CN104971124B - A kind of new application of pharmaceutical composition - Google Patents
A kind of new application of pharmaceutical composition Download PDFInfo
- Publication number
- CN104971124B CN104971124B CN201410142650.XA CN201410142650A CN104971124B CN 104971124 B CN104971124 B CN 104971124B CN 201410142650 A CN201410142650 A CN 201410142650A CN 104971124 B CN104971124 B CN 104971124B
- Authority
- CN
- China
- Prior art keywords
- parts
- drug
- group
- disease
- chicken
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 17
- 239000003814 drug Substances 0.000 claims abstract description 44
- 229940079593 drug Drugs 0.000 claims abstract description 38
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 36
- 201000010099 disease Diseases 0.000 claims abstract description 34
- 230000002265 prevention Effects 0.000 claims abstract description 26
- 238000002360 preparation method Methods 0.000 claims abstract description 21
- 235000002991 Coptis groenlandica Nutrition 0.000 claims abstract description 12
- 241000607683 Salmonella enterica subsp. enterica serovar Pullorum Species 0.000 claims abstract description 11
- 239000000126 substance Substances 0.000 claims abstract description 8
- 241000218202 Coptis Species 0.000 claims abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 235000019441 ethanol Nutrition 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 6
- 239000003960 organic solvent Substances 0.000 claims description 3
- 230000000295 complement effect Effects 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims description 2
- 125000005909 ethyl alcohol group Chemical group 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 41
- 206010039438 Salmonella Infections Diseases 0.000 abstract description 12
- 206010039447 salmonellosis Diseases 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 9
- 229940088710 antibiotic agent Drugs 0.000 abstract description 7
- 230000003115 biocidal effect Effects 0.000 abstract description 5
- 239000003242 anti bacterial agent Substances 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 3
- 230000000857 drug effect Effects 0.000 abstract description 2
- 241000287828 Gallus gallus Species 0.000 description 44
- 235000013330 chicken meat Nutrition 0.000 description 44
- 208000015181 infectious disease Diseases 0.000 description 26
- 238000012360 testing method Methods 0.000 description 23
- 241000607142 Salmonella Species 0.000 description 22
- 210000001519 tissue Anatomy 0.000 description 20
- 210000000936 intestine Anatomy 0.000 description 19
- 210000004185 liver Anatomy 0.000 description 19
- 230000034994 death Effects 0.000 description 18
- 241000252983 Caecum Species 0.000 description 15
- 241001465754 Metazoa Species 0.000 description 15
- 210000004534 cecum Anatomy 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 14
- 244000247747 Coptis groenlandica Species 0.000 description 10
- 208000032843 Hemorrhage Diseases 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 230000008595 infiltration Effects 0.000 description 10
- 238000001764 infiltration Methods 0.000 description 10
- 210000004072 lung Anatomy 0.000 description 10
- 238000000926 separation method Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 210000002216 heart Anatomy 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 206010012735 Diarrhoea Diseases 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 8
- 230000000740 bleeding effect Effects 0.000 description 8
- 230000017074 necrotic cell death Effects 0.000 description 8
- 239000008187 granular material Substances 0.000 description 7
- 230000003118 histopathologic effect Effects 0.000 description 7
- 230000003902 lesion Effects 0.000 description 7
- 210000004165 myocardium Anatomy 0.000 description 7
- 230000007170 pathology Effects 0.000 description 7
- 206010059866 Drug resistance Diseases 0.000 description 6
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 238000011081 inoculation Methods 0.000 description 6
- 208000024891 symptom Diseases 0.000 description 6
- 230000001575 pathological effect Effects 0.000 description 5
- 244000144977 poultry Species 0.000 description 5
- 235000013594 poultry meat Nutrition 0.000 description 5
- 241000271566 Aves Species 0.000 description 4
- 206010020565 Hyperaemia Diseases 0.000 description 4
- 210000000683 abdominal cavity Anatomy 0.000 description 4
- 210000000436 anus Anatomy 0.000 description 4
- 210000000621 bronchi Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 230000004054 inflammatory process Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 210000000952 spleen Anatomy 0.000 description 4
- 239000000273 veterinary drug Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 229960003405 ciprofloxacin Drugs 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 235000021050 feed intake Nutrition 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000002440 hepatic effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 210000004400 mucous membrane Anatomy 0.000 description 3
- 210000002826 placenta Anatomy 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 238000002255 vaccination Methods 0.000 description 3
- 206010003445 Ascites Diseases 0.000 description 2
- 206010008631 Cholera Diseases 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 108010048581 Lysine decarboxylase Proteins 0.000 description 2
- 208000037273 Pathologic Processes Diseases 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 235000021053 average weight gain Nutrition 0.000 description 2
- 208000034158 bleeding Diseases 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000000994 depressogenic effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 210000002969 egg yolk Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 231100000518 lethal Toxicity 0.000 description 2
- 230000001665 lethal effect Effects 0.000 description 2
- 210000005229 liver cell Anatomy 0.000 description 2
- 210000003141 lower extremity Anatomy 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 210000004379 membrane Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000000116 mitigating effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000003097 mucus Anatomy 0.000 description 2
- 229940100688 oral solution Drugs 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000036285 pathological change Effects 0.000 description 2
- 231100000915 pathological change Toxicity 0.000 description 2
- 230000009054 pathological process Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000003716 rejuvenation Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 235000014347 soups Nutrition 0.000 description 2
- 230000003393 splenic effect Effects 0.000 description 2
- 239000000375 suspending agent Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 231100000816 toxic dose Toxicity 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 241000272814 Anser sp. Species 0.000 description 1
- 208000031504 Asymptomatic Infections Diseases 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 229930186147 Cephalosporin Natural products 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 244000118681 Iresine herbstii Species 0.000 description 1
- 206010024264 Lethargy Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 206010025476 Malabsorption Diseases 0.000 description 1
- 208000004155 Malabsorption Syndromes Diseases 0.000 description 1
- 206010061297 Mucosal erosion Diseases 0.000 description 1
- 206010028140 Mucous stools Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000773293 Rappaport Species 0.000 description 1
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 206010047924 Wheezing Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 208000038016 acute inflammation Diseases 0.000 description 1
- 230000006022 acute inflammation Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 229940126574 aminoglycoside antibiotic Drugs 0.000 description 1
- 239000002647 aminoglycoside antibiotic agent Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000004596 appetite loss Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 235000014590 basal diet Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 210000004413 cardiac myocyte Anatomy 0.000 description 1
- 229940124587 cephalosporin Drugs 0.000 description 1
- 150000001780 cephalosporins Chemical class 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000007012 clinical effect Effects 0.000 description 1
- 208000035850 clinical syndrome Diseases 0.000 description 1
- 210000003555 cloaca Anatomy 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000000630 fibrocyte Anatomy 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229940124307 fluoroquinolone Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000006194 liquid suspension Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000021266 loss of appetite Nutrition 0.000 description 1
- 208000019017 loss of appetite Diseases 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 1
- 229940107698 malachite green Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 231100000668 minimum lethal dose Toxicity 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 201000008383 nephritis Diseases 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000009374 poultry farming Methods 0.000 description 1
- 230000002633 protecting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000007665 sagging Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 208000037921 secondary disease Diseases 0.000 description 1
- 238000009589 serological test Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000007863 steatosis Effects 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 229940072172 tetracycline antibiotic Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000007817 turbidimetric assay Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention provides the purposes of pharmaceutical composition as described below in the drug of preparation prevention or treatment birds salmonellosis;Described pharmaceutical composition is the preparation being prepared by the bulk pharmaceutical chemicals of following weight proportion: 150-200 parts of radix scutellariae, 150-200 parts of Fructus Forsythiae, 150-200 parts of radix paeoniae rubra, 50-100 parts of the coptis, 250-350 parts of Cortex Phellodendri, 50-100 parts of Radix Glycyrrhizae.The research of the invention finds that, after the 6 taste medicine compatibility such as radix scutellariae, it can effectively prevent or treat the effect of birds salmonellosis, especially for Salmonella pullorum disease significant effect, it is suitable with positive drug effects of antibiotics, use of the alternative antibiotic in such disease provides possibility to avoid generating drug-fast bacteria.
Description
Technical field
The present invention relates to a kind of new applications of pharmaceutical composition.
Background technique
Salmonella is an important Pseudomonas in enterobacteriaceae, is widely present in nature, is in direct rod shape, no bud
Spore, Gram-negative.Salmonella in 1885 etc. is separated to Salmonella choleraesuls in Cholera Epidemic, therefore names as salmonella
Belong to.Salmonella has 2500 kinds or more of serotype, other than the serotype rare less than 10 belongs to Bang Geer salmonella,
Remaining serotype belongs to Salmonella enteritidis.Mainly there are Bacterium enteritidis, salmonella typhimurium, hog cholera Salmonella
Bacterium, S. pullonum, avian infectious bronchitis nephritis virus etc..It can cause a plurality of types of diseases, and and people to humans and animals
Class health, animal husbandry and the development relationship of international trade are close.
Salmonella pullon (Salmonella pullorum) is that Rettger divided from dysentery characterized by white mucous stool diseased chicken in 1899
From arriving.The white diarrhea as caused by it (Pullorum disease) is one of the poultry diease being found earliest.And the disease is confirmed to be
It is egg matchmaker's property disease.White diarrhea disease disease incidence is high, and chick is in break out more, and case fatality rate is high;Adult Chicken is in subclinical infection or chronic more
Infection, though without obvious clinical symptoms, caused by economic loss it is still very big.Therefore, anti-this disease of system is especially supported to intensive
The control of the chicken house disease is always the hot issue studied at present.Wherein drug controls the disease and is most effective and most common arranges
One of apply.However, the long-time service of single variety can induce Salmonella bar due to antibiotic and the irrational use of antimicrobial
Bacterium generates drug resistance, and the effect of common drug is more and more undesirable.Therefore effective preparation is researched and developed to control the disease and seem special
It is unimportant.
Summary of the invention
The purpose of the present invention is to provide a kind of new applications of pharmaceutical composition.
The present invention provides pharmaceutical compositions as described below in preparation prevention or the drug for the treatment of birds salmonellosis
In purposes;Described pharmaceutical composition is the preparation being prepared by the bulk pharmaceutical chemicals of following weight proportion:
150-200 parts of radix scutellariae, 150-200 parts of Fructus Forsythiae, 150-200 parts of radix paeoniae rubra, 50-100 parts of the coptis, 250-350 parts of Cortex Phellodendri,
50-100 parts of Radix Glycyrrhizae.
Further, the birds are chicken, duck or goose.
Further, the drug is the drug of prevention or treatment Salmonella pullorum disease.
Further, the drug is the drug of prevention or treatment Salmonella pullorum disease.
Further, it is the veterinary drug preparation being prepared by the bulk pharmaceutical chemicals of following weight proportion:
185 parts of radix scutellariae, 185 parts of Fructus Forsythiae, 185 parts of radix paeoniae rubra, 74 parts of the coptis, 297 parts of Cortex Phellodendri, 74 parts of Radix Glycyrrhizae.
Wherein, the drug is with the extraction of the water or organic solvent of the medicinal powder of the weight proportion bulk pharmaceutical chemicals or bulk pharmaceutical chemicals
Object is active constituent, in addition the preparation that pharmaceutically common auxiliary material or complementary ingredient are prepared.
By verification experimental verification, the powder that the medicinal powder of the present composition is directly prepared can effectively prevent or treat chicken
Salmonella pullorum disease can extract medicinal material to adapt to different requirements or reduce product dose, is pure
Change, has veterinary drug preparation of different nature in order to reduce dose or be made.
The veterinary drug preparation may be, but not limited to, injection, solution, decoction, preserved material, mixture, emulsion, aqua, leaching
Paste, eclegm, pulvis, pre-mixing agent, tablet, pill, capsule, microcapsules, granule, oral solution etc..
The research of the invention finds that have the function of preventing and treating birds salmonellosis after the 6 taste medicine compatibility such as radix scutellariae,
Suitable with positive drug effects of antibiotics especially for Salmonella pullorum disease significant effect, alternative antibiotic is at this
Use in class disease provides possibility to avoid generating drug-fast bacteria.
Detailed description of the invention
Result (HE was observed by the histopathologic slide after dissect in Fig. 1 healthy control group chick the 6th day;200 ×/400 ×):
A: cardiac muscle fibre arrangement is close, and core occupies center, the internal membrane of heart and external membrane of heart structural integrity.
B: liver lobuli hepatis structure is clear, and for central vein without extravasated blood, liver plate is radial, and sinus hepaticus is without expansion and oedema.
C: splenic capsule is complete, spleen trabeculae as it can be seen that acini lienalis negligible amounts, central artery sheath without hyperemia, in red pulp snius lienis without
Expansion, desmacyte is without hyperplasia.
D: for bronchuses at different levels as it can be seen that peribronchial only has individually a little cell infiltration, alveolar size is similar, interstitial without
Bleeding extravasated blood and fibrosis.
E: caecum wall construction is complete, it is seen that villus and crypts have no cell infiltration under mucosal erosion, mucous membrane.
F: caecum fluff structures are complete, it is seen that structural integrity crypts has no that obvious mucous epithelium falls off, and length is normal.
Fig. 2 infects the histopathologic slide observation result (HE after control group death chick dissect;200 ×/400 ×):
A: cardiac muscle fibre is disorganized, and core, which occupies, to disappear, Myocardial fibroklasts cavity.Show myocardium cell necrosis.And the visible heart
Extravasated blood.
B: liver lobuli hepatis structure is unintelligible, portal area cell infiltration, the radial disappearance of liver plate.
C: splenic capsule falls off, envelope necrosis.Cell infiltration.
D: brochial mucosa bleeding, cell infiltration.
E: caecum wall falls off with mucous membrane, and villus shortens, and crypts becomes smaller, and placenta percreta falls off necrosis.
F: caecum villus shortens, and crypts is reduced, it is seen that obvious mucous epithelium falls off.
Fig. 3 middle dosage intestines bacterium dissipates clearly group and dies of illness the observation result (HE of the histopathologic slide after chick dissect;200×/400
×):
A: cardiac muscle fibre arrangement is more neat, and part cavity occurs in endomyocardial side fibrocyte.Show myocardium cell necrosis.But
It is light compared with infection control group.
B: liver envelope structural integrity.Hepatic region cell is clear.
C: acini lienalis structure is substantially complete, has no obvious lesion tissue.
D: it is relatively cleaned in lung tissue bronchus, it is seen that lung tissue interstitial hemorrhage, cell infiltration.But it is bright compared with infection control group
It is aobvious slight.
E: caecum wall and envelope structural integrity, but see that caecum villus shortens compared with healthy control group, crypts becomes smaller, placenta percreta group
It knits and sees a small amount of slough tissue.It is obviously slight compared with infection control group.
Fig. 4 high dose intestines bacterium dissipates clearly group and dies of illness the observation result (HE of the histopathologic slide after chick dissect;200×/400
×):
A: cardiac muscle fibre arrangement is more neat, there is cell infiltration between cardiac muscle cell.But relatively infection control group is light.
B: liver envelope structural integrity.Hepatic region cell is clear.But central vein extravasated blood.
C: diagram liver plate is radial substantially complete, but sees liver cell nonresident portion steatosis.
D: acini lienalis structure is substantially complete, has no obvious lesion tissue.
E: cleaning in lung tissue bronchus, but visible bronchial tissue part consolidation.There is inflammation mistake suspected of preinfection
Repairing phase after journey.
F: caecum wall and envelope structural integrity, but see that caecum villus shortens compared with healthy control group, crypts becomes smaller, placenta percreta group
It knits and sees a small amount of slough tissue.It is obviously slight compared with infection control group.
Fig. 5 positive drug control group prevention group is died of illness the observation result (HE of the histopathologic slide after chick dissect;200×/
400 ×):
A: myocardium Non Apparent Abnormality.
B: liver cell structural integrity, portal area cell infiltration.
C: diagram liver plate is radial substantially complete, but sees that sinus hepaticus is expanded.
D: acini lienalis structure is substantially complete, has no obvious lesion tissue.
E: cleaning in lung tissue bronchus, but visible terminal bronchial tissue inner part bleeding and cell infiltration.Suspected of
There is the repairing phase after inflammatory process in infection.
F: caecum wall and envelope structural integrity, but see that caecum villus is elongated compared with healthy control group.Crypt depth deepens.
Fig. 6 diagram positive drug control group treatment group dies of illness the observation result (HE of the histopathologic slide after chick dissect;
200 ×/400 ×):
A: the obvious cell infiltration of cardiac muscular tissue.
B: part hepatic tissue hydropic degeneration.
C: bleeding around diagram lung tissue blood vessel.
D: lung tissue structure is substantially complete, and cleaning, without obvious bleeding and rheuminess object, has no obvious group in bronchial lumen
Knit lesion.
E: caecum envelope structural integrity, but see that caecum villus is elongated compared with healthy control group.Crypt depth deepens.
Specific embodiment
The preparation of the pharmaceutical composition of the present invention of embodiment 1
Radix scutellariae 185g, Fructus Forsythiae 185g, radix paeoniae rubra 185g, coptis 74g, Cortex Phellodendri 297g, Radix Glycyrrhizae 74g are taken, after crushing, mixes, does
It is dry to get powder.
The preparation of the pharmaceutical composition of the present invention of embodiment 2
Radix scutellariae 200g, Fructus Forsythiae 150g, radix paeoniae rubra 160g, coptis 50g, Cortex Phellodendri 250g, Radix Glycyrrhizae 50g are taken, after crushing, is mixed, then
Appropriate suspending agent is added, prepares suspension.
The preparation of the pharmaceutical composition of the present invention of embodiment 3
Radix scutellariae 150g, Fructus Forsythiae 200g, radix paeoniae rubra 200g, coptis 100g, Cortex Phellodendri 350g, Radix Glycyrrhizae 100g are taken, is added water to cook 3 times,
After concentration, appropriate filler is added, prepares granule.
The preparation of the pharmaceutical composition of the present invention of embodiment 4
Radix scutellariae 160g, Fructus Forsythiae 160g, radix paeoniae rubra 160g, coptis 90g, Cortex Phellodendri 330g, Radix Glycyrrhizae 90g are taken, 95% ethyl alcohol is added to extract 2
It is secondary, merge alcohol extract, the dregs of a decoction add water to cook 2 times, merge decocting liquid;After alcohol extract is recycled ethyl alcohol, merge with decocting liquid, it is spraying
It is dry, appropriate suspending agent is added, prepares suspension.
The preparation of the pharmaceutical composition of the present invention of embodiment 5
Radix scutellariae 190g, Fructus Forsythiae 190g, radix paeoniae rubra 190g, coptis 60g, Cortex Phellodendri 280g, Radix Glycyrrhizae 60g are taken, 60-65% ethyl alcohol is added to mention
It takes 3 times, merges alcohol extract, after recycling ethyl alcohol, spray drying is added appropriate amount of auxiliary materials, prepares granule.
The preparation of the pharmaceutical composition of the present invention of embodiment 6
Radix scutellariae 185g, Fructus Forsythiae 185g, radix paeoniae rubra 185g, coptis 74g, Cortex Phellodendri 297g, Radix Glycyrrhizae 74g are taken, is added water to cook 3 times, is closed
And decocting liquid, it is concentrated, stands, appropriate corrigent and bacteriostatic agent is added in filtration, filtrate, is prepared into oral solution.
Beneficial effects of the present invention are illustrated below by way of test example.
The therapeutic effect of 1 salmonellosis of test example
1 material and method
1.1 drugs and reagent
Pharmaceutical composition (also known as intestines bacterium dissipates clearly) of the present invention: by radix scutellariae, Fructus Forsythiae, the coptis, Cortex Phellodendri, radix paeoniae rubra and Radix Glycyrrhizae prescription
At
Radix scutellariae 213g Fructus Forsythiae 213g coptis 85g
Cortex Phellodendri 340g radix paeoniae rubra 213g Radix Glycyrrhizae 85g
12.2 preparation methods
Above 6 taste crushes, and is sieved, and mixes, it is dry to get.
According to above-mentioned medicinal material formula and preparation method, animal pharmaceutical estate Co., Ltd, Tongwei Co., Ltd. produces and provides
Three different batches inspection qualified products: lot number 20111031;20111032;20111033.The product is yellow powder.
It is provisional to be used by design dosage with dispensing is raised.
Ciprofloxacin;The sensible animal health Science and Technology Ltd. in Sichuan, specification: 50g:1g, lot number: 20120101.With feeding
Dispensing.
Salmonella pullon (Salmonella pullorum), strain CVCC519, C79-3, S.pu.2;Serum
Type are as follows: 9,121,123 :-:-, it is purchased from China Veterinery Drug Inspection Office;
Buffered peptone water (BP): it is voluntarily prepared by 4.12 regulations in GB4789.28;
Rappaport vassiliadis mdeium (MM) culture medium, Beijing overpass Technology Co., Ltd. lot number 120213;
Mai Kangkai culture medium, Beijing overpass Technology Co., Ltd. lot number 120213;
SS culture medium, Beijing overpass Technology Co., Ltd. lot number 120213;
XLD culture medium, Beijing overpass Technology Co., Ltd. lot number 120213;
Lysine decarboxylase test broth, Beijing overpass Technology Co., Ltd. lot number 120213;
Triple sugariron culture medium, Beijing overpass Technology Co., Ltd. lot number 120213;
A-F polyvalent serum, Lanzhou Institute of Biological Products
1.2 test apparatus
Electronic balance, Chengdu Puruixun Electronics Co., Ltd;
755B ultraviolet-uisible spectrophotometer, Shanghai essence tech equipment Co., Ltd;
Superclean bench, SuZhou Antai Air Tech Co., Ltd.;
VITEK-2 full automatic microorganism analysis system, France bioMerieux;
Genex liquid-transfering gun, Shanghai Wo Yuan Science and Technology Ltd..
Other routine experiment instruments.
1.3 experimental animal
Luo Man layer of 1 age in days after MD vaccine immunity 500, it is limited purchased from Chongqing City great Zu Qu Mingji poultry farming
Chicken house is planted by company.Freely drink the tap water let cool through scalding, and with the full price chicken feed for being free of any antibacterials
Raising selects the chick of 450 clinical examination health as subjects to 3 ages in days.By there is duplicate random district's groups
Animal is divided into 10 groups and tested by design, and weight and grouping situation are shown in Table 1.
1.4 feeds are free of the complete feed of any antibacterials.Purchased from the honest Co., Ltd in Zhengda Group Chongqing.
The grouping of 1 test chicken of table and disposition *
Number | Group | Number of animals | Weight | Processing |
1 | Healthy control group | 45 | 34.9±8.3 | Do not infect not medication, isolated rearing. |
2 | Infect control group | 45 | 37.1±6.7 | Infect not medication, isolated rearing. |
3 | C low dosage prevention group | 45 | 38.7±7.0 | 10mg·kg-1Weight, with raising, twice daily. |
4 | C middle dosage prevention group | 45 | 37.5±6.2 | 20mg·kg-1Weight, with raising, twice daily. |
5 | C high dose prevention group | 45 | 37.3±6.25 | 40mg·kg-1Weight, with raising, twice daily. |
6 | C low dose therapy group | 45 | 37.2±8.6 | 10mg·kg-1Weight, with raising, twice daily. |
7 | C middle dosage treatment group | 45 | 37.8±7.2 | 20mg·kg-1Weight, with raising, twice daily. |
8 | C high-dose therapy group | 45 | 37.3±6.45 | 40mg·kg-1Weight, with raising, twice daily. |
9 | CX positive drug prevention group | 45 | 37.4±6.6 | 20mg·kg-1Weight, with feeding, prevention administration. |
10 | CX positive drug treatment group | 45 | 37.8±6.2 | 40mg·kg-1Weight, with feeding, prevention administration. |
* 1, prevention group is 12h dispensing in advance, treats and is administered simultaneously for infection;
2, C represents intestines bacterium group scattered clearly;CX represents Ciprofloxacin group.
1.5 contamination bacterium solution preparations
Salmonella pullon (C79-13) be China Veterinary Drugs Supervisory Inst. reference culture.Egg is first inoculated in using preceding
36 ± 1 DEG C of culture 18h of white peptone buffer, then for 24 hours with 37 DEG C of scribing line cultures of ordinary flat, picking colonies typical is inoculated in common meat
37 DEG C of culture 18h, turbidimetric assay bacterial growth turbidity determine bacteria containing amount in soup.It is 6.75 × 10 by bacteria containing amount9The meat soup of a/L
Culture 0.25ml oral vaccination is non-to exempt from chicken rejuvenation, makes after the purifying agaric culture of the sterile separation rejuvenation of infection morbidity chicken liver
At contamination bacterium solution, and measuring quantitative bacterial concentration with Maxwell colorimetric method is 6.75 × 109A/L sets 4 DEG C of refrigerators and saves backup.
1.6 trial tests and the determination for attacking toxic dose
Before formal test, the 3 age in days healthy chicks for raising with field and raising under the same conditions are chosen, are connect by document is oral
Kind pathogenic strain, determines the minimal lethal dose MLD(10 of pathogenic strain9CFU/ is only).
Salmonella virulence prerun, takes a small amount of bacterium solution, makees 10 times of bacterium solutions for being diluted to various concentration with physiological saline, respectively
It is the 10 of stoste and stoste-1、10-2、10-3Concentration takes the close chicken of weight 50, is divided into 5 groups, every group 10, take orally respectively with
On not same amount or the bacterium solution of various concentration, observe chicken morbidity and death condition in 5d and record;The minimum lethal agent of 5 days animals
(MLD) is measured as this test and attacks toxic dose.
1.7 inoculum concentrations and bacterial population
Every chicken oral vaccination 1 × MLD salmonella bacterium solution.
1.7 dosages, the course for the treatment of and method
Each prevention group 12h before inoculation carries out preventive administration;When there is first morbidity chicken after inoculation in treatment group
Start to offer medicine;Infection control group fed does not add the basal diet of any drug;Healthy control group isolated rearing, does not appoint
Where reason.Freely drink cooling sterilizing ordinary water after boiling;The equal successive administration of each group 5 days, observation period are 8 days after inoculation.It gives
Pharmaceutical quantities, method are shown in Table 1.
1.7 index of assessment of curative effect
1.7.1 clinical observation
After animal inoculation pvaccination, in 1~8 day, clinical symptoms are observed day by day, and the state of mind, breath state, excrement, hind leg are closed
Section, amount of drinking water, feed intake etc. are observed, and carry out pathology dissect, bacterium separation identification to the chicken that dies of illness, and acquire chicken reality of dying of illness
Matter organ carries out the items contents such as pathological tissue observation and is tested, and observes and records test result.
1.7.2 the death rate
All to be prevented within experimental period or treatment is invalid, there is salmonellosis classical symptom and death in clinic, through cuing open
Cubing typical case's salmonellosis lesion characteristics, bacteriology separation, are accredited as positive, and it is dead to be determined as infection.According to death toll
Calculate each group death rate.
1.7.3 efficient
After the test, the percentage of test group is accounted in each group surviving animals as effective percentage.
1.7.4 cure rate
After medication, animal is without exception or restores normal, and no clinical symptoms person is to cure.It is controlled according to number calculating each group is cured
More rate.
1.7.5 feed intake
Each group feed intake is calculated with the difference of filling amount and surplus doses, and each test group is counted, it is average to obtain each group chicken
Feeding grain.Calculation formula is as follows: each group average daily gain (g)=(filling amount-remains doses)/number of animals
1.7.6 body weight increase rate
With the calculating of the ratio between the average weight gain of infection experiment group and the average weight gain of healthy control group, calculation formula is as follows:
1.7.7 the separation and identification of bacterium
Separation identification is carried out to bacterium by following check problem:
Sampling → Zengjing Granule → identification culture → biochemical investigation → serological test → result report.
1.7.7.1 acquisition and the Zengjing Granule of sample
The tested dead chicken of dissect, sterile separation simultaneously acquire liver blood, painstaking effort or ascites.Select broth medium and chlorine
Change magnesium malachite green bouillon (MM) culture based on Zengjing Granule 18 at 37 DEG C~for 24 hours, bacterium solution set 4 DEG C of refrigerators save it is to be checked.
1.7.7.2 identify the biochemical test of culture with bacterium
A small amount of Zengjing Granule bacterium solution is dipped with oese, streak inoculation is on XLD agar plate, Mai Kangkai culture medium.In
37 DEG C of culture 18h~for 24 hours, observation colony characteristics (it is recommended that morphological feature of description bacterium colony).Then, divide from XLD agar plate
Other picking meets 2 of salmonella cultural characteristic or more suspicious bacterium colonies, is seeded on triple sugar-iron-agar medium, first on inclined-plane
Scribing line is punctured then at bottom;Then it in the case where unsterilised after transfer needle inoculation, continues directly to be inoculated in lysine decarboxylase
Inoculated and cultured on test medium, 37 DEG C of culture 18h~for 24 hours, observe the growth characteristics of bacterium colony.It records and reports result.
Doubtful positive strain culture solution is carried out to biochemical identification, record in VITEK-2 full automatic microorganism analysis system
As a result.
1.8 data analysis and process
Selecting statistical software is that SPSS15.1 is for statistical analysis to data.The death rate, efficient chi-square criterion, weight gain are used
F is examined.
2. result
2.1 clinical effectiveness
Gradually appear different degrees of typical white scour of chicken clinical symptoms after Chickens Infected after 4h~8h, after 8h, chicken
Occur dead.Clinical disease one's own judgment: spirit is depressed or tired, and individual chicken pains pipe, and drinks loss of appetite, and loose random is exhaled
It inhales speedup, wheeze, both wings are sagging, and eye closing lethargic sleep, chilly gathers, and are reluctant to walk about, and draw white or green slurry shape loose stool, anus attached
Close or periphery faecal contamination, or stick white excrement blocking chick anus, chick group are often accompanied by sad and shrill shriek, in cage tool
Moist filthy, packing paper humidity has obvious loose stool.
Dead chicken pathology dissect major pathologic features are: liver hyperemia enlargement, being dispersed in property necrosis point, congestive heart,
There is intoxicated junket sample tubercle in hydropericardium, empsyxis or hyperemia, enteric hemorrhage, seroperitoneum, later period individual chick hearts, and hind leg closes
Save enlargement.Yolk malabsorption includes cheesy object, and intestinal mucosa falls off, and has a large amount of mucus shape whites to stagnate object in cloaca,
There are a large amount of mucus in oral cavity.From separation of bacterial such as liver, abdominal cavity, hearts, it is possible to identify infected for Salmonella.
Healthy control group chicken is during test without relevant clinical syndrome.
2.2 preliminary result
Preliminary result see the table below:
Table 2 attacks 5 days animal dead situations after malicious Salmonella pullorum
Table 2 the result shows that: the MLD of malicious chick is attacked through gastrointestinal tract as 0.25ml × 6.75 × 10 with the bacterial strain9A/L.
The disease incidence of 2.3 each test groups, the death rate, effective percentage, cure rate, weight gain, feed-weight ratio statistical result table.
The curative effect situation * of each test group of table 3
Note: data shoulder marking-up mother is identical in table, indicates that there was no significant difference (p > 0.05);Conversely, significant difference (p <
0.05/0.01)
As shown in Table 3, intestines bacterium dissipates clearly the prevention for the white scour of chicken, middle dosage and high dose and the positive group of infection and low
Significant difference (p < 0.05/0.01) is deposited between dosage group;Its prevention morbidity effect is suitable compared with positive drug control group, morbidity
Rate is without significant difference (p > 0.05).The death rate also shows similar result.I.e. high middle dose group, the chick of positive drug group are dead
Rate is died, it is lower compared with low dose group and infection control group, have significant difference (p < 0.05/0.01);Prevention administration obtains healing
Rate table 3 is shown: prevent the chick of high dose group in dissipating clearly to intestines bacterium, cure rate relatively infection positive controls when off-test and
Low dose group is high, has significant difference (p < 0.05), but lower compared with positive drug control.
Table 3 is also shown: intestines bacterium dissipates clearly for treating the white scour of chicken, the death of high middle dose group, positive drug control group
Rate is lower, has significant difference (p < 0.05) compared with low dose group, infection positive controls.Cure rate on the 5th is also higher, with sense
Dye control group and low dose group are compared, and are had significant difference (p < 0.05).But it is lower than positive drug group (p < 0.05).
To sum up experimental result prompts, the prevention and treatment that can be used for the white scour of chicken scattered clearly of intestines bacterium, under high, middle dosage
Activity is suitable with positive Antibiotics drug, shows in the prevention and treatment for the white scour of chicken, intestines bacterium clears can replace entirely clearly
For antibiotic usage.
2.4 death separation of bacterial identification results
By dead diseased chicken dissect, sterile coring blood, liver blood or abdominal cavity ascites culture and identification culture, result are as follows:
4 dead animal bacterium of table is separately cultured and identification result
As the result is shown: it is significantly high that salmonella death chicken separates positive rate infected group, low dosage intestines bacterium medication group scattered clearly
In middle dosage, high dose medication group and positive drug control group.It is further noted that: either intestines bacterium dissipates medication group also clearly
It is to there is different animals to divide in the painstaking effort of dead chicken, liver, lung or abdominal cavity in the dead chicken of positive drug control group
Bacterium is separated out, through Preliminary Identification in addition to the salmonella for thering is infection to attack poison, there are also Some Animals ehec infection, lethal original
Because that may have certain relationship with the secondary infection of Escherichia coli except having outside the Pass with salmonella infection.
2.5 death histopathologic slides analyze result (see Fig. 1~6)
To all groups die of illness chick acquire the heart, liver, spleen, lung, caecum carry out pathology section examination.Control group chick exists
It cuts open within 6th day and above-mentioned tissue is taken to carry out sections observation after killing.
As the result is shown: morbidity chick shows the pathologic processes such as inflammation, bleeding, extravasated blood, denaturation, the necrosis of each tissue.Its
In it is the most serious to infect control group chick pathological change of dying of illness.And intestines bacterium dissipates clearly middle dosage, the high dose control group of control group
Die of illness chick, overall pathology section examination, and basic pathology change procedure and the infected group chick that dies of illness are similar.But it can see
Out, relatively slight in the degree of pathological change, and individual tissues have rehabilitation after apparent inflammatory process to show.Certainly, therein
The lesion tissue of positive drug control group prevention group is the slightest.Thus as the result is shown: although dissipating prevention clearly using intestines bacterium and controlling
Treat the death that the white scour of chicken is still likely to occur chick.But there is basic pathology process caused by pathogenic bacteria lighter.In fact from when death
Between and the death rate also obtained corresponding evidence.Intestines bacterium, which dissipates clearly, has certain suppression to pathologic process caused by mitigation salmonellosis
Production is used.And preferable preventive and therapeutic effect thus can be played to the prevention and treatment white scour of chicken.
3. discussion and conclusion
3.1 Salmonella pullorum diseases (pullorum disease), abbreviation white diarrhea is by Salmonella pullon
The infectious disease that caused various age chickens often send out.This disease not only brings serious harm to poultry husbandry, results in significant economic losses,
And S. pullonum can be colonized in poultry intestinal tract, polluted egg or when processing poultry ketoboidies, polluted chicken, after
And enter human foods chain, become the potential source of mankind's salmonella infection.So pass of the prevention and treatment of white diarrhea by people
Note.
In recent years, some chicken disease experts think that white diarrhea should be listed in the first place of chicken disease.Studies have found that 1~4 day in recent years
Age day old chick disease incidence increased significantly, and the chicken morbidity and mortality within 2 week old are all very high.It is brought sternly to poultry husbandry
The harm of weight.Although antibiotic, antibacterials, such as clinically common cephalosporins semisynthetic antibiotics, aminoglycoside
Antibiotic, tetracycline antibiotics, fluoroquinolones antimicrobial DP finish, disulfonamide etc. have certain effect to the disease is prevented and treated
Fruit can effectively reduce the morbidity and mortality of white diarrhea.But as treatment be not thorough and long-term Irrational Use of Drugs caused by
The increase of antibody-resistant bacterium increases the difficulty for preventing and treating the disease.In addition drug resistance salmonella and salmonella and Escherichia coli it
Between drug resistance transmitting and diffusion, the infection of drug resistance salmonella has become the difficult point for preventing and treating the disease at present.And drug resistance
The wide-scale distribution of salmonella has become potential public health harm problem.Therefore carry out the prevention and treatment of white scour of chicken salmonellosis
Drug research has great importance.Wherein, Chinese medicine, especially Chinese medicine compound prescription are because its adverse reaction is small, be not easy to remain, do not pollute
Environment is not likely to produce unique advantage possessed by drug resistance, is expected to obtain increasingly important role in terms of preventing and treating the disease.
3.2 this test press 0.25ml × 6.75 × 10 to 3 ages in days test chick9A/L(1 × MLD) take orally C79-13It is husky
Men Shi bacillus liquid suspension replicates artificial DISEASE IN FLOCKS pathological model and obtains success.Infection morbidity chick is after oral vaccination
Phase secondary disease after 4h~8h.It is fallen ill, and chick Major Clinical symptoms include spirit are depressed, are short of breath, have difficulty in breathing, piping, arranging white
Serosity excrement, anus are blocked by serosity excrement, are dehydrated, is thin, and are occurred in succession after 8h~12h in turn dead.After death
Dissect is visible: serious dehydration, enteron aisle hyperemia, bleeding, and gassy in enteron aisle, mucous membrane changes in acute inflammation, and liver extravasated blood goes out
Blood, the canescence spotty necrosis point on the visible liver surface of individual chick, empsyxis, yolk bag absorb not congruent disease.Pathological section
It observes the major lesions such as the visible heart, lung, liver, spleen, caecum organ and the disease substantially such as different degrees of bleeding, denaturation, necrosis occurs
Reason process.It acquires morbidity chicken anus swab (excrement) separation of bacterial and dies of illness chicken gizzard, the heart, abdominal cavity separation of bacterial through bacteriology checking
Prove that morbidity chick and dead chick are mainly caused by attacking toadstool in relation to (see Table 4 for details;Fig. 1~6).This result explanation uses this examination
Artificial challenge's pathological model of proved recipe method duplication can guarantee being normally carried out for this test completely.
3.3 test results of the present invention show: intestines bacterium dissipates clearly middle and high dose application in the white scour of chicken case of artificial challenge,
Premix addition, is used in conjunction 5d, can effectively reduce Chickens Infected disease incidence, and infection chick morbidity case fatality rate is effectively reduced.With infection
Control group and low dose group, which are compared, has significant difference.And the protective effect, with positive control medicine Ciprofloxacin pre-mixing agent
Protecting effect is quite (see Table 2 for details).In addition test result is also shown: even if the dead chick after medication, also shows using height
After middle dosage intestines bacterium dissipates clearly, the morbidity death time can be slowed down, the pathology for mitigating metainfective intestines, liver, lung, spleen and caecum etc. becomes
Change (being detailed in Fig. 1~6).This result shows that: intestines bacterium clearly dissipate with prevention and treatment salmonella infection chick cause DISEASE IN FLOCKS compared with
Remarkable result.Test result affirmative intestines bacterium dissipates clearly the clinical effect for preventing and treating the white scour of chicken.
Claims (4)
1. the purposes of pharmaceutical composition as described below in the drug of preparation prevention or treatment Salmonella pullorum disease;It is described
Pharmaceutical composition is the preparation being prepared by the bulk pharmaceutical chemicals of following weight proportion:
185 parts of radix scutellariae, 185 parts of Fructus Forsythiae, 185 parts of radix paeoniae rubra, 74 parts of the coptis, 297 parts of Cortex Phellodendri, 74 parts of Radix Glycyrrhizae.
2. purposes according to claim 1, it is characterised in that: the drug is prevention or treatment Salmonella pullorum
The drug of disease.
3. purposes according to claim 1 or 2, it is characterised in that: the drug is with the weight proportion bulk pharmaceutical chemicals
The water or/and extractive with organic solvent of medicinal powder or bulk pharmaceutical chemicals be active constituent, in addition pharmaceutically common auxiliary material or it is complementary at
Divide the preparation being prepared.
4. purposes according to claim 3, it is characterised in that: the organic solvent is ethyl alcohol or hydrous ethanol.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410142650.XA CN104971124B (en) | 2014-04-10 | 2014-04-10 | A kind of new application of pharmaceutical composition |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410142650.XA CN104971124B (en) | 2014-04-10 | 2014-04-10 | A kind of new application of pharmaceutical composition |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104971124A CN104971124A (en) | 2015-10-14 |
CN104971124B true CN104971124B (en) | 2019-05-24 |
Family
ID=54268445
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410142650.XA Active CN104971124B (en) | 2014-04-10 | 2014-04-10 | A kind of new application of pharmaceutical composition |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104971124B (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107929414A (en) * | 2016-10-12 | 2018-04-20 | 葛洪伟 | It is a kind of that the pure Chinese medicine paste for reducing chick salmonella infection rate is administered by abdomen navel |
CN107137537A (en) * | 2017-05-04 | 2017-09-08 | 河北工程大学 | A kind of medicine for preventing and treating rabbit salmonellosis |
CN110403993B (en) * | 2019-07-30 | 2022-01-25 | 扬州大学 | Prescription and application of compound pulsatilla chinensis powder |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102988550A (en) * | 2012-10-15 | 2013-03-27 | 李正梅 | Preparation method and application of qinlian tablet |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101130139B1 (en) * | 2009-09-29 | 2012-03-28 | (주)양성그린바이오 | Composition for preventing calves diarrhea |
-
2014
- 2014-04-10 CN CN201410142650.XA patent/CN104971124B/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102988550A (en) * | 2012-10-15 | 2013-03-27 | 李正梅 | Preparation method and application of qinlian tablet |
Non-Patent Citations (2)
Title |
---|
22种中草药对畜禽常见肠道病原菌的体外抑菌作用;张莉等;《甘肃农业大学学报》;20121031;第47卷(第5期);第7-11页,第17页 |
蛋禽生殖系感染性炎症的诊治;蒋平等;《河南农业》;20041231(第3期);第34页 |
Also Published As
Publication number | Publication date |
---|---|
CN104971124A (en) | 2015-10-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102151256B (en) | Application of protocatechuic acid in preparation of drugs for preventing and controlling livestock and poultry virus infectious diseases | |
CN103006792B (en) | A kind of herbal medicine for preventing and treating livestock and poultry intestinal canal diseases and preparation method thereof and feed | |
CN103948930B (en) | A kind of poultry compound medicine containing Radix Paeoniae Rubra and beta-lactam antimicrobial drug | |
CN104971124B (en) | A kind of new application of pharmaceutical composition | |
CN102008667B (en) | Preparation method for traditional Chinese medicine composition with antibacterium and antiphlogosis efficacies | |
CN107158087A (en) | It is a kind of to prevent Chinese medicine composition of hog cholera and preparation method thereof | |
CN109568301A (en) | A kind of gallic acid, Rhein compound prescription preparation method and applications | |
CN102872307A (en) | Traditional Chinese medicine oral liquid for preventing and treating virus diseases of poultry and method for preparing traditional Chinese medicine oral liquid | |
CN107661416A (en) | A kind of traditional Chinese medicine extraction compound pharmaceutical for preventing and treating livestock and birds respiratory disease | |
CN105709081A (en) | Pharmaceutical composition used for treating infectious rhinitis of chicken, applications thereof, and feed | |
CN104523897B (en) | A kind of enema medicament composition for treating piglet epidemic diarrhea | |
CN102038881B (en) | Traditional Chinese medicine composition with antibacterial and antiphlogistic effect | |
CN105343229A (en) | Traditional Chinese medicinal composition for preventing and treating colibacillosis of livestock and poultry, and preparation method thereof | |
CN101209329A (en) | Chinese medicinal oral liquid with heat-clearing and detoxication, lung-heat-clearing and cough-relieving efficacy | |
CN101972325A (en) | Medicament composition for treating chick coccidiosis and preparation method thereof | |
CN101129379A (en) | Anticoccidiosis pharmaceutical composition and method of preparing the same | |
CN102293764B (en) | New application of p-hydroxycinnamic acid | |
CN101530405A (en) | Citric acid and compound citric acid application in pharmacy | |
KR101842668B1 (en) | Novel Salmonella specific becteriophage SG2 and antibacterial composition comprising the same | |
CN109620877A (en) | Pure Chinese medicine fowl antibacterium disease preparation and preparation method thereof and application method | |
CN115252668B (en) | Application of senecio scandens water extract as unique active ingredient in preparation of oral medicine for preventing and treating colibacillosis of chicken-eating type | |
CN104606627B (en) | A kind of pharmaceutical composition for treating grice diarrhoea and preparation method thereof | |
CN110075131A (en) | Application of the zika virus attenuated strain in treatment glioma | |
CN102309643A (en) | Chinese medicine composition for treating chicken coccidiosis | |
CN101797345A (en) | Traditional Chinese medicine composition for preventing and treating viral diseases of poultry and preparation method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231106 Address after: No. 588, Middle Tianfu Avenue, Chengdu Hi tech Zone, China (Sichuan) Pilot Free Trade Zone, 610000, Sichuan Patentee after: Tongwei Agricultural Development Co.,Ltd. Address before: 610000 No. 11, section 4, south 2nd Ring Road, hi tech Zone, Chengdu, Sichuan Patentee before: TONGWEI Co.,Ltd. |
|
TR01 | Transfer of patent right |