CN104977779A - Automatic focusing device and method thereof - Google Patents
Automatic focusing device and method thereof Download PDFInfo
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- CN104977779A CN104977779A CN201510439170.4A CN201510439170A CN104977779A CN 104977779 A CN104977779 A CN 104977779A CN 201510439170 A CN201510439170 A CN 201510439170A CN 104977779 A CN104977779 A CN 104977779A
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Abstract
The present invention discloses an automatic focusing device and method thereof. According to the device, the position of an object lens is adjusted through a laser, a fiber coupler, an inclined end face optical fiber, a collimating element, an objective lens, a first transflective lens, a first relay lens, a CCD/CMOS imaging device and a controller such that the focus of the object lens can be rapidly adjusted to the optimal position of the imaging of a cell to be detected. According to the embodiment of the present invention, the automatic focusing can be rapidly and accurately realized, and the problems of multiple operations and slow focusing speed in the photo taking process are solved.
Description
Technical field
The present invention relates to technical field of optical detection, particularly relate to a kind of device and method of auto-focusing.
Background technology
In the research field of cell, the real-time monitored of cell is very important link, and form, division etc. comprising observation cell study the growth of cell and the activity situation of cell.
In the lab, the cell being used for studying generally grows on the bottom surface of cultivating box, and cell is in continuous motion, wherein cell has refringence with the bottom surface of cultivating box, also there is refringence outside air and cultivation box bottom surface, the two all easily causes reflection, so when observing cell by cell take, capture apparatus carries out auto-focusing to be needed accurately and fast.
At present, the method for capture apparatus auto-focusing mainly two kinds, one is take plurality of pictures continuously, by image procossing, contrasts the acutance of every pictures, brightness and contrast, chooses the pictures that shooting effect is best; The second is by range sensor, measures the distance between object and camera lens, and repeatedly optimum position is found in range finding.But these two kinds of methods all need multi-pass operations, focusing speed is slower.
Summary of the invention
The object of the present invention is to provide a kind of device and method of auto-focusing, auto-focusing can be realized quickly and accurately, solve multi-pass operations in pictures taken process, slow-footed problem of focusing.
For reaching this object, the present invention by the following technical solutions:
First aspect, the invention provides a kind of device of auto-focusing, comprising:
Laser instrument, for emission detection light beam;
Fiber coupler, for carrying out coupling processing to the detecting light beam launched from laser instrument;
Inclined surface fiber, for transmitting detecting light beam and being divided into by detecting light beam along asymmetric first detecting light beam of optical axis and the second detecting light beam;
Collimating element, for carrying out collimation process by the first detecting light beam and the second detecting light beam;
Object lens, for receiving detecting light beam, make the first detecting light beam and the second detecting light beam be focused on the solid-liquid interface in cell culture case to be measured;
First semi-transparent semi-reflecting lens, the beam splitter for reflecting solid-liquid interface is the first Returning beam and the second Returning beam;
First relay lens, for correcting the first Returning beam and focusing on process;
CCD/CMOS imaging device, for receiving the first Returning beam to form hot spot, and sends to controller by light spot image information;
Controller, for the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and CCD/CMOS imaging device, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
Second aspect, the embodiment of the present invention additionally provides a kind of method of auto-focusing, comprising:
By laser instrument emission detection light beam, and by fiber coupler, coupling processing is carried out to the detecting light beam launched from laser instrument;
Transmit detecting light beam by inclined surface fiber and detecting light beam be divided into along asymmetric first detecting light beam of optical axis and the second detecting light beam;
By collimating element, the first detecting light beam and the second detecting light beam are carried out collimation process;
Receive detecting light beam by object lens, make the first detecting light beam and the second detecting light beam be focused on the solid-liquid interface in cell culture case to be measured;
Be the first Returning beam and the second Returning beam by the first semi-transparent semi-reflecting lens to the beam splitter that solid-liquid interface reflects;
By the first relay lens the first Returning beam corrected and focus on process;
Receive the first Returning beam to form hot spot by CCD/CMOS imaging device, and light spot image information is sent to controller;
By the light spot image information that controller sends according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and CCD/CMOS imaging device, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
Beneficial effect of the present invention is: the device and method of a kind of auto-focusing of the present invention, is divided into along asymmetric first detecting light beam of optical axis and the second detecting light beam by sloping optical fiber by detecting light beam; By collimating element, collimation process is carried out to the first detecting light beam and the second detecting light beam; By object lens, the first detecting light beam and the second detecting light beam are focused on the solid-liquid interface in cell culture case to be measured; The beam splitter reflected by solid-liquid interface by the first semi-transparent semi-reflecting lens is the first Returning beam and the second Returning beam; The first Returning beam is received to form hot spot by CCD/CMOS imaging device, and light spot image information is sent to controller, the light spot image information that controller sends according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and CCD/CMOS imaging device, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.The device of auto-focusing of the present invention can realize auto-focusing quickly and accurately, solves multi-pass operations in pictures taken process, slow-footed problem of focusing.
Accompanying drawing explanation
By reading the detailed description done non-limiting example done with reference to the following drawings, other features, objects and advantages of the present invention will become more obvious:
The apparatus structure block diagram of a kind of auto-focusing that Fig. 1 provides for the embodiment of the present invention one;
The apparatus structure block diagram of a kind of auto-focusing that Fig. 2 provides for the embodiment of the present invention two;
The PZ axial curve figure that the first photodetector that Fig. 3 provides for the embodiment of the present invention two is formed;
The apparatus structure block diagram of a kind of auto-focusing that Fig. 4 provides for the embodiment of the present invention three;
The apparatus structure block diagram of the another kind of auto-focusing that Fig. 5 provides for the embodiment of the present invention three;
The method flow diagram of the first auto-focusing that Fig. 6 provides for the embodiment of the present invention four;
The method flow diagram of the second auto-focusing that Fig. 7 provides for the embodiment of the present invention four;
The method flow diagram of the third auto-focusing that Fig. 8 provides for the embodiment of the present invention four;
The method flow diagram of the 4th kind of auto-focusing that Fig. 9 provides for the embodiment of the present invention four.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail.Be understandable that, specific embodiment described herein is only for explaining the present invention, but not limitation of the invention.It also should be noted that, for convenience of description, illustrate only part related to the present invention in accompanying drawing but not full content.
Embodiment one
The apparatus structure block diagram of a kind of auto-focusing that Fig. 1 provides for the embodiment of the present invention one, as shown in Figure 1, described device comprises: laser instrument 101, fiber coupler 102, inclined surface fiber 103, collimating element 104, object lens 105, first semi-transparent semi-reflecting lens 106, first relay lens 107, CCD/CMOS imaging device 108 and controller 109.
Wherein, laser instrument 101, for emission detection light beam;
Fiber coupler 102, for carrying out coupling processing to the detecting light beam launched from laser instrument 101.
Inclined surface fiber 103, for transmitting detecting light beam and being divided into by detecting light beam along asymmetric first detecting light beam of optical axis and the second detecting light beam; Wherein, inclined surface fiber 103 is single-mode fiber, adopts FC/APC fiber connector, makes the exiting surface of single-mode fiber be inclined-plane.Asymmetric (quantity of the light of the first detecting light beam and the second detecting light beam is different) by exiting surface first detecting light beam out of inclined surface fiber 103 and the second detecting light beam along optical axis.
Collimating element 104, for carrying out collimation process by the first detecting light beam and the second detecting light beam; Wherein, collimating element is preferably collimating mirror or beam expanding lens.
Object lens 105, for receiving detecting light beam, making the first detecting light beam and the second detecting light beam be focused at cell 100 to be measured and cultivating on the solid-liquid interface in box;
First semi-transparent semi-reflecting lens 106, the beam splitter for reflecting solid-liquid interface is the first Returning beam and the second Returning beam.Wherein, described first semi-transparent semi-reflecting lens 106 can also be preferably optical filter, polarization light splitting piece or spectroscope etc.; Contain respectively in first Returning beam and the second Returning beam by the first detecting light beam of half of reflecting and the second detecting light beam of half.
First relay lens 107, for correcting the first Returning beam and focusing on process;
CCD/CMOS imaging device 108, for receiving the first Returning beam to form hot spot, and sends to controller 109 by light spot image information;
Controller 109, for the light spot image information sent according to the distance in the cultivation box preset between the optimum position of cell imaging to be measured and solid-liquid interface and CCD/CMOS imaging device 108, the position of adjustment object lens 105, to make the focus of object lens 105 on the optimum position of cell 100 to be measured imaging.Wherein, cultivate solid-liquid interface in box and can reflect the first detecting light beam and the second detecting light beam, have certain distance H between the optimum position of cell 100 to be measured imaging and solid-liquid interface, before testing, need H to set.
On the basis of above-described embodiment, described first semi-transparent semi-reflecting lens 106, also for reflecting the first detecting light beam and the second detecting light beam, enters object lens 105 to make the first detecting light beam and the second detecting light beam.
The said apparatus course of work is as follows: after laser instrument 101 emission detection light beam, be coupled by fiber coupler 102, transmits detecting light beam and be divided into by detecting light beam along asymmetric first detecting light beam of optical axis and the second detecting light beam by inclined surface fiber 103; Through collimating element 104, first detecting light beam and the second detecting light beam are carried out collimation process, enter object lens 105 through the first semi-transparent semi-reflecting lens 106 reflection.
After entering object lens 105, the first detecting light beam and the second detecting light beam is made to be focused on the solid-liquid interface of cultivation box, solid-liquid interface reflects the first detecting light beam and the second detecting light beam respectively, the light beam be reflected back becomes the first Returning beam and the second Returning beam through the first semi-transparent semi-reflecting lens 106 beam splitting, first Returning beam is received by CCD/CMOS imaging device 108, form hot spot, and light spot image information is sent to controller 109.
Finally, controller 109 is according to the light spot image information received, the position of adjustment object lens 105, make the focus of object lens 105 on solid-liquid interface, and according to the distance H between the optimum position of cell imaging to be measured of presetting and solid-liquid interface, adjustment object lens 105, make the focus of object lens 105 in the optimum position of cell imaging to be measured.Wherein, when the focus of object lens is not on solid-liquid interface, CCD/CMOS imaging device is formed the hot spot of circular brightness irregularities, the first detecting light beam and the second detecting light beam that are reflected back is comprised in the first Returning beam due to formation hot spot, the exiting surface of inclined surface fiber is inclined-plane, cause the first detecting light beam and the second detecting light beam asymmetric along optical axis, cause the uneven of hot spot brightness.When the focus of object lens is on solid-liquid interface, at the hot spot brightness uniformity that CCD/CMOS imaging device is formed.
On the basis of above-described embodiment, above-mentioned hot spot can also be formed by other forms.Such as, inclined surface fiber is replaced to the optical fiber that exiting surface is flat mouth, but the light beam after collimating element collimation is blocked a part, make the light beam after collimation asymmetric.
In the present embodiment, owing to have employed inclined surface fiber, make the device internal structure of described auto-focusing simple, easy to operate, and the cost of manufacture of described device can be reduced.
Embodiments provide a kind of device of auto-focusing, this device is by laser instrument, fiber coupler, inclined surface fiber, collimating element, object lens, the first semi-transparent semi-reflecting lens, the first relay lens, CCD/CMOS imaging device and controller, adjust the position of object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.The embodiment of the present invention can realize auto-focusing quickly and accurately, solves multi-pass operations in pictures taken process, slow-footed problem of focusing.
Embodiment two
The present embodiment is similar to the structure of embodiment one, and difference is only that the device of the auto-focusing of the present embodiment also comprises the first photodetector, the apparatus structure block diagram of a kind of auto-focusing that Fig. 2 provides for the embodiment of the present invention two; As shown in Figure 2, described device also comprises:
First photodetector 110, for receiving the second Returning beam, and converts light signal to first electric signal, and the first electric signal is sent to controller 109;
Wherein, in the present embodiment, controller 109, for the first electric signal that the light spot image information that sends according to the distance of cultivating between the optimum position of cell 100 to be measured imaging in box and solid-liquid interface, CCD/CMOS imaging device 108 and the first photodetector 110 send, the position of adjustment object lens 105, to make the focus of object lens 105 on the optimum position of cell 100 to be measured imaging.
On the basis of above-described embodiment, as shown in Figure 2, inclined surface fiber is superfine single-mode fiber, due to the principle of copolymerization Jiao, when the focus of object lens 105 is positioned at solid-liquid interface or distance solid-liquid interface is very near when, first photodetector 110 just can detect the second Returning beam, first photodetector 110 generates PZ axial curve figure, whether evenly observe the hot spot brightness formed in CCD/COMS imaging device 108 simultaneously, controller 109 obtains and records the peak value of the PZ axial curve that the first photodetector 110 detects, the position of further adjustment object lens 105.Fig. 3 is the PZ axial curve figure that the first photodetector is formed, as shown in Figure 3, Z axis represents the position of object lens, and the distance between Z1 and Z2 is very little, Z1 and Z2 represents the focus closely solid-liquid interface of object lens, and Z0 represents that the focus of object lens is positioned at solid-liquid interface.
After the focus of object lens is adjusted on solid-liquid interface by controller, according to the distance H between the optimum position of cell imaging to be measured of presetting and solid-liquid interface, object lens is adjusted again, makes the focus of object lens on the optimum position of cell imaging to be measured.
The embodiment of the present invention two provides a kind of device of auto-focusing, this device adds the first photodetector on the basis of embodiment one, the light spot image information that controller can cultivate distance between the optimum position of cell imaging to be measured in box and solid-liquid interface, CCD/CMOS imaging device sends and the first electric signal that the first photodetector sends, the position of adjustment object lens.The embodiment of the present invention can realize auto-focusing quickly and accurately, solves multi-pass operations in pictures taken process, slow-footed problem of focusing.
Embodiment three
The similar of the present embodiment three and embodiment one, difference is only the device adding one group of independent shaping hot spot on the basis of embodiment one, namely the second semi-transparent semi-reflecting lens, the second relay lens and two quadrant photodetector is added, Fig. 4 is the device of a kind of auto-focusing that the embodiment of the present invention provides, as shown in Figure 4, described device, also comprises:
Second semi-transparent semi-reflecting lens 111, is beamed into the 3rd Returning beam and the 4th Returning beam for being carried out by the second Returning beam; Wherein, the second semi-transparent semi-reflecting lens 111 can also be preferably optical filter, polarization light splitting piece or spectroscope etc.
Second relay lens 112, for focusing on and correction process the 3rd Returning beam;
Two quadrant photodetector 113, for receiving the 3rd Returning beam to form hot spot, and sends to controller 109 by light spot image information; Wherein, two chips are contained in two quadrant photodetector.
Wherein, controller 109, for the light spot image information sent according to the distance of cultivating between the optimum position of cell 100 to be measured imaging in box and solid-liquid interface and two quadrant photodetector 113, the position of adjustment object lens, to make the focus of object lens 105 on the optimum position of cell 100 to be measured imaging.
As shown in Figure 4, when the focus of object lens 105 is not on solid-liquid interface, the brightness of the hot spot that CCD/CMOS imaging device 108 is formed is uneven, owing to comprising part first detecting light beam and the second detecting light beam that are reflected back in the 3rd Returning beam, first detecting light beam and the second detecting light beam are asymmetric, therefore light signal power on two quadrants of the 3rd Returning beam that two quadrant photodetector 113 receives is different, the brightness of the hot spot that two quadrant photodetector 113 is formed also is uneven.Light spot image information is sent to controller 109 by two quadrant photodetector 113, the light spot image information that controller 109 sends according to two quadrant photodetector 113, and the position of adjustment object lens 105, makes the focus of object lens 105 on solid-liquid interface.When the focus of object lens 105 is on solid-liquid interface, two quadrant photodetector 113 is identical at the light signal strength of two quadrants, and the brightness of the hot spot of formation is uniform in two quadrants.Finally, controller 109 according to the distance H between the optimum position of cell imaging to be measured of presetting and solid-liquid interface, makes the focus of object lens 105 on the optimum position of cell 100 to be measured imaging again.Wherein, in the process that object lens 105 adjust, the hot spot that CCD/CMOS imaging device 108 is formed can be observed.Carry out the method adjusting object lens by adopting the light spot image information sent according to two quadrant photodetector, controller can adjust the position of object lens faster.
On the basis of above-described embodiment, as shown in Figure 5, described device, also comprises:
Second photodetector 114, for receiving the 4th Returning beam, and converts the second electric signal to and sends to controller 109 by light signal; Wherein, the first photodetector in the second photodetector 114 and embodiment two can be same photodetector.
Wherein, controller 109, for the second electric signal that the light spot image information that sends according to the distance of cultivating between the optimum position of cell 100 to be measured imaging in box and solid-liquid interface, two quadrant photodetector 113 and the second photodetector 114 send, the position of adjustment object lens 105, to make the focus of object lens 105 on the optimum position of cell 100 to be measured imaging.
Second photodetector 114 can form PZ axial curve, and principle is identical with the first above-mentioned photodetector, is not repeated.
The embodiment of the present invention three provides a kind of device of auto-focusing, and this device adds more optical element on the basis of embodiment one, and the function to optical element wherein, be optimized.The embodiment of the present invention can realize auto-focusing quickly and accurately, solves multi-pass operations in pictures taken process, slow-footed problem of focusing.
Embodiment four
Fig. 6 is the method flow diagram of a kind of auto-focusing that the embodiment of the present invention four provides; As shown in Figure 6, described method comprises:
S601: by laser instrument emission detection light beam, and by fiber coupler, coupling processing is carried out to the detecting light beam launched from laser instrument.
S602: transmit detecting light beam by inclined surface fiber and detecting light beam be divided into along asymmetric first detecting light beam of optical axis and the second detecting light beam.
S603: collimation process is carried out to the first detecting light beam and the second detecting light beam by collimating element.
S605: receive detecting light beam by object lens, makes the first detecting light beam and the second detecting light beam be focused on the solid-liquid interface in cell culture case to be measured.
S606: be the first Returning beam and the second Returning beam to the beam splitter that solid-liquid interface reflects by the first semi-transparent semi-reflecting lens.
S607: by the first relay lens the first Returning beam corrected and focus on process.
S608: receive the first Returning beam to form hot spot by CCD/CMOS imaging device, and light spot image information is sent to controller.
S609: the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and CCD/CMOS imaging device by controller, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
On the basis of above-described embodiment, as shown in Figure 6, described method also comprises:
Described by object lens, the first detecting light beam and the second detecting light beam are focused on cell to be measured before, comprising:
S604: reflected the first detecting light beam and the second detecting light beam by the first semi-transparent semi-reflecting lens, enters object lens to make the first detecting light beam and the second detecting light beam.
On the basis of above-described embodiment, received the first Returning beam by CCD/CMOS imaging device to form hot spot, and after light spot image information is sent to controller, also comprising:
By the first photoelectric detector second Returning beam, and convert light signal to first electric signal, and the first electric signal is sent to controller;
The first electric signal that the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface, CCD/CMOS imaging device by described controller and the first photodetector are sent, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
Concrete operating process, as shown in Figure 7, comprising:
S701: by laser instrument emission detection light beam, and by fiber coupler, coupling processing is carried out to the detecting light beam launched from laser instrument.
S702: transmit detecting light beam by inclined surface fiber and detecting light beam be divided into along asymmetric first detecting light beam of optical axis and the second detecting light beam.
S703: collimation process is carried out to the first detecting light beam and the second detecting light beam by collimating element.
S704: reflected the first detecting light beam and the second detecting light beam by the first semi-transparent semi-reflecting lens, enters object lens to make the first detecting light beam and the second detecting light beam.
S705: receive detecting light beam by object lens, makes the first detecting light beam and the second detecting light beam be focused on the solid-liquid interface in cell culture case to be measured.
S706 is the first Returning beam and the second Returning beam by the first semi-transparent semi-reflecting lens to the beam splitter that solid-liquid interface reflects.
S707: by the first relay lens the first Returning beam corrected and focus on process.
S708: receive the first Returning beam to form hot spot by CCD/CMOS imaging device, and light spot image information is sent to controller.
S709: by the first photoelectric detector second Returning beam, and convert light signal to first electric signal, and the first electric signal is sent to controller.
S710: the first electric signal that the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface, CCD/CMOS imaging device by described controller and the first photodetector are sent, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
On the basis of above-described embodiment, after by collimating element the first detecting light beam and the second detecting light beam being carried out to collimation process, also comprise: by the second semi-transparent semi-reflecting lens, transmission is carried out to the first detecting light beam and the second detecting light beam.
After the beam splitter reflected solid-liquid interface by the first semi-transparent semi-reflecting lens is the first Returning beam and the second Returning beam, also comprise:
By the second semi-transparent semi-reflecting lens, the second Returning beam is carried out being beamed into the 3rd Returning beam and the 4th Returning beam;
By the second relay lens, the 3rd Returning beam is corrected and focuses on process;
By two quadrant photodetector for receiving the 3rd Returning beam to form hot spot, and light spot image information is sent to controller;
By the light spot image information that described controller sends according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and two quadrant photodetector, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
Concrete operating process as shown in Figure 8, comprising:
S801: by laser instrument emission detection light beam; And by fiber coupler, coupling processing is carried out to the detecting light beam launched from laser instrument.
S802: transmit detecting light beam by inclined surface fiber and detecting light beam be divided into along asymmetric first detecting light beam of optical axis and the second detecting light beam.
S803: collimation process is carried out to the first detecting light beam and the second detecting light beam by collimating element.
S804: transmission is carried out to the first detecting light beam and the second detecting light beam by the second semi-transparent semi-reflecting lens.
S805: reflected the first detecting light beam and the second detecting light beam by the first semi-transparent semi-reflecting lens, enters object lens to make the first detecting light beam and the second detecting light beam.
S806: receive detecting light beam by object lens, makes the first detecting light beam and the second detecting light beam be focused on the solid-liquid interface in cell culture case to be measured.
S807: be the first Returning beam and the second Returning beam to the beam splitter that solid-liquid interface reflects by the first semi-transparent semi-reflecting lens.
Second Returning beam is undertaken being beamed into the 3rd Returning beam and the 4th Returning beam by the second semi-transparent semi-reflecting lens by S808;
S809: the 3rd Returning beam is corrected and focuses on process by the second relay lens.
S810: by two quadrant photodetector for receiving the 3rd Returning beam to form hot spot, and light spot image information is sent to controller.
S811: by the first relay lens the first Returning beam corrected and focus on process.
S812: receive the first Returning beam to form hot spot by CCD/CMOS imaging device.
S813: the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and two quadrant photodetector by described controller, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
On the basis of above-described embodiment, by two quadrant photodetector for receiving the 3rd Returning beam to form hot spot, and after light spot image information is sent to controller, also to comprise:
By the second photoelectric detector the 4th Returning beam, and light signal is converted to the second electric signal and send to controller;
The second electric signal that the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface, two quadrant photodetector by described controller and the second photodetector are sent, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
As shown in Figure 9, described method comprises concrete operating process:
S901: by laser instrument emission detection light beam; And by fiber coupler, coupling processing is carried out to the detecting light beam launched from laser instrument.
S902: transmit detecting light beam by inclined surface fiber and detecting light beam be divided into along asymmetric first detecting light beam of optical axis and the second detecting light beam.
S903: collimation process is carried out to the first detecting light beam and the second detecting light beam by collimating element.
S904: transmission is carried out to the first detecting light beam and the second detecting light beam by the second semi-transparent semi-reflecting lens.
S905: reflected the first detecting light beam and the second detecting light beam by the first semi-transparent semi-reflecting lens, enters object lens to make the first detecting light beam and the second detecting light beam.
S906: receive detecting light beam by object lens, makes the first detecting light beam and the second detecting light beam be focused on the solid-liquid interface in cell culture case to be measured.
S907: be the first Returning beam and the second Returning beam to the beam splitter that solid-liquid interface reflects by the first semi-transparent semi-reflecting lens.
S908: the second Returning beam is carried out being beamed into the 3rd Returning beam and the 4th Returning beam by the second semi-transparent semi-reflecting lens.
S909: the 3rd Returning beam is corrected and focuses on process by the second relay lens.
S910: by two quadrant photodetector for receiving the 3rd Returning beam to form hot spot, and light spot image information is sent to controller.
S911: by the second photoelectric detector the 4th Returning beam, and light signal is converted to the second electric signal and send to controller.
S912: by the first relay lens the first Returning beam corrected and focus on process.
S913: receive the first Returning beam to form hot spot by CCD/CMOS imaging device.
S914: the second electric signal that the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface, two quadrant photodetector by described controller and the second photodetector are sent, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
Embodiments provide a kind of method of auto-focusing, auto-focusing can be realized quickly and accurately, solve multi-pass operations in pictures taken process, slow-footed problem of focusing.
Note, above are only preferred embodiment of the present invention and institute's application technology principle.Skilled person in the art will appreciate that and the invention is not restricted to specific embodiment described here, various obvious change can be carried out for a person skilled in the art, readjust and substitute and can not protection scope of the present invention be departed from.Therefore, although be described in further detail invention has been by above embodiment, the present invention is not limited only to above embodiment, when not departing from the present invention's design, can also comprise other Equivalent embodiments more, and scope of the present invention is determined by appended right.
Claims (10)
1. a device for auto-focusing, is characterized in that, comprising:
Laser instrument, for emission detection light beam;
Fiber coupler, for carrying out coupling processing to the detecting light beam launched from laser instrument;
Inclined surface fiber, for transmitting detecting light beam and being divided into by detecting light beam along asymmetric first detecting light beam of optical axis and the second detecting light beam;
Collimating element, for carrying out collimation process by the first detecting light beam and the second detecting light beam;
Object lens, for receiving detecting light beam, make the first detecting light beam and the second detecting light beam be focused on the solid-liquid interface in cell culture case to be measured;
First semi-transparent semi-reflecting lens, the beam splitter for reflecting solid-liquid interface is the first Returning beam and the second Returning beam;
First relay lens, for correcting the first Returning beam and focusing on process;
CCD/CMOS imaging device, for receiving the first Returning beam to form hot spot, and sends to controller by light spot image information;
Controller, for the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and CCD/CMOS imaging device, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
2. device according to claim 1, is characterized in that, described first semi-transparent semi-reflecting lens, also for reflecting the first detecting light beam and the second detecting light beam, enters object lens to make the first detecting light beam and the second detecting light beam.
3. device according to claim 1, is characterized in that, also comprises: the first photodetector, for receiving the second Returning beam, and converts light signal to first electric signal, and the first electric signal is sent to controller;
Described controller, for the first electric signal that the light spot image information that sends according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface, CCD/CMOS imaging device and the first photodetector send, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
4. device according to claim 1, is characterized in that, also comprises: the second semi-transparent semi-reflecting lens, being beamed into the 3rd Returning beam and the 4th Returning beam for being carried out by the second Returning beam;
Second relay lens, for correcting the 3rd Returning beam and focus on process;
Two quadrant photodetector, for receiving the 3rd Returning beam to form hot spot, and sends to controller by light spot image information;
Described controller, for the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and two quadrant photodetector, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
5. want 4 devices asking described according to right, it is characterized in that, also comprise: the second photodetector, for receiving the 4th Returning beam, and light signal is converted to the second electric signal and send to controller;
Described controller, for the second electric signal that the light spot image information that sends according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface, two quadrant photodetector and the second photodetector send, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
6. a method for automatic focusing, is characterized in that, comprising:
By laser instrument emission detection light beam, and by fiber coupler, coupling processing is carried out to the detecting light beam launched from laser instrument;
Transmit detecting light beam by inclined surface fiber and detecting light beam be divided into along asymmetric first detecting light beam of optical axis and the second detecting light beam;
By collimating element, the first detecting light beam and the second detecting light beam are carried out collimation process;
Receive detecting light beam by object lens, make the first detecting light beam and the second detecting light beam be focused on the solid-liquid interface in cell culture case to be measured;
Be the first Returning beam and the second Returning beam by the first semi-transparent semi-reflecting lens to the beam splitter that solid-liquid interface reflects;
By the first relay lens the first Returning beam corrected and focus on process;
Receive the first Returning beam to form hot spot by CCD/CMOS imaging device, and light spot image information is sent to controller;
By the light spot image information that controller sends according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and CCD/CMOS imaging device, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
7. method according to claim 6, is characterized in that, receives detecting light beam described by object lens, before on the solid-liquid interface making the first detecting light beam and the second detecting light beam be focused in cell culture case to be measured, also comprises:
By described first semi-transparent semi-reflecting lens, the first detecting light beam and the second detecting light beam are reflected, enter object lens to make the first detecting light beam and the second detecting light beam.
8. method according to claim 6, is characterized in that, receives the first Returning beam to form hot spot, and after light spot image information is sent to controller, also comprise described by CCD/CMOS imaging device:
By the first photoelectric detector second Returning beam, and convert light signal to first electric signal, and the first electric signal is sent to controller;
The first electric signal that the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface, CCD/CMOS imaging device by described controller and the first photodetector are sent, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
9. method according to claim 6, is characterized in that, after the described beam splitter reflected solid-liquid interface by the first semi-transparent semi-reflecting lens is the first Returning beam and the second Returning beam, also comprises:
By the second semi-transparent semi-reflecting lens, the second Returning beam is carried out being beamed into the 3rd Returning beam and the 4th Returning beam;
By the second relay lens, the 3rd Returning beam is corrected and focuses on process;
By two quadrant photodetector for receiving the 3rd Returning beam to form hot spot, and light spot image information is sent to controller;
By the light spot image information that described controller sends according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface and two quadrant photodetector, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
10. method according to claim 9, is characterized in that, described by two quadrant photodetector for receiving the 3rd Returning beam to form hot spot, and after light spot image information is sent to controller, also to comprise:
By the second photoelectric detector the 4th Returning beam, and light signal is converted to the second electric signal and send to controller;
The second electric signal that the light spot image information sent according to the distance of cultivating between the optimum position of cell imaging to be measured in box and solid-liquid interface, two quadrant photodetector by described controller and the second photodetector are sent, the position of adjustment object lens, to make the focus of object lens on the optimum position of cell imaging to be measured.
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