culture
Into seedling.However, sand culture base particle is more, easily it is scattered everywhere, it is not easy to maintain, and also particulate matter sterilization is inconvenient,
Easily cause sterilization insufficient, ultimately result in wheat seed microbiological contamination and go mouldy.
The content of the invention
A kind of wheat seedling cultural method that the present invention is provided, is solved in the prior art, sand culture base particle is more,
Easily it is scattered everywhere, not easy to maintain, and particulate matter sterilization is inconvenient, it is insufficient to easily cause sterilization, ultimately results in wheat
The problem of seed microbiological contamination is gone mouldy.
It is an object of the invention to provide a kind of wheat seedling cultural method, comprise the following steps：
S1, wheat vernalization：The wheat seed for filtering out health carries out vernalization culture, obtains germinated wheat kind；
S2, is put into after mandruka is sterilized in the culture vessel after sterilization, using mandruka as medium carrier, Xiang Duo
Culture matrix is injected in the sponge of hole, sponge base culture medium is obtained；
The structure of the mandruka is：Take block sponge to cut and dig out multiple seed channels, each seed channel is bent
Wire shaped, and the outlet of the seed channel is located at the upper surface of block sponge, and the bottom of seed channel is located at block sponge
It is internal；
S3, seedling culture：Germinated wheat kind is put into seed channel, the mandruka of germinated wheat kind then will be filled
Full exposure culture in constant incubator is placed in, until wheat seedling grows up to；
Wherein, daily supplementing culture medium matter extremely submerges mandruka upper surface during full exposure culture.
It is preferred that, it is in the wheat seedling cultural method, the step of the vernalization culture：
(1) hydrogen peroxide dipping that wheat seed is placed in into volume fraction 1% is sterilized, and soak time is 30-60min, is obtained
Sterilize Wheat Species；
(2) sterilization Wheat Species are soaked in germination fluid, until wheat germination, obtains germinated wheat kind；
The germination fluid soaks juice according to 1 by the hydrogen peroxide and kuh-seng of volume fraction 1%:2 volume ratio is mixed；Its
In, kuh-seng leaching juice be by fresh kuh-seng boil, filter after collect obtained filtrate.
It is furthermore preferred that in the wheat seedling cultural method, the preparation method of 1L kuh-sengs leaching juice is：Take 100g clean it is new
Fresh kuh-seng, is cut into the sophora film of 0.5-1cm thickness, is then placed in water sophora film and boils 1.5-2h, filtering, collects and considers liquid,
1L finally is supplied with sterilized water, kuh-seng leaching juice is obtained.
It is preferred that, in the wheat seedling cultural method, the curve shape of seed channel is S-shaped shape in the mandruka
Or be 90 degree of arc shape.
It is preferred that, in the wheat seedling cultural method, the culture matrix is made up of the component of following concentration：0.5g/L
KNO3, 0.8g/L Ca (NO3)2, 0.2g/L NH4H2PO4, 0.10g/L MgSO4, 0.10g/L gibberellin, solvent is
Running water.
It is preferred that, in the wheat seedling cultural method, mandruka and culture vessel are using the double of volume fraction 1%
Oxygen water soaking disinfection, soak time is 10-20min.
Compared with prior art, a kind of wheat seedling cultural method that this practicality invention is provided, has the advantages that：
1st, the present invention uses mandruka as culture medium carrier, has both been avoided that sand culture base particle was scattered, no everywhere
Defect easy to maintain, can guarantee that with dioxygen water sterilization fully, effectively preventing and treating wheat seed goes mouldy, and improves percentage of seedgermination again；And
Culture matrix is injected in mandruka, property that sponge sucks in water is strong, and moisture is not easily runed off and evaporated, reduce irrigation times, save water
Resources costs；The Curve Design simulation soil texture environment of seed channel, the seedling after sprouting grows along seed channel, is easy to pendulum
Operation is planted, seed is difficult flowing, is easy to observation to manage, and due to the setting of seed channel, be easy to entering for the liquid such as hydrogen peroxide
Go out, that accelerates sponge soaks speed.
2nd, wheat seed is handled with 1% hydrogen peroxide dipping during vernalization culture, can prevent and treat bacterium, disease that seed is carried in itself
Bacterium etc., prevents seed from going mouldy sick, improves seedling quality；On the other hand, 1% hydrogen peroxide, which can be played, breaks seed dormancy shape
State, reaches vernalization purpose.
3rd, 1% hydrogen peroxide and kuh-seng leaching juice are quickly broken seed dormancy state, improved as the germination fluid of sterilization Wheat Species
Wheat germination efficiency, shortens the time required to sprouting, its effect is more better than 1% hydrogen peroxide dipping.
Brief description of the drawings
Fig. 1 is the structural representation of mandruka of the present invention.
Description of reference numerals：1. block sponge, 2. seed channels, 3. germinated wheat kinds.
Embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings, but should not be construed as the limit of the present invention
System.The test method of unreceipted actual conditions, is generally operated according to normal condition in the following example.When embodiment provides numerical value
During scope, it should be appreciated that except non-invention is otherwise noted, any one between two end points and two end points of each number range
Individual numerical value can select.Unless otherwise defined, all technologies and scientific terminology and the art technology used in the present invention
The meaning that personnel are generally understood that is identical.
The wheat seedling cultural method of the present invention, including following examples：
Embodiment 1
S1, wheat vernalization：Filter out health, without going mouldy, unabroken wheat seed carry out vernalization culture, obtain germination small
Wheat seeds；
The step of vernalization culture is：
(1) hydrogen peroxide dipping that wheat seed is placed in into volume fraction 1% is sterilized, and soak time is 30-40min, is obtained
Sterilize Wheat Species；
(2) sterilization Wheat Species are soaked in germination fluid, until wheat germination, obtains germinated wheat kind；
The germination fluid soaks juice according to 1 by the hydrogen peroxide and kuh-seng of volume fraction 1%:2 volume ratio is mixed；Its
In, the preparation method of 1L kuh-sengs leaching juice is：The fresh kuh-seng for taking 100g clean, is cut into the sophora film of 0.5-1cm thickness, then will
Sophora film, which is placed in 500ml water, boils 1.5h, filtering, collects and considers liquid, finally supplies 1L with sterilized water, obtains kuh-seng leaching juice.
S2, is put into after mandruka is sterilized in the culture vessel after sterilization, using mandruka as medium carrier, Xiang Duo
Culture matrix in the sponge of hole after injection sterilizing, until culture matrix submergence mandruka upper surface, obtains sponge base culture medium；
Mandruka and culture vessel are sterilized using the hydrogen peroxide dipping of volume fraction 1%, and soak time is 20min.
The structure of the mandruka is as shown in figure 1, be：Take block sponge to cut and dig out multiple seed channels, each seed
Passage is curve shape, and the outlet of the seed channel is located at the upper surface of block sponge, the bottom position of seed channel
Inside block sponge；The curve shape is S-shaped shape or is 90 degree of arc shape.
S3, seedling culture：Germinated wheat kind is put into seed channel, germinated wheat kind slips into seed channel bottom, its
Culture matrix is fully absorbed, and then grows up to seedling, then the mandruka for filling germinated wheat kind is placed in constant incubator
Full exposure culture, until wheat seedling grows up to；
Wherein, daily supplementing culture medium matter extremely submerges mandruka upper surface during full exposure culture.
It should be noted that culture matrix is made up of the component of following concentration described in S2 and S3：0.5g/L KNO3、
0.8g/L Ca (NO3)2, 0.2g/L NH4H2PO4, 0.10g/L MgSO4, 0.10g/L gibberellin, solvent is running water；
121 DEG C of sterilising conditions, 15min.
Embodiment 2
S1, wheat vernalization：Filter out health, without going mouldy, unabroken wheat seed carry out vernalization culture, obtain germination small
Wheat seeds；
The step of vernalization culture is：
(1) hydrogen peroxide dipping that wheat seed is placed in into volume fraction 1% is sterilized, and soak time is 50-60min, is obtained
Sterilize Wheat Species；
(2) sterilization Wheat Species are soaked in germination fluid, until wheat germination, obtains germinated wheat kind；
The germination fluid soaks juice according to 1 by the hydrogen peroxide and kuh-seng of volume fraction 1%:2 volume ratio is mixed；Its
In, the preparation method of 1L kuh-sengs leaching juice is：The fresh kuh-seng for taking 100g clean, is cut into the sophora film of 0.5-1cm thickness, then will
Sophora film, which is placed in 500ml water, boils 2h, filtering, collects and considers liquid, finally supplies 1L with sterilized water, obtains kuh-seng leaching juice.
S2, is put into after mandruka is sterilized in the culture vessel after sterilization, using mandruka as medium carrier, Xiang Duo
Culture matrix in the sponge of hole after injection sterilizing, until culture matrix submergence mandruka upper surface, obtains sponge base culture medium；
Mandruka and culture vessel are sterilized using the hydrogen peroxide dipping of volume fraction 1%, and soak time is 10min.
The structure of the mandruka is as shown in figure 1, be：Take block sponge to cut and dig out multiple seed channels, each seed
Passage is curve shape, and the outlet of the seed channel is located at the upper surface of block sponge, the bottom position of seed channel
Inside block sponge；The curve shape is S-shaped shape or is 90 degree of arc shape.
S3, seedling culture：Germinated wheat kind is put into seed channel, germinated wheat kind slips into seed channel bottom, its
Culture matrix is fully absorbed, and then grows up to seedling, then the mandruka for filling germinated wheat kind is placed in constant incubator
Full exposure culture, until wheat seedling grows up to；
Wherein, daily supplementing culture medium matter extremely submerges mandruka upper surface during full exposure culture.
It should be noted that culture matrix is made up of the component of following concentration described in S2 and S3：0.5g/L KNO3、
0.8g/L Ca (NO3)2, 0.2g/L NH4H2PO4, 0.10g/L MgSO4, 0.10g/L gibberellin, solvent is running water；
121 DEG C of sterilising conditions, 15min.
First, the comparison of different seed-soaking liquids
In wheat seedling incubation, wheat vernalization and growth of seedling are peroxidase in important stage, wheat seed
POD etc. activity be seed sprout key, we determine Seed soaking, 1% hydrogen peroxide seed soaking, germination fluid seed soaking and
The wheat POD activity that 1% hydrogen peroxide-germination fluid is soaked seed jointly, wherein 1% hydrogen peroxide-germination fluid seed soaking is first by wheat seed
It is placed in 1% hydrogen peroxide and soaks 30min, then wheat seed is transferred in germination fluid and soaked, remaining group is directly by wheat
Seed, which is placed in clear water, 1% hydrogen peroxide or germination fluid, to be soaked, as a result as shown in table 1.After Seed soaking 72h, Wheat Species POD
Activity is minimum, is 373.6Umin-1·(gFW)-1, now wheat physiological status is observed, it germination phenomenon does not occur；1%
Hydrogen peroxide-germination fluid seed soaking 72h active the highests of POD, wheat germination rate (the Wheat Species subnumber germinateed in every 100) is
98%；And after the seed soaking of 1% hydrogen peroxide and germination fluid seed soaking 72h, germination percentage is respectively 65% and 90%, illustrates that 1% hydrogen peroxide can
Break seed dormancy state to play, reach vernalization purpose, 1% hydrogen peroxide and kuh-seng leaching juice are used as the sprouting for sterilizing Wheat Species
Liquid, quickly breaks seed dormancy state, improves wheat germination efficiency, shortens the time required to sprouting, its effect is than 1% hydrogen peroxide
Immersion is better.
Wheat seed POD activity (Umin under the conditions of the different wheat seed soaking of table 1-1·(gFW)-1)
2nd, different culture media matter compares
In order to compare the effect of different culture media matter, we prepare germinated wheat using the vernalization incubation step of embodiment 1
Kind, then germinated wheat kind is respectively placed in the culture matrix (sterile) of aseptic water and the present invention and cultivated, and daily
Each supplement aseptic water and the culture matrix (sterile) of the present invention, the emergence rate and height of seedling of observed and recorded wheat seedling, are tied
Fruit as shown in table 2, as a result shows, using the culture matrix culture wheat seedling of the present invention, and emergence rate and height of seedling are aobvious compared with clear water
Write and improve, effect is obvious.
The emergence rate and height of seedling of the different culture media matter wheat of table 2
3rd, different culture media carrier compares
Germinated wheat kind is prepared using the vernalization incubation step of embodiment 1, sand is then respectively adopted and mandruka is made
For culture medium carrier culture wheat seedling, the sterilization side of the culture matrix, wherein sand and mandruka of the present invention is during which supplemented
Method is 1% hydrogen peroxide dipping sterilization soak time 20min, and the microbiological contamination of observation wheat seedling is gone mouldy situation, and calculating goes mouldy hundred
Point rate (seed amount that 100 grain germination Wheat Species go mouldy) result as shown in table 3, as a result shows, mandruka is used as training
Foster base carrier be both avoided that sand culture base particle be scattered everywhere, defect not easy to maintain, can guarantee that and filled with dioxygen water sterilization again
Point, wheat mildew phenomena is significantly reduced, effectively preventing and treating wheat seed goes mouldy.
The percentage that goes mouldy (%) of the different culture media carrier wheat of table 3
| Incubation time | Sand | Mandruka |
| 1d | 0 | 0 |
| 2d | 1.6 | 0 |
| 3d | 3.4 | 0.9 |
| 4d | 5.0 | 1.5 |
| 5d | 8.6 | 2.2 |
, but those skilled in the art once know basic creation although preferred embodiments of the present invention have been described
Property concept, then can make other change and modification to these embodiments.So, appended claims are intended to be construed to include excellent
Select embodiment and fall into having altered and changing for the scope of the invention.
Obviously, those skilled in the art can carry out the essence of various changes and modification without departing from the present invention to the present invention
God and scope.So, if these modifications and variations of the present invention belong to the scope of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to comprising including these changes and modification.
Claims (6)
1. a kind of wheat seedling cultural method, it is characterised in that comprise the following steps：
S1, wheat vernalization：The wheat seed for filtering out health carries out vernalization culture, obtains germinated wheat kind；
S2, is put into the culture vessel after sterilization after mandruka is sterilized, using mandruka as medium carrier, to porous sea
Culture matrix is injected in silk floss, sponge base culture medium is obtained；
The structure of the mandruka is：Take block sponge to cut and dig out multiple seed channels, each seed channel is shaped form
Shape, and the outlet of the seed channel is located at the upper surface of block sponge, and the bottom of seed channel is located inside block sponge；
S3, seedling culture：Germinated wheat kind is put into seed channel, then the mandruka for filling germinated wheat kind is placed in
Full exposure culture in constant incubator, until wheat seedling grows up to；
Wherein, daily supplementing culture medium matter extremely submerges mandruka upper surface during full exposure culture.
2. wheat seedling cultural method according to claim 1, it is characterised in that be the step of the vernalization culture：
(1) hydrogen peroxide dipping that wheat seed is placed in into volume fraction 1% is sterilized, and soak time is 30-60min, is sterilized
Wheat Species；
(2) sterilization Wheat Species are soaked in germination fluid, until wheat germination, obtains germinated wheat kind；
The germination fluid soaks juice according to 1 by the hydrogen peroxide and kuh-seng of volume fraction 1%:2 volume ratio is mixed；Wherein,
Kuh-seng leaching juice be by fresh kuh-seng boil, filter after collect obtained filtrate.
3. wheat seedling cultural method according to claim 2, it is characterised in that the preparation method of 1L kuh-sengs leaching juice is：
The fresh kuh-seng for taking 100g clean, is cut into the sophora film of 0.5-1cm thickness, is then placed in water sophora film and boils 1.5-2h,
Filtering, collects and considers liquid, finally supply 1L with sterilized water, obtains kuh-seng leaching juice.
4. wheat seedling cultural method according to claim 1, it is characterised in that seed channel in the mandruka
Curve shape is S-shaped shape or is 90 degree of arc shape.
5. wheat seedling cultural method according to claim 1, it is characterised in that the culture matrix is by following concentration
Component is made：0.5g/L KNO3, 0.8g/L Ca (NO3)2, 0.2g/L NH4H2PO4, 0.10g/L MgSO4、0.10g/L
Gibberellin, solvent is running water.
6. wheat seedling cultural method according to claim 1, it is characterised in that mandruka and culture vessel are used
The hydrogen peroxide dipping sterilization of volume fraction 1%, soak time is 10-20min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107439215A (en) * | 2017-09-19 | 2017-12-08 | 孙立成 | A kind of implantation methods of zinc-rich capsicum |
| CN112106477A (en) * | 2020-09-18 | 2020-12-22 | 扬州大学 | Method for determining influence of biogas slurry on wheat germination |
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| CN105706871A (en) * | 2016-01-28 | 2016-06-29 | 季稚寒 | Planting spongy substrate containing biomass activated carbon and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202819193U (en) * | 2012-07-03 | 2013-03-27 | 北京北农绿亨科技发展有限公司 | Substrate sponge for growing seedlings |
| CN103329789A (en) * | 2013-07-17 | 2013-10-02 | 武汉友芹种苗技术有限公司 | Soilless culture seedling-growing method for cress |
| CN105075839A (en) * | 2015-08-26 | 2015-11-25 | 青岛市农业科学研究院 | Water planting apparatus and water planning method for large-scale wheat drought resistance simulation |
| CN105494062A (en) * | 2015-12-22 | 2016-04-20 | 山东农业大学 | Liquid culture method of wheat seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107439215A (en) * | 2017-09-19 | 2017-12-08 | 孙立成 | A kind of implantation methods of zinc-rich capsicum |
| CN112106477A (en) * | 2020-09-18 | 2020-12-22 | 扬州大学 | Method for determining influence of biogas slurry on wheat germination |
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| Publication number | Publication date |
|---|---|
| CN107114225B (en) | 2019-11-12 |
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