CN104998085B - A kind of Traditional Chinese medicine compound composition of improving immunocompetence and preparation method thereof - Google Patents
A kind of Traditional Chinese medicine compound composition of improving immunocompetence and preparation method thereof Download PDFInfo
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Abstract
A kind of Traditional Chinese medicine compound composition of improving immunocompetence is mainly prepared by the respective extracting solution of kinds of traditional Chinese medicines after mixing, concentrating, and includes each component of following mass fraction proportioning：Tortoiseshell 25%~36%, deer horn 25%~36%, the stem of noble dendrobium 19%~25%, Radix Codonopsis 5%~8% and the fruit of Chinese wolfberry 10%~17%；First ready raw material is cleaned when preparation, cutting and drying are weighed according to the ratio；Tortoiseshell, deer horn are decocted respectively, after respective merging decoction liquor is filtered, respectively obtain concentrate A, B；Radix Codonopsis, the fruit of Chinese wolfberry are merged and decocted, concentrate C is obtained；Stem of noble dendrobium processing is prepared into Herba Dendrobii extract；Concentrate A, B and C are merged with Herba Dendrobii extract, concentration, dry, crushing is then proceeded to, Traditional Chinese medicine compound composition is made.The product of the present invention has many advantages, such as that qi and blood is taken into account, tonifying both YIN and YANG and can enhance body's immunity.
Description
Technical field
The present invention relates to field of Chinese medicinal composition more particularly to a kind of Traditional Chinese medicine compound compositions of enhancing body's immunity
And preparation method thereof.
Background technology
Immunity is the defense mechanism of human body itself, is the weapon for identifying and eliminating external intrusion foreign matter, is protection body
The firm fort for not perplexed by disease or being invaded and harassed less by disease.Immune function is too low, and body is to external material want resistance, disease
The harmful substances such as poison, bacterium one will gush and arrive, then will produce various diseases.
Chinese medicine thinks, " in healthy tendency deposits, heresy can not be done；Heresy is gathered, and gas must be empty ", it is empty as physically weak, contain the deficiency of vital energy,
The deficiency of blood, the deficiency of Yin, deficiency of yang many aspects.And the physically weak theoretical immune function illustrated with modern pharmacology is closely related.Therefore,
To prevent generation or the treatment disease of disease, then need to take the side such as QI invigorating, blood-nourishing, enriching yin, supporing yang respectively according to different diseases
Method, to enhance the immune function of body.
The Chinese medicine preparation for enhancing body's immunity includes single medicinal material and Chinese medicine compound prescription.Single Chinese medicine preparation can only be controlled
Certain symptoms are made or alleviate, it is difficult to thoroughly alleviate the complicated body of hypoimmunity.And compound Chinese medicinal preparation can pass through monarch
Minister helps the compatibility made, generates synergistic effect, improves curative effect, reduces toxic side effect.But compound Chinese medicinal preparation is mainly needle at present
There is enhancing body immunity such as the square foundation that Sijunzi Tang is tonifying Qi to the prescription of one or two kinds of asthenic symptoms treatment, improves huge
Phagocyte phagocytic activity is mainly used for treating deficiency of spleen-QI and stomach-QI, and the patient of yin asthenia generating intrinsic heat, abdominal fullness and distention should not then use；Siwu Tang
It enriches blood for Chinese medicine the classic prescriptions of regulating blood condition, is particularly suitable for the immunological regulation of blood-deficiency type body, but partial temperature in the prescription, the deficiency of Yin
The patient of interior heat, which takes, easily leads to excessive internal heat, or even aggravates the state of an illness, and the patient to suffer from indiestion takes can cause diarrhea；Six drugs containing rehmanniae
Ball is tonifying yin side, all has certain humidification to mononuclear-macrophage phagocytic function and T, B lymphocyte proliferation, cures mainly kidney yin
The empty or deficiency of liver-yin and kidney-yin, can also use as the adjuvant therapy medicaments of other symptoms caused by asthenia of qi and blood, but because the medicine of prescription is mostly
Drugs for nourishing yin, yang deficiency patient should not use, and the bad patient's use of spleen and stomach function can interfere digestive function etc..
To sum up, it is clear that it is necessary to prepare a kind of negative and positive of qi and blood and mend, and suitable for deficiency-cold in spleen and stomach patient and effect it is reliable
The Chinese medicine composition that can be really used as immunopotentiator.In addition, the Chinese medicine preparation of most of enhancing human body immune functions
Based on traditional powder, decoction, pill, although at low cost, dosage form falls behind, and does not meet modernization of Chinese medicine requirement, effect
Also it has a greatly reduced quality, and results in waste of resources.Therefore, it is necessary to greatly develop the novel form of Chinese medicine composition.
Invention content
The technical problem to be solved by the present invention is to overcome the shortcomings of to mention in background above technology and defect, provide one
Kind of qi and blood takes into account, tonifying both YIN and YANG and the Chinese medicine composition that can enhance body's immunity, correspondingly provide it is a kind of it is easy to operate,
The preparation method of environmentally friendly, economic, efficient, nontoxic aforementioned Chinese medicine composition.
In order to solve the above technical problems, technical solution proposed by the present invention is a kind of Chinese medicine composition of enhancing immune function
Preparation method, the Traditional Chinese medicine compound composition is mainly by the respective extracting solution of kinds of traditional Chinese medicines through mixing, concentration, drying and other steps
After be prepared, aforementioned kinds of traditional Chinese medicines include following mass fraction proportioning each component：
Tortoiseshell 25%~36%
Deer horn 25%~36%
The stem of noble dendrobium 19%~25%
5%~8% He of Radix Codonopsis
The fruit of Chinese wolfberry 10%~17%.
The Chinese medicine composition of the enhancing immune function of the present invention is based primarily upon formula as below principle：
Deer horn：Promoting circulation of blood, detumescence, kidney-nourishing；Its water soluble ingredient, which has, promotes the phagocytic function of mouse macrophage and T lymphs thin
The proliferation of born of the same parents improves the function of the failure of breast cancer MA-737 mouse T lymphocytes；It is low that its polysaccharide constituents can improve immunity
The T cell sum and TH, TS percentage and TH/TS ratios of lower mouse, and can assist and promote the proliferation of mouse lymphocyte.
Tortoiseshell：Enriching yin, blood-nourishing, hemostasis are considered as the key medicine of Yin enriching heath care.Its Aqueous extracts to deficiency of Yin mouse weight mitigate,
Autonomic activities are reduced, hypoxia-bearing capability weakens and thyroid gland, thymus gland, spleen, adrenal atrophy all there is certain inhibition to make
With.
The stem of noble dendrobium：Yin jin is nourished, builds up health, strengthens the spleen and stomach, protect liver protects courage.It is thin that its polysaccharide constituents can enhance abdominal cavity macrophage
Phagocytic activity of the born of the same parents to chicken red blood cell；ICR mouse inbred lines spleens weight, thymic weight is set to increase, antibody cell number showed increased, T is thin
Born of the same parents and B cell proliferation；Promote the mitotic effect of lymphocyte；Promote lymphocyte number purpose to increase, and promotes lymph
Cell secretes certain chemical substances, to achieve the purpose that enhance immunity of organisms.
Radix Codonopsis：Tonifying middle-Jiao and Qi, strengthening spleen and tonifying lung.Radix Codonopsis extract can enhance the phagocytic function of reticuloendothelial system, to carry
The disease resistance of high body.
The fruit of Chinese wolfberry：It is nourishing liver and kidney, benefiting shrewd head.Its polysaccharide constituents can increase the total T cell number of normal mouse spleen and auxiliary
Helping property T cell subgroup (Th) percentage improves lymphocyte transformation rate, and the immune function that can restore Induced by Cyclophosphamide is low
Under, making total T cell number of reduction, Th subgroups percentage, when lymphocyte transformation rate restores to normal or close to normal level；And
The human tonsil lymphocytes breeder reaction activated to phytohemagglutin phytolectin and phorbol exters has apparent facilitation.
The above-mentioned Traditional Chinese medicine compound composition of the present invention carries out having followed " monarch " this cardinal principle when compound compatibility,
Reuse the deer horn of warming kidney and enhancing body, Yijing blood, Tianjing Busuiye medicinal, nourishing yin and nourishing blood tortoiseshell, high tonifying yin sun is altogether with angry blood sperm marrow
Monarch drug in a prescription；Compatibility Radix Codonopsis mends gas among the taste day after tomorrow, fruit of Chinese wolfberry liver and kidney benefiting, replenishing vital essence and blood, to enhance the source of metaplasia qi and blood；The stem of noble dendrobium nourishes
Yin jin strengthens the spleen and stomach, and is ministerial drug, the effect of to help the work(of tortoise deer, play cooperation monarch drug in a prescription.This five kinds of Chinese medicine, which shares, to be had
Effect plays the synergistic function between Chinese medicine, plays tonifying both YIN and YANG, a series of body shapes such as qi and blood is taken into account, mitigates immunity degradation
The effect of condition mends together to reach negative and positive of qi and blood, consolidates the effect of helping member, eliminating evil conditioning.
In above-mentioned preparation method, it is preferred that the tortoiseshell is tortoise plastron, plastron or colla carapacis et plastri testudinis；The deer horn be CORNU CERVIELAPHI,
CORNU CERVI, pilose antler, cornu cerve degelatinatum, deer horn glue or deer horn pallet；The stem of noble dendrobium is HERBA DENDROBII, Dendrobidium huoshanness, dendrobium candidum, horse
The whip stem of noble dendrobium, Dendrobium Chrysotoxum Lindl, Dendrobium denneanum, Dendrobium chrysanthum, Dendrobium fimbriatum Hook or Dendrobium loddigesii.
In above-mentioned preparation method, it is preferred that the Chinese medicine composition is granule, tablet (including conventional tablet, candy
Lozenge, chewable tablets etc.), capsule (including soft capsule, hard capsule) or oral solution.
The technical concept total as one, the present invention also provides a kind of preparation method of above-mentioned Traditional Chinese medicine compound composition,
Include the following steps：
Ready raw material tortoiseshell, deer horn, the stem of noble dendrobium, Radix Codonopsis, the fruit of Chinese wolfberry are cleaned, cutting and drying, by corresponding quality
Score proportioning is weighed；
The tortoiseshell, deer horn are put into extraction tank respectively, added water to cook, after respective merging decoction liquor is filtered, point
Concentrate A and concentrate B are not obtained；
The Radix Codonopsis, fruit of Chinese wolfberry merging are put into extraction tank, is added water to cook, is filtered after merging decoction liquor, obtain concentrate
C；
Raw material stem of noble dendrobium processing is prepared into Herba Dendrobii extract；
Concentrate A obtained above, concentrate B, concentrate C are merged with Herba Dendrobii extract, concentration is then proceeded to, does
Dry, crushing, and it is suitably added auxiliary material as needed, obtain the Traditional Chinese medicine compound composition.
The effect of in above-mentioned preparation method particular for each bulk pharmaceutical chemicals and processing characteristics carry out technological operation step
It improves, since tortoiseshell, deer horn are animal drugs, active material therein can be effectively extracted by decocting long；And Radix Codonopsis, matrimony vine with
These autonomic drugs of the stem of noble dendrobium then can be dissolved out its active ingredient by conventional decoction；And it is fully extracted in order to more therein
Active ingredient, it has been found that the extraction time of deer horn is longer with respect to tortoiseshell, so especially having adjusted different component in the present invention
Extraction scheme is decocted, and such scheme is also more energy saving.
In above-mentioned preparation method, it is preferred that adding water to cook in the step is 2-4 times, when decoction tortoiseshell or deer horn
When, the time decocted every time is 16-34h；When decocting Radix Codonopsis, the fruit of Chinese wolfberry, the time decocted every time is 1.0-2.5h.More preferably
, in this step, when decocting deer horn, the number that adds water to cook is 3 times, the time decocted every time be respectively 26-34h, 26-34h,
16-24h；When decocting tortoiseshell, the number added water to cook is 3 times, and the time decocted every time is 16-24h；Decoct Radix Codonopsis and matrimony vine
The period of the day from 11 p.m. to 1 a.m, the number added water to cook are 3 times, and the time decocted every time is respectively 1.5-2.5h, 1.5-2.5h, 1.0-2.0h.
In above-mentioned preparation method, it is preferred that the concentrate A, concentrate B, concentrate C relative density control
1.1-1.2。
In above-mentioned preparation method, it is preferred that the Herba Dendrobii extract includes that dendrobium extract, stem of noble dendrobium juice or the stem of noble dendrobium are super
Micro mist.
It is furthermore preferred that the preparation of the dendrobium extract includes the following steps：The stem of noble dendrobium is added water to cook 2 times, every time
1.5-2h merges decoction liquor, and filtering, it is 1.1-1.2 that filtrate, which is concentrated into relative density, obtains the dendrobium extract.
It is furthermore preferred that the preparation of the stem of noble dendrobium juice includes the following steps：It is put into beater after the stem of noble dendrobium is softened
Mashing, then be placed in colloid mill and be finely ground to slag uniformity, 80 mesh straining machines are crossed, the stem of noble dendrobium juice is obtained.
It is furthermore preferred that the preparation of the dendrobe ultrafine powder includes the following steps：The stem of noble dendrobium is dry at 50 DEG C -60 DEG C
Processing, it is 2%-3% to make its water content, is placed in 10~30min of crushing in micronizer, obtains the dendrobe ultrafine powder.
The more preferable dendrobe ultrafine powder of above Herba Dendrobii extract because this method be not only simple it is easy, and can increase effectively
The dissolution rate of ingredient, even liposoluble constituent (such as alkaloids, flavonoids active ingredient) improves bioavilability, increases
Strong curative effect of medication.
In above-mentioned preparation method, it is preferred that the relative density for continuing to be concentrated into extracting solution in the step is 1.3~
1.4；The drying is vacuum drying, and temperature is 50~70 DEG C.
Compared with the prior art, the advantages of the present invention are as follows：
1. the present invention carries out compatibility using deer horn, tortoiseshell, Radix Codonopsis, the fruit of Chinese wolfberry, the stem of noble dendrobium, these ingredients contain relatively rich
The effects that carbohydrate, glycosides compound, such components all have immune, anti-oxidant enhancing, anti-aging, different level can be activated
Immune system plays drug effect.
2. the present invention, which is that tortoiseshell, deer horn, the stem of noble dendrobium, Radix Codonopsis, the fruit of Chinese wolfberry are jointly obtained, has enhancing body's immunity
Chinese medicine composition.We are by Sero-pharmacological experiment method to 5 taste simples in the Chinese medicine composition and formula of the present invention
The immunoregulation effect of material extract compares research, and research finds the immunological enhancement of the Chinese medicine composition of the present invention
Preferably, it is better than single medicinal material extract, this generates new compound after may having synergistic effect or compatibility with 5 taste drug matchings
It is related to play immunological enhancement.
3. there is nourishing spleen and stomach, tonifying both YIN and YANG, qi and blood to take into account, consolidate and help member, eliminating evil conditioning for the Chinese medicine composition of the present invention
Effect, and the function of strengthen immunity is apparent, safe.
4. the present invention based on traditional Chinese medical theory, reasonably optimizes preparation process using modernization extraction and separation technology,
Important prescription with immune enhancing function is prepared into novel a variety of compound preparations, improves pharmaceutical activity；And the present invention
Preparation method it is easy to operate, environmentally friendly, economical, efficient, nontoxic, have broad application prospects.
Specific implementation mode
To facilitate the understanding of the present invention, present invention work more comprehensively, is meticulously described below in conjunction with preferred embodiment,
But the protection scope of the present invention is not limited to the following specific embodiments.
Unless otherwise defined, all technical terms used hereinafter and the normally understood meaning of those skilled in the art
It is identical.Technical term used herein is intended merely to the purpose of description specific embodiment, is not intended to the limitation present invention
Protection domain.
Unless otherwise specified, various raw material, reagent, the instrument and equipment etc. used in the present invention can pass through city
Field is commercially available or can be prepared by existing method.
Embodiment 1
A kind of preparation method of the Chinese medicine composition of the enhancing immune function of the present invention, includes the following steps：By CORNU CERVIELAPHI,
Tortoise plastron, Radix Codonopsis, the fruit of Chinese wolfberry and HERBA DENDROBII clean, cutting and drying, and taking 500g deer horns, material is put into extraction tank only, extracting in water
3 times, extraction time is respectively：30h, 30h, 20h merge No. 3 extracting solutions, and filtering, it is 1.1 that filtrate, which is concentrated into relative density, is obtained
Concentrate B；Taking 600g tortoise plastrons, material is put into extraction tank only, and extracting in water 3 times merges extracting solution three times every time for 24 hours, filters, filter
It is 1.1 that liquid, which is concentrated into relative density, obtains concentrate A；114g Radix Codonopsis, the net medicinal material of the 226g fruits of Chinese wolfberry are taken, is added water to cook 3 times, is decocted
Time is respectively 2h, 2h, 1.5h, merges extracting solution three times, filtering, it is 1.1 that filtrate, which is concentrated into relative density, obtains concentrate C；It takes
The 480g stems of noble dendrobium add water to cook twice, each 1.5h, collecting decoction, and filtering, it is 1.1 that filtrate, which is concentrated into relative density, obtains the stem of noble dendrobium and carries
Take object concentrate；Merge above-mentioned each concentrate, mixing continues to be concentrated into relative density to be 1.3 thick pastes, dry, pulverize at 50 DEG C
At fine powder, the as dry medicinal powder of extraction of the invention；Or above-mentioned concentrate is taken to merge, for 24 hours, filtering, filtrate is the present invention to alcohol precipitation
Extraction liquid.
Embodiment 2：
A kind of preparation method of the Chinese medicine composition of the enhancing immune function of the present invention, includes the following steps：By sika deer
Angle, tortoise plastron, Radix Codonopsis, the fruit of Chinese wolfberry and Dendrobidium huoshanness clean, cutting and drying, and taking 600g CORNU CERVIs, material is put into extraction tank only,
Extracting in water 3 times, extraction time be respectively：26h, 26h, 16h, merge No. 3 extracting solutions, filtering, and filtrate is concentrated into relative density
It is 1.2, obtains concentrate B；Taking 700g tortoise plastrons, material is put into extraction tank only, and extracting in water 3 times, each 16h, merging is extracted three times
Liquid, filtering, it is 1.2 that filtrate, which is concentrated into relative density, obtains concentrate A；160g Radix Codonopsis, the net medicinal material of the 210g fruits of Chinese wolfberry are taken, decocting is added
It boils 3 times, decocting time is respectively 1.5h, 1.5h, 1.0h, merges extracting solution three times, filtering, and filtrate is concentrated into relative density and is
1.2, obtain concentrate C；The 420g stems of noble dendrobium are taken to add water to cook twice, each 1.5h, collecting decoction, filtering, filtrate is concentrated into relatively close
Degree is 1.2, obtains Herba Dendrobii extract concentrate；Merging above-mentioned each concentrate, mixing continues to be concentrated into relative density to be 1.3 thick pastes,
Fine powder, the as dry medicinal powder of extraction of the invention are dried, crushed at 70 DEG C；Or above-mentioned concentrate is taken to merge, alcohol precipitation for 24 hours, filters,
Filtrate is the extraction liquid of the present invention.
Embodiment 3：
A kind of preparation method of the Chinese medicine composition of the enhancing immune function of the present invention, includes the following steps：By pilose antler, tortoise
First, Radix Codonopsis, the fruit of Chinese wolfberry and stem of Eyeshaped Dendrobium clean, cutting and drying, and taking 650g pilose antlers, material is put into extraction tank only, extracting in water 3
Secondary, extraction time is respectively：34h, 34h, for 24 hours merges No. 3 extracting solutions, and filtering, it is 1.2 that filtrate, which is concentrated into relative density, is obtained dense
Contracting liquid B；Taking 500g tortoise plastrons, material is put into extraction tank only, and extracting in water 3 times, each 20h merges extracting solution three times, filtering, filtrate
It is 1.2 to be concentrated into relative density, obtains concentrate A；100g Radix Codonopsis, the net medicinal material of the 300g fruits of Chinese wolfberry are taken, is added water to cook 3 times, when decoction
Between be respectively 2.5h, 2.5h, 2.0h, merge extracting solution three times, filtering, it is 1.2 that filtrate, which is concentrated into relative density, obtains concentrate C；
The 400g stems of noble dendrobium are taken to add water to cook twice, each 2.0h, collecting decoction, filtering, it is 1.2 that filtrate, which is concentrated into relative density, obtains the stem of noble dendrobium
Extract concentrated solution；Merge above-mentioned each concentrate, mixing continues to be concentrated into relative density to be 1.3 thick pastes, be dried at 60 DEG C, powder
It is broken into fine powder, the as dry medicinal powder of extraction of the invention；Or above-mentioned concentrate is taken to merge, for 24 hours, filtering, filtrate is this hair to alcohol precipitation
Bright extraction liquid.
Embodiment 4：
A kind of preparation method of the Chinese medicine composition of the enhancing immune function of the present invention, includes the following steps：By deer horn support
Disk, plastron, Radix Codonopsis, the fruit of Chinese wolfberry and dendrobium candidum clean, cutting and drying, and taking 700g deer horns pallet, material is put into extraction tank only,
Extracting in water 3 times, extraction time be respectively：30h, 30h, 20h, merge No. 3 extracting solutions, filtering, and filtrate is concentrated into relative density
It is 1.1, obtains concentrate B；Taking 680g plastrons, material is put into extraction tank only, and extracting in water 3 times, every time for 24 hours, merging extract three times
Liquid, filtering, it is 1.1 that filtrate, which is concentrated into relative density, obtains concentrate A；126g Radix Codonopsis, the net medicinal material of the 226g fruits of Chinese wolfberry are taken, decocting is added
It boils 3 times, decocting time is respectively 2h, 2h, 1.5h, merges extracting solution three times, filtering, it is 1.1 that filtrate, which is concentrated into relative density, is obtained
Concentrate C；The 460g stems of noble dendrobium are taken to add water to cook twice, each 1.5h, collecting decoction filters, and filtrate is concentrated into relative density and is
1.1, obtain Herba Dendrobii extract concentrate；Merge above-mentioned each concentrate, mixing, it is 1.3 thick pastes to continue to be concentrated into relative density, 70 DEG C
Under be dried, crushed into fine powder, the as dry medicinal powder of extraction of the invention；Or above-mentioned concentrate is taken to merge, alcohol precipitation for 24 hours, filters, filtrate
As extraction liquid of the invention.
Embodiment 5：
A kind of preparation method of the Chinese medicine composition of the enhancing immune function of the present invention, includes the following steps：By cornu cerve degelatinatum,
Tortoise plastron, Radix Codonopsis, the fruit of Chinese wolfberry and Dendrobium denneanum clean, cutting and drying, and taking 600g cornu cerve degelatinatums, material is put into extraction tank only, and water is added to carry
It takes 3 times, the time decocted every time is respectively：30h, 30h, 20h, merge No. 3 extracting solutions, filtering, and filtrate is concentrated into relative density
It is 1.1, obtains concentrate B；Taking 600g tortoise plastrons, material is put into extraction tank only, and extracting in water 3 times, every time for 24 hours, merging extract three times
Liquid, filtering, it is 1.1 that filtrate, which is concentrated into relative density, obtains concentrate A；114g Radix Codonopsis, the net medicinal material of the 226g fruits of Chinese wolfberry are taken, decocting is added
It boils 3 times, decocting time is respectively 2h, 2h, 1.5h, merges extracting solution three times, filtering, it is 1.1 that filtrate, which is concentrated into relative density, is obtained
Concentrate C；Taking the 400g stems of noble dendrobium to be dried at 60 DEG C makes water content be 2%, sets and crushes 30min in micronizer, obtains the stem of noble dendrobium
Ultramicro-powder；Merge above-mentioned each concentrate, mixing continues to be concentrated into relative density to be 1.3 thick pastes, is added dendrobe ultrafine powder, 70 DEG C
Under be dried, crushed into fine powder, the as dry medicinal powder of extraction of the invention；Or above-mentioned concentrate is taken to merge, alcohol precipitation for 24 hours, filters, filtrate
As extraction liquid of the invention.
Embodiment 6：
A kind of preparation method of the Chinese medicine composition of the enhancing immune function of the present invention, includes the following steps：By CORNU CERVIELAPHI,
Tortoiseshell, Radix Codonopsis, the fruit of Chinese wolfberry and Dendrobium chrysanthum clean, cutting and drying, and taking 600g deer horns, material is put into extraction tank only, extracting in water
3 times, extraction time is respectively：30h, 30h, 20h merge No. 3 extracting solutions, and filtering, it is 1.2 that filtrate, which is concentrated into relative density, is obtained
Concentrate B；Taking 600g tortoiseshells, material is put into extraction tank only, and extracting in water 3 times merges extracting solution three times every time for 24 hours, filters, filter
It is 1.2 that liquid, which is concentrated into relative density, obtains concentrate A；114g Radix Codonopsis, the net medicinal material of the 226g fruits of Chinese wolfberry are taken, is added water to cook 3 times, is decocted
Time is respectively 2h, 2h, 1.5h, merges extracting solution three times, filtering, it is 1.2 that filtrate, which is concentrated into relative density, obtains concentrate C；It takes
400g dendrobium fresh strips are put into beater after softening and are beaten, then set and be finely ground to slag uniformity in colloid mill, cross 80 mesh sievings
Machine, filtrate merge with above-mentioned each concentrate, mixing, continue to be concentrated into relative density to be 1.3 thick pastes, are dried, crushed at 60 DEG C
Fine powder, the as dry medicinal powder of extraction of the invention；Or above-mentioned concentrate is taken to merge, for 24 hours, filtering, filtrate is the present invention's to alcohol precipitation
Extract liquid.
Embodiment 7：
140g starch will be added in the dry medicinal powder of any extraction in the various embodiments described above, mixing is pelletized, and sieving fills 1000
Grain capsule, the as capsule of the Chinese medicine composition of enhancing immune function of the invention.
Embodiment 8：
Lactose 100g, dextrin 500g, granulation, whole grain, mistake will be added in the dry medicinal powder of any extraction in the various embodiments described above
1000g particles, the as granule of the Chinese medicine composition of enhancing immune function of the invention is made in sieve.
Embodiment 9：
Starch 510g, dextrin 102g, superfine silica gel powder 34g will be added in the dry medicinal powder of any extraction in the various embodiments described above,
It using 90% ethyl alcohol as wetting agent softwood, pelletizes, dry, whole grain adds sodium carboxymethylcellulose 50g, magnesium stearate 4g, always
Mixed, the tablet of 0.3g/ piece specifications, the as tablet of the Chinese medicine composition of enhancing immune function of the invention is made in tabletting.
Embodiment 10：
0.3% sodium benzoate being added in the dry liquid of any extraction in the various embodiments described above, 400ml distilled water is filling,
1000ml oral solutions, the as oral solution of the Chinese medicine composition of enhancing immune function of the invention is made in sterilizing.
Enhance the experimental study of immune function
1) to the influence of mouse immune organ organ index
Any extraction liquid in above-described embodiment 1~6 is taken, is diluted with water one times, through stomach perfusion to mouse, daily one
Secondary, continuous 21d puts to death animal, immediately dissection and separating spleen, thymus gland, after sucking surface moisture with filter paper, electronic analytical balance
Weight in wet base is weighed, Immune Organs Index is calculated according to the following formula：Spleen weight index=spleen quality (mg)/weight × 100%, chest gland weight
Index=thymic factor D injection (mg)/weight × 100%, compared with the control group its otherness.Test data is soft using SPSS 12.0
Part is analyzed, and the results are shown in Table 1：
Influence of the table 1 to mouse immune organ organ index
Note：Compared with blank control, significant difference (P<0.05)
As shown in Table 1, after gavage is to extraction the liquid 0.2ml/10g.d, 21d of the present invention, compared with physiological saline group, this
The extraction liquid of invention has apparent rush to the immune organ spleen of mouse, the growth and development of thymus gland and the increment of immunocompetent cell
Into effect.
2) to the influence of mouse monokaryon macrophage phagocytic function
Any extraction liquid in above-described embodiment 1~6 is taken, is diluted with water one times, through stomach perfusion to mouse, daily one
It is secondary, continuous 21d, 1h, 25% india ink 20ml/kg of mouse tail vein injection after being administered every time, 1min, 5min points after injection
Do not take a blood sample from the orbital venous plexus of mouse 20 μ l, is dissolved in the 0.1%NaCO of 3ml3It is shaken up in solution, in purple after standing 20min
OD values are measured on outer spectrophotometer under 680nm, with 0.1%NaCO3Solution is blank control；It is taken after mouse cervical dislocation is put to death
Mouse Liver, spleen weigh after blotting blood with filter paper, calculated as described below the formula unit of account time clean up index and phagocytic index
(α)：Clean up index=(lgOD1- lgOD5)/(t5- t1), phagocytic index (α)=K × weight/(liver weight+spleen weight) counts medicine
K, α value of object group and control group, and carry out t value tests.Test data is analyzed using 12.0 softwares of SPSS, as a result such as table 2
It is shown：
Influence of the table 2 to mouse monokaryon macrophage phagocytic function
Note：Compared with blank control, significant difference (P<0.05)
As shown in Table 2, after gavage is to the present invention extraction liquid 0.2ml/10g.d, 21d, mouse cleans up index and phagocytosis
Index (α) is relative to control group, otherness significantly (P<0.05), i.e., it is huge significantly to improve monokaryon-to extraction liquid of the invention
The phagocytic activity of phagocyte, to enhance immunity of organisms.
Compare the immunoregulation effect of Traditional Chinese medicine compound composition and 5 taste single medicinal substances extracts in side
(1) preparation of extract
The preparation of Cornu Cervi extract：Taking 500g deer horns, material is put into extraction tank only, extracting in water 3 times, extraction time difference
For：30h, 30h, 20h, merge No. 3 extracting solutions, filtering, and filtrate is concentrated into relative density as 1.1 to get Cornu Cervi extract.
The preparation of tortoiseshell extract：Taking 500g tortoiseshells, material is put into extraction tank only, and extracting in water 3 times merges every time for 24 hours
Extracting solution three times, filtering, filtrate are concentrated into relative density as 1.1 to get tortoiseshell extract.
The preparation of Radix Codonopsis extract：Take the net medicinal material of 114g Radix Codonopsis, add water to cook 3 times, decocting time be respectively 2h, 2h,
1.5h, merges extracting solution three times, filtering, and filtrate is concentrated into relative density as 1.1 to get Radix Codonopsis extract.
The preparation of wolfberry fruit extract：Take the net medicinal material of the 226g fruits of Chinese wolfberry, add water to cook 3 times, decocting time be respectively 2h, 2h,
1.5h, merges extracting solution three times, filtering, and filtrate is concentrated into relative density as 1.1 to get wolfberry fruit extract.
The preparation of Herba Dendrobii extract：The 500g stems of noble dendrobium are taken to add water to cook twice, each 90min, collecting decoction, filtering, filtrate
Relative density is concentrated into as 1.1 to get Herba Dendrobii extract.
The preparation of Traditional Chinese medicine compound composition：By said extracted method, merge the extracting solution of 5 taste medicines, mixing is concentrated into opposite
Density is 1.1 to get Traditional Chinese medicine compound composition.
(2) preparation of Contained Serum
Mouse is randomly divided into 6 groups, and every group 10, experimental group gavages Traditional Chinese medicine compound composition, Carapax Et Plastrum Testudinis extract, deer horn respectively
Extract, Radix Codonopsis extract, wolfberry fruit extract, Herba Dendrobii extract, control group gavage physiological saline, fill 1 (0.5ml/ daily
Only/time), continuous 7 days, mouse extractd eyeball after last fills 1h and takes blood, sterile separation serum, through 56 after same group serum mixing
DEG C, 30min inactivation treatments, then with 0.45um filtering with microporous membrane degermings, set -20 DEG C and save backup.
(3) experiment grouping
Contained Serum group is divided into 7 groups, i.e. compound serum group, Carapax Et Plastrum Testudinis extract serum group, Cornu Cervi extract serum
Group, Radix Codonopsis extract's serum group, wolfberry fruit extract serum group, Herba Dendrobii extract serum group, blank serum control group.
(4) lymphocyte proliferation assay
Cervical dislocation puts to death mouse, 2~3min of immersion in 75% alcohol is set, in the superclean bench crossed through ultra violet lamp
Upper sterile taking-up mouse spleen.Spleen surface fat and connective tissue are rejected, surface blood is cleaned with antibiotic Hanks liquid
Mark.Spleen is cut into small pieces, continues to be rinsed to limpid with antibiotic Hanks liquid.Spleen fritter is placed in 200 mesh filter screens
On, it is gently twisted into pieces with sterilizing syringe needle core, while being rinsed with RPMI-1640 liquid, collects 1640 containing splenocyte and rinse
Liquid, 1500r/min centrifuge 5min.It goes after supernatant that the 0.83%Tris-NH of precooling is added4Cl splitting erythrocyte 1min, repetition are split
Solution is primary, then is washed 2~3 times with 1640 liquid, abandons supernatant, mixing.Through 0.1% Trypan Blue, check that dead cell number is less than 5%,
It is with the RPMI-1640 complete culture solutions (penicillin containing 100U/ml, 100U/ml streptomysins) containing 10% calf serum that spleen is thin again
Born of the same parents are diluted to 6 × 106The cell suspension of a/ml.100ul cell suspensions, test group is added per hole in 96 hole flat-bottomed plates
Drug or Contained Serum 100ul to be measured, control group is added to add culture solution or gavage the serum 100ul prepared after physiological saline, per hole
Do 3 repeating holes.Culture plate is put into 37 DEG C, 5%CO2Incubator in cultivate 72h, in terminate cultivate before 4h, per hole be added
10mg/ml MTT solution 10ul continue to cultivate 4h, carefully be discarded supernatant after culture, per the HCl dropwise isopropanol 100ul in hole,
20min fully is stood after oscillation piping and druming, with Detection wavelength 570nm reference wavelength 630nm colorimetric analysis, result is indicated with OD values.
(5) test results and analysis
It is handled using SPSS16.0 statistics softwares, group difference compares using single factor test variance (one way ANOVA)
Analysis.The results are shown in Table 3：
Table 3：The influence that 7 Contained Serum groups are proliferated mouse lymphocyte
Note:Number right side * indicates comparison among groups significant difference (P in table<0.05), * * indicate that comparison among groups difference is extremely different notable
(P<0.01)。
Above-mentioned experimental method is to give after animal gavages Chinese medicine or compound preparation, takes a blood sample within a certain period of time, detaches serum
The method for carrying out external pharmacological evaluation, which overcome the unfavorable factors of previous TCM in Vitro experiment, can be more objective and truly explain
The drug effect and mechanism of action of bright Chinese medicine, to contribute to Study of Traditional Chinese Medicine compound compatibility rule and promote Chinese medicine compound prescription pharmacokinetics
Research is carried out in a deep going way.
It is shown by the result of table 3：In 7 drug serum groups, Codonopsis pilosula and matrimony vine extract drug serum is to mouse lymphocyte
The facilitation of proliferation is weaker, does not have difference, other 5 drug serum groups to have the proliferation of mouse lymphocyte with control group
There is different degrees of humidification, generates pole significant difference with control group；7 drug serum groups are to mouse lymphocyte
The strong and weak sequence of the facilitation of proliferation is followed successively by：Traditional Chinese medicine compound composition serum group>Carapax Et Plastrum Testudinis extract serum group>Cornu Cervi extract
Serum group>Radix Codonopsis extract's serum group>Herba Dendrobii extract serum group>Wolfberry fruit extract serum group>Blank serum control group；With 5
The drug serum group of a medicinal substances extract containing single compares, and the drug serum group containing Traditional Chinese medicine compound composition can remarkably promote mouse
Lymphopoiesis is significantly stronger than other medicines serum group, and all has significant difference (P<0.01).
(6) conclusion
This result of study shows that the drug serum of Traditional Chinese medicine compound composition of the present invention is anti-to mouse lymphocyte in-vitro multiplication
There should be significant humidification, and its effect is better than the drug serum of single medicinal substances extract respectively, this may be with 5 taste Chinese medicines
Compatibility is related with new compound performance immunological enhancement is generated after synergistic effect or compatibility.
Claims (9)
1. a kind of Traditional Chinese medicine compound composition of improving immunocompetence, which is characterized in that the Traditional Chinese medicine compound composition is by multi-flavor
The respective extracting solution of Chinese medicine is prepared after mixing, concentrating；
Aforementioned kinds of traditional Chinese medicines is each component of following mass fraction proportioning：
Tortoiseshell 25%~36%
Deer horn 25%~36%
The stem of noble dendrobium 19%~25%
5%~8% He of Radix Codonopsis
The fruit of Chinese wolfberry 10%~17%.
2. Traditional Chinese medicine compound composition according to claim 1, which is characterized in that the tortoiseshell is tortoise plastron, plastron or tortoise plastron
Glue；The deer horn is CORNU CERVIELAPHI, CORNU CERVI, pilose antler, cornu cerve degelatinatum, deer horn glue or deer horn pallet；The stem of noble dendrobium be HERBA DENDROBII,
Dendrobidium huoshanness, dendrobium candidum, stem of Eyeshaped Dendrobium, Dendrobium Chrysotoxum Lindl, Dendrobium denneanum, Dendrobium chrysanthum, Dendrobium fimbriatum Hook or Dendrobium loddigesii.
3. Traditional Chinese medicine compound composition according to claim 1, which is characterized in that the Chinese medicine composition is oral granule
Agent, tablet, capsule or oral solution.
4. a kind of preparation method of Traditional Chinese medicine compound composition according to any one of claims 1 to 3, includes the following steps：
Ready raw material tortoiseshell, deer horn, the stem of noble dendrobium, Radix Codonopsis, the fruit of Chinese wolfberry are cleaned, cutting and drying, by corresponding mass fraction
Proportioning is weighed；
The tortoiseshell, deer horn are put into extraction tank respectively, added water to cook, after respective merging decoction liquor is filtered, respectively
To concentrate A and concentrate B；
The Radix Codonopsis, fruit of Chinese wolfberry merging are put into extraction tank, is added water to cook, is filtered after merging decoction liquor, obtain concentrate C；
Raw material stem of noble dendrobium processing is prepared into Herba Dendrobii extract；
Concentrate A obtained above, concentrate B, concentrate C are merged with Herba Dendrobii extract, then proceed to concentration, dry, powder
It is broken, and it is suitably added auxiliary material as needed, obtain the Traditional Chinese medicine compound composition.
5. preparation method according to claim 4, which is characterized in that the number added water to cook is 2-4 times；Work as decoction
When tortoiseshell or deer horn, the time decocted every time is 16-34 h；When decocting Radix Codonopsis, the fruit of Chinese wolfberry, the time decocted every time is 1.0-
2.5 h。
6. preparation method according to claim 5, which is characterized in that when decocting deer horn, the number added water to cook is 3 times,
The time decocted every time is respectively 26-34 h, 26-34 h, 16-24 h；When decocting tortoiseshell, the number added water to cook is 3 times, often
The time of secondary decoction is 16-24 h；When decocting Radix Codonopsis and the fruit of Chinese wolfberry, the number added water to cook is 3 times, the time decocted every time
Respectively 1.5-2.5 h, 1.5-2.5 h, 1.0-2.0 h.
7. preparation method according to claim 4, which is characterized in that the concentrate A, concentrate B, concentrate C phase
Density is controlled in 1.1-1.2.
8. preparation method according to claim 4, which is characterized in that the Herba Dendrobii extract includes dendrobium extract, stone
Dry measure used in former times juice or dendrobe ultrafine powder；
The preparation of the dendrobium extract includes the following steps：The raw material stem of noble dendrobium is added water to cook 2 times, each 1.5-2.0 h,
Merge decoction liquor, filtering, it is 1.1-1.2 that filtrate, which is concentrated into relative density, obtains the dendrobium extract；
The preparation of the stem of noble dendrobium juice includes the following steps：It is put into beater and is beaten after the raw material stem of noble dendrobium is softened, then set
It is finely ground to slag uniformity in colloid mill, crosses straining machine, obtains the stem of noble dendrobium juice；
The preparation of the dendrobe ultrafine powder includes the following steps：The raw material stem of noble dendrobium is dried at 50 DEG C -60 DEG C, makes it
Water content is 2%-3%, then is placed in crushing 10-30 min in micronizer, obtains the dendrobe ultrafine powder.
9. preparation method according to claim 4, which is characterized in that it is described continue concentration when until extracting solution it is relatively close
Degree reaches 1.3-1.4；Temperature when described dry is 50 DEG C -70 DEG C.
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