CN105053519A - Method for increasing phaffia rhodozyma astaxanthin - Google Patents
Method for increasing phaffia rhodozyma astaxanthin Download PDFInfo
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- CN105053519A CN105053519A CN201510476474.8A CN201510476474A CN105053519A CN 105053519 A CN105053519 A CN 105053519A CN 201510476474 A CN201510476474 A CN 201510476474A CN 105053519 A CN105053519 A CN 105053519A
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- astaxanthin
- phaffia rhodozyma
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Abstract
The invention discloses a method for increasing phaffia rhodozyma astaxanthin, which comprises the following steps: adjusting pH value of a phaffia rhodozyma broth to 9.0-11.5, controlling the temperature at 35-60 DEG C, stirring for 80-150r/min, breaking wall for 1.0-5.0; neutralizing the pH value to 6.0-7.5, mixing with water having 0.5-1 times of volume, centrifuging and concentrating to obtain the concentrated wall breaking phaffia rhodozyma astaxanthin slurry; then uniformly mixing with a proper amount of complex formulation anti-oxidant and performing spray drying, wherein the air intake temperature is 170-185 DEG C, air-out temperature is 70-85 DEG C, and obtaining the astaxanthin dry powder, wherein the moisture of the dry powder is less than 8%, and spray loss amount is less than 4.0%. According to the invention, the method is simple and practical, total pigment can be increased by 10-12%, wall breaking time is short, cost is low, and the method is suitable for industrial production, shelf time of the produced astaxanthin dry powder is longer, taste is fragrant and unique, the phaffia rhodozyma astaxanthin has good palatability, and the dry powder can be widely used in animal feed industry.
Description
Technical field
The present invention relates to a kind of method improving red Phaffia Rhodozyma astaxanthin.
Background technology
Unique one that red phaffia rhodozyma (Phaffiarhodozyma) is mycota, Eumycota, Acarasiales subphylum, Cryptococcaceae, phaffia rhodozyma belong to.Obligate aerobic bacterium, its modes of reproduction is asexual budding.This bacterium fermentation energy produces 10 multiple types carrotene, and mainly contain astaxanthin, beta carotene, gamma carotene etc., wherein astaxanthin accounts for 60% ~ 85%.Cell produces carotenoid needs a large amount of oxygen.It can utilize several kinds of carbon source to ferment, and dextrose plus saccharose is optimum carbon source, is most suitable for growth temperature 20 ~ 28 DEG C, optimal pH 5.0 ~ 6.9, and red phaffia rhodozyma produces acid in aerobic fermentation process, release of carbon dioxide simultaneously synthesizing astaxanthin.
Phaffiafhodozyma, based on unicellular, can form pseudohypha sometimes.Bacterium colony produces the carotenoid such as astaxanthin because of thalline and takes on a red color, and carotenoid is distributed in cytolipin equably.Phaffiafhodozyma tool Crabtree effect (Crabtree).Phaffiafhodozyma growth temperature range is 4 ~ 27 DEG C, belongs to facultative addicted to cold low form microorganism.The most suitable growth pH is 6.0, and the optimal pH that pigment is formed is 5.0.
Current research showed that astaxanthin had extremely strong antioxidation, also has effects such as promoting the generation of body antibody, develop immunitypty and ultra-violet radiation resisting in recent years.Thus in food additives, aquaculture, cosmetics, health products and medical industry, more wide application prospect is had.
Natural astaxanthin (haematococcus pluvialis) achieves general safety certification (GRAS) at FDA, can be legal enter food and dietary supplements market.In August, 2000, FDA ratified the feed addictive of red phaffia rhodozyma as dog salmon.
Current astaxanthin technology is prepared production method and is first elected fermentable, but phaffia rhodozyma astaxanthin is generally far below Astaxanthin In Haematococcus Pluvialis content, especially astaxanthin is to sensitivities such as light, heat, oxygen, therefore except by improving except its fermentation level, but by chemical method mode skiving yeast cells wall thickness to reach the method for the content astaxanthin indirectly improving phaffia rhodozyma and to be applied in both at home and abroad that there is not been reported.
Summary of the invention
The object of the invention is to provide a kind of method improving red Phaffia Rhodozyma astaxanthin, on the one hand can than the total pigment of the more corresponding lifting 10 ~ 12% of original fermentation liquor total pigment content, the concentrated Fife yeast cream body of sporoderm-broken rate more than 80% can be obtained again simultaneously, broken time is short, cost is low, and suitability for industrialized is produced.
In order to reach above-mentioned purpose, solution of the present invention is:
Improve a method for red Phaffia Rhodozyma astaxanthin, the steps include:
The first step, red phaffia rhodozyma fermented liquid is adopted NaOH or potassium hydroxide solution modulation pH=9.0 ~ 11.5, temperature controls 35 ~ 60 DEG C, mixing speed 80 ~ 150r/min, broken time 1.0 ~ 5.0;
Second step, by the red phaffia rhodozyma fermented liquid neutralization after broken wall, to pH=6.0 ~ 7.5, and mixes with the water of 0.5 ~ 1 times of volume, through centrifugal concentrating, obtains the red Phaffia Rhodozyma slurry of concentrated broken wall; The broken wall obtained concentrate yeast cells content astaxanthin (unit) comparatively original fermentation liquor unit concentrate yeast cells content astaxanthin improve 10 ~ 12%;
3rd step, concentrated broken wall red Phaffia Rhodozyma slurry mixes rear spraying dry with appropriate compound antioxidant, EAT 170 ~ 185 DEG C, leaving air temp 70 ~ 85 DEG C, obtain astaxanthin dry powder, the moisture of astaxanthin dry powder is less than 8%, and wherein spraying dry astaxanthin loss amount is less than 4.0%.
Described 3rd step, compound antioxidant is vitamine C palmitate, one in L-AA sodium, sodium isoascorbate, natural VE, tea polyphenol-palmitate and BHA or compositely to be formed by two or more, compound antioxidant consumption account for by weight dry powder heavy 0.04 ~ 0.075%.
Described second step, neutralization adopt in citric acid and lactic acid any one.
After adopting such scheme, the invention has the beneficial effects as follows: simple and practical, the appropriateness that not only can realize total pigment improves, and easily realizes batch production, is applicable to industrial scale and produces; The astaxanthin dry powder storage rack time produced is longer, and taste raw meat is fragrant unique, and good palatability, power-product is widely used at animal feed industries.
Accompanying drawing explanation
Fig. 1 is process flow diagram of the present invention.
Detailed description of the invention
Coordinate Fig. 1 below, by embodiment, the present invention's related application in feed is described further.
Concrete grammar of the present invention is:
First, by red phaffia rhodozyma fermented liquid NaOH or potassium hydroxide solution modulation pH=9.0 ~ 11.5, temperature controls 35 ~ 60 DEG C, mixing speed 80 ~ 150r/min, broken time 1.0 ~ 5.0;
Again by the red phaffia rhodozyma fermented liquid neutralization after broken wall, adopt citric acid and lactic acid, adjust pH=6.0 ~ 7.5, and be watered, mix with the water of 0.5 ~ 1 times of volume, through centrifugal concentrating, obtain the red Phaffia Rhodozyma slurry of concentrated broken wall; The broken wall obtained concentrate yeast cells content astaxanthin (unit) comparatively original fermentation liquor unit concentrate yeast cells content astaxanthin improve 10 ~ 12%;
Then, concentrated broken wall red Phaffia Rhodozyma slurry mixes with appropriate compound antioxidant, compound antioxidant is vitamine C palmitate, one in L-AA sodium, sodium isoascorbate, natural VE, tea polyphenol-palmitate and BHA or compositely to be formed by two or more, compound antioxidant consumption account for by weight dry powder heavy 0.04 ~ 0.075%.After mixing, spraying dry, EAT 170 ~ 185 DEG C, leaving air temp 70 ~ 85 DEG C, obtains astaxanthin dry powder, and the moisture of astaxanthin dry powder is less than 8%, and wherein spraying dry astaxanthin loss amount is less than 4.0%.
The red phaffia rhodozyma dry powder of broken wall in embodiment is the product that Xiamen Huisheng Biological Co., Ltd. oneself produces, and its content astaxanthin is at 8000 ~ 10000mg/kg dry powder, and total pigment is at 14000 ~ 18000mg/kg dry powder.Dry powder moisture≤8%, crude protein >=20%.
Embodiment 1, adds the nutrition fortifier (amounting to astaxanthin 80 ~ 100mg/kg) containing 2.0 ~ 5.0% broken wall astaxanthin dry powder, feeds 6 weeks in grouper (porgy section class) bait, and individual growth contrasts 20 ~ 25% with control group.Feed in grouper nursery bait, survival rate and control group contrast and improve 18 ~ 22% respectively,
Embodiment 2, in shrimp seedling bait, add 1.8 ~ 3.0% broken wall astaxanthin dry powder (amounting to astaxanthin 60 ~ 80mg/kg), feed 15 days, the survival rate of shrimp seedling and control group contrast and are increased to 23 ~ 28%; Individual growth by more than 22.0%.
Embodiment 3. adds 2 ~ 4.0% broken wall astaxanthin dry powder in poultry basal feed, and feed after 4 ~ 6 weeks, skin, pin, beak present golden yellow, and these can both substantially increase the commodity value of birds, beasts and eggs, meat.In addition, astaxanthin promotes the growth of poultry as nutriment and improves laying rate.
Claims (3)
1. improve a method for red Phaffia Rhodozyma astaxanthin, it is characterized in that step is:
The first step, red phaffia rhodozyma fermented liquid is adopted NaOH or potassium hydroxide solution modulation pH=9.0 ~ 11.5, temperature controls 35 ~ 60 DEG C, mixing speed 80 ~ 150r/min, broken time 1.0 ~ 5.0;
Second step, being sent out by the red Phaffia Rhodozyma after broken wall is the neutralization of ferment liquid, to pH=6.0 ~ 7.5, and mixes with the water of 0.5 ~ 1 times of volume, through centrifugal concentrating, obtains the red Phaffia Rhodozyma slurry of concentrated broken wall;
3rd step, concentrated broken wall red Phaffia Rhodozyma slurry mixes rear spraying dry with compound antioxidant, EAT 170 ~ 185 DEG C, and leaving air temp 70 ~ 85 DEG C, obtains astaxanthin dry powder, and the moisture of astaxanthin dry powder is less than 8%.
2. a kind of method improving red Phaffia Rhodozyma astaxanthin as claimed in claim 1, it is characterized in that: described 3rd step, compound antioxidant is vitamine C palmitate, one in L-AA sodium, sodium isoascorbate, natural VE, tea polyphenol-palmitate and BHA or compositely to be formed by two or more, compound antioxidant consumption account for by weight dry powder heavy 0.04 ~ 0.075%.
3. a kind of method improving red Phaffia Rhodozyma astaxanthin as claimed in claim 1, is characterized in that: described second step, neutralizes any one in employing citric acid and lactic acid.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105434319A (en) * | 2015-12-07 | 2016-03-30 | 华南理工大学 | Cosmetics based on phaffia rhodozyma raw materials and preparation method thereof |
| CN105732452A (en) * | 2016-02-05 | 2016-07-06 | 厦门汇盛生物有限公司 | Method for extracting phaffia rhodozyma intracellular astaxanthin |
| CN107311313A (en) * | 2017-06-26 | 2017-11-03 | 安徽省黄淮兽药有限公司 | A kind of composite bacteria fermentation improver of water quality used for aquiculture |
| CN113265439A (en) * | 2021-06-08 | 2021-08-17 | 江苏海飞生物科技有限公司 | Method for preparing trans-astaxanthin |
| CN113331318A (en) * | 2021-06-07 | 2021-09-03 | 浙江博仕佳生物科技有限公司 | Preparation method of feed rich in yeast, astaxanthin, lactic acid and protease for shrimp and crab culture |
| CN114431333A (en) * | 2021-12-24 | 2022-05-06 | 青岛尚德生物技术有限公司 | Phaffia rhodozyma liquid composite preparation and preparation method and application thereof |
-
2015
- 2015-08-06 CN CN201510476474.8A patent/CN105053519A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105434319A (en) * | 2015-12-07 | 2016-03-30 | 华南理工大学 | Cosmetics based on phaffia rhodozyma raw materials and preparation method thereof |
| CN105434319B (en) * | 2015-12-07 | 2018-09-14 | 华南理工大学 | A kind of cosmetics and preparation method thereof based on phaffiafhodozyma raw material |
| CN105732452A (en) * | 2016-02-05 | 2016-07-06 | 厦门汇盛生物有限公司 | Method for extracting phaffia rhodozyma intracellular astaxanthin |
| CN107311313A (en) * | 2017-06-26 | 2017-11-03 | 安徽省黄淮兽药有限公司 | A kind of composite bacteria fermentation improver of water quality used for aquiculture |
| CN113331318A (en) * | 2021-06-07 | 2021-09-03 | 浙江博仕佳生物科技有限公司 | Preparation method of feed rich in yeast, astaxanthin, lactic acid and protease for shrimp and crab culture |
| CN113331318B (en) * | 2021-06-07 | 2024-02-20 | 浙江博仕佳生物科技有限公司 | Preparation method of feed for enriching yeast, astaxanthin, lactic acid and protease for shrimp and crab culture |
| CN113265439A (en) * | 2021-06-08 | 2021-08-17 | 江苏海飞生物科技有限公司 | Method for preparing trans-astaxanthin |
| CN114431333A (en) * | 2021-12-24 | 2022-05-06 | 青岛尚德生物技术有限公司 | Phaffia rhodozyma liquid composite preparation and preparation method and application thereof |
| CN114431333B (en) * | 2021-12-24 | 2024-04-02 | 青岛尚德生物技术有限公司 | Phaffia rhodozyma liquid compound preparation and preparation method and application thereof |
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Application publication date: 20151118 |