CN105028896A - Method for preparing sea-buckthorn biological fodder through compound microbial flora fermentation - Google Patents
Method for preparing sea-buckthorn biological fodder through compound microbial flora fermentation Download PDFInfo
- Publication number
- CN105028896A CN105028896A CN201510600355.9A CN201510600355A CN105028896A CN 105028896 A CN105028896 A CN 105028896A CN 201510600355 A CN201510600355 A CN 201510600355A CN 105028896 A CN105028896 A CN 105028896A
- Authority
- CN
- China
- Prior art keywords
- buckthorn
- sea
- biological feed
- pomace
- biological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a method for preparing sea-buckthorn biological fodder through compound microbial flora fermentation. According to the method, compound microbial flora is adopted, and the compound microbial flora is composed of 0.5-5% of bacillus subtilis and 1-8% of beer yeast. Multi-flora coordinated fermentation sea-buckthorn fruit residues are adopted, through the coordinated transformation of different microorganisms, the bioavailability of the fruit residues is improved, so that produced sea-buckthorn biological fodder is high in disgestibility, the coarse fiber content is reduced, the protein content is substantially raised, the number of probiotics is substantially increased, the method has the advantages of being complete in nutrition, green, safe, low in cost, high in economic benefit and the like, the growth speed of beasts and birds can be remarkably enhanced, the disease-suffering rate of the beasts and birds can be lowered, the feed conversion ratio in the beast and bird culturing process is reduced, the method is applicable to large-scale and industrialization production, and the wide application and popularization prospect is achieved.
Description
Technical field
The invention belongs to the preparing technical field of biological feedstuff, particularly a kind of complex microbial community fermentation is for the method for sea-buckthorn biological feed, is specifically related to be that primary raw material adopts compound microorganism ferments to prepare the method for sea-buckthorn biological feed with sea-buckthorn pomace.
Background technology
Sea-buckthorn, also sour willow, acid thorn, black thorn, vinegar thorn willow, arrow-leaved oleaster is, the Inner Mongol is feeling unhappy and ashamed, it is a kind of broad-spectrum wild plant Shu Hu Decadent scarabaeidae (Elaeagnaceae) Hippophne (HippophaerhamnoidesLinn.), China is distributed with 7 kinds, 7 subspecies, and there is 7 kind of 11 subspecies in the world.The world is distributed in the sub-south of this prestige born of the Atlantic coast and western European, until the Balkan Peninsula, Turkey, Iran, Afghanistan, Pakistan, Russia, north India.China is distributed in the provinces and regions such as the Inner Mongol, Hebei, Shanxi, Shaanxi, Gansu, Ningxia, Qinghai, Xinjiang, Sichuan, Yunnan, Guizhou, Tibet, Liaoning.Sea buckthorn fruit is good health food and medicine; Leaves of Hippophae L, can tea making or make feed also containing important active material; Bark, containing tannin, can obtain through refining tannin extract; Seed can process grease; Flower not only can do nectar source, also can lixiviate essence; Root has rhizobium, can improve soil, increases fertility.So sea-buckthorn is the important source material of the industry such as beverage, food, health products, medicine.
Because sea-buckthorn contains abundant nutritional labeling and various bioactivators, sea-buckthorn is constantly developed to various food, health products, cosmetics and medicine.Sand spine serial product continually develop production, make the leftover bits and pieces bulk depositions such as sea-buckthorn skin slag.Due to these leftover bits and pieces moisture higher (seabuckthorn fruit peel water content is about 50%), and acidity is large, is deposited in very easily to become sour rotten together, not only causes environmental pollution, wastes resource.Sea-buckthorn pomace is the accessory substance after fructus hippophae squeezing, containing abundant fat, vitamin, protein and other various active materials, is splendid high-quality feed resource.Research shows, sea-buckthorn pomace has facilitation in various degree to the growth of livestock and poultry and the raising of production performance, can improve efficiency of feed utilization and yolk carotene carotene content, and has the function reducing cholesterol, the growth of Promote immunity organ growth.
Although sea-buckthorn pomace is containing abundant nutritional labeling and active material, also have that content of cellulose is high, the shortcoming of low percentages of protein, be not too applicable to directly as animal and fowl fodder.Select based on bacillus, be aided with the complex microbial community that brewer's yeast is formed, bacillus has stronger protease, amylase and lipase active, also there is the enzyme of complex carbohydrates in degraded feed simultaneously, as pectase, dextranase, cellulase etc., these enzymes can destroy the cell membrane of plant feed cell, impel the nutriment of cell to discharge, and the ANFs can eliminated in feed, reduce the obstacle that ANFs utilizes animal digestion.This flora have breeding fast, vitality is strong, volume is large, take up space advantage, can suppress the growth and breeding of harmful bacteria.This research adopts effectively compound microbial inoculant integrated treatment sea-buckthorn pomace to be processed into biological feedstuff, this technology is while solution sea-buckthorn pomace environmental pollution problem, the stodgy compositions such as the cellulose that can effectively degrade in sea-buckthorn pomace, improve the protein content of sea-buckthorn pomace, increase the quantity of probio in sea-buckthorn pomace, significantly improve sea-buckthorn biological quality of the fodder, turn waste into wealth, the overall availability of raising sea buckthorn resources, the economic benefit of raising enterprise are had great importance.
The present invention adopts complex microbial community to carry out fermentation process to sea-buckthorn pomace, improve the Amino acid and protein content of sea-buckthorn pomace, improve the mouthfeel of sea-buckthorn feed, improve the quality of sea-buckthorn biological feed, there is small investment, the advantage such as simple to operate, fermentation condition is gentle, do not pollute, be convenient to manufacturer accept, effectively improve the utilization rate of sea buckthorn resources, improve the quality of sea-buckthorn feed, improve the economic benefit of enterprise, having low energy consumption, environmental protection, free of contamination feature, is a kind of production method of up-and-coming sea-buckthorn biological feed.
Summary of the invention
The technical barrier that quasi-solution of the present invention is determined is to provide the method for a kind of complex microbial community fermentation for sea-buckthorn biological feed, the method adopts the coordinative role of the different biology enzymes produced in compound microorganism ferments process to be used to the original composition of sea-buckthorn, reduce, decompose sea-buckthorn content of cellulose, the simultaneously growth of microorganism self significantly improves the protein content of sea-buckthorn feed, make the sea-buckthorn biological feed produced become a kind of protein content high, nutritious, containing the good hay varieties of various active material, palatability.
For solving above-mentioned technical barrier, the technical solution used in the present invention is as follows:
Complex microbial community fermentation is for a method for sea-buckthorn biological feed, and the method comprises the steps:
(1) complex microorganism seed good for preculture is prepared for subsequent use according to 0.5 ~ 5% bacillus subtilis and 1 ~ 8% brewer's yeast;
(2) sea-buckthorn pomace adds ammonium sulfate, the potassium dihydrogen phosphate of 0.2 ~ 1.0%, magnesium sulfate, the urea of 0.2 ~ 2.0%, the glucose of 1 ~ 5% of 0.2 ~ 1.0% of 1 ~ 4% according to formula, and adjustment water content is 40 ~ 70%, pH4 ~ 7;
(3) the pomace culture medium prepared is placed on sterilizing in koji tray, temperature 90 ~ 120 DEG C, time 30 ~ 120min;
(4) in the sea-buckthorn pomace culture medium that sterilizing is good, access complex microorganism seed, inoculum concentration is 2 ~ 6%, then 30 ± 5 DEG C of fermentations 36 ~ 72 hours;
(5) the sea-buckthorn biological feed drying fermented, be crushed to 40 ~ 1000 orders according to the purposes difference of biological feedstuff further, quantitative separating gets product.
In step (1), the complex microorganism seed proportioning that preculture is good is that 0.5 ~ 5% bacillus subtilis and 1 ~ 8% brewer's yeast mix.
In step (2), the formula of sea-buckthorn pomace culture medium is ammonium sulfate, the potassium dihydrogen phosphate of 0.2 ~ 1.0%, magnesium sulfate, the urea of 0.2 ~ 2.0%, the glucose of 1 ~ 5% of 0.2 ~ 1.0% of 1 ~ 4%, and adjustment water content is 40 ~ 70%, pH4 ~ 7.
In step (3), the sterilising conditions of pomace culture medium is temperature 90 ~ 120 DEG C, time 30 ~ 120min, naturally cools to about 30 DEG C after sterilizing terminates.
In step (4), the condition of culture of sea-buckthorn biological feed is the inoculum concentration of complex microorganism seed is 2 ~ 6%, and fermentation condition is temperature 30 ± 5 DEG C, 36 ~ 72 hours time.
In step (5), the aftertreatment technology of sea-buckthorn biological feed is for drying, and be crushed to 40 ~ 1000 orders according to the purposes difference of biological feedstuff, quantitative separating gets product.
Beneficial effect: advantage of the present invention is:
1. the present invention adopts many floras to coordinate fermentation sea-buckthorn pomace, transformed by the coordination of different microorganisms, improve the bioavailability of pomace, make the sea-buckthorn biological feed digestibility of production high, crude fiber content declines, protein content significantly raises, and probio quantity significantly improves, and has the advantages such as nutrition full price, green safety, cost are low, good palatability, economic benefit are high.
2. the sea-buckthorn biological feed that the present invention produces can significantly improve the speed of growth of livestock and poultry, reduces the rate of falling ill of livestock and poultry, reduces the feedstuff-meat ratio of livestock and poultry cultivation, is suitable for scale, industrialization is produced, have wide application prospect.
3. invention significantly improves the overall availability of sea buckthorn resources, solve the pollution problem of sea-buckthorn discarded object, technical advantage is obvious, has higher economic benefit and social benefit.
Accompanying drawing explanation
Fig. 1 is process chart of the present invention.
Detailed description of the invention
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1:
By complex microorganism seed good for preculture according to 1% bacillus subtilis and 3% brewer's yeast ratio to carry out premixed for subsequent use.In sea-buckthorn pomace, add ammonium sulfate, the potassium dihydrogen phosphate of 0.5%, magnesium sulfate, the urea of 0.4%, the glucose of 2% of 0.3% of 2% according to formula, adjustment water content is about about 55%, pH5, mixes.The sea-buckthorn pomace culture medium prepared is placed on sterilizing in koji tray, temperature 100 DEG C, and time 60min, the culture medium that sterilizing completes naturally cools to about 30 DEG C.In the sea-buckthorn pomace culture medium that sterilizing is good, access complex microorganism seed, inoculum concentration is 4%, then 30 ± 5 DEG C of fermentations 60 hours; The sea-buckthorn biological feed fermented is 55 ~ 60 DEG C of oven dry, and be crushed to 80 orders, quantitative separating gets product.Product protein content is 40.42%.
Embodiment 2:
By complex microorganism seed good for preculture according to 0.8% bacillus subtilis and 4% brewer's yeast ratio to carry out premixed for subsequent use.In sea-buckthorn pomace, add ammonium sulfate, the potassium dihydrogen phosphate of 0.8%, magnesium sulfate, the urea of 0.6%, the glucose of 2% of 0.5% of 1.5% according to formula, adjustment water content is about about 58%, pH5.2, mixes.The sea-buckthorn pomace culture medium prepared is placed on sterilizing in koji tray, temperature 98 DEG C, and time 90min, the culture medium that sterilizing completes naturally cools to about 30 DEG C.In the sea-buckthorn pomace culture medium that sterilizing is good, access complex microorganism seed, inoculum concentration is 5%, then 30 ± 5 DEG C of fermentations 48 hours; The sea-buckthorn biological feed fermented is 55 ~ 60 DEG C of oven dry, and be crushed to 60 orders, quantitative separating gets product.Product protein content is 40.16%.
Embodiment 3:
By complex microorganism seed good for preculture according to 1% bacillus subtilis and 4% brewer's yeast ratio to carry out premixed for subsequent use.In sea-buckthorn pomace, add ammonium sulfate, the potassium dihydrogen phosphate of 0.4%, magnesium sulfate, the urea of 0.5%, the glucose of 1.5% of 0.2% of 2% according to formula, adjustment water content is about about 60%, pH6, mixes.The sea-buckthorn pomace culture medium prepared is placed on sterilizing in koji tray, temperature 100 DEG C, and time 60min, the culture medium that sterilizing completes naturally cools to about 30 DEG C.In the sea-buckthorn pomace culture medium that sterilizing is good, access complex microorganism seed, inoculum concentration is 5%, then 30 ± 5 DEG C of fermentations 48 hours; The sea-buckthorn biological feed fermented is 55 ~ 60 DEG C of oven dry, and be crushed to 100 orders, quantitative separating gets product.Product protein content is 41.08%.
Claims (6)
1. complex microbial community fermentation is for a method for sea-buckthorn biological feed, it is characterized in that, the method comprises the steps:
Complex microorganism seed good for preculture is prepared for subsequent use according to 0.5 ~ 5% bacillus subtilis and 1 ~ 8% brewer's yeast;
Sea-buckthorn pomace adds ammonium sulfate, the potassium dihydrogen phosphate of 0.2 ~ 1.0%, magnesium sulfate, the urea of 0.2 ~ 2.0%, the glucose of 1 ~ 5% of 0.2 ~ 1.0% of 1 ~ 4% according to formula, and adjustment water content is 40 ~ 70%, pH4 ~ 7;
The pomace culture medium prepared is placed on sterilizing in koji tray, temperature 90 ~ 120 DEG C, time 30 ~ 120min;
In the sea-buckthorn pomace culture medium that sterilizing is good, access complex microorganism seed, inoculum concentration is 2 ~ 6%, then 30 ± 5 DEG C of fermentations 36 ~ 72 hours;
The sea-buckthorn biological feed drying fermented, be crushed to 40 ~ 1000 orders according to the purposes difference of biological feedstuff further, quantitative separating gets product.
2. complex microbial community fermentation according to claim 1 is for the method for sea-buckthorn biological feed, it is characterized in that: in step (1), and the complex microorganism seed proportioning that preculture is good is 0.5 ~ 5% bacillus subtilis and 1 ~ 8% brewer's yeast.
3. complex microbial community fermentation according to claim 1 is for the method for sea-buckthorn biological feed, it is characterized in that: in step (2), the formula of sea-buckthorn pomace culture medium is ammonium sulfate, the potassium dihydrogen phosphate of 0.2 ~ 1.0%, magnesium sulfate, the urea of 0.2 ~ 2.0%, the glucose of 1 ~ 5% of 0.2 ~ 1.0% of 1 ~ 4%, adjustment water content is 40 ~ 70%, pH4 ~ 7.
4. complex microbial community fermentation according to claim 1 is for the method for sea-buckthorn biological feed, it is characterized in that: in step (3), and the sterilising conditions of pomace culture medium is temperature 90 ~ 120 DEG C, time 30 ~ 120min.
5. complex microbial community fermentation according to claim 1 is for the method for sea-buckthorn biological feed, it is characterized in that: in step (3), the condition of culture of sea-buckthorn biological feed is the inoculum concentration of complex microorganism seed is 2 ~ 6%, and fermentation condition is 30 ± 5 DEG C of fermentations 36 ~ 72 hours.
6. complex microbial community fermentation according to claim 1 is for the method for sea-buckthorn biological feed, it is characterized in that: in step (4), the aftertreatment technology of sea-buckthorn biological feed is for drying, and be crushed to 40 ~ 1000 orders according to the purposes difference of biological feedstuff, quantitative separating gets product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510600355.9A CN105028896A (en) | 2015-09-21 | 2015-09-21 | Method for preparing sea-buckthorn biological fodder through compound microbial flora fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510600355.9A CN105028896A (en) | 2015-09-21 | 2015-09-21 | Method for preparing sea-buckthorn biological fodder through compound microbial flora fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105028896A true CN105028896A (en) | 2015-11-11 |
Family
ID=54436363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510600355.9A Pending CN105028896A (en) | 2015-09-21 | 2015-09-21 | Method for preparing sea-buckthorn biological fodder through compound microbial flora fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105028896A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997963A (en) * | 2018-11-28 | 2019-07-12 | 汪敬富 | A kind of sea-buckthorn organic feed |
| CN112753897A (en) * | 2021-01-29 | 2021-05-07 | 珠海容川饲料有限公司 | Penaeus vannamei compound feed utilizing fermented sea-buckthorn pomace and application thereof |
-
2015
- 2015-09-21 CN CN201510600355.9A patent/CN105028896A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997963A (en) * | 2018-11-28 | 2019-07-12 | 汪敬富 | A kind of sea-buckthorn organic feed |
| CN112753897A (en) * | 2021-01-29 | 2021-05-07 | 珠海容川饲料有限公司 | Penaeus vannamei compound feed utilizing fermented sea-buckthorn pomace and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103478413B (en) | Method for producing protein feed by mixed-strain solid-state fermentation of ginkgo leaf residues | |
| CN101297677B (en) | Technique for detoxicating rapeseed dregs and modifying rapeseed proteins by synchronous bacterium mixing and fermenting method | |
| CN103609862B (en) | Method for improving feeding nutritive value of sesame seed meal by combining enzymolysis method with fermentation method | |
| CN102823726A (en) | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity | |
| CN104557339A (en) | Preparation method of soluble fish protein ammonia acid fertilizer | |
| CN102948614A (en) | Method for preparing peptide used for active feed by bacteria and enzyme synergistic fermentation bean pulp | |
| CN105494890A (en) | Enzymolysis and fermentation combined method for improving feeding nutritional value of rapeseed meal | |
| CN104757267A (en) | Apple pomace microbial culture starter and method for producing biological feed by apple pomace microbial culture starter | |
| CN102660436A (en) | Oyster yellow wine | |
| CN104286406A (en) | Orange peel feed production method | |
| CN101444252A (en) | Method for preparing fermented virus-free cottonseed meal feed protein | |
| CN104273378A (en) | New process for producing fermented feed for dairy cows by taking wheat straw and distillers' grains as raw materials | |
| CN104012787A (en) | Method for preparing corn straw coarse feed by microbial beneficial living bacteria | |
| CN102187939A (en) | Method for producing health feed by using notoginseng residues fermenting | |
| CN101665373A (en) | Safe and high-efficiency preparation method of master culture medium material of edible fungi | |
| CN104381607A (en) | Phycomycete complex fermented feed additive and preparation method thereof | |
| CN104336301A (en) | Biological fermentation protein feed and preparation method thereof | |
| CN105231014A (en) | Microbiological feed | |
| CN102754732A (en) | Microbial fermented feed production method adopting multi-fungus joint transformation | |
| CN101120719A (en) | Microorganism ferment purple sweet potato and preparation method thereof | |
| CN104855674A (en) | Production method for microbial fermentation complete feed by combining strain joint transformations | |
| Aslamyah et al. | Fermentation of seaweed flour with various fermenters to improve the quality of fish feed ingredients | |
| CN103892052A (en) | Cordyceps militaris medium immunopotentiator for feeding and production method of immunopotentiator | |
| CN106036239A (en) | High-protein crab feed additive for raising feed utilization rate | |
| CN105028896A (en) | Method for preparing sea-buckthorn biological fodder through compound microbial flora fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151111 |
|
| WD01 | Invention patent application deemed withdrawn after publication |