CN104970201B - A kind of production method of the fermented bean dregs rich in arginine and lysine - Google Patents
A kind of production method of the fermented bean dregs rich in arginine and lysine Download PDFInfo
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- CN104970201B CN104970201B CN201510417312.7A CN201510417312A CN104970201B CN 104970201 B CN104970201 B CN 104970201B CN 201510417312 A CN201510417312 A CN 201510417312A CN 104970201 B CN104970201 B CN 104970201B
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- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 100
- 235000010627 Phaseolus vulgaris Nutrition 0.000 title claims abstract description 100
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 239000004472 Lysine Substances 0.000 title claims abstract description 41
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 30
- 239000004475 Arginine Substances 0.000 title claims abstract description 22
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 6
- 238000000855 fermentation Methods 0.000 claims description 51
- 230000004151 fermentation Effects 0.000 claims description 34
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 32
- 230000001954 sterilising effect Effects 0.000 claims description 28
- 235000019764 Soybean Meal Nutrition 0.000 claims description 21
- 239000002002 slurry Substances 0.000 claims description 21
- 239000004455 soybean meal Substances 0.000 claims description 21
- CANRESZKMUPMAE-UHFFFAOYSA-L Zinc lactate Chemical compound [Zn+2].CC(O)C([O-])=O.CC(O)C([O-])=O CANRESZKMUPMAE-UHFFFAOYSA-L 0.000 claims description 20
- 235000000193 zinc lactate Nutrition 0.000 claims description 20
- 239000011576 zinc lactate Substances 0.000 claims description 20
- 229940050168 zinc lactate Drugs 0.000 claims description 20
- 239000002994 raw material Substances 0.000 claims description 18
- PMVSDNDAUGGCCE-TYYBGVCCSA-L Ferrous fumarate Chemical compound [Fe+2].[O-]C(=O)\C=C\C([O-])=O PMVSDNDAUGGCCE-TYYBGVCCSA-L 0.000 claims description 17
- 235000002332 ferrous fumarate Nutrition 0.000 claims description 17
- 239000011773 ferrous fumarate Substances 0.000 claims description 17
- 229960000225 ferrous fumarate Drugs 0.000 claims description 17
- 235000015393 sodium molybdate Nutrition 0.000 claims description 17
- 239000011684 sodium molybdate Substances 0.000 claims description 17
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 17
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims description 16
- 229960003390 magnesium sulfate Drugs 0.000 claims description 16
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 16
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 16
- 235000019157 thiamine Nutrition 0.000 claims description 16
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims description 16
- 229960003495 thiamine Drugs 0.000 claims description 16
- 239000011721 thiamine Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000002361 compost Substances 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 8
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 8
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 8
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 8
- 241000186015 Bifidobacterium longum subsp. infantis Species 0.000 claims description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 7
- 229940004120 bifidobacterium infantis Drugs 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 239000002131 composite material Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 28
- 150000001413 amino acids Chemical class 0.000 abstract description 17
- 241000251468 Actinopterygii Species 0.000 abstract description 10
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 238000009360 aquaculture Methods 0.000 abstract description 4
- 235000013406 prebiotics Nutrition 0.000 abstract description 3
- 230000003053 immunization Effects 0.000 abstract 1
- 235000009697 arginine Nutrition 0.000 description 17
- 235000001014 amino acid Nutrition 0.000 description 16
- 230000000052 comparative effect Effects 0.000 description 8
- 241000194108 Bacillus licheniformis Species 0.000 description 7
- 239000003797 essential amino acid Substances 0.000 description 6
- 235000020776 essential amino acid Nutrition 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 235000019733 Fish meal Nutrition 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000186012 Bifidobacterium breve Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- 150000008535 L-arginines Chemical class 0.000 description 1
- 241001275898 Mylopharyngodon piceus Species 0.000 description 1
- 238000004497 NIR spectroscopy Methods 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000013527 bean curd Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000005792 cardiovascular activity Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- VLAPMBHFAWRUQP-UHFFFAOYSA-L molybdic acid Chemical compound O[Mo](O)(=O)=O VLAPMBHFAWRUQP-UHFFFAOYSA-L 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229940083542 sodium Drugs 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
Arginine and lysine cannot meet Animal needs in nutritional need and existing dregs of beans based on animal, the particularly nutritional need of aquiculture animal, the present situation of growth of animal is limited, the present invention is intended to provide a kind of fermented bean dregs and its production method rich in arginine and lysine.The production method of the fermented bean dregs of the present invention can effectively improve the content of arginine and lysine in dregs of beans, improve the economic value of dregs of beans, for Feed Manufacturing, it can reduce production cost, simplify technique, for cultivated animals, particularly aquiculture animal, particularly for fish, it can meet the needs of it is to limiting amino acid, while play prebiotic left and right, improve the upgrowth situation of animal, improve animal immunizing power.
Description
Technical field
The invention belongs to field of fodder, also relates to microbial fermentation technology, and in particular to one kind rich in arginine and
The production method of the fermented bean dregs of lysine.
Background technology
For animal, amino acid can be divided into two classes, and one kind is essential amino acid, and another kind of is nonessential amino acid；
For feed processing, in 10 kinds of essential amino acids that feed is provided, if some amino acid especially lack, it can limit
The utilization rate of other essential amino acids is made, and determines the effective rate of utilization of whole feed protein, is referred to as limiting amino acid.
Size of the limiting amino acid according to its restriction effect in feed, is known as first, second or third limiting amino acid.For
Each feed protein, first, second or third limiting amino acid of various animals is probably different, is supported for aquatic products
Animal is grown, particularly for fish, first, second limiting amino acid is arginine or lysine respectively in fish meal, for
For pig, its first, second limiting amino acid is lysine or methionine.
In general, forage protein utilization rate can be improved by adding synthesizing amino acid in feed, but if not
The first limiting amino acids are added, the second or the 3rd limiting amino acid of addition is ineffective.In this regard, there is a kind of " bucket
It is theoretical " do vivid explanation：10 kinds of essential amino acids are compared to surround 10 pieces of planks of a wooden barrel bucket wall, this bucket can fill
How much water（Equivalent to effective trophic level of protein）Depending on that shortest block plank（Equivalent to the first restricted amino
Acid）, length is also of no avail again for other planks.Lysine is the most essential amino acid of animal body requirement, and animal body is completely not
Oneself it can synthesize, the requirement for particularly growing animal to lysine is high, and the speed of growth is faster, and growth pressure is higher, then needs
Lysine it is also more.Content is relatively low in most animals common feedstuffs raw material, therefore is the first or second limiting amino acid, its
Effect be improve a poor appetite, enhancing development；Promote the healing of wound, fracture etc., strengthen the resistance to various infectious diseases.Essence
Propylhomoserin is one of essential amino acid of fish, it participates in protein in fish body, and the synthesis of polyamines, promotes growth;Arginine has
Have and promote endocrine effect;Arginine can generate nitric oxide in vivo, and nitric oxide participates in fish cardiovascular activity, immune function
Adjusting.
The content of the invention
Lysine and arginine cannot meet Animal needs in nutritional need and existing dregs of beans based on animal, and limitation is dynamic
The present situation of thing growth, the present invention is intended to provide a kind of be rich in lysine and arginic fermented bean dregs and its production method.
To solve above technical problem, the present invention adopts the following technical scheme that：
A kind of production method rich in lysine and arginic fermented bean dregs, it is characterised in that comprise the following steps：
Step 1：Prepare dregs of beans raw material；
Step 2：Water is added in dregs of beans raw material and stirs evenly to obtain soybean meal slurry, wherein material water weight ratio is：1:0.5
~1.0；
Step 3：The soybean meal slurry that step 2 is obtained carries out sterilization treatment, and sterilising temp is 135~144 DEG C, during sterilizing
Between be 2-3min, naturally cool to room temperature after sterilizing；
Step 4：Soybean meal slurry after step 3 sterilizes is added into sodium molybdate, zinc lactate, magnesium sulfate, thiamine（Dimension
Raw element B1）And ferrous fumarate, the addition of wherein sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate are respectively
0.3-0.5%, 0.1-0.3%, 0.2-0.4%, 1-2% and 0.4-0.7% of dregs of beans weight, and stir evenly to obtain dregs of beans compost；
Step 5：Bacillus subtilis, bacillus licheniformis bacillus are accessed in the dregs of beans compost obtained through step 4
And saccharomyces cerevisiae, be passed through at 32-35 DEG C oxygen fermentation 36-48 it is small when, obtain bean pulp fermentation thing；
Step 6：Lactobacillus plantarum and bifidobacterium infantis are added in the bean pulp fermentation thing to ferment through step 5,
After when anaerobic fermentation 24-30 is small at 30-32 DEG C, 25-28 DEG C, when continuation anaerobic fermentation 36-48 is small is cooled to, obtains fermentation production
Thing；
Step 7：The tunning to ferment by step 6 is dried, up to arginine and lysine is rich in
Fermented bean dregs.
Preferably, the addition point of sodium molybdate in the step 4, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate
Not Wei dregs of beans weight 0.4%, 0.2%, 0.3%, 1.2% and 0.6%.
Preferably, be in the step 5 be passed through at 33 DEG C oxygen fermentation 40 it is small when.
Preferably, be in the step 6 when anaerobic fermentation 28 is small at 31 DEG C after, be cooled to 26 DEG C, continue anaerobic fermentation
40 it is small when.
Preferably, the dry selection low temperature drying in the step 7, such as dried at a temperature of 45 DEG C.
The invention has the advantages that and beneficial effect：
First, the method that the present invention uses biologically fermenting, passes through bacillus subtilis, bacillus licheniformis bacillus and wine
The composite fermentation of brewer yeast, lactobacillus plantarum and bifidobacterium breve so that the nutritional ingredient of dregs of beans is improved, and effectively increases
Lysine and arginic content in dregs of beans.
Secondly, sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate are with the addition of in fermentation substrate of the invention,
Itself and dregs of beans fermentation for composite, the arginic content being effectively improved in fermented bean dregs, five be used cooperatively are played
Unexpected effect, its with do not add or the scheme of part addition compared with, substantially reduce fermentation time, while also pole
The earth improves lysine and arginic yield, this has no report in the prior art, belongs to unexpected technique effect.
Again, the fermented bean dregs produced by the production method of the present invention, it has been rich in lysine and arginine, is using
No longer need additionally to strengthen lysine and arginine in the production of the mixed feed of the dregs of beans, you can meet animal, particularly water
Animal is produced to lysine and arginic demand, significantly reduces Feed Manufacturing cost, while cause the production technology of feed
It is simplified；In addition, the fermented bean dregs of the present invention belong to conventional probiotic microorganisms using microorganism, remaining viable bacteria or
Spore can activate in animal intestinal tract, play certain prebiotic left and right；Meanwhile substantial amounts of dimension is generated by microbial fermentation
Raw element, particularly B family vitamin, help to improve the upgrowth situation of animal and improve immunity.
To sum up, the production method of the fermented bean dregs of the present invention can effectively improve in dregs of beans arginine and arginic
Content, improves the economic value of dregs of beans, and for Feed Manufacturing, it can reduce production cost, simplify technique, for
For cultivated animals, particularly for aquiculture animal, particularly fish, it can meet animal to limiting amino acid
Demand, while prebiotic left and right is played, improve the upgrowth situation of animal, improve animal survival rate.
Embodiment
Embodiment 1：A kind of production method rich in lysine and arginic fermented bean dregs, it is characterised in that including with
Lower step：
Step 1：Prepare dregs of beans raw material；
Step 2：Water is added in dregs of beans raw material and stirs evenly to obtain soybean meal slurry, wherein material water weight ratio is：1:0.8；
Step 3：The soybean meal slurry that step 2 is obtained carries out sterilization treatment, and sterilising temp is 144 DEG C, and sterilization time is
2min, room temperature is naturally cooled to after sterilizing；
Step 4：Soybean meal slurry after step 3 sterilizes is added into sodium molybdate, zinc lactate, magnesium sulfate, thiamine（Dimension
Raw element B1）And ferrous fumarate, the addition of wherein sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate are respectively
0.4%, 0.2%, 0.3%, 1.2% and the 0.6% of dregs of beans weight, and stir evenly to obtain dregs of beans compost；
Step 5：Bacillus subtilis, bacillus licheniformis bacillus are accessed in the dregs of beans compost obtained through step 4
And saccharomyces cerevisiae, be passed through at 33 DEG C oxygen fermentation 40 it is small when, obtain bean pulp fermentation thing；
Step 6：Lactobacillus plantarum and bifidobacterium infantis are added in the bean pulp fermentation thing to ferment through step 5,
After when anaerobic fermentation 28 is small at 31 DEG C, 26 DEG C are cooled to, when continuation anaerobic fermentation 40 is small, obtains tunning；
Step 7：The tunning to ferment by step 6 is dried（Dried at a temperature of 45 DEG C
It is dry）, up to arginine and fermenting lysine dregs of beans is rich in.
Embodiment 2：A kind of production method rich in lysine and arginic fermented bean dregs, it is characterised in that including with
Lower step：
Step 1：Prepare dregs of beans raw material；
Step 2：Water is added in dregs of beans raw material and stirs evenly to obtain soybean meal slurry, wherein material water weight ratio is：1:0.8；
Step 3：The soybean meal slurry that step 2 is obtained carries out sterilization treatment, and sterilising temp is 135 DEG C, and sterilization time is
3min, room temperature is naturally cooled to after sterilizing；
Step 4：Soybean meal slurry after step 3 sterilizes is added into sodium molybdate, zinc lactate, magnesium sulfate, thiamine（Dimension
Raw element B1）And ferrous fumarate, the addition of wherein sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate are respectively
0.3%, 0.3%, 0.2%, 2% and the 0.4% of dregs of beans weight, and stir evenly to obtain dregs of beans compost；
Step 5：Bacillus subtilis, bacillus licheniformis bacillus are accessed in the dregs of beans compost obtained through step 4
And saccharomyces cerevisiae, be passed through at 32 DEG C oxygen fermentation 48 it is small when, obtain bean pulp fermentation thing；
Step 6：Lactobacillus plantarum and bifidobacterium infantis are added in the bean pulp fermentation thing to ferment through step 5,
After when anaerobic fermentation 30 is small at 30 DEG C, 25 DEG C are cooled to, when continuation anaerobic fermentation 48 is small, obtains tunning；
Step 7：The tunning to ferment by step 6 is dried, up to arginine and lysine is rich in
Fermented bean dregs.
Embodiment 3：A kind of production method rich in lysine and arginic fermented bean dregs, it is characterised in that including with
Lower step：
Step 1：Prepare dregs of beans raw material；
Step 2：Water is added in dregs of beans raw material and stirs evenly to obtain soybean meal slurry, wherein material water weight ratio is：1:0.7；
Step 3：The soybean meal slurry that step 2 is obtained carries out sterilization treatment, and sterilising temp is 137 DEG C, and sterilization time is
2min, room temperature is naturally cooled to after sterilizing；
Step 4：Soybean meal slurry after step 3 sterilizes is added into sodium molybdate, zinc lactate, magnesium sulfate, thiamine（Dimension
Raw element B1）And ferrous fumarate, the addition of wherein sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate are respectively
0.5%, 0.1%, 0.4%, 1% and the 0.4% of dregs of beans weight, and stir evenly to obtain dregs of beans compost；
Step 5：Bacillus subtilis, bacillus licheniformis bacillus are accessed in the dregs of beans compost obtained through step 4
And saccharomyces cerevisiae, be passed through at 35 DEG C oxygen fermentation 36 it is small when, obtain bean pulp fermentation thing；
Step 6：Lactobacillus plantarum and bifidobacterium infantis are added in the bean pulp fermentation thing to ferment through step 5,
After when anaerobic fermentation 24 is small at 32 DEG C, 28 DEG C are cooled to, when continuation anaerobic fermentation 36 is small, obtains tunning；
Step 7：The tunning to ferment by step 6 is dried, up to arginine and lysine is rich in
Fermented bean dregs.
Embodiment 4：Comparative example
Comparative example 1：A kind of production method of fermented bean dregs, comprises the following steps：
Step 1：Prepare dregs of beans raw material；
Step 2：Water is added in dregs of beans raw material and stirs evenly to obtain soybean meal slurry, wherein material water weight ratio is：1:0.8；
Step 3：The soybean meal slurry that step 2 is obtained carries out sterilization treatment, and sterilising temp is 144 DEG C, and sterilization time is
2min, room temperature is naturally cooled to after sterilizing；
Step 4：Soybean meal slurry after step 3 sterilizes is added into zinc lactate, the wherein addition of zinc lactate is beans
The 0.2% of dregs of rice weight, and stir evenly to obtain dregs of beans compost；
Step 5：Bacillus subtilis, bacillus licheniformis bacillus are accessed in the dregs of beans compost obtained through step 4
And saccharomyces cerevisiae, be passed through at 33 DEG C oxygen fermentation 40 it is small when, obtain bean pulp fermentation thing；
Step 6：Lactobacillus plantarum and bifidobacterium infantis are added in the bean pulp fermentation thing to ferment through step 5,
After when anaerobic fermentation 28 is small at 31 DEG C, 26 DEG C are cooled to, when continuation anaerobic fermentation 40 is small, obtains tunning；
Step 7：The tunning to ferment by step 6 is dried（Dried at a temperature of 45 DEG C
It is dry）, to obtain the final product.
Comparative example 2：A kind of production method of fermented bean dregs, comprises the following steps：
Step 1：Prepare dregs of beans raw material；
Step 2：Water is added in dregs of beans raw material and stirs evenly to obtain soybean meal slurry, wherein material water weight ratio is：1:0.8；
Step 3：The soybean meal slurry that step 2 is obtained carries out sterilization treatment, and sterilising temp is 144 DEG C, and sterilization time is
2min, room temperature is naturally cooled to after sterilizing；
Step 4：Soybean meal slurry after step 3 sterilizes is added into sodium molybdate, zinc lactate and ferrous fumarate, wherein
The addition of sodium molybdate, zinc lactate and ferrous fumarate is respectively 0.4%, 0.2% and the 0.6% of dregs of beans weight, and is stirred evenly
Obtain dregs of beans compost；
Step 5：Bacillus subtilis, bacillus licheniformis bacillus are accessed in the dregs of beans compost obtained through step 4
And saccharomyces cerevisiae, be passed through at 33 DEG C oxygen fermentation 40 it is small when, obtain bean pulp fermentation thing；
Step 6：Lactobacillus plantarum and bifidobacterium infantis are added in the bean pulp fermentation thing to ferment through step 5,
After when anaerobic fermentation 28 is small at 31 DEG C, 26 DEG C are cooled to, when continuation anaerobic fermentation 40 is small, obtains tunning；
Step 7：The tunning to ferment by step 6 is dried（Dried at a temperature of 45 DEG C
It is dry）, up to arginine and fermenting lysine dregs of beans is rich in.
Embodiment 5：
On the basis of the comparative examples of embodiment 1-3 and embodiment 4, foundation《GB/T 18868-2002 feeds
Middle moisture, thick protein, crude fibre, crude fat, lysine, methionine quickly measure near infrared spectroscopy》And《GB
28306-2012 national food safety standard food additives L-arginines》Method measure fermented bean dregs in arginine with
The content of lysine, concrete outcome such as following table：
| Handle sample | Lysine mass percentage | Arginine mass percentage |
| Raw material dregs of beans | 2.92 | 3.81 |
| Embodiment 1 | 11.27 | 14.74 |
| Embodiment 2 | 10.66 | 13.45 |
| Embodiment 3 | 10.13 | 12.97 |
| Comparative example 1 | 4.79 | 5.12 |
| Comparative example 2 | 5.21 | 6.37 |
From above measurement result, compared to raw material dregs of beans, lysine content improves in the fermentation skin of beancurd of embodiment 1-3
4-5 times, up to 11.27%, arginic mass percentage improves 3-4 times or so, and up to 14.74%, fermentation
Aquiculture animal is rich in dregs of beans, particularly the lysine and arginine needed for fish, effectively improves the nutrition of dregs of beans
Value.By the comparison of comparative example, guessing with small-scale fermentation test as a result, due to molybdic acid for inventor is also demonstrated
The compound addition of sodium, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is effectively improved lysine and arginic production
Amount, this point are never reported in the prior art；From comparative result, particularly sodium molybdate, zinc lactate, magnesium sulfate,
Thiamine and the addition of ferrous fumarate, it can be greatly enhanced lysine and arginic content.
Embodiment 6：
The product of the embodiment of the present invention 1 is tried out in fishery cultivating base, the object of feeding is the mixed breeding pond of carp and black carp,
Compare feeding through peasant household, it is found that the fish speed of growth of fermented bean dregs feeding using the present invention is faster than the fish of normal meal feeding,
And due to being not required to need to strengthen amino acid and enzyme, through adjusting, the production cost of feed per ton reduces 4-8% or so, greatly saves
About production cost；And by statistics, in net cage the survival rate of fish compared to traditional dregs of beans or commercially available fermented bean dregs or
The survival rate of person's fish meal is higher by 3-7 percentage points, drastically increases the survival rate of feeding animals.
Raw material according to the present invention is the convenient source and strain that the prior art can be commercially available.In addition should manage
Solution, after reading the above teachings of the present invention, those skilled in the art can make the present invention various changes or repair
Change, these equivalent forms also fall within the scope of the appended claims of the present application.
Claims (6)
1. a kind of production method rich in lysine and arginic fermented bean dregs, it is characterised in that comprise the following steps：Step
One：Prepare dregs of beans raw material；Step 2：Water is added in dregs of beans raw material and stirs evenly to obtain soybean meal slurry, wherein material water weight ratio
For：1:0.5~1.0；Step 3：The soybean meal slurry that step 2 is obtained carries out sterilization treatment, and sterilising temp is 135~144 DEG C,
Sterilization time is 2-3min, and room temperature is naturally cooled to after sterilizing；Step 4：Soybean meal slurry after step 3 sterilizes is added
Sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate, wherein sodium molybdate, zinc lactate, magnesium sulfate, thiamine and Fu Ma
Sour ferrous addition is respectively 0.3-0.5%, 0.1-0.3%, 0.2-0.4%, 1-2% and 0.4-0.7% of dregs of beans weight, and stirs
Mix uniform dregs of beans compost；Step 5：Bacillus subtilis, lichens bud are accessed in the dregs of beans compost obtained through step 4
Spore bacillus bacillus and saccharomyces cerevisiae, be passed through at 32-35 DEG C oxygen fermentation 36-48 it is small when, obtain bean pulp fermentation thing；Step 6：
Lactobacillus plantarum and bifidobacterium infantis, the anaerobism at 30-32 DEG C are added in the bean pulp fermentation thing to ferment through step 5
After when the 24-30 that ferments is small, 25-28 DEG C is cooled to, when continuation anaerobic fermentation 36-48 is small, obtains tunning；Step 7：Will be through
Cross the tunning that step 6 is fermented and carry out low temperature drying, up to arginine and fermenting lysine dregs of beans is rich in；
Sodium molybdate, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate are with the addition of in the dregs of beans compost of the step 4, with
Dregs of beans fermentation for composite, the arginic content being effectively improved in fermented bean dregs.
2. the production method according to claim 1 rich in lysine and arginic fermented bean dregs, it is characterised in that institute
The addition for stating sodium molybdate in step 4, zinc lactate, magnesium sulfate, thiamine and ferrous fumarate is respectively dregs of beans weight
0.4%th, 0.2%, 0.3%, 1.2% and 0.6%.
3. the production method according to claim 1 rich in lysine and arginic fermented bean dregs, it is characterised in that institute
State in step 5 be passed through at 33 DEG C oxygen fermentation 40 it is small when.
4. the production method according to claim 1 rich in lysine and arginic fermented bean dregs, it is characterised in that institute
State in step 6 be when anaerobic fermentation 28 is small at 31 DEG C after, be cooled to 26 DEG C, continue anaerobic fermentation 40 it is small when.
5. the production method according to claim 1 rich in lysine and arginic fermented bean dregs, it is characterised in that institute
Stating the low temperature drying in step 7 is dried at a temperature of 45 DEG C.
6. one kind is rich in lysine and arginic fermented bean dregs, it is characterised in that it is through claim 1-5 any one institute
The method stated is prepared.
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| CN101715876B (en) * | 2009-04-13 | 2011-12-21 | 四川渴望生物科技有限公司 | Preparation method of high-efficiency compound microorganism soybean meal leaven |
| CN102763769A (en) * | 2012-08-06 | 2012-11-07 | 广东希普生物科技股份有限公司 | Method for preparing lysine-rich fermented soybean meal |
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| CN101715876B (en) * | 2009-04-13 | 2011-12-21 | 四川渴望生物科技有限公司 | Preparation method of high-efficiency compound microorganism soybean meal leaven |
| CN102763769A (en) * | 2012-08-06 | 2012-11-07 | 广东希普生物科技股份有限公司 | Method for preparing lysine-rich fermented soybean meal |
| CN103947906A (en) * | 2014-05-21 | 2014-07-30 | 双胞胎（集团）股份有限公司 | Pig microecological preparation and preparation method thereof |
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