CN105010186B - The method of sponge young directional induction settlement and metamorphosis - Google Patents
The method of sponge young directional induction settlement and metamorphosis Download PDFInfo
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- CN105010186B CN105010186B CN201510413993.XA CN201510413993A CN105010186B CN 105010186 B CN105010186 B CN 105010186B CN 201510413993 A CN201510413993 A CN 201510413993A CN 105010186 B CN105010186 B CN 105010186B
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- young
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The method of sponge young directional induction settlement and metamorphosis, is related to spongia.Comprise the following steps：1) sponge floating larva；2) young is seated in light transmission container；3) attachment base is selected, is immersed in before directional induction attachment in natural sea area；4) placed inside the container of sponge young attachment and make unrestrained pump, make to make unrestrained pump caused by current be internally formed current in container；5) adhesion amount of the sponge young in attachment base is detected, takes out the high attachment base of attachment density, for the young body culture in later stage, increases the treated attachment base of respective numbers.Directional induction especially suitable for blade mountain and sea silk floss floating larva is adhered to.
Description
Technical field
The present invention relates to spongia, more particularly, to a kind of method of sponge young directional induction settlement and metamorphosis.
Background technology
Spongia form of diverse, species is various, widely distributed, from fresh water to seawater, there is sea from intertidal zone to deep-sea
The trace of continuous animal.Because sponge and its microorganism of symbiosis can produce many novel antibacterials, anti-inflammatory, antitumor, anti-
The compound of the difference in functionalitys such as virus, and heat very few in nature content, therefore falling over each other to cultivate as various countries' researcher
Point.The generative propagation for carrying out spongia is to carry out the most convenient method of spongia scale propagation.
The set life of spongia adult battalion, attachment base are generally solid matter, such as stone, coral, shell, timber etc.
The surface of solid.The young leaves the pelagic period for having 0~15 day after parent.The cold water species at some deep-seas, its pelagic period are
Several months.What these floating larvas had active to environment finds out ability, can be chosen whether to be adhered to according to the characteristics of environment,
It is abnormal.Seminar finds that the continuous floating larva of mountain and sea has photophobism in attaching process by long-term research, observation；The young
It is more likely to be attached to the coarse surface of solids；0.5~1m/s current can promote the quick attachment of the sponge young.
The content of the invention
It is an object of the invention to provide a kind of method of sponge young directional induction settlement and metamorphosis.
The present invention comprises the following steps：
1) sponge floating larva；
2) young is seated in light transmission container；
3) attachment base is selected, is immersed in before directional induction attachment in natural sea area；
4) placed inside the container of sponge young attachment and make unrestrained pump, make to make unrestrained pump caused by current be internally formed in container
Current；
5) adhesion amount of the sponge young in attachment base is detected, takes out the high attachment base of attachment density, the young body for the later stage
Culture, increase the treated attachment base of respective numbers.
In step 1), the specific method of the floating larva that sponges can be：
In sponge adult of the natural waterses selection apposition growth on cultivation rope or fishing net；In the water outlet of spongia
Osculum is nearby cut in hole, observes the fish brood amount of apopore base portion sponge parent；Select embryo's content big, and develop more ripe sea
Continuous parent；Prepare a young collecting net, etting selects the screen cloth more smaller than sponge embryo's diameter, and young collecting net is in handstand wine
Ampuliform, young receiving tank is connect from the smaller etting of mesh, bottleneck in bottleneck portion；After the sun sets, above sponge parent
If overcover；The overcover above sponge parent is removed before sunrise, while young collecting net is enclosed on whole sponge parent
It is outside；Sponge parent is moved on to the cultivation of nature marine site after sunrise from young collecting net, waits and continues within second day to collect the young；
Lift young collecting net, seawater filters out naturally, allows the young to focus in the collecting tank of bottom, removes collecting tank, the young is moved to
In cultivation attachment container, subsequent operation is waited；
The length of the osculum can be 2~3cm；The more ripe mark of the development can be：If spongia is blade mountain
Sponge, embryo are in lavender；If spongia is asthma sphagna sponge, embryo is in bright orange；If spongia is simple bone sponge mesh
Class, occurs black ciliary ring at the top of embryo；A diameter of 500~800 μm of the sponge embryo, the screen cloth can use 60 mesh
Nylon screen；The smaller etting of the mesh can use 100 mesh ettings；The young receiving tank can use the children of convenient dismounting
Body receiving tank；The overcover can use black cloth or plank etc., to reduce diffused light, and avoid disturbing；It is described to be incited somebody to action before sunrise
Overcover above sponge parent removes, and 1~2h removes the overcover above sponge parent preferably before sunrise；It is described in day
Sponge parent is moved on to from young collecting net to the cultivation of nature marine site, by sponge parent from children preferably after 1~2h of sunrise after going out
The cultivation of nature marine site is moved on in body collecting net.
In step 2), the density of the young can be 20~100ind/L, to substantially reduce the young on the wall
Adhere to quantity.
In step 3), the attachment base can use rough surface, the deeper natural or artificial solid attachment base of color, such as
Cultivation rope, breeding net, bunchiness oyster shell, artificial PVC board etc., it is described to be immersed in natural sea area before directional induction attachment
In, it can be immersed in before directional induction attachment in natural sea area within 3~7 days, it is therefore an objective to which the harmful substance allowed in these attachment bases is molten
Solution, dissipate, while adhering to one layer of microorganism of primary surface spontaneous growth, the attachment speed of the young can be dramatically speeded up.
In step 4), it is described make unrestrained pump and can be selected commercially available make unrestrained pump commodity；The most strong part of the flow velocity of the current is best
Opposite container inner wall, the purpose is to allow the young to be attached in attachment base, rather than container inner wall, because current can promote the young
Attachment, but make that flow velocity caused by stream pump is uneven, allows the part impact container inner wall that flow velocity is most strong, can not only be by flow velocity point
Dissipate, uniformly, while the young on chamber wall can be washed away.
In step 5), the adhesion amount of the sponge young preferably detects once every 4~6h in the detection attachment base, sponge
The young is attached with effect of bunching, and constantly removes the high attachment base of attachment density, can ensure that overall attachment even density.
The present invention is directed to the above feature embodied in sponge young attaching process, provides a set of effective orientation
The method for inducing sponge floating larva settlement and metamorphosis, this method are attached especially suitable for the directional induction of blade mountain and sea silk floss floating larva
.
Embodiment
The embodiment of the present invention comprises the following steps：
1) sponge floating larva：Natural waterses selection apposition growth cultivation rope or fishing net on sponge into
Body；Osculum is cut near the apopore of spongia, observes the fish brood amount of apopore base portion sponge parent；Select embryo's content
Greatly, and the more ripe sponge parent of development；Prepare a young collecting net, etting selects the sieve more smaller than sponge embryo's diameter
Net, young collecting net are in handstand bottle shape, and young receiving tank is connect from the smaller etting of mesh, bottleneck in bottleneck portion；The sun
After setting, overcover is set above sponge parent；The overcover above sponge parent is removed before sunrise, while the young is received
Collect net outside whole sponge parent；Sponge parent is moved on to the cultivation of nature marine site after sunrise from young collecting net, etc.
Treat to continue within second day to collect the young；Lifting young collecting net, seawater filters out naturally, allows the young to focus in the collecting tank of bottom,
Collecting tank is removed, the young is moved in cultivation attachment container, waits subsequent operation；The length of the osculum can be 2~3cm；Institute
Stating the more ripe mark of development can be：If spongia is that blade mountain and sea is continuous, embryo is in lavender；If spongia is asthma sphagna
Sponge, embryo are in bright orange；If spongia is simple bone sponge mesh class, occurs black ciliary ring at the top of embryo；The sea
Continuous a diameter of 500~800 μm of embryo, the screen cloth can use the nylon screen of 60 mesh；The smaller etting of the mesh can use
100 mesh ettings；The young receiving tank can use the young receiving tank of convenient dismounting；The overcover can use black cloth or plank
Deng to reduce diffused light, and avoiding disturbing；It is described to remove the overcover above sponge parent before sunrise, preferably in sunrise
Preceding 1~2h removes the overcover above sponge parent；It is described to move on to sponge parent certainly from young collecting net after sunrise
Right marine site cultivation, moves on to sponge parent preferably after 1~2h of sunrise the cultivation of nature marine site from young collecting net.
2) young is seated in light transmission container；The density of the young can be 20~100ind/L, to substantially reduce children
The attachment quantity of body on the wall.
3) attachment base is selected, is immersed in before directional induction attachment in natural sea area；The attachment base can use surface thick
It is rough, the deeper natural or artificial solid attachment base of color, such as cultivation rope, breeding net, bunchiness oyster shell, artificial PVC board
Deng, it is described to be immersed in before directional induction attachment in natural sea area, natural sea can be immersed within 3~7 days before directional induction attachment
Qu Zhong, it is therefore an objective to the harmful substance allowed in these attachment bases dissolves, dissipated, while in attachment one layer of micro- life of primary surface spontaneous growth
Thing, the attachment speed of the young can be dramatically speeded up.
4) placed inside the container of sponge young attachment and make unrestrained pump, make to make unrestrained pump caused by current be internally formed in container
Current；It is described make unrestrained pump and can be selected commercially available make unrestrained pump commodity；The preferably opposite container inner wall in the most strong part of the flow velocity of the current, its
Purpose is to allow the young to be attached in attachment base, rather than container inner wall, because current can promote the young to adhere to, but makes stream pump
Caused flow velocity is uneven, allows the most strong part of flow velocity to impact container inner wall, can not only be scattered, uniform by flow velocity, while can incite somebody to action
The young on chamber wall is washed away.
5) adhesion amount of the sponge young in attachment base is detected, takes out the high attachment base of attachment density, the young body for the later stage
Culture, increase the treated attachment base of respective numbers.In the detection attachment base adhesion amount of the sponge young preferably every 4~
6h is detected once, and the sponge young is attached with effect of bunching, and constantly removes the high attachment base of attachment density, can ensure that overall attached
Even density.
Claims (7)
1. the method for sponge young directional induction settlement and metamorphosis, it is characterised in that comprise the following steps：
1) sponge floating larva, and specific method is：In natural waterses selection apposition growth on cultivation rope or fishing net
Sponge adult；Osculum is cut near the apopore of sponge adult, observes the fish brood amount of apopore base portion sponge parent；Selection
Embryo's content is big, and develops more ripe sponge parent；Prepare a young collecting net, etting is selected to be omited than sponge embryo diameter
Small screen cloth, young collecting net are in handstand bottle shape, and in bottleneck portion from the smaller etting of mesh, bottleneck connects young reception
Tank；After the sun sets, overcover is set above sponge parent；The overcover above sponge parent is removed before sunrise, simultaneously
Young collecting net is enclosed on outside whole sponge parent；Sponge parent is moved on into nature marine site from young collecting net after sunrise
Cultivation, wait and continue within second day to collect the young；Lift young collecting net, seawater filters out naturally, allows the young to focus on the children of bottom
In body receiving tank, young receiving tank is removed, the young is moved in cultivation attachment container, waits subsequent operation；
The development is more ripe to be masked as：If spongia is that blade mountain and sea is continuous, embryo is in lavender；If spongia is asthma
Sphagna sponge, embryo are in bright orange；If spongia is simple bone sponge mesh class, occurs black ciliary ring at the top of embryo；Institute
A diameter of 500~800 μm of sponge embryo is stated, the screen cloth uses the nylon screen of 60 mesh；The smaller etting of the mesh uses
100 mesh ettings；
The young receiving tank is the young receiving tank using convenient dismounting；The overcover uses black cloth or plank；It is described
The overcover above sponge parent is removed before sunrise, is that 1~2h removes the overcover above sponge parent before sunrise；Institute
State and sponge parent is moved on to the cultivation of nature marine site after sunrise from young collecting net, be by sponge parent after 1~2h of sunrise
The cultivation of nature marine site is moved on to from young collecting net；
2) young is seated in light transmission container；
3) attachment base is selected, is immersed in before directional induction attachment in natural sea area；
4) placed inside the container of sponge young attachment and make unrestrained pump, make to make unrestrained pump caused by current be internally formed water in container
Stream；
5) adhesion amount of the sponge young in attachment base is detected, takes out the high attachment base of attachment density, for the young body culture in later stage,
Increase the treated attachment base of respective numbers.
2. the method for sponge young directional induction settlement and metamorphosis as claimed in claim 1, it is characterised in that the length of the osculum
For 2~3cm.
3. the method for sponge young directional induction settlement and metamorphosis as claimed in claim 1, it is characterised in that described in step 2)
The density of the young is 20~100ind/L.
4. the method for sponge young directional induction settlement and metamorphosis as claimed in claim 1, it is characterised in that described in step 3)
Attachment base is restricted using the deeper natural or artificial solid attachment base of rough surface, color, the solid attachment base selected from cultivation
Rope, breeding net, bunchiness oyster shell or artificial PVC board.
5. the method for sponge young directional induction settlement and metamorphosis as claimed in claim 1, it is characterised in that described in step 3)
It is immersed in before directional induction attachment in natural sea area, is to be immersed in before directional induction attachment in natural sea area within 3~7 days.
6. the method for sponge young directional induction settlement and metamorphosis as claimed in claim 1, it is characterised in that described in step 4)
Make unrestrained pump and make unrestrained pump commodity from commercially available；The most strong opposite container inner wall in part of the flow velocity of the current.
7. the method for sponge young directional induction settlement and metamorphosis as claimed in claim 1, it is characterised in that described in step 5)
The adhesion amount of the sponge young is detected once every 4~6h in detection attachment base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510413993.XA CN105010186B (en) | 2015-07-15 | 2015-07-15 | The method of sponge young directional induction settlement and metamorphosis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510413993.XA CN105010186B (en) | 2015-07-15 | 2015-07-15 | The method of sponge young directional induction settlement and metamorphosis |
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| CN105010186A CN105010186A (en) | 2015-11-04 |
| CN105010186B true CN105010186B (en) | 2018-04-10 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105961243A (en) * | 2016-05-13 | 2016-09-28 | 广东海洋大学 | Coral larva oriented bottom sowing method |
| CN109717109A (en) * | 2019-02-01 | 2019-05-07 | 厦门大学 | A kind of fixed point transplantation method of lichee sponge |
| CN111670846B (en) * | 2020-04-20 | 2021-10-15 | 厦门大学 | In-situ cultivation method for sponge larvae |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101099452B (en) * | 2006-07-05 | 2010-07-21 | 中国科学院大连化学物理研究所 | Laboratory artificial cultivating method for intertidal zone marine sponge |
| CN101209880A (en) * | 2006-12-29 | 2008-07-02 | 中国科学院大连化学物理研究所 | Method for purifying cultivation sewage by 'marine sponge-microalgae' integration system |
| CN101322477B (en) * | 2007-06-13 | 2012-06-27 | 中国科学院大连化学物理研究所 | Method for artificially collecting and cultivating larva of intertidal belt sponge with embryo |
| JP4956345B2 (en) * | 2007-09-26 | 2012-06-20 | 株式会社ダイクレ | Coral aquaculture structure |
| CN101723515A (en) * | 2009-12-07 | 2010-06-09 | 大连水产学院 | Method for strengthening sponges to enrich heavy metal in seawater |
| CN102204515B (en) * | 2011-03-11 | 2013-02-06 | 厦门大学 | Method and device for measuring biomass of cultured sponge |
| CN103098734B (en) * | 2013-02-06 | 2014-12-10 | 大连海洋大学 | Aquatic animal fertilized ovum in-vitro incubation device |
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Granted publication date: 20180410 Termination date: 20190715 |