CN104977779B - A kind of device and method of auto-focusing - Google Patents
A kind of device and method of auto-focusing Download PDFInfo
- Publication number
- CN104977779B CN104977779B CN201510439170.4A CN201510439170A CN104977779B CN 104977779 B CN104977779 B CN 104977779B CN 201510439170 A CN201510439170 A CN 201510439170A CN 104977779 B CN104977779 B CN 104977779B
- Authority
- CN
- China
- Prior art keywords
- light beam
- detection light
- object lens
- solid
- liquid interface
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention discloses a kind of device and method of auto-focusing, the device passes through laser, fiber coupler, inclined surface fiber, collimating element, object lens, the first semi-transparent semi-reflecting lens, the first relay lens, CCD/CMOS imaging devices and controller, to adjust the position of object lens, so that the focus of object lens can be adjusted quickly to the optimum position of cell imaging to be measured.The embodiment of the present invention can quickly and accurately realize auto-focusing, solve to shoot multi-pass operation, slow-footed problem of focusing during picture.
Description
Technical field
The present invention relates to technical field of optical detection, more particularly to a kind of device and method of auto-focusing.
Background technology
In the research field of cell, the real-time monitored of cell is very important link, including the shape of observation cell
The growth of cell and the activity situation of cell are studied in state, division etc..
In the lab, typically it is grown on the bottom surface of culture box for the cell studied, and cell is constantly being moved,
There is refringence the bottom surface of wherein cell and culture box, and also there is refringence in outside air and culture cassette bottom face, and the two is easy
Reflection is caused, so when observation cell by cell is shot, it is accurate and quick that capture apparatus carries out auto-focusing needs.
At present, the method for capture apparatus auto-focusing is mainly two kinds, and one is to be continuously shot plurality of pictures, at image
Reason, the acutance of the every pictures of contrast, brightness and contrast, chooses the best pictures of shooting effect；Be for second by away from
From sensor, the distance between object and camera lens are measured, optimum position is found in multiple ranging.But both approaches be required for it is many
Secondary operation, focusing speed is slower.
The content of the invention
It is an object of the invention to provide a kind of device and method of auto-focusing, can quickly and accurately it realize automatic
Focusing, solves to shoot multi-pass operation, slow-footed problem of focusing during picture.
For up to this purpose, the present invention uses following technical scheme：
In a first aspect, the invention provides a kind of device of auto-focusing, including：
Laser, for emission detection light beam；
Fiber coupler, for carrying out coupling processing to the detection light beam launched from laser；
Inclined surface fiber, for transmitting detection light beam and detection light beam being divided into along the asymmetric first detection light of optical axis
Beam and the second detection light beam；
Collimating element, for the first detection light beam and the second detection light beam to be carried out into collimation processing；
Object lens, for receiving detection light beam, make the first detection light beam and the second detection light beam be focused at cell culture to be measured
On solid-liquid interface in box；
First semi-transparent semi-reflecting lens, the beam splitter for being reflected to solid-liquid interface is the first Returning beam and second
Returning beam；
First relay lens, for being corrected and focusing to the first Returning beam；
CCD/CMOS imaging devices, for receiving the first Returning beam to form hot spot, and light spot image information are sent
To controller；
Controller, for according to the distance between optimum position and solid-liquid interface of cell imaging to be measured in culture box with
And the light spot image information that CCD/CMOS imaging devices are sent, the position of object lens is adjusted, so that the focus of object lens is in cell to be measured
On the optimum position of imaging.
Second aspect, the embodiment of the present invention additionally provides a kind of method of auto-focusing, including：
The detection light beam launched from laser is carried out by laser emission detection light beam, and by fiber coupler
Coupling processing；
It is divided into by inclined surface fiber transmission detection light beam and by detection light beam along the asymmetric first detection light of optical axis
Beam and the second detection light beam；
First detection light beam and the second detection light beam are carried out by collimation processing by collimating element；
Detection light beam is received by object lens, the first detection light beam and the second detection light beam is focused at cell culture case to be measured
In solid-liquid interface on；
The beam splitter reflected by the first semi-transparent semi-reflecting lens to solid-liquid interface is the first Returning beam and second
Returning beam；
The first Returning beam is corrected by the first relay lens and focusing；
First Returning beam is received to form hot spot by CCD/CMOS imaging devices, and light spot image information is sent to
Controller；
By controller according to the distance between optimum position and solid-liquid interface of cell imaging to be measured in culture box with
And the light spot image information that CCD/CMOS imaging devices are sent, the position of object lens is adjusted, so that the focus of object lens is in cell to be measured
On the optimum position of imaging.
Beneficial effects of the present invention are：A kind of device and method of auto-focusing of the present invention, will be visited by sloping optical fiber
Light-metering beam is divided into along the asymmetric first detection light beam of optical axis and the second detection light beam；By collimating element to the first detection light beam
Collimation processing is carried out with the second detection light beam；The first detection light beam and the second detection light beam are focused at by object lens to be measured thin
On solid-liquid interface in born of the same parents' culture box；Beam splitter solid-liquid interface reflected by the first semi-transparent semi-reflecting lens is the
One Returning beam and the second Returning beam；First Returning beam is received by CCD/CMOS imaging devices to form hot spot, and will
Light spot image information is sent to controller, and controller is according to the optimum position of cell imaging to be measured and solid-liquid interface in culture box
The distance between and CCD/CMOS imaging devices send light spot image information, adjust object lens position so that Jiao of object lens
Point is on the optimum position of cell imaging to be measured.The device of the auto-focusing of the present invention can quickly and accurately be realized automatic right
Jiao, solves to shoot multi-pass operation, slow-footed problem of focusing during picture.
Brief description of the drawings
By reading the detailed description made to non-limiting example made with reference to the following drawings, of the invention is other
Feature, objects and advantages will become more apparent upon：
Fig. 1 is a kind of apparatus structure block diagram for auto-focusing that the embodiment of the present invention one is provided；
Fig. 2 is a kind of apparatus structure block diagram for auto-focusing that the embodiment of the present invention two is provided；
Fig. 3 is the PZ axial curve figures formed on the first photodetector that the embodiment of the present invention two is provided；
Fig. 4 is a kind of apparatus structure block diagram for auto-focusing that the embodiment of the present invention three is provided；
Fig. 5 is the apparatus structure block diagram for another auto-focusing that the embodiment of the present invention three is provided；
Fig. 6 is the method flow diagram for the first auto-focusing that the embodiment of the present invention four is provided；
Fig. 7 is the method flow diagram for second of auto-focusing that the embodiment of the present invention four is provided；
Fig. 8 is the method flow diagram for the third auto-focusing that the embodiment of the present invention four is provided；
Fig. 9 is the method flow diagram for the 4th kind of auto-focusing that the embodiment of the present invention four is provided.
Embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples.It is understood that this place is retouched
The specific embodiment stated is used only for explaining the present invention, rather than limitation of the invention.It also should be noted that, in order to just
Part related to the present invention rather than full content are illustrate only in description, accompanying drawing.
Embodiment one
Fig. 1 is a kind of apparatus structure block diagram for auto-focusing that the embodiment of the present invention one is provided, as shown in figure 1, the dress
Put including：Laser 101, fiber coupler 102, inclined surface fiber 103, collimating element 104, object lens 105, first are semi-transparent semi-reflecting
Mirror 106, the first relay lens 107, CCD/CMOS imaging devices 108 and controller 109.
Wherein, laser 101, for emission detection light beam；
Fiber coupler 102, for carrying out coupling processing to the detection light beam launched from laser 101.
Inclined surface fiber 103, is visited for transmitting detection light beam and detection light beam being divided into along optical axis asymmetric first
Light-metering beam and the second detection light beam；Wherein, inclined surface fiber 103 is single-mode fiber, using FC/APC fiber connectors, makes single mode
The exiting surface of optical fiber is inclined-plane.The the first detection light beam come out by the exiting surface of inclined surface fiber 103 and the second detection light beam edge
Optical axis is asymmetric (quantity of the light of the first detection light beam and the second detection light beam is different).
Collimating element 104, for the first detection light beam and the second detection light beam to be carried out into collimation processing；Wherein, member is collimated
Part is preferably collimating mirror or beam expanding lens.
Object lens 105, for receiving detection light beam, make the first detection light beam and the second detection light beam be focused at cell to be measured
On solid-liquid interface in 100 culture boxes；
First semi-transparent semi-reflecting lens 106, for the beam splitter that is reflected to solid-liquid interface for the first Returning beam and
Second Returning beam.Wherein, first semi-transparent semi-reflecting lens 106 can also be preferably optical filter, polarization light splitting piece or spectroscope
Deng；The the first detection light beam and half of the half reflected are respectively contained in first Returning beam and the second Returning beam
Second detection light beam.
First relay lens 107, for being corrected and focusing to the first Returning beam；
CCD/CMOS imaging devices 108, for receiving the first Returning beam to form hot spot, and light spot image information are sent out
Give controller 109；
Controller 109, for optimum position and the solid-liquid interface according to cell imaging to be measured in default culture box it
Between distance and CCD/CMOS imaging devices 108 send light spot image information, adjust object lens 105 position so that object lens
105 focus is on the optimum position that cell 100 to be measured is imaged.Wherein, solid-liquid interface can reflect the first spy in culture box
Light-metering beam and the second detection light beam, have a certain distance H between the optimum position of the imaging of cell 100 to be measured and solid-liquid interface,
, it is necessary to which H is set before being tested.
On the basis of above-described embodiment, first semi-transparent semi-reflecting lens 106 are additionally operable to the first detection light beam and second
Detection light beam is reflected, so that the first detection light beam and the second detection light beam enter object lens 105.
The said apparatus course of work is as follows：After the emission detection light beam of laser 101, coupled by fiber coupler 102,
It is divided into by the transmission of inclined surface fiber 103 detection light beam and by detection light beam along the asymmetric first detection light beam and second of optical axis
Detect light beam；First detection light beam and the second detection light beam are carried out collimation processing by collimated element 104, semi-transparent semi-reflecting through first
Mirror 106 is reflected into object lens 105.
Into after object lens 105, the first detection light beam and the second detection light beam is set to be focused on the solid-liquid interface of culture box,
Solid-liquid interface is reflected the first detection light beam and the second detection light beam respectively, and the light beam being reflected back is semi-transparent semi-reflecting through first
The beam splitting of mirror 106 turns into the first Returning beam and the second Returning beam, and the first Returning beam is connect by CCD/CMOS imaging devices 108
Receive, form hot spot, and light spot image information is sent to controller 109.
Finally, controller 109 adjusts the position of object lens 105, makes Jiao of object lens 105 according to the light spot image information of reception
Point is on solid-liquid interface, and the distance between optimum position according to default cell imaging to be measured and solid-liquid interface H, adjusts
Whole object lens 105, make the focus of object lens 105 in the optimum position of cell imaging to be measured.Wherein, when the focus of object lens is not in solid-liquid point
When on interface, the hot spot of circular brightness irregularities is formed on CCD/CMOS imaging devices, first due to forming hot spot returns
Comprising the first detection light beam being reflected back and the second detection light beam in light beam, the exiting surface of inclined surface fiber is inclined-plane, causes the
One detection light beam and the second detection light beam are asymmetric along optical axis, cause the uneven of hot spot brightness.When the focus of object lens is solid
When on liquid interface, in the hot spot brightness uniformity of CCD/CMOS imaging devices formation.
On the basis of above-described embodiment, above-mentioned hot spot can also be formed by other forms.For example, by angled end-face light
Fibre is substituted for exiting surface and is the optical fiber of flat mouth, but the light beam after collimating element is collimated is blocked into a part, makes after collimation
Light beam it is asymmetric.
In the present embodiment, as a result of inclined surface fiber, make the device internal structure of described auto-focusing simple,
It is easy to operate, and the cost of manufacture of described device can be reduced.
The embodiments of the invention provide a kind of device of auto-focusing, the device passes through laser, fiber coupler, splay end
Face optical fiber, collimating element, object lens, the first semi-transparent semi-reflecting lens, the first relay lens, CCD/CMOS imaging devices and controller, to adjust
The position of whole object lens, so that the focus of object lens is on the optimum position of cell imaging to be measured.The embodiment of the present invention can quickly, essence
Auto-focusing really is realized, solves to shoot multi-pass operation, slow-footed problem of focusing during picture.
Embodiment two
The present embodiment is similar to the structure of embodiment one, differs only in the device of the auto-focusing of the present embodiment and also includes
First photodetector, Fig. 2 is a kind of apparatus structure block diagram for auto-focusing that the embodiment of the present invention two is provided；As shown in Fig. 2
Described device also includes：
First photodetector 110, for receiving the second Returning beam, and converts optical signals into the first electric signal, and
First electric signal is sent to controller 109；
Wherein, in the present embodiment, controller 109, for according to the optimum position that cell 100 to be measured is imaged in culture box
The light spot image information sent with the distance between solid-liquid interface, CCD/CMOS imaging devices 108 and the first photodetection
The first electric signal that device 110 is sent, adjusts the position of object lens 105, so that what the focus of object lens 105 was imaged in cell 100 to be measured
On optimum position.
On the basis of above-described embodiment, as shown in Fig. 2 inclined surface fiber is superfine single-mode fiber, because copolymerization is burnt
Principle, when object lens 105 focus be located at solid-liquid interface or it is very near apart from solid-liquid interface in the case of, the first photoelectricity
Detector 110 can just detect the second Returning beam, and PZ axial curve figures are generated on the first photodetector 110, observe simultaneously
Whether the hot spot brightness formed in CCD/COMS imaging devices 108 is uniform, and controller 109 obtains and records the first photodetector
The position of the peak value of the 110 PZ axial curves detected, further adjustment object lens 105.Fig. 3 is what is formed on the first photodetector
PZ axial curve figures, as shown in figure 3, Z axis represents the position of object lens, the distance between Z1 and Z2 is very small, and Z1 and Z2 represent object lens
Focus closely solid-liquid interface, and Z0 represents that the focus of object lens is located at solid-liquid interface.
After controller adjusts the focus of object lens to solid-liquid interface, according to the optimal of default cell imaging to be measured
Object lens are adjusted, make the focus of object lens in the optimal of cell imaging to be measured by the distance between position and solid-liquid interface H again
On position.
The embodiment of the present invention two provides a kind of device of auto-focusing, and the device is added on the basis of embodiment one
First photodetector, controller can cultivate in box between the optimum position of cell imaging to be measured and solid-liquid interface away from
The first electric signal that the light spot image information sent from, CCD/CMOS imaging devices and the first photodetector are sent, adjustment
The position of object lens.The embodiment of the present invention can quickly and accurately realize auto-focusing, solve repeatedly to grasp during shooting picture
Make, slow-footed problem of focusing.
Embodiment three
The present embodiment three is similar with the structure of embodiment one, differs only in and one group is added on the basis of embodiment one
The device of separately formed hot spot, that is, add the second semi-transparent semi-reflecting lens, the second relay lens and two quadrant photodetector, Fig. 4
It is a kind of device of auto-focusing provided in an embodiment of the present invention, as shown in figure 4, described device, in addition to：
Second semi-transparent semi-reflecting lens 111, for the second Returning beam to be split into the 3rd Returning beam and the 4th return
Light beam；Wherein, the second semi-transparent semi-reflecting lens 111 can also be preferably optical filter, polarization light splitting piece or spectroscope etc..
Second relay lens 112, for being focused and correction process to the 3rd Returning beam；
Two quadrant photodetector 113, for receiving the 3rd Returning beam to form hot spot, and light spot image information is sent out
Give controller 109；Wherein, two chips are contained in two quadrant photodetector.
Wherein, controller 109, for according to the optimum position and solid-liquid interface that cell 100 to be measured is imaged in culture box
The distance between and two quadrant photodetector 113 send light spot image information, adjust object lens position so that object lens
105 focus is on the optimum position that cell 100 to be measured is imaged.
As shown in figure 4, when the focus of object lens 105 is not on solid-liquid interface, shape on CCD/CMOS imaging devices 108
Into the brightness of hot spot be uneven, due to detecting light beam and second comprising the part first that is reflected back in the 3rd Returning beam
Light beam is detected, the first detection light beam and the second detection light beam are asymmetric, therefore the received on two quadrant photodetector 113
The optical signal of three Returning beams power on two quadrants be it is different, two quadrant photodetector 113 formation hot spot it is bright
Degree is also uneven.Light spot image information is sent to controller 109, the basis of controller 109 by two quadrant photodetector 113
The light spot image information that two quadrant photodetector 113 is sent, adjusts the position of object lens 105, makes the focus of object lens 105 in solid-liquid
On interface.When the focus of object lens 105 is on solid-liquid interface, in the light of two quadrants on two quadrant photodetector 113
Signal intensity is identical, and the brightness of the hot spot of formation is uniform in two quadrants.Finally, controller 109 is further according to pre-
If the distance between optimum position and the solid-liquid interface of cell imaging to be measured H, make the focus of object lens 105 in cell to be measured
On the optimum position of 100 imagings.Wherein, during object lens 105 are adjusted, the light formed on CCD/CMOS imaging devices 108
Spot can be observed.The side of object lens is adjusted by using the light spot image information sent according to two quadrant photodetector
Method, controller faster can be adjusted to the position of object lens.
On the basis of above-described embodiment, as shown in figure 5, described device, in addition to：
Second photodetector 114, for receiving the 4th Returning beam, and converts optical signals into the transmission of the second electric signal
To controller 109；Wherein, the first photodetector in the second photodetector 114 and embodiment two can be same light
Electric explorer.
Wherein, controller 109, for according to the optimum position and solid-liquid interface that cell 100 to be measured is imaged in culture box
The distance between, the light spot image information that sends of two quadrant photodetector 113 and the second photodetector 114 send the
Two electric signals, adjust the position of object lens 105, so that the focus of object lens 105 is on the optimum position that cell 100 to be measured is imaged.
PZ axial curves can be formed on second photodetector 114, principle is identical with the first above-mentioned photodetector, no
Tire out again and state.
The embodiment of the present invention three provides a kind of device of auto-focusing, and the device is added on the basis of embodiment one
More optical elements, and to the function of optical element therein, be optimized.The embodiment of the present invention can quickly, accurately
Auto-focusing is realized on ground, solves to shoot multi-pass operation, slow-footed problem of focusing during picture.
Example IV
Fig. 6 is a kind of method flow diagram for auto-focusing that the embodiment of the present invention four is provided；As shown in fig. 6, described side
Method includes：
S601:By laser emission detection light beam, and by fiber coupler to the detection light launched from laser
Shu Jinhang coupling processings.
S602：It is divided into by inclined surface fiber transmission detection light beam and by detection light beam along asymmetric first spy of optical axis
Light-metering beam and the second detection light beam.
S603:Collimation processing is carried out to the first detection light beam and the second detection light beam by collimating element.
S605:Detection light beam is received by object lens, the first detection light beam and the second detection light beam is focused at cell to be measured
Cultivate on the solid-liquid interface in box.
S606：The beam splitter reflected to solid-liquid interface by the first semi-transparent semi-reflecting lens for the first Returning beam and
Second Returning beam.
S607：The first Returning beam is corrected by the first relay lens and focusing.
S608:Receive the first Returning beam to form hot spot by CCD/CMOS imaging devices, and by light spot image information
It is sent to controller.
S609：By controller according to culture box in cell imaging to be measured optimum position and solid-liquid interface between away from
From and CCD/CMOS imaging devices send light spot image information, adjust object lens position so that the focus of object lens is to be measured
On the optimum position of cell imaging.
On the basis of above-described embodiment, as shown in fig. 6, methods described also includes：
It is described by object lens by first detection light beam and second detection light beam focus on cell to be measured before, including：
S604:The first detection light beam and the second detection light beam are reflected by the first semi-transparent semi-reflecting lens, so that first
Detection light beam and the second detection light beam enter object lens.
On the basis of above-described embodiment, the first Returning beam is being received to form light by CCD/CMOS imaging devices
Spot, and light spot image information is sent to after controller, in addition to：
Second Returning beam is received by the first photodetector, and converts optical signals into the first electric signal, and the
One electric signal is sent to controller；
By the controller according to culture box in cell imaging to be measured optimum position and solid-liquid interface between away from
The first electric signal that the light spot image information sent from, CCD/CMOS imaging devices and the first photodetector are sent, adjustment
The position of object lens, so that the focus of object lens is on the optimum position of cell imaging to be measured.
Specific operating process, as shown in fig. 7, comprises：
S701:By laser emission detection light beam, and by fiber coupler to the detection light launched from laser
Shu Jinhang coupling processings.
S702：It is divided into by inclined surface fiber transmission detection light beam and by detection light beam along asymmetric first spy of optical axis
Light-metering beam and the second detection light beam.
S703:Collimation processing is carried out to the first detection light beam and the second detection light beam by collimating element.
S704:The first detection light beam and the second detection light beam are reflected by the first semi-transparent semi-reflecting lens, so that first
Detection light beam and the second detection light beam enter object lens.
S705:Detection light beam is received by object lens, the first detection light beam and the second detection light beam is focused at cell to be measured
Cultivate on the solid-liquid interface in box.
The beam splitter that S706 is reflected to solid-liquid interface by the first semi-transparent semi-reflecting lens for the first Returning beam and
Second Returning beam.
S707：The first Returning beam is corrected by the first relay lens and focusing.
S708:Receive the first Returning beam to form hot spot by CCD/CMOS imaging devices, and by light spot image information
It is sent to controller.
S709：Second Returning beam is received by the first photodetector, and converts optical signals into the first electric signal, and
First electric signal is sent to controller.
S710：By the controller according to culture box in cell imaging to be measured optimum position and solid-liquid interface between
Distance, CCD/CMOS imaging devices send light spot image information and the first photodetector send the first electric signal,
The position of object lens is adjusted, so that the focus of object lens is on the optimum position of cell imaging to be measured.
On the basis of above-described embodiment, standard is being carried out to the first detection light beam and the second detection light beam by collimating element
After straight processing, in addition to：The first detection light beam and the second detection light beam are transmitted by the second semi-transparent semi-reflecting lens.
It is the first Returning beam and the in the beam splitter reflected by the first semi-transparent semi-reflecting lens to solid-liquid interface
After two Returning beams, in addition to：
The second Returning beam is split into the 3rd Returning beam and the 4th Returning beam by the second semi-transparent semi-reflecting lens；
The 3rd Returning beam is corrected and focusing by the second relay lens；
It is used to receive the 3rd Returning beam to form hot spot by two quadrant photodetector, and light spot image information is sent out
Give controller；
By the controller according to culture box in cell imaging to be measured optimum position and solid-liquid interface between away from
From and two quadrant photodetector send light spot image information, adjust object lens position so that the focus of object lens is to be measured
On the optimum position of cell imaging.
Specific operating process as shown in figure 8, including：
S801:Pass through laser emission detection light beam；And by fiber coupler to the detection light launched from laser
Shu Jinhang coupling processings.
S802：It is divided into by inclined surface fiber transmission detection light beam and by detection light beam along asymmetric first spy of optical axis
Light-metering beam and the second detection light beam.
S803:Collimation processing is carried out to the first detection light beam and the second detection light beam by collimating element.
S804：The first detection light beam and the second detection light beam are transmitted by the second semi-transparent semi-reflecting lens.
S805：The first detection light beam and the second detection light beam are reflected by the first semi-transparent semi-reflecting lens, so that first
Detection light beam and the second detection light beam enter object lens.
S806:Detection light beam is received by object lens, the first detection light beam and the second detection light beam is focused at cell to be measured
Cultivate on the solid-liquid interface in box.
S807：The beam splitter reflected to solid-liquid interface by the first semi-transparent semi-reflecting lens for the first Returning beam and
Second Returning beam.
Second Returning beam is split into the 3rd Returning beam and the 4th return by S808 by the second semi-transparent semi-reflecting lens
Light beam；
S809：The 3rd Returning beam is corrected and focusing by the second relay lens.
S810：It is used to receive the 3rd Returning beam by two quadrant photodetector to form hot spot, and by light spot image
Information is sent to controller.
S811：The first Returning beam is corrected by the first relay lens and focusing.
S812:Receive the first Returning beam to form hot spot by CCD/CMOS imaging devices.
S813：By the controller according to culture box in cell imaging to be measured optimum position and solid-liquid interface between
Distance and two quadrant photodetector send light spot image information, adjust object lens position so that the focus of object lens exists
On the optimum position of cell imaging to be measured.
On the basis of above-described embodiment, it is being used to receive the 3rd Returning beam to be formed by two quadrant photodetector
Hot spot, and light spot image information is sent to after controller, in addition to：
4th Returning beam is received by the second photodetector, and converts optical signals into the second electric signal and is sent to control
Device processed；
By the controller according to culture box in cell imaging to be measured optimum position and solid-liquid interface between away from
The second electric signal that the light spot image information sent from, two quadrant photodetector and the second photodetector are sent, adjustment
The position of object lens, so that the focus of object lens is on the optimum position of cell imaging to be measured.
Specific operating process is as shown in figure 9, methods described includes：
S901:Pass through laser emission detection light beam；And by fiber coupler to the detection light launched from laser
Shu Jinhang coupling processings.
S902：It is divided into by inclined surface fiber transmission detection light beam and by detection light beam along asymmetric first spy of optical axis
Light-metering beam and the second detection light beam.
S903:Collimation processing is carried out to the first detection light beam and the second detection light beam by collimating element.
S904：The first detection light beam and the second detection light beam are transmitted by the second semi-transparent semi-reflecting lens.
S905：The first detection light beam and the second detection light beam are reflected by the first semi-transparent semi-reflecting lens, so that first
Detection light beam and the second detection light beam enter object lens.
S906:Detection light beam is received by object lens, the first detection light beam and the second detection light beam is focused at cell to be measured
Cultivate on the solid-liquid interface in box.
S907：The beam splitter reflected to solid-liquid interface by the first semi-transparent semi-reflecting lens for the first Returning beam and
Second Returning beam.
S908：The second Returning beam is split into the 3rd Returning beam and the 4th return by the second semi-transparent semi-reflecting lens
Light beam.
S909：The 3rd Returning beam is corrected and focusing by the second relay lens.
S910：It is used to receive the 3rd Returning beam by two quadrant photodetector to form hot spot, and by light spot image
Information is sent to controller.
S911：4th Returning beam is received by the second photodetector, and converts optical signals into the second electric signal hair
Give controller.
S912：The first Returning beam is corrected by the first relay lens and focusing.
S913:Receive the first Returning beam to form hot spot by CCD/CMOS imaging devices.
S914：By the controller according to culture box in cell imaging to be measured optimum position and solid-liquid interface between
Distance, two quadrant photodetector send light spot image information and the second photodetector send the second electric signal,
The position of object lens is adjusted, so that the focus of object lens is on the optimum position of cell imaging to be measured.
The embodiments of the invention provide a kind of method of auto-focusing, auto-focusing can be quickly and accurately realized, is solved
Shoot multi-pass operation, slow-footed problem of focusing during picture.
Note, above are only presently preferred embodiments of the present invention and institute's application technology principle.It will be appreciated by those skilled in the art that
The invention is not restricted to specific embodiment described here, can carry out for a person skilled in the art it is various it is obvious change,
Readjust and substitute without departing from protection scope of the present invention.Therefore, although the present invention is carried out by above example
It is described in further detail, but the present invention is not limited only to above example, without departing from the inventive concept, also
Other more equivalent embodiments can be included, and the scope of the present invention is determined by scope of the appended claims.
Claims (8)
1. a kind of device of auto-focusing, it is characterised in that including：
Laser, for emission detection light beam；
Fiber coupler, for carrying out coupling processing to the detection light beam launched from laser；
Inclined surface fiber, for transmit detection light beam and will detection light beam be divided into along optical axis it is asymmetric first detection light beam and
Second detection light beam；
Collimating element, for the first detection light beam and the second detection light beam to be carried out into collimation processing；
First semi-transparent semi-reflecting lens, for reflecting the first detection light beam and the second detection light beam, so that the first detection light beam
Enter object lens with the second detection light beam；
Object lens, detect light beam and the second detection light beam for receiving first, the first detection light beam and second is detected light beam convergence
On solid-liquid interface in cell culture case to be measured；
First semi-transparent semi-reflecting lens, it is the first Returning beam and the to be additionally operable to the beam splitter that is reflected to solid-liquid interface
Two Returning beams；Wherein, the light beam that the solid-liquid interface is reflected is the first spy that the solid-liquid interface is reflected
Light-metering beam and the light beam of the second detection light beam formation；
First relay lens, for being corrected and focusing to the first Returning beam；
CCD/CMOS imaging devices, for receiving the first Returning beam to form hot spot, and are sent to control by light spot image information
Device processed；
Controller, for the light spot image information sent according to the CCD/CMOS imaging devices, adjusts the position of the object lens,
So that the focus of the object lens is on the solid-liquid interface, and the optimum position according to default cell imaging to be measured with it is described
The distance between solid-liquid interface, adjusts the object lens, so that the focus of the object lens is in the optimum position of cell imaging to be measured.
2. device according to claim 1, it is characterised in that also include：First photodetector, is returned for receiving second
Light echo beam, and the first electric signal is converted optical signals into, and the first electric signal is sent to controller；
The controller, is imaged for the first electric signal sent according to first photodetector and the CCD/CMOS
The light spot image information that device is sent adjusts the position of the object lens, so that the focus of the object lens is in the solid-liquid interface
The distance between on, and optimum position according to default cell imaging to be measured and the solid-liquid interface, the object lens are adjusted,
So that the focus of the object lens is in the optimum position of cell imaging to be measured.
3. device according to claim 1, it is characterised in that also include：Second semi-transparent semi-reflecting lens, for being visited to described first
Light-metering beam and the second detection light beam are transmitted, and for the second Returning beam to be split into the 3rd Returning beam
With the 4th Returning beam；
Second relay lens, for being corrected and focusing to the 3rd Returning beam；
Two quadrant photodetector, for receiving the 3rd Returning beam to form hot spot, and is sent to control by light spot image information
Device processed；
The controller, for the light spot image information sent according to the two quadrant photodetector, adjusts the object lens
Position, so that the focus of the object lens is on the solid-liquid interface, and according to the optimum position of default cell imaging to be measured
The distance between with the solid-liquid interface, adjust the object lens so that the focus of the object lens in cell imaging to be measured most
Best placement.
4. device according to claim 3, it is characterised in that also include：Second photodetector, is returned for receiving the 4th
Light echo beam, and convert optical signals into the second electric signal and be sent to controller；
The controller, is visited for the second electric signal sent according to second photodetector and the two quadrant photoelectricity
The position for the light spot image information adjustment object lens that device is sent is surveyed, so that the focus of the object lens is in the solid-liquid interface
The distance between on, and optimum position according to default cell imaging to be measured and the solid-liquid interface, the object lens are adjusted,
So that the focus of the object lens is in the optimum position of cell imaging to be measured.
5. a kind of method of automatic focusing, it is characterised in that including：
The detection light beam launched from laser is coupled by laser emission detection light beam, and by fiber coupler
Processing；
By inclined surface fiber transmission detection light beam and will detection light beam be divided into along optical axis it is asymmetric first detection light beam and
Second detection light beam；
First detection light beam and the second detection light beam are carried out by collimation processing by collimating element；
By the first semi-transparent semi-reflecting lens to first detection light beam and second detection light beam reflect so that first detection light beam with
Second detection light beam enters object lens；
First is received by object lens and detects light beam and the second detection light beam, is focused at the first detection light beam and the second detection light beam
On solid-liquid interface in cell culture case to be measured；
The beam splitter reflected by first semi-transparent semi-reflecting lens to solid-liquid interface is the first Returning beam and second
Returning beam；Wherein, the light beam that the solid-liquid interface is reflected is the first detection that the solid-liquid interface is reflected
Light beam and the light beam of the second detection light beam formation；
The first Returning beam is corrected by the first relay lens and focusing；
First Returning beam is received to form hot spot by CCD/CMOS imaging devices, and light spot image information is sent to control
Device；
The light spot image information sent by controller according to the CCD/CMOS imaging devices, adjusts the position of the object lens,
So that the focus of the object lens is on the solid-liquid interface, and by the controller according to default cell imaging to be measured
The distance between optimum position and the solid-liquid interface, adjust the object lens, so that the focus of the object lens is in cell to be measured
The optimum position of imaging.
6. method according to claim 5, it is characterised in that returned described by CCD/CMOS imaging devices reception first
Light spot image information is sent to after controller by light echo beam with forming hot spot, in addition to：
Second Returning beam is received by the first photodetector, and converts optical signals into the first electric signal, and the first electricity
Signal is sent to controller；
The first electric signal sent by the controller according to first photodetector and CCD/CMOS imagings
The light spot image information that device is sent adjusts the position of the object lens, so that the focus of the object lens is in the solid-liquid interface
On, and by the controller according between the optimum position of default cell imaging to be measured and the solid-liquid interface away from
From the adjustment object lens, so that the focus of the object lens is in the optimum position of cell imaging to be measured.
7. method according to claim 5, it is characterised in that it is described by the first semi-transparent semi-reflecting lens to solid-liquid interface
After the beam splitter reflected is the first Returning beam and the second Returning beam, in addition to：
The described first detection light beam and the second detection light beam are transmitted by the second semi-transparent semi-reflecting lens, and by second
Returning beam is split into the 3rd Returning beam and the 4th Returning beam；
The 3rd Returning beam is corrected and focusing by the second relay lens；
It is used to receive the 3rd Returning beam to form hot spot by two quadrant photodetector, and light spot image information is sent to
Controller；
The light spot image information sent by the controller according to the two quadrant photodetector, adjusts the position of the object lens
Put so that the focus of the object lens is on the solid-liquid interface, and by the controller according to default cell to be measured into
The distance between the optimum position of picture and the solid-liquid interface, adjust the object lens, so that the focus of the object lens is to be measured
The optimum position of cell imaging.
8. method according to claim 7, it is characterised in that by two quadrant photodetector be used to receiving the described
Light spot image information is sent to after controller by three Returning beams with forming hot spot, in addition to：
4th Returning beam is received by the second photodetector, and converts optical signals into the second electric signal and is sent to control
Device；
The second electric signal sent by the controller according to second photodetector and the two quadrant photoelectricity are visited
The position for the light spot image information adjustment object lens that device is sent is surveyed, so that the focus of the object lens is in the solid-liquid interface
On, and by the controller according between the optimum position of default cell imaging to be measured and the solid-liquid interface away from
From the adjustment object lens, so that the focus of the object lens is in the optimum position of cell imaging to be measured.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510439170.4A CN104977779B (en) | 2015-07-23 | 2015-07-23 | A kind of device and method of auto-focusing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510439170.4A CN104977779B (en) | 2015-07-23 | 2015-07-23 | A kind of device and method of auto-focusing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104977779A CN104977779A (en) | 2015-10-14 |
| CN104977779B true CN104977779B (en) | 2017-10-20 |
Family
ID=54274417
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510439170.4A Active CN104977779B (en) | 2015-07-23 | 2015-07-23 | A kind of device and method of auto-focusing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104977779B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106772983A (en) * | 2015-11-23 | 2017-05-31 | 大连光耀辉科技有限公司 | A kind of microscope of auto-focusing |
| CN106842472A (en) * | 2017-01-12 | 2017-06-13 | 哈尔滨工业大学 | A kind of method of transmitting/receiving light path focusing |
| CN107065077B (en) * | 2017-03-16 | 2019-11-05 | 精微视达医疗科技（武汉）有限公司 | A kind of fiber bundle coupling device of auto-focusing positioning |
| CN111913187A (en) * | 2020-08-11 | 2020-11-10 | 生物岛实验室 | Distance measuring method and microscopic distance measuring device |
| CN114755770B (en) * | 2022-05-16 | 2023-07-28 | 江苏镭创高科光电科技有限公司 | Automatic dimming system and dimming method for laser coupling optical fiber |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202177564U (en) * | 2011-07-08 | 2012-03-28 | 无锡荣兴科技有限公司 | Blood cell analyzer adopting blue semiconductor laser |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2606662B2 (en) * | 1993-12-24 | 1997-05-07 | 日本電気株式会社 | Focus position detector |
| JP3754164B2 (en) * | 1997-02-27 | 2006-03-08 | 株式会社東芝 | Sample inspection equipment |
| JPH10307252A (en) * | 1997-05-07 | 1998-11-17 | Hitachi Denshi Ltd | Automatic focusing optical television camera microscope |
| GB0321918D0 (en) * | 2003-09-19 | 2003-10-22 | Aoti Operating Co Inc | Focusing system and method |
| JP2010091809A (en) * | 2008-10-08 | 2010-04-22 | Nikon Corp | Microscope apparatus |
-
2015
- 2015-07-23 CN CN201510439170.4A patent/CN104977779B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202177564U (en) * | 2011-07-08 | 2012-03-28 | 无锡荣兴科技有限公司 | Blood cell analyzer adopting blue semiconductor laser |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104977779A (en) | 2015-10-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104977779B (en) | A kind of device and method of auto-focusing | |
| KR100477803B1 (en) | Optical alignment apparatus and method by using visual optical source and image | |
| JP6621580B2 (en) | Laser measurement system and method | |
| US20140176933A1 (en) | Light detecting and ranging sensing apparatus and methods | |
| US20140231647A1 (en) | Compact fiber-based scanning laser detection and ranging system | |
| CN105910712B (en) | Five-channel self-adaption two-dimensional temperature field measuring apparatus and its measurement method | |
| CN201293684Y (en) | Three-way real time temperature measurement thermal imager | |
| JP7419394B2 (en) | LIDAR system with mode field expander | |
| CN105334028B (en) | A kind of scaling method that dual-beam synthesis precision and pointing accuracy are improved using simple detector synthesis far field | |
| CN108955563A (en) | Combined type continuous frequency modulation laser radar apparatus and measurement method for topography scan | |
| CN105911557A (en) | Frequency modulated continuous wave laser ranging light beam automatic focusing device based on liquid lens | |
| EP3036577B1 (en) | Autofocus apparatus | |
| CN110471083A (en) | A kind of laser three-dimensional imaging device and method of fore-and-aft distance | |
| CN103620340A (en) | Coupled multi-wavelength confocal systems for distance measurements | |
| CN104848805B (en) | A kind of two waveband synthesis light beam detection process and device based on monochromatic light wedge | |
| CN104991402B (en) | A kind of device and method of auto-focusing | |
| US9134522B2 (en) | Autofocus apparatus | |
| US4115005A (en) | Method for optically measuring a roughness profile of surface | |
| CN106842534B (en) | Automatic focusing microscope based on double CCD and measuring method | |
| JP2002296018A (en) | Three-dimensional shape measuring instrument | |
| CN113678043B (en) | Injection of radiation beams into optical fibers | |
| CN101751148B (en) | Capturing device and capturing method for non-deformable light spots | |
| US11344970B2 (en) | Optical device and method for detecting the drift of a light beam | |
| CN112462386A (en) | Modular continuous frequency modulation laser radar's two-dimensional topography scanning device | |
| CN213179864U (en) | Multi-degree-of-freedom measuring device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |