CN105021509B - A kind of overall water permeability tester of pulsating artificial blood vessel and its application method - Google Patents
A kind of overall water permeability tester of pulsating artificial blood vessel and its application method Download PDFInfo
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- CN105021509B CN105021509B CN201510418675.2A CN201510418675A CN105021509B CN 105021509 B CN105021509 B CN 105021509B CN 201510418675 A CN201510418675 A CN 201510418675A CN 105021509 B CN105021509 B CN 105021509B
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Abstract
The invention provides a kind of overall water permeability tester of pulsating artificial blood vessel and its application method.The overall water permeability tester of described pulsating artificial blood vessel, it is characterized in that, including water tank, the feed water inlet of water tank connects variable frequency pump by the first pipeline, the first joint that variable frequency pump is connected to be connected with one end of artificial blood vessel's sample by the second pipeline, the water return outlet of water tank is connected by the 3rd pipeline for the second joint that the other end with artificial blood vessel's sample is connected, wherein, the second described pipeline occurs module with pulsation and is connected.The present invention can be realized and tested under various waveforms, various pressure stages, and true transplanting environment, closing to reality service condition are simulated well.
Description
Technical field
The present invention relates to a kind of overall water permeability tester of pulsating artificial blood vessel and its application method, belong to Medical treatment device
Tool test device field.
Background technology
With the development of human society, vascular diseases are always the first big killer of human health.The appearance of vasotransplantation
A solution is provided with surgical operation therapy vascular diseases are developed into.At present, artificial blood vessel is successfully applied to clinic, solution
The problems such as shortage of biological species of having determined grafting vessel, rejection, have been achieved with good social benefit and considerable economic benefit.According to
International standard ISO 7198-1998 provide that artificial blood vessel must carry out water permeability test before transplantation, and water permeability is necessary
Meeting clinical requirement can transplant.
At present, test artificial blood vessel's water permeability has two ways:Water permeability and overall water permeability.In other words,
Can be circular flat sample (test of correspondence water permeability) or tubulose sample when artificial blood vessel tests water permeability
Unit chronomere under (test of correspondence entirety water permeability), test 16.0kPa ± 0.3kPa (120mmHg ± 2mmHg) pressure
The water percolating capacity of area.It is clear that overall water permeability test is more nearly the transplanting environment of artificial blood vessel, it is as a result more objective
Truly.
However, the vascular pressure force value of human body different parts is different, and there is systolic pressure and diastolic pressure, not single pressure
Force value.Therefore, it is also different to be transplanted to the pressure that the artificial blood vessels of different parts born.In order to emulate mould well
Intend the environment after synthetic vascular grafts, and more scientifically test its overall water permeability, the present invention proposes a kind of pulsating people
Work blood vessel entirety water permeability tester.Through retrieval, the overall water of simulation pulsating condition test artificial blood vessel there is no both at home and abroad at present
Infiltrative special purpose device or instrument.
The content of the invention
The technical problems to be solved by the invention be to provide a kind of overall water permeability tester of pulsating artificial blood vessel and
Its application method, on the one hand arbitrarily can adjust pressure in the range of 0-30kPa;On the other hand, it is possible to provide the pulsation of certain waveform.
2 points of innovation, can more truly simulate the transplanting environment of artificial blood vessel, to the overall water penetration of artificial blood vessel more than
Property has an objective appraisal.
In order to solve the above problems, the invention provides a kind of overall water permeability tester of pulsating artificial blood vessel, its
It is characterised by, including water tank, the feed water inlet of water tank is by the first pipeline connection variable frequency pump, and variable frequency pump passes through the second pipeline and connects use
The first joint being connected in one end with artificial blood vessel's sample, the second joint being connected for the other end with artificial blood vessel's sample
The water return outlet of water tank is connected by the 3rd pipeline, wherein, the second described pipeline occurs module with pulsation and is connected.
Preferably, module, which occurs, for described pulsation includes horizontal movement motor, computer supervision equipment and metal bellows, institute
The computer supervision equipment connection horizontal movement motor stated, horizontal movement motor can drive metal under the control of computer supervision equipment
Bellows carries out horizontal movement to form pulsating wave, and metal bellows connects the second pipeline.
Preferably, pressure sensor is provided with the second described pipeline, module occurs for pressure sensor connection pulsation, and can
Real-time pressure value is fed back into pulsation to occur in module.
Preferably, the second described pipeline includes the first threeway and the second threeway, and the first port of the first threeway passes through pipe
Road connects variable frequency pump, and by pipeline connection pulsation module occurs for the second port of the first threeway, and the 3rd port of the first threeway leads to
The first port that pipeline connects the second threeway is crossed, pressure sensor is provided with the second port of the second threeway, the of the second threeway
The first joint that three ports are connected to be connected with one end of artificial blood vessel's sample by pipeline.
It is highly preferred that described pipeline is silicone tube.
Preferably, the first described pipeline and the 3rd pipeline are silicone tube.
Preferably, tongs is provided with the 3rd described pipeline.
Preferably, described water tank is the container of the storing liquid of metal or plastic material.
Preferably, described water tank is regular shape or irregular shape.
Preferably, the pump water of described variable frequency pump is controllable, so as to control hydraulic pressure in 0-30kPa scopes, control accuracy is
±0.1kPa。
Preferably, the overall water permeability tester of described pulsating artificial blood vessel also includes metering scales, described meter
Amount balance collects the liquid that oozes out of artificial blood vessel's sample and carries out continuous metering.
It is highly preferred that described metering scales are made up of transparent lucite.
It is highly preferred that described metering scales are including can artificial blood vessel be protected, prevent because pressure oscillation causes
Liquid enters the upper part being shot out and can be grouped into the funnel shaped bottom that the liquid oozed out is collected and is measured, and upper and lower two
Part is integrally formed or linked together, and the bottom of the lower part of metering scales is measurement zone.
Present invention also offers the application method of the overall water permeability tester of above-mentioned pulsating artificial blood vessel, its feature
It is, including:
Step 1:Editor needs the pulsating waveform being carried in artificial blood vessel's sample in module occurs for pulsation, sets and is expected
Fluctuation pressure scope;
Step 2:Artificial blood vessel's sample two ends are separately fixed at the first joint and second with suture by the water filling in water tank
On joint;
Step 3:Open variable frequency pump water supply start, the motion of regulation tongs and controlled level motion motor, until pressure
The pressure limit that sensor is measured is equal to expected fluctuation pressure scope;Metering scales are placed in below artificial blood vessel's sample,
The liquid volume read in metering scales for every 60 seconds, after 10-60 minutes, is calculated under the pressure, sample every square centimeter is per minute
Water percolating capacity average value and standard deviation, i.e. artificial blood vessel's sample overall water permeability;
Step 4:Variable frequency pump is closed, tongs is outwarded winding, unloads artificial blood vessel's sample, be completed.
Compared with prior art, the present invention has the advantages that:
1. the water tank in the overall water permeability tester of the pulsating artificial blood vessel of the present invention is the container of storing liquid, become
Fluid flow in frequency pump regulation pipeline, computer supervision equipment controlled level motion motor, driving metal bellows forms various ripples
The ripple of shape, pressure sensor feeds back to the real-time pressure value in circulating system in computer supervision equipment, by adjusting variable frequency pump
And tongs results in the pressure of any pressure stage, all modules are connected as closing by silicone tube and threeway etc.
Circulating system.The liquid oozed out from artificial blood vessel is flowed in metering scales, directly reads data, calculates artificial blood vessel's water penetration
Property.The present invention can be realized and tested under various waveforms, various pressure stages, and true transplanting environment is simulated well, presses close to reality
Border service condition;
2. the present invention is applied to all size, artificial blood vessel's sample of various molding modes.
3. the present invention devises metering scales, described metering scales are made up of two parts up and down, described artificial blood
Pipe sample is interior located at the top of metering scales point, and the bottom of metering scales is divided into infundibulate, can protect and collect to ooze out
Liquid, while data can in extremely rapid succession be read, it is to avoid change the error that causes of water container.
Brief description of the drawings
Fig. 1 is the overall water permeability tester schematic diagram of pulsating artificial blood vessel;
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments be merely to illustrate invention and
It is not used in limitation the scope of the present invention.In addition, it is to be understood that after the content of the invention lectured has been read, those skilled in the art
The present invention can be made various changes or modifications, these equivalent form of values equally fall within what the application appended claims were limited
Scope.
Embodiment 1
As shown in figure 1, being the overall water permeability tester schematic diagram of pulsating artificial blood vessel, the artificial blood of described pulsating
Pipe entirety water permeability tester includes water tank 1, and the feed water inlet of water tank 1 connects variable frequency pump 3 by the first pipeline, and variable frequency pump 3 leads to
Cross the first joint 11 that the second pipeline is connected to be connected with one end of artificial blood vessel's sample 13, for artificial blood vessel's sample 13
The other end connection the second joint 12 by the 3rd pipeline connect water tank 1 water return outlet, wherein, the second described pipeline and arteries and veins
It is dynamic to occur module connection.Module occurs for described pulsation by 5 groups of horizontal movement motor 4, computer supervision equipment 7 and metal bellows
Into, the described connection horizontal movement of computer supervision equipment 7 motor 4, horizontal movement motor 4 can computer supervision equipment 7 control
Lower driving metal bellows 5 carries out horizontal movement to form pulsating wave, and metal bellows 5 connects the second pipeline.
The second described pipeline includes the first threeway 14 and the second threeway 10, and the first port of the first threeway 14 passes through silica gel
Pipe connects variable frequency pump 3, and the second port of the first threeway 14 connects the metal bellows 5 that module occurs for pulsation by silicone tube, the
3rd port of one threeway 14 is connected by silicone tube to be set in the first port of the second threeway 10, the second port of the second threeway 10
There is pressure sensor 6, the computer supervision equipment 7 of module occurs for the connection pulsation of pressure sensor 6, and can feed back real-time pressure value
In the computer supervision equipment 7 that module occurs to pulsation.3rd port of the second threeway 10 by silicone tube be connected to manually
First joint 11 of one end connection of blood vessel sample 13.The first described pipeline and the 3rd pipeline are silicone tube.Described the 3rd
Tongs 9 is provided with pipeline.The pressure of any pressure stage is resulted in by adjusting variable frequency pump 3 and tongs 9.
The water tank is the container of the storing liquid of the plastic material of cylinder;The pump water of described variable frequency pump is controllable,
So as to control hydraulic pressure in 0-30kPa scopes, control accuracy is ± 0.1kPa.The overall water permeability of described pulsating artificial blood vessel
Tester also includes metering scales, and the liquid oozed out from artificial blood vessel is flowed in metering scales, directly reads data, calculates people
Work blood vessel water permeability.Described metering scales are made up of transparent lucite, including can artificial blood vessel be protected, and prevent
Only because pressure oscillation causes liquid to enter the upper part being shot out and can collect the liquid oozed out and carry out continuous metering
Funnel shaped bottom is grouped into, and upper and lower two parts are integrally formed, and the bottom of the lower part of metering scales is measurement zone.
Using the overall water permeability tester of above-mentioned pulsating artificial blood vessel, test under the conditions of 7-12kPa, pulsating waveform
For sine wave, interior diameter is 6mm, the operating procedure of the overall water permeability of long 70mm artificial blood vessel's sample is as follows:
Step 1:Editor needs to be carried in artificial blood vessel's sample 13 in the computer supervision equipment 7 that module occurs for pulsation
Pulsating waveform-sinusoidal waveform, it is 7-12kPa to set expected fluctuation pressure scope;
Step 2:Cleaning, water by filtering, at room temperature are filled in water tank 1, artificial blood vessel's sample (diameter is taken
6mm, long 70mm), the two ends of artificial blood vessel's sample 13 are separately fixed on the first joint 11 and the second joint 12 with suture, made
Obtain whole pipeline formation loop;
Step 3:The water supply start of variable frequency pump 3 is opened, the pressure value that observation pressure sensor 6 is measured adjusts tongs 9, made
Pressure value rises to 9.5kPa.Controlled level motion motor, makes metal bellows 5 do sinusoidal motion, metal bellows 5 does one simultaneously
Maintenance and operation is moved, and the direction of motion is parallel to the axial direction of metal bellows 5, the pressure limit that observation pressure sensor 6 is measured, until reaching
7-12kPa.Metering scales are placed in below artificial blood vessel's sample and start timing, the liquid read in metering scales for every 60 seconds
Volume.After 10 minutes, calculate under the pressure stage, the average value and standard deviation of sample every square centimeter amount of seepage per minute
The overall water permeability of difference, i.e. artificial blood vessel's sample;
Step 4:Variable frequency pump 3 is closed, tongs 9 is outwarded winding, unloads artificial blood vessel's sample 13, be completed.
It is computed, the overall water permeability of artificial blood vessel's sample is 70 ± 6.80mL/mincm2。
Embodiment 2
Using the overall water permeability tester of pulsating artificial blood vessel described in embodiment 1, test under the conditions of 11-16kPa,
Waveform be human body pulsating wave, interior diameter be 20mm, the overall water permeability of long 210mm artificial blood vessel's sample operating procedure such as
Under:
Step 1:Editor needs to be carried in artificial blood vessel's sample 13 in the computer supervision equipment 7 that module occurs for pulsation
Pulsating waveform-human pulse waveform, it is 11-16kPa to set expected fluctuation pressure scope;
Step 2:Cleaning, water by filtering, at room temperature are filled in water tank 1, artificial blood vessel's sample (diameter is taken
20mm, long 210mm), the two ends of artificial blood vessel's sample 13 are separately fixed on the first joint 11 and the second joint 12 with suture,
So that whole pipeline formation loop;
Step 3:The water supply start of variable frequency pump 3 is opened, the pressure value that observation pressure sensor 6 is measured adjusts tongs 9, made
Pressure value rises to 13.5kPa.Controlled level motion motor, makes metal bellows 5 do pulse wave motion, metal bellows simultaneously
5 do motion in one dimension, and the direction of motion is parallel to the axial direction of metal bellows 5, the pressure limit that observation pressure sensor 6 is measured, directly
To reaching 11-16kPa.Metering scales are placed in below artificial blood vessel's sample and start timing, metering scales are read within every 60 seconds
In liquid volume.After 10 minutes, calculate under the pressure stage, the average value of sample every square centimeter amount of seepage per minute and
The overall water permeability of standard deviation, i.e. artificial blood vessel's sample;
Step 4:Variable frequency pump 3 is closed, tongs 9 is outwarded winding, unloads artificial blood vessel's sample 13, be completed.
It is computed, the overall water permeability of artificial blood vessel's sample is 30 ± 4.54mL/mincm2。
Embodiment 3
Using the overall water permeability tester of pulsating artificial blood vessel described in embodiment 1, test under the conditions of 13-24kPa,
Waveform is square wave, and interior diameter is 10mm, the operating procedure of the overall water permeability of long 110mm artificial blood vessel's sample is as follows:
Step 1:Editor needs to be carried in artificial blood vessel's sample 13 in the computer supervision equipment 7 that module occurs for pulsation
Pulsating waveform-square wave, it is 13-24kPa to set expected fluctuation pressure scope;
Step 2:Cleaning, water by filtering, at room temperature are filled in water tank 1, artificial blood vessel's sample (diameter is taken
10mm, long 110mm), the two ends of artificial blood vessel's sample 13 are separately fixed on the first joint 11 and the second joint 12 with suture,
So that whole pipeline formation loop;
Step 3:The water supply start of variable frequency pump 3 is opened, the pressure value that observation pressure sensor 6 is measured adjusts tongs 9, made
Pressure value rises to 18.5kPa.Controlled level motion motor, makes metal bellows 5 do square wave motion, metal bellows 5 is done simultaneously
Motion in one dimension, the direction of motion is parallel to the axial direction of metal bellows 5, the pressure limit that observation pressure sensor 6 is measured, until reaching
To 13-24kPa.Metering scales are placed in below artificial blood vessel's sample and start timing, are read in metering scales within every 60 seconds
Liquid volume.After 10 minutes, calculate under the pressure stage, the average value and standard of sample every square centimeter amount of seepage per minute
The overall water permeability of deviation, i.e. artificial blood vessel's sample;
Step 4:Variable frequency pump 3 is closed, tongs 9 is outwarded winding, unloads artificial blood vessel's sample 13, be completed.
It is computed, the overall water permeability of artificial blood vessel's sample is 280 ± 15.44mL/mincm2。
Claims (10)
1. a kind of overall water permeability tester of pulsating artificial blood vessel, including water tank(1), it is characterised in that water tank(1)Confession
The mouth of a river connects variable frequency pump by the first pipeline(3), variable frequency pump(3)It is connected to and artificial blood vessel's sample by the second pipeline(13)
One end connection the first joint(11), for artificial blood vessel's sample(13)The other end connection the second joint(12)Pass through
3rd pipeline connects water tank(1)Water return outlet, wherein, described the second pipeline occurs module with pulsation and is connected.
2. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterised in that described pulsation
Generation module includes horizontal movement motor(4), computer supervision equipment(7)And metal bellows(5), described computer supervision equipment
(7)Connect horizontal movement motor(4), horizontal movement motor(4)Can be in computer supervision equipment(7)Control under drive wavy metal
Pipe(5)Horizontal movement is carried out to form pulsating wave, metal bellows(5)Connect the second pipeline.
3. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterised in that described second
Pressure sensor is provided with pipeline(6), pressure sensor(6)Module occurs for connection pulsation, and can feed back to real-time pressure value
Pulsation occurs in module.
4. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterised in that described second
Pipeline includes the first threeway(14)With the second threeway(10), the first threeway(14)First port variable frequency pump is connected by pipeline
(3), the first threeway(14)Second port pass through pipeline connection pulsation occur module, the first threeway(14)The 3rd port pass through
Pipeline connects the second threeway(10)First port, the second threeway(10)Second port in be provided with pressure sensor(6), second
Threeway(10)The 3rd port be connected to and artificial blood vessel's sample by pipeline(13)One end connection the first joint(11).
5. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 4, it is characterised in that described pipeline
For silicone tube.
6. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterised in that described the 3rd
Tongs is provided with pipeline(9).
7. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterised in that described frequency conversion
The pump water of pump is controllable, so as to control hydraulic pressure in 0-30kPa scopes, control accuracy is ± 0.1kP a.
8. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterised in that described pulsation
Formula artificial blood vessel entirety water permeability tester also includes metering scales, and described metering scales collect artificial blood vessel's sample
(13)The liquid that oozes out simultaneously carries out continuous metering.
9. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 8, it is characterised in that described metering
Balance is prevented because pressure oscillation causes the upper part that liquid bursts out away and can be by including can artificial blood vessel be protected
The funnel shaped bottom that the liquid oozed out is collected and measured is grouped into, and upper and lower two parts are integrally formed or linked together, metering
The bottom of the lower part of balance is measurement zone.
10. the application method of the overall water permeability tester of pulsating artificial blood vessel any one of claim 1-9, its
It is characterised by, including:
Step 1:Editor needs the pulsating waveform being carried in artificial blood vessel's sample in module occurs for pulsation, sets expected arteries and veins
Dynamic pressure scope;
Step 2:In water tank(1)Artificial blood vessel's sample two ends are separately fixed at the first joint with suture by middle water filling(11)With
Second joint(12)On;
Step 3:Open variable frequency pump(3)Water supply start, adjusts tongs(9)And the motion of controlled level motion motor, until pressure
Force snesor(6)The pressure limit measured is equal to expected fluctuation pressure scope;Metering scales are placed in artificial blood vessel's sample
Lower section, the liquid volume read in metering scales for every 60 seconds, after 10-60 minutes, is calculated under the pressure, sample every square centimeter is every
The overall water permeability of the average value and standard deviation, i.e. artificial blood vessel's sample of the water percolating capacity of minute;
Step 4:Close variable frequency pump(3), outward winding tongs(9), unload artificial blood vessel's sample(13), it is completed.
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WO2018010625A1 (en) * | 2016-07-11 | 2018-01-18 | 苏州迈迪威检测技术有限公司 | Blood flow path simulation system |
CN107328700A (en) * | 2017-07-06 | 2017-11-07 | 南通纺织丝绸产业技术研究院 | A kind of multifunctional artificial Vascular permeability performance test device |
CN107271348B (en) * | 2017-07-06 | 2019-11-15 | 苏州大学 | A kind of medical artificial pipeline permeance property test macro and its application method |
CN107748128A (en) * | 2017-11-27 | 2018-03-02 | 郑州大学 | The biomechanical property test device and method of a kind of scaffold for vascular tissue engineering |
CN108061698A (en) * | 2017-12-11 | 2018-05-22 | 叶剑红 | The full-automatic experimental rig and its method of measurement fluctuation current infiltration towing force coefficient |
CN108051141A (en) * | 2017-12-29 | 2018-05-18 | 四川大学华西医院 | A kind of imitative body of adjustable-pressure ultrasound |
CN109470822B (en) * | 2018-10-24 | 2024-02-06 | 苏州大学 | Device for testing blocking performance of aortic trauma hemostatic material and instrument and evaluation method thereof |
CN110823782A (en) * | 2019-11-25 | 2020-02-21 | 上海畅迪医疗科技有限公司 | Polymer pipe osmotic pressure detection device and application method thereof |
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CN1186615C (en) * | 2003-06-12 | 2005-01-26 | 东华大学 | Textile type artificial blood vessel water permeability test equipment and its testing method |
CN101000336B (en) * | 2007-01-11 | 2010-06-30 | 上海交通大学 | Dynamic investigating method for performance of reverse osmosis antisludging agent |
EP2253735B1 (en) * | 2009-05-13 | 2017-11-22 | SiO2 Medical Products, Inc. | Vessel processing |
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