CN105021509A - Pulsing-type artificial blood vessel overall water permeability tester and using method thereof - Google Patents
Pulsing-type artificial blood vessel overall water permeability tester and using method thereof Download PDFInfo
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- CN105021509A CN105021509A CN201510418675.2A CN201510418675A CN105021509A CN 105021509 A CN105021509 A CN 105021509A CN 201510418675 A CN201510418675 A CN 201510418675A CN 105021509 A CN105021509 A CN 105021509A
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Abstract
The invention provides a pulsing-type artificial blood vessel overall water permeability tester and a using method thereof. The pulsing-type artificial blood vessel overall water permeability tester is characterized by including a water tank; a water supply port of the water tank is connected with a variable frequency pump through a first pipeline, the variable frequency pump is connected with a first connector for being connected with one end of an artificial blood vessel sample through a second pipeline, and a second connector for being connected with the other end of the artificial blood vessel sample is connected with a return water port of the water tank through a third pipeline, wherein the second pipeline is connected with a pulsing generation module. The tester can perform tests under various waveforms and various pressure sections, well simulates a real transplant environment, and is close to the actual usage condition.
Description
Technical field
The present invention relates to the overall water permeability tester of a kind of pulsating artificial blood vessel and using method thereof, belong to medicine equipment proving installation field.
Background technology
With the development of human society, vascular diseases are the first killers of human health always.The appearance of vasotransplantation and develop into surgical operation therapy vascular diseases and provide a solution.At present, artificial blood vessel is successfully applied to clinical, solves the problems such as the shortage of biological species grafting vessel, rejection, has obtained good social benefit and considerable economic benefit.Specify according to international standard ISO 7198-1998, artificial blood vessel must carry out water permeability test before transplantation, and water permeability must meet clinical requirement can transplant.
At present, test artificial blood vessel's water permeability and have two kinds of modes: water permeability and overall water permeability.In other words, during artificial blood vessel's test water perviousness, can be circular flat sample (corresponding water permeability test), also can be tubulose sample (corresponding overall water permeability test), the water percolating capacity of test 16.0kPa ± 0.3kPa (120mmHg ± 2mmHg) pressure lower unit interval unit area.Obviously, overall water permeability test is more close to the transplanting environment of artificial blood vessel, and result is objective reality more.
But the vascular pressure force value of human body different parts is different, and there is systolic pressure and diastolic pressure, not single force value.Therefore, the pressure that the artificial blood vessel being transplanted to different parts bears also is different.In order to can environment well after analogue simulation synthetic vascular grafts, and more scientifically test its overall water permeability, the present invention proposes the overall water permeability tester of a kind of pulsating artificial blood vessel.Through retrieval, there is no special purpose device or the instrument of the overall water permeability of simulation pulsating condition test artificial blood vessel at present both at home and abroad.
Summary of the invention
Technical matters to be solved by this invention is to provide the overall water permeability tester of a kind of pulsating artificial blood vessel and using method thereof, can regulate pressure arbitrarily on the one hand within the scope of 0-30kPa; On the other hand, the pulsation of certain waveform can be provided.By the innovation of above 2, the transplanting environment of artificial blood vessel can be simulated more truly, have an objective appraisal to the overall water permeability of artificial blood vessel.
In order to solve the problem, the invention provides the overall water permeability tester of a kind of pulsating artificial blood vessel, it is characterized in that, comprise water tank, the feed water inlet of water tank is passed through the first pipeline and is connected variable frequency pump, and variable frequency pump connects the first joint being used for being connected with one end of artificial blood vessel's sample by the second pipeline, and the second joint for being connected with the other end of artificial blood vessel's sample passes through the water return outlet that the 3rd pipeline connects water tank, wherein, there is model calling with pulsation in the second described pipeline.
Preferably, described pulsation generation module comprises tangential movement motor, computer surveillance equipment and metallic bellows, described computer surveillance equipment connects tangential movement motor, tangential movement motor can drive metallic bellows to carry out tangential movement to form pulsating wave under the control of computer surveillance equipment, and metallic bellows connects the second pipeline.
Preferably, be provided with pressure transducer in the second described pipeline, pressure transducer connects pulsation and module occurs, and real-time pressure value can be fed back in pulsation generation module.
Preferably, the second described pipeline comprises the first threeway and the second threeway, first port of the first threeway connects variable frequency pump by pipeline, second port of the first threeway connects pulsation by pipeline and module occurs, 3rd port of the first threeway connects the first port of the second threeway by pipeline, be provided with pressure transducer in second port of the second threeway, the 3rd port of the second threeway connects the first joint being used for being connected with one end of artificial blood vessel's sample by pipeline.
More preferably, described pipeline is silicone tube.
Preferably, the first described pipeline and the 3rd pipeline are silicone tube.
Preferably, tongs is provided with in the 3rd described pipeline.
Preferably, described water tank is the container of the storing liquid of metal or plastic material.
Preferably, described water tank is regular shape or irregularly shaped.
Preferably, the pump water yield of described variable frequency pump is controlled, thus controls hydraulic pressure in 0-30kPa scope, and control accuracy is ± 0.1kPa.
Preferably, the overall water permeability tester of described pulsating artificial blood vessel also comprises metering scales, and described metering scales can be collected liquid that artificial blood vessel's sample oozes out and carry out continuous measurement.
More preferably, described metering scales is made up of transparent organic glass.
More preferably; described metering scales is by artificial blood vessel being protected interior; the funnel shaped lower part composition preventing the upper part because pressure surge causes liquid to enter to be shot out and the liquid collection oozed out also can be measured; upper and lower two parts are one-body molded or link together, and the bottom of the lower part of metering scales is measurement zone.
Present invention also offers the using method of the overall water permeability tester of above-mentioned pulsating artificial blood vessel, it is characterized in that, comprising:
Step 1: in pulsation, module inediting occurring needs to be carried in the pulsating waveform in artificial blood vessel's sample, arranges the fluctuation pressure scope of expection;
Step 2: water filling in water tank, is separately fixed on the first joint and the second joint by artificial blood vessel's sample two ends suture line;
Step 3: open variable frequency pump water supply start, regulates tongs and the motion of level of control motion motor, until the pressure limit that pressure transducer records equals the fluctuation pressure scope of expecting; Metering scales is placed in below artificial blood vessel's sample, within every 60 seconds, read the liquid volume in metering scales, after 10-60 minute, under calculating this pressure, the mean value of the water percolating capacity that every square centimeter of sample is per minute and standard deviation, the i.e. overall water permeability of this artificial blood vessel's sample;
Step 4: close variable frequency pump, tongs of outwarding winding, unloads artificial blood vessel's sample, be completed.
Compared with prior art, the present invention has following beneficial effect:
1. the water tank in the overall water permeability tester of pulsating artificial blood vessel of the present invention is the container of storing liquid, variable frequency pump regulates fluid flow in pipeline, computer surveillance equipment level of control motion motor, metallic bellows is driven to form the ripple of various waveform, real-time pressure value in circulating system feeds back in computer surveillance equipment by pressure transducer, by the pressure regulating variable frequency pump and tongs can obtain any pressure stage, it is a closed circulating system that all modules to be connected with threeway etc. by silicone tube.The liquid oozed out from artificial blood vessel flows in metering scales, directly reads data, calculates artificial blood vessel's water permeability.The present invention can realize testing under various waveform, various pressure stage, simulates well and truly transplants environment, closing to reality service condition;
2. the present invention is applicable to artificial blood vessel's sample of all size, various molding mode.
3. the present invention devises metering scales; described metering scales is made up of upper and lower two parts; described artificial blood vessel's sample is located within the upper part of metering scales; the bottom of metering scales is divided into infundibulate; can protect and collect the liquid oozing out and; in extremely rapid succession can read data simultaneously, avoid the error that replacing water container causes.
Accompanying drawing explanation
Fig. 1 is pulsating artificial blood vessel overall water permeability tester schematic diagram;
Embodiment
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Embodiment 1
As shown in Figure 1, for pulsating artificial blood vessel overall water permeability tester schematic diagram, the overall water permeability tester of described pulsating artificial blood vessel comprises water tank 1, the feed water inlet of water tank 1 connects variable frequency pump 3 by the first pipeline, variable frequency pump 3 connects the first joint 11 being used for being connected with one end of artificial blood vessel's sample 13 by the second pipeline, the second joint 12 for being connected with the other end of artificial blood vessel's sample 13 passes through the water return outlet that the 3rd pipeline connects water tank 1, wherein, there is model calling with pulsation in the second described pipeline.Described pulsation generation module is made up of tangential movement motor 4, computer surveillance equipment 7 and metallic bellows 5, described computer surveillance equipment 7 connects tangential movement motor 4, tangential movement motor 4 can drive metallic bellows 5 to carry out tangential movement to form pulsating wave under the control of computer surveillance equipment 7, and metallic bellows 5 connects the second pipeline.
The second described pipeline comprises the first threeway 14 and the second threeway 10, first port of the first threeway 14 connects variable frequency pump 3 by silicone tube, second port of the first threeway 14 connects by silicone tube the metallic bellows 5 that module occurs in pulsation, 3rd port of the first threeway 14 connects the first port of the second threeway 10 by silicone tube, pressure transducer 6 is provided with in second port of the second threeway 10, pressure transducer 6 connects the computer surveillance equipment 7 that module occurs in pulsation, and real-time pressure value can be fed back in the computer surveillance equipment 7 of pulsation generation module.3rd port of the second threeway 10 connects the first joint 11 being used for being connected with one end of artificial blood vessel's sample 13 by silicone tube.The first described pipeline and the 3rd pipeline are silicone tube.Tongs 9 is provided with in the 3rd described pipeline.By the pressure regulating variable frequency pump 3 and tongs 9 can obtain any pressure stage.
Described water tank is the container of the storing liquid of columniform plastic material; The pump water yield of described variable frequency pump is controlled, thus controls hydraulic pressure in 0-30kPa scope, and control accuracy is ± 0.1kPa.The overall water permeability tester of described pulsating artificial blood vessel also comprises metering scales, and the liquid oozed out from artificial blood vessel flows in metering scales, directly reads data, calculates artificial blood vessel's water permeability.Described metering scales is made up of transparent organic glass; by artificial blood vessel being protected interior; the upper part preventing from causing liquid to enter to be shot out due to pressure surge and the liquid collection oozed out can be carried out the funnel shaped lower part composition of continuous measurement; upper and lower two parts are one-body molded, and the bottom of the lower part of metering scales is measurement zone.
Utilize the overall water permeability tester of above-mentioned pulsating artificial blood vessel, under test 7-12kPa condition, pulsating waveform be sine wave, interior diameter be 6mm, long 70mm the operation steps of overall water permeability of artificial blood vessel's sample as follows:
Step 1: need the pulsating waveform-sinusoidal waveform be carried in artificial blood vessel's sample 13 at computer surveillance equipment 7 inediting of pulsation generation module, the fluctuation pressure scope arranging expection is 7-12kPa;
Step 2: fill clean, that pass through filtration, under room temperature water in water tank 1, get artificial blood vessel's sample (diameter 6mm, long 70mm), artificial blood vessel's sample 13 two ends suture line is separately fixed on the first joint 11 and the second joint 12, makes whole pipeline form loop;
Step 3: open variable frequency pump 3 water supply start, observes the force value that pressure transducer 6 records, and regulates tongs 9, makes force value rise to 9.5kPa.Level of control motion motor simultaneously, make metallic bellows 5 do sinusoidal motion, metallic bellows 5 does motion in one dimension, and direction of motion is parallel to the axis of metallic bellows 5, observes the pressure limit that pressure transducer 6 records, until reach 7-12kPa.Metering scales to be placed in below artificial blood vessel's sample and to start timing, within every 60 seconds, reading the liquid volume in metering scales.After 10 minutes, under calculating this pressure stage, the mean value of the amount of seepage that every square centimeter of sample is per minute and standard deviation, the i.e. overall water permeability of this artificial blood vessel's sample;
Step 4: close variable frequency pump 3, tongs 9 of outwarding winding, unloads artificial blood vessel's sample 13, be completed.
As calculated, the overall water permeability of this artificial blood vessel's sample is 70 ± 6.80mL/mincm
2.
Embodiment 2
Utilize the overall water permeability tester of pulsating artificial blood vessel described in embodiment 1, under test 11-16kPa condition, waveform is human body pulsating wave, interior diameter be 20mm, long 210mm the operation steps of overall water permeability of artificial blood vessel's sample as follows:
Step 1: need the pulsating waveform-human pulse waveform be carried in artificial blood vessel's sample 13 at computer surveillance equipment 7 inediting of pulsation generation module, the fluctuation pressure scope arranging expection is 11-16kPa;
Step 2: fill clean, that pass through filtration, under room temperature water in water tank 1, get artificial blood vessel's sample (diameter 20mm, long 210mm), artificial blood vessel's sample 13 two ends suture line is separately fixed on the first joint 11 and the second joint 12, makes whole pipeline form loop;
Step 3: open variable frequency pump 3 water supply start, observes the force value that pressure transducer 6 records, and regulates tongs 9, makes force value rise to 13.5kPa.Level of control motion motor simultaneously, make metallic bellows 5 do pulse wave motion, metallic bellows 5 does motion in one dimension, and direction of motion is parallel to the axis of metallic bellows 5, observes the pressure limit that pressure transducer 6 records, until reach 11-16kPa.Metering scales to be placed in below artificial blood vessel's sample and to start timing, within every 60 seconds, reading the liquid volume in metering scales.After 10 minutes, under calculating this pressure stage, the mean value of the amount of seepage that every square centimeter of sample is per minute and standard deviation, the i.e. overall water permeability of this artificial blood vessel's sample;
Step 4: close variable frequency pump 3, tongs 9 of outwarding winding, unloads artificial blood vessel's sample 13, be completed.
As calculated, the overall water permeability of this artificial blood vessel's sample is 30 ± 4.54mL/mincm
2.
Embodiment 3
Utilize the overall water permeability tester of pulsating artificial blood vessel described in embodiment 1, under test 13-24kPa condition, waveform is square wave, interior diameter be 10mm, long 110mm the operation steps of overall water permeability of artificial blood vessel's sample as follows:
Step 1: need the pulsating waveform-square wave be carried in artificial blood vessel's sample 13 at computer surveillance equipment 7 inediting of pulsation generation module, the fluctuation pressure scope arranging expection is 13-24kPa;
Step 2: fill clean, that pass through filtration, under room temperature water in water tank 1, get artificial blood vessel's sample (diameter 10mm, long 110mm), artificial blood vessel's sample 13 two ends suture line is separately fixed on the first joint 11 and the second joint 12, makes whole pipeline form loop;
Step 3: open variable frequency pump 3 water supply start, observes the force value that pressure transducer 6 records, and regulates tongs 9, makes force value rise to 18.5kPa.Level of control motion motor simultaneously, make metallic bellows 5 do square wave motion, metallic bellows 5 does motion in one dimension, and direction of motion is parallel to the axis of metallic bellows 5, observes the pressure limit that pressure transducer 6 records, until reach 13-24kPa.Metering scales to be placed in below artificial blood vessel's sample and to start timing, within every 60 seconds, reading the liquid volume in metering scales.After 10 minutes, under calculating this pressure stage, the mean value of the amount of seepage that every square centimeter of sample is per minute and standard deviation, the i.e. overall water permeability of this artificial blood vessel's sample;
Step 4: close variable frequency pump 3, tongs 9 of outwarding winding, unloads artificial blood vessel's sample 13, be completed.
As calculated, the overall water permeability of this artificial blood vessel's sample is 280 ± 15.44mL/mincm
2.
Claims (10)
1. the overall water permeability tester of pulsating artificial blood vessel, it is characterized in that, comprise water tank (1), the feed water inlet of water tank (1) connects variable frequency pump (3) by the first pipeline, variable frequency pump (3) connects the first joint (11) being used for being connected with one end of artificial blood vessel's sample (13) by the second pipeline, connected the water return outlet of water tank (1) by the 3rd pipeline for the second joint (12) of being connected with the other end of artificial blood vessel's sample (13), wherein, there is model calling with pulsation in the second described pipeline.
2. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterized in that, described pulsation generation module comprises tangential movement motor (4), computer surveillance equipment (7) and metallic bellows (5), described computer surveillance equipment (7) connects tangential movement motor (4), tangential movement motor (4) can drive metallic bellows (5) to carry out tangential movement to form pulsating wave under the control of computer surveillance equipment (7), and metallic bellows (5) connects the second pipeline.
3. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterized in that, pressure transducer (6) is provided with in the second described pipeline, pressure transducer (6) connects pulsation and module occurs, and real-time pressure value can be fed back in pulsation generation module.
4. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterized in that, the second described pipeline comprises the first threeway (14) and the second threeway (10), first port of the first threeway (14) connects variable frequency pump (3) by pipeline, second port of the first threeway (14) connects pulsation by pipeline and module occurs, 3rd port of the first threeway (14) connects the first port of the second threeway (10) by pipeline, pressure transducer (6) is provided with in second port of the second threeway (10), 3rd port of the second threeway (10) connects the first joint (11) being used for being connected with one end of artificial blood vessel's sample (13) by pipeline.
5. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 4, it is characterized in that, described pipeline is silicone tube.
6. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, is characterized in that, be provided with tongs (9) in the 3rd described pipeline.
7. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterized in that, the pump water yield of described variable frequency pump is controlled, thus controls hydraulic pressure in 0-30kPa scope, and control accuracy is ± 0.1kPa.
8. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 1, it is characterized in that, the overall water permeability tester of described pulsating artificial blood vessel also comprises metering scales, and described metering scales can be collected liquid that artificial blood vessel's sample (13) oozes out and carry out continuous measurement.
9. the overall water permeability tester of pulsating artificial blood vessel as claimed in claim 8; it is characterized in that; described metering scales is by artificial blood vessel being protected interior; the funnel shaped lower part composition preventing the upper part because pressure surge causes liquid to enter to be shot out and the liquid collection oozed out also can be measured; upper and lower two parts are one-body molded or link together, and the bottom of the lower part of metering scales is measurement zone.
10. present invention also offers the using method of the overall water permeability tester of pulsating artificial blood vessel according to any one of claim 1-9, it is characterized in that, comprising:
Step 1: in pulsation, module inediting occurring needs to be carried in the pulsating waveform in artificial blood vessel's sample, arranges the fluctuation pressure scope of expection;
Step 2: water filling in water tank (1), is separately fixed at artificial blood vessel's sample two ends suture line on the first joint (11) and the second joint (12);
Step 3: open variable frequency pump (3) water supply start, regulates tongs (9) and the motion of level of control motion motor, until the pressure limit that pressure transducer (6) records equals the fluctuation pressure scope of expecting; Metering scales is placed in below artificial blood vessel's sample, within every 60 seconds, read the liquid volume in metering scales, after 10-60 minute, under calculating this pressure, the mean value of the water percolating capacity that every square centimeter of sample is per minute and standard deviation, the i.e. overall water permeability of this artificial blood vessel's sample;
Step 4: close variable frequency pump (3), tongs of outwarding winding (9), unloads artificial blood vessel's sample (13), be completed.
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| CN107328700A (en) * | 2017-07-06 | 2017-11-07 | 南通纺织丝绸产业技术研究院 | A kind of multifunctional artificial Vascular permeability performance test device |
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| CN107748128A (en) * | 2017-11-27 | 2018-03-02 | 郑州大学 | The biomechanical property test device and method of a kind of scaffold for vascular tissue engineering |
| CN108051141A (en) * | 2017-12-29 | 2018-05-18 | 四川大学华西医院 | A kind of imitative body of adjustable-pressure ultrasound |
| CN108061698A (en) * | 2017-12-11 | 2018-05-22 | 叶剑红 | The full-automatic experimental rig and its method of measurement fluctuation current infiltration towing force coefficient |
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| CN109470822A (en) * | 2018-10-24 | 2019-03-15 | 苏州大学 | A kind of test device and its evaluation method of main artery wound hemostasis material and instrument sealing characteristics |
| CN109470822B (en) * | 2018-10-24 | 2024-02-06 | 苏州大学 | Device for testing blocking performance of aortic trauma hemostatic material and instrument and evaluation method thereof |
| CN110823782A (en) * | 2019-11-25 | 2020-02-21 | 上海畅迪医疗科技有限公司 | Polymer pipe osmotic pressure detection device and application method thereof |
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