CN105053078B - Application of the lily blade leaching liquor in parasitics weeds are prevented and treated - Google Patents
Application of the lily blade leaching liquor in parasitics weeds are prevented and treated Download PDFInfo
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Abstract
It is used to prevent and treat melon broomrape Parasitic Weeds as new and effective plant source the invention discloses lily blade powder or a kind of new application of its leaching liquor, i.e. lily blade.Experiment is proved, four kinds of concentration water extracts (10mg/ml, 1mg/ml, 0.1mg/ml, 0.01mg/ml) of lily blade are respectively provided with stronger germination stimulation effect to Parasitic Weeds melon broomrape seed, wherein when diluted concentration is to 0.1mg/ml, it stimulates germination stimulation rate of the germination rate of melon broomrape seed with artificial synthesized analog GR24 to be sufficiently close to.In addition, measure is re-started to sealed storage lily blade material more than half a year, it was found that its germination stimulation ability only has 20% 30% loss, it is inferred that lily blade can folk prescription be used to prepare the parasitic melon broomrape weeds preparation of preventing and treating or constitute high-efficient compound preparation with other drugs.
Description
Technical field
The present invention relates to a kind of botanical herbicides, specially a kind of lily blade water extract is miscellaneous in preventing and treating broomrape parasitism
New application in terms of grass.
Background technology
Melon broomrape is holoparasite weeds, and the whole world is widely distributed and main parasitic is various melon, tomato, tobacco, to
On the roots of industrial crops such as day certain herbaceous plants with big flowers, cucurbit, carrot, cause a large amount of underproduction of local crops, brought to agricultural production with economy
Serious influence.Further, since broomrape seed quantity in stock in soil is big, the time-to-live is long, only by machinery, artificial, agriculture
The methods such as medicine is sprayed, the seed selection of anti-grass product kind are prevented and treated, and not only expend a large amount of manpower and materials, welding is also difficult to reach root
The effect removed.
It has now been found that many broomrapes are parasitic or non-parasitic plant can secrete the secondary metabolism for stimulating its seed to sprout
Material, if using this Germination stimulant induce broomrape seed in the presence of no host plant it is autonomous sprout but because
Host can not be found in time and normal sorption is parasitic so as to cause broomrape seed " Suicide sprouting ", and this one kind of can yet be regarded as effectively subtracts
The effective ways of few soil broomrape seed bank.And by the artificial synthesized analog such as GR6 for stimulating broomrape seed to sprout,
GR7 and GR24 etc., but be due to that synthetic material's matter cost is too high, now only rest on the laboratory research stage, it is impossible to enter to do business
Industryization is promoted and application.Therefore more efficient Plant source active substances are found and promote melon broomrape seed " Suicide sprouting ", are
The hot fields of the current preventing and treating Parasitic Weeds of research both at home and abroad.
So far, research and development obtain botanical herbicides product mainly act on common weeds such as Amaranthus retroflexus, barnyard grass and
Wild oat etc., and for preventing and treating the green herbicide achievement in research of the plants such as parasitics weeds such as broomrape or witchweed relatively
It is few, wherein artificial synthesized Parasitic Weeds sprout stimulant, because its production cost is higher, do not put into production application
In.And existing plant source preventing and treating Parasitic Weeds preparation such as faenum graecum soluble powder, on the one hand because its making formula is complicated
And preparation process is cumbersome, the raising of large-scale production cost is caused.On the other hand, because this formulation concentrations need to reach 100mg/
It stimulates Parasitic Weeds seed germination rate just close to 50% to more than ml, and artificial synthesized effective sprouting stimulant GR24 is extremely low
Concentration such as 1 μ g/m if can reach more than 80%, therefore using the less efficient of faenum graecum soluble powder preventing and treating Parasitic Weeds,
And input cost is higher.
Lily is the perennial bulb bearing plant of Liliaceae lilium, and it is as one of world-renowned flowering bulb, with very
High ornamental value.Meanwhile, lily is because rich in a variety of neutraceutical active ingredients, so as to be good strong with higher nutritive value
Complicated secondary metabolites contained by body invigorant, wherein lily is calmed the nerves for relieving cough and reducing sputum, hypnosis and oncotherapy has aobvious
The effect of work.But the ability whether the lily plant with so abundant active component has preventing and treating broomrape weeds is not appeared in the newspapers yet
Road, and the product of induction broomrape seed " Suicide sprouting " does not also occur using lily extract as plant source.Therefore exploitation with
Lily extract is the botanical herbicides of active component, there is huge research potential and wide market development prospect.
The content of the invention
In order to solve the above problems, the present invention provides a kind of lily blade powder or its leaching liquor is preventing and treating parasitics weeds
In application
First, the present invention provides the application of lily blade powder or its leaching liquor in parasitics weeds are prevented and treated, and it is will
Lily blade powder is extracted, and soil is carried out in soil before crop is sowed or when sowing, leaching liquor being sprayed or being sprayed on
Processing, stimulates the parasitics weed seed being detained in soil to carry out Suicide sprouting, so as to prevent and treat the parasitics weeds in farmland.
Wherein, the lily blade leaching liquor is that lily blooms the blade in later stage, is dried through cool place and uses water after crushing
Leaching liquor obtained by mechanical shaking extraction.
Preferably, the lily cultivar is new PE curriculum.
Wherein, the concentration of described lily blade leaching liquor is 0.01~10mg/ml.
Wherein, described parasitics weeds are Orobanche weeds, it is more preferred to melon broomrape.
Secondly, the present invention also provides a kind of parasitics weed germination inhibitor, and it contains active component, described activity into
It is divided into lily blade powder or its leaching liquor.
Wherein, then leaching liquor can be diluted to this hair by lily blade powder with the leaching liquor obtained by water mechanical shaking extraction
Bright required concentration.
Wherein, the inhibition of seed germination agent can suppress Orobanche weeds, preferably melon broomrape.
It is an object of the invention to a kind of new application of open lily blade water extract, i.e. lily blade is used as new height
Effect plant source is used to prevent and treat melon broomrape Parasitic Weeds.It is demonstrated experimentally that lily blade four kinds of concentration water extracts (10mg/ml,
1mg/ml, 0.1mg/ml, 0.01mg/ml) stronger germination stimulation effect is respectively provided with to Parasitic Weeds melon broomrape seed, wherein when
When diluted concentration is to 0.1mg/ml, it stimulates the germination stimulation of the germination rate of melon broomrape seed with artificial synthesized analog GR24
Rate is sufficiently close to.In addition, re-starting measure to sealed storage lily blade material more than half a year, its germination stimulation energy is found
Power only has 20%-30% loss, it is inferred that lily blade can folk prescription be used to preparing the parasitic melon broomrape weeds preparation of preventing and treating or
High-efficient compound preparation is constituted with other drugs.
Lily blade water extract of the present invention, specifically refers to new PE curriculum and blooms the blade material in later stage, through the moon
Airing it is dry and after crushing using the leaching liquor obtained by pure water mechanical shaking extraction.Thus the leaching liquor finally obtained can be used for stimulating parasitic
Weeds melon broomrape seed " Suicide sprouting ", therefore using this feature of new PE curriculum blade, can develop efficient, free of contamination
Green is agricultural to remove Parasitic Weeds preparation.
The beneficial effects of the invention are as follows：Bulbus Lilii leaf tablet preparation is dissolved in after water, can efficiently stimulate melon broomrape seed to sprout in advance,
Because seed fails to find host in time and carries out absorption life, so as to cause melon broomrape " commit suiside and sprout ", therefore it can be effectively used for
The biological control work of melon broomrape Parasitic Weeds, and the method is free from environmental pollution, while weeds will not also develop immunity to drugs
Feature, application prospect is very wide, with significant Social benefit and economic benefit.
Brief description of the drawings
Fig. 1 show influence of the different lily cultivar blade infusions to melon broomrape seed germination rate.
Fig. 2, which show storage time, stimulates new PE curriculum blade the influence of melon broomrape seed germination rate.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Test sample：Lily ball is purchased from Beijing lily company.
Laboratory apparatus：High speed disintegrator (FW135-177, Tianjin Stettlen Instrument Ltd.)；60 mesh (0.45mm) are sieved；
Supersonic oscillations instrument；Intelligent growth cabinet.
Extracting method：Harvesting new PE curriculum, oriental hybrid lily and Lilium Pollyanna Post flowering are the hundred of daughter bulb weightening period
Hing straps, are placed on shady place drying, then (FW135-177, Tianjin Stettlen instrument is limited using high speed disintegrator first
Company) crush and cross 60 mesh (0.45mm) sieve, collect lily blade powder in sealed plastic pipe, be placed in room temperature storage and treat
Survey.
Mother liquor is configured：New PE curriculum to be measured, oriental hybrid lily and Lilium Pollyanna blade powder 0.1g are weighed respectively in 1.5mL
In centrifuge tube, sterilized water 1mL is added, is extracted using supersonic oscillations and 6400rpm centrifuge 2min is used after 30min, will
Above-mentioned three kinds of lily blades filter respectively obtained by supernatant be set to stoste, i.e. new PE curriculum supernatant water extract (AELL),
Oriental hybrid lily supernatant water extract (AEOL) and Lilium Pollyanna supernatant water extract (AEAL), then by obtain three kinds of lily product
Plant stoste and dilute 10 times, 100 times, 1000 times, 10000 times, dilution (10mg/ml, 1mg/ of four kinds of various concentrations is obtained
Ml, 0.1mg/ml, 0.01mg/ml), each concentration sets 3 repetitions.
For examination weeds and medicine：Melon broomrape seed and witchweed lactone analogue (1 μ g/ml GR24) are provided by water and soil
Source keeps research center horse Yongqing professor to provide.
Embodiment 1
Test method：First in culture dish, respectively by four kinds of various concentrations water loggings of above three lily cultivar blade
The μ l of extract 30 are added on a diameter of 5mm circular glass fiber filter disc with melon broomrape seed respectively, and culture dish is close with sealed membrane
Envelope, is subsequently placed at culture in 25 DEG C of constant temperature dark culturing casees.Meanwhile, using 1 μ g/ml GR24 and distillation water process as positive and negative right
According to.The germination percentage of broomrape seed is observed and counted after one week under the microscope, seed is sprouted to break in the seed coat as standard so that sucker is prominent.
Result of the test：As shown in figure 1, the germination percentage of 1 μ g/ml GR24 processing melon broomrape seeds is more than 80%, and steam
Distilled water processing melon broomrape seed germination percentage be 0, illustrate melon broomrape seed must in the presence of Germination stimulant ability
Germination, while showing that melon broomrape seed vitality is stronger, can be used for germination test.
In addition, the non-stimulated melon broomrape seed of four kinds of concentration water extracts of oriental hybrid lily and Lilium Pollyanna sprouts ability,
And four kinds of concentration water extracts of new PE curriculum blade are respectively provided with very high germination stimulation effect to melon broomrape seed, and suitable
Concentration range (10mg/ml-0.1mg/ml) in the increase of leaching liquor extension rate, melon broomrape seed germination rate present by
Gradually elevated trend.Wherein when lily blade water extract concentration is 0.1mg/ml, it stimulates the germination rate of melon broomrape seed
Artificial synthesized analog GR24 germination stimulation rate is sufficiently close to, dilution lily blade water extract is continued thereafter with, when concentration is
During 0.01mg/ml, it stimulates melon broomrape seed germination rate to decline, therefore can be by adjusting new cone drums blade water extract
Concentration, reaches that efficient stimulation melon broomrape seed sprouts ability.
Embodiment 2
Test method：As shown in Fig. 2 weighing the sealed type storage lily blade powder to be measured of more than 1 year re-starts melon row
When seed sprouts measuring.Specific detection method is ibid.
Result of the test：The sealed storage lily blade material of more than 1 year is compared with fresh leaf material, in identical dilution
Under concentration, the former stimulates melon broomrape seed germination rate to be approximately less than the latter 20%-30%, i.e. sealed type storage stimulation in more than 1 year
The ability that melon broomrape is sprouted has 20%-30% loss.Therefore can be in a timing using lily blade powder as single preparations of ephedrine
The interior ability for more stably keeping preventing and treating Parasitic Weeds melon broomrape.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, some improvements and modifications can also be made, these improvements and modifications
Also it should be regarded as protection scope of the present invention.
Claims (5)
1. the application of new PE curriculum blade powder or its leaching liquor in parasitics weeds are prevented and treated, it is characterised in that by new iron
Big gun lily blade powder is extracted, before crop sowing or when sowing, and progress soil in soil is sprayed or be sprayed on by leaching liquor
Earth processing, stimulates the parasitics weed seed being detained in soil to carry out Suicide sprouting, so that the parasitics prevented and treated in farmland is miscellaneous
Grass.
2. application as claimed in claim 1, it is characterised in that the new PE curriculum blade leaching liquor is opened for new PE curriculum
The blade in later stage is spent, using the leaching liquor obtained by water mechanical shaking extraction after shady and cool dry and crush.
3. application as claimed in claim 1 or 2, it is characterised in that the concentration of described new PE curriculum blade leaching liquor is
0.01~10mg/ml。
4. application as claimed in claim 1 or 2, it is characterised in that described parasitics weeds are Orobanche weeds.
5. application as claimed in claim 4, it is characterised in that described parasitics weeds are melon broomrape.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510463678.8A CN105053078B (en) | 2015-07-31 | 2015-07-31 | Application of the lily blade leaching liquor in parasitics weeds are prevented and treated |
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| CN201510463678.8A CN105053078B (en) | 2015-07-31 | 2015-07-31 | Application of the lily blade leaching liquor in parasitics weeds are prevented and treated |
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| CN105053078B true CN105053078B (en) | 2017-10-27 |
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| CN106305784A (en) * | 2016-06-30 | 2017-01-11 | 黄正西 | Microbial preparation for removing edible sunflower orobanche |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP2348816A1 (en) * | 2008-11-18 | 2011-08-03 | State Of Israel, Ministry Of Agriculture And Rural Development, A.R.O. | Pest resistant plant |
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| CN102525851A (en) * | 2010-12-17 | 2012-07-04 | 强生消费者公司 | Compositions comprising lilium martagon extracts and uses thereof |
| CN102552084A (en) * | 2010-12-17 | 2012-07-11 | 强生消费者公司 | Compositions comprising lilium martagon extracts and uses thereof |
| CN103875721A (en) * | 2014-02-17 | 2014-06-25 | 烟台玉龙生物科技有限公司 | Plant source herbicide and preparation method thereof |
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