CN105002089A - Culture system for microalgae energy efficiency optimization and method thereof - Google Patents

Culture system for microalgae energy efficiency optimization and method thereof Download PDF

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CN105002089A
CN105002089A CN201510387151.1A CN201510387151A CN105002089A CN 105002089 A CN105002089 A CN 105002089A CN 201510387151 A CN201510387151 A CN 201510387151A CN 105002089 A CN105002089 A CN 105002089A
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algae
micro
light source
incubator
light
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缪晓玲
李金壕
林健
陈�峰
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Shanghai Jiaotong University
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Shanghai Jiaotong University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02—Percolation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Abstract

A culture system for microalgae energy efficiency optimization and a method thereof are disclosed. The system comprises an incubator and an air-conveying part and an electronic monitoring part which are respectively connected to the incubator, wherein the air-conveying part provides shunt air for the incubator; and the electronic monitoring part is connected to the top of the incubator and outputs control level so as to realize light source adjustment according to needs. The system is simple to operate and is obtained by innovation and improvement based on present equipment; equipment reconstruction cost is low; and the problem of energy control during the microalgae culture process is effectively solved. The system can be applied in finding the optimum light source energy utilization scheme to effectively raise the light source energy utilization level in the microalgae culture process.

Description

The culture systems that micro-algae efficiency is optimized and method
Technical field
What the present invention relates to is a kind of technology of microorganism culturing field, and specifically the culture systems optimized of a kind of micro-algae efficiency and method, be conducive to the application cost reducing micro-algae bioenergy technology.
Background technology
The global warming caused due to fossil oil overexploitation and burning at present has been subject to all circles and has paid close attention to widely.The development and application of biomass energy is the important means addressed this problem.In the field of biomass energy, the comprehensive development and utilization of micro-algae is again one of the most promising developing direction.Microalgae has high photosynthesis efficiency, the speed of growth that fat content is high and higher relative to other energy crops, and growth cycle is short, and producing the features such as land occupation area is few, is the ideal chose of production biofuel.
Micro-algae bioenergy has huge potentiality to be exploited and application prospect, but still faces a lot of industrialization bottleneck.
How to improve micro algae growth efficiency, reduce energy consumption, reduce application cost, be still the challenge faced, and in the artificial culture of micro-algae, the efficiency improving micro-algae utilizes level to be reduce the important directions of production cost.
In traditional culture system, people often only pay close attention to the increase of ultimate yield, and have ignored the change that light source energy consumption produces thereupon, such as people are by increasing the intensity of illumination, find the suitableeest light intensity, thus the yield of biomass increasing micro-algae is (see SuzanaWahidin et al, The influence of light intensity and photoperiod on the growth and lipidcontent of microalgae Nannochloropsis sp.Bioresource Technology, 2013,129).In tradition research, people also can pass through, at maintenance different colours light, under the condition that intensity of illumination is consistent, the change of light source colour, in the hope of promoting yield of biomass, but have ignored the energy consumption difference of Different Light, (see DaeGeun Kim et al, Manipulation of lightwavelength at appropriate growth stage to enhance biomass productivity and fatty acid methylester yield using Chlorella vulgaris, Bioresource Technology, 2014,159).
Through finding the retrieval of prior art, open (bulletin) the day 2015.02.18 of Chinese patent literature CN204162697U, discloses the synchronizing culture device of a kind of micro-algae, it is characterized in that comprising a simple and easy CO 2breather and a controllable light cycle culturing rack.CO 2prepared by the reaction of food grade alum, sodium bicarbonate and water, utilize pneumatic pump to aspirate, pass in liquid nutrient medium after air filter purification.Set up automatic clock switch after common fluorescent lamp is fixing and namely become photoperiod controlled illumination apparatus.But the defect of this technology and deficiency are: 1, conveniently cannot change intensity of illumination, often use the mode increasing or reduce number of light sources, this mode, bothersome effort, also out of true; 2, common fluorescent tube is used in this technology, light and shade cannot regulate, fluorescent tube number cannot do not changed, under keeping substratum and the constant condition of lamp group relative position, realize the adjustment of intensity of illumination, but in the research finding best efficiency scheme, these two preconditions must meet; 3, this technology cannot record and regulate the energy consumption level of light source as required, and this is necessary in efficiency research equally; 4, this technology pneumatic pump cannot be allowed to pump gas flow rate by relatively precise and stable adjustment, the control problem of gas flow rate cannot be solved; 5, this technology cannot follow the fluorescent tube according to needing to change different colours, when cannot realize keeping different colours light light intensity identical, the comparison of the capacity volume variance consumed, because in the art, light intensity is regulated to be by changing relative position, change fluorescent tube number realizes, thus the difference in micro-algae energy absorption that these two factors are caused cannot be excluded.
Chinese patent literature CN103966086, open (bulletin) day 2014.08.06, disclose a kind of interior external classification regulation and control light-source system of controllable light intensity light color adjustable, it is characterized in that the change tread adjustment intensity of illumination according to microalgae density in retort and spectral range, but this technology cannot be got rid of due to the change of lamp strip position on the impact that micro-algae energy of light source utilizes.
Above prior art all cannot realize light intensity and photoperiod Collaborative Control, lower to energy utilization rate, is difficult to the industrial requirement meeting micro-algae volume production.
Summary of the invention
The present invention is directed to the uncared-for problem of effect of the suboptimum light intensity existed in prior art, a kind of culture systems and method of micro-algae efficiency optimization are proposed, by the Novel LED light source that light and shade is adjustable, the conbined usage such as electricity consumption monitoring device and microdisk electrode equipment, solve general culture system and cannot realize the quantitative adjustment of energy of light source and the problem of monitoring, achieve Energy Efficiency Ratio at different conditions comparatively, be and utilize this system discovery under the intensity of illumination of suboptimum, compared to the suitableeest light intensity under equal conditions, micro-algae often has higher energy of light source utilising efficiency and grease yield.
The present invention is achieved by the following technical solutions:
The present invention relates to the culture systems that a kind of micro-algae efficiency is optimized, comprise: incubator and the air delivery portion be attached thereto respectively and electronic monitoring part, wherein: air delivery portion provides shunt air in incubator, electronic monitoring part is connected with the top of incubator and exports control level, realizes light source as required and regulates.
Described incubator comprises: casing and be arranged at its inside light source, cultivate mechanism and sensing mechanism, wherein: light source is connected with electronic monitoring part, sensing mechanism is connected with casing and the intensity signal exported in incubator and temperature information, cultivates mechanism and is connected with air delivery portion.
Described sensing mechanism includes but not limited to thermometer, light intensity meter etc.
Described light source includes but not limited to the LED group etc. that can export different colours wavelength, and under varying experimental conditions, in lamp group, LED lamp tube number is all 7, and fluorescent tube number can be made an amendment, but must keep being consistent at different conditions.
Described cultivation mechanism includes but not limited to culture dish, Erlenmeyer flask etc., and this cultivation mechanism has included nutrient solution or substratum, and described micro-algae.
Described air delivery portion comprises: the pneumatic pump connected successively, barometric damper, gas meter and stream splitter, wherein: multiple output terminals of stream splitter are connected from different incubators respectively,
The millipore filtration for filtration sterilization is provided with between described output terminal and incubator, when pumping into air by pneumatic pump, by rubber tubing, gas is passed through barometric damper, gas meter, barometric damper, gas diverter and millipore filtration, finally pass in the substratum in Erlenmeyer flask, wherein twice adjustment in use valve regulation flow velocity, the classification regulation and control of flow velocity, make air-flow more accurately stable, gas meter measures air velocity, and gas diverter distributes air-flow.
Described electronic monitoring part comprises: the power supply connected successively, timer, dimmer and electricity consumption monitoring device, wherein: the output terminal of electricity consumption monitoring device is connected with the light source on incubator.
By the replacing of LED group, the adjustment of dimmer and the difference of timer are arranged, change the color of light, the energy consumption level (light intensity also changes simultaneously) of light source and the light application time in odd-numbered day respectively, simultaneously by electricity consumption monitoring device monitoring electricity consumption level.
The cultural method that the micro-algae efficiency that the present invention relates to said system is optimized, comprises the following steps:
1) the micro-algae growing to logarithmic phase is inoculated in the Erlenmeyer flask containing substratum or nutrient solution, under then this Erlenmeyer flask of the micro-algae of inoculation being fixed on light source, be placed in cover with cloth avoid in the casing of printing opacity;
2) light intensity meter is arranged at in the plane of source parallel in casing, then using the position that institute's strong maximum position of light-metering is placed as Erlenmeyer flask, thus ensure that the consistence of light source and substratum relative position;
3) by set timer odd-numbered day light application time, regulated the brightness of light source by dimmer, adjustment judges whether electricity consumption reaches predetermined energy consumption level, stops regulating, by light intensity meter record light intensity now after arriving desired location;
4) measure the cultivation of micro-algae under the present circumstances and growth data, make micro algae growth curve, and utilize level according to the grease yield of micro algae growth tracing analysis unit volume and efficiency, thus realize optimization production.
Described cultivation and growth data referred to: by the OD value of the tenth of micro-algae the day, by the typical curve of the conversion of OD value and dry cell weight, draw the yield of biomass of unit volume, be divided by by the light source energy consumption data of 10 days and the yield of biomass increased value in ten days, show that the relative efficiency of micro-algae utilizes level, according to OD in 10 days 650micro algae growth curve is made in value change.
Described grease yield, detects in the following manner and obtains:
I) weigh after freezing for the medium centrifugal containing micro-algae in cultivation, obtain microalgae cell dry weight;
Ii) get 1g step I) in the frustule that obtains or algae powder, add 5mL deionized water, ultrasonic disintegrator grinding 5min, adds the mixing of 3mL chloroform/methanol (2:1) solution, stirred at ambient temperature 20min, then the centrifugal 10min of 8000rpm, takes off a layer organic phase, repeats above-mentioned experiment 2 times, merge all organic phases in the test tube weighed, be placed in 70 DEG C of dry solvents of water-bath Back stroke, then oven drying at low temperatures in loft drier, weighing results deducted test tube net weight and be fat content.
Technique effect
Compared with prior art, present invention achieves the research angle of the coordinated regulation from intensity of illumination and light application time, optimize and promote the utilising efficiency of micro-algae to energy of light source, solve the stability of air-flow, relative fixing, the different colored light sources Energy Efficiency Ratio of Erlenmeyer flask and the light source position series of problems such as comparatively, under having found suboptimum light intensity, micro-algae has higher energy utilization efficiency, reach on the constant basis of micro-algae light source energy consumption, promote growth velocity and fat content, to the application of biofuel, there is obvious value.
The technology of the present invention effect comprises further:
1) in micro-algae aerated culture subsystem, front and back employ two barometric dampers, achieve the classification regulation and control of gas flow rate, and make gas flow-regulating more accurate, gas flow rate is more stable.
2) only can need regulate electronic regulator, namely carry out the adjustment of light intensity, traditional method changes light intensity often by adjustment fluorescent tube number, and by comparison, the present invention is simple to operate, time saving and energy saving.
3) be used in light source energy consumption when fixing, by the Collaborative Control of periodicity of illumination and light intensity, realize when not increasing energy consumption, promoting the object of micro algae biomass.
4) can be used for finding the highest intensity of illumination of efficiency, and traditional culture systems can only find the intensity of illumination that Biomass growth is the highest.
6) can be used for finding the highest periodicity of illumination of efficiency, and traditional culture systems can only find the periodicity of illumination that Biomass growth is the highest.
6) can be used for the light source of different colours under the condition that light intensity is identical, the capacity utilization variance of more micro-algae, and traditional culture systems is owing to cannot ensure the relative position consistence at different conditions in fluorescent tube number, Erlenmeyer flask and lamp group, thus cannot realize Energy Efficiency Ratio compared with function.
Accompanying drawing explanation
Fig. 1 is present system schematic diagram;
Fig. 2 is the typical curve of the conversion of embodiment OD value and dry cell weight;
Fig. 3 is Chlorella pyrenoidesa in embodiment, under the condition that total energy consumption is consistent and energy consumption intensity is identical, when the color of LED light source is different, and the result schematic diagram of growing state;
Fig. 4 be in embodiment Chlorella pyrenoidesa under the condition that energy consumption is identical, when power is different, the result schematic diagram of growing state;
Fig. 5 be in embodiment Chlorella pyrenoidesa under different light intensity condition, the result schematic diagram of growing state;
Fig. 6 is Chlorella pyrenoidesa in embodiment, keeps intensity of illumination identical, when light source colour is different, and the result schematic diagram of growing state;
In figure: 1 pneumatic pump, 2 tracheaes, 3 barometric dampers, 4 gas meters, 5 stream splitters, 6 millipore filtrations, 7 Erlenmeyer flasks, 8 nutrient solutions, 9 light intensity meters, 10 thermometers, 11 casings, 12LED lamp group, 13 wires, 14 electricity consumption monitoring devices, 15 dimmers, 16 timers, 17 power supplys.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Unless otherwise, otherwise all technology used in the present invention and scientific terminology and the routine of those of ordinary skill that the present invention relates to technical field understand and have identical meanings.
Energy consumption intensity: refer to the power consumption of micro-algae light source within the unit time.
Micro-algae: refer to unicellular planktonic algae, is preferably the unicellular planktonic algae of any clarified liq substratum pure culture.
Embodiment 1
Chlorella pyrenoidesa, under the condition that odd-numbered day energy consumption is identical with energy consumption intensity, when the color of LED light source is different, by the comparison of growing state result, finds the light source of the color that efficiency is the highest.
1) preparation of substratum: the BG after improving with distilled water preparation ?11 substratum, often liter containing 1.5g NaNO 3, 0.04gK 2hPO 4, 0.075gMgSO 47H 2o, 0.036g CaCl 22H 2the A5 trace element of O, 0.006g citric acid, 0.006g ferric ammonium citrate, 0.001g EDTA and 1.0mL, A5 trace element often rises and contains: 2.86g H 3bO 3, 1.81g MnCl 24H 2o, 0.05g CoCl 26H 2o, 0.79g CuSO 45H 2o, 0.22g ZnSO 47H 2o and 0.39g Na 2moO 4.
2) making of the conversion curve of OD value and dry cell weight:
By the algae liquid of different concns after dilution, measure its OD value;
Accurate absorption 5mL, is transferred in the 10mL centrifuge tube of having weighed.The repetition of three, each sample;
After the centrifugal 10min of 8000rpm, supernatant discarded.Washing with alcohol with 10% twice;
Put into lyophilizer and be dried to constant weight, after precise, calculate dry cell weight;
Repeat aforesaid operations with the algae liquid of different OD value, obtain a series of OD value and corresponding dry cell weight thereof;
With OD value and the mapping of corresponding dry cell weight, obtain conversion curve, as shown in Figure 2.
4) cultivation of Chlorella pyrenoidesa:
Substratum is divided in the ventilation Erlenmeyer flask putting into 1000mL, is respectively charged into 500mL, by the substratum point to install, put into high-pressure sterilizing pot, 120 DEG C, under 30min condition, sterilizing.
In substratum after sterilizing, the algae kind of the Chlorella pyrenoidesa of access proper concn, makes final nutrient solution at OD 650value is 0.1.Will the complete Erlenmeyer flask of inoculation, put into between cloth parcel, with the position that light intensity in light intensity meter determination plane is the highest, Erlenmeyer flask is placed on this position.Erlenmeyer flask is connected with rubber hose, passes into air.Equally, operation does three times, puts into white light source respectively, under blue-light source and red light source.
5) setting of culture condition: odd-numbered day energy consumption is defined as 0.8kwh, and light application time is defined as 18h, thus, the total power calculating light source is 44.4W.Under white light, regulate dimmer, observe electricity consumption monitoring device (can display light source total power) simultaneously, show to electricity consumption monitoring device, when light source total power is 44.4, stop regulating.
Under blue light, regulate dimmer, observe electricity consumption monitoring device (can display light source total power) simultaneously, show to electricity consumption monitoring device, when light source total power is 44.4, stop regulating.
With above two groups similar, under ruddiness, regulate dimmer, observe electricity consumption monitoring device (can display light source total power) simultaneously, show to electricity consumption monitoring device, when light source total power is 44.4, stop regulating.
6) data logging and analysis: get 1mL nutrient solution every day, after dilution, fix on the OD value at 650nm place with the measurement of ultraviolet spectrometry range, use EXCEL to carry out data analysis, experimental result is shown in Fig. 3 and following table.
Note: biomass energy consumption: under certain condition, the total energy consumption of micro-algae 10 days inner light sources, with ten days in the ratio of often liter of biomass increased value.
4) experiment conclusion: under the condition of identical power with identical light application time, the light of different colours consumes identical energy, but creates different growth results.
At odd-numbered day energy consumption 0.8kwh, under the condition of odd-numbered day illumination 18h, the efficiency of blue light is the highest, and white light takes second place, and ruddiness efficiency is the poorest, and wherein ruddiness has exceeded 16% than white light energy consumption, therefore in actual production, with this understanding, should use the white light source of this fluorescent tube kind.
Embodiment 2
Chlorella pyrenoidesa, under the precondition that odd-numbered day energy consumption is identical, under blue light, by the coordinated regulation (namely energy consumption intensity different) of intensity of illumination with periodicity of illumination, promotes energy of light source utilising efficiency.
1) preparation of substratum: the BG after improving with distilled water preparation ?11 substratum, often liter containing 1.5g NaNO 3, 0.04gK 2hPO 4, 0.075gMgSO 47H 2o, 0.036g CaCl 22H 2the A5 trace element of O, 0.006g citric acid, 0.006g ferric ammonium citrate, 0.001g EDTA and 1.0mL, A5 trace element often rises and contains: 2.86g H 3bO 3, 1.81g MnCl 24H 2o, 0.05g CoCl 26H 2o, 0.79g CuSO 45H 2o, 0.22g ZnSO 47H 2o and 0.39g Na 2moO 4.
2) cultivation of Chlorella pyrenoidesa:
Substratum is divided in the ventilation Erlenmeyer flask putting into 1000mL, is respectively charged into 500mL, by the substratum point to install, put into high-pressure sterilizing pot, 120 DEG C, under 30min condition, sterilizing.
In substratum after sterilizing, the algae kind of the Chlorella pyrenoidesa of access proper concn, makes final nutrient solution at OD 650value be 0.1 ?0.11.Will the complete Erlenmeyer flask of inoculation, put into between cloth parcel, with the position that light intensity in light intensity meter determination plane is the highest, Erlenmeyer flask is placed on this position.Erlenmeyer flask is connected with rubber hose, passes into air.Same operation does three times, and putting into power is respectively under the light source of 44.4W, 53.3W and 66.6W.
3) setting of culture condition: odd-numbered day energy consumption is defined as 0.8kwh, and light application time is defined as 18h, thus, the power calculating light source is 44.4W.Under blue light, regulate dimmer, observe electricity consumption monitoring device (can display light source total power) simultaneously, show to electricity consumption monitoring device, when light source total power is 44.4, stop regulating, record intensity of illumination now by light intensity meter simultaneously.
Similar, odd-numbered day energy consumption is defined as 0.8kwh, and light application time is decided to be 15h, and the power calculating light source is 53.3W.Under white light, regulate dimmer, observe electricity consumption monitoring device (can display light source total power) simultaneously, show to electricity consumption monitoring device, when light source total power is 53.3, stop regulating, record intensity of illumination now by light intensity meter simultaneously.
Test similar with above two groups, odd-numbered day energy consumption is defined as 0.8kwh, and light application time is decided to be 12h, and the power calculating light source is 66.6W.Under blue light, regulate dimmer, observe electricity consumption monitoring device (can display light source total power) simultaneously, show to electricity consumption monitoring device, when light source total power is 66.6, stop regulating, record intensity of illumination now by light intensity meter simultaneously.
4) data logging and analysis: get 1mL nutrient solution every day, after dilution, fix on the OD value at 650nm place with the measurement of ultraviolet spectrometry range, use EXCEL to carry out data analysis, experimental result is shown in Fig. 4 and following table.
Note: biomass energy consumption: under certain condition, the total energy consumption of micro-algae 10 days inner light sources, with ten days in the ratio of biomass increased value.
5) experiment conclusion: Chlorella pyrenoidesa is when energy consumption is identical, and under different energy consumption intensity, efficiency obvious difference, when energy consumption intensity is 44.4, efficiency of energy utilization is the highest, and when 53.3W, efficiency is taken second place, when 66.6W, efficiency is the poorest, and optimal conditions compares energy-conservation nearly 60% times of lowest term.
Embodiment 3
Chlorella pyrenoidesa is under white light different light intensity condition, and the comparison of Energy harvesting situation, finds the rule that efficiency changes with light intensity.
1) preparation of substratum: the BG after improving with distilled water preparation ?11 substratum, often liter containing 1.5g NaNO 3, 0.04gK 2hPO 4, 0.075gMgSO 47H 2o, 0.036g CaCl 22H 2the A5 trace element of O, 0.006g citric acid, 0.006g ferric ammonium citrate, 0.001g EDTA and 1.0mL, A5 trace element often rises and contains: 2.86g H 3bO 3, 1.81g MnCl 24H 2o, 0.05g CoCl 26H 2o, 0.79g CuSO 45H 2o, 0.22g ZnSO 47H 2o and 0.39g Na 2moO 4.
2) cultivation of Chlorella pyrenoidesa:
Substratum is divided in the ventilation Erlenmeyer flask putting into 1000mL, is respectively charged into 500mL, by the substratum point to install, put into high-pressure sterilizing pot, 120 DEG C, under 30min condition, sterilizing.
In substratum after sterilizing, the algae kind of the Chlorella pyrenoidesa of access proper concn, makes final nutrient solution at OD 650value be 0.1 ?0.11.Will the complete Erlenmeyer flask of inoculation, put into between cloth parcel, with the position that light intensity in light intensity meter determination plane is the highest, Erlenmeyer flask is placed on this position.Erlenmeyer flask is connected with rubber hose, passes into air.Same operation does three times, and putting into light intensity is respectively under the light source of 80 μm of ol/ms, 100 μm of ol/ms and 120 μm ol/ms, and under keeping three kinds of culture condition, Erlenmeyer flask is consistent to the distance of LED group simultaneously.
4) data logging and analysis: get 1mL nutrient solution every day, after dilution, fix on the OD value at 650nm place with the measurement of ultraviolet spectrometry range, use EXCEL to carry out data analysis, experimental result is shown in Fig. 4 and following table.
Light source colour Odd-numbered day light source energy consumption Odd-numbered day light application time Power Intensity of illumination
In vain 0.756kwh 18h 42W 80μmol/m·s
In vain 0.846kwh 18h 47W 90μmol/m·s
In vain 0.972kwh 18h 54W 100μmol/m·s
In vain 1.332kwh 18h 74W 120μmol/m·s
5) experiment conclusion: under white light different illumination intensity, the utilization of energy of light source is compared, when 90 μm of ol/ms, efficiency of energy utilization is the highest, when 100 μm of ol/ms, efficiency is taken second place, and when 120 μm of ol/ms, efficiency is the poorest, the most minimum efficiency of high energy efficiency ratio energy-conservation nearly 70%, obtains the highest grease yield when 100 μm of ol/ms.
Embodiment 4
Chlorella pyrenoidesa, keeps intensity of illumination identical, and when light source colour is different, the results contrast of growing state, finds the application conditions that efficiency is the highest.
1) preparation of substratum: the BG after improving with distilled water preparation ?11 substratum, often liter containing 1.5g NaNO 3, 0.04gK 2hPO 4, 0.075gMgSO 47H 2o, 0.036g CaCl 22H 2the A5 trace element of O, 0.006g citric acid, 0.006g ferric ammonium citrate, 0.001g EDTA and 1.0mL, A5 trace element often rises and contains: 2.86g H 3bO 3, 1.81g MnCl 24H 2o, 0.05g CoCl 26H 2o, 0.79g CuSO 45H 2o, 0.22g ZnSO 47H 2o and 0.39g Na 2moO 4.
2) cultivation of Chlorella pyrenoidesa:
Substratum is divided in the ventilation Erlenmeyer flask putting into 1000mL, is respectively charged into 500mL, by the substratum point to install, put into high-pressure sterilizing pot, 120 DEG C, under 30min condition, sterilizing.
In substratum after sterilizing, the algae kind of the Chlorella pyrenoidesa of access proper concn, makes final nutrient solution at OD 650value be 0.1 ?0.11.Will the complete Erlenmeyer flask of inoculation, put into between cloth parcel, with the position that light intensity in light intensity meter determination plane is the highest, Erlenmeyer flask is placed on this position.Erlenmeyer flask is connected with rubber hose, passes into air.Same operation does twice, and putting into color is respectively under the light source of white and blueness, and under keeping two kinds of culture condition, Erlenmeyer flask is consistent to the distance of LED group simultaneously.
3) setting of culture condition: odd-numbered day energy consumption is defined as 0.8kwh, and light application time is defined as 18h, thus, is placed on light intensity meter in support, in the plane parallel with LED lamp group, and the position that light intensity is the highest.Under white light, regulate dimmer, observe light intensity meter simultaneously, when to show intensity of illumination be 100 μm of ol/ms, stop regulating to light intensity meter.Equally, under blue light, regulate dimmer, observe light intensity meter simultaneously, when to show intensity of illumination be 100 μm of ol/ms, stop regulating to light intensity meter.
4) data logging and analysis: get 1mL nutrient solution every day, after dilution, fix on the OD value at 650nm place with the measurement of ultraviolet spectrometry range, use EXCEL to carry out data analysis, experimental result is shown in Fig. 6 and following table.
Light source colour Odd-numbered day light source energy consumption Odd-numbered day light application time Power Intensity of illumination
In vain 0.99kwh 18h 55W 100μmol/m·s
Blue 1.026kwh 18h 57W 100μmol/m·s
Light source colour Biomass increased value (g/L) in ten days The energy consumption of biomass Fat content (%) Grease yield (g)
In vain 0.81 12.22 35％ 0.28
Blue 0.67 15.31 40％ 0.26
Note: biomass energy consumption: under certain condition, the total energy consumption of micro-algae 10 days inner light sources, with the ratio of ten days biomass increased values.
4) experiment conclusion: when light intensity is 100 μm of ol/ms, the energy utilization efficiency of micro-algae under white light is higher than blue light, now under white light, grows energy-conservation nearly 25%, and grease yield promotes 8% simultaneously.

Claims (9)

1. the culture systems of micro-algae efficiency optimization, it is characterized in that, comprise: incubator and the air delivery portion be attached thereto respectively and electronic monitoring part, wherein: air delivery portion provides shunt air in incubator, electronic monitoring part is connected with the top of incubator and exports control level, realizes light source as required and regulates.
2. the culture systems of micro-algae efficiency optimization according to claim 1, it is characterized in that, described incubator comprises: casing and be arranged at its inside light source, cultivate mechanism and sensing mechanism, wherein: light source is connected with electronic monitoring part, sensing mechanism is connected with casing and the intensity signal exported in incubator and temperature information, cultivates mechanism and is connected with air delivery portion.
3. the culture systems of micro-algae efficiency optimization according to claim 1, it is characterized in that, described air delivery portion comprises: the pneumatic pump connected successively, barometric damper, gas meter and stream splitter, wherein: multiple output terminals of stream splitter are connected from different incubators respectively.
4. the culture systems of micro-algae efficiency optimization according to claim 1, is characterized in that, be provided with the millipore filtration for filtration sterilization between described output terminal and incubator.
5. the culture systems of micro-algae efficiency optimization according to claim 1, it is characterized in that, described electronic monitoring part comprises: the power supply connected successively, timer, dimmer and electricity consumption monitoring device, wherein: the output terminal of electricity consumption monitoring device is connected with the light source on incubator.
6. the cultural method that micro-algae efficiency of system is optimized according to above-mentioned arbitrary claim, is characterized in that, comprise the following steps:
1) the micro-algae growing to logarithmic phase is inoculated in the Erlenmeyer flask containing substratum or nutrient solution, under then this Erlenmeyer flask of the micro-algae of inoculation being fixed on light source, be placed in cover with cloth avoid in the casing of printing opacity;
2) light intensity meter is arranged at in the plane of source parallel in casing, then using the position that institute's strong maximum position of light-metering is placed as Erlenmeyer flask, thus ensure that the consistence of substratum and light source relative position;
3) by set timer odd-numbered day light application time, regulated the brightness of light source by dimmer, adjustment judges whether electricity consumption reaches predetermined energy consumption level, stops regulating, by light intensity meter record light intensity now after arriving desired location;
4) measure the cultivation of micro-algae under the present circumstances and growth data, make micro algae growth curve, and utilize level according to the grease yield of micro algae growth tracing analysis unit volume and efficiency, thus realize optimization production.
7. method according to claim 6, it is characterized in that, described cultivation and growth data referred to: by the OD value of the tenth of micro-algae the day, by the typical curve of the conversion of OD value and dry cell weight, draw the yield of biomass of unit volume, be divided by by the light source energy consumption data of 10 days and the yield of biomass increased value in ten days, show that the relative efficiency of micro-algae utilizes level, according to OD 650value makes micro algae growth curve.
8. method according to claim 6, is characterized in that, described grease yield, detects in the following manner and obtains:
I) weigh after freezing for the medium centrifugal containing micro-algae in cultivation, obtain microalgae cell dry weight;
Ii) get 1g step I) in the frustule that obtains or algae powder, add 5mL deionized water, ultrasonic disintegrator grinding 5min, adds the mixing of 3mL chloroform/methanol (2:1) solution, stirred at ambient temperature 20min, then the centrifugal 10min of 8000rpm, takes off a layer organic phase, repeats above-mentioned experiment 2 times, merge all organic phases in the test tube weighed, be placed in 70 DEG C of dry solvents of water-bath Back stroke, then oven drying at low temperatures in loft drier, weighing results deducted test tube net weight and be fat content.
9. method according to claim 6, is characterized in that, described nutrient solution is often liter and contains 1.5g NaNO 3, 0.04g K 2hPO 4, 0.075gMgSO 47H 2o, 0.036g CaCl 22H 2the A5 trace element of O, 0.006g citric acid, 0.006g ferric ammonium citrate, 0.001g EDTA and 1.0mL, wherein: A5 trace element often rises and contains: 2.86g H 3bO 3, 1.81g MnCl 24H 2o, 0.05g CoCl 26H 2o, 0.79g CuSO 45H 2o, 0.22g ZnSO 47H 2o and 0.39g Na 2moO 4.
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