CN106701880A - Method for improving Phaffia rhodozyma strain high-yield astaxanthin - Google Patents
Method for improving Phaffia rhodozyma strain high-yield astaxanthin Download PDFInfo
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- CN106701880A CN106701880A CN201710030949.XA CN201710030949A CN106701880A CN 106701880 A CN106701880 A CN 106701880A CN 201710030949 A CN201710030949 A CN 201710030949A CN 106701880 A CN106701880 A CN 106701880A
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- astaxanthin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
Abstract
The invention provides a method for improving Phaffia rhodozyma strain high-yield astaxanthin. The method comprises the steps of multiplication culture and fermentation and separation, wherein a fermentation culture medium is prepared from, by weight, 75-85 parts of an agricultural and sideline product pretreatment solution, 20-30 parts of soybean meal, 8-14 parts of glucose, 4-8 parts of peptone, 4-8 parts of concentrated yeast extract powder, 0.2-0.4 part of CaCl2.2H2O, 1-4 parts of MgSO4.7H2O, 1-2 parts of KH2PO4, 1-5 parts of (NH4)2SO4, 1-3 parts of ethanol and 0.01-1 part of triethanolamine. According to the method, while the strain is stimulated to produce the astaxanthin, synthesis of the astaxanthin is promoted in the fermentation process, and therefore the astaxanthin yield is obviously increased, the fermentation cost is lowered, resources are fully utilized, triethanolamine promotes synthesis of the astaxanthin, and then the yield is obviously increased.
Description
Technical field
The invention belongs to fermentation arts, and in particular to the method for improving Phaffia rhodozyma strain high-yield astaxanthin.
Technical background
Astaxanthin (3,3 '-dihydroxy -4,4 ' diketo-β, β ' carrotene) is a kind of keto-acid carotenoid, naturally
Take on a red color.Astaxanthin is initially used as the feed addictive of aquatic products industry, and later pharmacology and Physiologic Studies show that astaxanthin has
Extremely strong function anti-oxidant, that free radical is quenched, and the generation of antibody can be promoted, strengthen the immunologic function of host, in food, doctor
The field such as medicine and cosmetics has broad application prospects, with economic worth very high.
Red phaffia rhodozyma（Phaffia rhodozyma）It is the only naturally production bacterial strain for producing astaxanthin, its trans shrimp
Blue or green element obtained FDA approvals in 2000, for food additives.It is mycota, Eumycota, Deuteromycotina, hidden ball ferment
Female section, red phaffia rhodozyma category unique kind.Used as the production bacterial strain of astaxanthin, red phaffia rhodozyma has many advantages：It is available
Various sugar carry out quick heterotrophism metabolism, and incubation time is short, are not required to illumination and can realize High Density Cultivation etc..Such as prior art Shen
Please number be the Chinese invention patent of CN103614444A, the invention belongs to biological technical field, there is provided one kind is using flammable
Astaxanthin contains in concentration of alcohol in the red phaffia rhodozyma zymotic fluid of gas sensor constant control, and then the red phaffia rhodozyma cell of raising
The method of amount, the method utilizes combustible gas sensor, and ethanol stream dosage, successfully constant control are controlled by switching peristaltic pump
Arbitrary value of the concentration of alcohol in red phaffia rhodozyma zymotic fluid in the range of 1 ~ 20g/L is made.In the red phaffia rhodozyma of constant control
In the case that concentration of alcohol is 10g/L in zymotic fluid, then by the culture of 1 day, the astaxanthin in red phaffia rhodozyma dry cell weight
Content can reach 145.7 μ g/g, comparison impinge upon do not flow plus ethanol under conditions of improve 140.4%, so as to reach red method
The purpose of husband's yeast high-yield astaxanthin, with important researching value and being widely applied prospect.The patent of invention is for equipment
It is very high with precise requirements, and inapplicable industrial production, such as Chinese invention of prior art Application No. CN105053519A
A kind of patent, method for improving red Phaffia Rhodozyma astaxanthin of the disclosure of the invention, first modulates pH=by red phaffia rhodozyma fermented liquid
9.0~11.5,35~60 DEG C are controlled, stir 80~150r/min, broken time 1.0~5.0；PH=6.0~7.5 are neutralized to again,
And mix with the water of 0.5~1 times of volume, by centrifugal concentrating, obtain concentrating the red Phaffia Rhodozyma slurry of broken wall；Then, with it is suitable
It is spray-dried after amount compound antioxidant is well mixed, 170~185 DEG C of EAT, 70~85 DEG C of leaving air temp obtains shrimp blue or green
Plain dry powder, dry powder moisture is less than 8%, and spray loss amount is less than 4.0%.The invention is simple and practical, can not only lift 10~12%
Total pigment, and broken time is short, low cost, suitable industrialized production, the astaxanthin dry powder storage rack time of production is longer, taste
Road raw meat is fragrant unique, and good palatability, power-product is widely used in animal feed industries.The prior art meets low cost, fits
Suitable industrialized production, but the yield of its astaxanthin need to be improved.
The content of the invention
The present invention provides a kind of astaxanthin yield raising Phaffia rhodozyma strain high yield high to solve above-mentioned technical problem
The method of astaxanthin.
The present invention is for the solution scheme taken of above-mentioned technical problem：Improve Phaffia rhodozyma strain high-yield astaxanthin
Method, including following steps：
Phaffia rhodozyma strain（Phaffia rhodozyma）CZ10 was preserved in positioned at Beijing Chaoyang on July 25th, 2012
In China Committee for Culture Collection of Microorganisms's common micro-organisms of the Institute of Microorganism, Academia Sinica of area North Star West Road 1
The heart, preserving number is CGMCC No.6355.
1）Activation Phaffia rhodozyma strain is inoculated in middle Shake flask medium at 20 ~ 27 DEG C for 5 ~ 12% by inoculum concentration, it is logical
Tolerance is 1.0 ~ 1.2vvm, stirs 100~200r/min, 20 ~ 35h of Amplification Culture；
2）Will be enlarged by the Phaffia rhodozyma strain after culture and be seeded to the fermentation tank equipped with fermentation medium by 6 ~ 10% inoculum concentration
In, ammonium sulfate is added as nitrogen source, make C/N ratios be 2；
3）The carrier of oxygen is added in fermentation tank, by volume, the addition of the carrier of oxygen is the 0.5 of fermentation medium percent by volume
~ 5%, 20 ~ 27 DEG C of cultivation temperature, the r/min of mixing speed 500 ~ 600, the vvm of throughput 1.2 ~ 1.5；
4）Fermentation process adds nutrient solution, while adding astaxanthin precursor substance every 12 ~ 24 h, 30 L is added every time, adds 2 ~ 5
Continue 20 ~ 30 h that ferment after secondary；
5）By 2 ~ 6 collects thallines of zymotic fluid centrifuge washing, thalline is suspended in 50 ~ 60 DEG C of dimethyl sulfoxide (DMSO)s, vibrated, acetone extraction
After taking, after vacuum concentration, dissolved again with dichloromethane, prepare the isolated astaxanthin component of HPLC chromatogram, be concentrated in vacuo
To astaxanthin dry powder；
Wherein, fermentation medium is made up of following composition and composition by weight：75 ~ 85 parts of agricultural byproducts pretreatment fluid, dregs of beans 20 ~ 30
Part, 8 ~ 14 parts of glucose, 4 ~ 8 parts of peptone, 4 ~ 8 parts of yeast extract powder of concentration, CaCl22H2O0.2 ~ 0.4 part, MgSO4
7H2O1 ~ 4 part, KH2PO41 ~ 2 part, (NH4) 2SO41 ~ 5 part, 1 ~ 3 part of ethanol, 0.01 ~ 1 part of triethanolamine, fermentation medium pH
Be worth is 5.5 ~ 6.5, hence it is evident that improve astaxanthin yield, while reducing fermentation costs, resource is fully used, wherein three ethanol
Amine promotes the synthesis of astaxanthin, so as to improve yield.Wherein Shake flask medium is made up of following composition and percentage：1 ~ 5% albumen
Peptone, 1 ~ 5% beef leaching thing, 4 ~ 10%NaCl, 0.01 ~ 0.11%MnSO4•H2O, 5 ~ 10% agar, 0.01 ~ 0.1% isooctanol and remaining
The sterilized water of amount, makes mycelial growth good, and bacterium colony is clearly demarcated, and wherein isooctanol stimulates bacterial strain to produce astaxanthin, so as to improve fermentation
During astaxanthin yield.The nutrient solution for adding during the fermentation is made up of following composition and percentage：1~4%(NH4)2·
The sterilized water of SO4,0.1 ~ 1% cellobiose, 0.1 ~ 1% KH2PO4,0.1 ~ 1% MnSO4,0.01 ~ 0.1% s-triazine and surplus,
Consumption and the shortage of carbon source in fermentation process are avoided, it is fully fermented, wherein s-triazine promotes fermentation abundant, so as to improve shrimp
Blue or green element yield, meanwhile, shorten its fermentation period.The carrier of oxygen added in fermentation process is n-dodecane and perfluocarbon；It is compound
Ratio is 1 ~ 2：1 ~ 2, promote oxygen transmission, dissolved oxygen amount in zymotic fluid is improved, so as to promote chemical activators, improve astaxanthin and produce
Amount, astaxanthin precursor substance described in astaxanthin precursor substance is tomato juice, beta carotene and amide blend, and compositely proportional is
5:4:1；The synthesis of astaxanthin can be promoted；Agricultural byproducts include blackstrap or brewer's yeast or microcrystalline cellulose or long-grained nonglutinous rice or corn
Core.
Compared with prior art, advantage for present invention is：While the present invention stimulates bacterial strain high-yield astaxanthin,
Promote the synthesis of astaxanthin simultaneously during the fermentation, so as to significantly improve astaxanthin yield, reduce fermentation costs, obtain resource
To making full use of, wherein triethanolamine promotes the synthesis of astaxanthin, so as to significantly improve yield.
Specific embodiment
It is described in further detail with reference to embodiments
Embodiment 1：
The method that Phaffia rhodozyma strain produces astaxanthin is improved, including following steps：
Phaffia rhodozyma strain（Phaffia rhodozyma）CZ10 was preserved in positioned at Beijing Chaoyang on July 25th, 2012
In China Committee for Culture Collection of Microorganisms's common micro-organisms of the Institute of Microorganism, Academia Sinica of area North Star West Road 1
The heart, preserving number is CGMCC No.6355.
1）Activation Phaffia rhodozyma strain is inoculated in middle Shake flask medium at 20 ~ 27 DEG C for 5 ~ 12% by inoculum concentration, it is logical
Tolerance is 1.0 ~ 1.2vvm, stirs 100~200r/min, 20 ~ 35h of Amplification Culture；
2）Will be enlarged by the Phaffia rhodozyma strain after culture and be seeded to the fermentation tank equipped with fermentation medium by 6 ~ 10% inoculum concentration
In, ammonium sulfate is added as nitrogen source, make C/N ratios be 2；
3）The carrier of oxygen is added in fermentation tank, by volume, the addition of the carrier of oxygen is the 0.5 of fermentation medium percent by volume
~ 5%, 20 ~ 27 DEG C of cultivation temperature, the r/min of mixing speed 500 ~ 600, the vvm of throughput 1.2 ~ 1.5；
4）Fermentation process adds nutrient solution, while adding astaxanthin precursor substance every 12 ~ 24 h, 30 L is added every time, adds 2 ~ 5
Continue 20 ~ 30 h that ferment after secondary；
5）By 2 ~ 6 collects thallines of zymotic fluid centrifuge washing, thalline is suspended in 50 ~ 60 DEG C of dimethyl sulfoxide (DMSO)s, vibrated, acetone extraction
After taking, after vacuum concentration, dissolved again with dichloromethane, prepare the isolated astaxanthin component of HPLC chromatogram, be concentrated in vacuo
To astaxanthin dry powder；
Wherein, fermentation medium is made up of following composition and composition by weight：75 ~ 85 parts of agricultural byproducts pretreatment fluid, dregs of beans 20 ~ 30
Part, 8 ~ 14 parts of glucose, 4 ~ 8 parts of peptone, 4 ~ 8 parts of yeast extract powder of concentration, CaCl2·2H2O0.2 ~ 0.4 part, MgSO4·
7H2O1 ~ 4 part, KH2PO41 ~ 2 part, (NH4)2SO41 ~ 5 part, 1 ~ 3 part of ethanol, 0.01 ~ 1 part of triethanolamine, fermentation medium pH value
It is 5.5 ~ 6.5, preferred ferment culture medium is made up of following composition and composition by weight：79 parts of agricultural byproducts pretreatment fluid, dregs of beans 26
Part, 13 parts of glucose, 6 parts of peptone, 6 parts of yeast extract powder of concentration, CaCl2·2H20.3 part of O, MgSO4·7H2O3 parts,
KH2PO41 part, (NH4)2SO43 parts, 1 part of ethanol, 0.01 part of triethanolamine, because the mechanism of action is still not clear, or because of synergy
Its astaxanthin yield is significantly improved, while reducing fermentation costs, resource is fully used, wherein triethanolamine promotes shrimp blue or green
The synthesis of element, so as to improve yield.Wherein Shake flask medium is made up of following composition and percentage：1 ~ 5% peptone, 1 ~ 5% N
Meat leaching thing, 4 ~ 10%NaCl, 0.01 ~ 0.11%MnSO4•H2O, 5 ~ 10% agar, 0.01 ~ 0.1% isooctanol and surplus it is aseptic
Water, it is preferred that Shake flask medium is made up of following composition and percentage：5% peptone, 5% beef leaching thing, 6%NaCl,
0.01%MnSO4•H2The sterilized water of O, 8% agar, 0.01% isooctanol and surplus；Make mycelial growth good, bacterium colony is clearly demarcated, wherein different
Octanol stimulates bacterial strain to produce astaxanthin, so as to improve astaxanthin yield in fermentation process.The nutrient solution for adding during the fermentation
It is made up of following composition and percentage：1~4%(NH4)2·SO4, 0.1 ~ 1% cellobiose, 0.1 ~ 1% KH2PO4、0.1~1%
MnSO4, 0.01 ~ 0.1% s-triazine and surplus sterilized water, it is preferred that the nutrient solution added in fermentation process by following composition and
Percentage is constituted：1%(NH4)2·SO4, 0.1% cellobiose, 0.3% KH2PO4、0.15% MnSO4, 0.01% s-triazine and surplus
Sterilized water, it is to avoid the consumption of carbon source and shortage in fermentation process, it is fully fermented, wherein s-triazine promotes fermentation abundant,
So as to improve astaxanthin yield, meanwhile, shorten its fermentation period.The carrier of oxygen added in fermentation process is n-dodecane and perfluor
Change carbon；Compositely proportional is 1 ~ 2：1 ~ 2, it is preferred that compositely proportional is 2：1, hence it is evident that promote oxygen transmission, improve dissolved oxygen in zymotic fluid
Amount, so as to promote chemical activators, hence it is evident that improve astaxanthin yield, astaxanthin precursor substance described in astaxanthin precursor substance for kind
Tomato juice, bata-carotene and amide blend, compositely proportional are 5：4:1；The synthesis of astaxanthin can be promoted；Agricultural byproducts include useless sugar
Honey or schlempe or microcrystalline cellulose or long-grained nonglutinous rice or corncob, reduce cost.
Embodiment 2：
Shake flask medium：5% peptone, 5% beef leaching thing, 6%NaCl, 0.01%MnSO4•H2It is O, 8% agar, 0.01% different pungent
The sterilized water of alcohol and surplus；79 parts of agricultural byproducts pretreatment fluid, 26 parts of dregs of beans, 13 parts of glucose, 6 parts of peptone, concentration yeast
Leach 6 parts of powder, CaCl2·2H20.3 part of O, MgSO4·7H2O3 parts, KH2PO41 part, (NH4)2SO43 parts, 1 part of ethanol, three second
0.01 part of hydramine；
Fermentation medium：79 parts of agricultural byproducts pretreatment fluid, 26 parts of dregs of beans, 13 parts of glucose, 6 parts of peptone, concentration yeast leaching
Go out 6 parts of powder, CaCl2·2H20.3 part of O, MgSO4·7H2O3 parts, KH2PO41 part, (NH4)2SO43 parts, 1 part of ethanol, three ethanol
0.01 part of amine；
Nutrient solution：：1%(NH4)2·SO4, 0.1% cellobiose, 0.3% KH2PO4、0.15% MnSO4, 0.01% s-triazine and remaining
The sterilized water of amount.
The good strain of activation culture is inoculated into Shake flask medium with 10% inoculum concentration, 21 DEG C, 170 rpm cultivate 18 h；
Finally it is inoculated with shaking flask, 6% inoculum concentration, 21 DEG C, 180 rpm cultures, 20 h；After corncob is crushed, with 4% sodium hydroxide solution
Treatment overnight, after filtering, is washed with water to neutrality, drying for standby.The sodium citrate buffer solution of 0.05 m of pH4.8 is subsequently adding,
115 DEG C of 20 min of sterilizing, add ammonium sulfate as nitrogen source, make C/N ratios be 2, are squeezed into culture medium with 700 L liquid amounts after mixing
In 1000 L fermentation tanks, inoculum concentration 6%, the carrier of oxygen is n-dodecane perfluocarbon, compositely proportional 2：1, the vvm of throughput 1.2, stir
Mix the r/min of speed 500,21 DEG C of fermentation temperature, pH is adjusted to 5.5 ~ 6.5, every 12 h addition one time of nutrition liquid adjust humidity to
30 ~ 40%, while every 24 h streams plus astaxanthin precursor substance tomato juice, bata-carotene and acid amides, being combined than row 5：4：1, every time
Plus 30 L, plus after 4 times, stop stream plus, continue 20 h that ferment.After fermentation ends, astaxanthin is extracted, astaxanthin is obtained after isolating and purifying
Yield is 81.56mg/L, refines total recovery and reaches 94.33%.
Although the present invention is disclosed as above with embodiment, it is not limited to protection scope of the present invention, any ripe
Those skilled in the art is known, in the change and retouching done without departing from the spirit and scope of the invention, this hair all should be belonged to
The protection domain of bright appended claims.
Claims (7)
1. the method for improving Phaffia rhodozyma strain high-yield astaxanthin, it is characterised in that following steps：
1）Activation Phaffia rhodozyma strain is inoculated in Shake flask medium at 20 ~ 27 DEG C for 5 ~ 12% by inoculum concentration, throughput is
1.0 ~ 1.2vvm, stirs 100~200r/min, 20 ~ 35h of Amplification Culture；
2）Will be enlarged by the Phaffia rhodozyma strain after culture to be seeded in fermentation tank by 6 ~ 10% inoculum concentration, add ammonium sulfate to make
It is nitrogen source, makes C/N ratios be 2；
3）The carrier of oxygen is added in fermentation tank, by volume, the addition of the carrier of oxygen is the 0.5 of fermentation medium percent by volume
~ 5%, 20 ~ 27 DEG C of cultivation temperature, the r/min of mixing speed 500 ~ 600, the vvm of throughput 1.2 ~ 1.5；
4）Fermentation process adds nutrient solution, while adding astaxanthin precursor substance every 12 ~ 24 h, 30 L is added every time, adds 2 ~ 5
Continue 20 ~ 30 h that ferment after secondary；
5）By 2 ~ 6 collects thallines of zymotic fluid centrifuge washing, thalline is suspended in 50 ~ 60 DEG C of dimethyl sulfoxide (DMSO)s, vibrated, acetone extraction
After taking, after vacuum concentration, dissolved again with dichloromethane, prepare the isolated astaxanthin component of HPLC chromatogram, be concentrated in vacuo
To astaxanthin dry powder；
Wherein, the fermentation medium is made up of following composition and composition by weight：75 ~ 85 parts of agricultural byproducts pretreatment fluid, dregs of beans
20 ~ 30 parts, 8 ~ 14 parts of glucose, 4 ~ 8 parts of peptone, concentration 4 ~ 8 parts of yeast extract powder, CaCl2·2H2O0.2 ~ 0.4 part,
MgSO4·7H2O1 ~ 4 part, KH2PO41 ~ 2 part, (NH4)2SO41 ~ 5 part, 1 ~ 3 part of ethanol, 0.01 ~ 1 part of triethanolamine.
It is 2. according to claim 1 to improve the method that Phaffia rhodozyma strain produces astaxanthin, it is characterised in that：The shaking flask
Culture medium is made up of following composition and percentage：1 ~ 5% peptone, 1 ~ 5% beef leaching thing, 4 ~ 10%NaCl, 0.01 ~ 0.11%
MnSO4•H2The sterilized water of O, 5 ~ 10% agar, 0.01 ~ 0.1% isooctanol and surplus.
It is 3. according to claim 1 to improve the method that Phaffia rhodozyma strain produces astaxanthin, it is characterised in that：The nutrition
Liquid is made up of following composition and percentage：1 ~ 4% (NH4) 2SO4,0.1 ~ 1% cellobiose, 0.1 ~ 1% KH2PO4,0.1 ~ 1%
The sterilized water of MnSO4,0.01 ~ 0.1% s-triazine and surplus.
It is 4. according to claim 1 to improve the method that Phaffia rhodozyma strain produces astaxanthin, it is characterised in that：The oxygen is carried
Body is n-dodecane and perfluocarbon；Compositely proportional is 1 ~ 2：1~2.
It is 5. according to claim 1 to improve the method that Phaffia rhodozyma strain produces astaxanthin, it is characterised in that：The shrimp is blue or green
Plain precursor substance is tomato juice, beta carotene and amide blend, and compositely proportional is 5：4:1.
6. it is according to claim 1 improve Phaffia rhodozyma strain high-yield astaxanthin method, it is characterised in that：The agriculture
Byproduct includes blackstrap or schlempe or microcrystalline cellulose or long-grained nonglutinous rice or corncob.
7. it is according to claim 1 improve Phaffia rhodozyma strain high-yield astaxanthin method, it is characterised in that：The hair
Ferment Medium's PH Value is 5.5 ~ 6.5.
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Cited By (8)
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| CN108410939A (en) * | 2018-07-12 | 2018-08-17 | 烟台大学 | A method of improving Determination of Astaxanthin in Haematococcus Pluvialis content |
| CN108893517A (en) * | 2018-07-19 | 2018-11-27 | 威海利达生物科技有限公司 | A kind of fermentation medium and method of red phaffia rhodozyma fermenting and producing astaxanthin |
| CN108913746A (en) * | 2018-07-19 | 2018-11-30 | 威海利达生物科技有限公司 | By improving red phaffia rhodozyma biomass synthesizing astaxanthin and method for measuring |
| CN108931601A (en) * | 2018-08-14 | 2018-12-04 | 威海利达生物科技有限公司 | A method of efficiently preparing astaxanthin mark product |
| CN108977493A (en) * | 2018-08-17 | 2018-12-11 | 青岛中科潮生生物技术有限公司 | The method that astaxanthin is prepared using lignocellulosic |
| CN108998493A (en) * | 2018-06-25 | 2018-12-14 | 浙江皇冠科技有限公司 | A kind of formula technique of high-yield astaxanthin fermentation medium and application |
| CN112266945A (en) * | 2020-09-29 | 2021-01-26 | 自然资源部第三海洋研究所 | Method for extracting astaxanthin from phaffia rhodozyma |
| CN114561304A (en) * | 2022-01-20 | 2022-05-31 | 厦门昶科生物工程有限公司 | Fermentation process for improving astaxanthin production of phaffia rhodozyma strains |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108998493A (en) * | 2018-06-25 | 2018-12-14 | 浙江皇冠科技有限公司 | A kind of formula technique of high-yield astaxanthin fermentation medium and application |
| CN108998493B (en) * | 2018-06-25 | 2020-11-06 | 浙江皇冠科技有限公司 | Formula technology and application of fermentation medium for high-yield astaxanthin |
| CN108410939A (en) * | 2018-07-12 | 2018-08-17 | 烟台大学 | A method of improving Determination of Astaxanthin in Haematococcus Pluvialis content |
| CN108893517A (en) * | 2018-07-19 | 2018-11-27 | 威海利达生物科技有限公司 | A kind of fermentation medium and method of red phaffia rhodozyma fermenting and producing astaxanthin |
| CN108913746A (en) * | 2018-07-19 | 2018-11-30 | 威海利达生物科技有限公司 | By improving red phaffia rhodozyma biomass synthesizing astaxanthin and method for measuring |
| CN108931601A (en) * | 2018-08-14 | 2018-12-04 | 威海利达生物科技有限公司 | A method of efficiently preparing astaxanthin mark product |
| CN108977493A (en) * | 2018-08-17 | 2018-12-11 | 青岛中科潮生生物技术有限公司 | The method that astaxanthin is prepared using lignocellulosic |
| CN112266945A (en) * | 2020-09-29 | 2021-01-26 | 自然资源部第三海洋研究所 | Method for extracting astaxanthin from phaffia rhodozyma |
| CN114561304A (en) * | 2022-01-20 | 2022-05-31 | 厦门昶科生物工程有限公司 | Fermentation process for improving astaxanthin production of phaffia rhodozyma strains |
| CN114561304B (en) * | 2022-01-20 | 2024-01-30 | 厦门昶科生物工程有限公司 | Fermentation process for improving astaxanthin production by rhodozyma strain |
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