CN105027731B - A kind of method broken berchemia lineata seed dormancy and promote it to germinate - Google Patents
A kind of method broken berchemia lineata seed dormancy and promote it to germinate Download PDFInfo
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- CN105027731B CN105027731B CN201510396328.4A CN201510396328A CN105027731B CN 105027731 B CN105027731 B CN 105027731B CN 201510396328 A CN201510396328 A CN 201510396328A CN 105027731 B CN105027731 B CN 105027731B
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Abstract
The invention provides a kind of method broken berchemia lineata seed dormancy and promote it to germinate, comprise the following steps：S1. berchemia lineata ripening fruits is plucked, progress takes kind；S2. gained berchemia lineata seed in S1 steps and wet sand are mixed, refrigeration；S3. the mixture in S2 steps by refrigeration is taken out, carries out the method that Pregermination and seedling breeding is provided according to the present invention, seed starts germination percentage in germination, one week in the 3rd d and terminated substantially up to 85% or so, 10 d germinations, and germination percentage is up to 90%.The inventive method material therefor is easily obtained, and easy to operate, safety, cost is low, is suitable for vast common peasant household's artificial breeding, to realize that berchemia lineata artificial propagation provides important technical support.
Description
Technical field
The invention belongs to berchemia lineata method for cultivating seeds technical field, berchemia lineata seed is broken more particularly, to one kind
Dormancy and the method for promoting it to germinate.
Background technology
Berchemia lineata, former plant is Rhamnaceae plant berchemia lineataBerchemia lineata(L.) DC. or littleleaf supplejackherbaB.polyphylla Wall var.leiocladaHandel-Mazzetti, is used as medicine with dry root or stem rattan more, is normal simply
Ethnic drug.In recent years, because it is found that berchemia lineata has very well in the many-side such as anti-inflammatory, analgesia, antibacterial, liver protection, antitumor
Curative effect, be exploited more and more, its market demand is significantly increased.At present, berchemia lineata crude drug source is essentially wild
Excavate, and resource reserves are gradually decreased, the few berchemia lineata certified products commodity medicinal materials circulations of in the market, are generally its substitute.From
Under the conditions of so, berchemia lineata result is more, but fruit lands the few of energy germinating to seedling, and generally nature germination percentage is only 3% or so.
There are some researches show, berchemia lineata pulp, pericarp, plant skin, embryo and there is the material that suppresses germination, at the same exist it is physical and
Physiological dormancy, causes its germination difficult.
Current prior art contributes to break its hibernation feature using the concentrated sulfuric acid or gibberellin, improves germinating energy, but dense sulphur
It is more difficult in sour actual production to obtain, and be present risk and safety issue in operation, should not use.Berchemia lineata seed is needed without processing
Want just to start to germinate successively for one week or so, and it is extremely irregular to germinate, time span is brought very more than 35 d to actual artificial breeding
Big adverse effect.Though prior art can be by germinating energy using 500 mg/L gibberellin（15th d counts germinating energy）It is promoted to 48%
Left and right, germination percentage is still longer the time required to being promoted to 66%, but germination, and germination regularity also has much room for improvement, for peasant household,
The gibberellin that certain actual concentrations are configured in actual production is also inconvenient.Therefore, further simplify, optimization promotes berchemia lineata seed
The method of germination, particularly improves germination regularity, shortens germinating time to realizing berchemia lineata artificial propagation, expand herb resource
It is particularly significant.
The content of the invention
It is an object of the invention to break berchemia lineata there is provided one kind according to the deficiency of existing berchemia lineata seed breeding technique
Seed dormancy and the method for promoting it to germinate.
The above-mentioned purpose of the present invention is achieved through the following technical solutions：
The invention provides a kind of method broken berchemia lineata seed dormancy and promote it to germinate, comprise the following steps：
S1. berchemia lineata ripening fruits is plucked, progress takes kind；
S2. gained berchemia lineata seed in S1 steps and wet sand are mixed, refrigeration；
S3. the mixture in S2 steps by refrigeration is taken out, carries out Pregermination and seedling breeding.
Closer, berchemia lineata ripening fruits is berchemia lineata in the S1Berchemia lineata(L.) DC. or light
Branch hooks catechuB.polyphylla Wall var.leioclada。
Seed dormancy types are broadly divided into three kinds of exogenous, endogenous and combinational dormancy, and exogenous dormancy is beyond embryo
Various tissues, including kind of shell（Plant skin, pericarp or endosperm etc.）Limitation, such as plant shell machinery hinder, waterproof, air impermeability with
And there is germination inhicbitor etc. in kind shell.Cause factor of the factor i.e. embryo of endogenous dormancy itself, sent out mainly due to embryo form
Educate unfinished, physiologically prematurity, lacking hormone or must have the material of Inhibiting germination, combinational dormancy is a variety of exogenous stops
The combination slept with endogenous dormancy.
Between different vegetable seeds, due to the presence of molecular genetic basic characteristic, each not phase of its hibernation feature is caused
Together.
For vegetable seeds dormancy situation Producing reason and restructuring bankrupt, because the dormancy and sprouting of vegetable seeds are one
Individual permanent numerous and diverse process, all kinds of link complexity therein are various, therefore, specifically how to handle, and which kind of side of abolishing taken
Method, to its rest period abolish and budding has large effect.
The present invention can effectively break the rest period of berchemia lineata seed, seed is in low tempertaure storage using the processing of low temperature wet sand
Germination percentage and germinating energy show good situation after 60 ~ 150d, and the step operation is simple, suitable for large area
Promote, there is great meaning for berchemia lineata artificial propagation.
Preferably, berchemia lineata seed is carried out disinfection processing after being taken in the S1 steps kind, it is described to disinfect to use
0.3%KMnO4Solution soaks 30 min.
Preferably, wet sand is sterile-processed in the S2 steps, disinfects to boil more than 25min.
Preferably, the wet sand and the mixing ratio of seed, are calculated as 3 ~ 5 by weight:1, the humidity of wet sand is 50% ~ 90%.
Preferably, the berchemia lineata ripening fruits described in the S1 is berchemia lineataBerchemia lineata(L.) during DC., S2
Refrigerated storage temperature is 0 ~ 7 DEG C in step, and cold preservation time is 60 ~ 120d.
The berchemia lineata ripening fruits described in the S1 is littleleaf supplejackherbaB.polyphylla Wall var.leioclada.
When, refrigerated storage temperature is 0 ~ 7 DEG C in S2 steps, and cold preservation time is 60 ~ 150d.
Preferably, Pregermination and seedling breeding is that clear water is steeped for after the mixture 24h of refrigeration in the S3 steps, is placed in germination base
In matter, and fine sand is covered, the cladding thickness of fine sand is not more than 1cm.
Compared with prior art, the present invention has advantages below and beneficial effect：
The method berchemia lineata seed that the present invention is provided germination percentage in 10 d reaches 90%, and germinating energy is promoted to 86%（7th d
Calculate germinating energy）, berchemia lineata germination regularity and germination percentage are greatly enhanced, shortens its sprout time.This hair
Bright method material therefor is easily obtained, and easy to operate, safety, cost is low, is suitable for vast common peasant household's artificial breeding, to realize iron
Artificial propagation covered with gold leaf provides important technical support, and the protection of berchemia lineata wild resource and sustainable development are utilized with important
Meaning.
Brief description of the drawings
Fig. 1 is the method and step schematic diagram that the embodiment of the present invention 1 is provided.
Embodiment
This technology is further illustrated below in conjunction with specific embodiment, but embodiment does not do any type of to this technology
Limit.Unless stated otherwise, the reagent of the invention used, method and apparatus is the art conventional reagent, methods and apparatus.
The berchemia lineata seed used in this experiment, is berchemia lineataBerchemia lineata(L.) DC. ripening fruits
Take and plant or littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Embodiment 1
1. choose no disease and pests harm, atropurpureus berchemia lineata fruit（Green should not never be plucked until erythrocarpus, it is
Seed is not developed completely in immature fruit, this growth phase fruit, can influence normal germination）.
The berchemia lineata seed that embodiment 1 is used forBerchemia lineata(L.) DC. ripening fruits takes kind.
2. the fruit that (1) step plucks is taken advantage of into fresh stranding removes skin and flesh, clear water is by the remaining skin and flesh and its juice that adhere on seed
Rinse well.
3. use 0.3%KMnO4The seed that (2) solution handle step soaks 30 min to sterilize, and clear water is rinsed well.
4. with sterilized（Simple and easy method：Fine sand is put into water and boils 25 more than min）The wet sand cooled down afterwards, with step
(3) the seed handled is mixed.It is required that husky humidity is 50% ~ 90%, seed is with husky volume ratio 1:3~1:Between 5.
5. the seed that (4) step is handled is put into 0 ~ 7 DEG C of fridge freshness retaining layer with husky mixture, 60d is refrigerated.
6. the seed that (5) step is handled takes out, normal temperature clear water is placed on Germination textures after soaking 24 h, is covered one layer thin
Fine sand（It is advisable with loseing seed, thickness is no more than 1 cm）, water, until keeping matrix moistening before germination.
Embodiment 2
Method and berchemia lineata seed be the same as Example 1, the difference is that step（5）In cold preservation time be 90d.
Embodiment 3
Method and berchemia lineata seed be the same as Example 1, the difference is that step（5）In cold preservation time be 120d.
Embodiment 4
Method and berchemia lineata seed be the same as Example 2, the difference is that without step（3）In KMnO4Solution disinfection processing.
Comparative example 1
Method and berchemia lineata seed be the same as Example 2, the difference is that step（5）In without wet sand mixing handle, directly it is low
Temperature is preserved.
Comparative example 2
Method and berchemia lineata seed be the same as Example 1, the difference is that step（5）In cold preservation time be 150d.
Comparative example 3
Method and berchemia lineata seed be the same as Example 2, the difference is that step（5）In without wet sand mixing handle, directly often
Preserved under warm drying condition.
Comparative example 4
Concentrated sulfuric acid corrosion treatment：Berchemia lineata seed be the same as Example 2, with reference to the step of embodiment 2, and in step（4）It is preceding by iron
Seed covered with gold leaf is without mixing wet sand, and normal temperature is handled, and 98% dense sulfuric acid treatment berchemia lineata seed 15min is used after taking-up,
After the completion of immersion, rinsed well with running water, and Seed germination is carried out with reference to the step 6 of embodiment 1.
Comparative example 5
With reference to the situation of comparative example 4, low-temperature treatment is carried out, remaining condition is constant.
Comparative example 6
Gibberellin processing：Berchemia lineata seed be the same as Example 2, with reference to the step of embodiment 2, and in step（4）It is preceding by berchemia lineata
Seed is without mixing wet sand, and normal temperature is handled, and berchemia lineata seed 6h is handled using 500mg/L gibberellin after taking-up, is soaked
After the completion of bubble, rinsed well with running water, and Seed germination is carried out with reference to the step 6 of embodiment 1.
Comparative example 7
With reference to the situation of comparative example 6, low-temperature treatment is carried out, remaining condition is constant.
Table 1 below is the berchemia lineata that the method processing that embodiment 1 ~ 4 and comparative example 1 ~ 7 are provided is obtainedBerchemia lineata(L.) germination percentage of DC. seeds and germinating energy situation：
Table 1：Berchemia lineataBerchemia lineata(L.) DC. seed treatments situation
Embodiment 5：With reference to the step of embodiment 1 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Embodiment 6：With reference to the step of embodiment 2 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Embodiment 7：With reference to the step of embodiment 3 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Embodiment 8：With reference to the step of embodiment 4 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Embodiment 9：With reference to the step of embodiment 3 and method, the difference is that step（5）In cold preservation time be 150d, iron bag
Gold seeds is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Comparative example 8：With reference to the step of comparative example 1 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Comparative example 9：With reference to the step of comparative example 3 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Comparative example 10：With reference to the step of comparative example 4 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Comparative example 11：With reference to the step of comparative example 5 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Comparative example 12：With reference to the step of comparative example 6 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Comparative example 13：With reference to the step of comparative example 7 and method, the difference is that berchemia lineata seed is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaTake kind.
Table 2 below is the littleleaf supplejackherba berchemia lineata seed that the method processing that embodiment 5 ~ 9 and comparative example 8 ~ 13 are provided is obtained
Germination percentage and germinating energy situation：
Table 2：Littleleaf supplejackherbaB.polyphylla Wall var.leiocladaSeed treatment situation
From the point of view of embodiment 1 and the situation of embodiment 4, ensure rinse well in the case of, by sterilization with without too high
The comparison of potassium manganate sterilization, to berchemia lineataBerchemia lineata(L.) DC. seeds breaking dormancy phase and germination have one
It is fixed to help.Embodiment 8 and embodiment 5 also illustrate that the situation.
For berchemia lineataBerchemia lineata(L.) DC. seeds, the method provided by the present invention, at 90d
After reason, preferable budding situation, its germination percentage % are obtained（10d is calculated）For 90%, germinating energy %
（7th d is calculated）For 86%；For littleleaf supplejackherbaB.polyphylla Wall var.leiocladaSeed,
After 120d, preferably budding effect, its germination percentage of its germination percentage % are achieved（10d is calculated）For 86%, germinating energy %（7th d
Calculate）For 81%.But after continuing to be kept for its rest period reach 150d, its germination percentage and germinating energy are significantly reduced.
And independent low-temperature treatment, its germination of the seed of gained is unsatisfactory, substantially less than the effect of the present embodiment 1 ~ 9
Really, as it was previously stated, between different vegetable seeds, due to the presence of molecular genetic basic characteristic, causing its hibernation feature each
Differ, all kinds of link complexity therein are various, specific processing method can be to dormancy characteristics of different, and germination feelings to after
Condition is affected greatly, and the comparative example is to illustrate such case, meanwhile, the present invention is also carried out with reference to the method for embodiment 2
Room temperature wet sand storage test, discovery begins to germinate successively by 15 d stored seedses, but stored seedses easily go rotten under the conditions of this,
The seed individual difference reason such as cause germination regularity not good, this method should not be used in actual production.
In addition, be directed to it is reported that, by the concentrated sulfuric acid and gibberellin processing after, the germination to breaking berchemia lineata seed
Situation results in more positive effect, and the present invention have also been made related trial, is specifically under room temperature and cryogenic conditions, respectively
The berchemia lineata germination situation after the concentrated sulfuric acid and gibberellin processing is determined, Tables 1 and 2, comprehensive related data is specifically shown in
Show, although low-temperature treatment, have certain effect with reference to the concentrated sulfuric acid and gibberellin, but be still significantly lower than the embodiment of the present invention 1 ~ 9
Germination, it is impossible to reach and realize that berchemia lineata is artificial quick, amount reproduction, and purpose simple to operate.
Show based on the above results, low temperature Sand culture was largely effective to releasing berchemia lineata seed dormancy, at 60 ~ 150d weeks
After phase processing, 0 ~ 7 DEG C of low temperature sand storage can break seed dormancy completely, and operating method is easy, be particularly suitable in actual production a large amount of
Seed artificial propagation.
Claims (3)
1. a kind of method broken berchemia lineata seed dormancy and promote it to germinate, it is characterised in that comprise the following steps：
S1. berchemia lineata ripening fruits is plucked, progress takes kind；
S2. gained berchemia lineata seed in S1 steps and wet sand are mixed, refrigeration；
S3. the mixture in S2 steps by refrigeration is taken out, carries out Pregermination and seedling breeding；
Berchemia lineata ripening fruits is berchemia lineata in the S1Berchemia lineata(L.) DC. or littleleaf supplejackherbaB.polyphylla Wall var.leioclada；
The berchemia lineata ripening fruits described in the S1 is berchemia lineataBerchemia lineata(L.) during DC., temperature is refrigerated in S2 steps
Spend for 0 ~ 7 DEG C, cold preservation time is 60 ~ 120d；
The berchemia lineata ripening fruits described in the S1 is littleleaf supplejackherbaB.polyphylla Wall var.leiocladaWhen, S2
Refrigerated storage temperature is 0 ~ 7 DEG C in step, and cold preservation time is 60 ~ 120d；
The mixing ratio of the wet sand and seed, is calculated as 3 ~ 5 by weight:1, the humidity of wet sand is 50% ~ 90%；In the S1 steps
Berchemia lineata seed is carried out disinfection processing after taking kind, it is described to disinfect to use 0.3%KMnO4Solution soaks 30 min.
2. the method according to claim 1 broken berchemia lineata seed dormancy and promote it to germinate, it is characterised in that described
Wet sand is sterile-processed in S2 steps.
3. the method according to claim 1 broken berchemia lineata seed dormancy and promote it to germinate, it is characterised in that described
Pregermination and seedling breeding is that clear water is steeped for after the mixture 24h of refrigeration in S3 steps, is placed on Germination textures, and covers fine sand, carefully
Husky cladding thickness is not more than 1cm.
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