CN104737667A - Method for increasing germination rate of Korla fragrant pear hybrid seeds - Google Patents

Method for increasing germination rate of Korla fragrant pear hybrid seeds Download PDF

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Publication number
CN104737667A
CN104737667A CN201510117501.2A CN201510117501A CN104737667A CN 104737667 A CN104737667 A CN 104737667A CN 201510117501 A CN201510117501 A CN 201510117501A CN 104737667 A CN104737667 A CN 104737667A
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China
Prior art keywords
seed
seeds
river sand
germination rate
clean
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CN201510117501.2A
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Chinese (zh)
Inventor
刘永杰
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Korla City Bergamot Pear Research Center
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Korla City Bergamot Pear Research Center
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Priority to CN201510117501.2A priority Critical patent/CN104737667A/en
Publication of CN104737667A publication Critical patent/CN104737667A/en
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Abstract

The invention discloses a method for increasing the germination rate of Korla fragrant pear parental hybrid seeds. The method comprises the following steps: picking full ripe hybrid fruits, placing the fruits in a cold storage at a temperature ranging from -1.5 to 0 DEG C for carrying out after-ripening, taking out the fruits from the cold storage, taking seeds, cleaning the seeds taken out, drying in the shade, soaking the seeds in warm water at 55 DEG C, then soaking for 12 hours when the temperature of water is naturally lowered to room temperature, then fishing out the seeds, cleaning by clear water, carrying out seed soaking disinfection on the hybrid seeds for 5-10 minutes by 0.5% potassium permanganate, and depositing the seeds by disinfected clean river sand, uniformly mixing and stirring the treated seeds with the river sand in a ratio of 1:5, tightly wrapping by cotton gauze with good breathability, then covering with the treated river sand, and refrigerating in a refrigerator at a temperature of 4 DEG C. According to the method disclosed by the invention, the germination condition is checked when the seeds are deposited for about 50 days, and the germination rate of the seeds can be up to 85% and above when the seeds are deposited for 55-60 days.

Description

A kind of method improving Kuerle delicious pear hybrid seed germination rate
Technical field
The invention belongs to field of plant variety breeding technology, be specifically related to a kind of method improving Kuerle delicious pear hybrid seed germination rate.
Background technology
Kuerle delicious pear originates in Xinjiang, China Kuerle.The thin meat of this bergamot pear skin is thin, and juice multi-flavor is sweet, crisp tasty and refreshing; Pole storage tolerance and the advantage such as region is extremely strong.Ripe fruit in season face is yellowish green, and sunny side has blush, becomes cadmium yellow after storage, and the micro-band of pulp white is green, and meat is tenderly white, and quality is crisp, and juice is how dense sweet, aromatic flavour.According to science assay, Kuerle delicious pear is of high nutritive value, sugar content 10%, acid 0.03%, ash content 0.12%, and every 100 grams of bergamot pears are containing vitamin C about 4.3 milligrams, and edible part reaches 83.6%.
Applicant is that breeding parent material has done a large amount of crossbreeding work, for the sustainable development of Kuerle delicious pear breed improvement and bergamot pear industry provides support with Kuerle delicious pear.Applicant obtains a kind of method improving Kuerle delicious pear hybrid seed germination rate by hybrid seeding, yet there are no the report of related fields.
Summary of the invention
The present invention aims to provide and a kind ofly improves the method that Kuerle delicious pear is the hybrid seed germination rate of parent.
The present invention is achieved through the following technical solutions:
Improve a method for Kuerle delicious pear hybrid seed germination rate, it mainly comprises the following steps:
1) pluck: pluck after hybridization fruit maturation, for obtaining ripe seed;
2) after-ripening: be placed in time in freezer by the fruit after harvesting and carry out after-ripening, the temperature of freezer controls between-1.5 DEG C ~ 0 DEG C;
3) clean: fruit is got kind after freezer takes out, getting in the process of planting the seed needing to remove flat grain and breakage, and the seed taken out is cleaned, dried in the shade;
4) hot water treatment of seeds: warm water seed being put into 50-55 DEG C soaks, naturally cool to after room temperature until water temperature and soak 12 hours again, then pull seed out and clean with clear water;
5) seed treatment: with 0.5% potassium permanganate, the hybrid seed after soaking is carried out disinfection 5-10 minute, pull seed out and rinse with clear water clean;
6) lamination: the seed processed and river sand are mixed in the ratio of 1:5 and mixes thoroughly, tight with the cotton gauze parcel of good permeability, then the river sand after Mulching treatment, put into refrigerator cold-storage, temperature controls at 4 DEG C.
The standard of described seed maturity is that seed coat is sent out brown, and seed is full.
Described ripening time is 110 ~ 130 days.
Described river sand clean water 2-3 time, washes away alkali, soil, then carries out disinfection to it with boiling water, has controlled moisture content and again river sand has been spread out the moon and dry in the air, and grabbing agglomerating, loose the opening of hand when river sand hand can carry out lamination.
The inventive method earthing of seeds started to check germination by about 50 days, and as lamination 55-60 days, percentage of seedgermination can reach more than 85%.
Beneficial effect of the present invention is: prior art is after fruit maturation, place at room temperature, Deng fruit completely rot after take out seed, and then carry out sand Tibetan, the hybrid seed germination rate that this method obtains is generally between 10% ~ 20%, the innovation of technology of the present invention is, ripening fruits after harvesting is first placed in freezer and carries out low temperature treatment, get after planting seed disinfection, again seed is placed on constant low temperature and carries out lamination, the germination rate of hybrid seed can be made to bring up to more than 85%.
Embodiment
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed to the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, according to technical spirit of the present invention to any simple modification made for any of the above embodiments or equivalent variations, all drop in protection scope of the present invention.
Embodiment 1
Improve a method for Kuerle delicious pear hybrid seed germination rate, it mainly comprises the following steps:
1) in order to ensure the maturity of seed, pluck after hybridization fruit full maturity, general plucking time is by the end of August to September 20, and the standard of seed maturity is that seed coat is sent out brown, and seed is full;
2) after-ripening: the fruit after harvesting will be placed in time in freezer and carry out after-ripening, and the temperature of freezer controls at-1 DEG C, and ripening time is 120 days.
3) clean: fruit is got kind after freezer takes out, getting the flat grain of timely removal and damaged seed in the process of planting, and the seed of taking-up is cleaned, dried in the shade.
4) hot water treatment of seeds: warm water seed being put into 50-55 DEG C soaks, naturally cool to after room temperature until water temperature and soak 12 hours again, then pull seed out and clean with clear water.
5) seed treatment: seed 0.5% potassium permanganate is carried out seed soaking sterilization 5-10 minute to hybrid seed, and carry out lamination with clean river sand, river sand clean water 2 ~ 3 times, wash away alkali, soil, then with boiling water, it is carried out disinfection, controlled moisture again river sand to be spread out the moon and dry in the air, when river sand hand grab agglomerating, hand is loose opens.
6) lamination: the seed processed and river sand are mixed in the ratio of 1:5 and mixes thoroughly, tight with the cotton gauze parcel of good permeability, then the river sand after Mulching treatment, put into refrigerator cold-storage, temperature controls at 4 DEG C.
The method earthing of seeds started to check germination by 50 days, and as lamination 55-60 days, percentage of seedgermination can reach 85%.
Embodiment 2
Improve a method for Kuerle delicious pear hybrid seed germination rate, it mainly comprises the following steps:
1) in order to ensure the maturity of seed, pluck after hybridization fruit full maturity, general plucking time is by the end of August to September 20, and the standard of seed maturity is that seed coat is sent out brown, and seed is full;
2) after-ripening: the fruit after harvesting will be placed in time in freezer and carry out after-ripening, the temperature of freezer controls at 0 DEG C, and ripening time is 130 days.
3) clean: by fruit from after freezer takes out, i.e. desirable kind, removes flat grain and damaged seed in time, and clean the seed taken out, dry in the shade in the process of to get kind.
4) hot water treatment of seeds: warm water seed being put into 50-55 DEG C soaks, naturally cool to after room temperature until water temperature and soak 12 hours again, then pull seed out and clean with clear water.
5) seed treatment: seed 0.5% potassium permanganate is carried out seed soaking sterilization 5-10 minute to hybrid seed, and carry out lamination with clean river sand, river sand clean water 2-3 time, wash away alkali, soil, then with boiling water, it is carried out disinfection, controlled moisture again river sand to be spread out the moon and dry in the air, when river sand hand grab agglomerating, hand is loose opens.
6) lamination: the seed processed and river sand are mixed in the ratio of 1:5 and mixes thoroughly, tight with the cotton gauze parcel of good permeability, then the river sand after Mulching treatment, put into refrigerator cold-storage, temperature controls at 4 DEG C.
The method earthing of seeds started to check germination by 48 days, and as lamination 55-60 days, percentage of seedgermination can reach 90%.
Embodiment 3
Improve a method for Kuerle delicious pear hybrid seed germination rate, it mainly comprises the following steps:
1) in order to ensure the maturity of seed, pluck after hybridization fruit full maturity, general plucking time is by the end of August to September 20, and the standard of seed maturity is that seed coat is sent out brown, and seed is full;
2) after-ripening: the fruit after harvesting will be placed in time in freezer and carry out after-ripening, and the temperature of freezer controls at-1.5 DEG C, and ripening time is 110 days.
3) clean: by fruit from after freezer takes out, i.e. desirable kind, removes flat grain and damaged seed in time, and clean the seed taken out, dry in the shade in the process of to get kind.
4) hot water treatment of seeds: warm water seed being put into 50-55 DEG C soaks, naturally cool to after room temperature until water temperature and soak 12 hours again, then pull seed out and clean with clear water.
5) seed treatment: seed 0.5% potassium permanganate is carried out seed soaking sterilization 10 minutes to hybrid seed, and carry out lamination with clean river sand, river sand clean water 2-3 time, wash away alkali, soil, then with boiling water, it is carried out disinfection, controlled moisture again river sand to be spread out the moon and dry in the air, when river sand hand grab agglomerating, hand is loose opens.
6) lamination: the seed processed and river sand are mixed in the ratio of 1:5 and mixes thoroughly, tight with the cotton gauze parcel of good permeability, then the river sand after Mulching treatment, put into refrigerator cold-storage, temperature controls at 4 DEG C.
The method earthing of seeds started to check germination by 45 days, and as lamination 55-60 days, percentage of seedgermination can reach 95%.

Claims (4)

1. improve a method for Kuerle delicious pear hybrid seed germination rate, it is characterized in that: comprise the following steps:
1) pluck: pluck after hybridization fruit full maturity, obtain ripe seed;
2) after-ripening: be placed in time in freezer by the fruit after harvesting and carry out after-ripening, the temperature of freezer controls between-1.5 DEG C ~ 0 DEG C;
3) clean: fruit is got kind after freezer takes out, getting the flat grain of timely removal and damaged seed in the process of planting, and the seed of taking-up is cleaned, dried in the shade;
4) hot water treatment of seeds: warm water seed being put into 55 DEG C soaks, naturally cool to after room temperature until water temperature and soak 12 hours again, then pull seed out and clean with clear water;
5) seed treatment: seed 0.5% potassium permanganate is carried out seed soaking sterilization 5-10 minute to hybrid seed, pulls seed out and rinse with clear water clean;
6) lamination: the seed processed and river sand are mixed in the ratio of 1:5 and mixes thoroughly, tight with the cotton gauze parcel of good permeability, then the river sand after Mulching treatment, put into refrigerator cold-storage, temperature controls at 4 DEG C.
2. a kind of method improving Kuerle delicious pear hybrid seed germination rate according to claim 1, is characterized in that: the standard of described seed maturity is that seed coat is sent out brown, and seed is full.
3. a kind of method improving Kuerle delicious pear hybrid seed germination rate according to claim 1, is characterized in that: described ripening time is 110 ~ 130 days.
4. a kind of method improving Kuerle delicious pear hybrid seed germination rate according to claim 1, it is characterized in that: the clean river sand after described sterilization is for using clean water 2-3 time, wash away alkali, soil, then with boiling water, it is carried out disinfection, controlled moisture again river sand to be spread out the moon and dry in the air, grabbing agglomerating, loose the opening of hand when river sand hand can carry out lamination.
CN201510117501.2A 2015-03-17 2015-03-17 Method for increasing germination rate of Korla fragrant pear hybrid seeds Pending CN104737667A (en)

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Application Number Priority Date Filing Date Title
CN201510117501.2A CN104737667A (en) 2015-03-17 2015-03-17 Method for increasing germination rate of Korla fragrant pear hybrid seeds

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106105480A (en) * 2016-07-01 2016-11-16 甘肃省农业科学院林果花卉研究所 A kind of improve pears hybrid seed germination percentage and the method for planting percent
CN108293553A (en) * 2017-08-30 2018-07-20 中国农业科学院郑州果树研究所 A kind of pears seed sowing crossbreeds strong sprout method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
吴敏: "库尔勒香梨杂交育种试验", 《中国优秀硕士学位论文全文数据库》 *
王月志等: "早熟砂梨种子萌发的影响因素分析", 《浙江农业科学》 *
颜世萍等: "山梨培育技术", 《林业勘察设计》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106105480A (en) * 2016-07-01 2016-11-16 甘肃省农业科学院林果花卉研究所 A kind of improve pears hybrid seed germination percentage and the method for planting percent
CN106105480B (en) * 2016-07-01 2019-02-01 甘肃省农业科学院林果花卉研究所 A method of improving pears hybrid seed germination percentage and planting percent
CN108293553A (en) * 2017-08-30 2018-07-20 中国农业科学院郑州果树研究所 A kind of pears seed sowing crossbreeds strong sprout method

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Application publication date: 20150701

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